FvH4_4g12911

Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one- codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0018831
Physical Location & Seq
Forward (+)
16573977 .. 16575546
1570 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g12911.t1

Sequence Viewer

Length: 591 bp
ATGCTTTTGGTGGCCCAACAGCCCCAGCTGAACCTGTTCCTTTGGCAGGAGTATGCTATTTCGGTTTTACAAGAACGGCAGACAAAAATTGATGTTTTGAATCAGAAGTTGGTGGAAAAAGAAAGGGAGTTGGAGGTAAAAGAAGTCGCATTGGATAAGCAATCGAAGGATTTGGCGTTGCTGGCAGCCGATATCGAAAAGATTAACAACTTGGAGATAACAAGTTTGCCGTTTGCAACCGATGCTGTAAAGGTGGTTGAGATGGAGGAGAAGAAGAAAGGGGAAGAGACACCTCTTTTGCGAGATATGGTGATGAAGAAGAACTTGCTGGTTGGGATTGGGGTTAGTGTGGTTTCGATTATTCTAGTGTTAAGCTTGATTGAGATTGTGTTGAGTTGCAAGAAGCAACCTTGGTGGAATAGTGAAGAGAACGCTGTGGATTTGAGCCGGTACCTAATTGAGAGGATAAGGAACTTCTCCATTATCGCCCATGTCGATCACGACAAGTCTACTCTCGTTGATTGGCTCCTGGAGCTCACTGGCACTATTAAGAAAGGCCATGGATTGGTGGATGTTATAGCTATTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EFG_C GTP_EFTU GTP_EFTU_D2 LepA_C

Protein Analysis

197

Amino Acids

22.29

Weight (kDa)

5.22

Isoelectric Point (pI)

