Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0017477

Physical Location & Seq

Genomic Coordinates Forward (+)

42595878 .. 42599642

Gene Structure 3765 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000008917

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2649 bp

ATGCTCTCGAGATCTTGCTTTGATTTACTGAATTTGGACCCCATTGACGATTTCCGGGCCGTCAGTCGGATCCCCAGAGTCATGCAAGCCCCCAGCTTCATCTCCGAGTTCAACAACAACTCCGACGACCGTTCCGGCGGCTCATCCTCATCCTCCTCCTCCTCCTCCTCATCCGACAACAACAACAACAACGTTGTTTCCTCTGTTTCGCAGGAACGAAGAATCATCGTCTCCAACCACCTGCCGATACGCACGTTTCGAGACGACGAGACGAACACGTGGCGTTTCGAATACGACCACGACAGCTTGGTCCTCCAACTCAAAGACGGCGGCGGCTTTGGCGCGGACGTCGAGGTCTTGTACGTGGGTTGCTTAAACGGAGCCGAGATCGACCCCTGCGATCAAGACGACGTCGCATTTGTCCTCCTCCACGAGTTCCACTGCGTGCCGTGTTTTTTACCGACGGAAGTCGTTAACAAGTATTATCACGGCTTCTGTAAGCACTATCTCTGGCCTCTGTTTCACTACATGCTGCCAATGTCAGCCAGCCACGGTGCACGCTTCGACCGTGCACTCTGGCAGGCCTACGTGTCGGCCAACAAGGCCTTCGCCGATAGAATCATAGAGGTTCTAAACCCGGATGAGGACTACGTTTGGATCCATGATTACCATCTCATGGTTTTGCCTACTTTCTTGAGGAAAAGGTACTACCGCGTCAAGCTTGGTTTCTTTCTGCATAGTCCTTTTCCGTCGTCGGAGATTTACCGGACGATTCCGGTCCGGGAGGAGATTCTGAGGGCTCTGTTGAATTGTGATCTTGTTGGGTTCCATATATTTGATTATGCGAGGCACTTCTTGTCTTGCTGTAGCAGAATGCTGGGTCTTCATTATGAGTTTAAAAGAGGGTACATGGGTTTGGAGTATTATGGTCGAATAGTGAGTATTAAGATGTTGCCTGTGGGGATTCATATGGGTCAGCTTCAGTCTGTTTTGTCGCTTGATGATACTGCCAAGAAAGTAAAGGTGTTGAAGGAAAAGTTTGAGGGGAAGATTGTGATTTTGGGGGTGGATGATTTGGACTTGTTTAAGGGGATTAGTTTGAAGTTTCTGGCAATGAAGCAGCTTTTGGAAGAGCATCAGAGTTTGAGGGGGAAGGTGGTTATGGTGCAGATAACAAACCCGGCACGAAGCCGAGGCAAGGATGTGCAGGAGGTTCTGAATGAGACCAGTGCTATTGCAAAAGAGATCAATGACGAGTATGGCCAACCCGGGTATCAGCCCCTTGTTGTCATCGATGGACCTGTGACGACGCAAGACAAAGCTGCATATTATGCCATTTCGGAGTGCTGTTTGGTTAATGCTGTGAGGGATGGGATGAATTTGGTGCCTTACACGTACACTGTTTGTAGGCAGGGGAGCTCTGTTTTGGACAAGGTGTTGGAGATTGGTGATGAGGCAGCAGATACGACACCTAAACCAAAAAAGAGTGTGATCATTGTCTCTGAATTTATTGGCTGTTCTCCGTCTCTTAGTGGCGCAATCCGTGTCAATCCCTGGAACATTGATGCTGTCTCTGATGCCATAAATTTGGTCATCACAATGTCGGAGGAAGAGAAGCGTCTTCGCCATGACAAACATTACAAGTATATCAGCTCTCATGATGTTGCTTATTGGGCTCGGAGTTTTGATCAAGATCTTGAGAGAGCTTGCAGAGAGCATTATCAGAGGAGATGCTGGGGCATCGGTTTTGGTTTGGGATTCAGAGTTGTAGCTTTGGGACCCAACTTCAGGAAGCTCTCTGTGGATCACATTGCCTATGCTTACAGAAATACAAGTAGCCGGCTCATCTTATTGGACTATGATGGCACTATGACACCTCAGGCTTCAGTGGACAAAGCTCCAAGCAGTGAAGTTATTTCGGTCTTGAATTGCTTATGCAGTGATCCCAAAAACATTGTTTTCATTGTGAGTGGCAGAGGGAAGGATTCTCTCAGCAAGTGGTTTTCGCAGTGTGAGAAACTAGGTTTATCAGCAGAGCATGGTTACTTCACCAGGTGGACTAAAGATTCTCCTTGGAAGACTTGCTCCTTGACAATGGATTTCAGTTGGAAGAAGATTGTGCTGCCTATTATGGAGCCATACACAGAAGCAACCGATGGTTCTTTTATAGAGGAGAAAGACAGTGCACTGGTCTGGCATCATCAAGATGCTGATCCTCATTTCGGCTCATCCCAGGCTAAAGAGCTGCTTGATCACCTAGAGAGTGTGCTTGTCAACGAGCCGGTTGTAGTTAAAAGAGGCCAACACATTGTGGAAGTGAAACCACTGGATGTGAACAAAGGTATAGTTGTGGAGGAGCTCATCTCCACTATGCGAAATAGGGTGAAGTCACCAGATTTCTTGTTATGCATAGGGGATGATCGCTCAGATGAGGATATGTTTAAGAGCATCATGTATCCCGAATCAAGTCCGTCCTTGCCAGCCATTGCAGAAGTCTTTGCTTGCACTGTTGGTCAAAAACCAAGCATGGCAAAATACTATTTGGATGATACAGTTGATGTCATCAAGTTGCTTCAAGGCCTCGCTTCAGCATCAGCGCAACCAAAGTCTGGACCGCAGTTTCCGGAAGCATTTGAAGTGTTCATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005975 GO:0005984 GO:0005991 GO:0005992 GO:0006793 GO:0006796 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009311 GO:0009312 GO:0009987 GO:0016051 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0033554 GO:0034637 GO:0042578 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044262 GO:0044424 GO:0044444 GO:0044464 GO:0046351 GO:0050896 GO:0051716 GO:0070413 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glyco_transf_20 Trehalose_PPase

