MD17G1201700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0010130
Physical Location & Seq
Forward (+)
24373668 .. 24378738
5071 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1201700.v1.1.491

Sequence Viewer

Length: 753 bp
ATGGAGGGGTTAGAAGTAGATTTGAAGCCCATATTCGGGCAGCCCAGGGTGGAGGGGCCAGCTAATGCTTTGACTCCGCCGCGGCAGTTCCTGTTCCACGTCCATGGCTCCACCGCTGGTCCTGCGCACCTCAGAATCCATGTCACCGATTTCCACTCCAATCACTGGGAGGCCGTGCGGTCCGTCTCCCAGCTCGACGACCTGAGGGACAGCATTGGCATAGGAGGGTCTTGGTCAGAGTTCATAGACTATCTTGTTGCTTCCCTAAAGTCTCAAGACGTAAAGCTTGCTTTCGATGATGGTTGTGCACATGCAAAATTAATTGCTCAAAAATCAAAAGGAATGCCTCGAATTTCCATTTCTCTCACAAAACTTGTGGATACTGCTGCTAGTGAGGCTATTGCAAATCTATCTCTTCAGCTTTTTGGAGAATTTAAAAACCTCCATGAGTCTTACGTGGAAGAACAACAGTGCTCTGTTGAATTGTCAACATTGATATCAGCGGAAAAGGAAAAAAATGCTAGTATTCAGAGCCAACTGGAACAGTATACAAGAAGGCAAAAGTTACAAAGGATTAATTCCTCAGATAGAGCAGATGCTTCTGGACTATTCAGTAATGGTGTAAATAATTCTCCAGAGAAACGAGCGGGTCGTGATACCAACTCAGCAACTGCCAATCGGGTTGTGCCTGCATATCGTAGGGCAAAAGTAAGAGGTGCTGTTCTGCAGGATACAGAAGATGAACACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

251

Amino Acids

27.59

Weight (kDa)

6.51

Isoelectric Point (pI)

