RchiOBHm_Chr4g0404641

Glycosyltransferase family 20

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0017477
Physical Location & Seq
Reverse (-)
24461805 .. 24465601
3797 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37618

Sequence Viewer

Length: 2637 bp
ATGCTCTCGAGATCTTGCTTTGATTTGCTGAATTTGGACCCCATTGACGATTTCCGGGCCGTCAGTCGGATCCCCAGAGTCATGCAAGCCTCCGGCTTCATCTCCGAGTTCGACAACAACTCCGACGACCGTTCCGGCGGCTCATCCTCATCCGACAACAACAACAACAACAACAACAACGTTGTTTCCTCTGTCTCGCAGGAACGAAGAATCATCGTCTCCAACCACTTGCCAATACGCGCGTTTCGAGACGAAGACGAAACCAACAAGTGGCGTTTCGAATACGACCACGACAGCTTGGTCCTCCAACTCAAAGACGGCGGCTTTGGCGCGGACGTCGAGGTCTTGTACGTGGGTTGCTTAAACGGAGTCGAGATCGACCCCTGCGATCAAGACGACGTCGCATTTGTCCTCCTCCACGAGTTCCGCTGCGTGCCGTGTTTTCTACCGACGGAAGTCGTCAACAAGTTTTATCACGGCTTCTGTAAGCACTATCTTTGGCCTCTGTTTCACTACATGCTGCCCATGTCAGCCAGCCACGGTGCACGCTTCGACCGTACACTCTGGCAGGCCTACGTGTCGGCCAACAAGGCCTTCGCCGATAGAATCATAGAGGTTCTAAACCCGGATGAGGACTACGTTTGGATCCATGATTACCATCTGATGGTTTTGCCTACTTTCTTGAGGAAAAGGTACTACCGCGTCAAGCTTGGTTTCTTTCTGCATAGTCCTTTTCCGTCGTCGGAGATTTACCGGACGATTCCGGTCCGGGAAGAGATTCTGAGGGCTCTGTTGAATTGTGATCTTGTTGGGTTTCATATATTTGATTATGCGAGGCACTTCTTGTCTTGCTGTAGCAGAATGCTGGGTCTTCATTACGAGTTCAAAAGAGGGTACATGGGTTTGGAGTATTATGGTCGAATAGTGAGTATTAAGATGTTGCCTGTGGGGATTCATATGGGTCAGCTTCAGTCTGTTTTGTCGCTTGATGATACTGCCAAGAAAGTGAAGGTGCTGAAGGAAAAGTATGAGGGGAAGATTGTGATTTTGGGGGTGGATGATTTGGACTTGTTTAAGGGGATTAGTTTGAAGTTTCTGGCAATGAAGCAGCTTTTGGAAGAGCATCAGAGCTTGAGGGGGAAGGTGGTTATGGTGCAGATAACAAACCCAGCACGAAGCCGGGGCAAGGATGTGCAGGAGGTTCTGAATGAGACCAGTGCTATTGCAAAAGAGATCAATGACAAGTATGGCCAACCCGGGTATCAGCCCCTTGTTGTCATCGATGGACCTGTGACGACGCAAGACAAAGCTGCATATTATGCCATTTCGGAGTGCTGTTTGGTTAATGCTGTGAGGGATGGGATGAATTTGGTGCCTTACACGTACACTGTTTGTAGGCAGGGGAGCTCTGTTTTGGACAAGGTGTTGGAGATTGGTGATGAGGCAGCAGATACGACACCTAAACCGAAAAAGAGTGTGATCATTGTCTCTGAATTTATTGGCTGTTCTCCGTCTCTTAGTGGCGCAATCCGTGTCAATCCCTGGAACATTGATGCTGTCTCTGATGCCATGAATTTGGTCATCACAATGTCGGAGGAAGAGAAGCGTCTTCGCCATGACAAACATTACAAGTATATCAGCTCTCATGATGTTGCTTATTGGGCTCGGAGTTTTGATCAAGATCTTGAGAGAGCTTGCAGAGAGCATTATCGGAGGAGATGCTGGGGCATCGGTTTAGGTTTGGGATTCAGAGTTGTAGCTTTGGGACCCAACTTCAGGAAGCTTTCTGTGGATCACATTGCCTATGCTTACAGAAATACAAGTAGCCGGCTCATCTTATTGGACTATGACGGCACTATGACACCTCAGGCTTCAGTGGACAAAGCTCCAAGCAGTGAAGTTATTTCGGTCTTGAATTGCTTATGCAGTGATACGAAAAACATTGTTTTCATTGTGAGTGGCAGAGGGAAGGATTCTCTCAGCAAGTGGTTTTCGCAGTGTGAGAAACTAGGTTTATCAGCAGAGCATGGTTACTTCACCAGGTGGACTAAAGATTCTCCTTGGAAGACTTGCTCCTTGACAATGGATTTCAGTTGGAAGAAGATTGTGCTGCCTATTATGGAGCCTTACACAGAAGCAACCGATGGTTCTTTTATAGAAGAGAAAGACAGTGCACTGGTCTGGCATCATCAAGATGCTGATCCTCATTTCGGCTCATCCCAGGCTAAAGAGCTGCTTGATCACCTAGAGAGTGTGCTTGTCAATGAGCCGGTTGTAGTTAAAAGAGGCCAACACATTGTGGAAGTGAAACCACTGGATGTGAACAAAGGTATAGTTGTGGAGGAGCTCATCTCCACTATGCGAAATAGGTTGAAGTCACCAGATTTCTTGTTATGCATAGGGGATGATCGCTCAGATGAGGATATGTTTAAGAGCATCATGTATCCCGAATCAAGTCCGTCCTTGCCAGCCATTGCAGAAGTCTTTGCTTGCACTGTTGGTCAAAAACCAAGCATGGCAAAATACTATCTGGATGATACAGTTGATGTCATCAAGTTGCTTCAAGGCCTCGCTTCGGCATCGGCACAACCAAAGTCTGGACCGCAGTTTCCGGAAGCATTTGAAGTGTTCATTTGA

Protein Analysis

878

Amino Acids

99.56

Weight (kDa)

5.89

Isoelectric Point (pI)

48.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_20 PF00982 69 - 563 2.2e-176 Glycosyltransferase family 20
Trehalose_PPase PF02358 613 - 847 5.4e-80 Trehalose-phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18700
fragaria_vesca FvH4_4g09780
prunus_persica Prupe.1G095500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0404641
rosa_laevigata RLG00000008917
rosa_multiflora Rmu_sc0002699.1_g000030
rosa_roxburghii Rroxscaffold_5G00348790
rosa_rugosa Rorug04G0048300
rosa_samantha Rh4AG124100 Rh4BG118100 Rh4CG132200
rosa_wichuraiana Rw4G010060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 299, 341
AatII GACGTC 2 cut(s) 339, 402
AccB1I GGYRCC 1 cut(s) 1372
AccB7I CCANNNNNTGG 3 cut(s) 270, 664, 2597
AccII CGCG 4 cut(s) 240, 242, 332, 702
AccIII TCCGGA 1 cut(s) 2611
AciI CCGC 6 cut(s) 138, 321, 332, 427, 700, 2603
AclI AACGTT 1 cut(s) 180
AclWI GGATC 6 cut(s) 64, 77, 640, 653, 1800, 2195
AcoI YGGCCR 2 cut(s) 582, 1249
AcsI RAATTY 4 cut(s) 31, 1366, 1493, 1573
AcuI CTGAAG 4 cut(s) 953, 1037, 1759, 1857
AcyI GRCGYC 2 cut(s) 336, 399
AdeI CACNNNGTG 2 cut(s) 2043, 2299
AfaI GTAC 5 cut(s) 350, 559, 695, 896, 1385
AfiI CCNNNNNNNGG 9 cut(s) 66, 270, 631, 664, 1186, 1776, 2210, 2575, 2597
AflIII ACRYGT 2 cut(s) 576, 1380
AgsI TTSAA 7 cut(s) 796, 886, 1090, 1915, 2374, 2564, 2624
AjnI CCWGG 3 cut(s) 1541, 2039, 2220
AjuI GAANNNNNNNTTGG 4 cut(s) 578, 610, 1097, 1129
Alw21I GWGCWC 4 cut(s) 547, 1409, 2176, 2349
Alw26I GTCTC 7 cut(s) 199, 223, 243, 1205, 1492, 1518, 1564
Alw44I GTGCAC 2 cut(s) 543, 2172
AlwI GGATC 6 cut(s) 64, 77, 640, 653, 1800, 2195
Ama87I CYCGRG 2 cut(s) 7, 1256
Aor13HI TCCGGA 1 cut(s) 2611
AoxI GGCC 8 cut(s) 57, 500, 570, 582, 591, 1249, 2287, 2566
ApaLI GTGCAC 2 cut(s) 543, 2172
