Rroxscaffold_5G00348790

Glycosyltransferase family 20

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0017477
Physical Location & Seq
Reverse (-)
21272108 .. 21275380
3273 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00348790.1

Sequence Viewer

Length: 2634 bp
ATGCTCTCGAGATCTTGCTTTGATTTACTGAATTTGGACCCCATTGACGATTTCCGGGCCGTCAGTCGGATCCCCAGAGTCATGCAAGCCCCCGGCTTCATCTCCGAGTTAGACAGCAACTCCGACGACCGTTCCGGCGGCTCATCCTCATCCGACAACAACAACAACAACAACGTTGTTTCCTCTGTTTCGCAGGAACGAAGAATCATCGTCTCCAACCACTTGCCAATACGCGCGTTTCGAGACGATAACGAGACCAACAAGTGGCGTTTCGAATACGACCACGACAGCTTGGTCCTCCAACTCAAAGACGGCGGCGGCTTTGGCGCGGACGTCGAGGTCTTGTACGTGGGTTGCTTAAACGGAGCCGAGATCGACCCCTGCGATCAAGACGACGTCGCATTTGTCCTCCTCCACGAGTTCCACTGCGTGCCGTGTTTTTTACCGACGGAAGTCGTCAACAAGTTTTATCACGGCTTCTGTAAGCACTATCTTTGGCCTCTGTTTCACTACATGCTGCCCATGTCAGCCAGCCACGGTGCACGCTTTGACCGTGCACTCTGGCAGGCCTACGTGTCGGCCAACAAGGCCTTCGCCGATAGAATCATAGAGGTTCTAAACCCGGATGAGGACTACGTTTGGATCCATGATTACCATCTCATGGTGTTGCCTACTTTCTTGAGGAAAAGGTACTACCGCGTCAAGCTTGGTTTCTTTCTGCATAGTCCTTTTCCGTCGTCGGAGATTTACCGGACGATTCCCGTCCGGGAGGAGATTCTGAGGGCTCTGTTGAATTGTGATCTTATTGGGTTCCATATATTTGATTATGCGAGGCACTTCTTGTCTTGCTGTAGCAGAATGCTGGGTCTTCATTATGAGTTCAAAAGAGGGTACATGGGTTTGGAGTATTATGGTCGAATAGTGAGTATTAAGATGTTGCCTGTGGGGATTCATATGGGTCAGCTTCAGTCTGTTTTGTCGCTTGATGATACTGCCAAGAAAGTGAAGGTGTTGAAGGACAAGTTTGAGGGGAAGATTGTGATTTTGGGGGTGGATGATTTGGACTTGTTTAAGGGGATTAGTTTGAAGTTTCTGGCAATGAAGCAGCTTTTGGAAGAGCATCAGAGTTTGCGGGGGAAGGTGGTTATGGTGCAGATAACAAACCCGGCACGAAGCCGAGGCAAGGATGTGCAGGAGGTTCTGAATGAGACCAGTGCTATTGCAAAAGAGATCAATGACAAGTATGGCCAACCCGGGTATCAGCCCCTTGTTGTCATCGATGGACCTGTGACGACGCAAGACAAAGCTGCATATTATGCCATTTCGGAGTGCTGTTTGGTTAATGCTGTGAGGGATGGGATGAATTTGGTGCCTTACACGTACACTGTTTGTAGGCAGGGGAGCTCTGTTTTGGACAAGGTATTGGAGATTGGTGATGAGGCAGCAGATACGACACCTAAACCGAAAAAGAGTGTGATCATTGTCTCTGAATTTATTGGCTGTTCTCCGTCTCTTAGTGGAGCAATCCGTGTCAATCCCTGGAACATTGATGCTGTCTCTGATGCCATGAATTTGGTCATCACAATGTCGGAGGAAGAGAAGCGTCTTCGCCATGACAAACATTACAAGTATATCAGCTCTCATGATGTTGCTTATTGGGCTCGGAGTTTTGATCAAGATCTTGAGAGAGCTTGCAGAGAGCATTATCGGAGGAGATGCTGGGGCATCGGTTTAGGTTTGGGATTCAGAGTTGTAGCTTTGGGACCCAACTTCCGGAAGCTTTCTGTGGATCACATTGCCTATGCTTACAGAAATACAAGTAGCCGGCTCATCTTATTGGACTATGACGGCACTATGACACCTCAGGCTTCAGTGGACAAAGCTCCAAGCAGTGAAGTTATTTCAGTCTTGAATTGCTTATGCAGTGATCCGAAAAACATTGTTTTCATTGTGAGTGGCAGAGGGAAGGATTCTCTCAGCAAGTGGTTTTCGCAGTGTGAGAAACTAGGTTTATCAGCAGAGCATGGTTACTTCACCAGGTGGTCTAAAGATTCTCCTTGGAAGACTTGCTCCCTGACAATGGATTTCAGTTGGAAGAAGATTGTGCTGCCTATTATGGAGCCATACACAGCAGCAACCGATGGTTCTTTTATAGAAGAGAAAGACAGTGCACTGGTCTGGCATCATCAAGATGCTGATCCTCATTTCGGCTCATCCCAGGCTAAAGAGCTGCTTGATCACCTAGAGAGTGTGCTTGTCAATGAGCCGGTTGTAGTTAAAAGAGGCCAACACATTGTGGAAGTGAAACCACTGGATGTGAACAAAGGTATAGTTGTGGAGGAGCTCATCTCCACTATGCGAAATAGGGTGAAGTCACCAGATTTCTTGTTATGCATAGGGGATGATCGCTCAGATGAGGATATGTTTAAGAGCATCGTGTATCCCGAATCAAGTCCGTCCTTGCCAGCCATTGCAGAAGTCTTTGCTTGCACTGTTGGTCAAAAACCAAGCATGGCAAAATACTATCTGGATGATACAGTTGATGTCATCAAGTTGCTTCAAGGCCTCGCTTCAGCGTCAGCGCAACCAAAGTCTGGACCGCAGTTTCCGGAAGCATTTGAAGTGTTCATTTGA

Protein Analysis

877

Amino Acids

99.11

Weight (kDa)

5.96

Isoelectric Point (pI)

48.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_20 PF00982 67 - 562 1.6e-175 Glycosyltransferase family 20
Trehalose_PPase PF02358 612 - 846 9.8e-80 Trehalose-phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18700
fragaria_vesca FvH4_4g09780
prunus_persica Prupe.1G095500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0404641
rosa_laevigata RLG00000008917
rosa_multiflora Rmu_sc0002699.1_g000030
rosa_roxburghii Rroxscaffold_5G00348790
rosa_rugosa Rorug04G0048300
rosa_samantha Rh4AG124100 Rh4BG118100 Rh4CG132200
rosa_wichuraiana Rw4G010060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 293, 338
AatII GACGTC 2 cut(s) 336, 399
AccB1I GGYRCC 1 cut(s) 1369
AccB7I CCANNNNNTGG 3 cut(s) 264, 661, 2594
AccII CGCG 4 cut(s) 234, 236, 329, 699
AccIII TCCGGA 2 cut(s) 1773, 2608
AciI CCGC 7 cut(s) 138, 315, 318, 329, 697, 1132, 2600
AclI AACGTT 1 cut(s) 174
AclWI GGATC 7 cut(s) 64, 77, 637, 650, 1797, 1922, 2192
AcoI YGGCCR 2 cut(s) 579, 1246
AcsI RAATTY 4 cut(s) 31, 1363, 1490, 1570
AcuI CTGAAG 3 cut(s) 950, 1854, 2556
AcyI GRCGYC 2 cut(s) 333, 396
AdeI CACNNNGTG 3 cut(s) 430, 2040, 2296
AfaI GTAC 4 cut(s) 347, 692, 893, 1382
AfiI CCNNNNNNNGG 9 cut(s) 66, 264, 628, 661, 1183, 1773, 2080, 2207, 2594
AflIII ACRYGT 2 cut(s) 573, 1377
AgsI TTSAA 7 cut(s) 793, 883, 1015, 1087, 1912, 2561, 2621
AjnI CCWGG 3 cut(s) 1538, 2036, 2217
AjuI GAANNNNNNNTTGG 4 cut(s) 575, 607, 1094, 1126
Alw21I GWGCWC 5 cut(s) 544, 559, 1406, 2173, 2346
