Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0017477

Physical Location & Seq

Genomic Coordinates Forward (+)

11758757 .. 11762365

Gene Structure 3609 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g09780.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2634 bp

ATGCTATCAAGATCTTGCTTTGATTTGTTGAATTTGGACCCCATTGACGATTTCCGGGCCGTCAGTCGGATCCCCAGAGTCATGAAAGCCTCCGGGTTTATCTCCGATTTCGAAAACGCCGACCACCACGGCTCCGGCGGGTCCTCCTCCTCCTCTTCATCTGACAACAAGAACAACAACAACGTTGTTTCCTCTGCGCAACAAGAACAACAACGCAGAATCATTGTCTCCAACCACTTGCCAATACACGCTTTTCGTGACGCAGAAACCAACAAGTGGAGTTTCGAATACGACCACGACAGCTTGGTCCTCCAACTTAAAGACGGCGGCGGCTTTCCCCCCAACGTTGAGGTCCTTTACGTGGGTTGCTTAAAGGGCGCTGAGATCGACCCCTGCGATCAGGACGACGTCGCATTGGTCCTCCTCCACGAGTTTCATTGCGTGCCCTGTTTTCTACCGACGGAAATACTCAACAAGTTTTATCATGGTTTCTGTAAGCACTATCTATGGCCTCTGTTTCACTACATGTTGCCCATGACGCCCAGCCACGGTGCACGTTTCGACCGTGCACTCTGGCAGGCCTACGTGTCAGCCAACAAGGCCTTCGCTGATAGAATGATAGAGGTTCTGAACCCTGATGAGGACTACGTTTGGATCCATGACTACCATCTTATGGTGTTGCCTACTTTCTTGAGGAAAAGGTACTACCGTGTCAAGCTTGGTTTCTTTCTGCACAGTCCTTTTCCATCGTCGGAGATTTACCGGACCATACCGGTCCGGGAAGAGATTCTGAGGGCGCTGCTGAATTGTGATCTTATTGGGTTTCATATATTTGACTATGCAAGGCACTTTTTGTCTTGTTGTAGTAGAATGCTAGGTCTTCACTATGAGTTCAAAAGAGGGTACATGGGTTTGGAGTACTATGGTAGAATAGTGAGTATTAAGATGTTGCCTGTGGGGATTCATATGGGTCAGCTTCAGAGTGTTATGTCACTTGGAGTTACTGCTGAGAAAGTGAAGGCATTGAAGGAAAAGTTTGAGGGGAAGATTGTGATTTTGGGGGTTGATGATATGGACTTGTTTAAGGGAATCAGTTTGAAGTTTCTGGCAATGAAGCAGCTTTTGGAGGAGCATCATAGTTTGAGGGGGAAGGTGGTTTTAGTGCAGATAACAAACCCAGCGCGAAGTGGAGGAAAGGATGTGCAGGAGGTTCTGAGTGAGATGAGTGCTATTGCAAAAGAGATCAATAAGAAGTATGGCCAACCTGGGTATCAGCCTATTGTTGTGATCCATGGACCAGTCACGACGCAAGAAAAAGCTGCGTATTATGCCATTTCGGAGTGCTGTTTGGTTAATGCTGTGAGGGATGGGATGAATTTAGTGCCTTACACGTACTCTGTTTGTAGGCAGGGGAGCTCTGTTTTGCACAAGGCATTGGGGATTGTTGATGAGGCAGCAGTGACGAAAAAGAGTGTGATCATTGTCTCTGAATTTATTGGATGTTCTCCTTCTCTTAGTGGCGCAATCCGTGTAAATCCGTGGAACATTGATGCTGTCTCTGGTGCCATGAATTTTGCCATCACAATGTCGGAGGAAGAGAAGCATTTTCGCCATGACAAGCATTACAAGTATATCAGCTCCCATGATGTTGCTTATTGGGCTCAGAGTTTTGATCAAGATCTTGAGAGAGCTTGCAGAGAGCATTACAGGAGGAGATGCTGGGGAATCGGTTTAGGTTTGGGATTCAGAGTTGTAGCTTTGGGATTCAACTTCAGGAAGCTTTCTGTCGATCACATTGCCTATGCCTACAGAAATACAAGTAGCCGGCTCATCTTATTGGACTATGATGGCACTATGACACCTCAAGCTTCAGTGGATAAAGCTCCAAGCAGTGAAGTTATTTCTGTCTTGAATTGCTTATGCAGTGATCCGAAAAACATTGTTTTCATTGTGAGTGGCAGAGGGAAGAATTCTCTCAGCAAATGGTTTTCGCAGTGTGAGAAACTTGGTTTATCAGCAGAGCATGGTTACTTCACTAGGTGGAGTAAATATTCTCCTTGGGAGACTTGCACCTTGACAATGGATTACAGTTGGAAGAAGATTGTGCTGCCTATTATGGAGTCCTACACAGAAGCAACCGATGGTTCTTTCATAGAACAGAAAGACAGTGCACTGGTTTGGCATCATCAAGATGGTGATCCTCATTTTGGCTCATCTCAGGCCAAGGAGCTACTTGATCACCTAGAGAGTGTACTTGTCAATGAGCCGGTTGTAGTTAAAAGAGGCCAACACATTGTGGAAGTGAAACCACTGGGTGTGAGCAAAGGCAAAGTTGTGGAGGAGCTCCTTTCTACTATGCAAAATAGGGTGAGGTCACCAGATTTCTTGTTATGCATAGGGGATGATCGCTCGGATGAAGACATGTTCAAGAGTATCATGCATCCCGAGTCAAATCCATCCATGCCAGCCATTGCTGAAGTCTTTGCTTGTACTGTTGGTCAAAAACCAAGCATGGCAAAATACTATCTGGATGATACAGTTGATGTCATCCAGTTGCTCCAAGGCGTCGCTTCAGCATCAGCGCAACCGAAGTCTGTACCGCAACATCCGGAATCATTTGAAGTGTTCATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005975 GO:0005984 GO:0005991 GO:0005992 GO:0006793 GO:0006796 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009311 GO:0009312 GO:0009987 GO:0016051 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0033554 GO:0034637 GO:0042578 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044262 GO:0044424 GO:0044444 GO:0044464 GO:0046351 GO:0050896 GO:0051716 GO:0070413 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glyco_transf_20 Trehalose_PPase

Protein Analysis

878

Amino Acids

99.04

Weight (kDa)

6.42

Isoelectric Point (pI)

51.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_20	PF00982	72 - 562	2.1e-175	Glycosyltransferase family 20
Trehalose_PPase	PF02358	612 - 846	1.1e-82	Trehalose-phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017477)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G18700
fragaria_vesca	FvH4_4g09780
prunus_persica	Prupe.1G095500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0404641
rosa_laevigata	RLG00000008917
rosa_multiflora	Rmu_sc0002699.1_g000030
rosa_roxburghii	Rroxscaffold_5G00348790
rosa_rugosa	Rorug04G0048300
rosa_samantha	Rh4AG124100 Rh4BG118100 Rh4CG132200
rosa_wichuraiana	Rw4G010060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	305
AatII	GACGTC	1 cut(s)	411
Acc16I	TGCGCA	1 cut(s)	198
AccB1I	GGYRCC	1 cut(s)	1562
AccB7I	CCANNNNNTGG	2 cut(s)	276, 673
AccII	CGCG	1 cut(s)	1183
AccIII	TCCGGA	1 cut(s)	2608
AciI	CCGC	4 cut(s)	138, 327, 330, 2600
AclI	AACGTT	2 cut(s)	183, 345
AclWI	GGATC	7 cut(s)	64, 77, 649, 662, 1282, 1922, 2192
AcoI	YGGCCR	1 cut(s)	1258
AcsI	RAATTY	5 cut(s)	31, 1375, 1490, 1570, 1969
AcuI	CTGAAG	5 cut(s)	962, 1756, 1854, 2496, 2556
AcyI	GRCGYC	3 cut(s)	408, 539, 2565
AdeI	CACNNNGTG	3 cut(s)	2040, 2296, 2315
AfaI	GTAC	7 cut(s)	704, 905, 920, 1394, 2253, 2491, 2598
AfiI	CCNNNNNNNGG	7 cut(s)	66, 276, 361, 548, 640, 673, 2207
