FvH4_4g22000

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016311
Physical Location & Seq
Forward (+)
24936971 .. 24939009
2039 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g22000.t3

Sequence Viewer

Length: 1086 bp
ATGAATATGATAGCTGAATTAGTATTGACGGTAGTCTATGTTATAGCAACAGCACTGATACCGCTTGTGAGCTTAAGCCTTGTCAAGGGATGTTCTTGGACATGGTCATGGACCTCATGCTCATGGACATGGTCGAGTTCATGGCGCCTACCGTTTCCGACTCTCTCCCTTCATGATTCTGCTCTGGGAAGACTGCTCTCTTGCTTCAATAGCCACAGAAAGAGAATAATATCGGAGTTAGTCAGCGACACTTATCCTTCTTCTGATCAGGAACAAGAACCTAGTGAGTTGAATGAAGAAGATTTGAGAGTATTGATTTCTCATATTGAGGAGACTGATGGCGGTCCACCTTGGAAACTCATGATGGAGCAGTCTATTCCTTCATTGACATACAAAGCTTGGTACCGTGATCCACCGACAGGCCCCCCTCAGTACCGTACTAGAACAGTTTTTGAAAACGTATCACCGGAGATATTGAGGGATTTCTTCTGGGATGATGAATTTAGACCCAGGTGGGATAAGATGCTCATTCACTCCAAGGTTCTAAGCACTTGCCTCCAAACTGGTACCATGATTGTCCATTGGATTAGGAAGCTACCACTCTTCTGCAGTGCTCGAGAATATATCATCATCCGTCGCATTTGGGAGTCTGCAACAGGATATTATTGTGTAACAAAGGGAACACCATATCCATCTCTACCAAGGCTAAGAAGACCGAAACGAGTAGATGTGTATCATTCAAGTTGGTGTATAAGGCCAGTGAAATCCAGGAATGCAGATCAGCAAACAGGATCAGAAGTGCTTCTGTTTCACTATGAAGACATAGGTCTTCCAAAAGAGGTTGTCAAAATGGGCGTCAGGGCAGGAATGTGGAACATGGTAAAGAAACTACATCTGGGAGTTCAGATCTACAATATGGAAAAGGCAAATGATGAATCCCCTTCATACTATGCAATTATGGCACACATTACAACAAAGCTTCCGGCTTCACCATCGACAATCATGTATCACATTCCTCAAGTTGAAGCGGATGAAAATGCATGCCAGAACCAAAACCCAGAAGCACAAGCAATAGAGAGTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

362

Amino Acids

41.66

Weight (kDa)

6.8

Isoelectric Point (pI)