ApeKI GCWGC 7 cut(s) 429, 520, 1108, 1310, 1445, 2110, 2233
ApoI RAATTY 4 cut(s) 31, 1366, 1493, 1573
ArsI GACNNNNNNTTYG 4 cut(s) 308, 340, 389, 421
Asp700I GAANNNNTTC 1 cut(s) 777
AspLEI GCGC 3 cut(s) 242, 332, 1526
AspS9I GGNCC 7 cut(s) 37, 57, 301, 766, 1286, 1766, 2600
AsuC2I CCSGG 6 cut(s) 56, 626, 770, 1181, 1257, 1258
AsuHPI GGTGA 4 cut(s) 1448, 2029, 2234, 2370
AsuII TTCGAA 1 cut(s) 279
AvaI CYCGRG 2 cut(s) 7, 1256
AvaII GGWCC 6 cut(s) 37, 301, 766, 1286, 1766, 2600
AxyI CCTNAGG 1 cut(s) 1866
BaeGI GKGCMC 2 cut(s) 547, 2176
BaeI ACNNNNGTAYC 4 cut(s) 886, 919, 1244, 1277
BalI TGGCCA 1 cut(s) 1251
BamHI GGATCC 2 cut(s) 69, 645
BanI GGYRCC 1 cut(s) 1372
BanII GRGCYC 4 cut(s) 790, 1409, 1666, 2349
BauI CACGAG 1 cut(s) 419
BbsI GAAGAC 4 cut(s) 261, 863, 1601, 2072
Bbv12I GWGCWC 4 cut(s) 547, 1409, 2176, 2349
BbvI GCAGC 7 cut(s) 416, 507, 1120, 1297, 1457, 2097, 2220
BccI CCATC 5 cut(s) 658, 666, 1277, 1352, 2138
BceAI ACGGC 5 cut(s) 44, 334, 421, 493, 1867
BciT130I CCWGG 3 cut(s) 1543, 2041, 2222
BciVI GTATCC 1 cut(s) 2454
BclI TGATCA 3 cut(s) 1479, 1675, 2239
BcnI CCSGG 6 cut(s) 56, 626, 770, 1181, 1257, 1258
BcoDI GTCTC 7 cut(s) 199, 223, 243, 1205, 1492, 1518, 1564
BfaI CTAG 2 cut(s) 2009, 2246
BfmI CTRYAG 1 cut(s) 853
BfuI GTATCC 1 cut(s) 2454
BglI GCCNNNNNGGC 1 cut(s) 590
BglII AGATCT 2 cut(s) 11, 1681
BisI GCNGC 9 cut(s) 139, 322, 430, 521, 1109, 1311, 1446, 2111, 2234
BlsI GCNGC 9 cut(s) 140, 323, 431, 522, 1110, 1312, 1447, 2112, 2235
Bme1390I CCNGG 9 cut(s) 56, 626, 770, 1181, 1257, 1258, 1543, 2041, 2222
Bme18I GGWCC 6 cut(s) 37, 301, 766, 1286, 1766, 2600
BmeT110I CYCGRG 2 cut(s) 7, 1256
BmgT120I GGNCC 7 cut(s) 37, 57, 301, 766, 1286, 1766, 2600
BmiI GGNNCC 7 cut(s) 39, 71, 647, 1374, 1767, 1768, 2124
BmrFI CCNGG 9 cut(s) 56, 626, 770, 1181, 1257, 1258, 1543, 2041, 2222
BmsI GCATC 9 cut(s) 1132, 1543, 1555, 1709, 1737, 2185, 2194, 2445, 2588
BoxI GACNNNNGTC 1 cut(s) 455
BpiI GAAGAC 4 cut(s) 261, 863, 1601, 2072
BplI GAGNNNNNCTC 2 cut(s) 2336, 2368
Bpu14I TTCGAA 1 cut(s) 279
BpuEI CTTGAG 3 cut(s) 703, 1153, 1706
BpuMI CCSGG 6 cut(s) 56, 626, 770, 1181, 1257, 1258
Bsa29I ATCGAT 1 cut(s) 1281
BsaAI YACGTR 3 cut(s) 352, 577, 1383
BsaBI GATNNNNATC 3 cut(s) 657, 1680, 2199
BsaHI GRCGYC 2 cut(s) 336, 399
BsaI GGTCTC 1 cut(s) 1205
BsaJI CCNNGG 6 cut(s) 538, 1180, 1256, 1541, 2060, 2220
BsaWI WCCGGW 3 cut(s) 753, 763, 2611