36.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 154 - 186 3.6e-07 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018831)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g12911
pyrus_communis pycom111g03920
rosa_laevigata RLG00000020534 RLG00000023537 RLG00000028425 RLG00000035877
rosa_samantha Rh2AG244200 Rh2AG539500 Rh2DG253000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 450
AccB1I GGYRCC 1 cut(s) 450
AccB7I CCANNNNNTGG 1 cut(s) 565
AccI GTMKAC 1 cut(s) 509
AfaI GTAC 1 cut(s) 452
AfiI CCNNNNNNNGG 2 cut(s) 46, 565
AgsI TTSAA 1 cut(s) 100
AjnI CCWGG 1 cut(s) 528
AjuI GAANNNNNNNTTGG 2 cut(s) 92, 124
AluBI AGCT 4 cut(s) 28, 375, 535, 581
AluI AGCT 4 cut(s) 28, 375, 535, 581
Alw21I GWGCWC 1 cut(s) 537
Alw26I GTCTC 1 cut(s) 281
AoxI GGCC 2 cut(s) 12, 556
ApeKI GCWGC 1 cut(s) 185
ArsI GACNNNNNNTTYG 2 cut(s) 280, 312
Asp700I GAANNNNTTC 1 cut(s) 35
Asp718I GGTACC 1 cut(s) 450
AspS9I GGNCC 1 cut(s) 13
AsuHPI GGTGA 1 cut(s) 322
BanI GGYRCC 1 cut(s) 450
BanII GRGCYC 1 cut(s) 537
Bbv12I GWGCWC 1 cut(s) 537
BbvI GCAGC 1 cut(s) 197
BccI CCATC 1 cut(s) 256
BceAI ACGGC 2 cut(s) 92, 214
BciT130I CCWGG 1 cut(s) 530
BcoDI GTCTC 1 cut(s) 281
BfaI CTAG 1 cut(s) 365
BisI GCNGC 1 cut(s) 186
BlsI GCNGC 1 cut(s) 187
Bme1390I CCNGG 1 cut(s) 530
BmgT120I GGNCC 1 cut(s) 13
BmiI GGNNCC 2 cut(s) 452, 527
BmrFI CCNGG 1 cut(s) 530
BmsI GCATC 1 cut(s) 232
BpmI CTGGAG 1 cut(s) 551
BsaJI CCNNGG 2 cut(s) 410, 559
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 565
Bse118I RCCGGY 1 cut(s) 447
Bse1I ACTGG 1 cut(s) 544
BseBI CCWGG 1 cut(s) 530
BseDI CCNNGG 2 cut(s) 410, 559
BseGI GGATG 1 cut(s) 577
BseLI CCNNNNNNNGG 2 cut(s) 46, 565
BseNI ACTGG 1 cut(s) 544
BseRI GAGGAG 1 cut(s) 281
BseXI GCAGC 1 cut(s) 197
BseYI CCCAGC 1 cut(s) 24
BshFI GGCC 2 cut(s) 14, 558
BshNI GGYRCC 1 cut(s) 450
BsiHKAI GWGCWC 1 cut(s) 537
BsiSI CCGG 1 cut(s) 448
BslI CCNNNNNNNGG 2 cut(s) 46, 565
BsmAI GTCTC 1 cut(s) 281
BsnI GGCC 2 cut(s) 14, 558
Bsp1286I GDGCHC 1 cut(s) 537
Bsp143I GATC 1 cut(s) 496
Bsp19I CCATGG 1 cut(s) 559
BspANI GGCC 2 cut(s) 14, 558
BspLI GGNNCC 2 cut(s) 452, 527
BspT107I GGYRCC 1 cut(s) 450
BsrFI RCCGGY 1 cut(s) 447
BsrI ACTGG 1 cut(s) 544
BssAI RCCGGY 1 cut(s) 447
BssECI CCNNGG 2 cut(s) 410, 559
BssMI GATC 1 cut(s) 496
BssT1I CCWWGG 2 cut(s) 410, 559
Bst2UI CCWGG 1 cut(s) 530
Bst6I CTCTTC 2 cut(s) 279, 420
BstAPI GCANNNNNTGC 1 cut(s) 242
BstC8I GCNNGC 1 cut(s) 183
BstDSI CCRYGG 1 cut(s) 559
BstENI CCTNNNNNAGG 1 cut(s) 44
BstF5I GGATG 1 cut(s) 577
BstKTI GATC 1 cut(s) 499
BstMAI GTCTC 1 cut(s) 281
BstMBI GATC 1 cut(s) 496
BstMWI GCNNNNNNNGC 3 cut(s) 182, 242, 532
BstNI CCWGG 1 cut(s) 530
BstSCI CCNGG 1 cut(s) 528
BstV1I GCAGC 1 cut(s) 197
BsuRI GGCC 2 cut(s) 14, 558
BtgI CCRYGG 1 cut(s) 559
BtsCI GGATG 1 cut(s) 577
BtsIMutI CAGTG 1 cut(s) 537
Cac8I GCNNGC 1 cut(s) 183
Cfr10I RCCGGY 1 cut(s) 447
Cfr13I GGNCC 1 cut(s) 13
Csp6I GTAC 1 cut(s) 451
CspCI CAANNNNNGTGG 2 cut(s) 395, 430
CviAII CATG 2 cut(s) 491, 560
CviQI GTAC 1 cut(s) 451
DpnI GATC 1 cut(s) 498
DpnII GATC 1 cut(s) 496
Eam1104I CTCTTC 2 cut(s) 279, 420
