Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0017477

Physical Location & Seq

Genomic Coordinates Reverse (-)

26923291 .. 26926307

Gene Structure 3017 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG124100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2628 bp

ATGCTCTCGAGATCTTGCTTTGATTTGCTGAATTTGGACCCCATTGACGATTTCCGGGCCGTCAGTCGGATCCCCAGAGTCATGCAAGCCTCCGGCTTCATGTCCGAGTTCGACAACAACTCCGACGACCGTTCCGGCGGCTCATCCTCATCCGACAACAACAACAACGTTGTTTCCTCTGTTTCGCAGGAACGAAGAATCATCGTCTCCAACCACTTGCCAATACGCGCGTTTCGAGACGAAGACGAAACCAACAAGTGGCGTTTCGAATACGACCACGACAGCTTGGTCCTCCAACTCAAAGACGGCGGCGGCTTTGGCGCGGACGTCGAGGTCTTGTACGTGGGTTGCTTAAACGGAGTCGAGATCGACCCCTGCGATCAAGACGACGTCGCATTTGTCCTCCTCCACGAGTTCCGCTGCGTGCCGTGTTTTCTACCGACGGAAGTCGTCAACAAGTTTTATCACGGCTTCTGTAAGCACTATCTTTGGCCTCTGTTTCACTACATGCTGCCCATGTCAGCCAGCCACGGTGCACGCTTCGACCGTACACTCTGGCAGGCCTACGTGTCGGCCAACAAGGCCTTCGCCGATAGAATCATAGAGGTTCTAAACCCGGATGAGGACTACGTTTGGATCCATGATTACCATCTCATGGTTTTGCCTACTTTCTTGAGGAAAAGGTACTACCGCGTCAAGCTTGGTTTCTTTCTGCATAGTCCTTTTCCGTCGTCGGAGATTTACCGGACGATTCCGGTCCGGGAAGAGATTCTGAGGGCTCTGTTGAATTGTGATCTTGTTGGGTTTCATATATTTGATTATGCGAGGCACTTCTTGTCTTGCTGTAGCAGAATGCTGGATCTTCATTATGAGTTCAAAAGAGGGTACATGGGTTTGGAGTATTATGGTCGAATAGTGAGTATTAAGATGTTGCCTGTGGGGATTCATATGGGTCAGCTTCAGTCTGTTTTGTCGCTTGATGATACTGCCAAGAAAGTGAAGGTGTTGAAGGAAAAGTTTGAGGGGAAGATTGTGATTTTGGGGGTGGATGATTTGGACTTGTTTAAGGGGATTAGTTTGAAGTTTCTGGCAATGAAGCAGCTTTTGGAAGAGCATCAGAGTTTGAGGGGGAAGGTGGTTATGGTGCAGATAACAAACCCGGCACGAAGCCGAGGCAAGGATGTGCAGGAGGTTCTGAATGAGACCAGTGCTATTGCAAAAGAGATCAATGACAAGTATGGCCAACCCGGGTATCAGCCCCTTGTTGTTATCGATGGACCTGTGACGACGCAAGACAAAGCTGCATATTATGCCATTTCGGAGTGCTGTTTGGTTAATGCTGTGAGGGATGGGATGAATTTGGTGCCTTACACGTACACTGTTTGTAGGCAGGGGAGCTCTGTTTTGGACAAGGTGTTGGAGATTGGTGATGAGGCAGCAGAAACGACACCTAAACCGAAAAAGAGTGTGATCATTGTCTCTGAATTTATTGGCTGTTCTCCGTCTCTTAGTGGCGCAATCCGTGTCAATCCCTGGAACATTGATGCTGTCTCTGATGCCATGAATTTGGTCATCACAATGTCGGAGGAAGAGAAGCGTCTTCGCCATGACAAACATTACAAGTATATCAGCTCTCATGATGTTGCTTATTGGGCTCGGAGTTTTGATCAAGATCTTGAGAGAGCTTGCAGAGAGCATTATCGGAGGAGATGCTGGGGCATCGGTTTAGGTTTGGGATTCAGAGTTGTAGCTTTGGGACCCAACTTCAGGAAGCTTTCTGTGGATCACATTGCCTATGCTTACAGAAATACAAGTAGCCGGCTCATCTTATTGGACTATGACGGCACTATGACACCTCAGGCTTCAGTGGACAAAGCTCCAAGCAGTGAAGTTATTTCGGTCTTGAATTGCTTATGCAGTGATCCGAAAAACATTGTTTTCATTGTGAGTGGCAGAGGGAAGGATTCTCTCAGCAAGTGGTTTTCGCAGTGTGAGAAACTAGGTTTATCAGCAGAGCATGGTTACTTCACCAGGTGGACTAAAGATTCTCCTTGGAAGACTTGCTCCTTGACAATGGATTTCAGTTGGAAGAAGATTGTGCTGCCTATTATGGAGCCTTACACAGAAGCAACCGATGGTTCTTTTATAGAAGAGAAAGACAGTGCACTGGTCTGGCATCATCAAGATGCTGATCCTCATTTCGGTTCATCCCAGGCTAAAGAGCTGCTTGATCACCTAGAGAGTGTGCTTGTCAATGAGCCGGTTGTAGTTAAAAGAGGCCAACACATTGTGGAAGTGAAACCACTGGATGTGAACAAAGGTATAGTTGTGGAGGAGCTCATCTCCACTATGCGAAATAGGGTGAAGTCACCAGATTTCTTGTTATGCATAGGGGATGATCGCTCAGATGAGGATATGTTTAAGAGCATCATGTATCCCGAATCAAGTCCGTCCTTGCCAGCCATTGCAGAAGTCTTTGCTTGCACTGTTGGTCAAAAACCAAGCATGGCAAAATACTATCTGGATGATACAGTTGATGTCATCAAGTTGCTTCAAGGCCTCGCTTCAGCATCGGTACAACCAAAGTCTGGACCGCAGTTTCCGGAAGCATTTGAAGTGTTCATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005975 GO:0005984 GO:0005991 GO:0005992 GO:0006793 GO:0006796 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009311 GO:0009312 GO:0009987 GO:0016051 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0033554 GO:0034637 GO:0042578 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044262 GO:0044424 GO:0044444 GO:0044464 GO:0046351 GO:0050896 GO:0051716 GO:0070413 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glyco_transf_20 Trehalose_PPase

Protein Analysis

875

Amino Acids

99.25

Weight (kDa)

5.84

Isoelectric Point (pI)

50.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_20	PF00982	65 - 560	9.6e-176	Glycosyltransferase family 20
Trehalose_PPase	PF02358	610 - 844	7.9e-81	Trehalose-phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017477)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G18700
fragaria_vesca	FvH4_4g09780
prunus_persica	Prupe.1G095500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0404641
rosa_laevigata	RLG00000008917
rosa_multiflora	Rmu_sc0002699.1_g000030
rosa_roxburghii	Rroxscaffold_5G00348790
rosa_rugosa	Rorug04G0048300
rosa_samantha	Rh4AG124100 Rh4BG118100 Rh4CG132200
rosa_wichuraiana	Rw4G010060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	287, 332
AatII	GACGTC	2 cut(s)	330, 393
AccB1I	GGYRCC	1 cut(s)	1363
AccB7I	CCANNNNNTGG	3 cut(s)	258, 655, 2588
AccII	CGCG	4 cut(s)	228, 230, 323, 693
AccIII	TCCGGA	1 cut(s)	2602
AciI	CCGC	7 cut(s)	138, 309, 312, 323, 418, 691, 2594
AclI	AACGTT	1 cut(s)	168
AclWI	GGATC	8 cut(s)	64, 77, 631, 644, 867, 1791, 1916, 2186
AcoI	YGGCCR	2 cut(s)	573, 1240
AcsI	RAATTY	4 cut(s)	31, 1357, 1484, 1564
AcuI	CTGAAG	4 cut(s)	944, 1750, 1848, 2550
AcyI	GRCGYC	2 cut(s)	327, 390
AdeI	CACNNNGTG	2 cut(s)	2034, 2290
AfaI	GTAC	6 cut(s)	341, 550, 686, 887, 1376, 2577