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 9 cut(s) 66, 270, 631, 664, 1186, 1776, 2210, 2575, 2597
Bse118I RCCGGY 2 cut(s) 1827, 2269
Bse1I ACTGG 3 cut(s) 1215, 2181, 2319
Bse21I CCTNAGG 1 cut(s) 1866
Bse3DI GCAATG 3 cut(s) 1107, 1797, 2472
Bse8I GATNNNNATC 3 cut(s) 657, 1680, 2199
BseAI TCCGGA 1 cut(s) 2611
BseBI CCWGG 3 cut(s) 1543, 2041, 2222
BseCI ATCGAT 1 cut(s) 1281
BseDI CCNNGG 6 cut(s) 538, 1180, 1256, 1541, 2060, 2220
BseJI GATNNNNATC 3 cut(s) 657, 1680, 2199
BseLI CCNNNNNNNGG 9 cut(s) 66, 270, 631, 664, 1186, 1776, 2210, 2575, 2597
BseMI GCAATG 3 cut(s) 1107, 1797, 2472
BseMII CTCAG 4 cut(s) 773, 1880, 1993, 2427
BseNI ACTGG 3 cut(s) 1215, 2181, 2319
BseRI GAGGAG 3 cut(s) 404, 1729, 2357
BseSI GKGCMC 2 cut(s) 547, 2176
BseXI GCAGC 7 cut(s) 416, 507, 1120, 1297, 1457, 2097, 2220
BseYI CCCAGC 3 cut(s) 865, 1168, 1722
BsgI GTGCAG 2 cut(s) 1175, 1214
Bsh1236I CGCG 4 cut(s) 240, 242, 332, 702
Bsh1285I CGRYCG 2 cut(s) 130, 556
BshFI GGCC 8 cut(s) 59, 502, 572, 584, 593, 1251, 2289, 2568
BshNI GGYRCC 1 cut(s) 1372
BshVI ATCGAT 1 cut(s) 1281
BsiEI CGRYCG 2 cut(s) 130, 556
BsiHKAI GWGCWC 4 cut(s) 547, 1409, 2176, 2349
BsiHKCI CYCGRG 2 cut(s) 7, 1256
BslFI GGGAC 1 cut(s) 1779
BslI CCNNNNNNNGG 9 cut(s) 66, 270, 631, 664, 1186, 1776, 2210, 2575, 2597
BsmAI GTCTC 7 cut(s) 199, 223, 243, 1205, 1492, 1518, 1564
BsmBI CGTCTC 3 cut(s) 223, 243, 1518
BsmFI GGGAC 1 cut(s) 1779
BsmI GAATGC 1 cut(s) 867
BsnI GGCC 8 cut(s) 59, 502, 572, 584, 593, 1251, 2289, 2568
Bso31I GGTCTC 1 cut(s) 1205
BsoBI CYCGRG 2 cut(s) 7, 1256
Bsp119I TTCGAA 1 cut(s) 279
Bsp1286I GDGCHC 6 cut(s) 547, 790, 1409, 1666, 2176, 2349
Bsp13I TCCGGA 1 cut(s) 2611
BspACI CCGC 6 cut(s) 138, 321, 332, 427, 700, 2603
BspANI GGCC 8 cut(s) 59, 502, 572, 584, 593, 1251, 2289, 2568
BspCNI CTCAG 4 cut(s) 774, 1879, 1992, 2426
BspDI ATCGAT 1 cut(s) 1281
BspEI TCCGGA 1 cut(s) 2611
BspFNI CGCG 4 cut(s) 240, 242, 332, 702
BspHI TCATGA 1 cut(s) 1645
BspLI GGNNCC 7 cut(s) 39, 71, 647, 1374, 1767, 1768, 2124
BspPI GGATC 6 cut(s) 64, 77, 640, 653, 1800, 2195
BspQI GCTCTTC 1 cut(s) 1113
BspT104I TTCGAA 1 cut(s) 279
BspT107I GGYRCC 1 cut(s) 1372
BspTNI GGTCTC 1 cut(s) 1205
BsrDI GCAATG 3 cut(s) 1107, 1797, 2472
BsrFI RCCGGY 2 cut(s) 1827, 2269
BsrI ACTGG 3 cut(s) 1215, 2181, 2319
BssAI RCCGGY 2 cut(s) 1827, 2269
BssECI CCNNGG 6 cut(s) 538, 1180, 1256, 1541, 2060, 2220
