Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0017477

Physical Location & Seq

Genomic Coordinates Reverse (-)

19819275 .. 19822297

Gene Structure 3023 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG118100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2634 bp

ATGCTCTCGAGATCTTGCTTTGATTTGCTGAATTTGGACCCCATTGACGATTTCCGGGCCGTCAGTCGGATCCCCAGAGTCATGCAAGCCCCCGGCTTCATCTCCGAGTTCGACAACAACTCCGACGACCGTTCCGGCGGCTCATCCTCATCCGACAACAACAACAACAACAACGTTGTTTCCTCTGTTTCGCAGGAACGAAGAATCATCGTCTCCAACCACTTGCCAATACGCGCGTTTCGAGACGAAGACGAAACCAACAAGTGGCGTTTCGAATACGACCACGACAGCTTGGTCCTCCAACTCAAAGACGGCGGCGGCTTTGGCGCGGACGTCGAGGTCTTGTACGTGGGTTGCTTAAACGGAGTCGAGATCGACCCCTGCGATCAAGACGACGTCGCATTTGTCCTCCTCCACGAGTTCCGCTGCGTGCCGTGTTTTCTACCGACGGAAGTCGTCAACAAGTTTTACCACGGCTTCTGTAAGCACTATCTTTGGCCTCTGTTTCACTACATGCTGCCCATGTCAGCCAGCCACGGTGCACGCTTCGACCGTACACTCTGGCAGGCCTACGTGTCGGCCAACAAGGCCTTCGCCGATAGAATCATAGAGGTTCTAAACCCGGATGAGGACTACGTTTGGATCCATGACTACCATCTCATGGTTTTGCCTACTTTCTTGAGGAAAAGGTACTACCGGGTCAAGCTTGGTTTCTTTCTGCATAGTCCTTTTCCGTCGTCGGAGATTTACCGGACGATTCCGGTCCGGGAGGAGATTCTGAGGGCTCTGTTGAATTGTGATCTTATTGGGTTCCATATATTTGATTATGCGAGGCACTTCTTGTCCTGCTGTAGCAGAATGCTGGGCCTTCATTATGAGTTCAAAAGAGGGTACATGGGTTTGGAGTATTATGGTCGAATAGTGAGTATTAAGATGTTGCCTGTGGGGATTCACATGGGTCAGCTTCAGTCTGTTTTGTCGCTTGATGATACTGCCAAGAAAGTGAAGGTGTTGAAGGAAAAGTTTGAGGGGAAGATTGTGATTTTGGGGGTGGATGATTTGGACTTGTTTAAGGGGATTAGTTTGAAGTTTCTGGCAATGAAGCAGCTTTTGGAAGAGCATCAGAGTTTGAGGGGGAAGGTGGTTATGGTGCAGATAACAAACCCGGCGCGAAGCCGAGGCAAGGATGTGCAGGAGGTTCTGAATGAGACCAGTGCTATTGCTAAAGAGATCAATGACAAGTATGGCCGACCCGGGTACCAGCCCCTTGTTGTCATCGATGGACCTGTGACGACGCAAGACAAAGCTGCATATTATGCCATTTCGGAGTGCTGTTTGGTTAATGCTGTGAGGGATGGGATGAATTTGGTGCCTTACACGTACACTGTTTGTAGGCAGGGGAGCTCTGTTTTGGACAAGGTGTTGGAGATTGGTGATGAGGCAGCAGATACGACACCTAAACCGAAAAAGAGTGTGATCATTGTCTCTGAATTTATTGGCTGTTCTCCGTCTCTTAGTGGCGCAATCCGTGTCAATCCCTGGAACATTGATGCTGTCTCTGATGCCATGAATTTGGTCATCACAATGTCGGAGGAAGAGAAGCGTCTTCGCCATGACAAACATTACAAGTATATCAGCTCTCATGATGTTGCTTATTGGGCTCGGAGTTTTGATCAAGATCTTGAGAGAGCTTGCAGAGAGCATTATCGGAGGAGATGCTGGGGCATTGGTTTAGGTTTGGGATTCAGAGTTGTAGCTTTGGGACCCAACTTCAGGAAGCTTTCTGTGGATCACATTGCCTATGCTTACAGAAATACAAGTAGCCGGCTCATCTTATTGGACTATGACGGCACTATGACACCTCAGGCTTCAGTGGACAAAGCTCCAAGCAGTGAAGTTATTTCGGTCTTGAATTGCTTATGCAGTGATCCGAAAAACATTGTTTTCATTGTGAGTGGCAGAGGGAAGGATTCTCTCAGCAAGTGGTTTTCGCAGTGTGAGAAACTAGGTTTATCAGCAGAGCATGGTTACTTCACCAGGTGGACTAAAGATTCTCCTTGGAAGACTTGCTCCTTGACAATGGATTTCAGTTGGAAGAAGATTGTGCTGCCTATTATGGAGCCTTACACAGAAGCAACCGATGGTTCTTTTATAGAGGAGAAAGACAGTGCACTGGTCTGGCATCATCAAGATGCTGATCCTCATTTCGGCTCATCCCAGGCTAAAGAGCTGCTTGATCACCTAGAGAGTGTGCTTGTCAATGAGCCGGTTGTAGTTAAAAGAGGCCAACACATTGTGGAAGTGAAACCACTGGATGTGAACAAAGGTATAGTTGTGGAGGAGCTCATCTCCACTATGCGAAATAGGGTGAAGTCACCAGATTTCTTGTTATGCATAGGGGATGATCGCTCAGATGAGGATATGTTTAAGATCATCATGTATCCCGAATCAAGTCCGTCCTTGCCAGCCATTGCAGAAGTCTTTGCTTGCAGTGTTGGTCAAAAACCAAGCATGGCAAAATACTATCTGGATGATACAGTTGATGTCATCAAGTTGCTTCAAGGCCTCGCTTCAGAATCAGCGCAACCAAAGTCTGGACCGCAGTTTCCGGAAGCATTTGAAGTGTTCATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005975 GO:0005984 GO:0005991 GO:0005992 GO:0006793 GO:0006796 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009311 GO:0009312 GO:0009987 GO:0016051 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0033554 GO:0034637 GO:0042578 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044262 GO:0044424 GO:0044444 GO:0044464 GO:0046351 GO:0050896 GO:0051716 GO:0070413 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glyco_transf_20 Trehalose_PPase

Protein Analysis

877

Amino Acids

99.48

Weight (kDa)

5.89

Isoelectric Point (pI)

49.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_20	PF00982	67 - 562	9.9e-176	Glycosyltransferase family 20
Trehalose_PPase	PF02358	612 - 846	2.2e-80	Trehalose-phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017477)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G18700
fragaria_vesca	FvH4_4g09780
prunus_persica	Prupe.1G095500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0404641
rosa_laevigata	RLG00000008917
rosa_multiflora	Rmu_sc0002699.1_g000030
rosa_roxburghii	Rroxscaffold_5G00348790
rosa_rugosa	Rorug04G0048300
rosa_samantha	Rh4AG124100 Rh4BG118100 Rh4CG132200
rosa_wichuraiana	Rw4G010060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	293, 338
AatII	GACGTC	2 cut(s)	336, 399
Acc65I	GGTACC	1 cut(s)	1257
AccB1I	GGYRCC	2 cut(s)	1257, 1369
AccB7I	CCANNNNNTGG	3 cut(s)	264, 661, 2594
AccII	CGCG	4 cut(s)	234, 236, 329, 1171
AccIII	TCCGGA	1 cut(s)	2608
AciI	CCGC	6 cut(s)	138, 315, 318, 329, 424, 2600
AclI	AACGTT	1 cut(s)	174
AclWI	GGATC	7 cut(s)	64, 77, 637, 650, 1797, 1922, 2192
AcoI	YGGCCR	2 cut(s)	579, 1246
AcsI	RAATTY	4 cut(s)	31, 1363, 1490, 1570
