FvH4_4g20780

30S ribosomal protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0013214
Physical Location & Seq
Reverse (-)
23912180 .. 23914801
2622 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g20780.t2

Sequence Viewer

Length: 1230 bp
ATGGCGTCTTTGGCTCAGCAATTCACAGGCTTGAGATGCTCCCCTCTCTCCACTTCTCGGCTCTCCAAGCCCTTCTCTCCCACCCAAACCAAGACCACTCTCCTCCCCATTGTCTCTGCTTCCGTCATCTCCAACGCTCAGACCAAAGAGCGCCTCAAGCTCAAGGAAATCTTTGAAGAGGCCCAGGAACGGTGCCGTACTGCCCCCATGGAAGGCGTCTCCTTCAACCTCGACTCTTTCTACTCCGCTCTTGAAAAGTACGACTTCAACTCCGAGATTGGCACCAAGGTGAAGGGTGTGATTTTCCATACAGATAACAAGGGGGCATTAGTCGACATTACTGCAAAGTCTTCGGCACATTTGAAAGTGGAAGATGCATGTATGCACAAGATAAAGCATGTTGAAGAAGTGGGGTTAGTTCCCGGTGTGAAAGAGGAGTTTGTCATTATCGGTGAAAATGAGGACGATGACAGCTTGATCTTGAGCTTGAAGACTATACAGTTTGAGCTTGCATGGGAGAGGTGTAGGCAGCTCCAAGCTGAGGATGTTGTTGTCAAGGGTAGGGTTGTTGGTGCAAACAAAGGTGGAGTGGTGGCTGTGGTGGAAGGCCTTCGAGGTTTTGTTCCTTTCTCGCAGTTATCAACAAAATCAACTGCGGAAGAGCTTATTGAGAAGGATATCCCTCTGAAGTTTGTGGAGGTTGATGAGGAACAGTCTAGGCTTGTTCTCAGTAACCGCAAGGCCATGGCAGACAATCAAGCACAACTTGGAATTGGATCAGTTGTCCTTGGAACTGTCCAGAGTCTGAAGCCATATGGTGCATTTATTGACATTGGTGGAATCAACGGTCTTCTTCACGTTAGTCAGATCAGTCATGACCGAGTCTCTGATATTGCAACAGTTCTCCAACCTGGTGACACTCTCAAGGTCATGATATTGAGCCATGACCGTGAGAGAGGCCGTGTAAGTCTATCTACTAAGAAGTTAGAACCAACTCCTGGTGACATGATTCGCAATCCTACACTCGTTTTTGAGAAGGCTGAGGAGATGGCTCAGACATTCAGGCAGAGAATTGCTCAAGCAGAGGCTATGGCTCGTGCAGATATGCTTCGTTTCCAACCAGAGAGCGGATTAACTCTCAGCTCTGATGGGATCCTAGGCCCAGTTACTTCAGACCTACCAATAGAGGGTTTAGACTTGAGTGATGTGCCTTCAGCTGTGACCGAGTGA

Protein Analysis

410

Amino Acids

44.68

Weight (kDa)

5.33

Isoelectric Point (pI)