BssNI GRCGYC 2 cut(s) 336, 399
BssSI CACGAG 1 cut(s) 419
BssT1I CCWWGG 1 cut(s) 2060
Bst2BI CACGAG 1 cut(s) 419
Bst2UI CCWGG 3 cut(s) 1543, 2041, 2222
Bst4CI ACNGT 7 cut(s) 131, 542, 557, 1390, 2171, 2497, 2542
Bst6I CTCTTC 4 cut(s) 768, 1113, 1593, 2154
BstACI GRCGYC 2 cut(s) 336, 399
BstAPI GCANNNNNTGC 1 cut(s) 1319
BstBAI YACGTR 3 cut(s) 352, 577, 1383
BstBI TTCGAA 1 cut(s) 279
BstC8I GCNNGC 9 cut(s) 87, 434, 535, 547, 570, 1696, 1829, 2469, 2491
BstDEI CTNAG 5 cut(s) 782, 1517, 1866, 1979, 2413
BstDSI CCRYGG 1 cut(s) 538
BstFNI CGCG 4 cut(s) 240, 242, 332, 702
BstHHI GCGC 3 cut(s) 242, 332, 1526
BstMAI GTCTC 7 cut(s) 199, 223, 243, 1205, 1492, 1518, 1564
BstMCI CGRYCG 2 cut(s) 130, 556
BstMWI GCNNNNNNNGC 4 cut(s) 327, 590, 1319, 1661
BstNI CCWGG 3 cut(s) 1543, 2041, 2222
BstNSI RCATGY 1 cut(s) 520
BstPAI GACNNNNGTC 1 cut(s) 455
BstSCI CCNGG 9 cut(s) 54, 624, 768, 1179, 1255, 1256, 1541, 2039, 2220
BstSFI CTRYAG 1 cut(s) 853
BstSLI GKGCMC 2 cut(s) 547, 2176
BstUI CGCG 4 cut(s) 240, 242, 332, 702
BstV1I GCAGC 7 cut(s) 416, 507, 1120, 1297, 1457, 2097, 2220
BstV2I GAAGAC 4 cut(s) 261, 863, 1601, 2072
BstX2I RGATCY 4 cut(s) 11, 69, 645, 1681
BstXI CCANNNNNNTGG 1 cut(s) 1576
BstYI RGATCY 4 cut(s) 11, 69, 645, 1681
Bsu15I ATCGAT 1 cut(s) 1281
Bsu36I CCTNAGG 1 cut(s) 1866
BsuI GTATCC 1 cut(s) 2454
BsuRI GGCC 8 cut(s) 59, 502, 572, 584, 593, 1251, 2289, 2568
BsuTUI ATCGAT 1 cut(s) 1281
BtgI CCRYGG 1 cut(s) 538
BtsI GCAGTG 3 cut(s) 1900, 1933, 2003
Cac8I GCNNGC 9 cut(s) 87, 434, 535, 547, 570, 1696, 1829, 2469, 2491
CciI TCATGA 1 cut(s) 1645
CfoI GCGC 3 cut(s) 242, 332, 1526
Cfr10I RCCGGY 2 cut(s) 1827, 2269
Cfr13I GGNCC 7 cut(s) 37, 57, 301, 766, 1286, 1766, 2600
Cfr9I CCCGGG 1 cut(s) 1256
ClaI ATCGAT 1 cut(s) 1281
CpoI CGGWCCG 1 cut(s) 766
CseI GACGC 3 cut(s) 691, 1306, 1595
CsiI ACCWGGT 1 cut(s) 2039
Csp6I GTAC 5 cut(s) 349, 558, 694, 895, 1384
CspI CGGWCCG 1 cut(s) 766
CviQI GTAC 5 cut(s) 349, 558, 694, 895, 1384
DdeI CTNAG 5 cut(s) 782, 1517, 1866, 1979, 2413
DraIII CACNNNGTG 2 cut(s) 2043, 2299
DrdI GACNNNNNNGTC 2 cut(s) 299, 341
DseDI GACNNNNNNGTC 2 cut(s) 299, 341
EaeI YGGCCR 2 cut(s) 582, 1249
Eam1104I CTCTTC 4 cut(s) 768, 1113, 1593, 2154
EarI CTCTTC 4 cut(s) 768, 1113, 1593, 2154
Ecl136II GAGCTC 2 cut(s) 1407, 2347
Eco130I CCWWGG 1 cut(s) 2060
Eco147I AGGCCT 3 cut(s) 572, 593, 2568
