Rw4G010060

Glycosyltransferase family 20

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0017477
Physical Location & Seq
Reverse (-)
22991912 .. 22994925
3014 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G010060.1

Sequence Viewer

Length: 2556 bp
ATGCTCTCGAGATCTTGCTTTGATTTGCTGAATTTGGACCCCATTGACGATTTCCGGGCCGTCAGTCGGATCCCCAGAGTCATGCAAGCCTCCGGCTTCATCTCCGAGTTCGACAACAACTCCGACGACCGTTCCGGCGGCTCATCCTCATCCTCATCCAACAACAACAACGTTGTTTCCACTGTTTCGCAGGAACGAAGAATCATCGTCTCCAACCACTTGCCAATACGTGCGTTTCGAGACGAAGACGAAACCAACAAGTGGCGTTTCGAATACGACCACGACAGCTTGGTCCTCCAACTCAAAGACGGCGGCTTTGGCGCGGACGTCGAGGTCTTGTACGTGGGTTGCTTAAACGGAGTCGAGATCGACCCCTGCGATCAAGACGACGTCGCATTTGTCCTCCTCCACGAGTTCCGCTGCGTGCCGTGTTTTCTACCGACGGACGTCGTCAACAAGTTTTATCACGGCTTCTGTAAGCACTATCTTTGGCCTCTGTTTCACTACATGCTGCCCATGTCAGCCAGCCACGGTGCACGCTTCGACCGTACACTCTGGCAGGCCTACGTGTCGGCCAACAAGGCCTTCGCCGATAGAATCATAGAGGTTCTAAACCCGGATGAGGACTACGTTTGGATCCATGACTACCATCTCATGGTTTTGCCTACTTTCTTGAGGAAAAGGTACTACCGCGTCAAGCTTGGTTTCTTTCTGCATAGTCCTTTTCCGTCGTCGGAGATTTACCGGACGATTCCGGTCCGGGAAGAGATTCTGAGGGCTCTGTTGAATTGTGATCTTATTGGGTTCCATATATTTGATTATGCGAGGCACTTCTTGTCCTGCTGTAGCAGAATGCTGGGTCTTCATTATGAGTTCAAAAGAGGGTACATGGGTTTGGAGTATTATGGTCGAATAGTGAGTATTAAGATGTTGCCTGTGGGGATTCATATGGGTCAGCTTCAGTCTGTTTTGTCGCTTGATGATACTGCCAAGAAAGTGAAGGTGTTGAAGGAAAAGTTTGAGGGGAAGATTGTGATTTTGGGGGTGGATGATTTGGACTTGTTTAAGGGGATTAGTTTGAAGTTTCTGGCAATGAAGCAGCTTTTGGAAGAGCATCAGAGTTTGAGGGGAAGCCGAGGCAAGGATGTGCAGGAGGTTCTGAATGAGACCAGTGCTATTGCAAAAGAGATCAATGACAAGTACGGCCAACCCGGGTATCAGCCCCTTGTTGTCATCGATGGGCCTGTGACGACGCAAGACAAAGCTGCATATTATGCCATTTCGGAGTGCTGTTTGGTTAATGCTGTGAGGGATGGGATGAATTTGGTGCCTTACACTTACACTGTTTGTAGGCAGGGGAGCTCTGTTTTGGACAAGGTGTTGGAGATTGGTGATGAGGCAGCAGATACGACACCTAAACCGAAAAAGAGTGTGATCATTGTCTCTGAATTTATTGGCTGTTCTCCGTCTCTTAGTGGCGCAATCCGTGTCAATCCCTGGAACATTGATGCTCTCTCTGATGCCATGAATTTGGTCATCACAATGTCGGAGGAAGAGAAGCGTCTTCGCCATGACAAACATTACAAGTATATCAGCTCTCATGATGTTGCTTATTGGGCTCGACATTATCGGAGGAGATGCTGGGGCATCGGTTTAGGTTTGGGATTCAGAGTTGTAGCTTTGGGACCCAACTTCAGGAAGCTTTCTGTGGATCACATTGCCTATGCTTACAGAAATACAAGTAGCCGGCTCATCTTATTGGACTATGACGGCACTATGACACCTCAGGCTTCAGTGGACAAAGCTCCAAGCAGTGAAGTTATTTCGGTCTTGAATTGCTTATGCAGTGATCCGAAAAACATTGTTTTCATTGTGAGTGGCAGAGGGAAGGATTCTCTCAGCAAGTGGTTTTCGCAGTGTGAGAAACTAGGTTTATCAGCAGAGCATGGTTACTTCACCAGGTGGACTAAAGATTCTCCTTGGAAGACTTGCTCCTTGACAATGGATTTCAGTTGGAAGAAGATTGTGCTGCCTATTATGGAGCCTTACACAGAAGCAACCGATGGTTCTTTTATAGAAGAGAAAGACAGTGCACTGGTCTGGCATCATCAAGATGCTGATCCTCATTTCGGCTCATCCCAGGCTAAAGAGCTGCTTGATCACCTAGAGAGTGTGCTTGTCAATGAGCCGGTTGTAGTTAAAAGAGGCCAACACATTGTGGAAGTGAAACCACTGGATGTGAACAAAGGTATAGTTGTGGAGGAGCTCATCTCCACTATGCGAAATAGGGTGAAGTCACCAGATTTCTTGTTATGCATAGGGGATGATCGCTCAGATGAGGATATGTTTAAGAGCATCATGTATCCCGAATCAAGTCCGTCCTTGCCAGCCATTGCAGAAGTCTTTGCTTGCACTGTTGGTCAAAAACCAAGCATGGCAAAATACTATCTGGATGATACAGTTGATGTCATCAAGTTGCTTCAAGGCCTTGCTTCAGCAGCGGCACAACCAAAGTCTGGACCGCAGTTTCCGGAAGCATTTGAAGTGTTCATTTGA

Protein Analysis

851

Amino Acids

96.36

Weight (kDa)

5.93

Isoelectric Point (pI)

48.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_20 PF00982 66 - 544 1.9e-161 Glycosyltransferase family 20
Trehalose_PPase PF02358 586 - 820 7.5e-81 Trehalose-phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18700
fragaria_vesca FvH4_4g09780
prunus_persica Prupe.1G095500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0404641
rosa_laevigata RLG00000008917
rosa_multiflora Rmu_sc0002699.1_g000030
rosa_roxburghii Rroxscaffold_5G00348790
rosa_rugosa Rorug04G0048300
rosa_samantha Rh4AG124100 Rh4BG118100 Rh4CG132200
rosa_wichuraiana Rw4G010060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 290, 332
AatII GACGTC 3 cut(s) 330, 393, 450
AccB1I GGYRCC 1 cut(s) 1327
AccB7I CCANNNNNTGG 3 cut(s) 261, 655, 2516
AccII CGCG 2 cut(s) 323, 693
AccIII TCCGGA 1 cut(s) 2530
AciI CCGC 7 cut(s) 138, 312, 323, 418, 691, 2501, 2522
AclI AACGTT 1 cut(s) 171
AclWI GGATC 7 cut(s) 64, 77, 631, 644, 1719, 1844, 2114
AcoI YGGCCR 2 cut(s) 573, 1204
AcsI RAATTY 4 cut(s) 31, 1321, 1448, 1528
AcuI CTGAAG 4 cut(s) 944, 1678, 1776, 2478
AcyI GRCGYC 3 cut(s) 327, 390, 447
AdeI CACNNNGTG 2 cut(s) 1962, 2218
AfaI GTAC 5 cut(s) 341, 550, 686, 887, 1202
AfiI CCNNNNNNNGG 8 cut(s) 66, 261, 622, 655, 1141, 1695, 2129, 2516
AflIII ACRYGT 1 cut(s) 567
AgsI TTSAA 7 cut(s) 787, 877, 1009, 1081, 1834, 2483, 2543
AjnI CCWGG 3 cut(s) 1496, 1958, 2139
AjuI GAANNNNNNNTTGG 4 cut(s) 569, 601, 1088, 1120
Alw21I GWGCWC 4 cut(s) 538, 1364, 2095, 2268
Alw26I GTCTC 5 cut(s) 214, 234, 1160, 1447, 1473
Alw44I GTGCAC 2 cut(s) 534, 2091
AlwI GGATC 7 cut(s) 64, 77, 631, 644, 1719, 1844, 2114