38.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 286 - 355 8.1e-18 S1 RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013214)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G30510
fragaria_vesca FvH4_4g20780 FvH4_4g20780
malus_domestica MD13G1147900.v1.1 MD16G1147700.v1.1
prunus_persica Prupe.1G111400_v2.0.a1
pyrus_communis pycom16g12610 pycom16g12620
rosa_chinensis RchiOBHm_Chr4g0426221
rosa_laevigata RLG00000007309
rosa_multiflora Rmu_sc0000730.1_g000026
rosa_roxburghii Rroxscaffold_5G00368240
rosa_rugosa Rorug04G0209700
rosa_samantha Rh4AG266600 Rh4BG272600 Rh4CG285800 Rh4DG268900
rosa_wichuraiana Rw4G023050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 192, 281
AccB7I CCANNNNNTGG 1 cut(s) 998
AccBSI CCGCTC 2 cut(s) 248, 1128
AccI GTMKAC 1 cut(s) 333
AciI CCGC 4 cut(s) 246, 656, 736, 1128
AclWI GGATC 3 cut(s) 784, 1147, 1160
AcuI CTGAAG 4 cut(s) 707, 827, 1155, 1197
AcyI GRCGYC 2 cut(s) 5, 216
AfaI GTAC 2 cut(s) 199, 260
AfiI CCNNNNNNNGG 7 cut(s) 57, 189, 212, 541, 998, 1127, 1187
AgsI TTSAA 7 cut(s) 176, 226, 254, 268, 364, 404, 490
AjnI CCWGG 3 cut(s) 183, 910, 997
AleI CACNNNNGTG 1 cut(s) 287
AluBI AGCT 9 cut(s) 160, 474, 486, 508, 532, 539, 664, 1143, 1217
AluI AGCT 9 cut(s) 160, 474, 486, 508, 532, 539, 664, 1143, 1217
Alw26I GTCTC 3 cut(s) 118, 223, 889
AlwI GGATC 3 cut(s) 784, 1147, 1160
AlwNI CAGNNNCTG 1 cut(s) 805
AoxI GGCC 5 cut(s) 180, 607, 741, 958, 1159
ApeKI GCWGC 1 cut(s) 529
Asp700I GAANNNNTTC 1 cut(s) 609
AspA2I CCTAGG 1 cut(s) 1156
AspLEI GCGC 1 cut(s) 153
AspS9I GGNCC 2 cut(s) 181, 1160
AsuC2I CCSGG 1 cut(s) 423
AsuHPI GGTGA 4 cut(s) 301, 464, 926, 1013
AvrII CCTAGG 1 cut(s) 1156
BamHI GGATCC 1 cut(s) 1152
BanI GGYRCC 2 cut(s) 192, 281
BauI CACGAG 1 cut(s) 1095
BbsI GAAGAC 3 cut(s) 342, 497, 842
BbvCI CCTCAGC 2 cut(s) 540, 1041
BbvI GCAGC 1 cut(s) 541
BccI CCATC 2 cut(s) 1042, 1142
BceAI ACGGC 2 cut(s) 180, 945
BcgI CGANNNNNNTGC 4 cut(s) 323, 333, 357, 367
BciT130I CCWGG 3 cut(s) 185, 912, 999
BcnI CCSGG 1 cut(s) 423
BcoDI GTCTC 3 cut(s) 118, 223, 889
BfaI CTAG 2 cut(s) 717, 1157
BfoI RGCGCY 1 cut(s) 154
BisI GCNGC 1 cut(s) 530
BlnI CCTAGG 1 cut(s) 1156
BlpI GCTNAGC 1 cut(s) 15
BlsI GCNGC 1 cut(s) 531
Bme1390I CCNGG 4 cut(s) 185, 423, 912, 999
BmgT120I GGNCC 2 cut(s) 181, 1160
BmiI GGNNCC 3 cut(s) 194, 283, 1154
BmrFI CCNGG 4 cut(s) 185, 423, 912, 999
BmrI ACTGGG 1 cut(s) 1157
BmsI GCATC 2 cut(s) 26, 364
BmuI ACTGGG 1 cut(s) 1157
BpiI GAAGAC 3 cut(s) 342, 497, 842
BplI GAGNNNNNCTC 2 cut(s) 1060, 1092
Bpu10I CCTNAGC 2 cut(s) 540, 1041
Bpu1102I GCTNAGC 1 cut(s) 15
BpuEI CTTGAG 7 cut(s) 52, 140, 146, 502, 908, 1062, 1219
BpuMI CCSGG 1 cut(s) 423