Eco24I GRGCYC 4 cut(s) 790, 1409, 1666, 2349
Eco31I GGTCTC 1 cut(s) 1205
Eco47I GGWCC 6 cut(s) 37, 301, 766, 1286, 1766, 2600
Eco53kI GAGCTC 2 cut(s) 1407, 2347
Eco57I CTGAAG 4 cut(s) 953, 1037, 1759, 1857
Eco81I CCTNAGG 1 cut(s) 1866
Eco88I CYCGRG 2 cut(s) 7, 1256
EcoICRI GAGCTC 2 cut(s) 1407, 2347
EcoO109I RGGNCCY 1 cut(s) 1766
EcoRII CCWGG 3 cut(s) 1541, 2039, 2220
EcoT14I CCWWGG 1 cut(s) 2060
EcoT22I ATGCAT 1 cut(s) 2399
EcoT38I GRGCYC 4 cut(s) 790, 1409, 1666, 2349
ErhI CCWWGG 1 cut(s) 2060
Esp3I CGTCTC 3 cut(s) 223, 243, 1518
FalI AAGNNNNNCTT 2 cut(s) 2220, 2252
FaqI GGGAC 1 cut(s) 1779
FauNDI CATATG 1 cut(s) 957
FbaI TGATCA 3 cut(s) 1479, 1675, 2239
Fnu4HI GCNGC 9 cut(s) 139, 322, 430, 521, 1109, 1311, 1446, 2111, 2234
FriOI GRGCYC 4 cut(s) 790, 1409, 1666, 2349
Fsp4HI GCNGC 9 cut(s) 139, 322, 430, 521, 1109, 1311, 1446, 2111, 2234
FspBI CTAG 2 cut(s) 2009, 2246
GlaI GCGC 3 cut(s) 241, 331, 1525
GluI GCNGC 9 cut(s) 139, 322, 430, 521, 1109, 1311, 1446, 2111, 2234
GsaI CCCAGC 3 cut(s) 869, 1172, 1726
HaeIII GGCC 8 cut(s) 59, 502, 572, 584, 593, 1251, 2289, 2568
HgaI GACGC 3 cut(s) 691, 1306, 1595
HhaI GCGC 3 cut(s) 242, 332, 1526
Hin1I GRCGYC 2 cut(s) 336, 399
Hin6I GCGC 3 cut(s) 240, 330, 1524
HinP1I GCGC 3 cut(s) 240, 330, 1524
HincII GTYRAC 1 cut(s) 463
HindII GTYRAC 1 cut(s) 463
HindIII AAGCTT 2 cut(s) 707, 1781
HphI GGTGA 4 cut(s) 1448, 2029, 2234, 2370
Hpy166II GTNNAC 8 cut(s) 463, 545, 560, 1386, 1879, 2046, 2174, 2323
Hpy8I GTNNAC 8 cut(s) 463, 545, 560, 1386, 1879, 2046, 2174, 2323
Hpy99I CGWCG 8 cut(s) 128, 341, 401, 404, 454, 742, 745, 1300
HpyAV CCTTC 5 cut(s) 604, 1003, 1012, 1135, 1963
HpyCH4III ACNGT 7 cut(s) 131, 542, 557, 1390, 2171, 2497, 2542
HpyCH4IV ACGT 7 cut(s) 180, 336, 351, 399, 576, 639, 1382
HpyF10VI GCNNNNNNNGC 4 cut(s) 327, 590, 1319, 1661
HpyF3I CTNAG 5 cut(s) 782, 1517, 1866, 1979, 2413
HpySE526I ACGT 7 cut(s) 180, 336, 351, 399, 576, 639, 1382
Hsp92I GRCGYC 2 cut(s) 336, 399
HspAI GCGC 3 cut(s) 240, 330, 1524
KflI GGGWCCC 1 cut(s) 1766
Kpn2I TCCGGA 1 cut(s) 2611
KroI GCCGGC 1 cut(s) 1827
KroNI GCCGGC 1 cut(s) 1829
Ksp22I TGATCA 3 cut(s) 1479, 1675, 2239
LguI GCTCTTC 1 cut(s) 1113
LmnI GCTCC 5 cut(s) 1404, 1891, 2078, 2122, 2344
Lsp1109I GCAGC 7 cut(s) 416, 507, 1120, 1297, 1457, 2097, 2220
LweI GCATC 9 cut(s) 1132, 1543, 1555, 1709, 1737, 2185, 2194, 2445, 2588
MabI ACCWGGT 1 cut(s) 2039