Alw26I GTCTC 7 cut(s) 217, 237, 248, 1202, 1489, 1515, 1561
Alw44I GTGCAC 3 cut(s) 540, 555, 2169
AlwI GGATC 7 cut(s) 64, 77, 637, 650, 1797, 1922, 2192
Ama87I CYCGRG 2 cut(s) 7, 1253
Aor13HI TCCGGA 2 cut(s) 1773, 2608
AoxI GGCC 8 cut(s) 57, 497, 567, 579, 588, 1246, 2284, 2563
ApaLI GTGCAC 3 cut(s) 540, 555, 2169
ApeKI GCWGC 7 cut(s) 517, 1105, 1307, 1442, 2107, 2132, 2230
ApoI RAATTY 4 cut(s) 31, 1363, 1490, 1570
ArsI GACNNNNNNTTYG 2 cut(s) 386, 418
AspLEI GCGC 3 cut(s) 236, 329, 2584
AspS9I GGNCC 6 cut(s) 37, 57, 295, 1283, 1763, 2597
AsuC2I CCSGG 7 cut(s) 56, 93, 623, 767, 1166, 1254, 1255
AsuHPI GGTGA 5 cut(s) 1445, 2026, 2231, 2367, 2380
AsuII TTCGAA 1 cut(s) 273
AvaI CYCGRG 2 cut(s) 7, 1253
AvaII GGWCC 5 cut(s) 37, 295, 1283, 1763, 2597
AxyI CCTNAGG 1 cut(s) 1863
BaeGI GKGCMC 3 cut(s) 544, 559, 2173
BaeI ACNNNNGTAYC 4 cut(s) 883, 916, 1241, 1274
BalI TGGCCA 1 cut(s) 1248
BamHI GGATCC 2 cut(s) 69, 642
BanI GGYRCC 1 cut(s) 1369
BanII GRGCYC 4 cut(s) 787, 1406, 1663, 2346
BauI CACGAG 1 cut(s) 416
BbsI GAAGAC 3 cut(s) 860, 1598, 2069
Bbv12I GWGCWC 5 cut(s) 544, 559, 1406, 2173, 2346
BbvI GCAGC 7 cut(s) 504, 1117, 1294, 1454, 2094, 2144, 2217
BccI CCATC 4 cut(s) 663, 1274, 1349, 2135
BceAI ACGGC 5 cut(s) 44, 328, 418, 490, 1864
BciT130I CCWGG 3 cut(s) 1540, 2038, 2219
BciVI GTATCC 1 cut(s) 2451
BclI TGATCA 3 cut(s) 1476, 1672, 2236
BcnI CCSGG 7 cut(s) 56, 93, 623, 767, 1166, 1254, 1255
BcoDI GTCTC 7 cut(s) 217, 237, 248, 1202, 1489, 1515, 1561
BfaI CTAG 2 cut(s) 2006, 2243
BfmI CTRYAG 1 cut(s) 850
BfuI GTATCC 1 cut(s) 2451
BglI GCCNNNNNGGC 1 cut(s) 587
BglII AGATCT 2 cut(s) 11, 1678
Bme18I GGWCC 5 cut(s) 37, 295, 1283, 1763, 2597
BmeT110I CYCGRG 2 cut(s) 7, 1253
BmgT120I GGNCC 6 cut(s) 37, 57, 295, 1283, 1763, 2597
BmiI GGNNCC 9 cut(s) 39, 71, 367, 644, 812, 1371, 1764, 1765, 2121
BmsI GCATC 8 cut(s) 1129, 1540, 1552, 1706, 1734, 2182, 2191, 2442
BoxI GACNNNNGTC 1 cut(s) 452
BpiI GAAGAC 3 cut(s) 860, 1598, 2069
BplI GAGNNNNNCTC 2 cut(s) 2333, 2365
Bpu14I TTCGAA 1 cut(s) 273
BpuEI CTTGAG 2 cut(s) 700, 1703
BpuMI CCSGG 7 cut(s) 56, 93, 623, 767, 1166, 1254, 1255
Bsa29I ATCGAT 1 cut(s) 1278
BsaAI YACGTR 3 cut(s) 349, 574, 1380
BsaBI GATNNNNATC 3 cut(s) 654, 1677, 2196
BsaHI GRCGYC 2 cut(s) 333, 396