AcuI	CTGAAG	4 cut(s)	950, 1756, 1854, 2556
AcyI	GRCGYC	2 cut(s)	333, 396
AdeI	CACNNNGTG	2 cut(s)	2040, 2296
AfaI	GTAC	6 cut(s)	347, 556, 692, 893, 1259, 1382
AfiI	CCNNNNNNNGG	8 cut(s)	66, 264, 628, 661, 1183, 1773, 2207, 2594
AflIII	ACRYGT	2 cut(s)	573, 1377
AgsI	TTSAA	7 cut(s)	793, 883, 1015, 1087, 1912, 2561, 2621
AjnI	CCWGG	3 cut(s)	1538, 2036, 2217
AjuI	GAANNNNNNNTTGG	4 cut(s)	575, 607, 1094, 1126
Alw21I	GWGCWC	4 cut(s)	544, 1406, 2173, 2346
Alw26I	GTCTC	6 cut(s)	217, 237, 1202, 1489, 1515, 1561
Alw44I	GTGCAC	2 cut(s)	540, 2169
AlwI	GGATC	7 cut(s)	64, 77, 637, 650, 1797, 1922, 2192
Ama87I	CYCGRG	2 cut(s)	7, 1253
Aor13HI	TCCGGA	1 cut(s)	2608
AoxI	GGCC	9 cut(s)	57, 497, 567, 579, 588, 865, 1246, 2284, 2563
ApaLI	GTGCAC	2 cut(s)	540, 2169
ApeKI	GCWGC	7 cut(s)	426, 517, 1105, 1307, 1442, 2107, 2230
ApoI	RAATTY	4 cut(s)	31, 1363, 1490, 1570
ArsI	GACNNNNNNTTYG	2 cut(s)	386, 418
Asp718I	GGTACC	1 cut(s)	1257
AspLEI	GCGC	5 cut(s)	236, 329, 1171, 1523, 2584
AspS9I	GGNCC	8 cut(s)	37, 57, 295, 763, 865, 1283, 1763, 2597
AsuC2I	CCSGG	8 cut(s)	56, 93, 623, 698, 767, 1166, 1254, 1255
AsuHPI	GGTGA	5 cut(s)	1445, 2026, 2231, 2367, 2380
AsuII	TTCGAA	1 cut(s)	273
AvaI	CYCGRG	2 cut(s)	7, 1253
AvaII	GGWCC	6 cut(s)	37, 295, 763, 1283, 1763, 2597
AxyI	CCTNAGG	1 cut(s)	1863
BaeGI	GKGCMC	2 cut(s)	544, 2173
BaeI	ACNNNNGTAYC	4 cut(s)	883, 916, 1241, 1274
BamHI	GGATCC	2 cut(s)	69, 642
BanI	GGYRCC	2 cut(s)	1257, 1369
BanII	GRGCYC	4 cut(s)	787, 1406, 1663, 2346
BauI	CACGAG	1 cut(s)	416
BbsI	GAAGAC	3 cut(s)	255, 1598, 2069
Bbv12I	GWGCWC	4 cut(s)	544, 1406, 2173, 2346
BbvI	GCAGC	7 cut(s)	413, 504, 1117, 1294, 1454, 2094, 2217
BccI	CCATC	4 cut(s)	663, 1274, 1349, 2135
BceAI	ACGGC	5 cut(s)	44, 328, 418, 490, 1864
BciT130I	CCWGG	3 cut(s)	1540, 2038, 2219
BciVI	GTATCC	1 cut(s)	2451
BclI	TGATCA	3 cut(s)	1476, 1672, 2236
BcnI	CCSGG	8 cut(s)	56, 93, 623, 698, 767, 1166, 1254, 1255
BcoDI	GTCTC	6 cut(s)	217, 237, 1202, 1489, 1515, 1561
BfaI	CTAG	2 cut(s)	2006, 2243
BfmI	CTRYAG	1 cut(s)	850
BfuI	GTATCC	1 cut(s)	2451
BglI	GCCNNNNNGGC	1 cut(s)	587
BglII	AGATCT	2 cut(s)	11, 1678
Bme18I	GGWCC	6 cut(s)	37, 295, 763, 1283, 1763, 2597
BmeT110I	CYCGRG	2 cut(s)	7, 1253
BmgT120I	GGNCC	8 cut(s)	37, 57, 295, 763, 865, 1283, 1763, 2597
BmiI	GGNNCC	9 cut(s)	39, 71, 644, 812, 1259, 1371, 1764, 1765, 2121
