Rh2DG253000

Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one- codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0018831
Physical Location & Seq
Forward (+)
26311585 .. 26312019
435 bp
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UTR
Exon/CDS
Intron
Rh2DG253000.1

Sequence Viewer

Length: 435 bp
ATGGGTTTTCTCAGCAGAGCCTCTAAAACCCTGAAACCACCAAAGAACCTATCTTTACTACAAACCCACCTTACCTTAAACCCTTTATCCTCTATTTTCAGACGCAACCCAGTTTCCACCAACTTCAACTACGACCCTTTGAGATTGAGCCAAGCTTTTTGCTCCCATTCTCGCCAAAGCAGCAAAGAAAACGCCGTGGATTTGAGCCAGTACCCTGCTGAGAGGATCAGGAACTTCTCCATTATCGCCCATGTCGATCACGGCAAGTCTACTCTGGCAGATAGGCTGTTGGAGCTCACTGGGACCATTAAGAGAGGCCATGGTCAGCCTCAGTACCTTGATAAATTGCAGGTATATGAAACTTTAATGATTGTTAAAAGACATAGTGGCATTGAAACCAGCTCCCACCAAGCATATAGATCATTACATTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EFG_C GTP_EFTU GTP_EFTU_D2 LepA_C

Protein Analysis

144

Amino Acids

16.4

Weight (kDa)

10.06

Isoelectric Point (pI)

