RLG00000008263

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0010130
Physical Location & Seq
Forward (+)
27943935 .. 27945190
1256 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008263

Sequence Viewer

Length: 396 bp
ATGGGATTAGAGGTCCAGTTGGAGCCGGCTTTCGGCCAACTCTCGGTTGAATGGGCGGTTACGGGATCAACTCCGCCGCAACATCAATTCTTGTTCCACCTCCATGCCCAGGATTCTTTGCACCTGGGAACATGGCCCCCACTCGGTCCTTACAGCGTCCGCCATTGCCTGGGAATCCATGTCACTGATTTCCACTCTAATACATGGGAGGCTGTACGGTCGGTTAGTCAGCTGGATGATATGCAGGACAGCATTGGAATAGGAGGCGCTTGGTCGGAGTTCATAGACTATCTTGTAAATTCCATAAAATCTGAAGATACAAAGCTTGTTTTGGAGGGACCGTTAAACTCAGATGGTGCTGCAGCTGCAAAGTTAATTAGTTGCTCAGAAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

132

Amino Acids

14.28

Weight (kDa)

4.71

Isoelectric Point (pI)

25.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 169
AciI CCGC 4 cut(s) 56, 74, 77, 160
AclWI GGATC 1 cut(s) 73
AcoI YGGCCR 1 cut(s) 34
AcsI RAATTY 1 cut(s) 298
AcuI CTGAAG 1 cut(s) 333
AfaI GTAC 1 cut(s) 216
AfiI CCNNNNNNNGG 5 cut(s) 32, 43, 109, 143, 169
AgsI TTSAA 1 cut(s) 50
AjnI CCWGG 3 cut(s) 108, 123, 168
AluBI AGCT 3 cut(s) 232, 325, 365
AluI AGCT 3 cut(s) 232, 325, 365
AlwI GGATC 1 cut(s) 73
AoxI GGCC 2 cut(s) 34, 134
ApeKI GCWGC 3 cut(s) 359, 362, 365
ApoI RAATTY 1 cut(s) 298
AspLEI GCGC 1 cut(s) 269
AspS9I GGNCC 4 cut(s) 13, 135, 146, 338
AvaII GGWCC 3 cut(s) 13, 146, 338
BbvI GCAGC 3 cut(s) 346, 352, 374
BccI CCATC 1 cut(s) 347
BciT130I CCWGG 3 cut(s) 110, 125, 170
BfmI CTRYAG 1 cut(s) 360
BfoI RGCGCY 1 cut(s) 270
BisI GCNGC 4 cut(s) 77, 360, 363, 366
BlsI GCNGC 4 cut(s) 78, 361, 364, 367
Bme1390I CCNGG 3 cut(s) 110, 125, 170
Bme18I GGWCC 3 cut(s) 13, 146, 338
BmgT120I GGNCC 4 cut(s) 13, 135, 146, 338
BmiI GGNNCC 3 cut(s) 24, 137, 339
BmrFI CCNGG 3 cut(s) 110, 125, 170
BsaJI CCNNGG 3 cut(s) 108, 124, 169
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bsc4I CCNNNNNNNGG 5 cut(s) 32, 43, 109, 143, 169
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 1 cut(s) 16
Bse3DI GCAATG 1 cut(s) 163
BseBI CCWGG 3 cut(s) 110, 125, 170
BseDI CCNNGG 3 cut(s) 108, 124, 169
BseGI GGATG 1 cut(s) 241
BseLI CCNNNNNNNGG 5 cut(s) 32, 43, 109, 143, 169
BseMI GCAATG 1 cut(s) 163
BseMII CTCAG 1 cut(s) 363
BseNI ACTGG 1 cut(s) 16
BseXI GCAGC 3 cut(s) 346, 352, 374
Bsh1285I CGRYCG 1 cut(s) 221
BshFI GGCC 2 cut(s) 36, 136
