Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0009885

Physical Location & Seq

Genomic Coordinates Reverse (-)

18569722 .. 18573878

Gene Structure 4157 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g14980.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1617 bp

ATGGACACCACATCTCTGGATGCAAAACGCCGGCCATTTTCACTCAAAATGTGGCCTCCAAGCCAAAATACGAGGAGAGTGCTTGTGGAGCGGATGACAAACAATCTCAGCACTAAATCCATTTTGACTGAAAAGTATGGCACTCTGAGTAAGGAGGAGGCTGAGGAAAATGCAAAACAGATTGAGGATGTGGCTTTTGTCGCTGCAGACCAGAAGTATGAAAAGGAACCAGATGGCGATGGAAGTTCTGCAGTGCACTGTTATGCTCGGGAATGTAGTAAGATGCTGTTGGAAGTTCTCAAAAAGGGATCTAGTAGCAAGGAGAGCAAAGTCGTATCTGACTCTACACCTAAACAAACTTTGTTTGATATATCTAAAGGCACACGGGCTTTTATTGAAGCTGAGGAAGCTGAGAAACTCTTAAGCCCTTTGAAGGAACCGGCGAATGCCTACACTAAAATATGCTTCAGCAATAGAAGCTTTGGTTTAGGAGCAGCCCGTGTCGCTGAGCCCATTTTGATTTCCCTCAAGAATCAGTTGAAAGAAGTTGACTTATCAGATTTTATAGCAGGTAGACCTGAGGCTGAAGCTCTTGAAGTCATCAATATATTTGCAGCTGCCTTGGAAGGTAGTGAATTGAGATTTCTGGATCTCTCAAACAATGCCTTGGGCGAGAAAGGTGTTAGGGCGTTTGGAGCACTCCTGAAATCACAAAGTTGCTTGGAGGAACTCTATTTGATGAATGATGGCATTTCAGAAGAAGCTGCTCAAGCAGTTCACAAGTTGATTCCTTCTACGGATAAGCTTAAAATCCTTCATTTTCATAATAACATGACAGGAAATGAGGGGGCATTTGCTATTTCTAAGATTGTGAAGCATTCTTCCCTTCTGGAGGATTTCCGTTGTTCCTCCACGAGGGTAGGCTCTGAGGGAGGAGTTGCCTTATGTGAGGCACTGGGGACTTGTACCCACTTAAAGAAGCTGGACTTGCGGGACAACATGTTTGGCGTAGAAGGTGGAATTGCATTGAGTAGAGCTCTTTCCAAGCATGAACATTTGAAAGAGATTTACCTCAGTTACCTGAACCTTGAAGATGAGGGTGTAGTTGCACTAGCCAATGTTCTTAAGGAATCAGCTCCTTCTCTTGAAGTCTTGGACATAGCTGGCAATGACATTACAGCTAAAGCTGCTCCTGCTCTGGCAGCATGTATAGGAGCAAAGACACTTCTCACTCAGCTGAATTTGGGTGAGAATGAACTCAAGGATGAAGGTACTATTCAGATCAGCCAGGTGTTAGAGGGTCTTTCCCAGTTGAAAGAAGTTGACTTGAACACCAACTCAATAAGAAGAGCTGGAGCTCGAGTGTTGGCACAGGCTGTGGTTCAGAAACCTGATTTTAAGTTGTTGAATATCAATGGAAATTTCATATCTGATGATGGCATAGATGAGGTGAAGGCTTTATTCAAGAAATCTCCTCACATGCTTGGCTCTTTGGATGAGAATGACCCTGAAGGAGAGGACGATGCTGAAGATGACGAAGAATCTGGAGATGATGAAGGTAATGAGGACGTGTTAGAATCAAAGCTGAAGAATCTTGAAGTTGACCAAGATGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005819 GO:0005856 GO:0006810 GO:0006913 GO:0006950 GO:0006970 GO:0008150 GO:0009507 GO:0009524 GO:0009536 GO:0009628 GO:0009651 GO:0012505 GO:0015630 GO:0016020 GO:0031090 GO:0031965 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0050896 GO:0051169 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