BsaI GGTCTC 2 cut(s) 248, 1202
BsaJI CCNNGG 7 cut(s) 91, 535, 1177, 1253, 1538, 2057, 2217
BsaWI WCCGGW 3 cut(s) 750, 1773, 2608
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 9 cut(s) 66, 264, 628, 661, 1183, 1773, 2080, 2207, 2594
Bse118I RCCGGY 2 cut(s) 1824, 2266
Bse1I ACTGG 3 cut(s) 1212, 2178, 2316
Bse21I CCTNAGG 1 cut(s) 1863
Bse3DI GCAATG 3 cut(s) 1104, 1794, 2469
Bse8I GATNNNNATC 3 cut(s) 654, 1677, 2196
BseAI TCCGGA 2 cut(s) 1773, 2608
BseBI CCWGG 3 cut(s) 1540, 2038, 2219
BseCI ATCGAT 1 cut(s) 1278
BseDI CCNNGG 7 cut(s) 91, 535, 1177, 1253, 1538, 2057, 2217
BseJI GATNNNNATC 3 cut(s) 654, 1677, 2196
BseLI CCNNNNNNNGG 9 cut(s) 66, 264, 628, 661, 1183, 1773, 2080, 2207, 2594
BseMI GCAATG 3 cut(s) 1104, 1794, 2469
BseMII CTCAG 4 cut(s) 770, 1877, 1990, 2424
BseNI ACTGG 3 cut(s) 1212, 2178, 2316
BseRI GAGGAG 4 cut(s) 401, 785, 1726, 2354
BseSI GKGCMC 3 cut(s) 544, 559, 2173
BseXI GCAGC 7 cut(s) 504, 1117, 1294, 1454, 2094, 2144, 2217
BseYI CCCAGC 2 cut(s) 862, 1719
BsgI GTGCAG 2 cut(s) 1172, 1211
Bsh1236I CGCG 4 cut(s) 234, 236, 329, 699
Bsh1285I CGRYCG 1 cut(s) 130
BshFI GGCC 8 cut(s) 59, 499, 569, 581, 590, 1248, 2286, 2565
BshNI GGYRCC 1 cut(s) 1369
BshVI ATCGAT 1 cut(s) 1278
BsiEI CGRYCG 1 cut(s) 130
BsiHKAI GWGCWC 5 cut(s) 544, 559, 1406, 2173, 2346
BsiHKCI CYCGRG 2 cut(s) 7, 1253
BslFI GGGAC 1 cut(s) 1776
BslI CCNNNNNNNGG 9 cut(s) 66, 264, 628, 661, 1183, 1773, 2080, 2207, 2594
BsmAI GTCTC 7 cut(s) 217, 237, 248, 1202, 1489, 1515, 1561
BsmBI CGTCTC 3 cut(s) 217, 237, 1515
BsmFI GGGAC 1 cut(s) 1776
BsmI GAATGC 1 cut(s) 864
BsnI GGCC 8 cut(s) 59, 499, 569, 581, 590, 1248, 2286, 2565
Bso31I GGTCTC 2 cut(s) 248, 1202
BsoBI CYCGRG 2 cut(s) 7, 1253
Bsp119I TTCGAA 1 cut(s) 273
Bsp1286I GDGCHC 7 cut(s) 544, 559, 787, 1406, 1663, 2173, 2346
Bsp13I TCCGGA 2 cut(s) 1773, 2608
BspACI CCGC 7 cut(s) 138, 315, 318, 329, 697, 1132, 2600
BspANI GGCC 8 cut(s) 59, 499, 569, 581, 590, 1248, 2286, 2565
BspCNI CTCAG 4 cut(s) 771, 1876, 1989, 2423
BspDI ATCGAT 1 cut(s) 1278
BspEI TCCGGA 2 cut(s) 1773, 2608
BspFNI CGCG 4 cut(s) 234, 236, 329, 699
BspHI TCATGA 1 cut(s) 1642
BspLI GGNNCC 9 cut(s) 39, 71, 367, 644, 812, 1371, 1764, 1765, 2121
BspPI GGATC 7 cut(s) 64, 77, 637, 650, 1797, 1922, 2192