BsiEI CGRYCG 1 cut(s) 221
BsiSI CCGG 1 cut(s) 26
BslFI GGGAC 1 cut(s) 351
BslI CCNNNNNNNGG 5 cut(s) 32, 43, 109, 143, 169
BsmFI GGGAC 1 cut(s) 351
BsnI GGCC 2 cut(s) 36, 136
Bsp143I GATC 1 cut(s) 65
BspACI CCGC 4 cut(s) 56, 74, 77, 160
BspANI GGCC 2 cut(s) 36, 136
BspCNI CTCAG 1 cut(s) 362
BspLI GGNNCC 3 cut(s) 24, 137, 339
BspMAI CTGCAG 1 cut(s) 364
BspPI GGATC 1 cut(s) 73
BsrDI GCAATG 1 cut(s) 163
BsrFI RCCGGY 1 cut(s) 25
BsrI ACTGG 1 cut(s) 16
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 3 cut(s) 108, 124, 169
BssMI GATC 1 cut(s) 65
Bst2UI CCWGG 3 cut(s) 110, 125, 170
Bst4CI ACNGT 2 cut(s) 219, 342
BstC8I GCNNGC 1 cut(s) 27
BstDEI CTNAG 2 cut(s) 349, 385
BstF5I GGATG 1 cut(s) 241
BstH2I RGCGCY 1 cut(s) 270
BstHHI GCGC 1 cut(s) 269
BstKTI GATC 1 cut(s) 68
BstMBI GATC 1 cut(s) 65
BstMCI CGRYCG 1 cut(s) 221
BstMWI GCNNNNNNNGC 1 cut(s) 365
BstNI CCWGG 3 cut(s) 110, 125, 170
BstSCI CCNGG 3 cut(s) 108, 123, 168
BstSFI CTRYAG 1 cut(s) 360
BstV1I GCAGC 3 cut(s) 346, 352, 374
BsuRI GGCC 2 cut(s) 36, 136
BtsCI GGATG 1 cut(s) 241
BtsIMutI CAGTG 1 cut(s) 183
Cac8I GCNNGC 1 cut(s) 27
CfoI GCGC 1 cut(s) 269
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 4 cut(s) 13, 135, 146, 338
CseI GACGC 1 cut(s) 145
Csp6I GTAC 1 cut(s) 215
CviAII CATG 4 cut(s) 104, 132, 179, 204
CviJI RGCY 8 cut(s) 25, 29, 36, 136, 212, 232, 325, 365
CviKI_1 RGCY 8 cut(s) 25, 29, 36, 136, 212, 232, 325, 365
CviQI GTAC 1 cut(s) 215
DdeI CTNAG 2 cut(s) 349, 385
DpnI GATC 1 cut(s) 67
DpnII GATC 1 cut(s) 65
EaeI YGGCCR 1 cut(s) 34
EciI GGCGGA 2 cut(s) 63, 149
Eco47I GGWCC 3 cut(s) 13, 146, 338
Eco57I CTGAAG 1 cut(s) 333
EcoRII CCWGG 3 cut(s) 108, 123, 168
FaeI CATG 4 cut(s) 107, 135, 182, 207
FaiI YATR 7 cut(s) 105, 133, 180, 205, 242, 284, 305
FaqI GGGAC 1 cut(s) 351
FatI CATG 4 cut(s) 103, 131, 178, 203
Fnu4HI GCNGC 4 cut(s) 77, 360, 363, 366
FokI GGATG 1 cut(s) 248
Fsp4HI GCNGC 4 cut(s) 77, 360, 363, 366
GlaI GCGC 1 cut(s) 268
GluI GCNGC 4 cut(s) 77, 360, 363, 366
HaeII RGCGCY 1 cut(s) 270
HaeIII GGCC 2 cut(s) 36, 136
HapII CCGG 1 cut(s) 26
HgaI GACGC 1 cut(s) 145
HhaI GCGC 1 cut(s) 269
Hin1II CATG 4 cut(s) 107, 135, 182, 207
Hin6I GCGC 1 cut(s) 267
HinP1I GCGC 1 cut(s) 267
HindIII AAGCTT 1 cut(s) 323
HinfI GANTC 2 cut(s) 113, 174
HpaII CCGG 1 cut(s) 26