AfiI	CCNNNNNNNGG	8 cut(s)	66, 258, 622, 655, 1177, 1767, 2201, 2588
AflIII	ACRYGT	2 cut(s)	567, 1371
AgsI	TTSAA	7 cut(s)	787, 877, 1009, 1081, 1906, 2555, 2615
AjnI	CCWGG	3 cut(s)	1532, 2030, 2211
AjuI	GAANNNNNNNTTGG	4 cut(s)	569, 601, 1088, 1120
Alw21I	GWGCWC	4 cut(s)	538, 1400, 2167, 2340
Alw26I	GTCTC	6 cut(s)	211, 231, 1196, 1483, 1509, 1555
Alw44I	GTGCAC	2 cut(s)	534, 2163
AlwI	GGATC	8 cut(s)	64, 77, 631, 644, 867, 1791, 1916, 2186
Ama87I	CYCGRG	2 cut(s)	7, 1247
Aor13HI	TCCGGA	1 cut(s)	2602
AoxI	GGCC	8 cut(s)	57, 491, 561, 573, 582, 1240, 2278, 2557
ApaLI	GTGCAC	2 cut(s)	534, 2163
ApeKI	GCWGC	7 cut(s)	420, 511, 1099, 1301, 1436, 2101, 2224
ApoI	RAATTY	4 cut(s)	31, 1357, 1484, 1564
ArsI	GACNNNNNNTTYG	2 cut(s)	380, 412
Asp700I	GAANNNNTTC	1 cut(s)	768
AspLEI	GCGC	3 cut(s)	230, 323, 1517
AspS9I	GGNCC	7 cut(s)	37, 57, 289, 757, 1277, 1757, 2591
AsuC2I	CCSGG	6 cut(s)	56, 617, 761, 1160, 1248, 1249
AsuHPI	GGTGA	5 cut(s)	1439, 2020, 2225, 2361, 2374
AsuII	TTCGAA	1 cut(s)	267
AvaI	CYCGRG	2 cut(s)	7, 1247
AvaII	GGWCC	6 cut(s)	37, 289, 757, 1277, 1757, 2591
AxyI	CCTNAGG	1 cut(s)	1857
BaeGI	GKGCMC	2 cut(s)	538, 2167
BaeI	ACNNNNGTAYC	4 cut(s)	877, 910, 1235, 1268
BalI	TGGCCA	1 cut(s)	1242
BamHI	GGATCC	2 cut(s)	69, 636
BanI	GGYRCC	1 cut(s)	1363
BanII	GRGCYC	4 cut(s)	781, 1400, 1657, 2340
BauI	CACGAG	1 cut(s)	410
BbsI	GAAGAC	3 cut(s)	249, 1592, 2063
Bbv12I	GWGCWC	4 cut(s)	538, 1400, 2167, 2340
BbvI	GCAGC	7 cut(s)	407, 498, 1111, 1288, 1448, 2088, 2211
BccI	CCATC	4 cut(s)	657, 1268, 1343, 2129
BceAI	ACGGC	5 cut(s)	44, 322, 412, 484, 1858
BciT130I	CCWGG	3 cut(s)	1534, 2032, 2213
BciVI	GTATCC	1 cut(s)	2445
BclI	TGATCA	3 cut(s)	1470, 1666, 2230
BcnI	CCSGG	6 cut(s)	56, 617, 761, 1160, 1248, 1249
BcoDI	GTCTC	6 cut(s)	211, 231, 1196, 1483, 1509, 1555
BfaI	CTAG	2 cut(s)	2000, 2237
BfmI	CTRYAG	1 cut(s)	844
BfuI	GTATCC	1 cut(s)	2445
BglI	GCCNNNNNGGC	1 cut(s)	581
BglII	AGATCT	2 cut(s)	11, 1672
Bme1390I	CCNGG	9 cut(s)	56, 617, 761, 1160, 1248, 1249, 1534, 2032, 2213
Bme18I	GGWCC	6 cut(s)	37, 289, 757, 1277, 1757, 2591
BmeT110I	CYCGRG	2 cut(s)	7, 1247
BmgT120I	GGNCC	7 cut(s)	37, 57, 289, 757, 1277, 1757, 2591
BmiI	GGNNCC	7 cut(s)	39, 71, 638, 1365, 1758, 1759, 2115
BmrFI	CCNGG	9 cut(s)	56, 617, 761, 1160, 1248, 1249, 1534, 2032, 2213
BmsI	GCATC	9 cut(s)	1123, 1534, 1546, 1700, 1728, 2176, 2185, 2436, 2579
BoxI	GACNNNNGTC	1 cut(s)	446
