Rh4CG123600

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0018830
Physical Location & Seq
Forward (+)
23897320 .. 23897832
513 bp
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UTR
Exon/CDS
Intron
Rh4CG123600.1

Sequence Viewer

Length: 273 bp
ATGGCTTCTTTCAAATCTGCTCTTTCACTGATGCTGCTTGCTGTACCTCTTGTTGTTATGGTTGCCTCTGCGGGTAACTTCAACCAGGACTTTCAAATTACATGGGGAGATGGCCGAGCCAAGATACTCAACAATGGCCAACTTCTTACTCTCACGCTCGACAAAGCCTCTGGCTCTGGTTTCGAATCCAAAAATGAATATCTTTTCGGCAAAATCGATATGCAGCTCAAGCTTGTTGCTGGGAACTCTGCTGGAACTGTCACCGCCTACTAT

Protein Analysis

91

Amino Acids

9.72

Weight (kDa)

8.01

Isoelectric Point (pI)

1.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 27 - 91 1.4e-14 Glycosyl hydrolases family 16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018830)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g09151
pyrus_communis pycom09g07180
rosa_chinensis RchiOBHm_Chr4g0402941
rosa_laevigata RLG00000009038
rosa_samantha Rh2BG503900 Rh4AG116400 Rh4CG123600 Rh4CG123800 Rh4CG185600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 71, 264
AcoI YGGCCR 2 cut(s) 112, 136
AfaI GTAC 1 cut(s) 45
AgsI TTSAA 3 cut(s) 13, 82, 95
AjnI CCWGG 1 cut(s) 84
AluBI AGCT 2 cut(s) 226, 232
AluI AGCT 2 cut(s) 226, 232
AoxI GGCC 2 cut(s) 112, 136
ApeKI GCWGC 2 cut(s) 34, 223
AsuHPI GGTGA 1 cut(s) 253
AsuII TTCGAA 1 cut(s) 183
BalI TGGCCA 1 cut(s) 138
BbvI GCAGC 2 cut(s) 21, 235
BccI CCATC 1 cut(s) 104
BciT130I CCWGG 1 cut(s) 86
BisI GCNGC 2 cut(s) 35, 224
BlsI GCNGC 2 cut(s) 36, 225
Bme1390I CCNGG 1 cut(s) 86
BmrFI CCNGG 1 cut(s) 86
BmsI GCATC 1 cut(s) 21
Bpu14I TTCGAA 1 cut(s) 183
BpuEI CTTGAG 1 cut(s) 212
Bsa29I ATCGAT 1 cut(s) 216
BseBI CCWGG 1 cut(s) 86
BseCI ATCGAT 1 cut(s) 216
BseXI GCAGC 2 cut(s) 21, 235
BseYI CCCAGC 1 cut(s) 239
BshFI GGCC 2 cut(s) 114, 138
BshVI ATCGAT 1 cut(s) 216
BsnI GGCC 2 cut(s) 114, 138
Bsp119I TTCGAA 1 cut(s) 183
BspACI CCGC 2 cut(s) 71, 264
BspANI GGCC 2 cut(s) 114, 138
BspDI ATCGAT 1 cut(s) 216
BspT104I TTCGAA 1 cut(s) 183
Bst2UI CCWGG 1 cut(s) 86
Bst4CI ACNGT 1 cut(s) 259
BstBI TTCGAA 1 cut(s) 183
BstC8I GCNNGC 1 cut(s) 39
BstMWI GCNNNNNNNGC 1 cut(s) 229
BstNI CCWGG 1 cut(s) 86
BstSCI CCNGG 1 cut(s) 84
BstV1I GCAGC 2 cut(s) 21, 235
Bsu15I ATCGAT 1 cut(s) 216
BsuRI GGCC 2 cut(s) 114, 138
BsuTUI ATCGAT 1 cut(s) 216
BtsIMutI CAGTG 1 cut(s) 26
Cac8I GCNNGC 1 cut(s) 39
ClaI ATCGAT 1 cut(s) 216
Csp6I GTAC 1 cut(s) 44
CviAII CATG 1 cut(s) 102
CviJI RGCY 8 cut(s) 5, 114, 119, 138, 167, 174, 226, 232
CviKI_1 RGCY 8 cut(s) 5, 114, 119, 138, 167, 174, 226, 232
CviQI GTAC 1 cut(s) 44
EaeI YGGCCR 2 cut(s) 112, 136
EcoRII CCWGG 1 cut(s) 84
FaeI CATG 1 cut(s) 105
FaiI YATR 3 cut(s) 59, 103, 221
FatI CATG 1 cut(s) 101
FauI CCCGC 1 cut(s) 64
Fnu4HI GCNGC 2 cut(s) 35, 224
Fsp4HI GCNGC 2 cut(s) 35, 224
GluI GCNGC 2 cut(s) 35, 224
GsaI CCCAGC 1 cut(s) 243
HaeIII GGCC 2 cut(s) 114, 138
Hin1II CATG 1 cut(s) 105
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 1 cut(s) 185
HphI GGTGA 1 cut(s) 253
HpyCH4III ACNGT 1 cut(s) 259
HpyCH4V TGCA 1 cut(s) 223
HpyF10VI GCNNNNNNNGC 1 cut(s) 229
Hsp92II CATG 1 cut(s) 105
LpnPI CCDG 6 cut(s) 71, 98, 156, 162, 225, 237
Lsp1109I GCAGC 2 cut(s) 21, 235
LweI GCATC 1 cut(s) 21
MaeIII GTNAC 2 cut(s) 74, 259
MlsI TGGCCA 1 cut(s) 138
MluCI AATT 1 cut(s) 96
MluNI TGGCCA 1 cut(s) 138
MnlI CCTC 3 cut(s) 57, 76, 178
Mox20I TGGCCA 1 cut(s) 138
MscI TGGCCA 1 cut(s) 138
Msp20I TGGCCA 1 cut(s) 138
MspR9I CCNGG 1 cut(s) 86
MvaI CCWGG 1 cut(s) 86
MwoI GCNNNNNNNGC 1 cut(s) 229
NlaIII CATG 1 cut(s) 105
NmeAIII GCCGAG 1 cut(s) 140
NmuCI GTSAC 1 cut(s) 259
NspV TTCGAA 1 cut(s) 183
PcsI WCGNNNNNNNCGW 1 cut(s) 213
PfeI GAWTC 1 cut(s) 185
PkrI GCNGC 2 cut(s) 36, 225
Psp6I CCWGG 1 cut(s) 84
PspFI CCCAGC 1 cut(s) 239
PspGI CCWGG 1 cut(s) 84
RsaI GTAC 1 cut(s) 45
RsaNI GTAC 1 cut(s) 44
SatI GCNGC 2 cut(s) 35, 224
ScrFI CCNGG 1 cut(s) 86
SetI ASST 3 cut(s) 49, 228, 234
SfaNI GCATC 1 cut(s) 21
SfuI TTCGAA 1 cut(s) 183
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
Sse9I AATT 1 cut(s) 96
SsiI CCGC 2 cut(s) 71, 264
StyD4I CCNGG 1 cut(s) 84
TaaI ACNGT 1 cut(s) 259
TaqI TCGA 3 cut(s) 159, 183, 216
TasI AATT 1 cut(s) 96
TfiI GAWTC 1 cut(s) 185
TscAI CASTG 1 cut(s) 33
TseFI GTSAC 1 cut(s) 259
TseI GCWGC 2 cut(s) 34, 223
Tsp45I GTSAC 1 cut(s) 259
TspDTI ATGAA 1 cut(s) 210
TspRI CASTG 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.