MD13G1147900.v1.1

30S ribosomal protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013214
Physical Location & Seq
Forward (+)
11557431 .. 11560274
2844 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1147900.v1.1.491

Sequence Viewer

Length: 1239 bp
ATGGCGTCTCTGGCTCAGCAATTCACAGGCTTAAGATGCTCACCGCTCTCCACCTCGAGGCTCTCGAAGCCCTCAATCCTGCCGAAGCAAAACAAAATGCCTGGTTTGCTCCCCATCGTCTCCGCCGCGGTCATCTCGAATGCACAGACCAAAGAGCGCCTCAAGCTCAAGGAAATCTTCGAGGATGCTCACGAACGGTGTCGTACTGCCCCCATGGAAGGTGTCTCTTTCACTCTTGACTCCTTTTACAACGCTCTCGAGAAGTACGACTTCAATTCTGAGATTGGAACTAAGGTGAGGGGAACAGTTTTCAATATAGATAACAATGGAGCATTAGTTGACATTACTGCAAAATCTTCAGCATACTTGCCTCTTGAAGAGGCATGCATTCACAAAATAAAGAGTGTAGAAGAAGTGGGCATAGTTCCCGGAGTGAGAGAGGAGTTTGTGATTATTGGTGAAAACGAAGCTGACGATAGCTTGATCTTGAGCTTAAAGTCCATCCAGTATGACCTAGCATGGGAAAGATGTAGACAGCTCCAAGCTGAGGATGTTGTTGTCAAGGGTAAGGTTGTTGGTGCGAATAAAGGTGGAGTGGTGGCCGTGGTGGAAGGCCTTAGAGGTTTTGTTCCTTTCTCCCAGATATCAACAAAATCAACTGCCGAAGATCTTCTTGAAAAGGAGATTCCTCTGAAGTTTGTGGAGGTTGATGAAGAACAGTCTAGGCTTGTCCTCAGTAACCGCAAGGCCGTGGCAGACAACCAAGCACAGCTTGGAATTGGGTCAGTTGTCCTTGGTACTGTTCAGAGCTTGAAGCCATATGGTGCCTTTATCGACATTGGTGGAATCAACGGCCTTCTTCATGTTAGTCAGATCAGTCATGACCGAGTCTCTGATATTGCGACAGTTCTCCAACCTGGTGACACTCTCAAGGTCATGATATTGAGCCATGACCGTGAGAGAGGCCGTGTAAGTCTTTCTACCAAGAAGTTAGAACCTACTCCCGGTGACATGATTCGCAATCCAAAGCTTGTATTTGAGAAGGCGGAGGAGATGGCACAGACATTCCGGCAGAGAATTGCTCAAGCGGAAGCTATGGCTCGTGCGGACATGCTCCGTTTCCAGCCGGAGAGTGGGTTAACCCTCAGCTCCGACGGAATCTTGGGTCCTCTCACTTCGGACTTGCCAGTCGAGGGTTTAGATTTGAGCGACGTTCCCCCGGCTGAAGTGACTGACTGA

Protein Analysis

413

Amino Acids

44.88

Weight (kDa)

5.2

Isoelectric Point (pI)