AflIII	ACRYGT	4 cut(s)	525, 585, 1389, 2421
AgeI	ACCGGT	1 cut(s)	772
AgsI	TTSAA	8 cut(s)	31, 895, 1027, 1099, 1768, 1912, 2428, 2621
AjnI	CCWGG	1 cut(s)	1264
AjuI	GAANNNNNNNTTGG	4 cut(s)	587, 619, 1106, 1138
Alw21I	GWGCWC	5 cut(s)	556, 571, 1418, 2173, 2346
Alw26I	GTCTC	4 cut(s)	232, 1489, 1561, 2057
Alw44I	GTGCAC	3 cut(s)	552, 567, 2169
AlwI	GGATC	7 cut(s)	64, 77, 649, 662, 1282, 1922, 2192
Ama87I	CYCGRG	1 cut(s)	2444
Aor13HI	TCCGGA	1 cut(s)	2608
AoxI	GGCC	7 cut(s)	57, 509, 579, 600, 1258, 2220, 2284
ApaLI	GTGCAC	3 cut(s)	552, 567, 2169
ApeKI	GCWGC	5 cut(s)	799, 1117, 1319, 1454, 2107
ApoI	RAATTY	5 cut(s)	31, 1375, 1490, 1570, 1969
AsiGI	ACCGGT	1 cut(s)	772
Asp700I	GAANNNNTTC	1 cut(s)	786
AspLEI	GCGC	6 cut(s)	199, 380, 799, 1183, 1523, 2584
AspS9I	GGNCC	9 cut(s)	37, 57, 141, 307, 352, 418, 765, 775, 1295
AsuC2I	CCSGG	3 cut(s)	56, 94, 779
AsuHPI	GGTGA	4 cut(s)	2207, 2231, 2367, 2380
AsuII	TTCGAA	2 cut(s)	111, 285
AvaI	CYCGRG	1 cut(s)	2444
AvaII	GGWCC	8 cut(s)	37, 141, 307, 352, 418, 765, 775, 1295
BaeGI	GKGCMC	4 cut(s)	447, 556, 571, 2173
BaeI	ACNNNNGTAYC	6 cut(s)	694, 727, 895, 928, 1253, 1286
BalI	TGGCCA	1 cut(s)	1260
BamHI	GGATCC	2 cut(s)	69, 654
BanI	GGYRCC	1 cut(s)	1562
BanII	GRGCYC	3 cut(s)	1418, 1663, 2346
BauI	CACGAG	1 cut(s)	428
BbsI	GAAGAC	2 cut(s)	872, 2424
Bbv12I	GWGCWC	5 cut(s)	556, 571, 1418, 2173, 2346
BbvI	GCAGC	5 cut(s)	786, 1129, 1306, 1466, 2094
BccI	CCATC	8 cut(s)	675, 754, 1361, 1586, 1841, 2135, 2186, 2464
BceAI	ACGGC	3 cut(s)	44, 145, 340
BcgI	CGANNNNNNTGC	2 cut(s)	1778, 1812
BciT130I	CCWGG	1 cut(s)	1266
BclI	TGATCA	3 cut(s)	1476, 1672, 2236
BcnI	CCSGG	3 cut(s)	56, 94, 779
BcoDI	GTCTC	4 cut(s)	232, 1489, 1561, 2057
BfaI	CTAG	3 cut(s)	875, 2037, 2243
BfmI	CTRYAG	1 cut(s)	1807
BfoI	RGCGCY	2 cut(s)	381, 800
BglI	GCCNNNNNGGC	1 cut(s)	599
BglII	AGATCT	2 cut(s)	11, 1678
BisI	GCNGC	7 cut(s)	328, 331, 800, 1118, 1320, 1455, 2108
BlsI	GCNGC	7 cut(s)	329, 332, 801, 1119, 1321, 1456, 2109
BmcAI	AGTACT	1 cut(s)	920
Bme1390I	CCNGG	4 cut(s)	56, 94, 779, 1266
Bme18I	GGWCC	8 cut(s)	37, 141, 307, 352, 418, 765, 775, 1295
BmeT110I	CYCGRG	1 cut(s)	2444
BmgT120I	GGNCC	9 cut(s)	37, 57, 141, 307, 352, 418, 765, 775, 1295
BmiI	GGNNCC	6 cut(s)	39, 71, 133, 142, 656, 1564
BmrFI	CCNGG	4 cut(s)	56, 94, 779, 1266
BmrI	ACTGGG	1 cut(s)	2321
BmsI	GCATC	6 cut(s)	1141, 1540, 1706, 2191, 2449, 2585
BmuI	ACTGGG	1 cut(s)	2321
BpiI	GAAGAC	2 cut(s)	872, 2424
Bpu14I	TTCGAA	2 cut(s)	111, 285