BspQI GCTCTTC 1 cut(s) 1110
BspT104I TTCGAA 1 cut(s) 273
BspT107I GGYRCC 1 cut(s) 1369
BspTNI GGTCTC 2 cut(s) 248, 1202
BsrDI GCAATG 3 cut(s) 1104, 1794, 2469
BsrFI RCCGGY 2 cut(s) 1824, 2266
BsrI ACTGG 3 cut(s) 1212, 2178, 2316
BssAI RCCGGY 2 cut(s) 1824, 2266
BssECI CCNNGG 7 cut(s) 91, 535, 1177, 1253, 1538, 2057, 2217
BssNI GRCGYC 2 cut(s) 333, 396
BssSI CACGAG 1 cut(s) 416
BssT1I CCWWGG 1 cut(s) 2057
Bst2BI CACGAG 1 cut(s) 416
Bst2UI CCWGG 3 cut(s) 1540, 2038, 2219
Bst4CI ACNGT 7 cut(s) 131, 539, 554, 1387, 2168, 2494, 2539
Bst6I CTCTTC 3 cut(s) 1110, 1590, 2151
BstACI GRCGYC 2 cut(s) 333, 396
BstAPI GCANNNNNTGC 1 cut(s) 1316
BstBAI YACGTR 3 cut(s) 349, 574, 1380
BstBI TTCGAA 1 cut(s) 273
BstC8I GCNNGC 9 cut(s) 87, 431, 532, 544, 567, 1693, 1826, 2466, 2488
BstDEI CTNAG 5 cut(s) 779, 1514, 1863, 1976, 2410
BstDSI CCRYGG 1 cut(s) 535
BstFNI CGCG 4 cut(s) 234, 236, 329, 699
BstHHI GCGC 3 cut(s) 236, 329, 2584
BstMAI GTCTC 7 cut(s) 217, 237, 248, 1202, 1489, 1515, 1561
BstMCI CGRYCG 1 cut(s) 130
BstMWI GCNNNNNNNGC 4 cut(s) 324, 587, 1316, 1658
BstNI CCWGG 3 cut(s) 1540, 2038, 2219
BstNSI RCATGY 1 cut(s) 517
BstPAI GACNNNNGTC 1 cut(s) 452
BstSFI CTRYAG 1 cut(s) 850
BstSLI GKGCMC 3 cut(s) 544, 559, 2173
BstUI CGCG 4 cut(s) 234, 236, 329, 699
BstV1I GCAGC 7 cut(s) 504, 1117, 1294, 1454, 2094, 2144, 2217
BstV2I GAAGAC 3 cut(s) 860, 1598, 2069
BstX2I RGATCY 4 cut(s) 11, 69, 642, 1678
BstXI CCANNNNNNTGG 1 cut(s) 1573
BstYI RGATCY 4 cut(s) 11, 69, 642, 1678
Bsu15I ATCGAT 1 cut(s) 1278
Bsu36I CCTNAGG 1 cut(s) 1863
BsuI GTATCC 1 cut(s) 2451
BsuRI GGCC 8 cut(s) 59, 499, 569, 581, 590, 1248, 2286, 2565
BsuTUI ATCGAT 1 cut(s) 1278
BtgI CCRYGG 1 cut(s) 535
BtsI GCAGTG 4 cut(s) 424, 1897, 1930, 2000
Cac8I GCNNGC 9 cut(s) 87, 431, 532, 544, 567, 1693, 1826, 2466, 2488
CciI TCATGA 1 cut(s) 1642
CfoI GCGC 3 cut(s) 236, 329, 2584
Cfr10I RCCGGY 2 cut(s) 1824, 2266
Cfr13I GGNCC 6 cut(s) 37, 57, 295, 1283, 1763, 2597
Cfr9I CCCGGG 1 cut(s) 1253
ClaI ATCGAT 1 cut(s) 1278
CseI GACGC 4 cut(s) 688, 1303, 1592, 2565
CsiI ACCWGGT 1 cut(s) 2036
Csp6I GTAC 4 cut(s) 346, 691, 892, 1381
CviQI GTAC 4 cut(s) 346, 691, 892, 1381
DdeI CTNAG 5 cut(s) 779, 1514, 1863, 1976, 2410
DraIII CACNNNGTG 3 cut(s) 430, 2040, 2296