BmsI	GCATC	6 cut(s)	1129, 1540, 1552, 1706, 2182, 2191
BoxI	GACNNNNGTC	1 cut(s)	452
BpiI	GAAGAC	3 cut(s)	255, 1598, 2069
BplI	GAGNNNNNCTC	2 cut(s)	2333, 2365
Bpu14I	TTCGAA	1 cut(s)	273
BpuEI	CTTGAG	2 cut(s)	700, 1703
BpuMI	CCSGG	8 cut(s)	56, 93, 623, 698, 767, 1166, 1254, 1255
Bsa29I	ATCGAT	1 cut(s)	1278
BsaAI	YACGTR	3 cut(s)	349, 574, 1380
BsaBI	GATNNNNATC	2 cut(s)	1677, 2196
BsaHI	GRCGYC	2 cut(s)	333, 396
BsaI	GGTCTC	1 cut(s)	1202
BsaJI	CCNNGG	8 cut(s)	91, 472, 535, 1177, 1253, 1538, 2057, 2217
BsaWI	WCCGGW	3 cut(s)	750, 760, 2608
BsaXI	ACNNNNNCTCC	2 cut(s)	104, 134
Bsc4I	CCNNNNNNNGG	8 cut(s)	66, 264, 628, 661, 1183, 1773, 2207, 2594
Bse118I	RCCGGY	2 cut(s)	1824, 2266
Bse1I	ACTGG	3 cut(s)	1212, 2178, 2316
Bse21I	CCTNAGG	1 cut(s)	1863
Bse3DI	GCAATG	3 cut(s)	1104, 1794, 2469
Bse8I	GATNNNNATC	2 cut(s)	1677, 2196
BseAI	TCCGGA	1 cut(s)	2608
BseBI	CCWGG	3 cut(s)	1540, 2038, 2219
BseCI	ATCGAT	1 cut(s)	1278
BseDI	CCNNGG	8 cut(s)	91, 472, 535, 1177, 1253, 1538, 2057, 2217
BseJI	GATNNNNATC	2 cut(s)	1677, 2196
BseLI	CCNNNNNNNGG	8 cut(s)	66, 264, 628, 661, 1183, 1773, 2207, 2594
BseMI	GCAATG	3 cut(s)	1104, 1794, 2469
BseMII	CTCAG	4 cut(s)	770, 1877, 1990, 2424
BseNI	ACTGG	3 cut(s)	1212, 2178, 2316
BseRI	GAGGAG	5 cut(s)	401, 785, 1726, 2171, 2354
BseSI	GKGCMC	2 cut(s)	544, 2173
BseXI	GCAGC	7 cut(s)	413, 504, 1117, 1294, 1454, 2094, 2217
BseYI	CCCAGC	2 cut(s)	862, 1719
BsgI	GTGCAG	2 cut(s)	1172, 1211
Bsh1236I	CGCG	4 cut(s)	234, 236, 329, 1171
Bsh1285I	CGRYCG	2 cut(s)	130, 553
BshFI	GGCC	9 cut(s)	59, 499, 569, 581, 590, 867, 1248, 2286, 2565
BshNI	GGYRCC	2 cut(s)	1257, 1369
BshVI	ATCGAT	1 cut(s)	1278
BsiEI	CGRYCG	2 cut(s)	130, 553
BsiHKAI	GWGCWC	4 cut(s)	544, 1406, 2173, 2346
BsiHKCI	CYCGRG	2 cut(s)	7, 1253
BslFI	GGGAC	1 cut(s)	1776
BslI	CCNNNNNNNGG	8 cut(s)	66, 264, 628, 661, 1183, 1773, 2207, 2594
BsmAI	GTCTC	6 cut(s)	217, 237, 1202, 1489, 1515, 1561
BsmBI	CGTCTC	3 cut(s)	217, 237, 1515
BsmFI	GGGAC	1 cut(s)	1776
BsmI	GAATGC	1 cut(s)	864
BsnI	GGCC	9 cut(s)	59, 499, 569, 581, 590, 867, 1248, 2286, 2565
Bso31I	GGTCTC	1 cut(s)	1202
BsoBI	CYCGRG	2 cut(s)	7, 1253
Bsp119I	TTCGAA	1 cut(s)	273
Bsp1286I	GDGCHC	6 cut(s)	544, 787, 1406, 1663, 2173, 2346
Bsp13I	TCCGGA	1 cut(s)	2608
BspACI	CCGC	6 cut(s)	138, 315, 318, 329, 424, 2600
BspANI	GGCC	9 cut(s)	59, 499, 569, 581, 590, 867, 1248, 2286, 2565