BpuEI	CTTGAG	3 cut(s)	712, 1703, 1848
BpuMI	CCSGG	3 cut(s)	56, 94, 779
BsaAI	YACGTR	3 cut(s)	361, 586, 1392
BsaBI	GATNNNNATC	2 cut(s)	1677, 2196
BsaHI	GRCGYC	3 cut(s)	408, 539, 2565
BsaJI	CCNNGG	8 cut(s)	127, 547, 1265, 1291, 1538, 2057, 2223, 2560
BsaWI	WCCGGW	3 cut(s)	762, 772, 2608
BsaXI	ACNNNNNCTCC	2 cut(s)	116, 146
Bsc4I	CCNNNNNNNGG	7 cut(s)	66, 276, 361, 548, 640, 673, 2207
Bse118I	RCCGGY	3 cut(s)	772, 1824, 2266
Bse1I	ACTGG	4 cut(s)	1298, 2178, 2316, 2551
Bse3DI	GCAATG	4 cut(s)	436, 1116, 1794, 2469
Bse8I	GATNNNNATC	2 cut(s)	1677, 2196
BseAI	TCCGGA	1 cut(s)	2608
BseBI	CCWGG	1 cut(s)	1266
BseDI	CCNNGG	8 cut(s)	127, 547, 1265, 1291, 1538, 2057, 2223, 2560
BseJI	GATNNNNATC	2 cut(s)	1677, 2196
BseLI	CCNNNNNNNGG	7 cut(s)	66, 276, 361, 548, 640, 673, 2207
BseMI	GCAATG	4 cut(s)	436, 1116, 1794, 2469
BseMII	CTCAG	7 cut(s)	372, 782, 999, 1205, 1676, 1990, 2231
BseNI	ACTGG	4 cut(s)	1298, 2178, 2316, 2551
BseRI	GAGGAG	7 cut(s)	136, 139, 142, 413, 1142, 1726, 2354
BseSI	GKGCMC	4 cut(s)	447, 556, 571, 2173
BseXI	GCAGC	5 cut(s)	786, 1129, 1306, 1466, 2094
BseYI	CCCAGC	3 cut(s)	542, 1177, 1719
BsgI	GTGCAG	3 cut(s)	716, 1184, 1223
Bsh1236I	CGCG	1 cut(s)	1183
Bsh1285I	CGRYCG	1 cut(s)	565
BshFI	GGCC	7 cut(s)	59, 511, 581, 602, 1260, 2222, 2286
BshNI	GGYRCC	1 cut(s)	1562
BshTI	ACCGGT	1 cut(s)	772
BsiEI	CGRYCG	1 cut(s)	565
BsiHKAI	GWGCWC	5 cut(s)	556, 571, 1418, 2173, 2346
BsiHKCI	CYCGRG	1 cut(s)	2444
BsiSI	CCGG	9 cut(s)	55, 93, 135, 763, 773, 778, 1825, 2267, 2609
BslI	CCNNNNNNNGG	7 cut(s)	66, 276, 361, 548, 640, 673, 2207
BsmAI	GTCTC	4 cut(s)	232, 1489, 1561, 2057
BsmI	GAATGC	1 cut(s)	876
BsnI	GGCC	7 cut(s)	59, 511, 581, 602, 1260, 2222, 2286
BsoBI	CYCGRG	1 cut(s)	2444
Bsp119I	TTCGAA	2 cut(s)	111, 285
Bsp1286I	GDGCHC	7 cut(s)	447, 556, 571, 1418, 1663, 2173, 2346
Bsp13I	TCCGGA	1 cut(s)	2608
Bsp19I	CCATGG	1 cut(s)	1291
BspACI	CCGC	4 cut(s)	138, 327, 330, 2600
BspANI	GGCC	7 cut(s)	59, 511, 581, 602, 1260, 2222, 2286
BspCNI	CTCAG	7 cut(s)	373, 783, 1000, 1206, 1675, 1989, 2230
BspEI	TCCGGA	1 cut(s)	2608
BspFNI	CGCG	1 cut(s)	1183
BspHI	TCATGA	1 cut(s)	81
BspLI	GGNNCC	6 cut(s)	39, 71, 133, 142, 656, 1564
BspPI	GGATC	7 cut(s)	64, 77, 649, 662, 1282, 1922, 2192
BspT104I	TTCGAA	2 cut(s)	111, 285
BspT107I	GGYRCC	1 cut(s)	1562
BsrDI	GCAATG	4 cut(s)	436, 1116, 1794, 2469
BsrFI	RCCGGY	3 cut(s)	772, 1824, 2266
BsrI	ACTGG	4 cut(s)	1298, 2178, 2316, 2551
BssAI	RCCGGY	3 cut(s)	772, 1824, 2266