37.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 183 - 246 5.2e-09 S1 RNA binding domain
S1 PF00575 260 - 329 7.4e-18 S1 RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013214)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G30510
fragaria_vesca FvH4_4g20780 FvH4_4g20780
malus_domestica MD13G1147900.v1.1 MD16G1147700.v1.1
prunus_persica Prupe.1G111400_v2.0.a1
pyrus_communis pycom16g12610 pycom16g12620
rosa_chinensis RchiOBHm_Chr4g0426221
rosa_laevigata RLG00000007309
rosa_multiflora Rmu_sc0000730.1_g000026
rosa_roxburghii Rroxscaffold_5G00368240
rosa_rugosa Rorug04G0209700
rosa_samantha Rh4AG266600 Rh4BG272600 Rh4CG285800 Rh4DG268900
rosa_wichuraiana Rw4G023050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1187
AbsI CCTCGAGG 1 cut(s) 55
AccB1I GGYRCC 1 cut(s) 824
AccBSI CCGCTC 1 cut(s) 46
AccI GTMKAC 1 cut(s) 532
AccII CGCG 1 cut(s) 128
AciI CCGC 8 cut(s) 44, 123, 126, 128, 742, 1046, 1088, 1106
AcoI YGGCCR 1 cut(s) 600
AcuI CTGAAG 2 cut(s) 342, 713
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 205, 266, 799
AfiI CCNNNNNNNGG 7 cut(s) 57, 218, 520, 547, 1004, 1133, 1193
AflII CTTAAG 1 cut(s) 31
AgsI TTSAA 5 cut(s) 274, 313, 377, 677, 814
AjnI CCWGG 2 cut(s) 100, 916
Alw26I GTCTC 4 cut(s) 12, 124, 229, 895
Ama87I CYCGRG 2 cut(s) 55, 257
AoxI GGCC 5 cut(s) 600, 613, 747, 853, 964
Asp700I GAANNNNTTC 1 cut(s) 669
AspLEI GCGC 1 cut(s) 159
AspS9I GGNCC 1 cut(s) 1166
AsuC2I CCSGG 3 cut(s) 429, 1005, 1220
AsuHPI GGTGA 5 cut(s) 33, 307, 470, 932, 1019
AvaI CYCGRG 2 cut(s) 55, 257
AvaII GGWCC 1 cut(s) 1166
BanI GGYRCC 1 cut(s) 824
BauI CACGAG 1 cut(s) 1101
BbvCI CCTCAGC 2 cut(s) 546, 1145
BccI CCATC 3 cut(s) 122, 509, 1048
BceAI ACGGC 4 cut(s) 587, 734, 868, 951
BciT130I CCWGG 2 cut(s) 102, 918
BcnI CCSGG 3 cut(s) 429, 1005, 1220
BcoDI GTCTC 4 cut(s) 12, 124, 229, 895
BfaI CTAG 2 cut(s) 515, 723
BfoI RGCGCY 1 cut(s) 160
BfrI CTTAAG 1 cut(s) 31
BglII AGATCT 1 cut(s) 667
BisI GCNGC 1 cut(s) 126
BlpI GCTNAGC 1 cut(s) 15
BlsI GCNGC 1 cut(s) 127
Bme1390I CCNGG 5 cut(s) 102, 429, 918, 1005, 1220
Bme18I GGWCC 1 cut(s) 1166
BmeT110I CYCGRG 2 cut(s) 55, 257
BmgT120I GGNCC 1 cut(s) 1166
BmiI GGNNCC 2 cut(s) 826, 1167
BmrFI CCNGG 5 cut(s) 102, 429, 918, 1005, 1220