35.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 126
AccB7I CCANNNNNTGG 1 cut(s) 165
AccBSI CCGCTC 1 cut(s) 647
AccI GTMKAC 1 cut(s) 548
AccII CGCG 1 cut(s) 82
AciI CCGC 7 cut(s) 77, 80, 82, 114, 178, 503, 647
AcsI RAATTY 2 cut(s) 351, 431
AcuI CTGAAG 1 cut(s) 401
AfiI CCNNNNNNNGG 3 cut(s) 35, 36, 165
AgsI TTSAA 2 cut(s) 25, 482
AjiI CACGTC 1 cut(s) 100
AjnI CCWGG 1 cut(s) 44
AluBI AGCT 4 cut(s) 62, 193, 286, 421
AluI AGCT 4 cut(s) 62, 193, 286, 421
Alw21I GWGCWC 2 cut(s) 310, 476
Alw26I GTCTC 2 cut(s) 190, 276
Alw44I GTGCAC 1 cut(s) 306
AlwNI CAGNNNCTG 2 cut(s) 91, 671
AoxI GGCC 2 cut(s) 56, 171
ApaLI GTGCAC 1 cut(s) 306
ApeKI GCWGC 2 cut(s) 40, 386
ApoI RAATTY 2 cut(s) 351, 431
AseI ATTAAT 2 cut(s) 320, 576
AspLEI GCGC 1 cut(s) 127
AspS9I GGNCC 3 cut(s) 56, 119, 180
AsuHPI GGTGA 1 cut(s) 136
AvaII GGWCC 2 cut(s) 119, 180
AxyI CCTNAGG 1 cut(s) 203
BaeGI GKGCMC 1 cut(s) 310
Bbv12I GWGCWC 2 cut(s) 310, 476
BbvI GCAGC 2 cut(s) 52, 373
BccI CCATC 1 cut(s) 293
BceAI ACGGC 1 cut(s) 158
BciT130I CCWGG 1 cut(s) 46
BciVI GTATCC 2 cut(s) 373, 724
BcoDI GTCTC 2 cut(s) 190, 276
BfaI CTAG 2 cut(s) 390, 522
BfmI CTRYAG 1 cut(s) 725
BfuI GTATCC 2 cut(s) 373, 724
BisI GCNGC 4 cut(s) 41, 80, 83, 387
BlsI GCNGC 4 cut(s) 42, 81, 84, 388
Bme1390I CCNGG 1 cut(s) 46
Bme18I GGWCC 2 cut(s) 119, 180
BmgBI CACGTC 1 cut(s) 100
BmgT120I GGNCC 3 cut(s) 56, 119, 180
BmiI GGNNCC 2 cut(s) 57, 109
BmrFI CCNGG 1 cut(s) 46
BmrI ACTGGG 1 cut(s) 175
BmsI GCATC 1 cut(s) 586
BmuI ACTGGG 1 cut(s) 175
BpmI CTGGAG 1 cut(s) 618
BpuEI CTTGAG 1 cut(s) 258
BsaAI YACGTR 1 cut(s) 457
BsaJI CCNNGG 4 cut(s) 44, 45, 80, 103
Bsc4I CCNNNNNNNGG 3 cut(s) 35, 36, 165
Bse1I ACTGG 2 cut(s) 170, 543
Bse21I CCTNAGG 1 cut(s) 203
BseBI CCWGG 1 cut(s) 46
BseDI CCNNGG 4 cut(s) 44, 45, 80, 103
BseLI CCNNNNNNNGG 3 cut(s) 35, 36, 165
BseMII CTCAG 4 cut(s) 145, 194, 597, 678
BseNI ACTGG 2 cut(s) 170, 543
BseSI GKGCMC 1 cut(s) 310
BseXI GCAGC 2 cut(s) 52, 373
BseYI CCCAGC 1 cut(s) 189
Bsh1236I CGCG 1 cut(s) 82
BshFI GGCC 2 cut(s) 58, 173
BsiHKAI GWGCWC 2 cut(s) 310, 476
BslFI GGGAC 1 cut(s) 221
BslI CCNNNNNNNGG 3 cut(s) 35, 36, 165
BsmAI GTCTC 2 cut(s) 190, 276
BsmBI CGTCTC 1 cut(s) 190
BsmFI GGGAC 1 cut(s) 221
BsmI GAATGC 1 cut(s) 348
BsnI GGCC 2 cut(s) 58, 173
Bsp1286I GDGCHC 2 cut(s) 310, 476
Bsp19I CCATGG 1 cut(s) 103
BspACI CCGC 7 cut(s) 77, 80, 82, 114, 178, 503, 647
BspANI GGCC 2 cut(s) 58, 173
BspCNI CTCAG 4 cut(s) 144, 195, 596, 677
BspFNI CGCG 1 cut(s) 82
BspLI GGNNCC 2 cut(s) 57, 109
BspMAI CTGCAG 1 cut(s) 729
BsrBI CCGCTC 1 cut(s) 647
BsrI ACTGG 2 cut(s) 170, 543
BssECI CCNNGG 4 cut(s) 44, 45, 80, 103
BssNAI GTATAC 1 cut(s) 549
BssT1I CCWWGG 1 cut(s) 103
Bst1107I GTATAC 1 cut(s) 549