LRR_6 WPP

Protein Analysis

539

Amino Acids

58.65

Weight (kDa)

4.82

Isoelectric Point (pI)

35.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WPP	PF13943	14 - 109	3.8e-36	WPP domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009885)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06000 AT5G19320 AT5G19320
fragaria_vesca	FvH4_4g14980 FvH4_4g14980
malus_domestica	MD13G1187000.v1.1 MD16G1187900.v1.1
prunus_persica	Prupe.1G154700_v2.0.a1 Prupe.1G154700_v2.0.a1
pyrus_communis	pycom13g16200 pycom16g15790
rosa_chinensis	RchiOBHm_Chr4g0416981
rosa_laevigata	RLG00000007933
rosa_multiflora	Rmu_ssc0000010.1_g000017
rosa_roxburghii	Rroxscaffold_5G00360860
rosa_rugosa	Rorug04G0145800
rosa_samantha	Rh4AG208300 Rh4BG205500 Rh4CG219200 Rh4DG205900
rosa_wichuraiana	Rw4G017660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	560
AccBSI	CCGCTC	1 cut(s)	91
AccI	GTMKAC	1 cut(s)	574
AciI	CCGC	2 cut(s)	91, 991
AclWI	GGATC	2 cut(s)	316, 657
AcoI	YGGCCR	1 cut(s)	32
AcsI	RAATTY	2 cut(s)	1240, 1420
AcuI	CTGAAG	5 cut(s)	451, 606, 1530, 1548, 1607
AfaI	GTAC	2 cut(s)	967, 1273
AfiI	CCNNNNNNNGG	3 cut(s)	433, 892, 915
AflII	CTTAAG	2 cut(s)	421, 1124
AflIII	ACRYGT	2 cut(s)	999, 1569
AjiI	CACGTC	1 cut(s)	1570
AjnI	CCWGG	1 cut(s)	1287
Alw21I	GWGCWC	4 cut(s)	258, 700, 1039, 1360
Alw44I	GTGCAC	1 cut(s)	254
AlwI	GGATC	2 cut(s)	316, 657
Ama87I	CYCGRG	2 cut(s)	267, 1359
AoxI	GGCC	2 cut(s)	32, 53
ApaLI	GTGCAC	1 cut(s)	254
ApeKI	GCWGC	7 cut(s)	203, 494, 614, 617, 764, 1187, 1202
ApoI	RAATTY	2 cut(s)	1240, 1420
ArsI	GACNNNNNNTTYG	2 cut(s)	986, 1018
AsuHPI	GGTGA	2 cut(s)	1259, 1462
AvaI	CYCGRG	2 cut(s)	267, 1359
AxyI	CCTNAGG	1 cut(s)	579
BaeGI	GKGCMC	1 cut(s)	258
BanII	GRGCYC	3 cut(s)	513, 1039, 1360
BauI	CACGAG	1 cut(s)	913
Bbv12I	GWGCWC	4 cut(s)	258, 700, 1039, 1360
BbvCI	CCTCAGC	2 cut(s)	162, 402
BbvI	GCAGC	7 cut(s)	190, 506, 604, 626, 751, 1174, 1214
BccI	CCATC	4 cut(s)	227, 233, 740, 1430
BcgI	CGANNNNNNTGC	2 cut(s)	61, 95
BciT130I	CCWGG	1 cut(s)	1289
BfaI	CTAG	2 cut(s)	312, 1112
BfmI	CTRYAG	2 cut(s)	204, 249
BfrI	CTTAAG	2 cut(s)	421, 1124