BspCNI	CTCAG	4 cut(s)	771, 1876, 1989, 2423
BspDI	ATCGAT	1 cut(s)	1278
BspEI	TCCGGA	1 cut(s)	2608
BspFNI	CGCG	4 cut(s)	234, 236, 329, 1171
BspHI	TCATGA	1 cut(s)	1642
BspLI	GGNNCC	9 cut(s)	39, 71, 644, 812, 1259, 1371, 1764, 1765, 2121
BspPI	GGATC	7 cut(s)	64, 77, 637, 650, 1797, 1922, 2192
BspQI	GCTCTTC	1 cut(s)	1110
BspT104I	TTCGAA	1 cut(s)	273
BspT107I	GGYRCC	2 cut(s)	1257, 1369
BspTNI	GGTCTC	1 cut(s)	1202
BsrDI	GCAATG	3 cut(s)	1104, 1794, 2469
BsrFI	RCCGGY	2 cut(s)	1824, 2266
BsrI	ACTGG	3 cut(s)	1212, 2178, 2316
BssAI	RCCGGY	2 cut(s)	1824, 2266
BssECI	CCNNGG	8 cut(s)	91, 472, 535, 1177, 1253, 1538, 2057, 2217
BssNI	GRCGYC	2 cut(s)	333, 396
BssSI	CACGAG	1 cut(s)	416
BssT1I	CCWWGG	1 cut(s)	2057
Bst2BI	CACGAG	1 cut(s)	416
Bst2UI	CCWGG	3 cut(s)	1540, 2038, 2219
Bst4CI	ACNGT	6 cut(s)	131, 539, 554, 1387, 2168, 2539
Bst6I	CTCTTC	2 cut(s)	1110, 1590
BstACI	GRCGYC	2 cut(s)	333, 396
BstAPI	GCANNNNNTGC	1 cut(s)	1316
BstBAI	YACGTR	3 cut(s)	349, 574, 1380
BstBI	TTCGAA	1 cut(s)	273
BstC8I	GCNNGC	9 cut(s)	87, 431, 532, 544, 567, 1693, 1826, 2466, 2488
BstDEI	CTNAG	5 cut(s)	779, 1514, 1863, 1976, 2410
BstDSI	CCRYGG	2 cut(s)	472, 535
BstFNI	CGCG	4 cut(s)	234, 236, 329, 1171
BstHHI	GCGC	5 cut(s)	236, 329, 1171, 1523, 2584
BstMAI	GTCTC	6 cut(s)	217, 237, 1202, 1489, 1515, 1561
BstMCI	CGRYCG	2 cut(s)	130, 553
BstMWI	GCNNNNNNNGC	4 cut(s)	324, 587, 1316, 1658
BstNI	CCWGG	3 cut(s)	1540, 2038, 2219
BstNSI	RCATGY	1 cut(s)	517
BstPAI	GACNNNNGTC	1 cut(s)	452
BstSFI	CTRYAG	1 cut(s)	850
BstSLI	GKGCMC	2 cut(s)	544, 2173
BstUI	CGCG	4 cut(s)	234, 236, 329, 1171
BstV1I	GCAGC	7 cut(s)	413, 504, 1117, 1294, 1454, 2094, 2217
BstV2I	GAAGAC	3 cut(s)	255, 1598, 2069
BstX2I	RGATCY	4 cut(s)	11, 69, 642, 1678
BstXI	CCANNNNNNTGG	1 cut(s)	1573
BstYI	RGATCY	4 cut(s)	11, 69, 642, 1678
Bsu15I	ATCGAT	1 cut(s)	1278
Bsu36I	CCTNAGG	1 cut(s)	1863
BsuI	GTATCC	1 cut(s)	2451
BsuRI	GGCC	9 cut(s)	59, 499, 569, 581, 590, 867, 1248, 2286, 2565
BsuTUI	ATCGAT	1 cut(s)	1278
BtgI	CCRYGG	2 cut(s)	472, 535
BtsI	GCAGTG	4 cut(s)	1897, 1930, 2000, 2497
Cac8I	GCNNGC	9 cut(s)	87, 431, 532, 544, 567, 1693, 1826, 2466, 2488
CciI	TCATGA	1 cut(s)	1642
CfoI	GCGC	5 cut(s)	236, 329, 1171, 1523, 2584
Cfr10I	RCCGGY	2 cut(s)	1824, 2266
Cfr13I	GGNCC	8 cut(s)	37, 57, 295, 763, 865, 1283, 1763, 2597
Cfr9I	CCCGGG	1 cut(s)	1253