BsaHI GRCGYC 2 cut(s) 5, 216
BsaJI CCNNGG 6 cut(s) 183, 207, 285, 744, 787, 1156
BsaXI ACNNNNNCTCC 2 cut(s) 254, 284
Bsc4I CCNNNNNNNGG 7 cut(s) 57, 189, 212, 541, 998, 1127, 1187
Bse1I ACTGG 1 cut(s) 1163
BseBI CCWGG 3 cut(s) 185, 912, 999
BseDI CCNNGG 6 cut(s) 183, 207, 285, 744, 787, 1156
BseGI GGATG 1 cut(s) 550
BseLI CCNNNNNNNGG 7 cut(s) 57, 189, 212, 541, 998, 1127, 1187
BseMII CTCAG 7 cut(s) 29, 152, 531, 742, 1032, 1067, 1153
BseNI ACTGG 1 cut(s) 1163
BseRI GAGGAG 3 cut(s) 92, 449, 1058
BseXI GCAGC 1 cut(s) 541
BsgI GTGCAG 1 cut(s) 1119
BshFI GGCC 5 cut(s) 182, 609, 743, 960, 1161
BshNI GGYRCC 2 cut(s) 192, 281
BsiSI CCGG 1 cut(s) 423
BslI CCNNNNNNNGG 7 cut(s) 57, 189, 212, 541, 998, 1127, 1187
BsmAI GTCTC 3 cut(s) 118, 223, 889
BsmBI CGTCTC 1 cut(s) 223
BsnI GGCC 5 cut(s) 182, 609, 743, 960, 1161
Bsp143I GATC 4 cut(s) 477, 776, 867, 1152
Bsp1720I GCTNAGC 1 cut(s) 15
Bsp19I CCATGG 2 cut(s) 207, 744
BspACI CCGC 4 cut(s) 246, 656, 736, 1128
BspANI GGCC 5 cut(s) 182, 609, 743, 960, 1161
BspCNI CTCAG 7 cut(s) 28, 151, 532, 741, 1033, 1066, 1152
BspHI TCATGA 2 cut(s) 874, 930
BspLI GGNNCC 3 cut(s) 194, 283, 1154
BspPI GGATC 3 cut(s) 784, 1147, 1160
BspQI GCTCTTC 1 cut(s) 654
BspT107I GGYRCC 2 cut(s) 192, 281
BsrBI CCGCTC 2 cut(s) 248, 1128
BsrI ACTGG 1 cut(s) 1163
BssECI CCNNGG 6 cut(s) 183, 207, 285, 744, 787, 1156
BssMI GATC 4 cut(s) 477, 776, 867, 1152
BssNI GRCGYC 2 cut(s) 5, 216
BssSI CACGAG 1 cut(s) 1095
BssT1I CCWWGG 5 cut(s) 207, 285, 744, 787, 1156
Bst2BI CACGAG 1 cut(s) 1095
Bst2UI CCWGG 3 cut(s) 185, 912, 999
Bst4CI ACNGT 7 cut(s) 192, 501, 714, 796, 848, 901, 950
Bst6I CTCTTC 2 cut(s) 171, 654
BstACI GRCGYC 2 cut(s) 5, 216
BstC8I GCNNGC 1 cut(s) 510
BstDEI CTNAG 8 cut(s) 15, 138, 540, 728, 978, 1041, 1053, 1139
BstDSI CCRYGG 2 cut(s) 207, 744
BstF5I GGATG 1 cut(s) 550
BstH2I RGCGCY 1 cut(s) 154
BstHHI GCGC 1 cut(s) 153
BstKTI GATC 4 cut(s) 480, 779, 870, 1155
BstMAI GTCTC 3 cut(s) 118, 223, 889
BstMBI GATC 4 cut(s) 477, 776, 867, 1152
BstMWI GCNNNNNNNGC 4 cut(s) 11, 36, 67, 157
BstNI CCWGG 3 cut(s) 185, 912, 999
BstNSI RCATGY 2 cut(s) 381, 401
BstSCI CCNGG 4 cut(s) 183, 421, 910, 997
BstV1I GCAGC 1 cut(s) 541
BstV2I GAAGAC 3 cut(s) 342, 497, 842
BstX2I RGATCY 1 cut(s) 1152
BstYI RGATCY 1 cut(s) 1152
BsuRI GGCC 5 cut(s) 182, 609, 743, 960, 1161
BtgI CCRYGG 2 cut(s) 207, 744
BtsCI GGATG 1 cut(s) 550
Cac8I GCNNGC 1 cut(s) 510
CaiI CAGNNNCTG 1 cut(s) 805
CciI TCATGA 2 cut(s) 874, 930
CfoI GCGC 1 cut(s) 153
Cfr13I GGNCC 2 cut(s) 181, 1160
CseI GACGC 1 cut(s) 205
CsiI ACCWGGT 1 cut(s) 910
Csp6I GTAC 2 cut(s) 198, 259
CviAII CATG 9 cut(s) 208, 378, 398, 513, 745, 875, 931, 944, 1006
CviQI GTAC 2 cut(s) 198, 259