BssECI	CCNNGG	8 cut(s)	127, 547, 1265, 1291, 1538, 2057, 2223, 2560
BssNI	GRCGYC	3 cut(s)	408, 539, 2565
BssSI	CACGAG	1 cut(s)	428
BssT1I	CCWWGG	4 cut(s)	1291, 2057, 2223, 2560
Bst2BI	CACGAG	1 cut(s)	428
Bst2UI	CCWGG	1 cut(s)	1266
Bst4CI	ACNGT	8 cut(s)	551, 566, 710, 737, 2090, 2168, 2494, 2539
Bst6I	CTCTTC	3 cut(s)	160, 777, 1590
BstACI	GRCGYC	3 cut(s)	408, 539, 2565
BstBAI	YACGTR	3 cut(s)	361, 586, 1392
BstBI	TTCGAA	2 cut(s)	111, 285
BstC8I	GCNNGC	5 cut(s)	443, 579, 1693, 1826, 2466
BstDEI	CTNAG	8 cut(s)	381, 791, 1008, 1214, 1514, 1662, 1976, 2217
BstDSI	CCRYGG	4 cut(s)	127, 547, 1291, 1538
BstEII	GGTNACC	1 cut(s)	2373
BstFNI	CGCG	1 cut(s)	1183
BstH2I	RGCGCY	2 cut(s)	381, 800
BstHHI	GCGC	6 cut(s)	199, 380, 799, 1183, 1523, 2584
BstMAI	GTCTC	4 cut(s)	232, 1489, 1561, 2057
BstMCI	CGRYCG	1 cut(s)	565
BstMWI	GCNNNNNNNGC	5 cut(s)	375, 538, 599, 1328, 1658
BstNI	CCWGG	1 cut(s)	1266
BstNSI	RCATGY	2 cut(s)	529, 2425
BstPI	GGTNACC	1 cut(s)	2373
BstSCI	CCNGG	4 cut(s)	54, 92, 777, 1264
BstSFI	CTRYAG	1 cut(s)	1807
BstSLI	GKGCMC	4 cut(s)	447, 556, 571, 2173
BstUI	CGCG	1 cut(s)	1183
BstV1I	GCAGC	5 cut(s)	786, 1129, 1306, 1466, 2094
BstV2I	GAAGAC	2 cut(s)	872, 2424
BstX2I	RGATCY	4 cut(s)	11, 69, 654, 1678
BstYI	RGATCY	4 cut(s)	11, 69, 654, 1678
BsuRI	GGCC	7 cut(s)	59, 511, 581, 602, 1260, 2222, 2286
BtgI	CCRYGG	4 cut(s)	127, 547, 1291, 1538
BtsI	GCAGTG	4 cut(s)	1464, 1897, 1930, 2000
BtsIMutI	CAGTG	8 cut(s)	1464, 1878, 1897, 1930, 2000, 2171, 2173, 2309
Cac8I	GCNNGC	5 cut(s)	443, 579, 1693, 1826, 2466
CciI	TCATGA	1 cut(s)	81
CfoI	GCGC	6 cut(s)	199, 380, 799, 1183, 1523, 2584
Cfr10I	RCCGGY	3 cut(s)	772, 1824, 2266
Cfr13I	GGNCC	9 cut(s)	37, 57, 141, 307, 352, 418, 765, 775, 1295
CpoI	CGGWCCG	1 cut(s)	775
CseI	GACGC	4 cut(s)	269, 547, 1315, 2554
Csp6I	GTAC	7 cut(s)	703, 904, 919, 1393, 2252, 2490, 2597
CspAI	ACCGGT	1 cut(s)	772
CspI	CGGWCCG	1 cut(s)	775
CviQI	GTAC	7 cut(s)	703, 904, 919, 1393, 2252, 2490, 2597
DdeI	CTNAG	8 cut(s)	381, 791, 1008, 1214, 1514, 1662, 1976, 2217
DraIII	CACNNNGTG	3 cut(s)	2040, 2296, 2315
DrdI	GACNNNNNNGTC	1 cut(s)	305
DseDI	GACNNNNNNGTC	1 cut(s)	305
EaeI	YGGCCR	1 cut(s)	1258
Eam1104I	CTCTTC	3 cut(s)	160, 777, 1590
EarI	CTCTTC	3 cut(s)	160, 777, 1590
Ecl136II	GAGCTC	2 cut(s)	1416, 2344
Eco130I	CCWWGG	4 cut(s)	1291, 2057, 2223, 2560
Eco147I	AGGCCT	2 cut(s)	581, 602
Eco24I	GRGCYC	3 cut(s)	1418, 1663, 2346
Eco47I	GGWCC	8 cut(s)	37, 141, 307, 352, 418, 765, 775, 1295