ClaI	ATCGAT	1 cut(s)	1278
CpoI	CGGWCCG	1 cut(s)	763
CseI	GACGC	2 cut(s)	1303, 1592
CsiI	ACCWGGT	1 cut(s)	2036
Csp6I	GTAC	6 cut(s)	346, 555, 691, 892, 1258, 1381
CspI	CGGWCCG	1 cut(s)	763
CviQI	GTAC	6 cut(s)	346, 555, 691, 892, 1258, 1381
DdeI	CTNAG	5 cut(s)	779, 1514, 1863, 1976, 2410
DraIII	CACNNNGTG	2 cut(s)	2040, 2296
DrdI	GACNNNNNNGTC	2 cut(s)	293, 338
DseDI	GACNNNNNNGTC	2 cut(s)	293, 338
EaeI	YGGCCR	2 cut(s)	579, 1246
Eam1104I	CTCTTC	2 cut(s)	1110, 1590
EarI	CTCTTC	2 cut(s)	1110, 1590
Ecl136II	GAGCTC	2 cut(s)	1404, 2344
Eco130I	CCWWGG	1 cut(s)	2057
Eco147I	AGGCCT	3 cut(s)	569, 590, 2565
Eco24I	GRGCYC	4 cut(s)	787, 1406, 1663, 2346
Eco31I	GGTCTC	1 cut(s)	1202
Eco47I	GGWCC	6 cut(s)	37, 295, 763, 1283, 1763, 2597
Eco53kI	GAGCTC	2 cut(s)	1404, 2344
Eco57I	CTGAAG	4 cut(s)	950, 1756, 1854, 2556
Eco81I	CCTNAGG	1 cut(s)	1863
Eco88I	CYCGRG	2 cut(s)	7, 1253
EcoICRI	GAGCTC	2 cut(s)	1404, 2344
EcoO109I	RGGNCCY	1 cut(s)	1763
EcoRII	CCWGG	3 cut(s)	1538, 2036, 2217
EcoT14I	CCWWGG	1 cut(s)	2057
EcoT22I	ATGCAT	1 cut(s)	2396
EcoT38I	GRGCYC	4 cut(s)	787, 1406, 1663, 2346
ErhI	CCWWGG	1 cut(s)	2057
Esp3I	CGTCTC	3 cut(s)	217, 237, 1515
FalI	AAGNNNNNCTT	2 cut(s)	2217, 2249
FaqI	GGGAC	1 cut(s)	1776
FbaI	TGATCA	3 cut(s)	1476, 1672, 2236
FriOI	GRGCYC	4 cut(s)	787, 1406, 1663, 2346
FspBI	CTAG	2 cut(s)	2006, 2243
GlaI	GCGC	5 cut(s)	235, 328, 1170, 1522, 2583
GsaI	CCCAGC	2 cut(s)	866, 1723
HaeIII	GGCC	9 cut(s)	59, 499, 569, 581, 590, 867, 1248, 2286, 2565
HgaI	GACGC	2 cut(s)	1303, 1592
HhaI	GCGC	5 cut(s)	236, 329, 1171, 1523, 2584
Hin1I	GRCGYC	2 cut(s)	333, 396
Hin6I	GCGC	5 cut(s)	234, 327, 1169, 1521, 2582
HinP1I	GCGC	5 cut(s)	234, 327, 1169, 1521, 2582
HincII	GTYRAC	1 cut(s)	460
HindII	GTYRAC	1 cut(s)	460
HindIII	AAGCTT	2 cut(s)	704, 1778
HphI	GGTGA	5 cut(s)	1445, 2026, 2231, 2367, 2380
Hpy166II	GTNNAC	8 cut(s)	460, 542, 557, 1383, 1876, 2043, 2171, 2320
Hpy8I	GTNNAC	8 cut(s)	460, 542, 557, 1383, 1876, 2043, 2171, 2320
Hpy99I	CGWCG	8 cut(s)	128, 338, 398, 401, 451, 739, 742, 1297
HpyAV	CCTTC	6 cut(s)	601, 878, 1000, 1009, 1132, 1960
HpyCH4III	ACNGT	6 cut(s)	131, 539, 554, 1387, 2168, 2539
HpyCH4IV	ACGT	7 cut(s)	174, 333, 348, 396, 573, 636, 1379
HpyF10VI	GCNNNNNNNGC	4 cut(s)	324, 587, 1316, 1658
HpyF3I	CTNAG	5 cut(s)	779, 1514, 1863, 1976, 2410
HpySE526I	ACGT	7 cut(s)	174, 333, 348, 396, 573, 636, 1379
