FvH4_4g13030

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0010130
Physical Location & Seq
Reverse (-)
16666916 .. 16669339
2424 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g13030.t1

Sequence Viewer

Length: 738 bp
ATGGGATTAGAGGTCCAGATAGAGCCTGTTTTCGGCCAGCTCACGGTTGAGTGGGCTGTACTGGGCTCAACTGTGCCGCCGCGGCAGTTCTTGTTCCACCTTCATGCCCCGGATACTTTGCGCCTTGGAATTCATGTCACTGATTTCCATTCTAATACATGGGAGGCTGTACGGTCGGTTAGTCAGCTCGAGGACATGCAGGACAGCATTGGAGTAGGAGGCAGTTGGTCAGAGTTCATTGACTACCTTGTAAATTCCATAAAGTCTGAAGATACAAAGCTTGTTTTAGAGGGATGTTCAGACTCGGATGGTGCTGTGAATGCAAAGTTGGTTGCTCAGAAGTCTAAAGGAATGCCTAAAATTTCCATTGCTCTCACAAAGCTTGTGGGCTTGGCTGCTACTGAGGCTATTGCAAATTTCTCTCTTCGGCTATTCAAGGAGTTCAAAAGCATCAATGACTCTTTTGTAGAAGAACGTGAGCGGTCTATGCAATTATCCAAAGTGGTATCAGCAGAAAAGGAAAGAAATGAGAGCATCCAGAGCCAACTCGAGCAGTATACTAAGAGGCAAAAGATACAACGGATCGGTTCTTCAGACGTTAATGGTGCACAAAACTCTCCAGAAAAACAAACTGCTCGAGATACAAAATCAATTAAAGTTGTCAATCGGGTGGTGCCTGCATATCGCAGGGCAAAAGTACGTGGTGCTGTTCTGCAGGATATCGAAGATGAAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

246

Amino Acids

27.26

Weight (kDa)

6.14

Isoelectric Point (pI)