EarI CTCTTC 2 cut(s) 279, 420
Ecl136II GAGCTC 1 cut(s) 535
Eco130I CCWWGG 2 cut(s) 410, 559
Eco24I GRGCYC 1 cut(s) 537
Eco32I GATATC 1 cut(s) 193
Eco53kI GAGCTC 1 cut(s) 535
EcoICRI GAGCTC 1 cut(s) 535
EcoNI CCTNNNNNAGG 1 cut(s) 44
EcoRII CCWGG 1 cut(s) 528
EcoRV GATATC 1 cut(s) 193
EcoT14I CCWWGG 2 cut(s) 410, 559
EcoT38I GRGCYC 1 cut(s) 537
ErhI CCWWGG 2 cut(s) 410, 559
FaeI CATG 2 cut(s) 494, 563
FaiI YATR 5 cut(s) 54, 308, 492, 561, 578
FalI AAGNNNNNCTT 2 cut(s) 308, 340
FatI CATG 2 cut(s) 490, 559
FblI GTMKAC 1 cut(s) 509
Fnu4HI GCNGC 1 cut(s) 186
FokI GGATG 1 cut(s) 584
FriOI GRGCYC 1 cut(s) 537
Fsp4HI GCNGC 1 cut(s) 186
FspBI CTAG 1 cut(s) 365
GluI GCNGC 1 cut(s) 186
GsaI CCCAGC 1 cut(s) 28
GsuI CTGGAG 1 cut(s) 551
HaeIII GGCC 2 cut(s) 14, 558
HapII CCGG 1 cut(s) 448
Hin1II CATG 2 cut(s) 494, 563
HindIII AAGCTT 1 cut(s) 373
HinfI GANTC 1 cut(s) 100
HpaII CCGG 1 cut(s) 448
HphI GGTGA 1 cut(s) 322
Hpy166II GTNNAC 1 cut(s) 510
Hpy188I TCNGA 1 cut(s) 105
Hpy188III TCNNGA 1 cut(s) 500
Hpy8I GTNNAC 1 cut(s) 510
HpyAV CCTTC 1 cut(s) 160
HpyCH4V TGCA 2 cut(s) 236, 399
HpyF10VI GCNNNNNNNGC 3 cut(s) 182, 242, 532
Hsp92II CATG 2 cut(s) 494, 563
KpnI GGTACC 1 cut(s) 454
Kzo9I GATC 1 cut(s) 496
LmnI GCTCC 2 cut(s) 531, 532
LpnPI CCDG 9 cut(s) 32, 38, 47, 167, 314, 461, 515, 525, 542
Lsp1109I GCAGC 1 cut(s) 197
LweI GCATC 1 cut(s) 232
MaeI CTAG 1 cut(s) 365
MalI GATC 1 cut(s) 498
MboI GATC 1 cut(s) 496
MboII GAAGA 6 cut(s) 283, 286, 296, 328, 331, 437
MhlI GDGCHC 1 cut(s) 537
MluCI AATT 2 cut(s) 87, 456
MmeI TCCRAC 1 cut(s) 111
MnlI CCTC 4 cut(s) 127, 259, 303, 456
MroXI GAANNNNTTC 1 cut(s) 35
MseI TTAA 3 cut(s) 204, 371, 549
MspA1I CMGCKG 1 cut(s) 28
MspI CCGG 1 cut(s) 448
MspR9I CCNGG 1 cut(s) 530
MvaI CCWGG 1 cut(s) 530
MwoI GCNNNNNNNGC 3 cut(s) 182, 242, 532
NcoI CCATGG 1 cut(s) 559
NdeII GATC 1 cut(s) 496
NlaIII CATG 2 cut(s) 494, 563
NlaIV GGNNCC 2 cut(s) 452, 527
PcsI WCGNNNNNNNCGW 1 cut(s) 492
PdmI GAANNNNTTC 1 cut(s) 35
PfeI GAWTC 1 cut(s) 100
PflMI CCANNNNNTGG 1 cut(s) 565
PfoI TCCNGGA 1 cut(s) 528
PkrI GCNGC 1 cut(s) 187
Psp124BI GAGCTC 1 cut(s) 537
Psp6I CCWGG 1 cut(s) 528
PspFI CCCAGC 1 cut(s) 24
PspGI CCWGG 1 cut(s) 528
PspN4I GGNNCC 2 cut(s) 452, 527
PspPI GGNCC 1 cut(s) 13
PvuII CAGCTG 1 cut(s) 28
RsaI GTAC 1 cut(s) 452
RsaNI GTAC 1 cut(s) 451
SacI GAGCTC 1 cut(s) 537
SaqAI TTAA 3 cut(s) 204, 371, 549
SatI GCNGC 1 cut(s) 186
Sau3AI GATC 1 cut(s) 496
Sau96I GGNCC 1 cut(s) 13
ScrFI CCNGG 1 cut(s) 530
SduI GDGCHC 1 cut(s) 537
SfaNI GCATC 1 cut(s) 232
Sse9I AATT 2 cut(s) 87, 456
SspMI CTAG 1 cut(s) 365
SstI GAGCTC 1 cut(s) 537
StyD4I CCNGG 1 cut(s) 528
StyI CCWWGG 2 cut(s) 410, 559
TaqI TCGA 4 cut(s) 164, 195, 356, 495
TasI AATT 2 cut(s) 87, 456
TfiI GAWTC 1 cut(s) 100
Tru1I TTAA 3 cut(s) 204, 371, 549
Tru9I TTAA 3 cut(s) 204, 371, 549
TscAI CASTG 1 cut(s) 544
TseI GCWGC 1 cut(s) 185
TspDTI ATGAA 1 cut(s) 329
TspRI CASTG 1 cut(s) 544
Van91I CCANNNNNTGG 1 cut(s) 565
XagI CCTNNNNNAGG 1 cut(s) 44
XmiI GTMKAC 1 cut(s) 509
XmnI GAANNNNTTC 1 cut(s) 35
XspI CTAG 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.