Bst2UI CCWGG 1 cut(s) 46
Bst4CI ACNGT 2 cut(s) 471, 546
Bst6I CTCTTC 1 cut(s) 420
BstBAI YACGTR 1 cut(s) 457
BstC8I GCNNGC 3 cut(s) 60, 288, 690
BstDEI CTNAG 4 cut(s) 131, 203, 583, 664
BstDSI CCRYGG 2 cut(s) 80, 103
BstFNI CGCG 1 cut(s) 82
BstHHI GCGC 1 cut(s) 127
BstMAI GTCTC 2 cut(s) 190, 276
BstMWI GCNNNNNNNGC 2 cut(s) 122, 395
BstNI CCWGG 1 cut(s) 46
BstNSI RCATGY 1 cut(s) 314
BstSCI CCNGG 1 cut(s) 44
BstSFI CTRYAG 1 cut(s) 725
BstSLI GKGCMC 1 cut(s) 310
BstUI CGCG 1 cut(s) 82
BstV1I GCAGC 2 cut(s) 52, 373
BstXI CCANNNNNNTGG 1 cut(s) 104
BstZ17I GTATAC 1 cut(s) 549
Bsu36I CCTNAGG 1 cut(s) 203
BsuI GTATCC 2 cut(s) 373, 724
BsuRI GGCC 2 cut(s) 58, 173
BtgI CCRYGG 2 cut(s) 80, 103
BtrI CACGTC 1 cut(s) 100
BtsIMutI CAGTG 2 cut(s) 163, 476
Cac8I GCNNGC 3 cut(s) 60, 288, 690
CaiI CAGNNNCTG 2 cut(s) 91, 671
CfoI GCGC 1 cut(s) 127
Cfr13I GGNCC 3 cut(s) 56, 119, 180
Cfr42I CCGCGG 1 cut(s) 83
CpoI CGGWCCG 1 cut(s) 180
CspCI CAANNNNNGTGG 2 cut(s) 357, 392
CspI CGGWCCG 1 cut(s) 180
CviAII CATG 4 cut(s) 104, 140, 311, 446
DdeI CTNAG 4 cut(s) 131, 203, 583, 664
DraI TTTAAA 1 cut(s) 436
Eam1104I CTCTTC 1 cut(s) 420
EarI CTCTTC 1 cut(s) 420
EciI GGCGGA 1 cut(s) 66
Eco130I CCWWGG 1 cut(s) 103
Eco32I GATATC 1 cut(s) 498
Eco47I GGWCC 2 cut(s) 119, 180
Eco57I CTGAAG 1 cut(s) 401
Eco81I CCTNAGG 1 cut(s) 203
EcoRII CCWGG 1 cut(s) 44
EcoRV GATATC 1 cut(s) 498
EcoT14I CCWWGG 1 cut(s) 103
ErhI CCWWGG 1 cut(s) 103
Esp3I CGTCTC 1 cut(s) 190
FaeI CATG 4 cut(s) 107, 143, 314, 449
FaiI YATR 9 cut(s) 32, 105, 141, 221, 245, 312, 447, 549, 694
FaqI GGGAC 1 cut(s) 221
FatI CATG 4 cut(s) 103, 139, 310, 445
FauI CCCGC 1 cut(s) 640
FblI GTMKAC 1 cut(s) 548
Fnu4HI GCNGC 4 cut(s) 41, 80, 83, 387
Fsp4HI GCNGC 4 cut(s) 41, 80, 83, 387
FspBI CTAG 2 cut(s) 390, 522
FspI TGCGCA 1 cut(s) 126
GlaI GCGC 1 cut(s) 126
GluI GCNGC 4 cut(s) 41, 80, 83, 387
GsaI CCCAGC 1 cut(s) 193
GsuI CTGGAG 1 cut(s) 618
HaeIII GGCC 2 cut(s) 58, 173
HhaI GCGC 1 cut(s) 127
Hin1II CATG 4 cut(s) 107, 143, 314, 449
Hin6I GCGC 1 cut(s) 125
HinP1I GCGC 1 cut(s) 125
HincII GTYRAC 1 cut(s) 489
HindII GTYRAC 1 cut(s) 489
HindIII AAGCTT 1 cut(s) 284
HinfI GANTC 3 cut(s) 73, 135, 449
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 3 cut(s) 308, 489, 549
Hpy188I TCNGA 4 cut(s) 134, 238, 531, 586
Hpy188III TCNNGA 4 cut(s) 275, 603, 635, 653
Hpy8I GTNNAC 3 cut(s) 308, 489, 549
Hpy99I CGWCG 1 cut(s) 200
HpyAV CCTTC 1 cut(s) 549
HpyCH4III ACNGT 2 cut(s) 471, 546
HpyCH4IV ACGT 3 cut(s) 99, 279, 456
HpyCH4V TGCA 5 cut(s) 308, 314, 404, 692, 727
HpyF10VI GCNNNNNNNGC 2 cut(s) 122, 395
HpyF3I CTNAG 4 cut(s) 131, 203, 583, 664
HpySE526I ACGT 3 cut(s) 99, 279, 456
Hsp92II CATG 4 cut(s) 107, 143, 314, 449
HspAI GCGC 1 cut(s) 125