Hsp92I	GRCGYC	2 cut(s)	333, 396
HspAI	GCGC	5 cut(s)	234, 327, 1169, 1521, 2582
KflI	GGGWCCC	1 cut(s)	1763
Kpn2I	TCCGGA	1 cut(s)	2608
KpnI	GGTACC	1 cut(s)	1261
KroI	GCCGGC	1 cut(s)	1824
KroNI	GCCGGC	1 cut(s)	1826
Ksp22I	TGATCA	3 cut(s)	1476, 1672, 2236
LguI	GCTCTTC	1 cut(s)	1110
LmnI	GCTCC	5 cut(s)	1401, 1888, 2075, 2119, 2341
Lsp1109I	GCAGC	7 cut(s)	413, 504, 1117, 1294, 1454, 2094, 2217
LweI	GCATC	6 cut(s)	1129, 1540, 1552, 1706, 2182, 2191
MabI	ACCWGGT	1 cut(s)	2036
MaeI	CTAG	2 cut(s)	2006, 2243
MaeII	ACGT	7 cut(s)	174, 333, 348, 396, 573, 636, 1379
MaeIII	GTNAC	3 cut(s)	1288, 2027, 2373
MboII	GAAGA	9 cut(s)	213, 260, 1045, 1127, 1598, 1607, 2074, 2107, 2110
MflI	RGATCY	4 cut(s)	11, 69, 642, 1678
MhlI	GDGCHC	6 cut(s)	544, 787, 1406, 1663, 2173, 2346
MluCI	AATT	6 cut(s)	31, 793, 1363, 1490, 1570, 1912
MlyI	GAGTC	2 cut(s)	87, 375
MmeI	TCCRAC	9 cut(s)	47, 147, 177, 240, 325, 720, 1404, 1569, 2072
Mph1103I	ATGCAT	1 cut(s)	2396
MroI	TCCGGA	1 cut(s)	2608
MroNI	GCCGGC	1 cut(s)	1824
MseI	TTAA	6 cut(s)	359, 930, 1071, 1341, 2277, 2427
MslI	CAYNNNNRTG	2 cut(s)	1583, 2190
MspA1I	CMGCKG	1 cut(s)	426
Mva1269I	GAATGC	1 cut(s)	864
MvaI	CCWGG	3 cut(s)	1540, 2038, 2219
MvnI	CGCG	4 cut(s)	234, 236, 329, 1171
MwoI	GCNNNNNNNGC	4 cut(s)	324, 587, 1316, 1658
NaeI	GCCGGC	1 cut(s)	1826
NciI	CCSGG	8 cut(s)	56, 93, 623, 698, 767, 1166, 1254, 1255
NgoMIV	GCCGGC	1 cut(s)	1824
NlaIV	GGNNCC	9 cut(s)	39, 71, 644, 812, 1259, 1371, 1764, 1765, 2121
NmeAIII	GCCGAG	1 cut(s)	1202
NmuCI	GTSAC	2 cut(s)	1288, 2373
NsiI	ATGCAT	1 cut(s)	2396
NspI	RCATGY	1 cut(s)	517
NspV	TTCGAA	1 cut(s)	273
PaeR7I	CTCGAG	1 cut(s)	7
PagI	TCATGA	1 cut(s)	1642
PceI	AGGCCT	3 cut(s)	569, 590, 2565
PciSI	GCTCTTC	1 cut(s)	1110
PcsI	WCGNNNNNNNCGW	3 cut(s)	238, 381, 550
PctI	GAATGC	1 cut(s)	864
PdiI	GCCGGC	1 cut(s)	1826
PflFI	GACNNNGTC	1 cut(s)	395
PflMI	CCANNNNNTGG	3 cut(s)	264, 661, 2594
PfoI	TCCNGGA	1 cut(s)	765
PleI	GAGTC	2 cut(s)	86, 374
PpsI	GAGTC	2 cut(s)	86, 374
Ppu21I	YACGTR	3 cut(s)	349, 574, 1380
PpuMI	RGGWCCY	1 cut(s)	1763
PshAI	GACNNNNGTC	1 cut(s)	452
Psp124BI	GAGCTC	2 cut(s)	1406, 2346
Psp1406I	AACGTT	1 cut(s)	174
Psp5II	RGGWCCY	1 cut(s)	1763
Psp6I	CCWGG	3 cut(s)	1538, 2036, 2217
PspFI	CCCAGC	2 cut(s)	862, 1719
PspGI	CCWGG	3 cut(s)	1538, 2036, 2217
PspN4I	GGNNCC	9 cut(s)	39, 71, 644, 812, 1259, 1371, 1764, 1765, 2121