MaeI CTAG 2 cut(s) 2009, 2246
MaeII ACGT 7 cut(s) 180, 336, 351, 399, 576, 639, 1382
MaeIII GTNAC 3 cut(s) 1291, 2030, 2376
MflI RGATCY 4 cut(s) 11, 69, 645, 1681
MhlI GDGCHC 6 cut(s) 547, 790, 1409, 1666, 2176, 2349
MlsI TGGCCA 1 cut(s) 1251
MluCI AATT 6 cut(s) 31, 796, 1366, 1493, 1573, 1915
MluNI TGGCCA 1 cut(s) 1251
MlyI GAGTC 2 cut(s) 87, 378
MmeI TCCRAC 9 cut(s) 47, 147, 177, 246, 331, 723, 1407, 1572, 2075
Mox20I TGGCCA 1 cut(s) 1251
Mph1103I ATGCAT 1 cut(s) 2399
MroI TCCGGA 1 cut(s) 2611
MroNI GCCGGC 1 cut(s) 1827
MroXI GAANNNNTTC 1 cut(s) 777
MscI TGGCCA 1 cut(s) 1251
MseI TTAA 6 cut(s) 362, 933, 1074, 1344, 2280, 2430
MslI CAYNNNNRTG 2 cut(s) 1586, 2193
Msp20I TGGCCA 1 cut(s) 1251
MspA1I CMGCKG 1 cut(s) 429
MspR9I CCNGG 9 cut(s) 56, 626, 770, 1181, 1257, 1258, 1543, 2041, 2222
Mva1269I GAATGC 1 cut(s) 867
MvaI CCWGG 3 cut(s) 1543, 2041, 2222
MvnI CGCG 4 cut(s) 240, 242, 332, 702
MwoI GCNNNNNNNGC 4 cut(s) 327, 590, 1319, 1661
NaeI GCCGGC 1 cut(s) 1829
NciI CCSGG 6 cut(s) 56, 626, 770, 1181, 1257, 1258
NdeI CATATG 1 cut(s) 957
NgoMIV GCCGGC 1 cut(s) 1827
NlaIV GGNNCC 7 cut(s) 39, 71, 647, 1374, 1767, 1768, 2124
NmuCI GTSAC 2 cut(s) 1291, 2376
NsiI ATGCAT 1 cut(s) 2399
NspI RCATGY 1 cut(s) 520
NspV TTCGAA 1 cut(s) 279
PaeR7I CTCGAG 1 cut(s) 7
PagI TCATGA 1 cut(s) 1645
PceI AGGCCT 3 cut(s) 572, 593, 2568
PciSI GCTCTTC 1 cut(s) 1113
PcsI WCGNNNNNNNCGW 3 cut(s) 244, 384, 553
PctI GAATGC 1 cut(s) 867
PdiI GCCGGC 1 cut(s) 1829
PdmI GAANNNNTTC 1 cut(s) 777
PfeI GAWTC 9 cut(s) 210, 606, 760, 778, 952, 1746, 1973, 2054, 2450
PflFI GACNNNGTC 1 cut(s) 398
PflMI CCANNNNNTGG 3 cut(s) 270, 664, 2597
PfoI TCCNGGA 1 cut(s) 768
PkrI GCNGC 9 cut(s) 140, 323, 431, 522, 1110, 1312, 1447, 2112, 2235
PleI GAGTC 2 cut(s) 86, 377
PpsI GAGTC 2 cut(s) 86, 377
Ppu21I YACGTR 3 cut(s) 352, 577, 1383
PpuMI RGGWCCY 1 cut(s) 1766
PshAI GACNNNNGTC 1 cut(s) 455
Psp124BI GAGCTC 2 cut(s) 1409, 2349
Psp1406I AACGTT 1 cut(s) 180
Psp5II RGGWCCY 1 cut(s) 1766
Psp6I CCWGG 3 cut(s) 1541, 2039, 2220
PspFI CCCAGC 3 cut(s) 865, 1168, 1722
PspGI CCWGG 3 cut(s) 1541, 2039, 2220
PspN4I GGNNCC 7 cut(s) 39, 71, 647, 1374, 1767, 1768, 2124
PspPI GGNCC 7 cut(s) 37, 57, 301, 766, 1286, 1766, 2600
PspPPI RGGWCCY 1 cut(s) 1766
PsuI RGATCY 4 cut(s) 11, 69, 645, 1681
PsyI GACNNNGTC 1 cut(s) 398
RsaI GTAC 5 cut(s) 350, 559, 695, 896, 1385
RsaNI GTAC 5 cut(s) 349, 558, 694, 895, 1384