KspI CCGCGG 1 cut(s) 83
LmnI GCTCC 1 cut(s) 113
Lsp1109I GCAGC 2 cut(s) 52, 373
LweI GCATC 1 cut(s) 586
MaeI CTAG 2 cut(s) 390, 522
MaeII ACGT 3 cut(s) 99, 279, 456
MaeIII GTNAC 2 cut(s) 142, 564
MbiI CCGCTC 1 cut(s) 647
MboII GAAGA 3 cut(s) 407, 473, 749
MhlI GDGCHC 2 cut(s) 310, 476
MluCI AATT 7 cut(s) 317, 321, 351, 431, 482, 577, 628
MlyI GAGTC 2 cut(s) 67, 458
MseI TTAA 3 cut(s) 320, 435, 576
MslI CAYNNNNRTG 1 cut(s) 102
MspA1I CMGCKG 3 cut(s) 82, 116, 503
MspR9I CCNGG 1 cut(s) 46
Mva1269I GAATGC 1 cut(s) 348
MvaI CCWGG 1 cut(s) 46
MvnI CGCG 1 cut(s) 82
MwoI GCNNNNNNNGC 2 cut(s) 122, 395
NcoI CCATGG 1 cut(s) 103
NlaIII CATG 4 cut(s) 107, 143, 314, 449
NlaIV GGNNCC 2 cut(s) 57, 109
NmuCI GTSAC 1 cut(s) 142
NsbI TGCGCA 1 cut(s) 126
NspI RCATGY 1 cut(s) 314
PasI CCCWGGG 1 cut(s) 45
PcsI WCGNNNNNNNCGW 1 cut(s) 649
PctI GAATGC 1 cut(s) 348
PfeI GAWTC 1 cut(s) 135
PflMI CCANNNNNTGG 1 cut(s) 165
PkrI GCNGC 4 cut(s) 42, 81, 84, 388
PleI GAGTC 2 cut(s) 67, 457
PpsI GAGTC 2 cut(s) 67, 457
Ppu21I YACGTR 1 cut(s) 457
PshBI ATTAAT 2 cut(s) 320, 576
Psp6I CCWGG 1 cut(s) 44
PspFI CCCAGC 1 cut(s) 189
PspGI CCWGG 1 cut(s) 44
PspN4I GGNNCC 2 cut(s) 57, 109
PspPI GGNCC 3 cut(s) 56, 119, 180
PstI CTGCAG 1 cut(s) 729
PstNI CAGNNNCTG 2 cut(s) 91, 671
RseI CAYNNNNRTG 1 cut(s) 102
Rsr2I CGGWCCG 1 cut(s) 180
RsrII CGGWCCG 1 cut(s) 180
SacII CCGCGG 1 cut(s) 83
SaqAI TTAA 3 cut(s) 320, 435, 576
SatI GCNGC 4 cut(s) 41, 80, 83, 387
Sau96I GGNCC 3 cut(s) 56, 119, 180
SchI GAGTC 2 cut(s) 67, 458
ScrFI CCNGG 1 cut(s) 46
SduI GDGCHC 2 cut(s) 310, 476
SfaNI GCATC 1 cut(s) 586
SfcI CTRYAG 1 cut(s) 725
Sfr303I CCGCGG 1 cut(s) 83
SgrBI CCGCGG 1 cut(s) 83
SinI GGWCC 2 cut(s) 119, 180
SmiMI CAYNNNNRTG 1 cut(s) 102
SmlI CTYRAG 1 cut(s) 273
SmoI CTYRAG 1 cut(s) 273
Sse9I AATT 7 cut(s) 317, 321, 351, 431, 482, 577, 628
SsiI CCGC 7 cut(s) 77, 80, 82, 114, 178, 503, 647
SspMI CTAG 2 cut(s) 390, 522
StyD4I CCNGG 1 cut(s) 44
StyI CCWWGG 1 cut(s) 103
TaaI ACNGT 2 cut(s) 471, 546
TaiI ACGT 3 cut(s) 102, 282, 459
TaqI TCGA 3 cut(s) 195, 294, 349
TasI AATT 7 cut(s) 317, 321, 351, 431, 482, 577, 628
TauI GCSGC 2 cut(s) 82, 85
TfiI GAWTC 1 cut(s) 135
Tru1I TTAA 3 cut(s) 320, 435, 576
Tru9I TTAA 3 cut(s) 320, 435, 576
TscAI CASTG 2 cut(s) 170, 476
TseFI GTSAC 1 cut(s) 142
TseI GCWGC 2 cut(s) 40, 386
Tsp45I GTSAC 1 cut(s) 142
TspDTI ATGAA 1 cut(s) 232
TspGWI ACGGA 1 cut(s) 172
TspRI CASTG 2 cut(s) 170, 476
Van91I CCANNNNNTGG 1 cut(s) 165
VneI GTGCAC 1 cut(s) 306
VpaK11BI GGWCC 2 cut(s) 119, 180
VspI ATTAAT 2 cut(s) 320, 576
XapI RAATTY 2 cut(s) 351, 431
XceI RCATGY 1 cut(s) 314
XmiI GTMKAC 1 cut(s) 548
XspI CTAG 2 cut(s) 390, 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.