DrdI GACNNNNNNGTC 2 cut(s) 293, 338
DseDI GACNNNNNNGTC 2 cut(s) 293, 338
EaeI YGGCCR 2 cut(s) 579, 1246
Eam1104I CTCTTC 3 cut(s) 1110, 1590, 2151
EarI CTCTTC 3 cut(s) 1110, 1590, 2151
Ecl136II GAGCTC 2 cut(s) 1404, 2344
Eco130I CCWWGG 1 cut(s) 2057
Eco147I AGGCCT 3 cut(s) 569, 590, 2565
Eco24I GRGCYC 4 cut(s) 787, 1406, 1663, 2346
Eco31I GGTCTC 2 cut(s) 248, 1202
Eco47I GGWCC 5 cut(s) 37, 295, 1283, 1763, 2597
Eco53kI GAGCTC 2 cut(s) 1404, 2344
Eco57I CTGAAG 3 cut(s) 950, 1854, 2556
Eco81I CCTNAGG 1 cut(s) 1863
Eco88I CYCGRG 2 cut(s) 7, 1253
EcoICRI GAGCTC 2 cut(s) 1404, 2344
EcoO109I RGGNCCY 1 cut(s) 1763
EcoRII CCWGG 3 cut(s) 1538, 2036, 2217
EcoT14I CCWWGG 1 cut(s) 2057
EcoT22I ATGCAT 1 cut(s) 2396
EcoT38I GRGCYC 4 cut(s) 787, 1406, 1663, 2346
ErhI CCWWGG 1 cut(s) 2057
Esp3I CGTCTC 3 cut(s) 217, 237, 1515
FalI AAGNNNNNCTT 2 cut(s) 2217, 2249
FaqI GGGAC 1 cut(s) 1776
FauI CCCGC 1 cut(s) 1125
FauNDI CATATG 1 cut(s) 954
FbaI TGATCA 3 cut(s) 1476, 1672, 2236
FriOI GRGCYC 4 cut(s) 787, 1406, 1663, 2346
FspBI CTAG 2 cut(s) 2006, 2243
GlaI GCGC 3 cut(s) 235, 328, 2583
GsaI CCCAGC 2 cut(s) 866, 1723
HaeIII GGCC 8 cut(s) 59, 499, 569, 581, 590, 1248, 2286, 2565
HgaI GACGC 4 cut(s) 688, 1303, 1592, 2565
HhaI GCGC 3 cut(s) 236, 329, 2584
Hin1I GRCGYC 2 cut(s) 333, 396
Hin6I GCGC 3 cut(s) 234, 327, 2582
HinP1I GCGC 3 cut(s) 234, 327, 2582
HincII GTYRAC 1 cut(s) 460
HindII GTYRAC 1 cut(s) 460
HindIII AAGCTT 2 cut(s) 704, 1778
HphI GGTGA 5 cut(s) 1445, 2026, 2231, 2367, 2380
Hpy166II GTNNAC 7 cut(s) 460, 542, 557, 1383, 1876, 2171, 2320
Hpy8I GTNNAC 7 cut(s) 460, 542, 557, 1383, 1876, 2171, 2320
Hpy99I CGWCG 8 cut(s) 128, 338, 398, 401, 451, 739, 742, 1297
HpyAV CCTTC 5 cut(s) 601, 1000, 1009, 1132, 1960
HpyCH4III ACNGT 7 cut(s) 131, 539, 554, 1387, 2168, 2494, 2539
HpyCH4IV ACGT 7 cut(s) 174, 333, 348, 396, 573, 636, 1379
HpyF10VI GCNNNNNNNGC 4 cut(s) 324, 587, 1316, 1658
HpyF3I CTNAG 5 cut(s) 779, 1514, 1863, 1976, 2410
HpySE526I ACGT 7 cut(s) 174, 333, 348, 396, 573, 636, 1379
Hsp92I GRCGYC 2 cut(s) 333, 396
HspAI GCGC 3 cut(s) 234, 327, 2582
KflI GGGWCCC 1 cut(s) 1763
Kpn2I TCCGGA 2 cut(s) 1773, 2608
KroI GCCGGC 1 cut(s) 1824
KroNI GCCGGC 1 cut(s) 1826
Ksp22I TGATCA 3 cut(s) 1476, 1672, 2236
LguI GCTCTTC 1 cut(s) 1110
