Rh2AG244200

Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one- codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0018831
Physical Location & Seq
Forward (+)
27003030 .. 27005427
2398 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG244200.1

Sequence Viewer

Length: 492 bp
ATGGGTTTTCTCAGCAGAGCCTCTAAAACCCTGAAACCACCAAAGAACCTATCTTTACTACAAACCCACCTTACCTTAAACCCTTTATCCTCTATTTTCAGACGCAACCCAGTTTCCACCAACTTCAACTACGACCCTTTGAGATTGAGCCAAGCTTTTTGCTCCCATTCTCGCCAAAGCAGCAAAGAAAACGCCGTGGATTTGAGCCAGTACCCTGCTGAGAGGATCAGGAACTTCTCCATTATCGCCCATGTCGATCACGGCAAGTCTACTCTGGCAGATAGGCTGTTGGAGCTCACTGGGACCATTAAGAGAGGCCATGGTCAGCCTCAGTACCTTGATAAATTGCAGGTACCTGTAAAGTATTTGATTGGGCTTGAAAGGAAAAGCGCAAGACAAGTCTTCTTGGTTCGTCTTTCTTGGAGCATGTTAAGGAGATATTGGAACCTGATACTTTTATTGCTGCAGCGCCTTGGGTGTACTACCCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EFG_C GTP_EFTU GTP_EFTU_D2 LepA_C

Protein Analysis

163

Amino Acids

18.74

Weight (kDa)

10.64

Isoelectric Point (pI)