Eco53kI	GAGCTC	2 cut(s)	1416, 2344
Eco57I	CTGAAG	5 cut(s)	962, 1756, 1854, 2496, 2556
Eco88I	CYCGRG	1 cut(s)	2444
Eco91I	GGTNACC	1 cut(s)	2373
EcoICRI	GAGCTC	2 cut(s)	1416, 2344
EcoO109I	RGGNCCY	2 cut(s)	141, 352
EcoO65I	GGTNACC	1 cut(s)	2373
EcoRI	GAATTC	1 cut(s)	1969
EcoRII	CCWGG	1 cut(s)	1264
EcoT14I	CCWWGG	4 cut(s)	1291, 2057, 2223, 2560
EcoT22I	ATGCAT	2 cut(s)	2396, 2442
EcoT38I	GRGCYC	3 cut(s)	1418, 1663, 2346
ErhI	CCWWGG	4 cut(s)	1291, 2057, 2223, 2560
FauI	CCCGC	1 cut(s)	131
FauNDI	CATATG	1 cut(s)	966
FbaI	TGATCA	3 cut(s)	1476, 1672, 2236
Fnu4HI	GCNGC	7 cut(s)	328, 331, 800, 1118, 1320, 1455, 2108
FriOI	GRGCYC	3 cut(s)	1418, 1663, 2346
Fsp4HI	GCNGC	7 cut(s)	328, 331, 800, 1118, 1320, 1455, 2108
FspBI	CTAG	3 cut(s)	875, 2037, 2243
FspI	TGCGCA	1 cut(s)	198
GlaI	GCGC	6 cut(s)	198, 379, 798, 1182, 1522, 2583
GluI	GCNGC	7 cut(s)	328, 331, 800, 1118, 1320, 1455, 2108
GsaI	CCCAGC	3 cut(s)	546, 1181, 1723
HaeII	RGCGCY	2 cut(s)	381, 800
HaeIII	GGCC	7 cut(s)	59, 511, 581, 602, 1260, 2222, 2286
HapII	CCGG	9 cut(s)	55, 93, 135, 763, 773, 778, 1825, 2267, 2609
HgaI	GACGC	4 cut(s)	269, 547, 1315, 2554
HhaI	GCGC	6 cut(s)	199, 380, 799, 1183, 1523, 2584
Hin1I	GRCGYC	3 cut(s)	408, 539, 2565
Hin6I	GCGC	6 cut(s)	197, 378, 797, 1181, 1521, 2582
HinP1I	GCGC	6 cut(s)	197, 378, 797, 1181, 1521, 2582
HindIII	AAGCTT	3 cut(s)	716, 1778, 1866
HpaII	CCGG	9 cut(s)	55, 93, 135, 763, 773, 778, 1825, 2267, 2609
HphI	GGTGA	4 cut(s)	2207, 2231, 2367, 2380
Hpy166II	GTNNAC	4 cut(s)	554, 569, 2171, 2252
Hpy8I	GTNNAC	4 cut(s)	554, 569, 2171, 2252
Hpy99I	CGWCG	6 cut(s)	410, 413, 463, 754, 1309, 2570
HpyAV	CCTTC	5 cut(s)	613, 1012, 1021, 1144, 1518
HpyCH4III	ACNGT	8 cut(s)	551, 566, 710, 737, 2090, 2168, 2494, 2539
HpyCH4IV	ACGT	8 cut(s)	183, 345, 360, 408, 556, 585, 648, 1391
HpyF10VI	GCNNNNNNNGC	5 cut(s)	375, 538, 599, 1328, 1658
HpyF3I	CTNAG	8 cut(s)	381, 791, 1008, 1214, 1514, 1662, 1976, 2217
HpySE526I	ACGT	8 cut(s)	183, 345, 360, 408, 556, 585, 648, 1391
Hsp92I	GRCGYC	3 cut(s)	408, 539, 2565
HspAI	GCGC	6 cut(s)	197, 378, 797, 1181, 1521, 2582
Kpn2I	TCCGGA	1 cut(s)	2608
KroI	GCCGGC	1 cut(s)	1824
KroNI	GCCGGC	1 cut(s)	1826
Ksp22I	TGATCA	3 cut(s)	1476, 1672, 2236
LmnI	GCTCC	9 cut(s)	137, 1129, 1413, 1643, 1888, 2227, 2341, 2349, 2562
Lsp1109I	GCAGC	5 cut(s)	786, 1129, 1306, 1466, 2094
LweI	GCATC	6 cut(s)	1141, 1540, 1706, 2191, 2449, 2585
MaeI	CTAG	3 cut(s)	875, 2037, 2243