DdeI CTNAG 8 cut(s) 15, 138, 540, 728, 978, 1041, 1053, 1139
DpnI GATC 4 cut(s) 479, 778, 869, 1154
DpnII GATC 4 cut(s) 477, 776, 867, 1152
Eam1104I CTCTTC 2 cut(s) 171, 654
EarI CTCTTC 2 cut(s) 171, 654
Eco130I CCWWGG 5 cut(s) 207, 285, 744, 787, 1156
Eco147I AGGCCT 1 cut(s) 609
Eco32I GATATC 1 cut(s) 679
Eco57I CTGAAG 4 cut(s) 707, 827, 1155, 1197
EcoRII CCWGG 3 cut(s) 183, 910, 997
EcoRV GATATC 1 cut(s) 679
EcoT14I CCWWGG 5 cut(s) 207, 285, 744, 787, 1156
EcoT22I ATGCAT 1 cut(s) 379
ErhI CCWWGG 5 cut(s) 207, 285, 744, 787, 1156
Esp3I CGTCTC 1 cut(s) 223
FaeI CATG 9 cut(s) 211, 381, 401, 516, 748, 878, 934, 947, 1009
FalI AAGNNNNNCTT 6 cut(s) 155, 187, 248, 280, 750, 782
FatI CATG 9 cut(s) 207, 377, 397, 512, 744, 874, 930, 943, 1005
FauNDI CATATG 1 cut(s) 814
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 1 cut(s) 530
FokI GGATG 1 cut(s) 557
Fsp4HI GCNGC 1 cut(s) 530
FspBI CTAG 2 cut(s) 717, 1157
GlaI GCGC 1 cut(s) 152
GluI GCNGC 1 cut(s) 530
HaeII RGCGCY 1 cut(s) 154
HaeIII GGCC 5 cut(s) 182, 609, 743, 960, 1161
HapII CCGG 1 cut(s) 423
HgaI GACGC 1 cut(s) 205
HhaI GCGC 1 cut(s) 153
Hin1I GRCGYC 2 cut(s) 5, 216
Hin1II CATG 9 cut(s) 211, 381, 401, 516, 748, 878, 934, 947, 1009
Hin6I GCGC 1 cut(s) 151
HinP1I GCGC 1 cut(s) 151
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HinfI GANTC 5 cut(s) 233, 802, 840, 882, 1009
HpaII CCGG 1 cut(s) 423
HphI GGTGA 4 cut(s) 301, 464, 926, 1013
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 9 cut(s) 141, 274, 687, 807, 867, 889, 1056, 1147, 1174
Hpy188III TCNNGA 5 cut(s) 251, 481, 799, 875, 931
Hpy8I GTNNAC 1 cut(s) 334
HpyAV CCTTC 9 cut(s) 82, 206, 232, 286, 599, 620, 667, 1030, 1221
HpyCH4III ACNGT 7 cut(s) 192, 501, 714, 796, 848, 901, 950
HpyCH4IV ACGT 1 cut(s) 858
HpyCH4V TGCA 8 cut(s) 344, 377, 385, 512, 575, 821, 896, 1100
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 36, 67, 157
HpyF3I CTNAG 8 cut(s) 15, 138, 540, 728, 978, 1041, 1053, 1139
HpySE526I ACGT 1 cut(s) 858
Hsp92I GRCGYC 2 cut(s) 5, 216
Hsp92II CATG 9 cut(s) 211, 381, 401, 516, 748, 878, 934, 947, 1009
HspAI GCGC 1 cut(s) 151
Kzo9I GATC 4 cut(s) 477, 776, 867, 1152
LguI GCTCTTC 1 cut(s) 654
LmnI GCTCC 2 cut(s) 44, 537
Lsp1109I GCAGC 1 cut(s) 541
LweI GCATC 2 cut(s) 26, 364
MabI ACCWGGT 1 cut(s) 910
MaeI CTAG 2 cut(s) 717, 1157
MaeII ACGT 1 cut(s) 858
MaeIII GTNAC 5 cut(s) 731, 914, 1001, 1165, 1219
MalI GATC 4 cut(s) 479, 778, 869, 1154
MbiI CCGCTC 2 cut(s) 248, 1128
MboI GATC 4 cut(s) 477, 776, 867, 1152
MboII GAAGA 8 cut(s) 188, 342, 383, 416, 502, 671, 842, 845
MflI RGATCY 1 cut(s) 1152
MluCI AATT 3 cut(s) 20, 771, 1071
MlyI GAGTC 3 cut(s) 227, 811, 891