PspPI	GGNCC	8 cut(s)	37, 57, 295, 763, 865, 1283, 1763, 2597
PspPPI	RGGWCCY	1 cut(s)	1763
PsuI	RGATCY	4 cut(s)	11, 69, 642, 1678
PsyI	GACNNNGTC	1 cut(s)	395
RsaI	GTAC	6 cut(s)	347, 556, 692, 893, 1259, 1382
RsaNI	GTAC	6 cut(s)	346, 555, 691, 892, 1258, 1381
RseI	CAYNNNNRTG	2 cut(s)	1583, 2190
Rsr2I	CGGWCCG	1 cut(s)	763
RsrII	CGGWCCG	1 cut(s)	763
SacI	GAGCTC	2 cut(s)	1406, 2346
SapI	GCTCTTC	1 cut(s)	1110
SaqAI	TTAA	6 cut(s)	359, 930, 1071, 1341, 2277, 2427
Sau96I	GGNCC	8 cut(s)	37, 57, 295, 763, 865, 1283, 1763, 2597
SchI	GAGTC	2 cut(s)	87, 375
SduI	GDGCHC	6 cut(s)	544, 787, 1406, 1663, 2173, 2346
SexAI	ACCWGGT	1 cut(s)	2036
SfaNI	GCATC	6 cut(s)	1129, 1540, 1552, 1706, 2182, 2191
SfcI	CTRYAG	1 cut(s)	850
SfiI	GGCCNNNNNGGCC	1 cut(s)	587
Sfr274I	CTCGAG	1 cut(s)	7
SfuI	TTCGAA	1 cut(s)	273
SinI	GGWCC	6 cut(s)	37, 295, 763, 1283, 1763, 2597
SlaI	CTCGAG	1 cut(s)	7
SmaI	CCCGGG	1 cut(s)	1255
SmiMI	CAYNNNNRTG	2 cut(s)	1583, 2190
SmlI	CTYRAG	3 cut(s)	7, 679, 1682
SmoI	CTYRAG	3 cut(s)	7, 679, 1682
Sse9I	AATT	6 cut(s)	31, 793, 1363, 1490, 1570, 1912
SseBI	AGGCCT	3 cut(s)	569, 590, 2565
SsiI	CCGC	6 cut(s)	138, 315, 318, 329, 424, 2600
SspMI	CTAG	2 cut(s)	2006, 2243
SstI	GAGCTC	2 cut(s)	1406, 2346
StuI	AGGCCT	3 cut(s)	569, 590, 2565
StyI	CCWWGG	1 cut(s)	2057
TaaI	ACNGT	6 cut(s)	131, 539, 554, 1387, 2168, 2539
TaiI	ACGT	7 cut(s)	177, 336, 351, 399, 576, 639, 1382
TaqII	GACCGA	1 cut(s)	1894
TasI	AATT	6 cut(s)	31, 793, 1363, 1490, 1570, 1912
TauI	GCSGC	3 cut(s)	141, 318, 321
Tru1I	TTAA	6 cut(s)	359, 930, 1071, 1341, 2277, 2427
Tru9I	TTAA	6 cut(s)	359, 930, 1071, 1341, 2277, 2427
TseFI	GTSAC	2 cut(s)	1288, 2373
TseI	GCWGC	7 cut(s)	426, 517, 1105, 1307, 1442, 2107, 2230
Tsp45I	GTSAC	2 cut(s)	1288, 2373
TspDTI	ATGAA	7 cut(s)	88, 860, 1115, 1376, 1583, 1936, 2617
TspGWI	ACGGA	6 cut(s)	378, 464, 723, 1497, 1517, 2445
TspMI	CCCGGG	1 cut(s)	1253
Tth111I	GACNNNGTC	1 cut(s)	395
Van91I	CCANNNNNTGG	3 cut(s)	264, 661, 2594
VneI	GTGCAC	2 cut(s)	540, 2169
VpaK11BI	GGWCC	6 cut(s)	37, 295, 763, 1283, 1763, 2597
XapI	RAATTY	4 cut(s)	31, 1363, 1490, 1570
XceI	RCATGY	1 cut(s)	517
XhoI	CTCGAG	1 cut(s)	7
XmaI	CCCGGG	1 cut(s)	1253
XspI	CTAG	2 cut(s)	2006, 2243
ZraI	GACGTC	2 cut(s)	334, 397
Zsp2I	ATGCAT	1 cut(s)	2396

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.