BpiI	GAAGAC	3 cut(s)	249, 1592, 2063
BplI	GAGNNNNNCTC	2 cut(s)	2327, 2359
Bpu14I	TTCGAA	1 cut(s)	267
BpuEI	CTTGAG	2 cut(s)	694, 1697
BpuMI	CCSGG	6 cut(s)	56, 617, 761, 1160, 1248, 1249
Bsa29I	ATCGAT	1 cut(s)	1272
BsaAI	YACGTR	3 cut(s)	343, 568, 1374
BsaBI	GATNNNNATC	3 cut(s)	648, 1671, 2190
BsaHI	GRCGYC	2 cut(s)	327, 390
BsaI	GGTCTC	1 cut(s)	1196
BsaJI	CCNNGG	6 cut(s)	529, 1171, 1247, 1532, 2051, 2211
BsaWI	WCCGGW	3 cut(s)	744, 754, 2602
BsaXI	ACNNNNNCTCC	2 cut(s)	104, 134
Bsc4I	CCNNNNNNNGG	8 cut(s)	66, 258, 622, 655, 1177, 1767, 2201, 2588
Bse118I	RCCGGY	2 cut(s)	1818, 2260
Bse1I	ACTGG	3 cut(s)	1206, 2172, 2310
Bse21I	CCTNAGG	1 cut(s)	1857
Bse3DI	GCAATG	3 cut(s)	1098, 1788, 2463
Bse8I	GATNNNNATC	3 cut(s)	648, 1671, 2190
BseAI	TCCGGA	1 cut(s)	2602
BseBI	CCWGG	3 cut(s)	1534, 2032, 2213
BseCI	ATCGAT	1 cut(s)	1272
BseDI	CCNNGG	6 cut(s)	529, 1171, 1247, 1532, 2051, 2211
BseJI	GATNNNNATC	3 cut(s)	648, 1671, 2190
BseLI	CCNNNNNNNGG	8 cut(s)	66, 258, 622, 655, 1177, 1767, 2201, 2588
BseMI	GCAATG	3 cut(s)	1098, 1788, 2463
BseMII	CTCAG	4 cut(s)	764, 1871, 1984, 2418
BseNI	ACTGG	3 cut(s)	1206, 2172, 2310
BseRI	GAGGAG	3 cut(s)	395, 1720, 2348
BseSI	GKGCMC	2 cut(s)	538, 2167
BseXI	GCAGC	7 cut(s)	407, 498, 1111, 1288, 1448, 2088, 2211
BseYI	CCCAGC	1 cut(s)	1713
BsgI	GTGCAG	2 cut(s)	1166, 1205
Bsh1236I	CGCG	4 cut(s)	228, 230, 323, 693
Bsh1285I	CGRYCG	2 cut(s)	130, 547
BshFI	GGCC	8 cut(s)	59, 493, 563, 575, 584, 1242, 2280, 2559
BshNI	GGYRCC	1 cut(s)	1363
BshVI	ATCGAT	1 cut(s)	1272
BsiEI	CGRYCG	2 cut(s)	130, 547
BsiHKAI	GWGCWC	4 cut(s)	538, 1400, 2167, 2340
BsiHKCI	CYCGRG	2 cut(s)	7, 1247
BslFI	GGGAC	1 cut(s)	1770
BslI	CCNNNNNNNGG	8 cut(s)	66, 258, 622, 655, 1177, 1767, 2201, 2588
BsmAI	GTCTC	6 cut(s)	211, 231, 1196, 1483, 1509, 1555
BsmBI	CGTCTC	3 cut(s)	211, 231, 1509
BsmFI	GGGAC	1 cut(s)	1770
BsmI	GAATGC	1 cut(s)	858
BsnI	GGCC	8 cut(s)	59, 493, 563, 575, 584, 1242, 2280, 2559
Bso31I	GGTCTC	1 cut(s)	1196
BsoBI	CYCGRG	2 cut(s)	7, 1247
Bsp119I	TTCGAA	1 cut(s)	267
Bsp1286I	GDGCHC	6 cut(s)	538, 781, 1400, 1657, 2167, 2340
Bsp13I	TCCGGA	1 cut(s)	2602
BspACI	CCGC	7 cut(s)	138, 309, 312, 323, 418, 691, 2594
BspANI	GGCC	8 cut(s)	59, 493, 563, 575, 584, 1242, 2280, 2559
BspCNI	CTCAG	4 cut(s)	765, 1870, 1983, 2417
BspDI	ATCGAT	1 cut(s)	1272
BspEI	TCCGGA	1 cut(s)	2602
BspFNI	CGCG	4 cut(s)	228, 230, 323, 693
BspHI	TCATGA	1 cut(s)	1636