BfuAI	ACCTGC	1 cut(s)	560
BisI	GCNGC	7 cut(s)	204, 495, 615, 618, 765, 1188, 1203
BlpI	GCTNAGC	1 cut(s)	507
BlsI	GCNGC	7 cut(s)	205, 496, 616, 619, 766, 1189, 1204
Bme1390I	CCNGG	1 cut(s)	1289
BmeT110I	CYCGRG	2 cut(s)	267, 1359
BmgBI	CACGTC	1 cut(s)	1570
BmiI	GGNNCC	2 cut(s)	228, 438
BmrFI	CCNGG	1 cut(s)	1289
BmrI	ACTGGG	2 cut(s)	965, 1303
BmsI	GCATC	3 cut(s)	10, 273, 1513
BmuI	ACTGGG	2 cut(s)	965, 1303
BplI	GAGNNNNNCTC	2 cut(s)	1021, 1053
BpmI	CTGGAG	3 cut(s)	911, 1374, 1566
Bpu10I	CCTNAGC	2 cut(s)	162, 402
Bpu1102I	GCTNAGC	1 cut(s)	507
BpuEI	CTTGAG	3 cut(s)	512, 753, 1244
BsaJI	CCNNGG	2 cut(s)	621, 666
BsaXI	ACNNNNNCTCC	2 cut(s)	1347, 1377
Bsc4I	CCNNNNNNNGG	3 cut(s)	433, 892, 915
Bse118I	RCCGGY	2 cut(s)	30, 439
Bse1I	ACTGG	2 cut(s)	960, 1309
Bse21I	CCTNAGG	1 cut(s)	579
Bse3DI	GCAATG	1 cut(s)	1174
BseBI	CCWGG	1 cut(s)	1289
BseDI	CCNNGG	2 cut(s)	621, 666
BseGI	GGATG	5 cut(s)	25, 99, 193, 1270, 1501
BseLI	CCNNNNNNNGG	3 cut(s)	433, 892, 915
BseMI	GCAATG	1 cut(s)	1174
BseNI	ACTGG	2 cut(s)	960, 1309
BseRI	GAGGAG	4 cut(s)	88, 170, 948, 1464
BseSI	GKGCMC	1 cut(s)	258
BseXI	GCAGC	7 cut(s)	190, 506, 604, 626, 751, 1174, 1214
BshFI	GGCC	2 cut(s)	34, 55
BsiHKAI	GWGCWC	4 cut(s)	258, 700, 1039, 1360
BsiHKCI	CYCGRG	2 cut(s)	267, 1359
BsiSI	CCGG	2 cut(s)	31, 440
BslFI	GGGAC	2 cut(s)	973, 1007
BslI	CCNNNNNNNGG	3 cut(s)	433, 892, 915
BsmFI	GGGAC	2 cut(s)	973, 1007
BsmI	GAATGC	2 cut(s)	451, 877
BsnI	GGCC	2 cut(s)	34, 55
BsoBI	CYCGRG	2 cut(s)	267, 1359
Bsp1286I	GDGCHC	5 cut(s)	258, 513, 700, 1039, 1360
Bsp143I	GATC	3 cut(s)	308, 649, 1281
Bsp1720I	GCTNAGC	1 cut(s)	507
BspACI	CCGC	2 cut(s)	91, 991
BspANI	GGCC	2 cut(s)	34, 55
BspLI	GGNNCC	2 cut(s)	228, 438
BspMAI	CTGCAG	2 cut(s)	208, 253
BspMI	ACCTGC	1 cut(s)	560
BspPI	GGATC	2 cut(s)	316, 657
BspQI	GCTCTTC	1 cut(s)	1342
BspTI	CTTAAG	2 cut(s)	421, 1124
BsrBI	CCGCTC	1 cut(s)	91
BsrDI	GCAATG	1 cut(s)	1174
BsrFI	RCCGGY	2 cut(s)	30, 439
BsrI	ACTGG	2 cut(s)	960, 1309
BssAI	RCCGGY	2 cut(s)	30, 439
BssECI	CCNNGG	2 cut(s)	621, 666
BssMI	GATC	3 cut(s)	308, 649, 1281
BssSI	CACGAG	1 cut(s)	913
BssT1I	CCWWGG	2 cut(s)	621, 666