RseI CAYNNNNRTG 2 cut(s) 1586, 2193
Rsr2I CGGWCCG 1 cut(s) 766
RsrII CGGWCCG 1 cut(s) 766
SacI GAGCTC 2 cut(s) 1409, 2349
SapI GCTCTTC 1 cut(s) 1113
SaqAI TTAA 6 cut(s) 362, 933, 1074, 1344, 2280, 2430
SatI GCNGC 9 cut(s) 139, 322, 430, 521, 1109, 1311, 1446, 2111, 2234
Sau96I GGNCC 7 cut(s) 37, 57, 301, 766, 1286, 1766, 2600
SchI GAGTC 2 cut(s) 87, 378
ScrFI CCNGG 9 cut(s) 56, 626, 770, 1181, 1257, 1258, 1543, 2041, 2222
SduI GDGCHC 6 cut(s) 547, 790, 1409, 1666, 2176, 2349
SexAI ACCWGGT 1 cut(s) 2039
SfaNI GCATC 9 cut(s) 1132, 1543, 1555, 1709, 1737, 2185, 2194, 2445, 2588
SfcI CTRYAG 1 cut(s) 853
SfiI GGCCNNNNNGGCC 1 cut(s) 590
Sfr274I CTCGAG 1 cut(s) 7
SfuI TTCGAA 1 cut(s) 279
SinI GGWCC 6 cut(s) 37, 301, 766, 1286, 1766, 2600
SlaI CTCGAG 1 cut(s) 7
SmaI CCCGGG 1 cut(s) 1258
SmiMI CAYNNNNRTG 2 cut(s) 1586, 2193
SmlI CTYRAG 4 cut(s) 7, 682, 1132, 1685
SmoI CTYRAG 4 cut(s) 7, 682, 1132, 1685
Sse9I AATT 6 cut(s) 31, 796, 1366, 1493, 1573, 1915
SseBI AGGCCT 3 cut(s) 572, 593, 2568
SsiI CCGC 6 cut(s) 138, 321, 332, 427, 700, 2603
SspMI CTAG 2 cut(s) 2009, 2246
SstI GAGCTC 2 cut(s) 1409, 2349
StuI AGGCCT 3 cut(s) 572, 593, 2568
StyD4I CCNGG 9 cut(s) 54, 624, 768, 1179, 1255, 1256, 1541, 2039, 2220
StyI CCWWGG 1 cut(s) 2060
TaaI ACNGT 7 cut(s) 131, 542, 557, 1390, 2171, 2497, 2542
TaiI ACGT 7 cut(s) 183, 339, 354, 402, 579, 642, 1385
TaqII GACCGA 1 cut(s) 1897
TasI AATT 6 cut(s) 31, 796, 1366, 1493, 1573, 1915
TauI GCSGC 2 cut(s) 141, 324
TfiI GAWTC 9 cut(s) 210, 606, 760, 778, 952, 1746, 1973, 2054, 2450
Tru1I TTAA 6 cut(s) 362, 933, 1074, 1344, 2280, 2430
Tru9I TTAA 6 cut(s) 362, 933, 1074, 1344, 2280, 2430
TseFI GTSAC 2 cut(s) 1291, 2376
TseI GCWGC 7 cut(s) 429, 520, 1108, 1310, 1445, 2110, 2233
Tsp45I GTSAC 2 cut(s) 1291, 2376
TspDTI ATGAA 9 cut(s) 88, 806, 863, 944, 1118, 1379, 1586, 1939, 2620
TspGWI ACGGA 6 cut(s) 381, 467, 726, 1500, 1520, 2448
TspMI CCCGGG 1 cut(s) 1256
Tth111I GACNNNGTC 1 cut(s) 398
Van91I CCANNNNNTGG 3 cut(s) 270, 664, 2597
VneI GTGCAC 2 cut(s) 543, 2172
VpaK11BI GGWCC 6 cut(s) 37, 301, 766, 1286, 1766, 2600
XapI RAATTY 4 cut(s) 31, 1366, 1493, 1573
XceI RCATGY 1 cut(s) 520
XhoI CTCGAG 1 cut(s) 7
XmaI CCCGGG 1 cut(s) 1256
XmnI GAANNNNTTC 1 cut(s) 777
XspI CTAG 2 cut(s) 2009, 2246
ZraI GACGTC 2 cut(s) 337, 400
Zsp2I ATGCAT 1 cut(s) 2399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.