LmnI GCTCC 7 cut(s) 365, 1401, 1520, 1888, 2075, 2119, 2341
Lsp1109I GCAGC 7 cut(s) 504, 1117, 1294, 1454, 2094, 2144, 2217
LweI GCATC 8 cut(s) 1129, 1540, 1552, 1706, 1734, 2182, 2191, 2442
MabI ACCWGGT 1 cut(s) 2036
MaeI CTAG 2 cut(s) 2006, 2243
MaeII ACGT 7 cut(s) 174, 333, 348, 396, 573, 636, 1379
MaeIII GTNAC 3 cut(s) 1288, 2027, 2373
MflI RGATCY 4 cut(s) 11, 69, 642, 1678
MhlI GDGCHC 7 cut(s) 544, 559, 787, 1406, 1663, 2173, 2346
MlsI TGGCCA 1 cut(s) 1248
MluCI AATT 6 cut(s) 31, 793, 1363, 1490, 1570, 1912
MluNI TGGCCA 1 cut(s) 1248
MlyI GAGTC 1 cut(s) 87
MmeI TCCRAC 8 cut(s) 47, 147, 177, 240, 325, 720, 1569, 2072
Mox20I TGGCCA 1 cut(s) 1248
Mph1103I ATGCAT 1 cut(s) 2396
MroI TCCGGA 2 cut(s) 1773, 2608
MroNI GCCGGC 1 cut(s) 1824
MscI TGGCCA 1 cut(s) 1248
MseI TTAA 6 cut(s) 359, 930, 1071, 1341, 2277, 2427
MslI CAYNNNNRTG 2 cut(s) 1583, 2190
Msp20I TGGCCA 1 cut(s) 1248
Mva1269I GAATGC 1 cut(s) 864
MvaI CCWGG 3 cut(s) 1540, 2038, 2219
MvnI CGCG 4 cut(s) 234, 236, 329, 699
MwoI GCNNNNNNNGC 4 cut(s) 324, 587, 1316, 1658
NaeI GCCGGC 1 cut(s) 1826
NciI CCSGG 7 cut(s) 56, 93, 623, 767, 1166, 1254, 1255
NdeI CATATG 1 cut(s) 954
NgoMIV GCCGGC 1 cut(s) 1824
NlaIV GGNNCC 9 cut(s) 39, 71, 367, 644, 812, 1371, 1764, 1765, 2121
NmeAIII GCCGAG 2 cut(s) 394, 1202
NmuCI GTSAC 2 cut(s) 1288, 2373
NsiI ATGCAT 1 cut(s) 2396
NspI RCATGY 1 cut(s) 517
NspV TTCGAA 1 cut(s) 273
PaeR7I CTCGAG 1 cut(s) 7
PagI TCATGA 1 cut(s) 1642
PceI AGGCCT 3 cut(s) 569, 590, 2565
PciSI GCTCTTC 1 cut(s) 1110
PcsI WCGNNNNNNNCGW 4 cut(s) 238, 381, 550, 2442
PctI GAATGC 1 cut(s) 864
PdiI GCCGGC 1 cut(s) 1826
PfeI GAWTC 9 cut(s) 204, 603, 757, 775, 949, 1743, 1970, 2051, 2447
PflFI GACNNNGTC 1 cut(s) 395
PflMI CCANNNNNTGG 3 cut(s) 264, 661, 2594
PfoI TCCNGGA 1 cut(s) 765
PleI GAGTC 1 cut(s) 86
PpsI GAGTC 1 cut(s) 86
Ppu21I YACGTR 3 cut(s) 349, 574, 1380
PpuMI RGGWCCY 1 cut(s) 1763
PshAI GACNNNNGTC 1 cut(s) 452
Psp124BI GAGCTC 2 cut(s) 1406, 2346
Psp1406I AACGTT 1 cut(s) 174
Psp5II RGGWCCY 1 cut(s) 1763
Psp6I CCWGG 3 cut(s) 1538, 2036, 2217
PspFI CCCAGC 2 cut(s) 862, 1719
PspGI CCWGG 3 cut(s) 1538, 2036, 2217
PspN4I GGNNCC 9 cut(s) 39, 71, 367, 644, 812, 1371, 1764, 1765, 2121
PspPI GGNCC 6 cut(s) 37, 57, 295, 1283, 1763, 2597