Bst2BI	CACGAG	1 cut(s)	913
Bst2UI	CCWGG	1 cut(s)	1289
Bst4CI	ACNGT	1 cut(s)	260
Bst6I	CTCTTC	1 cut(s)	1342
BstAFI	CTTAAG	2 cut(s)	421, 1124
BstC8I	GCNNGC	2 cut(s)	32, 1165
BstENI	CCTNNNNNAGG	2 cut(s)	890, 913
BstF5I	GGATG	5 cut(s)	25, 99, 193, 1270, 1501
BstKTI	GATC	3 cut(s)	311, 652, 1284
BstMBI	GATC	3 cut(s)	308, 649, 1281
BstNI	CCWGG	1 cut(s)	1289
BstNSI	RCATGY	3 cut(s)	1003, 1209, 1483
BstSCI	CCNGG	1 cut(s)	1287
BstSFI	CTRYAG	2 cut(s)	204, 249
BstSLI	GKGCMC	1 cut(s)	258
BstV1I	GCAGC	7 cut(s)	190, 506, 604, 626, 751, 1174, 1214
BstX2I	RGATCY	2 cut(s)	308, 649
BstXI	CCANNNNNNTGG	1 cut(s)	16
BstYI	RGATCY	2 cut(s)	308, 649
Bsu36I	CCTNAGG	1 cut(s)	579
BsuRI	GGCC	2 cut(s)	34, 55
BtgZI	GCGATG	1 cut(s)	252
BtrI	CACGTC	1 cut(s)	1570
BtsCI	GGATG	5 cut(s)	25, 99, 193, 1270, 1501
BtsI	GCAGTG	1 cut(s)	258
BtsIMutI	CAGTG	3 cut(s)	256, 258, 953
BveI	ACCTGC	1 cut(s)	560
Cac8I	GCNNGC	2 cut(s)	32, 1165
Cfr10I	RCCGGY	2 cut(s)	30, 439
Csp6I	GTAC	2 cut(s)	966, 1272
CviAII	CATG	5 cut(s)	832, 1000, 1049, 1206, 1480
CviQI	GTAC	2 cut(s)	966, 1272
DpnI	GATC	3 cut(s)	310, 651, 1283
DpnII	GATC	3 cut(s)	308, 649, 1281
EaeI	YGGCCR	1 cut(s)	32
Eam1104I	CTCTTC	1 cut(s)	1342
EarI	CTCTTC	1 cut(s)	1342
Ecl136II	GAGCTC	2 cut(s)	1037, 1358
Eco130I	CCWWGG	2 cut(s)	621, 666
Eco24I	GRGCYC	3 cut(s)	513, 1039, 1360
Eco53kI	GAGCTC	2 cut(s)	1037, 1358
Eco57I	CTGAAG	5 cut(s)	451, 606, 1530, 1548, 1607
Eco81I	CCTNAGG	1 cut(s)	579
Eco88I	CYCGRG	2 cut(s)	267, 1359
EcoICRI	GAGCTC	2 cut(s)	1037, 1358
EcoNI	CCTNNNNNAGG	2 cut(s)	890, 913
EcoRII	CCWGG	1 cut(s)	1287
EcoT14I	CCWWGG	2 cut(s)	621, 666
EcoT38I	GRGCYC	3 cut(s)	513, 1039, 1360
ErhI	CCWWGG	2 cut(s)	621, 666
FaeI	CATG	5 cut(s)	835, 1003, 1052, 1209, 1483
FalI	AAGNNNNNCTT	2 cut(s)	971, 1003
FaqI	GGGAC	2 cut(s)	973, 1007
FatI	CATG	5 cut(s)	831, 999, 1048, 1205, 1479
FauI	CCCGC	1 cut(s)	984
FblI	GTMKAC	1 cut(s)	574
Fnu4HI	GCNGC	7 cut(s)	204, 495, 615, 618, 765, 1188, 1203
FokI	GGATG	5 cut(s)	32, 106, 200, 1277, 1508
FriOI	GRGCYC	3 cut(s)	513, 1039, 1360
Fsp4HI	GCNGC	7 cut(s)	204, 495, 615, 618, 765, 1188, 1203