Protein Analysis

883

Amino Acids

99.69

Weight (kDa)

5.78

Isoelectric Point (pI)

49.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_20	PF00982	73 - 567	4.6e-176	Glycosyltransferase family 20
Trehalose_PPase	PF02358	617 - 851	8e-81	Trehalose-phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017477)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G18700
fragaria_vesca	FvH4_4g09780
prunus_persica	Prupe.1G095500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0404641
rosa_laevigata	RLG00000008917
rosa_multiflora	Rmu_sc0002699.1_g000030
rosa_roxburghii	Rroxscaffold_5G00348790
rosa_rugosa	Rorug04G0048300
rosa_samantha	Rh4AG124100 Rh4BG118100 Rh4CG132200
rosa_wichuraiana	Rw4G010060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	249
AasI	GACNNNNNNGTC	2 cut(s)	308, 353
AatII	GACGTC	2 cut(s)	351, 414
Acc36I	ACCTGC	1 cut(s)	249
AccB1I	GGYRCC	1 cut(s)	1384
AccB7I	CCANNNNNTGG	2 cut(s)	676, 2609
AccII	CGCG	2 cut(s)	344, 714
AccIII	TCCGGA	1 cut(s)	2623
AciI	CCGC	6 cut(s)	138, 330, 333, 344, 712, 2615
AclI	AACGTT	1 cut(s)	192
AclWI	GGATC	7 cut(s)	64, 77, 652, 665, 1812, 1937, 2207
AcoI	YGGCCR	2 cut(s)	594, 1261
AcsI	RAATTY	4 cut(s)	31, 1378, 1505, 1585
AcuI	CTGAAG	4 cut(s)	965, 1771, 1869, 2571
AcvI	CACGTG	1 cut(s)	279
AcyI	GRCGYC	2 cut(s)	348, 411
AdeI	CACNNNGTG	3 cut(s)	445, 2055, 2311
AfaI	GTAC	4 cut(s)	362, 707, 908, 1397
AfiI	CCNNNNNNNGG	7 cut(s)	66, 643, 676, 1198, 1788, 2222, 2609
AflIII	ACRYGT	3 cut(s)	276, 588, 1392
AgsI	TTSAA	7 cut(s)	112, 808, 1030, 1102, 1927, 2576, 2636
AjnI	CCWGG	3 cut(s)	1553, 2051, 2232
AjuI	GAANNNNNNNTTGG	4 cut(s)	590, 622, 1109, 1141
Alw21I	GWGCWC	5 cut(s)	559, 574, 1421, 2188, 2361
Alw26I	GTCTC	7 cut(s)	235, 255, 263, 1217, 1504, 1530, 1576
Alw44I	GTGCAC	3 cut(s)	555, 570, 2184
AlwI	GGATC	7 cut(s)	64, 77, 652, 665, 1812, 1937, 2207
Ama87I	CYCGRG	2 cut(s)	7, 1268
Aor13HI	TCCGGA	1 cut(s)	2623
AoxI	GGCC	8 cut(s)	57, 512, 582, 594, 603, 1261, 2299, 2578
ApaLI	GTGCAC	3 cut(s)	555, 570, 2184
ApeKI	GCWGC	6 cut(s)	532, 1120, 1322, 1457, 2122, 2245
ApoI	RAATTY	4 cut(s)	31, 1378, 1505, 1585
ArsI	GACNNNNNNTTYG	2 cut(s)	401, 433
AspLEI	GCGC	3 cut(s)	344, 1538, 2599
AspS9I	GGNCC	7 cut(s)	37, 57, 310, 778, 1298, 1778, 2612
AsuC2I	CCSGG	6 cut(s)	56, 638, 782, 1181, 1269, 1270
AsuHPI	GGTGA	5 cut(s)	1460, 2041, 2246, 2382, 2395
AsuII	TTCGAA	1 cut(s)	288
AvaI	CYCGRG	2 cut(s)	7, 1268