BspLI	GGNNCC	7 cut(s)	39, 71, 638, 1365, 1758, 1759, 2115
BspPI	GGATC	8 cut(s)	64, 77, 631, 644, 867, 1791, 1916, 2186
BspQI	GCTCTTC	1 cut(s)	1104
BspT104I	TTCGAA	1 cut(s)	267
BspT107I	GGYRCC	1 cut(s)	1363
BspTNI	GGTCTC	1 cut(s)	1196
BsrDI	GCAATG	3 cut(s)	1098, 1788, 2463
BsrFI	RCCGGY	2 cut(s)	1818, 2260
BsrI	ACTGG	3 cut(s)	1206, 2172, 2310
BssAI	RCCGGY	2 cut(s)	1818, 2260
BssECI	CCNNGG	6 cut(s)	529, 1171, 1247, 1532, 2051, 2211
BssNI	GRCGYC	2 cut(s)	327, 390
BssSI	CACGAG	1 cut(s)	410
BssT1I	CCWWGG	1 cut(s)	2051
Bst2BI	CACGAG	1 cut(s)	410
Bst2UI	CCWGG	3 cut(s)	1534, 2032, 2213
Bst4CI	ACNGT	7 cut(s)	131, 533, 548, 1381, 2162, 2488, 2533
Bst6I	CTCTTC	4 cut(s)	759, 1104, 1584, 2145
BstACI	GRCGYC	2 cut(s)	327, 390
BstAPI	GCANNNNNTGC	1 cut(s)	1310
BstBAI	YACGTR	3 cut(s)	343, 568, 1374
BstBI	TTCGAA	1 cut(s)	267
BstC8I	GCNNGC	9 cut(s)	87, 425, 526, 538, 561, 1687, 1820, 2460, 2482
BstDEI	CTNAG	5 cut(s)	773, 1508, 1857, 1970, 2404
BstDSI	CCRYGG	1 cut(s)	529
BstFNI	CGCG	4 cut(s)	228, 230, 323, 693
BstHHI	GCGC	3 cut(s)	230, 323, 1517
BstMAI	GTCTC	6 cut(s)	211, 231, 1196, 1483, 1509, 1555
BstMCI	CGRYCG	2 cut(s)	130, 547
BstMWI	GCNNNNNNNGC	4 cut(s)	318, 581, 1310, 1652
BstNI	CCWGG	3 cut(s)	1534, 2032, 2213
BstNSI	RCATGY	1 cut(s)	511
BstPAI	GACNNNNGTC	1 cut(s)	446
BstSCI	CCNGG	9 cut(s)	54, 615, 759, 1158, 1246, 1247, 1532, 2030, 2211
BstSFI	CTRYAG	1 cut(s)	844
BstSLI	GKGCMC	2 cut(s)	538, 2167
BstUI	CGCG	4 cut(s)	228, 230, 323, 693
BstV1I	GCAGC	7 cut(s)	407, 498, 1111, 1288, 1448, 2088, 2211
BstV2I	GAAGAC	3 cut(s)	249, 1592, 2063
BstX2I	RGATCY	5 cut(s)	11, 69, 636, 859, 1672
BstXI	CCANNNNNNTGG	1 cut(s)	1567
BstYI	RGATCY	5 cut(s)	11, 69, 636, 859, 1672
Bsu15I	ATCGAT	1 cut(s)	1272
Bsu36I	CCTNAGG	1 cut(s)	1857
BsuI	GTATCC	1 cut(s)	2445
BsuRI	GGCC	8 cut(s)	59, 493, 563, 575, 584, 1242, 2280, 2559
BsuTUI	ATCGAT	1 cut(s)	1272
BtgI	CCRYGG	1 cut(s)	529
BtsI	GCAGTG	3 cut(s)	1891, 1924, 1994
Cac8I	GCNNGC	9 cut(s)	87, 425, 526, 538, 561, 1687, 1820, 2460, 2482
CciI	TCATGA	1 cut(s)	1636
CfoI	GCGC	3 cut(s)	230, 323, 1517
Cfr10I	RCCGGY	2 cut(s)	1818, 2260
Cfr13I	GGNCC	7 cut(s)	37, 57, 289, 757, 1277, 1757, 2591
Cfr9I	CCCGGG	1 cut(s)	1247
ClaI	ATCGAT	1 cut(s)	1272
CpoI	CGGWCCG	1 cut(s)	757
CseI	GACGC	3 cut(s)	682, 1297, 1586
CsiI	ACCWGGT	1 cut(s)	2030
Csp6I	GTAC	6 cut(s)	340, 549, 685, 886, 1375, 2576
CspI	CGGWCCG	1 cut(s)	757
CviQI	GTAC	6 cut(s)	340, 549, 685, 886, 1375, 2576
DdeI	CTNAG	5 cut(s)	773, 1508, 1857, 1970, 2404
DraIII	CACNNNGTG	2 cut(s)	2034, 2290
DrdI	GACNNNNNNGTC	2 cut(s)	287, 332
DseDI	GACNNNNNNGTC	2 cut(s)	287, 332
EaeI	YGGCCR	2 cut(s)	573, 1240
Eam1104I	CTCTTC	4 cut(s)	759, 1104, 1584, 2145
EarI	CTCTTC	4 cut(s)	759, 1104, 1584, 2145
Ecl136II	GAGCTC	2 cut(s)	1398, 2338
Eco130I	CCWWGG	1 cut(s)	2051
Eco147I	AGGCCT	3 cut(s)	563, 584, 2559
Eco24I	GRGCYC	4 cut(s)	781, 1400, 1657, 2340
Eco31I	GGTCTC	1 cut(s)	1196
Eco47I	GGWCC	6 cut(s)	37, 289, 757, 1277, 1757, 2591
Eco53kI	GAGCTC	2 cut(s)	1398, 2338
Eco57I	CTGAAG	4 cut(s)	944, 1750, 1848, 2550
Eco81I	CCTNAGG	1 cut(s)	1857
Eco88I	CYCGRG	2 cut(s)	7, 1247
EcoICRI	GAGCTC	2 cut(s)	1398, 2338
EcoO109I	RGGNCCY	1 cut(s)	1757
EcoRII	CCWGG	3 cut(s)	1532, 2030, 2211
EcoT14I	CCWWGG	1 cut(s)	2051
EcoT22I	ATGCAT	1 cut(s)	2390
EcoT38I	GRGCYC	4 cut(s)	781, 1400, 1657, 2340
ErhI	CCWWGG	1 cut(s)	2051
Esp3I	CGTCTC	3 cut(s)	211, 231, 1509
FalI	AAGNNNNNCTT	2 cut(s)	2211, 2243
FaqI	GGGAC	1 cut(s)	1770
FauNDI	CATATG	1 cut(s)	948
FbaI	TGATCA	3 cut(s)	1470, 1666, 2230
FriOI	GRGCYC	4 cut(s)	781, 1400, 1657, 2340
FspBI	CTAG	2 cut(s)	2000, 2237
GlaI	GCGC	3 cut(s)	229, 322, 1516
GsaI	CCCAGC	1 cut(s)	1717
HaeIII	GGCC	8 cut(s)	59, 493, 563, 575, 584, 1242, 2280, 2559
HgaI	GACGC	3 cut(s)	682, 1297, 1586
HhaI	GCGC	3 cut(s)	230, 323, 1517
Hin1I	GRCGYC	2 cut(s)	327, 390
Hin6I	GCGC	3 cut(s)	228, 321, 1515
HinP1I	GCGC	3 cut(s)	228, 321, 1515
HincII	GTYRAC	1 cut(s)	454
HindII	GTYRAC	1 cut(s)	454
HindIII	AAGCTT	2 cut(s)	698, 1772
HphI	GGTGA	5 cut(s)	1439, 2020, 2225, 2361, 2374
Hpy166II	GTNNAC	8 cut(s)	454, 536, 551, 1377, 1870, 2037, 2165, 2314
Hpy8I	GTNNAC	8 cut(s)	454, 536, 551, 1377, 1870, 2037, 2165, 2314
Hpy99I	CGWCG	8 cut(s)	128, 332, 392, 395, 445, 733, 736, 1291
HpyAV	CCTTC	5 cut(s)	595, 994, 1003, 1126, 1954
HpyCH4III	ACNGT	7 cut(s)	131, 533, 548, 1381, 2162, 2488, 2533
HpyCH4IV	ACGT	7 cut(s)	168, 327, 342, 390, 567, 630, 1373
HpyF10VI	GCNNNNNNNGC	4 cut(s)	318, 581, 1310, 1652
HpyF3I	CTNAG	5 cut(s)	773, 1508, 1857, 1970, 2404
HpySE526I	ACGT	7 cut(s)	168, 327, 342, 390, 567, 630, 1373
Hsp92I	GRCGYC	2 cut(s)	327, 390
HspAI	GCGC	3 cut(s)	228, 321, 1515
KflI	GGGWCCC	1 cut(s)	1757
Kpn2I	TCCGGA	1 cut(s)	2602
KroI	GCCGGC	1 cut(s)	1818
KroNI	GCCGGC	1 cut(s)	1820
Ksp22I	TGATCA	3 cut(s)	1470, 1666, 2230
LguI	GCTCTTC	1 cut(s)	1104
LmnI	GCTCC	5 cut(s)	1395, 1882, 2069, 2113, 2335