MaeII	ACGT	8 cut(s)	183, 345, 360, 408, 556, 585, 648, 1391
MaeIII	GTNAC	7 cut(s)	257, 990, 1000, 1300, 1459, 2027, 2373
MboII	GAAGA	9 cut(s)	147, 794, 872, 1057, 1607, 1978, 2107, 2110, 2429
MflI	RGATCY	4 cut(s)	11, 69, 654, 1678
MhlI	GDGCHC	7 cut(s)	447, 556, 571, 1418, 1663, 2173, 2346
MlsI	TGGCCA	1 cut(s)	1260
MluCI	AATT	7 cut(s)	31, 805, 1375, 1490, 1570, 1912, 1969
MluNI	TGGCCA	1 cut(s)	1260
MlyI	GAGTC	3 cut(s)	87, 2129, 2456
MmeI	TCCRAC	6 cut(s)	47, 255, 337, 732, 1569, 2072
Mox20I	TGGCCA	1 cut(s)	1260
Mph1103I	ATGCAT	2 cut(s)	2396, 2442
MroI	TCCGGA	1 cut(s)	2608
MroNI	GCCGGC	1 cut(s)	1824
MroXI	GAANNNNTTC	1 cut(s)	786
MscI	TGGCCA	1 cut(s)	1260
MseI	TTAA	6 cut(s)	318, 371, 942, 1083, 1353, 2277
MslI	CAYNNNNRTG	2 cut(s)	1583, 2190
Msp20I	TGGCCA	1 cut(s)	1260
MspI	CCGG	9 cut(s)	55, 93, 135, 763, 773, 778, 1825, 2267, 2609
MspR9I	CCNGG	4 cut(s)	56, 94, 779, 1266
Mva1269I	GAATGC	1 cut(s)	876
MvaI	CCWGG	1 cut(s)	1266
MvnI	CGCG	1 cut(s)	1183
MwoI	GCNNNNNNNGC	5 cut(s)	375, 538, 599, 1328, 1658
NaeI	GCCGGC	1 cut(s)	1826
NciI	CCSGG	3 cut(s)	56, 94, 779
NcoI	CCATGG	1 cut(s)	1291
NdeI	CATATG	1 cut(s)	966
NgoMIV	GCCGGC	1 cut(s)	1824
NlaIV	GGNNCC	6 cut(s)	39, 71, 133, 142, 656, 1564
NmuCI	GTSAC	5 cut(s)	257, 990, 1300, 1459, 2373
NsbI	TGCGCA	1 cut(s)	198
NsiI	ATGCAT	2 cut(s)	2396, 2442
NspI	RCATGY	2 cut(s)	529, 2425
NspV	TTCGAA	2 cut(s)	111, 285
PagI	TCATGA	1 cut(s)	81
PceI	AGGCCT	2 cut(s)	581, 602
PciI	ACATGT	2 cut(s)	525, 2421
PcsI	WCGNNNNNNNCGW	3 cut(s)	117, 393, 562
PctI	GAATGC	1 cut(s)	876
PdiI	GCCGGC	1 cut(s)	1826
PdmI	GAANNNNTTC	1 cut(s)	786
PfeI	GAWTC	8 cut(s)	219, 787, 961, 1089, 1725, 1743, 1764, 2612
PflFI	GACNNNGTC	1 cut(s)	407
PflMI	CCANNNNNTGG	2 cut(s)	276, 673
PfoI	TCCNGGA	1 cut(s)	777
PinAI	ACCGGT	1 cut(s)	772
PkrI	GCNGC	7 cut(s)	329, 332, 801, 1119, 1321, 1456, 2109
PleI	GAGTC	3 cut(s)	86, 2128, 2455
PpsI	GAGTC	3 cut(s)	86, 2128, 2455
Ppu21I	YACGTR	3 cut(s)	361, 586, 1392
PpuMI	RGGWCCY	2 cut(s)	141, 352
PscI	ACATGT	2 cut(s)	525, 2421
Psp124BI	GAGCTC	2 cut(s)	1418, 2346
Psp1406I	AACGTT	2 cut(s)	183, 345
Psp5II	RGGWCCY	2 cut(s)	141, 352
Psp6I	CCWGG	1 cut(s)	1264
PspEI	GGTNACC	1 cut(s)	2373
PspFI	CCCAGC	3 cut(s)	542, 1177, 1719
PspGI	CCWGG	1 cut(s)	1264
PspN4I	GGNNCC	6 cut(s)	39, 71, 133, 142, 656, 1564
PspPI	GGNCC	9 cut(s)	37, 57, 141, 307, 352, 418, 765, 775, 1295
PspPPI	RGGWCCY	2 cut(s)	141, 352
PsuI	RGATCY	4 cut(s)	11, 69, 654, 1678
PsyI	GACNNNGTC	1 cut(s)	407