39.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 74 - 115 1e-10 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018831)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g12911
pyrus_communis pycom111g03920
rosa_laevigata RLG00000020534 RLG00000023537 RLG00000028425 RLG00000035877
rosa_samantha Rh2AG244200 Rh2AG539500 Rh2DG253000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 340
Acc65I GGTACC 1 cut(s) 352
AccB1I GGYRCC 1 cut(s) 352
AccI GTMKAC 1 cut(s) 269
AclWI GGATC 1 cut(s) 233
AfaI GTAC 4 cut(s) 212, 335, 354, 481
AgsI TTSAA 2 cut(s) 127, 380
AluBI AGCT 2 cut(s) 155, 295
AluI AGCT 2 cut(s) 155, 295
Alw21I GWGCWC 1 cut(s) 297
AlwI GGATC 1 cut(s) 233
AoxI GGCC 1 cut(s) 316
ApeKI GCWGC 3 cut(s) 180, 463, 466
Asp718I GGTACC 1 cut(s) 352
AspLEI GCGC 2 cut(s) 392, 471
AspS9I GGNCC 1 cut(s) 303
AvaII GGWCC 1 cut(s) 303
BanI GGYRCC 1 cut(s) 352
BanII GRGCYC 1 cut(s) 297
BbsI GAAGAC 1 cut(s) 394
Bbv12I GWGCWC 1 cut(s) 297
BbvI GCAGC 3 cut(s) 192, 450, 478
BceAI ACGGC 2 cut(s) 179, 277
BfmI CTRYAG 1 cut(s) 464
BfoI RGCGCY 1 cut(s) 472
BfuAI ACCTGC 1 cut(s) 340
BisI GCNGC 3 cut(s) 181, 464, 467
BlsI GCNGC 3 cut(s) 182, 465, 468
Bme18I GGWCC 1 cut(s) 303
BmgT120I GGNCC 1 cut(s) 303
BmiI GGNNCC 3 cut(s) 304, 354, 446
BmrI ACTGGG 2 cut(s) 104, 309
BmuI ACTGGG 2 cut(s) 104, 309
BpiI GAAGAC 1 cut(s) 394
BsaJI CCNNGG 3 cut(s) 195, 319, 472
Bse1I ACTGG 3 cut(s) 110, 208, 304
BseDI CCNNGG 3 cut(s) 195, 319, 472
BseMII CTCAG 3 cut(s) 25, 210, 344
BseNI ACTGG 3 cut(s) 110, 208, 304
BseXI GCAGC 3 cut(s) 192, 450, 478
BshFI GGCC 1 cut(s) 318
BshNI GGYRCC 1 cut(s) 352
BsiHKAI GWGCWC 1 cut(s) 297
BslFI GGGAC 1 cut(s) 316
BsmFI GGGAC 1 cut(s) 316
BsnI GGCC 1 cut(s) 318
Bsp1286I GDGCHC 1 cut(s) 297
Bsp143I GATC 2 cut(s) 225, 256
Bsp19I CCATGG 1 cut(s) 319
BspANI GGCC 1 cut(s) 318
BspCNI CTCAG 3 cut(s) 24, 211, 343
BspLI GGNNCC 3 cut(s) 304, 354, 446
BspMAI CTGCAG 1 cut(s) 468
BspMI ACCTGC 1 cut(s) 340
BspPI GGATC 1 cut(s) 233
BspT107I GGYRCC 1 cut(s) 352
BsrI ACTGG 3 cut(s) 110, 208, 304
BssECI CCNNGG 3 cut(s) 195, 319, 472
BssMI GATC 2 cut(s) 225, 256
BssT1I CCWWGG 2 cut(s) 319, 472
BstDEI CTNAG 3 cut(s) 11, 219, 330
BstDSI CCRYGG 2 cut(s) 195, 319
BstH2I RGCGCY 1 cut(s) 472
BstHHI GCGC 2 cut(s) 392, 471
BstKTI GATC 2 cut(s) 228, 259
BstMBI GATC 2 cut(s) 225, 256
BstMWI GCNNNNNNNGC 2 cut(s) 180, 292
BstNSI RCATGY 1 cut(s) 430
BstSFI CTRYAG 1 cut(s) 464
BstV1I GCAGC 3 cut(s) 192, 450, 478
BstV2I GAAGAC 1 cut(s) 394
BsuRI GGCC 1 cut(s) 318
BtgI CCRYGG 2 cut(s) 195, 319
BtsIMutI CAGTG 1 cut(s) 297
BveI ACCTGC 1 cut(s) 340
CfoI GCGC 2 cut(s) 392, 471
Cfr13I GGNCC 1 cut(s) 303
CseI GACGC 1 cut(s) 111
Csp6I GTAC 4 cut(s) 211, 334, 353, 480
CviAII CATG 3 cut(s) 251, 320, 427
CviJI RGCY 9 cut(s) 20, 150, 155, 207, 286, 295, 318, 328, 376