37.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 673
AccBSI CCGCTC 1 cut(s) 481
AccI GTMKAC 1 cut(s) 557
AccII CGCG 1 cut(s) 82
AciI CCGC 4 cut(s) 77, 80, 82, 481
AclWI GGATC 1 cut(s) 590
AcoI YGGCCR 1 cut(s) 34
AcsI RAATTY 4 cut(s) 129, 253, 360, 415
AcuI CTGAAG 2 cut(s) 288, 576
AfaI GTAC 3 cut(s) 60, 171, 699
AfiI CCNNNNNNNGG 2 cut(s) 32, 43
AgsI TTSAA 2 cut(s) 436, 445
AjuI GAANNNNNNNTTGG 2 cut(s) 311, 343
AluBI AGCT 4 cut(s) 40, 187, 280, 382
AluI AGCT 4 cut(s) 40, 187, 280, 382
Alw21I GWGCWC 1 cut(s) 610
Alw44I GTGCAC 1 cut(s) 606
AlwI GGATC 1 cut(s) 590
Ama87I CYCGRG 3 cut(s) 188, 548, 636
AoxI GGCC 1 cut(s) 34
ApaLI GTGCAC 1 cut(s) 606
ApeKI GCWGC 1 cut(s) 395
ApoI RAATTY 4 cut(s) 129, 253, 360, 415
AspLEI GCGC 1 cut(s) 123
AspS9I GGNCC 1 cut(s) 13
AsuC2I CCSGG 1 cut(s) 110
AvaI CYCGRG 3 cut(s) 188, 548, 636
AvaII GGWCC 1 cut(s) 13
BaeGI GKGCMC 1 cut(s) 610
BanI GGYRCC 1 cut(s) 673
BanII GRGCYC 1 cut(s) 68
Bbv12I GWGCWC 1 cut(s) 610
BbvI GCAGC 1 cut(s) 382
BccI CCATC 1 cut(s) 302
BciVI GTATCC 1 cut(s) 106
BcnI CCSGG 1 cut(s) 110
BfmI CTRYAG 1 cut(s) 713
BfuI GTATCC 1 cut(s) 106
BglI GCCNNNNNGGC 1 cut(s) 82
BisI GCNGC 4 cut(s) 77, 80, 83, 396
BlsI GCNGC 4 cut(s) 78, 81, 84, 397
Bme1390I CCNGG 1 cut(s) 110
Bme18I GGWCC 1 cut(s) 13
BmeT110I CYCGRG 3 cut(s) 188, 548, 636
BmgT120I GGNCC 1 cut(s) 13
BmiI GGNNCC 1 cut(s) 675
BmrFI CCNGG 1 cut(s) 110
BmrI ACTGGG 1 cut(s) 71
BmsI GCATC 2 cut(s) 459, 543
BmuI ACTGGG 1 cut(s) 71
BpmI CTGGAG 1 cut(s) 603
BpuMI CCSGG 1 cut(s) 110
BsaAI YACGTR 1 cut(s) 701
BsaJI CCNNGG 3 cut(s) 80, 108, 124
Bsc4I CCNNNNNNNGG 2 cut(s) 32, 43
Bse1I ACTGG 1 cut(s) 66
Bse3DI GCAATG 1 cut(s) 366
BseDI CCNNGG 3 cut(s) 80, 108, 124
BseGI GGATG 3 cut(s) 299, 313, 534
BseLI CCNNNNNNNGG 2 cut(s) 32, 43
BseMI GCAATG 1 cut(s) 366
BseMII CTCAG 2 cut(s) 350, 393
BseNI ACTGG 1 cut(s) 66
BseSI GKGCMC 1 cut(s) 610
BseXI GCAGC 1 cut(s) 382
Bsh1236I CGCG 1 cut(s) 82
Bsh1285I CGRYCG 1 cut(s) 176
BshFI GGCC 1 cut(s) 36
BshNI GGYRCC 1 cut(s) 673
BsiEI CGRYCG 1 cut(s) 176
BsiHKAI GWGCWC 1 cut(s) 610
BsiHKCI CYCGRG 3 cut(s) 188, 548, 636
BsiSI CCGG 1 cut(s) 110
BslI CCNNNNNNNGG 2 cut(s) 32, 43
BsmI GAATGC 2 cut(s) 325, 357
BsnI GGCC 1 cut(s) 36
BsoBI CYCGRG 3 cut(s) 188, 548, 636
Bsp1286I GDGCHC 2 cut(s) 68, 610
Bsp143I GATC 1 cut(s) 582
BspACI CCGC 4 cut(s) 77, 80, 82, 481
BspANI GGCC 1 cut(s) 36
BspCNI CTCAG 2 cut(s) 349, 394
BspFNI CGCG 1 cut(s) 82
BspLI GGNNCC 1 cut(s) 675
BspMAI CTGCAG 1 cut(s) 717
BspPI GGATC 1 cut(s) 590
BspT107I GGYRCC 1 cut(s) 673
BsrBI CCGCTC 1 cut(s) 481
BsrDI GCAATG 1 cut(s) 366
BsrI ACTGG 1 cut(s) 66
BssECI CCNNGG 3 cut(s) 80, 108, 124