Hpy188I TCNGA 4 cut(s) 277, 313, 352, 388
HpyCH4III ACNGT 2 cut(s) 219, 342
HpyCH4V TGCA 4 cut(s) 121, 244, 362, 368
HpyF10VI GCNNNNNNNGC 1 cut(s) 365
HpyF3I CTNAG 2 cut(s) 349, 385
Hsp92II CATG 4 cut(s) 107, 135, 182, 207
HspAI GCGC 1 cut(s) 267
KroI GCCGGC 1 cut(s) 25
KroNI GCCGGC 1 cut(s) 27
Kzo9I GATC 1 cut(s) 65
LmnI GCTCC 1 cut(s) 22
Lsp1109I GCAGC 3 cut(s) 346, 352, 374
MaeIII GTNAC 2 cut(s) 58, 181
MalI GATC 1 cut(s) 67
MboI GATC 1 cut(s) 65
MboII GAAGA 1 cut(s) 326
MluCI AATT 3 cut(s) 86, 298, 375
MmeI TCCRAC 1 cut(s) 255
MnlI CCTC 5 cut(s) 4, 110, 202, 257, 328
MroNI GCCGGC 1 cut(s) 25
MseI TTAA 2 cut(s) 344, 374
MslI CAYNNNNRTG 1 cut(s) 102
MspA1I CMGCKG 2 cut(s) 232, 365
MspI CCGG 1 cut(s) 26
MspR9I CCNGG 3 cut(s) 110, 125, 170
MvaI CCWGG 3 cut(s) 110, 125, 170
MwoI GCNNNNNNNGC 1 cut(s) 365
NaeI GCCGGC 1 cut(s) 27
NdeII GATC 1 cut(s) 65
NgoMIV GCCGGC 1 cut(s) 25
NlaIII CATG 4 cut(s) 107, 135, 182, 207
NlaIV GGNNCC 3 cut(s) 24, 137, 339
NmuCI GTSAC 1 cut(s) 181
PdiI GCCGGC 1 cut(s) 27
PfeI GAWTC 2 cut(s) 113, 174
PflMI CCANNNNNTGG 1 cut(s) 169
PkrI GCNGC 4 cut(s) 78, 361, 364, 367
Psp6I CCWGG 3 cut(s) 108, 123, 168
PspGI CCWGG 3 cut(s) 108, 123, 168
PspN4I GGNNCC 3 cut(s) 24, 137, 339
PspPI GGNCC 4 cut(s) 13, 135, 146, 338
PstI CTGCAG 1 cut(s) 364
PvuII CAGCTG 2 cut(s) 232, 365
RsaI GTAC 1 cut(s) 216
RsaNI GTAC 1 cut(s) 215
RseI CAYNNNNRTG 1 cut(s) 102
SaqAI TTAA 2 cut(s) 344, 374
SatI GCNGC 4 cut(s) 77, 360, 363, 366
Sau3AI GATC 1 cut(s) 65
Sau96I GGNCC 4 cut(s) 13, 135, 146, 338
ScrFI CCNGG 3 cut(s) 110, 125, 170
SetI ASST 6 cut(s) 15, 102, 126, 234, 327, 367
SfcI CTRYAG 1 cut(s) 360
SinI GGWCC 3 cut(s) 13, 146, 338
SmiMI CAYNNNNRTG 1 cut(s) 102
Sse9I AATT 3 cut(s) 86, 298, 375
SsiI CCGC 4 cut(s) 56, 74, 77, 160
StyD4I CCNGG 3 cut(s) 108, 123, 168
TaaI ACNGT 2 cut(s) 219, 342
TaqII GACCGA 1 cut(s) 134
TasI AATT 3 cut(s) 86, 298, 375
TauI GCSGC 1 cut(s) 79
TfiI GAWTC 2 cut(s) 113, 174
Tru1I TTAA 2 cut(s) 344, 374
Tru9I TTAA 2 cut(s) 344, 374
TscAI CASTG 1 cut(s) 190
TseFI GTSAC 1 cut(s) 181
TseI GCWGC 3 cut(s) 359, 362, 365
Tsp45I GTSAC 1 cut(s) 181
TspDTI ATGAA 1 cut(s) 271
TspRI CASTG 1 cut(s) 190
Van91I CCANNNNNTGG 1 cut(s) 169
VpaK11BI GGWCC 3 cut(s) 13, 146, 338
XapI RAATTY 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.