BmsI GCATC 2 cut(s) 26, 175
BplI GAGNNNNNCTC 2 cut(s) 1066, 1098
Bpu10I CCTNAGC 2 cut(s) 546, 1145
Bpu1102I GCTNAGC 1 cut(s) 15
BpuEI CTTGAG 5 cut(s) 146, 152, 508, 914, 1068
BpuMI CCSGG 3 cut(s) 429, 1005, 1220
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 6 cut(s) 126, 213, 603, 750, 793, 1218
BsaXI ACNNNNNCTCC 4 cut(s) 321, 351, 1121, 1151
Bsc4I CCNNNNNNNGG 7 cut(s) 57, 218, 520, 547, 1004, 1133, 1193
Bse1I ACTGG 2 cut(s) 505, 1187
BseBI CCWGG 2 cut(s) 102, 918
BseDI CCNNGG 6 cut(s) 126, 213, 603, 750, 793, 1218
BseGI GGATG 3 cut(s) 190, 501, 556
BseLI CCNNNNNNNGG 7 cut(s) 57, 218, 520, 547, 1004, 1133, 1193
BseMII CTCAG 5 cut(s) 29, 270, 537, 748, 1159
BseNI ACTGG 2 cut(s) 505, 1187
BseRI GAGGAG 2 cut(s) 455, 1064
Bsh1236I CGCG 1 cut(s) 128
BshFI GGCC 5 cut(s) 602, 615, 749, 855, 966
BshNI GGYRCC 1 cut(s) 824
BsiHKCI CYCGRG 2 cut(s) 55, 257
BsiSI CCGG 5 cut(s) 429, 1005, 1069, 1127, 1220
BslI CCNNNNNNNGG 7 cut(s) 57, 218, 520, 547, 1004, 1133, 1193
BsmAI GTCTC 4 cut(s) 12, 124, 229, 895
BsmBI CGTCTC 2 cut(s) 12, 124
BsmI GAATGC 2 cut(s) 145, 387
BsnI GGCC 5 cut(s) 602, 615, 749, 855, 966
BsoBI CYCGRG 2 cut(s) 55, 257
Bsp143I GATC 3 cut(s) 483, 667, 873
Bsp1720I GCTNAGC 1 cut(s) 15
Bsp19I CCATGG 1 cut(s) 213
BspACI CCGC 8 cut(s) 44, 123, 126, 128, 742, 1046, 1088, 1106
BspANI GGCC 5 cut(s) 602, 615, 749, 855, 966
BspCNI CTCAG 5 cut(s) 28, 271, 538, 747, 1158
BspFNI CGCG 1 cut(s) 128
BspHI TCATGA 2 cut(s) 880, 936
BspLI GGNNCC 2 cut(s) 826, 1167
BspT107I GGYRCC 1 cut(s) 824
BspTI CTTAAG 1 cut(s) 31
BsrBI CCGCTC 1 cut(s) 46
BsrI ACTGG 2 cut(s) 505, 1187
BssECI CCNNGG 6 cut(s) 126, 213, 603, 750, 793, 1218
BssMI GATC 3 cut(s) 483, 667, 873
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 1101
BssT1I CCWWGG 2 cut(s) 213, 793
Bst2BI CACGAG 1 cut(s) 1101
Bst2UI CCWGG 2 cut(s) 102, 918
Bst4CI ACNGT 6 cut(s) 198, 307, 720, 802, 907, 956
Bst6I CTCTTC 1 cut(s) 372
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 31
BstC8I GCNNGC 1 cut(s) 385
BstDEI CTNAG 7 cut(s) 15, 279, 291, 546, 617, 734, 1145
BstDSI CCRYGG 4 cut(s) 126, 213, 603, 750
BstF5I GGATG 3 cut(s) 190, 501, 556
BstFNI CGCG 1 cut(s) 128
BstH2I RGCGCY 1 cut(s) 160
BstHHI GCGC 1 cut(s) 159
BstKTI GATC 3 cut(s) 486, 670, 876