Lsp1109I	GCAGC	7 cut(s)	407, 498, 1111, 1288, 1448, 2088, 2211
LweI	GCATC	9 cut(s)	1123, 1534, 1546, 1700, 1728, 2176, 2185, 2436, 2579
MabI	ACCWGGT	1 cut(s)	2030
MaeI	CTAG	2 cut(s)	2000, 2237
MaeII	ACGT	7 cut(s)	168, 327, 342, 390, 567, 630, 1373
MaeIII	GTNAC	3 cut(s)	1282, 2021, 2367
MflI	RGATCY	5 cut(s)	11, 69, 636, 859, 1672
MhlI	GDGCHC	6 cut(s)	538, 781, 1400, 1657, 2167, 2340
MlsI	TGGCCA	1 cut(s)	1242
MluCI	AATT	6 cut(s)	31, 787, 1357, 1484, 1564, 1906
MluNI	TGGCCA	1 cut(s)	1242
MlyI	GAGTC	2 cut(s)	87, 369
MmeI	TCCRAC	9 cut(s)	47, 147, 177, 234, 319, 714, 1398, 1563, 2066
Mox20I	TGGCCA	1 cut(s)	1242
Mph1103I	ATGCAT	1 cut(s)	2390
MroI	TCCGGA	1 cut(s)	2602
MroNI	GCCGGC	1 cut(s)	1818
MroXI	GAANNNNTTC	1 cut(s)	768
MscI	TGGCCA	1 cut(s)	1242
MseI	TTAA	6 cut(s)	353, 924, 1065, 1335, 2271, 2421
MslI	CAYNNNNRTG	2 cut(s)	1577, 2184
Msp20I	TGGCCA	1 cut(s)	1242
MspA1I	CMGCKG	1 cut(s)	420
MspR9I	CCNGG	9 cut(s)	56, 617, 761, 1160, 1248, 1249, 1534, 2032, 2213
Mva1269I	GAATGC	1 cut(s)	858
MvaI	CCWGG	3 cut(s)	1534, 2032, 2213
MvnI	CGCG	4 cut(s)	228, 230, 323, 693
MwoI	GCNNNNNNNGC	4 cut(s)	318, 581, 1310, 1652
NaeI	GCCGGC	1 cut(s)	1820
NciI	CCSGG	6 cut(s)	56, 617, 761, 1160, 1248, 1249
NdeI	CATATG	1 cut(s)	948
NgoMIV	GCCGGC	1 cut(s)	1818
NlaIV	GGNNCC	7 cut(s)	39, 71, 638, 1365, 1758, 1759, 2115
NmeAIII	GCCGAG	1 cut(s)	1196
NmuCI	GTSAC	2 cut(s)	1282, 2367
NsiI	ATGCAT	1 cut(s)	2390
NspI	RCATGY	1 cut(s)	511
NspV	TTCGAA	1 cut(s)	267
PaeR7I	CTCGAG	1 cut(s)	7
PagI	TCATGA	1 cut(s)	1636
PceI	AGGCCT	3 cut(s)	563, 584, 2559
PciSI	GCTCTTC	1 cut(s)	1104
PcsI	WCGNNNNNNNCGW	3 cut(s)	232, 375, 544
PctI	GAATGC	1 cut(s)	858
PdiI	GCCGGC	1 cut(s)	1820
PdmI	GAANNNNTTC	1 cut(s)	768
PfeI	GAWTC	9 cut(s)	198, 597, 751, 769, 943, 1737, 1964, 2045, 2441
PflFI	GACNNNGTC	1 cut(s)	389
PflMI	CCANNNNNTGG	3 cut(s)	258, 655, 2588
PfoI	TCCNGGA	1 cut(s)	759
PleI	GAGTC	2 cut(s)	86, 368
PpsI	GAGTC	2 cut(s)	86, 368
Ppu21I	YACGTR	3 cut(s)	343, 568, 1374
PpuMI	RGGWCCY	1 cut(s)	1757
PshAI	GACNNNNGTC	1 cut(s)	446
Psp124BI	GAGCTC	2 cut(s)	1400, 2340
Psp1406I	AACGTT	1 cut(s)	168
Psp5II	RGGWCCY	1 cut(s)	1757
Psp6I	CCWGG	3 cut(s)	1532, 2030, 2211
PspFI	CCCAGC	1 cut(s)	1713
PspGI	CCWGG	3 cut(s)	1532, 2030, 2211
PspN4I	GGNNCC	7 cut(s)	39, 71, 638, 1365, 1758, 1759, 2115
PspPI	GGNCC	7 cut(s)	37, 57, 289, 757, 1277, 1757, 2591
PspPPI	RGGWCCY	1 cut(s)	1757
PsuI	RGATCY	5 cut(s)	11, 69, 636, 859, 1672