57.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016311)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g22000 FvH4_4g22000 FvH4_4g22000
malus_domestica MD13G1135100.v1.1
prunus_persica Prupe.1G210200_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0428201
rosa_laevigata RLG00000007170
rosa_multiflora Rmu_sc0004940.1_g000004 Rmu_sc0009599.1_g000003
rosa_rugosa Rorug04G0223600
rosa_samantha Rh4AG280800 Rh4BG286300 Rh4CG302000 Rh4DG283300
rosa_wichuraiana Rw4G024390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 402, 566
AccB1I GGYRCC 3 cut(s) 144, 402, 566
AciI CCGC 3 cut(s) 62, 342, 1028
AclWI GGATC 2 cut(s) 404, 799
AcsI RAATTY 1 cut(s) 500
AcyI GRCGYC 2 cut(s) 145, 855
AfaI GTAC 4 cut(s) 404, 434, 439, 568
AfiI CCNNNNNNNGG 2 cut(s) 85, 419
AflII CTTAAG 1 cut(s) 73
AgsI TTSAA 5 cut(s) 208, 292, 455, 741, 1025
AjnI CCWGG 2 cut(s) 509, 767
AloI GAACNNNNNNTCC 2 cut(s) 673, 705
AluBI AGCT 5 cut(s) 14, 72, 398, 595, 979
AluI AGCT 5 cut(s) 14, 72, 398, 595, 979
Alw21I GWGCWC 1 cut(s) 616
Alw26I GTCTC 1 cut(s) 326
AlwI GGATC 2 cut(s) 404, 799
Ama87I CYCGRG 1 cut(s) 615
AoxI GGCC 2 cut(s) 421, 755
ApoI RAATTY 1 cut(s) 500
ArsI GACNNNNNNTTYG 2 cut(s) 840, 872
Asp700I GAANNNNTTC 1 cut(s) 801
Asp718I GGTACC 2 cut(s) 402, 566
AspLEI GCGC 1 cut(s) 147
AspS9I GGNCC 3 cut(s) 111, 344, 422
AsuHPI GGTGA 2 cut(s) 456, 981
AvaI CYCGRG 1 cut(s) 615
AvaII GGWCC 2 cut(s) 111, 344
BanI GGYRCC 3 cut(s) 144, 402, 566
BbsI GAAGAC 4 cut(s) 196, 718, 821, 825
Bbv12I GWGCWC 1 cut(s) 616
BccI CCATC 4 cut(s) 332, 358, 700, 1000
BciT130I CCWGG 2 cut(s) 511, 769
BclI TGATCA 1 cut(s) 265
BcoDI GTCTC 1 cut(s) 326
BfaI CTAG 2 cut(s) 282, 441
BfmI CTRYAG 1 cut(s) 607
BfoI RGCGCY 1 cut(s) 148
BfrI CTTAAG 1 cut(s) 73
BglII AGATCT 1 cut(s) 906
Bme1390I CCNGG 2 cut(s) 511, 769
Bme18I GGWCC 2 cut(s) 111, 344
BmeT110I CYCGRG 1 cut(s) 615
BmgT120I GGNCC 3 cut(s) 111, 344, 422
BmiI GGNNCC 4 cut(s) 146, 404, 424, 568
BmrFI CCNGG 2 cut(s) 511, 769
BmsI GCATC 1 cut(s) 513
BoxI GACNNNNGTC 2 cut(s) 32, 825
BpiI GAAGAC 4 cut(s) 196, 718, 821, 825
BpuEI CTTGAG 1 cut(s) 1002
BsaBI GATNNNNATC 1 cut(s) 732
BsaHI GRCGYC 2 cut(s) 145, 855
BsaJI CCNNGG 4 cut(s) 350, 509, 537, 701
BsaWI WCCGGW 1 cut(s) 466
Bsc4I CCNNNNNNNGG 2 cut(s) 85, 419
Bse1I ACTGG 2 cut(s) 568, 758
Bse8I GATNNNNATC 1 cut(s) 732
BseBI CCWGG 2 cut(s) 511, 769
BseDI CCNNGG 4 cut(s) 350, 509, 537, 701
BseGI GGATG 4 cut(s) 95, 499, 630, 1036
BseJI GATNNNNATC 1 cut(s) 732
BseLI CCNNNNNNNGG 2 cut(s) 85, 419
BseMII CTCAG 1 cut(s) 443
BseNI ACTGG 2 cut(s) 568, 758
BseRI GAGGAG 1 cut(s) 344
BshFI GGCC 2 cut(s) 423, 757
BshNI GGYRCC 3 cut(s) 144, 402, 566
BsiHKAI GWGCWC 1 cut(s) 616
BsiHKCI CYCGRG 1 cut(s) 615
BsiSI CCGG 2 cut(s) 467, 983
BslI CCNNNNNNNGG 2 cut(s) 85, 419