BstMAI GTCTC 4 cut(s) 12, 124, 229, 895
BstMBI GATC 3 cut(s) 483, 667, 873
BstMWI GCNNNNNNNGC 5 cut(s) 11, 36, 67, 106, 163
BstNI CCWGG 2 cut(s) 102, 918
BstNSI RCATGY 2 cut(s) 387, 1114
BstSCI CCNGG 5 cut(s) 100, 427, 916, 1003, 1218
BstUI CGCG 1 cut(s) 128
BstX2I RGATCY 1 cut(s) 667
BstYI RGATCY 1 cut(s) 667
BsuRI GGCC 5 cut(s) 602, 615, 749, 855, 966
BtgI CCRYGG 4 cut(s) 126, 213, 603, 750
BtsCI GGATG 3 cut(s) 190, 501, 556
Cac8I GCNNGC 1 cut(s) 385
CciI TCATGA 2 cut(s) 880, 936
CfoI GCGC 1 cut(s) 159
Cfr13I GGNCC 1 cut(s) 1166
Cfr42I CCGCGG 1 cut(s) 129
CsiI ACCWGGT 1 cut(s) 916
Csp6I GTAC 3 cut(s) 204, 265, 798
CviAII CATG 9 cut(s) 214, 384, 519, 863, 881, 937, 950, 1012, 1111
CviQI GTAC 3 cut(s) 204, 265, 798
DdeI CTNAG 7 cut(s) 15, 279, 291, 546, 617, 734, 1145
DpnI GATC 3 cut(s) 485, 669, 875
DpnII GATC 3 cut(s) 483, 667, 873
DrdI GACNNNNNNGTC 1 cut(s) 1187
DseDI GACNNNNNNGTC 1 cut(s) 1187
EaeI YGGCCR 1 cut(s) 600
Eam1104I CTCTTC 1 cut(s) 372
EarI CTCTTC 1 cut(s) 372
EciI GGCGGA 2 cut(s) 112, 1061
Eco130I CCWWGG 2 cut(s) 213, 793
Eco147I AGGCCT 1 cut(s) 615
Eco32I GATATC 1 cut(s) 645
Eco47I GGWCC 1 cut(s) 1166
Eco57I CTGAAG 2 cut(s) 342, 713
Eco88I CYCGRG 2 cut(s) 55, 257
EcoO109I RGGNCCY 1 cut(s) 1166
EcoRII CCWGG 2 cut(s) 100, 916
EcoRV GATATC 1 cut(s) 645
EcoT14I CCWWGG 2 cut(s) 213, 793
EcoT22I ATGCAT 1 cut(s) 389
ErhI CCWWGG 2 cut(s) 213, 793
Esp3I CGTCTC 2 cut(s) 12, 124
FaeI CATG 9 cut(s) 217, 387, 522, 866, 884, 940, 953, 1015, 1114
FalI AAGNNNNNCTT 8 cut(s) 161, 193, 254, 286, 657, 689, 756, 788
FatI CATG 9 cut(s) 213, 383, 518, 862, 880, 936, 949, 1011, 1110
FauNDI CATATG 1 cut(s) 820
FblI GTMKAC 1 cut(s) 532
Fnu4HI GCNGC 1 cut(s) 126
FokI GGATG 3 cut(s) 197, 488, 563
Fsp4HI GCNGC 1 cut(s) 126
FspBI CTAG 2 cut(s) 515, 723
GlaI GCGC 1 cut(s) 158
GluI GCNGC 1 cut(s) 126
HaeII RGCGCY 1 cut(s) 160
HaeIII GGCC 5 cut(s) 602, 615, 749, 855, 966
HapII CCGG 5 cut(s) 429, 1005, 1069, 1127, 1220
HhaI GCGC 1 cut(s) 159
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 9 cut(s) 217, 387, 522, 866, 884, 940, 953, 1015, 1114
Hin6I GCGC 1 cut(s) 157
HinP1I GCGC 1 cut(s) 157
HincII GTYRAC 2 cut(s) 340, 1140
HindII GTYRAC 2 cut(s) 340, 1140
HindIII AAGCTT 1 cut(s) 1028