PspPPI RGGWCCY 1 cut(s) 1763
PsuI RGATCY 4 cut(s) 11, 69, 642, 1678
PsyI GACNNNGTC 1 cut(s) 395
RsaI GTAC 4 cut(s) 347, 692, 893, 1382
RsaNI GTAC 4 cut(s) 346, 691, 892, 1381
RseI CAYNNNNRTG 2 cut(s) 1583, 2190
SacI GAGCTC 2 cut(s) 1406, 2346
SapI GCTCTTC 1 cut(s) 1110
SaqAI TTAA 6 cut(s) 359, 930, 1071, 1341, 2277, 2427
Sau96I GGNCC 6 cut(s) 37, 57, 295, 1283, 1763, 2597
SchI GAGTC 1 cut(s) 87
SduI GDGCHC 7 cut(s) 544, 559, 787, 1406, 1663, 2173, 2346
SexAI ACCWGGT 1 cut(s) 2036
SfaNI GCATC 8 cut(s) 1129, 1540, 1552, 1706, 1734, 2182, 2191, 2442
SfcI CTRYAG 1 cut(s) 850
SfiI GGCCNNNNNGGCC 1 cut(s) 587
Sfr274I CTCGAG 1 cut(s) 7
SfuI TTCGAA 1 cut(s) 273
SinI GGWCC 5 cut(s) 37, 295, 1283, 1763, 2597
SlaI CTCGAG 1 cut(s) 7
SmaI CCCGGG 1 cut(s) 1255
SmiMI CAYNNNNRTG 2 cut(s) 1583, 2190
SmlI CTYRAG 3 cut(s) 7, 679, 1682
SmoI CTYRAG 3 cut(s) 7, 679, 1682
Sse9I AATT 6 cut(s) 31, 793, 1363, 1490, 1570, 1912
SseBI AGGCCT 3 cut(s) 569, 590, 2565
SsiI CCGC 7 cut(s) 138, 315, 318, 329, 697, 1132, 2600
SspMI CTAG 2 cut(s) 2006, 2243
SstI GAGCTC 2 cut(s) 1406, 2346
StuI AGGCCT 3 cut(s) 569, 590, 2565
StyI CCWWGG 1 cut(s) 2057
TaaI ACNGT 7 cut(s) 131, 539, 554, 1387, 2168, 2494, 2539
TaiI ACGT 7 cut(s) 177, 336, 351, 399, 576, 639, 1382
TaqI TCGA 7 cut(s) 8, 241, 273, 336, 375, 916, 1278
TasI AATT 6 cut(s) 31, 793, 1363, 1490, 1570, 1912
TauI GCSGC 3 cut(s) 141, 318, 321
TfiI GAWTC 9 cut(s) 204, 603, 757, 775, 949, 1743, 1970, 2051, 2447
Tru1I TTAA 6 cut(s) 359, 930, 1071, 1341, 2277, 2427
Tru9I TTAA 6 cut(s) 359, 930, 1071, 1341, 2277, 2427
TseFI GTSAC 2 cut(s) 1288, 2373
TseI GCWGC 7 cut(s) 517, 1105, 1307, 1442, 2107, 2132, 2230
Tsp45I GTSAC 2 cut(s) 1288, 2373
TspDTI ATGAA 8 cut(s) 88, 860, 941, 1115, 1376, 1583, 1936, 2617
TspGWI ACGGA 6 cut(s) 378, 464, 723, 1497, 1517, 2445
TspMI CCCGGG 1 cut(s) 1253
Tth111I GACNNNGTC 1 cut(s) 395
Van91I CCANNNNNTGG 3 cut(s) 264, 661, 2594
VneI GTGCAC 3 cut(s) 540, 555, 2169
VpaK11BI GGWCC 5 cut(s) 37, 295, 1283, 1763, 2597
XapI RAATTY 4 cut(s) 31, 1363, 1490, 1570
XceI RCATGY 1 cut(s) 517
XhoI CTCGAG 1 cut(s) 7
XmaI CCCGGG 1 cut(s) 1253
XspI CTAG 2 cut(s) 2006, 2243
ZraI GACGTC 2 cut(s) 334, 397
Zsp2I ATGCAT 1 cut(s) 2396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.