BssMI GATC 1 cut(s) 582
BssNAI GTATAC 1 cut(s) 558
BssT1I CCWWGG 1 cut(s) 124
Bst1107I GTATAC 1 cut(s) 558
Bst4CI ACNGT 3 cut(s) 46, 73, 174
Bst6I CTCTTC 1 cut(s) 429
BstBAI YACGTR 1 cut(s) 701
BstC8I GCNNGC 2 cut(s) 38, 678
BstDEI CTNAG 4 cut(s) 336, 402, 561, 735
BstDSI CCRYGG 1 cut(s) 80
BstF5I GGATG 3 cut(s) 299, 313, 534
BstFNI CGCG 1 cut(s) 82
BstHHI GCGC 1 cut(s) 123
BstKTI GATC 1 cut(s) 585
BstMBI GATC 1 cut(s) 582
BstMCI CGRYCG 1 cut(s) 176
BstMWI GCNNNNNNNGC 5 cut(s) 82, 320, 404, 487, 540
BstNSI RCATGY 1 cut(s) 199
BstSCI CCNGG 1 cut(s) 108
BstSFI CTRYAG 1 cut(s) 713
BstSLI GKGCMC 1 cut(s) 610
BstUI CGCG 1 cut(s) 82
BstV1I GCAGC 1 cut(s) 382
BstZ17I GTATAC 1 cut(s) 558
BsuI GTATCC 1 cut(s) 106
BsuRI GGCC 1 cut(s) 36
BtgI CCRYGG 1 cut(s) 80
BtsCI GGATG 3 cut(s) 299, 313, 534
BtsIMutI CAGTG 1 cut(s) 138
Cac8I GCNNGC 2 cut(s) 38, 678
CfoI GCGC 1 cut(s) 123
Cfr13I GGNCC 1 cut(s) 13
Cfr42I CCGCGG 1 cut(s) 83
Csp6I GTAC 3 cut(s) 59, 170, 698
CspCI CAANNNNNGTGG 2 cut(s) 366, 401
CviAII CATG 4 cut(s) 104, 134, 159, 196
CviQI GTAC 3 cut(s) 59, 170, 698
DdeI CTNAG 4 cut(s) 336, 402, 561, 735
DpnI GATC 1 cut(s) 584
DpnII GATC 1 cut(s) 582
EaeI YGGCCR 1 cut(s) 34
Eam1104I CTCTTC 1 cut(s) 429
EarI CTCTTC 1 cut(s) 429
Eco130I CCWWGG 1 cut(s) 124
Eco24I GRGCYC 1 cut(s) 68
Eco32I GATATC 1 cut(s) 721
Eco47I GGWCC 1 cut(s) 13
Eco57I CTGAAG 2 cut(s) 288, 576
Eco88I CYCGRG 3 cut(s) 188, 548, 636
EcoRI GAATTC 1 cut(s) 129
EcoRV GATATC 1 cut(s) 721
EcoT14I CCWWGG 1 cut(s) 124
EcoT38I GRGCYC 1 cut(s) 68
ErhI CCWWGG 1 cut(s) 124
FaeI CATG 4 cut(s) 107, 137, 162, 199
FaiI YATR 8 cut(s) 105, 135, 160, 197, 260, 488, 558, 682
FatI CATG 4 cut(s) 103, 133, 158, 195
FblI GTMKAC 1 cut(s) 557
Fnu4HI GCNGC 4 cut(s) 77, 80, 83, 396
FokI GGATG 3 cut(s) 306, 320, 521
FriOI GRGCYC 1 cut(s) 68
Fsp4HI GCNGC 4 cut(s) 77, 80, 83, 396
GlaI GCGC 1 cut(s) 122
GluI GCNGC 4 cut(s) 77, 80, 83, 396
GsuI CTGGAG 1 cut(s) 603
HaeIII GGCC 1 cut(s) 36
HapII CCGG 1 cut(s) 110
HhaI GCGC 1 cut(s) 123
Hin1II CATG 4 cut(s) 107, 137, 162, 199
Hin6I GCGC 1 cut(s) 121
HinP1I GCGC 1 cut(s) 121
HindIII AAGCTT 2 cut(s) 278, 380
HinfI GANTC 2 cut(s) 302, 458
HpaII CCGG 1 cut(s) 110
Hpy166II GTNNAC 2 cut(s) 558, 608
Hpy188I TCNGA 6 cut(s) 232, 268, 301, 307, 339, 595
Hpy188III TCNNGA 4 cut(s) 16, 538, 620, 638
Hpy8I GTNNAC 2 cut(s) 558, 608
HpyAV CCTTC 1 cut(s) 110
HpyCH4III ACNGT 3 cut(s) 46, 73, 174
HpyCH4IV ACGT 3 cut(s) 475, 597, 700
HpyCH4V TGCA 7 cut(s) 199, 323, 413, 490, 608, 680, 715
HpyF10VI GCNNNNNNNGC 5 cut(s) 82, 320, 404, 487, 540
HpyF3I CTNAG 4 cut(s) 336, 402, 561, 735
HpySE526I ACGT 3 cut(s) 475, 597, 700