Ama87I CYCGRG 2 cut(s) 7, 1211
Aor13HI TCCGGA 1 cut(s) 2530
AoxI GGCC 9 cut(s) 57, 491, 561, 573, 582, 1204, 1241, 2206, 2485
ApaLI GTGCAC 2 cut(s) 534, 2091
ApeKI GCWGC 8 cut(s) 420, 511, 1099, 1265, 1400, 2029, 2152, 2498
ApoI RAATTY 4 cut(s) 31, 1321, 1448, 1528
ArsI GACNNNNNNTTYG 4 cut(s) 299, 331, 380, 412
Asp700I GAANNNNTTC 1 cut(s) 768
AspLEI GCGC 2 cut(s) 323, 1481
AspS9I GGNCC 7 cut(s) 37, 57, 292, 757, 1241, 1685, 2519
AsuC2I CCSGG 5 cut(s) 56, 617, 761, 1212, 1213
AsuHPI GGTGA 5 cut(s) 1403, 1948, 2153, 2289, 2302
AsuII TTCGAA 1 cut(s) 270
AvaI CYCGRG 2 cut(s) 7, 1211
AvaII GGWCC 5 cut(s) 37, 292, 757, 1685, 2519
AxyI CCTNAGG 1 cut(s) 1785
BaeGI GKGCMC 2 cut(s) 538, 2095
BaeI ACNNNNGTAYC 4 cut(s) 877, 910, 1199, 1232
BamHI GGATCC 2 cut(s) 69, 636
BanI GGYRCC 1 cut(s) 1327
BanII GRGCYC 4 cut(s) 781, 1364, 1621, 2268
BauI CACGAG 1 cut(s) 410
BbsI GAAGAC 4 cut(s) 252, 854, 1556, 1991
Bbv12I GWGCWC 4 cut(s) 538, 1364, 2095, 2268
BbvI GCAGC 8 cut(s) 407, 498, 1111, 1252, 1412, 2016, 2139, 2510
BccI CCATC 4 cut(s) 657, 1232, 1307, 2057
BceAI ACGGC 6 cut(s) 44, 325, 412, 484, 1219, 1786
BciT130I CCWGG 3 cut(s) 1498, 1960, 2141
BciVI GTATCC 1 cut(s) 2373
BclI TGATCA 2 cut(s) 1434, 2158
BcnI CCSGG 5 cut(s) 56, 617, 761, 1212, 1213
BcoDI GTCTC 5 cut(s) 214, 234, 1160, 1447, 1473
BfaI CTAG 2 cut(s) 1928, 2165
BfmI CTRYAG 1 cut(s) 844
BfuI GTATCC 1 cut(s) 2373
BglI GCCNNNNNGGC 1 cut(s) 581
BglII AGATCT 1 cut(s) 11
Bme1390I CCNGG 8 cut(s) 56, 617, 761, 1212, 1213, 1498, 1960, 2141
Bme18I GGWCC 5 cut(s) 37, 292, 757, 1685, 2519
BmeT110I CYCGRG 2 cut(s) 7, 1211
BmgT120I GGNCC 7 cut(s) 37, 57, 292, 757, 1241, 1685, 2519
BmiI GGNNCC 8 cut(s) 39, 71, 638, 806, 1329, 1686, 1687, 2043
BmrFI CCNGG 8 cut(s) 56, 617, 761, 1212, 1213, 1498, 1960, 2141
BmsI GCATC 8 cut(s) 1123, 1498, 1510, 1628, 1656, 2104, 2113, 2364
BoxI GACNNNNGTC 1 cut(s) 446
BpiI GAAGAC 4 cut(s) 252, 854, 1556, 1991
BplI GAGNNNNNCTC 2 cut(s) 2255, 2287
Bpu14I TTCGAA 1 cut(s) 270
BpuEI CTTGAG 1 cut(s) 694
BpuMI CCSGG 5 cut(s) 56, 617, 761, 1212, 1213
Bsa29I ATCGAT 1 cut(s) 1236
BsaAI YACGTR 3 cut(s) 230, 343, 568
BsaBI GATNNNNATC 1 cut(s) 2118
BsaHI GRCGYC 3 cut(s) 327, 390, 447
BsaI GGTCTC 1 cut(s) 1160
BsaJI CCNNGG 6 cut(s) 529, 1135, 1211, 1496, 1979, 2139