AvaII	GGWCC	6 cut(s)	37, 310, 778, 1298, 1778, 2612
AxyI	CCTNAGG	1 cut(s)	1878
BaeGI	GKGCMC	3 cut(s)	559, 574, 2188
BaeI	ACNNNNGTAYC	6 cut(s)	239, 272, 898, 931, 1256, 1289
BalI	TGGCCA	1 cut(s)	1263
BamHI	GGATCC	2 cut(s)	69, 657
BanI	GGYRCC	1 cut(s)	1384
BanII	GRGCYC	4 cut(s)	802, 1421, 1678, 2361
BauI	CACGAG	1 cut(s)	431
BbrPI	CACGTG	1 cut(s)	279
BbsI	GAAGAC	3 cut(s)	875, 1613, 2084
Bbv12I	GWGCWC	5 cut(s)	559, 574, 1421, 2188, 2361
BbvI	GCAGC	6 cut(s)	519, 1132, 1309, 1469, 2109, 2232
BccI	CCATC	5 cut(s)	678, 1289, 1364, 1856, 2150
BceAI	ACGGC	4 cut(s)	44, 343, 433, 505
BciT130I	CCWGG	3 cut(s)	1555, 2053, 2234
BciVI	GTATCC	1 cut(s)	2466
BclI	TGATCA	3 cut(s)	1491, 1687, 2251
BcnI	CCSGG	6 cut(s)	56, 638, 782, 1181, 1269, 1270
BcoDI	GTCTC	7 cut(s)	235, 255, 263, 1217, 1504, 1530, 1576
BfaI	CTAG	2 cut(s)	2021, 2258
BfmI	CTRYAG	1 cut(s)	865
BfuAI	ACCTGC	1 cut(s)	249
BfuI	GTATCC	1 cut(s)	2466
BglI	GCCNNNNNGGC	1 cut(s)	602
BglII	AGATCT	2 cut(s)	11, 1693
BisI	GCNGC	9 cut(s)	139, 331, 334, 533, 1121, 1323, 1458, 2123, 2246
BlsI	GCNGC	9 cut(s)	140, 332, 335, 534, 1122, 1324, 1459, 2124, 2247
Bme1390I	CCNGG	9 cut(s)	56, 638, 782, 1181, 1269, 1270, 1555, 2053, 2234
Bme18I	GGWCC	6 cut(s)	37, 310, 778, 1298, 1778, 2612
BmeT110I	CYCGRG	2 cut(s)	7, 1268
BmgT120I	GGNCC	7 cut(s)	37, 57, 310, 778, 1298, 1778, 2612
BmiI	GGNNCC	9 cut(s)	39, 71, 382, 659, 827, 1386, 1779, 1780, 2136
BmrFI	CCNGG	9 cut(s)	56, 638, 782, 1181, 1269, 1270, 1555, 2053, 2234
BmsI	GCATC	9 cut(s)	1144, 1555, 1567, 1721, 1749, 2197, 2206, 2457, 2600
BoxI	GACNNNNGTC	1 cut(s)	467
BpiI	GAAGAC	3 cut(s)	875, 1613, 2084
BplI	GAGNNNNNCTC	2 cut(s)	2348, 2380
Bpu14I	TTCGAA	1 cut(s)	288
BpuEI	CTTGAG	2 cut(s)	715, 1718
BpuMI	CCSGG	6 cut(s)	56, 638, 782, 1181, 1269, 1270
Bsa29I	ATCGAT	1 cut(s)	1293
BsaAI	YACGTR	4 cut(s)	279, 364, 589, 1395
BsaBI	GATNNNNATC	3 cut(s)	669, 1692, 2211
BsaHI	GRCGYC	2 cut(s)	348, 411
BsaI	GGTCTC	1 cut(s)	1217
BsaJI	CCNNGG	6 cut(s)	550, 1192, 1268, 1553, 2072, 2232
BsaWI	WCCGGW	3 cut(s)	765, 775, 2623
BsaXI	ACNNNNNCTCC	2 cut(s)	104, 134
Bsc4I	CCNNNNNNNGG	7 cut(s)	66, 643, 676, 1198, 1788, 2222, 2609
Bse118I	RCCGGY	2 cut(s)	1839, 2281
Bse1I	ACTGG	3 cut(s)	1227, 2193, 2331
Bse21I	CCTNAGG	1 cut(s)	1878
Bse3DI	GCAATG	3 cut(s)	1119, 1809, 2484
Bse8I	GATNNNNATC	3 cut(s)	669, 1692, 2211
BseAI	TCCGGA	1 cut(s)	2623