Hsp92II CATG 4 cut(s) 107, 137, 162, 199
HspAI GCGC 1 cut(s) 121
KspI CCGCGG 1 cut(s) 83
Kzo9I GATC 1 cut(s) 582
Lsp1109I GCAGC 1 cut(s) 382
LweI GCATC 2 cut(s) 459, 543
MaeII ACGT 3 cut(s) 475, 597, 700
MaeIII GTNAC 1 cut(s) 136
MalI GATC 1 cut(s) 584
MbiI CCGCTC 1 cut(s) 481
MboI GATC 1 cut(s) 582
MboII GAAGA 5 cut(s) 281, 416, 482, 582, 737
MhlI GDGCHC 2 cut(s) 68, 610
MluCI AATT 6 cut(s) 129, 253, 360, 415, 491, 651
MlyI GAGTC 2 cut(s) 296, 452
MnlI CCTC 7 cut(s) 4, 157, 184, 212, 283, 397, 558
MseI TTAA 2 cut(s) 600, 654
MslI CAYNNNNRTG 1 cut(s) 102
MspA1I CMGCKG 1 cut(s) 82
MspI CCGG 1 cut(s) 110
MspR9I CCNGG 1 cut(s) 110
Mva1269I GAATGC 2 cut(s) 325, 357
MvnI CGCG 1 cut(s) 82
MwoI GCNNNNNNNGC 5 cut(s) 82, 320, 404, 487, 540
NciI CCSGG 1 cut(s) 110
NdeII GATC 1 cut(s) 582
NlaIII CATG 4 cut(s) 107, 137, 162, 199
NlaIV GGNNCC 1 cut(s) 675
NmuCI GTSAC 1 cut(s) 136
NspI RCATGY 1 cut(s) 199
PaeR7I CTCGAG 3 cut(s) 188, 548, 636
PctI GAATGC 2 cut(s) 325, 357
PkrI GCNGC 4 cut(s) 78, 81, 84, 397
PleI GAGTC 2 cut(s) 296, 452
PpsI GAGTC 2 cut(s) 296, 452
Ppu21I YACGTR 1 cut(s) 701
PspN4I GGNNCC 1 cut(s) 675
PspPI GGNCC 1 cut(s) 13
PspXI VCTCGAGB 2 cut(s) 188, 548
PstI CTGCAG 1 cut(s) 717
RsaI GTAC 3 cut(s) 60, 171, 699
RsaNI GTAC 3 cut(s) 59, 170, 698
RseI CAYNNNNRTG 1 cut(s) 102
SacII CCGCGG 1 cut(s) 83
SaqAI TTAA 2 cut(s) 600, 654
SatI GCNGC 4 cut(s) 77, 80, 83, 396
Sau3AI GATC 1 cut(s) 582
Sau96I GGNCC 1 cut(s) 13
SchI GAGTC 2 cut(s) 296, 452
ScrFI CCNGG 1 cut(s) 110
SduI GDGCHC 2 cut(s) 68, 610
SfaNI GCATC 2 cut(s) 459, 543
SfcI CTRYAG 1 cut(s) 713
Sfr274I CTCGAG 3 cut(s) 188, 548, 636
Sfr303I CCGCGG 1 cut(s) 83
SgrBI CCGCGG 1 cut(s) 83
SinI GGWCC 1 cut(s) 13
SlaI CTCGAG 3 cut(s) 188, 548, 636
SmiMI CAYNNNNRTG 1 cut(s) 102
SmlI CTYRAG 3 cut(s) 188, 548, 636
SmoI CTYRAG 3 cut(s) 188, 548, 636
Sse9I AATT 6 cut(s) 129, 253, 360, 415, 491, 651
SsiI CCGC 4 cut(s) 77, 80, 82, 481
StyD4I CCNGG 1 cut(s) 108
StyI CCWWGG 1 cut(s) 124
TaaI ACNGT 3 cut(s) 46, 73, 174
TaiI ACGT 3 cut(s) 478, 600, 703
TaqI TCGA 4 cut(s) 189, 549, 637, 723
TasI AATT 6 cut(s) 129, 253, 360, 415, 491, 651
TatI WGTACW 1 cut(s) 58
TauI GCSGC 3 cut(s) 79, 82, 85
Tru1I TTAA 2 cut(s) 600, 654
Tru9I TTAA 2 cut(s) 600, 654
TscAI CASTG 1 cut(s) 145
TseFI GTSAC 1 cut(s) 136
TseI GCWGC 1 cut(s) 395
Tsp45I GTSAC 1 cut(s) 136
TspDTI ATGAA 3 cut(s) 92, 122, 226
TspGWI ACGGA 1 cut(s) 595
TspRI CASTG 1 cut(s) 145
VneI GTGCAC 1 cut(s) 606
VpaK11BI GGWCC 1 cut(s) 13
XapI RAATTY 4 cut(s) 129, 253, 360, 415
XceI RCATGY 1 cut(s) 199
XhoI CTCGAG 3 cut(s) 188, 548, 636
XmiI GTMKAC 1 cut(s) 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.