HinfI GANTC 6 cut(s) 239, 685, 846, 888, 1015, 1158
HpaI GTTAAC 1 cut(s) 1140
HpaII CCGG 5 cut(s) 429, 1005, 1069, 1127, 1220
HphI GGTGA 5 cut(s) 33, 307, 470, 932, 1019
Hpy166II GTNNAC 3 cut(s) 340, 533, 1140
Hpy188I TCNGA 7 cut(s) 280, 693, 807, 873, 895, 1153, 1180
Hpy8I GTNNAC 3 cut(s) 340, 533, 1140
Hpy99I CGWCG 2 cut(s) 1157, 1214
HpyAV CCTTC 4 cut(s) 212, 605, 866, 1036
HpyCH4III ACNGT 6 cut(s) 198, 307, 720, 802, 907, 956
HpyCH4IV ACGT 1 cut(s) 1212
HpyCH4V TGCA 3 cut(s) 143, 350, 387
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 36, 67, 106, 163
HpyF3I CTNAG 7 cut(s) 15, 279, 291, 546, 617, 734, 1145
HpySE526I ACGT 1 cut(s) 1212
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 9 cut(s) 217, 387, 522, 866, 884, 940, 953, 1015, 1114
HspAI GCGC 1 cut(s) 157
KspAI GTTAAC 1 cut(s) 1140
KspI CCGCGG 1 cut(s) 129
Kzo9I GATC 3 cut(s) 483, 667, 873
LmnI GCTCC 5 cut(s) 114, 329, 543, 1119, 1154
LweI GCATC 2 cut(s) 26, 175
MabI ACCWGGT 1 cut(s) 916
MaeI CTAG 2 cut(s) 515, 723
MaeII ACGT 1 cut(s) 1212
MaeIII GTNAC 4 cut(s) 737, 920, 1007, 1228
MalI GATC 3 cut(s) 485, 669, 875
MbiI CCGCTC 1 cut(s) 46
MboI GATC 3 cut(s) 483, 667, 873
MboII GAAGA 8 cut(s) 169, 348, 389, 422, 662, 677, 725, 851
MflI RGATCY 1 cut(s) 667
MluCI AATT 4 cut(s) 20, 274, 777, 1077
MlyI GAGTC 2 cut(s) 233, 897
MmeI TCCRAC 2 cut(s) 937, 1176
Mph1103I ATGCAT 1 cut(s) 389
MroXI GAANNNNTTC 1 cut(s) 669
MseI TTAA 3 cut(s) 32, 494, 1139
MslI CAYNNNNRTG 1 cut(s) 954
MspA1I CMGCKG 1 cut(s) 128
MspCI CTTAAG 1 cut(s) 31
MspI CCGG 5 cut(s) 429, 1005, 1069, 1127, 1220
MspR9I CCNGG 5 cut(s) 102, 429, 918, 1005, 1220
Mva1269I GAATGC 2 cut(s) 145, 387
MvaI CCWGG 2 cut(s) 102, 918
MvnI CGCG 1 cut(s) 128
MwoI GCNNNNNNNGC 5 cut(s) 11, 36, 67, 106, 163
NciI CCSGG 3 cut(s) 429, 1005, 1220
NcoI CCATGG 1 cut(s) 213
NdeI CATATG 1 cut(s) 820
NdeII GATC 3 cut(s) 483, 667, 873
NlaIII CATG 9 cut(s) 217, 387, 522, 866, 884, 940, 953, 1015, 1114
NlaIV GGNNCC 2 cut(s) 826, 1167
NmuCI GTSAC 3 cut(s) 920, 1007, 1228
NsiI ATGCAT 1 cut(s) 389
NspI RCATGY 2 cut(s) 387, 1114
PaeI GCATGC 1 cut(s) 387
PaeR7I CTCGAG 2 cut(s) 55, 257
PagI TCATGA 2 cut(s) 880, 936
PceI AGGCCT 1 cut(s) 615
PcsI WCGNNNNNNNCGW 3 cut(s) 62, 264, 471
PctI GAATGC 2 cut(s) 145, 387