BseBI	CCWGG	3 cut(s)	1555, 2053, 2234
BseCI	ATCGAT	1 cut(s)	1293
BseDI	CCNNGG	6 cut(s)	550, 1192, 1268, 1553, 2072, 2232
BseJI	GATNNNNATC	3 cut(s)	669, 1692, 2211
BseLI	CCNNNNNNNGG	7 cut(s)	66, 643, 676, 1198, 1788, 2222, 2609
BseMI	GCAATG	3 cut(s)	1119, 1809, 2484
BseMII	CTCAG	4 cut(s)	785, 1892, 2005, 2439
BseNI	ACTGG	3 cut(s)	1227, 2193, 2331
BseSI	GKGCMC	3 cut(s)	559, 574, 2188
BseXI	GCAGC	6 cut(s)	519, 1132, 1309, 1469, 2109, 2232
BseYI	CCCAGC	3 cut(s)	92, 877, 1734
BsgI	GTGCAG	2 cut(s)	1187, 1226
Bsh1236I	CGCG	2 cut(s)	344, 714
Bsh1285I	CGRYCG	2 cut(s)	130, 568
BshFI	GGCC	8 cut(s)	59, 514, 584, 596, 605, 1263, 2301, 2580
BshNI	GGYRCC	1 cut(s)	1384
BshVI	ATCGAT	1 cut(s)	1293
BsiEI	CGRYCG	2 cut(s)	130, 568
BsiHKAI	GWGCWC	5 cut(s)	559, 574, 1421, 2188, 2361
BsiHKCI	CYCGRG	2 cut(s)	7, 1268
BslFI	GGGAC	1 cut(s)	1791
BslI	CCNNNNNNNGG	7 cut(s)	66, 643, 676, 1198, 1788, 2222, 2609
BsmAI	GTCTC	7 cut(s)	235, 255, 263, 1217, 1504, 1530, 1576
BsmBI	CGTCTC	4 cut(s)	235, 255, 263, 1530
BsmFI	GGGAC	1 cut(s)	1791
BsmI	GAATGC	1 cut(s)	879
BsnI	GGCC	8 cut(s)	59, 514, 584, 596, 605, 1263, 2301, 2580
Bso31I	GGTCTC	1 cut(s)	1217
BsoBI	CYCGRG	2 cut(s)	7, 1268
Bsp119I	TTCGAA	1 cut(s)	288
Bsp1286I	GDGCHC	7 cut(s)	559, 574, 802, 1421, 1678, 2188, 2361
Bsp13I	TCCGGA	1 cut(s)	2623
BspACI	CCGC	6 cut(s)	138, 330, 333, 344, 712, 2615
BspANI	GGCC	8 cut(s)	59, 514, 584, 596, 605, 1263, 2301, 2580
BspCNI	CTCAG	4 cut(s)	786, 1891, 2004, 2438
BspDI	ATCGAT	1 cut(s)	1293
BspEI	TCCGGA	1 cut(s)	2623
BspFNI	CGCG	2 cut(s)	344, 714
BspHI	TCATGA	1 cut(s)	1657
BspLI	GGNNCC	9 cut(s)	39, 71, 382, 659, 827, 1386, 1779, 1780, 2136
BspMI	ACCTGC	1 cut(s)	249
BspPI	GGATC	7 cut(s)	64, 77, 652, 665, 1812, 1937, 2207
BspQI	GCTCTTC	1 cut(s)	1125
BspT104I	TTCGAA	1 cut(s)	288
BspT107I	GGYRCC	1 cut(s)	1384
BspTNI	GGTCTC	1 cut(s)	1217
BsrDI	GCAATG	3 cut(s)	1119, 1809, 2484
BsrFI	RCCGGY	2 cut(s)	1839, 2281
BsrI	ACTGG	3 cut(s)	1227, 2193, 2331
BssAI	RCCGGY	2 cut(s)	1839, 2281
BssECI	CCNNGG	6 cut(s)	550, 1192, 1268, 1553, 2072, 2232
BssNI	GRCGYC	2 cut(s)	348, 411
BssSI	CACGAG	1 cut(s)	431
BssT1I	CCWWGG	1 cut(s)	2072
Bst2BI	CACGAG	1 cut(s)	431
Bst2UI	CCWGG	3 cut(s)	1555, 2053, 2234
Bst4CI	ACNGT	7 cut(s)	131, 554, 569, 1402, 2183, 2509, 2554
Bst6I	CTCTTC	2 cut(s)	1125, 1605
BstACI	GRCGYC	2 cut(s)	348, 411
BstAPI	GCANNNNNTGC	1 cut(s)	1331
BstBAI	YACGTR	4 cut(s)	279, 364, 589, 1395