RsaI	GTAC	7 cut(s)	704, 905, 920, 1394, 2253, 2491, 2598
RsaNI	GTAC	7 cut(s)	703, 904, 919, 1393, 2252, 2490, 2597
RseI	CAYNNNNRTG	2 cut(s)	1583, 2190
Rsr2I	CGGWCCG	1 cut(s)	775
RsrII	CGGWCCG	1 cut(s)	775
SacI	GAGCTC	2 cut(s)	1418, 2346
SaqAI	TTAA	6 cut(s)	318, 371, 942, 1083, 1353, 2277
SatI	GCNGC	7 cut(s)	328, 331, 800, 1118, 1320, 1455, 2108
Sau96I	GGNCC	9 cut(s)	37, 57, 141, 307, 352, 418, 765, 775, 1295
ScaI	AGTACT	1 cut(s)	920
SchI	GAGTC	3 cut(s)	87, 2129, 2456
ScrFI	CCNGG	4 cut(s)	56, 94, 779, 1266
SduI	GDGCHC	7 cut(s)	447, 556, 571, 1418, 1663, 2173, 2346
SfaNI	GCATC	6 cut(s)	1141, 1540, 1706, 2191, 2449, 2585
SfcI	CTRYAG	1 cut(s)	1807
SfuI	TTCGAA	2 cut(s)	111, 285
SinI	GGWCC	8 cut(s)	37, 141, 307, 352, 418, 765, 775, 1295
SmiMI	CAYNNNNRTG	2 cut(s)	1583, 2190
SmlI	CTYRAG	3 cut(s)	691, 1682, 1863
SmoI	CTYRAG	3 cut(s)	691, 1682, 1863
Sse9I	AATT	7 cut(s)	31, 805, 1375, 1490, 1570, 1912, 1969
SseBI	AGGCCT	2 cut(s)	581, 602
SsiI	CCGC	4 cut(s)	138, 327, 330, 2600
SspI	AATATT	1 cut(s)	2051
SspMI	CTAG	3 cut(s)	875, 2037, 2243
SstI	GAGCTC	2 cut(s)	1418, 2346
StuI	AGGCCT	2 cut(s)	581, 602
StyD4I	CCNGG	4 cut(s)	54, 92, 777, 1264
StyI	CCWWGG	4 cut(s)	1291, 2057, 2223, 2560
TaaI	ACNGT	8 cut(s)	551, 566, 710, 737, 2090, 2168, 2494, 2539
TaiI	ACGT	8 cut(s)	186, 348, 363, 411, 559, 588, 651, 1394
TaqI	TCGA	5 cut(s)	111, 285, 387, 561, 1788
TasI	AATT	7 cut(s)	31, 805, 1375, 1490, 1570, 1912, 1969
TatI	WGTACW	3 cut(s)	918, 2251, 2489
TauI	GCSGC	2 cut(s)	330, 333
TfiI	GAWTC	8 cut(s)	219, 787, 961, 1089, 1725, 1743, 1764, 2612
Tru1I	TTAA	6 cut(s)	318, 371, 942, 1083, 1353, 2277
Tru9I	TTAA	6 cut(s)	318, 371, 942, 1083, 1353, 2277
TscAI	CASTG	8 cut(s)	1464, 1878, 1897, 1930, 2000, 2173, 2178, 2316
TseFI	GTSAC	5 cut(s)	257, 990, 1300, 1459, 2373
TseI	GCWGC	5 cut(s)	799, 1117, 1319, 1454, 2107
Tsp45I	GTSAC	5 cut(s)	257, 990, 1300, 1459, 2373
TspGWI	ACGGA	3 cut(s)	476, 1517, 1527
TspRI	CASTG	8 cut(s)	1464, 1878, 1897, 1930, 2000, 2173, 2178, 2316
Tth111I	GACNNNGTC	1 cut(s)	407
Van91I	CCANNNNNTGG	2 cut(s)	276, 673
VneI	GTGCAC	3 cut(s)	552, 567, 2169
VpaK11BI	GGWCC	8 cut(s)	37, 141, 307, 352, 418, 765, 775, 1295
XapI	RAATTY	5 cut(s)	31, 1375, 1490, 1570, 1969
XceI	RCATGY	2 cut(s)	529, 2425
XmnI	GAANNNNTTC	1 cut(s)	786
XspI	CTAG	3 cut(s)	875, 2037, 2243
ZraI	GACGTC	1 cut(s)	409
ZrmI	AGTACT	1 cut(s)	920
Zsp2I	ATGCAT	2 cut(s)	2396, 2442

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.