PdmI GAANNNNTTC 1 cut(s) 669
PfeI GAWTC 4 cut(s) 685, 846, 1015, 1158
PflFI GACNNNGTC 1 cut(s) 887
PfoI TCCNGGA 1 cut(s) 427
PkrI GCNGC 1 cut(s) 127
PleI GAGTC 2 cut(s) 233, 896
PpsI GAGTC 2 cut(s) 233, 896
PpuMI RGGWCCY 1 cut(s) 1166
Psp5II RGGWCCY 1 cut(s) 1166
Psp6I CCWGG 2 cut(s) 100, 916
PspGI CCWGG 2 cut(s) 100, 916
PspN4I GGNNCC 2 cut(s) 826, 1167
PspPI GGNCC 1 cut(s) 1166
PspPPI RGGWCCY 1 cut(s) 1166
PspXI VCTCGAGB 1 cut(s) 55
PsuI RGATCY 1 cut(s) 667
PsyI GACNNNGTC 1 cut(s) 887
RsaI GTAC 3 cut(s) 205, 266, 799
RsaNI GTAC 3 cut(s) 204, 265, 798
RseI CAYNNNNRTG 1 cut(s) 954
SacII CCGCGG 1 cut(s) 129
SaqAI TTAA 3 cut(s) 32, 494, 1139
SatI GCNGC 1 cut(s) 126
Sau3AI GATC 3 cut(s) 483, 667, 873
Sau96I GGNCC 1 cut(s) 1166
SchI GAGTC 2 cut(s) 233, 897
ScrFI CCNGG 5 cut(s) 102, 429, 918, 1005, 1220
SexAI ACCWGGT 1 cut(s) 916
SfaNI GCATC 2 cut(s) 26, 175
Sfr274I CTCGAG 2 cut(s) 55, 257
Sfr303I CCGCGG 1 cut(s) 129
SgrBI CCGCGG 1 cut(s) 129
SinI GGWCC 1 cut(s) 1166
SlaI CTCGAG 2 cut(s) 55, 257
SmiMI CAYNNNNRTG 1 cut(s) 954
SmlI CTYRAG 8 cut(s) 31, 55, 161, 167, 257, 487, 929, 1083
SmoI CTYRAG 8 cut(s) 31, 55, 161, 167, 257, 487, 929, 1083
SphI GCATGC 1 cut(s) 387
Sse9I AATT 4 cut(s) 20, 274, 777, 1077
SseBI AGGCCT 1 cut(s) 615
SsiI CCGC 8 cut(s) 44, 123, 126, 128, 742, 1046, 1088, 1106
SspMI CTAG 2 cut(s) 515, 723
StuI AGGCCT 1 cut(s) 615
StyD4I CCNGG 5 cut(s) 100, 427, 916, 1003, 1218
StyI CCWWGG 2 cut(s) 213, 793
TaaI ACNGT 6 cut(s) 198, 307, 720, 802, 907, 956
TaiI ACGT 1 cut(s) 1215
TaqI TCGA 7 cut(s) 56, 65, 137, 180, 258, 834, 1191
TaqII GACCGA 1 cut(s) 900
TasI AATT 4 cut(s) 20, 274, 777, 1077
TauI GCSGC 1 cut(s) 128
TfiI GAWTC 4 cut(s) 685, 846, 1015, 1158
Tru1I TTAA 3 cut(s) 32, 494, 1139
Tru9I TTAA 3 cut(s) 32, 494, 1139
TseFI GTSAC 3 cut(s) 920, 1007, 1228
Tsp45I GTSAC 3 cut(s) 920, 1007, 1228
TspDTI ATGAA 2 cut(s) 726, 851
TspGWI ACGGA 2 cut(s) 1106, 1170
Tth111I GACNNNGTC 1 cut(s) 887
Vha464I CTTAAG 1 cut(s) 31
VpaK11BI GGWCC 1 cut(s) 1166
XceI RCATGY 2 cut(s) 387, 1114
XcmI CCANNNNNNNNNTGG 2 cut(s) 770, 1130
XhoI CTCGAG 2 cut(s) 55, 257
XmiI GTMKAC 1 cut(s) 532
XmnI GAANNNNTTC 1 cut(s) 669
XspI CTAG 2 cut(s) 515, 723
Zsp2I ATGCAT 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.