MmeI TCCRAC 3 cut(s) 156, 931, 1141
Mph1103I ATGCAT 1 cut(s) 379
MroXI GAANNNNTTC 1 cut(s) 609
MseI TTAA 1 cut(s) 1133
MslI CAYNNNNRTG 2 cut(s) 287, 948
MspA1I CMGCKG 1 cut(s) 1217
MspI CCGG 1 cut(s) 423
MspR9I CCNGG 4 cut(s) 185, 423, 912, 999
MvaI CCWGG 3 cut(s) 185, 912, 999
MwoI GCNNNNNNNGC 4 cut(s) 11, 36, 67, 157
NciI CCSGG 1 cut(s) 423
NcoI CCATGG 2 cut(s) 207, 744
NdeI CATATG 1 cut(s) 814
NdeII GATC 4 cut(s) 477, 776, 867, 1152
NlaIII CATG 9 cut(s) 211, 381, 401, 516, 748, 878, 934, 947, 1009
NlaIV GGNNCC 3 cut(s) 194, 283, 1154
NmeAIII GCCGAG 1 cut(s) 37
NmuCI GTSAC 3 cut(s) 914, 1001, 1219
NsiI ATGCAT 1 cut(s) 379
NspI RCATGY 2 cut(s) 381, 401
OliI CACNNNNGTG 1 cut(s) 287
PagI TCATGA 2 cut(s) 874, 930
PceI AGGCCT 1 cut(s) 609
PciSI GCTCTTC 1 cut(s) 654
PdmI GAANNNNTTC 1 cut(s) 609
PfeI GAWTC 2 cut(s) 840, 1009
PflFI GACNNNGTC 1 cut(s) 881
PflMI CCANNNNNTGG 1 cut(s) 998
PkrI GCNGC 1 cut(s) 531
PleI GAGTC 3 cut(s) 227, 810, 890
PpsI GAGTC 3 cut(s) 227, 810, 890
Psp6I CCWGG 3 cut(s) 183, 910, 997
PspGI CCWGG 3 cut(s) 183, 910, 997
PspN4I GGNNCC 3 cut(s) 194, 283, 1154
PspPI GGNCC 2 cut(s) 181, 1160
PstNI CAGNNNCTG 1 cut(s) 805
PsuI RGATCY 1 cut(s) 1152
PsyI GACNNNGTC 1 cut(s) 881
PvuII CAGCTG 1 cut(s) 1217
RsaI GTAC 2 cut(s) 199, 260
RsaNI GTAC 2 cut(s) 198, 259
RseI CAYNNNNRTG 2 cut(s) 287, 948
SalI GTCGAC 1 cut(s) 332
SapI GCTCTTC 1 cut(s) 654
SaqAI TTAA 1 cut(s) 1133
SatI GCNGC 1 cut(s) 530
Sau3AI GATC 4 cut(s) 477, 776, 867, 1152
Sau96I GGNCC 2 cut(s) 181, 1160
SchI GAGTC 3 cut(s) 227, 811, 891
ScrFI CCNGG 4 cut(s) 185, 423, 912, 999
SexAI ACCWGGT 1 cut(s) 910
SfaNI GCATC 2 cut(s) 26, 364
SmiMI CAYNNNNRTG 2 cut(s) 287, 948
SmlI CTYRAG 7 cut(s) 31, 155, 161, 481, 923, 1077, 1198
SmoI CTYRAG 7 cut(s) 31, 155, 161, 481, 923, 1077, 1198
Sse9I AATT 3 cut(s) 20, 771, 1071
SseBI AGGCCT 1 cut(s) 609
SsiI CCGC 4 cut(s) 246, 656, 736, 1128
SspMI CTAG 2 cut(s) 717, 1157
StuI AGGCCT 1 cut(s) 609
StyD4I CCNGG 4 cut(s) 183, 421, 910, 997
StyI CCWWGG 5 cut(s) 207, 285, 744, 787, 1156
TaaI ACNGT 7 cut(s) 192, 501, 714, 796, 848, 901, 950
TaiI ACGT 1 cut(s) 861
TaqI TCGA 3 cut(s) 231, 333, 613
TaqII GACCGA 1 cut(s) 894
TasI AATT 3 cut(s) 20, 771, 1071
TfiI GAWTC 2 cut(s) 840, 1009
Tru1I TTAA 1 cut(s) 1133
Tru9I TTAA 1 cut(s) 1133
TseFI GTSAC 3 cut(s) 914, 1001, 1219
TseI GCWGC 1 cut(s) 529
Tsp45I GTSAC 3 cut(s) 914, 1001, 1219
TspGWI ACGGA 1 cut(s) 112
Tth111I GACNNNGTC 1 cut(s) 881
Van91I CCANNNNNTGG 1 cut(s) 998
XceI RCATGY 2 cut(s) 381, 401
XmaJI CCTAGG 1 cut(s) 1156
XmiI GTMKAC 1 cut(s) 333
XmnI GAANNNNTTC 1 cut(s) 609
XspI CTAG 2 cut(s) 717, 1157
Zsp2I ATGCAT 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.