36.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 74 - 116 5.7e-11 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0018831)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g12911
pyrus_communis pycom111g03920
rosa_laevigata RLG00000020534 RLG00000023537 RLG00000028425 RLG00000035877
rosa_samantha Rh2AG244200 Rh2AG539500 Rh2DG253000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 340
AccI GTMKAC 1 cut(s) 269
AclWI GGATC 1 cut(s) 233
AfaI GTAC 2 cut(s) 212, 335
AgsI TTSAA 2 cut(s) 127, 395
AluBI AGCT 3 cut(s) 155, 295, 402
AluI AGCT 3 cut(s) 155, 295, 402
Alw21I GWGCWC 1 cut(s) 297
AlwI GGATC 1 cut(s) 233
AoxI GGCC 1 cut(s) 316
ApeKI GCWGC 1 cut(s) 180
AspS9I GGNCC 1 cut(s) 303
AvaII GGWCC 1 cut(s) 303
BanII GRGCYC 1 cut(s) 297
Bbv12I GWGCWC 1 cut(s) 297
BbvI GCAGC 1 cut(s) 192
BceAI ACGGC 2 cut(s) 179, 277
BfaI CTAG 1 cut(s) 433
BfuAI ACCTGC 1 cut(s) 340
BisI GCNGC 1 cut(s) 181
BlsI GCNGC 1 cut(s) 182
Bme18I GGWCC 1 cut(s) 303
BmgT120I GGNCC 1 cut(s) 303
BmiI GGNNCC 1 cut(s) 304
BmrI ACTGGG 2 cut(s) 104, 309
BmuI ACTGGG 2 cut(s) 104, 309
BsaJI CCNNGG 2 cut(s) 195, 319
Bse1I ACTGG 3 cut(s) 110, 208, 304
BseDI CCNNGG 2 cut(s) 195, 319
BseMII CTCAG 3 cut(s) 25, 210, 344
BseNI ACTGG 3 cut(s) 110, 208, 304
BseXI GCAGC 1 cut(s) 192
BshFI GGCC 1 cut(s) 318
BsiHKAI GWGCWC 1 cut(s) 297
BslFI GGGAC 1 cut(s) 316
BsmFI GGGAC 1 cut(s) 316
BsnI GGCC 1 cut(s) 318
Bsp1286I GDGCHC 1 cut(s) 297
Bsp143I GATC 3 cut(s) 225, 256, 419
Bsp19I CCATGG 1 cut(s) 319
BspANI GGCC 1 cut(s) 318
BspCNI CTCAG 3 cut(s) 24, 211, 343
BspLI GGNNCC 1 cut(s) 304
BspMI ACCTGC 1 cut(s) 340
BspPI GGATC 1 cut(s) 233
BsrI ACTGG 3 cut(s) 110, 208, 304
BssECI CCNNGG 2 cut(s) 195, 319
BssMI GATC 3 cut(s) 225, 256, 419
BssT1I CCWWGG 1 cut(s) 319
BstDEI CTNAG 3 cut(s) 11, 219, 330
BstDSI CCRYGG 2 cut(s) 195, 319
BstKTI GATC 3 cut(s) 228, 259, 422
BstMBI GATC 3 cut(s) 225, 256, 419
BstMWI GCNNNNNNNGC 2 cut(s) 180, 292
BstV1I GCAGC 1 cut(s) 192
BsuRI GGCC 1 cut(s) 318
BtgI CCRYGG 2 cut(s) 195, 319
BtsIMutI CAGTG 1 cut(s) 297
BveI ACCTGC 1 cut(s) 340
Cfr13I GGNCC 1 cut(s) 303
CseI GACGC 1 cut(s) 111
Csp6I GTAC 2 cut(s) 211, 334
CviAII CATG 2 cut(s) 251, 320
CviJI RGCY 9 cut(s) 20, 150, 155, 207, 286, 295, 318, 328, 402
CviKI_1 RGCY 9 cut(s) 20, 150, 155, 207, 286, 295, 318, 328, 402
CviQI GTAC 2 cut(s) 211, 334
DdeI CTNAG 3 cut(s) 11, 219, 330
DpnI GATC 3 cut(s) 227, 258, 421
DpnII GATC 3 cut(s) 225, 256, 419
Ecl136II GAGCTC 1 cut(s) 295
Eco130I CCWWGG 1 cut(s) 319
Eco24I GRGCYC 1 cut(s) 297
Eco47I GGWCC 1 cut(s) 303
Eco53kI GAGCTC 1 cut(s) 295
EcoICRI GAGCTC 1 cut(s) 295
EcoT14I CCWWGG 1 cut(s) 319
EcoT38I GRGCYC 1 cut(s) 297
ErhI CCWWGG 1 cut(s) 319
FaeI CATG 2 cut(s) 254, 323
FaiI YATR 7 cut(s) 252, 321, 355, 357, 384, 415, 417
FaqI GGGAC 1 cut(s) 316
FatI CATG 2 cut(s) 250, 319
FblI GTMKAC 1 cut(s) 269
Fnu4HI GCNGC 1 cut(s) 181
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 1 cut(s) 181
FspBI CTAG 1 cut(s) 433
GluI GCNGC 1 cut(s) 181
HaeIII GGCC 1 cut(s) 318
HgaI GACGC 1 cut(s) 111
Hin1II CATG 2 cut(s) 254, 323
HindIII AAGCTT 1 cut(s) 153
Hpy166II GTNNAC 1 cut(s) 270
Hpy188I TCNGA 1 cut(s) 101
Hpy188III TCNNGA 1 cut(s) 229
Hpy8I GTNNAC 1 cut(s) 270
HpyCH4V TGCA 1 cut(s) 349
HpyF10VI GCNNNNNNNGC 2 cut(s) 180, 292
HpyF3I CTNAG 3 cut(s) 11, 219, 330
Hsp92II CATG 2 cut(s) 254, 323
Kzo9I GATC 3 cut(s) 225, 256, 419
LmnI GCTCC 3 cut(s) 167, 292, 407
LpnPI CCDG 9 cut(s) 44, 123, 214, 221, 228, 260, 285, 335, 412
Lsp1109I GCAGC 1 cut(s) 192
MaeI CTAG 1 cut(s) 433
MalI GATC 3 cut(s) 227, 258, 421
MboI GATC 3 cut(s) 225, 256, 419
MhlI GDGCHC 1 cut(s) 297
MluCI AATT 1 cut(s) 344
MmeI TCCRAC 1 cut(s) 270
MnlI CCTC 5 cut(s) 31, 100, 216, 308, 339
MseI TTAA 4 cut(s) 77, 309, 365, 375
MwoI GCNNNNNNNGC 2 cut(s) 180, 292
NcoI CCATGG 1 cut(s) 319
NdeII GATC 3 cut(s) 225, 256, 419
NlaIII CATG 2 cut(s) 254, 323
NlaIV GGNNCC 1 cut(s) 304
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PkrI GCNGC 1 cut(s) 182
Psp124BI GAGCTC 1 cut(s) 297
PspN4I GGNNCC 1 cut(s) 304
PspPI GGNCC 1 cut(s) 303
RsaI GTAC 2 cut(s) 212, 335
RsaNI GTAC 2 cut(s) 211, 334
SacI GAGCTC 1 cut(s) 297
SaqAI TTAA 4 cut(s) 77, 309, 365, 375
SatI GCNGC 1 cut(s) 181
Sau3AI GATC 3 cut(s) 225, 256, 419
Sau96I GGNCC 1 cut(s) 303
SduI GDGCHC 1 cut(s) 297
SetI ASST 8 cut(s) 51, 72, 77, 157, 297, 339, 354, 404
SinI GGWCC 1 cut(s) 303
Sse9I AATT 1 cut(s) 344
SspMI CTAG 1 cut(s) 433
SstI GAGCTC 1 cut(s) 297
StyI CCWWGG 1 cut(s) 319
TaqI TCGA 1 cut(s) 255
TasI AATT 1 cut(s) 344
Tru1I TTAA 4 cut(s) 77, 309, 365, 375
Tru9I TTAA 4 cut(s) 77, 309, 365, 375
TscAI CASTG 1 cut(s) 304
TseI GCWGC 1 cut(s) 180
TspDTI ATGAA 1 cut(s) 372
TspRI CASTG 1 cut(s) 304
VpaK11BI GGWCC 1 cut(s) 303
XmiI GTMKAC 1 cut(s) 269
XspI CTAG 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.