BstBI	TTCGAA	1 cut(s)	288
BstC8I	GCNNGC	9 cut(s)	87, 446, 547, 559, 582, 1708, 1841, 2481, 2503
BstDEI	CTNAG	5 cut(s)	794, 1529, 1878, 1991, 2425
BstDSI	CCRYGG	1 cut(s)	550
BstFNI	CGCG	2 cut(s)	344, 714
BstHHI	GCGC	3 cut(s)	344, 1538, 2599
BstMAI	GTCTC	7 cut(s)	235, 255, 263, 1217, 1504, 1530, 1576
BstMCI	CGRYCG	2 cut(s)	130, 568
BstMWI	GCNNNNNNNGC	4 cut(s)	339, 602, 1331, 1673
BstNI	CCWGG	3 cut(s)	1555, 2053, 2234
BstNSI	RCATGY	1 cut(s)	532
BstPAI	GACNNNNGTC	1 cut(s)	467
BstSCI	CCNGG	9 cut(s)	54, 636, 780, 1179, 1267, 1268, 1553, 2051, 2232
BstSFI	CTRYAG	1 cut(s)	865
BstSLI	GKGCMC	3 cut(s)	559, 574, 2188
BstUI	CGCG	2 cut(s)	344, 714
BstV1I	GCAGC	6 cut(s)	519, 1132, 1309, 1469, 2109, 2232
BstV2I	GAAGAC	3 cut(s)	875, 1613, 2084
BstX2I	RGATCY	4 cut(s)	11, 69, 657, 1693
BstXI	CCANNNNNNTGG	1 cut(s)	1588
BstYI	RGATCY	4 cut(s)	11, 69, 657, 1693
Bsu15I	ATCGAT	1 cut(s)	1293
Bsu36I	CCTNAGG	1 cut(s)	1878
BsuI	GTATCC	1 cut(s)	2466
BsuRI	GGCC	8 cut(s)	59, 514, 584, 596, 605, 1263, 2301, 2580
BsuTUI	ATCGAT	1 cut(s)	1293
BtgI	CCRYGG	1 cut(s)	550
BtsI	GCAGTG	4 cut(s)	439, 1912, 1945, 2015
BveI	ACCTGC	1 cut(s)	249
Cac8I	GCNNGC	9 cut(s)	87, 446, 547, 559, 582, 1708, 1841, 2481, 2503
CciI	TCATGA	1 cut(s)	1657
CfoI	GCGC	3 cut(s)	344, 1538, 2599
Cfr10I	RCCGGY	2 cut(s)	1839, 2281
Cfr13I	GGNCC	7 cut(s)	37, 57, 310, 778, 1298, 1778, 2612
Cfr9I	CCCGGG	1 cut(s)	1268
ClaI	ATCGAT	1 cut(s)	1293
CpoI	CGGWCCG	1 cut(s)	778
CseI	GACGC	3 cut(s)	703, 1318, 1607
CsiI	ACCWGGT	1 cut(s)	2051
Csp6I	GTAC	4 cut(s)	361, 706, 907, 1396
CspI	CGGWCCG	1 cut(s)	778
CviQI	GTAC	4 cut(s)	361, 706, 907, 1396
DdeI	CTNAG	5 cut(s)	794, 1529, 1878, 1991, 2425
DraI	TTTAAA	1 cut(s)	898
DraIII	CACNNNGTG	3 cut(s)	445, 2055, 2311
DrdI	GACNNNNNNGTC	2 cut(s)	308, 353
DseDI	GACNNNNNNGTC	2 cut(s)	308, 353
EaeI	YGGCCR	2 cut(s)	594, 1261
Eam1104I	CTCTTC	2 cut(s)	1125, 1605
EarI	CTCTTC	2 cut(s)	1125, 1605
Ecl136II	GAGCTC	2 cut(s)	1419, 2359
Eco130I	CCWWGG	1 cut(s)	2072
Eco147I	AGGCCT	3 cut(s)	584, 605, 2580
Eco24I	GRGCYC	4 cut(s)	802, 1421, 1678, 2361
Eco31I	GGTCTC	1 cut(s)	1217
Eco47I	GGWCC	6 cut(s)	37, 310, 778, 1298, 1778, 2612
Eco53kI	GAGCTC	2 cut(s)	1419, 2359
Eco57I	CTGAAG	4 cut(s)	965, 1771, 1869, 2571
Eco72I	CACGTG	1 cut(s)	279
Eco81I	CCTNAGG	1 cut(s)	1878
Eco88I	CYCGRG	2 cut(s)	7, 1268
EcoICRI	GAGCTC	2 cut(s)	1419, 2359
EcoO109I	RGGNCCY	1 cut(s)	1778