BsaWI WCCGGW 3 cut(s) 744, 754, 2530
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 8 cut(s) 66, 261, 622, 655, 1141, 1695, 2129, 2516
Bse118I RCCGGY 2 cut(s) 1746, 2188
Bse1I ACTGG 3 cut(s) 1170, 2100, 2238
Bse21I CCTNAGG 1 cut(s) 1785
Bse3DI GCAATG 3 cut(s) 1098, 1716, 2391
Bse8I GATNNNNATC 1 cut(s) 2118
BseAI TCCGGA 1 cut(s) 2530
BseBI CCWGG 3 cut(s) 1498, 1960, 2141
BseCI ATCGAT 1 cut(s) 1236
BseDI CCNNGG 6 cut(s) 529, 1135, 1211, 1496, 1979, 2139
BseJI GATNNNNATC 1 cut(s) 2118
BseLI CCNNNNNNNGG 8 cut(s) 66, 261, 622, 655, 1141, 1695, 2129, 2516
BseMI GCAATG 3 cut(s) 1098, 1716, 2391
BseMII CTCAG 4 cut(s) 764, 1799, 1912, 2346
BseNI ACTGG 3 cut(s) 1170, 2100, 2238
BseRI GAGGAG 3 cut(s) 395, 1648, 2276
BseSI GKGCMC 2 cut(s) 538, 2095
BseXI GCAGC 8 cut(s) 407, 498, 1111, 1252, 1412, 2016, 2139, 2510
BseYI CCCAGC 2 cut(s) 856, 1641
BsgI GTGCAG 1 cut(s) 1169
Bsh1236I CGCG 2 cut(s) 323, 693
Bsh1285I CGRYCG 2 cut(s) 130, 547
BshFI GGCC 9 cut(s) 59, 493, 563, 575, 584, 1206, 1243, 2208, 2487
BshNI GGYRCC 1 cut(s) 1327
BshVI ATCGAT 1 cut(s) 1236
BsiEI CGRYCG 2 cut(s) 130, 547
BsiHKAI GWGCWC 4 cut(s) 538, 1364, 2095, 2268
BsiHKCI CYCGRG 2 cut(s) 7, 1211
BslFI GGGAC 1 cut(s) 1698
BslI CCNNNNNNNGG 8 cut(s) 66, 261, 622, 655, 1141, 1695, 2129, 2516
BsmAI GTCTC 5 cut(s) 214, 234, 1160, 1447, 1473
BsmBI CGTCTC 3 cut(s) 214, 234, 1473
BsmFI GGGAC 1 cut(s) 1698
BsmI GAATGC 1 cut(s) 858
BsnI GGCC 9 cut(s) 59, 493, 563, 575, 584, 1206, 1243, 2208, 2487
Bso31I GGTCTC 1 cut(s) 1160
BsoBI CYCGRG 2 cut(s) 7, 1211
Bsp119I TTCGAA 1 cut(s) 270
Bsp1286I GDGCHC 6 cut(s) 538, 781, 1364, 1621, 2095, 2268
Bsp13I TCCGGA 1 cut(s) 2530
BspACI CCGC 7 cut(s) 138, 312, 323, 418, 691, 2501, 2522
BspANI GGCC 9 cut(s) 59, 493, 563, 575, 584, 1206, 1243, 2208, 2487
BspCNI CTCAG 4 cut(s) 765, 1798, 1911, 2345
BspDI ATCGAT 1 cut(s) 1236
BspEI TCCGGA 1 cut(s) 2530
BspFNI CGCG 2 cut(s) 323, 693
BspHI TCATGA 1 cut(s) 1600
BspLI GGNNCC 8 cut(s) 39, 71, 638, 806, 1329, 1686, 1687, 2043
BspPI GGATC 7 cut(s) 64, 77, 631, 644, 1719, 1844, 2114
BspQI GCTCTTC 1 cut(s) 1104
BspT104I TTCGAA 1 cut(s) 270
BspT107I GGYRCC 1 cut(s) 1327
BspTNI GGTCTC 1 cut(s) 1160
BsrDI GCAATG 3 cut(s) 1098, 1716, 2391
BsrFI RCCGGY 2 cut(s) 1746, 2188
BsrI ACTGG 3 cut(s) 1170, 2100, 2238