FspBI	CTAG	2 cut(s)	312, 1112
GluI	GCNGC	7 cut(s)	204, 495, 615, 618, 765, 1188, 1203
GsuI	CTGGAG	3 cut(s)	911, 1374, 1566
HaeIII	GGCC	2 cut(s)	34, 55
HapII	CCGG	2 cut(s)	31, 440
Hin1II	CATG	5 cut(s)	835, 1003, 1052, 1209, 1483
HincII	GTYRAC	3 cut(s)	550, 1324, 1603
HindII	GTYRAC	3 cut(s)	550, 1324, 1603
HindIII	AAGCTT	2 cut(s)	478, 803
HinfI	GANTC	7 cut(s)	341, 532, 787, 1130, 1541, 1577, 1591
HpaII	CCGG	2 cut(s)	31, 440
HphI	GGTGA	2 cut(s)	1259, 1462
Hpy166II	GTNNAC	6 cut(s)	256, 550, 575, 778, 1324, 1603
Hpy188I	TCNGA	8 cut(s)	147, 340, 559, 757, 928, 1281, 1386, 1432
Hpy8I	GTNNAC	6 cut(s)	256, 550, 575, 778, 1324, 1603
HpyCH4III	ACNGT	1 cut(s)	260
HpyCH4IV	ACGT	1 cut(s)	1569
HpyCH4V	TGCA	8 cut(s)	23, 173, 206, 251, 256, 614, 1025, 1109
HpySE526I	ACGT	1 cut(s)	1569
Hsp92II	CATG	5 cut(s)	835, 1003, 1052, 1209, 1483
KroI	GCCGGC	1 cut(s)	30
KroNI	GCCGGC	1 cut(s)	32
Kzo9I	GATC	3 cut(s)	308, 649, 1281
LguI	GCTCTTC	1 cut(s)	1342
LmnI	GCTCC	7 cut(s)	88, 491, 695, 1141, 1195, 1214, 1355
Lsp1109I	GCAGC	7 cut(s)	190, 506, 604, 626, 751, 1174, 1214
LweI	GCATC	3 cut(s)	10, 273, 1513
MaeI	CTAG	2 cut(s)	312, 1112
MaeII	ACGT	1 cut(s)	1569
MaeIII	GTNAC	1 cut(s)	1076
MalI	GATC	3 cut(s)	310, 651, 1283
MbiI	CCGCTC	1 cut(s)	91
MboI	GATC	3 cut(s)	308, 649, 1281
MboII	GAAGA	7 cut(s)	770, 873, 1103, 1359, 1541, 1550, 1600
MflI	RGATCY	2 cut(s)	308, 649
MhlI	GDGCHC	5 cut(s)	258, 513, 700, 1039, 1360
MluCI	AATT	4 cut(s)	635, 1020, 1240, 1420
MlyI	GAGTC	1 cut(s)	335
MmeI	TCCRAC	1 cut(s)	270
MroNI	GCCGGC	1 cut(s)	30
MseI	TTAA	5 cut(s)	422, 807, 974, 1125, 1398
MslI	CAYNNNNRTG	1 cut(s)	261
MspA1I	CMGCKG	2 cut(s)	617, 1237
MspCI	CTTAAG	2 cut(s)	421, 1124
MspI	CCGG	2 cut(s)	31, 440
MspR9I	CCNGG	1 cut(s)	1289
Mva1269I	GAATGC	2 cut(s)	451, 877
MvaI	CCWGG	1 cut(s)	1289
NaeI	GCCGGC	1 cut(s)	32
NdeII	GATC	3 cut(s)	308, 649, 1281
NgoMIV	GCCGGC	1 cut(s)	30
NlaIII	CATG	5 cut(s)	835, 1003, 1052, 1209, 1483
NlaIV	GGNNCC	2 cut(s)	228, 438
NspI	RCATGY	3 cut(s)	1003, 1209, 1483
PaeR7I	CTCGAG	1 cut(s)	1359
PciI	ACATGT	1 cut(s)	999
PciSI	GCTCTTC	1 cut(s)	1342
PctI	GAATGC	2 cut(s)	451, 877