CviKI_1 RGCY 9 cut(s) 20, 150, 155, 207, 286, 295, 318, 328, 376
CviQI GTAC 4 cut(s) 211, 334, 353, 480
DdeI CTNAG 3 cut(s) 11, 219, 330
DpnI GATC 2 cut(s) 227, 258
DpnII GATC 2 cut(s) 225, 256
Ecl136II GAGCTC 1 cut(s) 295
Eco130I CCWWGG 2 cut(s) 319, 472
Eco24I GRGCYC 1 cut(s) 297
Eco47I GGWCC 1 cut(s) 303
Eco53kI GAGCTC 1 cut(s) 295
EcoICRI GAGCTC 1 cut(s) 295
EcoT14I CCWWGG 2 cut(s) 319, 472
EcoT38I GRGCYC 1 cut(s) 297
ErhI CCWWGG 2 cut(s) 319, 472
FaeI CATG 3 cut(s) 254, 323, 430
FaiI YATR 3 cut(s) 252, 321, 428
FaqI GGGAC 1 cut(s) 316
FatI CATG 3 cut(s) 250, 319, 426
FblI GTMKAC 1 cut(s) 269
Fnu4HI GCNGC 3 cut(s) 181, 464, 467
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 3 cut(s) 181, 464, 467
GlaI GCGC 2 cut(s) 391, 470
GluI GCNGC 3 cut(s) 181, 464, 467
HaeII RGCGCY 1 cut(s) 472
HaeIII GGCC 1 cut(s) 318
HgaI GACGC 1 cut(s) 111
HhaI GCGC 2 cut(s) 392, 471
Hin1II CATG 3 cut(s) 254, 323, 430
Hin6I GCGC 2 cut(s) 390, 469
HinP1I GCGC 2 cut(s) 390, 469
HindIII AAGCTT 1 cut(s) 153
Hpy166II GTNNAC 2 cut(s) 270, 480
Hpy188I TCNGA 1 cut(s) 101
Hpy188III TCNNGA 1 cut(s) 229
Hpy8I GTNNAC 2 cut(s) 270, 480
HpyCH4V TGCA 2 cut(s) 349, 466
HpyF10VI GCNNNNNNNGC 2 cut(s) 180, 292
HpyF3I CTNAG 3 cut(s) 11, 219, 330
Hsp92II CATG 3 cut(s) 254, 323, 430
HspAI GCGC 2 cut(s) 390, 469
KpnI GGTACC 1 cut(s) 356
Kzo9I GATC 2 cut(s) 225, 256
LmnI GCTCC 3 cut(s) 167, 292, 423
Lsp1109I GCAGC 3 cut(s) 192, 450, 478
MalI GATC 2 cut(s) 227, 258
MboI GATC 2 cut(s) 225, 256
MboII GAAGA 1 cut(s) 394
MhlI GDGCHC 1 cut(s) 297
MluCI AATT 1 cut(s) 344
MmeI TCCRAC 1 cut(s) 270
MnlI CCTC 5 cut(s) 31, 100, 216, 308, 339
MseI TTAA 3 cut(s) 77, 309, 431
MwoI GCNNNNNNNGC 2 cut(s) 180, 292
NcoI CCATGG 1 cut(s) 319
NdeII GATC 2 cut(s) 225, 256
NlaIII CATG 3 cut(s) 254, 323, 430
NlaIV GGNNCC 3 cut(s) 304, 354, 446
NspI RCATGY 1 cut(s) 430
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PkrI GCNGC 3 cut(s) 182, 465, 468
Psp124BI GAGCTC 1 cut(s) 297
PspN4I GGNNCC 3 cut(s) 304, 354, 446
PspPI GGNCC 1 cut(s) 303
PstI CTGCAG 1 cut(s) 468
RsaI GTAC 4 cut(s) 212, 335, 354, 481
RsaNI GTAC 4 cut(s) 211, 334, 353, 480
SacI GAGCTC 1 cut(s) 297
SaqAI TTAA 3 cut(s) 77, 309, 431
SatI GCNGC 3 cut(s) 181, 464, 467
Sau3AI GATC 2 cut(s) 225, 256
Sau96I GGNCC 1 cut(s) 303
SduI GDGCHC 1 cut(s) 297
SetI ASST 9 cut(s) 51, 72, 77, 157, 297, 339, 354, 358, 450
SfcI CTRYAG 1 cut(s) 464
SinI GGWCC 1 cut(s) 303
Sse9I AATT 1 cut(s) 344
SstI GAGCTC 1 cut(s) 297
StyI CCWWGG 2 cut(s) 319, 472
TaqI TCGA 1 cut(s) 255
TasI AATT 1 cut(s) 344
TatI WGTACW 1 cut(s) 479
Tru1I TTAA 3 cut(s) 77, 309, 431
Tru9I TTAA 3 cut(s) 77, 309, 431
TscAI CASTG 1 cut(s) 304
TseI GCWGC 3 cut(s) 180, 463, 466
TspRI CASTG 1 cut(s) 304
VpaK11BI GGWCC 1 cut(s) 303
XceI RCATGY 1 cut(s) 430
XmiI GTMKAC 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.