BssAI RCCGGY 2 cut(s) 1746, 2188
BssECI CCNNGG 6 cut(s) 529, 1135, 1211, 1496, 1979, 2139
BssNI GRCGYC 3 cut(s) 327, 390, 447
BssSI CACGAG 1 cut(s) 410
BssT1I CCWWGG 1 cut(s) 1979
Bst2BI CACGAG 1 cut(s) 410
Bst2UI CCWGG 3 cut(s) 1498, 1960, 2141
Bst4CI ACNGT 8 cut(s) 131, 184, 533, 548, 1345, 2090, 2416, 2461
Bst6I CTCTTC 4 cut(s) 759, 1104, 1548, 2073
BstACI GRCGYC 3 cut(s) 327, 390, 447
BstAPI GCANNNNNTGC 1 cut(s) 1274
BstBAI YACGTR 3 cut(s) 230, 343, 568
BstBI TTCGAA 1 cut(s) 270
BstC8I GCNNGC 8 cut(s) 87, 425, 526, 538, 561, 1748, 2388, 2410
BstDEI CTNAG 5 cut(s) 773, 1472, 1785, 1898, 2332
BstDSI CCRYGG 1 cut(s) 529
BstFNI CGCG 2 cut(s) 323, 693
BstHHI GCGC 2 cut(s) 323, 1481
BstMAI GTCTC 5 cut(s) 214, 234, 1160, 1447, 1473
BstMCI CGRYCG 2 cut(s) 130, 547
BstMWI GCNNNNNNNGC 5 cut(s) 318, 581, 1274, 1616, 2498
BstNI CCWGG 3 cut(s) 1498, 1960, 2141
BstNSI RCATGY 1 cut(s) 511
BstPAI GACNNNNGTC 1 cut(s) 446
BstSCI CCNGG 8 cut(s) 54, 615, 759, 1210, 1211, 1496, 1958, 2139
BstSFI CTRYAG 1 cut(s) 844
BstSLI GKGCMC 2 cut(s) 538, 2095
BstUI CGCG 2 cut(s) 323, 693
BstV1I GCAGC 8 cut(s) 407, 498, 1111, 1252, 1412, 2016, 2139, 2510
BstV2I GAAGAC 4 cut(s) 252, 854, 1556, 1991
BstX2I RGATCY 3 cut(s) 11, 69, 636
BstXI CCANNNNNNTGG 1 cut(s) 1531
BstYI RGATCY 3 cut(s) 11, 69, 636
Bsu15I ATCGAT 1 cut(s) 1236
Bsu36I CCTNAGG 1 cut(s) 1785
BsuI GTATCC 1 cut(s) 2373
BsuRI GGCC 9 cut(s) 59, 493, 563, 575, 584, 1206, 1243, 2208, 2487
BsuTUI ATCGAT 1 cut(s) 1236
BtgI CCRYGG 1 cut(s) 529
BtsI GCAGTG 3 cut(s) 1819, 1852, 1922
Cac8I GCNNGC 8 cut(s) 87, 425, 526, 538, 561, 1748, 2388, 2410
CciI TCATGA 1 cut(s) 1600
CfoI GCGC 2 cut(s) 323, 1481
Cfr10I RCCGGY 2 cut(s) 1746, 2188
Cfr13I GGNCC 7 cut(s) 37, 57, 292, 757, 1241, 1685, 2519
Cfr9I CCCGGG 1 cut(s) 1211
ClaI ATCGAT 1 cut(s) 1236
CpoI CGGWCCG 1 cut(s) 757
CseI GACGC 3 cut(s) 682, 1261, 1550
CsiI ACCWGGT 1 cut(s) 1958
Csp6I GTAC 5 cut(s) 340, 549, 685, 886, 1201
CspI CGGWCCG 1 cut(s) 757
CviQI GTAC 5 cut(s) 340, 549, 685, 886, 1201
DdeI CTNAG 5 cut(s) 773, 1472, 1785, 1898, 2332
DraIII CACNNNGTG 2 cut(s) 1962, 2218
DrdI GACNNNNNNGTC 2 cut(s) 290, 332
DseDI GACNNNNNNGTC 2 cut(s) 290, 332
EaeI YGGCCR 2 cut(s) 573, 1204
Eam1104I CTCTTC 4 cut(s) 759, 1104, 1548, 2073