EcoRII	CCWGG	3 cut(s)	1553, 2051, 2232
EcoT14I	CCWWGG	1 cut(s)	2072
EcoT22I	ATGCAT	1 cut(s)	2411
EcoT38I	GRGCYC	4 cut(s)	802, 1421, 1678, 2361
ErhI	CCWWGG	1 cut(s)	2072
Esp3I	CGTCTC	4 cut(s)	235, 255, 263, 1530
FalI	AAGNNNNNCTT	2 cut(s)	2232, 2264
FaqI	GGGAC	1 cut(s)	1791
FauNDI	CATATG	1 cut(s)	969
FbaI	TGATCA	3 cut(s)	1491, 1687, 2251
Fnu4HI	GCNGC	9 cut(s)	139, 331, 334, 533, 1121, 1323, 1458, 2123, 2246
FriOI	GRGCYC	4 cut(s)	802, 1421, 1678, 2361
Fsp4HI	GCNGC	9 cut(s)	139, 331, 334, 533, 1121, 1323, 1458, 2123, 2246
FspBI	CTAG	2 cut(s)	2021, 2258
GlaI	GCGC	3 cut(s)	343, 1537, 2598
GluI	GCNGC	9 cut(s)	139, 331, 334, 533, 1121, 1323, 1458, 2123, 2246
GsaI	CCCAGC	3 cut(s)	96, 881, 1738
HaeIII	GGCC	8 cut(s)	59, 514, 584, 596, 605, 1263, 2301, 2580
HgaI	GACGC	3 cut(s)	703, 1318, 1607
HhaI	GCGC	3 cut(s)	344, 1538, 2599
Hin1I	GRCGYC	2 cut(s)	348, 411
Hin6I	GCGC	3 cut(s)	342, 1536, 2597
HinP1I	GCGC	3 cut(s)	342, 1536, 2597
HincII	GTYRAC	2 cut(s)	475, 2275
HindII	GTYRAC	2 cut(s)	475, 2275
HindIII	AAGCTT	1 cut(s)	719
HpaI	GTTAAC	1 cut(s)	475
HphI	GGTGA	5 cut(s)	1460, 2041, 2246, 2382, 2395
Hpy166II	GTNNAC	9 cut(s)	475, 557, 572, 1398, 1891, 2058, 2186, 2275, 2335
Hpy8I	GTNNAC	9 cut(s)	475, 557, 572, 1398, 1891, 2058, 2186, 2275, 2335
Hpy99I	CGWCG	9 cut(s)	128, 269, 353, 413, 416, 466, 754, 757, 1312
HpyAV	CCTTC	4 cut(s)	616, 1024, 1147, 1975
HpyCH4III	ACNGT	7 cut(s)	131, 554, 569, 1402, 2183, 2509, 2554
HpyCH4IV	ACGT	9 cut(s)	192, 254, 278, 348, 363, 411, 588, 651, 1394
HpyF10VI	GCNNNNNNNGC	4 cut(s)	339, 602, 1331, 1673
HpyF3I	CTNAG	5 cut(s)	794, 1529, 1878, 1991, 2425
HpySE526I	ACGT	9 cut(s)	192, 254, 278, 348, 363, 411, 588, 651, 1394
Hsp92I	GRCGYC	2 cut(s)	348, 411
HspAI	GCGC	3 cut(s)	342, 1536, 2597
KflI	GGGWCCC	1 cut(s)	1778
Kpn2I	TCCGGA	1 cut(s)	2623
KroI	GCCGGC	1 cut(s)	1839
KroNI	GCCGGC	1 cut(s)	1841
Ksp22I	TGATCA	3 cut(s)	1491, 1687, 2251
KspAI	GTTAAC	1 cut(s)	475
LguI	GCTCTTC	1 cut(s)	1125
LmnI	GCTCC	6 cut(s)	380, 1416, 1903, 2090, 2134, 2356
Lsp1109I	GCAGC	6 cut(s)	519, 1132, 1309, 1469, 2109, 2232
LweI	GCATC	9 cut(s)	1144, 1555, 1567, 1721, 1749, 2197, 2206, 2457, 2600
MabI	ACCWGGT	1 cut(s)	2051
MaeI	CTAG	2 cut(s)	2021, 2258
MaeII	ACGT	9 cut(s)	192, 254, 278, 348, 363, 411, 588, 651, 1394
MaeIII	GTNAC	3 cut(s)	1303, 2042, 2388
MboII	GAAGA	9 cut(s)	231, 875, 1060, 1142, 1613, 1622, 2089, 2122, 2125