PdiI	GCCGGC	1 cut(s)	32
PfeI	GAWTC	6 cut(s)	532, 787, 1130, 1541, 1577, 1591
PkrI	GCNGC	7 cut(s)	205, 496, 616, 619, 766, 1189, 1204
PleI	GAGTC	1 cut(s)	335
PpsI	GAGTC	1 cut(s)	335
PscI	ACATGT	1 cut(s)	999
Psp124BI	GAGCTC	2 cut(s)	1039, 1360
Psp6I	CCWGG	1 cut(s)	1287
PspGI	CCWGG	1 cut(s)	1287
PspN4I	GGNNCC	2 cut(s)	228, 438
PspXI	VCTCGAGB	1 cut(s)	1359
PstI	CTGCAG	2 cut(s)	208, 253
PsuI	RGATCY	2 cut(s)	308, 649
PvuII	CAGCTG	2 cut(s)	617, 1237
RsaI	GTAC	2 cut(s)	967, 1273
RsaNI	GTAC	2 cut(s)	966, 1272
RseI	CAYNNNNRTG	1 cut(s)	261
SacI	GAGCTC	2 cut(s)	1039, 1360
SapI	GCTCTTC	1 cut(s)	1342
SaqAI	TTAA	5 cut(s)	422, 807, 974, 1125, 1398
SatI	GCNGC	7 cut(s)	204, 495, 615, 618, 765, 1188, 1203
Sau3AI	GATC	3 cut(s)	308, 649, 1281
SchI	GAGTC	1 cut(s)	335
ScrFI	CCNGG	1 cut(s)	1289
SduI	GDGCHC	5 cut(s)	258, 513, 700, 1039, 1360
SfaNI	GCATC	3 cut(s)	10, 273, 1513
SfcI	CTRYAG	2 cut(s)	204, 249
Sfr274I	CTCGAG	1 cut(s)	1359
SlaI	CTCGAG	1 cut(s)	1359
SmiMI	CAYNNNNRTG	1 cut(s)	261
SmlI	CTYRAG	6 cut(s)	421, 527, 768, 1124, 1259, 1359
SmoI	CTYRAG	6 cut(s)	421, 527, 768, 1124, 1259, 1359
Sse9I	AATT	4 cut(s)	635, 1020, 1240, 1420
SsiI	CCGC	2 cut(s)	91, 991
SspMI	CTAG	2 cut(s)	312, 1112
SstI	GAGCTC	2 cut(s)	1039, 1360
StyD4I	CCNGG	1 cut(s)	1287
StyI	CCWWGG	2 cut(s)	621, 666
TaaI	ACNGT	1 cut(s)	260
TaiI	ACGT	1 cut(s)	1572
TaqI	TCGA	1 cut(s)	1360
TasI	AATT	4 cut(s)	635, 1020, 1240, 1420
TfiI	GAWTC	6 cut(s)	532, 787, 1130, 1541, 1577, 1591
Tru1I	TTAA	5 cut(s)	422, 807, 974, 1125, 1398
Tru9I	TTAA	5 cut(s)	422, 807, 974, 1125, 1398
TscAI	CASTG	3 cut(s)	258, 263, 960
TseI	GCWGC	7 cut(s)	203, 494, 614, 617, 764, 1187, 1202
TspDTI	ATGAA	9 cut(s)	234, 755, 806, 812, 1065, 1269, 1281, 1414, 1569
TspGWI	ACGGA	2 cut(s)	812, 890
TspRI	CASTG	3 cut(s)	258, 263, 960
Vha464I	CTTAAG	2 cut(s)	421, 1124
VneI	GTGCAC	1 cut(s)	254
XagI	CCTNNNNNAGG	2 cut(s)	890, 913
XapI	RAATTY	2 cut(s)	1240, 1420
XceI	RCATGY	3 cut(s)	1003, 1209, 1483
XhoI	CTCGAG	1 cut(s)	1359
XmiI	GTMKAC	1 cut(s)	574
XspI	CTAG	2 cut(s)	312, 1112

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.