EarI CTCTTC 4 cut(s) 759, 1104, 1548, 2073
Ecl136II GAGCTC 2 cut(s) 1362, 2266
Eco130I CCWWGG 1 cut(s) 1979
Eco147I AGGCCT 3 cut(s) 563, 584, 2487
Eco24I GRGCYC 4 cut(s) 781, 1364, 1621, 2268
Eco31I GGTCTC 1 cut(s) 1160
Eco47I GGWCC 5 cut(s) 37, 292, 757, 1685, 2519
Eco53kI GAGCTC 2 cut(s) 1362, 2266
Eco57I CTGAAG 4 cut(s) 944, 1678, 1776, 2478
Eco81I CCTNAGG 1 cut(s) 1785
Eco88I CYCGRG 2 cut(s) 7, 1211
EcoICRI GAGCTC 2 cut(s) 1362, 2266
EcoO109I RGGNCCY 1 cut(s) 1685
EcoRII CCWGG 3 cut(s) 1496, 1958, 2139
EcoT14I CCWWGG 1 cut(s) 1979
EcoT22I ATGCAT 1 cut(s) 2318
EcoT38I GRGCYC 4 cut(s) 781, 1364, 1621, 2268
ErhI CCWWGG 1 cut(s) 1979
Esp3I CGTCTC 3 cut(s) 214, 234, 1473
FalI AAGNNNNNCTT 2 cut(s) 2139, 2171
FaqI GGGAC 1 cut(s) 1698
FauNDI CATATG 1 cut(s) 948
FbaI TGATCA 2 cut(s) 1434, 2158
FriOI GRGCYC 4 cut(s) 781, 1364, 1621, 2268
FspBI CTAG 2 cut(s) 1928, 2165
GlaI GCGC 2 cut(s) 322, 1480
GsaI CCCAGC 2 cut(s) 860, 1645
HaeIII GGCC 9 cut(s) 59, 493, 563, 575, 584, 1206, 1243, 2208, 2487
HgaI GACGC 3 cut(s) 682, 1261, 1550
HhaI GCGC 2 cut(s) 323, 1481
Hin1I GRCGYC 3 cut(s) 327, 390, 447
Hin6I GCGC 2 cut(s) 321, 1479
HinP1I GCGC 2 cut(s) 321, 1479
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HindIII AAGCTT 2 cut(s) 698, 1700
HphI GGTGA 5 cut(s) 1403, 1948, 2153, 2289, 2302
Hpy166II GTNNAC 7 cut(s) 454, 536, 551, 1798, 1965, 2093, 2242
Hpy8I GTNNAC 7 cut(s) 454, 536, 551, 1798, 1965, 2093, 2242
Hpy99I CGWCG 9 cut(s) 128, 332, 392, 395, 445, 452, 733, 736, 1255
HpyAV CCTTC 4 cut(s) 595, 994, 1003, 1882
HpyCH4III ACNGT 8 cut(s) 131, 184, 533, 548, 1345, 2090, 2416, 2461
HpyCH4IV ACGT 8 cut(s) 171, 229, 327, 342, 390, 447, 567, 630
HpyF10VI GCNNNNNNNGC 5 cut(s) 318, 581, 1274, 1616, 2498
HpyF3I CTNAG 5 cut(s) 773, 1472, 1785, 1898, 2332
HpySE526I ACGT 8 cut(s) 171, 229, 327, 342, 390, 447, 567, 630
Hsp92I GRCGYC 3 cut(s) 327, 390, 447
HspAI GCGC 2 cut(s) 321, 1479
KflI GGGWCCC 1 cut(s) 1685
Kpn2I TCCGGA 1 cut(s) 2530
KroI GCCGGC 1 cut(s) 1746
KroNI GCCGGC 1 cut(s) 1748
Ksp22I TGATCA 2 cut(s) 1434, 2158
LguI GCTCTTC 1 cut(s) 1104
LmnI GCTCC 5 cut(s) 1359, 1810, 1997, 2041, 2263
Lsp1109I GCAGC 8 cut(s) 407, 498, 1111, 1252, 1412, 2016, 2139, 2510
LweI GCATC 8 cut(s) 1123, 1498, 1510, 1628, 1656, 2104, 2113, 2364