MflI	RGATCY	4 cut(s)	11, 69, 657, 1693
MhlI	GDGCHC	7 cut(s)	559, 574, 802, 1421, 1678, 2188, 2361
MlsI	TGGCCA	1 cut(s)	1263
MluCI	AATT	6 cut(s)	31, 808, 1378, 1505, 1585, 1927
MluNI	TGGCCA	1 cut(s)	1263
MlyI	GAGTC	1 cut(s)	87
MmeI	TCCRAC	9 cut(s)	47, 147, 198, 258, 340, 735, 1419, 1584, 2087
Mox20I	TGGCCA	1 cut(s)	1263
Mph1103I	ATGCAT	1 cut(s)	2411
MroI	TCCGGA	1 cut(s)	2623
MroNI	GCCGGC	1 cut(s)	1839
MscI	TGGCCA	1 cut(s)	1263
MseI	TTAA	8 cut(s)	374, 474, 897, 945, 1086, 1356, 2292, 2442
MslI	CAYNNNNRTG	2 cut(s)	1598, 2205
Msp20I	TGGCCA	1 cut(s)	1263
MspR9I	CCNGG	9 cut(s)	56, 638, 782, 1181, 1269, 1270, 1555, 2053, 2234
Mva1269I	GAATGC	1 cut(s)	879
MvaI	CCWGG	3 cut(s)	1555, 2053, 2234
MvnI	CGCG	2 cut(s)	344, 714
MwoI	GCNNNNNNNGC	4 cut(s)	339, 602, 1331, 1673
NaeI	GCCGGC	1 cut(s)	1841
NciI	CCSGG	6 cut(s)	56, 638, 782, 1181, 1269, 1270
NdeI	CATATG	1 cut(s)	969
NgoMIV	GCCGGC	1 cut(s)	1839
NlaIV	GGNNCC	9 cut(s)	39, 71, 382, 659, 827, 1386, 1779, 1780, 2136
NmeAIII	GCCGAG	2 cut(s)	409, 1217
NmuCI	GTSAC	2 cut(s)	1303, 2388
NsiI	ATGCAT	1 cut(s)	2411
NspI	RCATGY	1 cut(s)	532
NspV	TTCGAA	1 cut(s)	288
PaeR7I	CTCGAG	1 cut(s)	7
PagI	TCATGA	1 cut(s)	1657
PaqCI	CACCTGC	1 cut(s)	249
PceI	AGGCCT	3 cut(s)	584, 605, 2580
PciSI	GCTCTTC	1 cut(s)	1125
PcsI	WCGNNNNNNNCGW	3 cut(s)	256, 396, 565
PctI	GAATGC	1 cut(s)	879
PdiI	GCCGGC	1 cut(s)	1841
PfeI	GAWTC	9 cut(s)	222, 618, 772, 790, 964, 1758, 1985, 2066, 2462
PflFI	GACNNNGTC	1 cut(s)	410
PflMI	CCANNNNNTGG	2 cut(s)	676, 2609
PfoI	TCCNGGA	1 cut(s)	780
PkrI	GCNGC	9 cut(s)	140, 332, 335, 534, 1122, 1324, 1459, 2124, 2247
PleI	GAGTC	1 cut(s)	86
PmaCI	CACGTG	1 cut(s)	279
PmlI	CACGTG	1 cut(s)	279
PpsI	GAGTC	1 cut(s)	86
Ppu21I	YACGTR	4 cut(s)	279, 364, 589, 1395
PpuMI	RGGWCCY	1 cut(s)	1778
PshAI	GACNNNNGTC	1 cut(s)	467
Psp124BI	GAGCTC	2 cut(s)	1421, 2361
Psp1406I	AACGTT	1 cut(s)	192
Psp5II	RGGWCCY	1 cut(s)	1778
Psp6I	CCWGG	3 cut(s)	1553, 2051, 2232
PspCI	CACGTG	1 cut(s)	279
PspFI	CCCAGC	3 cut(s)	92, 877, 1734
PspGI	CCWGG	3 cut(s)	1553, 2051, 2232
PspN4I	GGNNCC	9 cut(s)	39, 71, 382, 659, 827, 1386, 1779, 1780, 2136
PspPI	GGNCC	7 cut(s)	37, 57, 310, 778, 1298, 1778, 2612
PspPPI	RGGWCCY	1 cut(s)	1778
PsuI	RGATCY	4 cut(s)	11, 69, 657, 1693
PsyI	GACNNNGTC	1 cut(s)	410
RsaI	GTAC	4 cut(s)	362, 707, 908, 1397
RsaNI	GTAC	4 cut(s)	361, 706, 907, 1396
RseI	CAYNNNNRTG	2 cut(s)	1598, 2205
Rsr2I	CGGWCCG	1 cut(s)	778