PsyI	GACNNNGTC	1 cut(s)	389
RsaI	GTAC	6 cut(s)	341, 550, 686, 887, 1376, 2577
RsaNI	GTAC	6 cut(s)	340, 549, 685, 886, 1375, 2576
RseI	CAYNNNNRTG	2 cut(s)	1577, 2184
Rsr2I	CGGWCCG	1 cut(s)	757
RsrII	CGGWCCG	1 cut(s)	757
SacI	GAGCTC	2 cut(s)	1400, 2340
SapI	GCTCTTC	1 cut(s)	1104
SaqAI	TTAA	6 cut(s)	353, 924, 1065, 1335, 2271, 2421
Sau96I	GGNCC	7 cut(s)	37, 57, 289, 757, 1277, 1757, 2591
SchI	GAGTC	2 cut(s)	87, 369
ScrFI	CCNGG	9 cut(s)	56, 617, 761, 1160, 1248, 1249, 1534, 2032, 2213
SduI	GDGCHC	6 cut(s)	538, 781, 1400, 1657, 2167, 2340
SexAI	ACCWGGT	1 cut(s)	2030
SfaNI	GCATC	9 cut(s)	1123, 1534, 1546, 1700, 1728, 2176, 2185, 2436, 2579
SfcI	CTRYAG	1 cut(s)	844
SfiI	GGCCNNNNNGGCC	1 cut(s)	581
Sfr274I	CTCGAG	1 cut(s)	7
SfuI	TTCGAA	1 cut(s)	267
SinI	GGWCC	6 cut(s)	37, 289, 757, 1277, 1757, 2591
SlaI	CTCGAG	1 cut(s)	7
SmaI	CCCGGG	1 cut(s)	1249
SmiMI	CAYNNNNRTG	2 cut(s)	1577, 2184
SmlI	CTYRAG	3 cut(s)	7, 673, 1676
SmoI	CTYRAG	3 cut(s)	7, 673, 1676
Sse9I	AATT	6 cut(s)	31, 787, 1357, 1484, 1564, 1906
SseBI	AGGCCT	3 cut(s)	563, 584, 2559
SsiI	CCGC	7 cut(s)	138, 309, 312, 323, 418, 691, 2594
SspMI	CTAG	2 cut(s)	2000, 2237
SstI	GAGCTC	2 cut(s)	1400, 2340
StuI	AGGCCT	3 cut(s)	563, 584, 2559
StyD4I	CCNGG	9 cut(s)	54, 615, 759, 1158, 1246, 1247, 1532, 2030, 2211
StyI	CCWWGG	1 cut(s)	2051
TaaI	ACNGT	7 cut(s)	131, 533, 548, 1381, 2162, 2488, 2533
TaiI	ACGT	7 cut(s)	171, 330, 345, 393, 570, 633, 1376
TaqII	GACCGA	1 cut(s)	1888
TasI	AATT	6 cut(s)	31, 787, 1357, 1484, 1564, 1906
TauI	GCSGC	3 cut(s)	141, 312, 315
TfiI	GAWTC	9 cut(s)	198, 597, 751, 769, 943, 1737, 1964, 2045, 2441
Tru1I	TTAA	6 cut(s)	353, 924, 1065, 1335, 2271, 2421
Tru9I	TTAA	6 cut(s)	353, 924, 1065, 1335, 2271, 2421
TseFI	GTSAC	2 cut(s)	1282, 2367
TseI	GCWGC	7 cut(s)	420, 511, 1099, 1301, 1436, 2101, 2224
Tsp45I	GTSAC	2 cut(s)	1282, 2367
TspGWI	ACGGA	6 cut(s)	372, 458, 717, 1491, 1511, 2439
TspMI	CCCGGG	1 cut(s)	1247
Tth111I	GACNNNGTC	1 cut(s)	389
Van91I	CCANNNNNTGG	3 cut(s)	258, 655, 2588
VneI	GTGCAC	2 cut(s)	534, 2163
VpaK11BI	GGWCC	6 cut(s)	37, 289, 757, 1277, 1757, 2591
XapI	RAATTY	4 cut(s)	31, 1357, 1484, 1564
XceI	RCATGY	1 cut(s)	511
XhoI	CTCGAG	1 cut(s)	7
XmaI	CCCGGG	1 cut(s)	1247
XmnI	GAANNNNTTC	1 cut(s)	768
XspI	CTAG	2 cut(s)	2000, 2237
ZraI	GACGTC	2 cut(s)	328, 391
Zsp2I	ATGCAT	1 cut(s)	2390

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.