MabI ACCWGGT 1 cut(s) 1958
MaeI CTAG 2 cut(s) 1928, 2165
MaeII ACGT 8 cut(s) 171, 229, 327, 342, 390, 447, 567, 630
MaeIII GTNAC 3 cut(s) 1246, 1949, 2295
MflI RGATCY 3 cut(s) 11, 69, 636
MhlI GDGCHC 6 cut(s) 538, 781, 1364, 1621, 2095, 2268
MluCI AATT 6 cut(s) 31, 787, 1321, 1448, 1528, 1834
MlyI GAGTC 2 cut(s) 87, 369
MmeI TCCRAC 9 cut(s) 47, 147, 183, 237, 322, 714, 1362, 1527, 1994
Mph1103I ATGCAT 1 cut(s) 2318
MroI TCCGGA 1 cut(s) 2530
MroNI GCCGGC 1 cut(s) 1746
MroXI GAANNNNTTC 1 cut(s) 768
MseI TTAA 6 cut(s) 353, 924, 1065, 1299, 2199, 2349
MslI CAYNNNNRTG 2 cut(s) 1541, 2112
MspA1I CMGCKG 2 cut(s) 420, 2501
MspR9I CCNGG 8 cut(s) 56, 617, 761, 1212, 1213, 1498, 1960, 2141
Mva1269I GAATGC 1 cut(s) 858
MvaI CCWGG 3 cut(s) 1498, 1960, 2141
MvnI CGCG 2 cut(s) 323, 693
MwoI GCNNNNNNNGC 5 cut(s) 318, 581, 1274, 1616, 2498
NaeI GCCGGC 1 cut(s) 1748
NciI CCSGG 5 cut(s) 56, 617, 761, 1212, 1213
NdeI CATATG 1 cut(s) 948
NgoMIV GCCGGC 1 cut(s) 1746
NlaIV GGNNCC 8 cut(s) 39, 71, 638, 806, 1329, 1686, 1687, 2043
NmeAIII GCCGAG 1 cut(s) 1160
NmuCI GTSAC 2 cut(s) 1246, 2295
NsiI ATGCAT 1 cut(s) 2318
NspI RCATGY 1 cut(s) 511
NspV TTCGAA 1 cut(s) 270
PaeR7I CTCGAG 1 cut(s) 7
PagI TCATGA 1 cut(s) 1600
PceI AGGCCT 3 cut(s) 563, 584, 2487
PciSI GCTCTTC 1 cut(s) 1104
PcsI WCGNNNNNNNCGW 3 cut(s) 235, 375, 544
PctI GAATGC 1 cut(s) 858
PdiI GCCGGC 1 cut(s) 1748
PdmI GAANNNNTTC 1 cut(s) 768
PfeI GAWTC 9 cut(s) 201, 597, 751, 769, 943, 1665, 1892, 1973, 2369
PflFI GACNNNGTC 2 cut(s) 389, 449
PflMI CCANNNNNTGG 3 cut(s) 261, 655, 2516
PfoI TCCNGGA 1 cut(s) 759
PleI GAGTC 2 cut(s) 86, 368
PpsI GAGTC 2 cut(s) 86, 368
Ppu21I YACGTR 3 cut(s) 230, 343, 568
PpuMI RGGWCCY 1 cut(s) 1685
PshAI GACNNNNGTC 1 cut(s) 446
Psp124BI GAGCTC 2 cut(s) 1364, 2268
Psp1406I AACGTT 1 cut(s) 171
Psp5II RGGWCCY 1 cut(s) 1685
Psp6I CCWGG 3 cut(s) 1496, 1958, 2139
PspFI CCCAGC 2 cut(s) 856, 1641
PspGI CCWGG 3 cut(s) 1496, 1958, 2139
PspN4I GGNNCC 8 cut(s) 39, 71, 638, 806, 1329, 1686, 1687, 2043
PspPI GGNCC 7 cut(s) 37, 57, 292, 757, 1241, 1685, 2519
PspPPI RGGWCCY 1 cut(s) 1685
PsuI RGATCY 3 cut(s) 11, 69, 636
PsyI GACNNNGTC 2 cut(s) 389, 449
RsaI GTAC 5 cut(s) 341, 550, 686, 887, 1202
RsaNI GTAC 5 cut(s) 340, 549, 685, 886, 1201