RsrII	CGGWCCG	1 cut(s)	778
SacI	GAGCTC	2 cut(s)	1421, 2361
SapI	GCTCTTC	1 cut(s)	1125
SaqAI	TTAA	8 cut(s)	374, 474, 897, 945, 1086, 1356, 2292, 2442
SatI	GCNGC	9 cut(s)	139, 331, 334, 533, 1121, 1323, 1458, 2123, 2246
Sau96I	GGNCC	7 cut(s)	37, 57, 310, 778, 1298, 1778, 2612
SchI	GAGTC	1 cut(s)	87
ScrFI	CCNGG	9 cut(s)	56, 638, 782, 1181, 1269, 1270, 1555, 2053, 2234
SduI	GDGCHC	7 cut(s)	559, 574, 802, 1421, 1678, 2188, 2361
SexAI	ACCWGGT	1 cut(s)	2051
SfaNI	GCATC	9 cut(s)	1144, 1555, 1567, 1721, 1749, 2197, 2206, 2457, 2600
SfcI	CTRYAG	1 cut(s)	865
SfiI	GGCCNNNNNGGCC	1 cut(s)	602
Sfr274I	CTCGAG	1 cut(s)	7
SfuI	TTCGAA	1 cut(s)	288
SinI	GGWCC	6 cut(s)	37, 310, 778, 1298, 1778, 2612
SlaI	CTCGAG	1 cut(s)	7
SmaI	CCCGGG	1 cut(s)	1270
SmiMI	CAYNNNNRTG	2 cut(s)	1598, 2205
SmlI	CTYRAG	3 cut(s)	7, 694, 1697
SmoI	CTYRAG	3 cut(s)	7, 694, 1697
Sse9I	AATT	6 cut(s)	31, 808, 1378, 1505, 1585, 1927
SseBI	AGGCCT	3 cut(s)	584, 605, 2580
SsiI	CCGC	6 cut(s)	138, 330, 333, 344, 712, 2615
SspMI	CTAG	2 cut(s)	2021, 2258
SstI	GAGCTC	2 cut(s)	1421, 2361
StuI	AGGCCT	3 cut(s)	584, 605, 2580
StyD4I	CCNGG	9 cut(s)	54, 636, 780, 1179, 1267, 1268, 1553, 2051, 2232
StyI	CCWWGG	1 cut(s)	2072
TaaI	ACNGT	7 cut(s)	131, 554, 569, 1402, 2183, 2509, 2554
TaiI	ACGT	9 cut(s)	195, 257, 281, 351, 366, 414, 591, 654, 1397
TaqI	TCGA	8 cut(s)	8, 259, 288, 351, 390, 564, 931, 1293
TaqII	GACCGA	1 cut(s)	1909
TasI	AATT	6 cut(s)	31, 808, 1378, 1505, 1585, 1927
TauI	GCSGC	3 cut(s)	141, 333, 336
TfiI	GAWTC	9 cut(s)	222, 618, 772, 790, 964, 1758, 1985, 2066, 2462
Tru1I	TTAA	8 cut(s)	374, 474, 897, 945, 1086, 1356, 2292, 2442
Tru9I	TTAA	8 cut(s)	374, 474, 897, 945, 1086, 1356, 2292, 2442
TseFI	GTSAC	2 cut(s)	1303, 2388
TseI	GCWGC	6 cut(s)	532, 1120, 1322, 1457, 2122, 2245
Tsp45I	GTSAC	2 cut(s)	1303, 2388
TspDTI	ATGAA	7 cut(s)	88, 875, 956, 1130, 1391, 1951, 2632
TspGWI	ACGGA	6 cut(s)	393, 479, 738, 1512, 1532, 2460
TspMI	CCCGGG	1 cut(s)	1268
Tth111I	GACNNNGTC	1 cut(s)	410
Van91I	CCANNNNNTGG	2 cut(s)	676, 2609
VneI	GTGCAC	3 cut(s)	555, 570, 2184
VpaK11BI	GGWCC	6 cut(s)	37, 310, 778, 1298, 1778, 2612
XapI	RAATTY	4 cut(s)	31, 1378, 1505, 1585
XceI	RCATGY	1 cut(s)	532
XhoI	CTCGAG	1 cut(s)	7
XmaI	CCCGGG	1 cut(s)	1268
XspI	CTAG	2 cut(s)	2021, 2258
ZraI	GACGTC	2 cut(s)	349, 412
Zsp2I	ATGCAT	1 cut(s)	2411

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.