RseI CAYNNNNRTG 2 cut(s) 1541, 2112
Rsr2I CGGWCCG 1 cut(s) 757
RsrII CGGWCCG 1 cut(s) 757
SacI GAGCTC 2 cut(s) 1364, 2268
SapI GCTCTTC 1 cut(s) 1104
SaqAI TTAA 6 cut(s) 353, 924, 1065, 1299, 2199, 2349
Sau96I GGNCC 7 cut(s) 37, 57, 292, 757, 1241, 1685, 2519
SchI GAGTC 2 cut(s) 87, 369
ScrFI CCNGG 8 cut(s) 56, 617, 761, 1212, 1213, 1498, 1960, 2141
SduI GDGCHC 6 cut(s) 538, 781, 1364, 1621, 2095, 2268
SexAI ACCWGGT 1 cut(s) 1958
SfaNI GCATC 8 cut(s) 1123, 1498, 1510, 1628, 1656, 2104, 2113, 2364
SfcI CTRYAG 1 cut(s) 844
SfiI GGCCNNNNNGGCC 1 cut(s) 581
Sfr274I CTCGAG 1 cut(s) 7
SfuI TTCGAA 1 cut(s) 270
SinI GGWCC 5 cut(s) 37, 292, 757, 1685, 2519
SlaI CTCGAG 1 cut(s) 7
SmaI CCCGGG 1 cut(s) 1213
SmiMI CAYNNNNRTG 2 cut(s) 1541, 2112
SmlI CTYRAG 2 cut(s) 7, 673
SmoI CTYRAG 2 cut(s) 7, 673
Sse9I AATT 6 cut(s) 31, 787, 1321, 1448, 1528, 1834
SseBI AGGCCT 3 cut(s) 563, 584, 2487
SsiI CCGC 7 cut(s) 138, 312, 323, 418, 691, 2501, 2522
SspMI CTAG 2 cut(s) 1928, 2165
SstI GAGCTC 2 cut(s) 1364, 2268
StuI AGGCCT 3 cut(s) 563, 584, 2487
StyD4I CCNGG 8 cut(s) 54, 615, 759, 1210, 1211, 1496, 1958, 2139
StyI CCWWGG 1 cut(s) 1979
TaaI ACNGT 8 cut(s) 131, 184, 533, 548, 1345, 2090, 2416, 2461
TaiI ACGT 8 cut(s) 174, 232, 330, 345, 393, 450, 570, 633
TaqII GACCGA 1 cut(s) 1816
TasI AATT 6 cut(s) 31, 787, 1321, 1448, 1528, 1834
TauI GCSGC 3 cut(s) 141, 315, 2504
TfiI GAWTC 9 cut(s) 201, 597, 751, 769, 943, 1665, 1892, 1973, 2369
Tru1I TTAA 6 cut(s) 353, 924, 1065, 1299, 2199, 2349
Tru9I TTAA 6 cut(s) 353, 924, 1065, 1299, 2199, 2349
TseFI GTSAC 2 cut(s) 1246, 2295
TseI GCWGC 8 cut(s) 420, 511, 1099, 1265, 1400, 2029, 2152, 2498
Tsp45I GTSAC 2 cut(s) 1246, 2295
TspDTI ATGAA 8 cut(s) 88, 854, 935, 1109, 1334, 1541, 1858, 2539
TspGWI ACGGA 6 cut(s) 372, 458, 717, 1455, 1475, 2367
TspMI CCCGGG 1 cut(s) 1211
Tth111I GACNNNGTC 2 cut(s) 389, 449
Van91I CCANNNNNTGG 3 cut(s) 261, 655, 2516
VneI GTGCAC 2 cut(s) 534, 2091
VpaK11BI GGWCC 5 cut(s) 37, 292, 757, 1685, 2519
XapI RAATTY 4 cut(s) 31, 1321, 1448, 1528
XceI RCATGY 1 cut(s) 511
XhoI CTCGAG 1 cut(s) 7
XmaI CCCGGG 1 cut(s) 1211
XmnI GAANNNNTTC 1 cut(s) 768
XspI CTAG 2 cut(s) 1928, 2165
ZraI GACGTC 3 cut(s) 328, 391, 448
Zsp2I ATGCAT 1 cut(s) 2318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.