BsmAI GTCTC 1 cut(s) 326
BsmI GAATGC 1 cut(s) 778
BsnI GGCC 2 cut(s) 423, 757
BsoBI CYCGRG 1 cut(s) 615
Bsp1286I GDGCHC 1 cut(s) 616
Bsp143I GATC 5 cut(s) 265, 409, 778, 791, 906
BspACI CCGC 3 cut(s) 62, 342, 1028
BspANI GGCC 2 cut(s) 423, 757
BspCNI CTCAG 1 cut(s) 442
BspHI TCATGA 2 cut(s) 172, 360
BspLI GGNNCC 4 cut(s) 146, 404, 424, 568
BspMAI CTGCAG 1 cut(s) 611
BspPI GGATC 2 cut(s) 404, 799
BspT107I GGYRCC 3 cut(s) 144, 402, 566
BspTI CTTAAG 1 cut(s) 73
BsrI ACTGG 2 cut(s) 568, 758
BssECI CCNNGG 4 cut(s) 350, 509, 537, 701
BssMI GATC 5 cut(s) 265, 409, 778, 791, 906
BssNI GRCGYC 2 cut(s) 145, 855
BssT1I CCWWGG 3 cut(s) 350, 537, 701
Bst2UI CCWGG 2 cut(s) 511, 769
Bst4CI ACNGT 5 cut(s) 31, 153, 407, 437, 448
Bst6I CTCTTC 1 cut(s) 608
BstACI GRCGYC 2 cut(s) 145, 855
BstAFI CTTAAG 1 cut(s) 73
BstC8I GCNNGC 1 cut(s) 1042
BstDEI CTNAG 3 cut(s) 429, 545, 707
BstENI CCTNNNNNAGG 1 cut(s) 83
BstF5I GGATG 4 cut(s) 95, 499, 630, 1036
BstH2I RGCGCY 1 cut(s) 148
BstHHI GCGC 1 cut(s) 147
BstKTI GATC 5 cut(s) 268, 412, 781, 794, 909
BstMAI GTCTC 1 cut(s) 326
BstMBI GATC 5 cut(s) 265, 409, 778, 791, 906
BstMWI GCNNNNNNNGC 2 cut(s) 210, 959
BstNI CCWGG 2 cut(s) 511, 769
BstNSI RCATGY 1 cut(s) 1044
BstPAI GACNNNNGTC 2 cut(s) 32, 825
BstSCI CCNGG 2 cut(s) 509, 767
BstSFI CTRYAG 1 cut(s) 607
BstV2I GAAGAC 4 cut(s) 196, 718, 821, 825
BstX2I RGATCY 1 cut(s) 906
BstYI RGATCY 1 cut(s) 906
BsuRI GGCC 2 cut(s) 423, 757
BtsCI GGATG 4 cut(s) 95, 499, 630, 1036
BtsI GCAGTG 1 cut(s) 616
BtsIMutI CAGTG 3 cut(s) 53, 616, 765
Cac8I GCNNGC 1 cut(s) 1042
CciI TCATGA 2 cut(s) 172, 360
CfoI GCGC 1 cut(s) 147
Cfr13I GGNCC 3 cut(s) 111, 344, 422
CseI GACGC 1 cut(s) 844
Csp6I GTAC 4 cut(s) 403, 433, 438, 567
CviQI GTAC 4 cut(s) 403, 433, 438, 567
DdeI CTNAG 3 cut(s) 429, 545, 707
DinI GGCGCC 1 cut(s) 146
DpnI GATC 5 cut(s) 267, 411, 780, 793, 908
DpnII GATC 5 cut(s) 265, 409, 778, 791, 906
Eam1104I CTCTTC 1 cut(s) 608
EarI CTCTTC 1 cut(s) 608
Eco130I CCWWGG 3 cut(s) 350, 537, 701
Eco47I GGWCC 2 cut(s) 111, 344
Eco88I CYCGRG 1 cut(s) 615
EcoNI CCTNNNNNAGG 1 cut(s) 83
EcoO109I RGGNCCY 1 cut(s) 422
EcoRII CCWGG 2 cut(s) 509, 767
EcoT14I CCWWGG 3 cut(s) 350, 537, 701
EcoT22I ATGCAT 1 cut(s) 1042
EgeI GGCGCC 1 cut(s) 146
EheI GGCGCC 1 cut(s) 146
ErhI CCWWGG 3 cut(s) 350, 537, 701
FbaI TGATCA 1 cut(s) 265
FokI GGATG 4 cut(s) 102, 506, 617, 1043
FspBI CTAG 2 cut(s) 282, 441
GlaI GCGC 1 cut(s) 146
HaeII RGCGCY 1 cut(s) 148
HaeIII GGCC 2 cut(s) 423, 757
HapII CCGG 2 cut(s) 467, 983
HgaI GACGC 1 cut(s) 844
HhaI GCGC 1 cut(s) 147
Hin1I GRCGYC 2 cut(s) 145, 855
Hin6I GCGC 1 cut(s) 145
HinP1I GCGC 1 cut(s) 145
HindIII AAGCTT 2 cut(s) 396, 977
HinfI GANTC 4 cut(s) 160, 176, 647, 935
HpaII CCGG 2 cut(s) 467, 983
HphI GGTGA 2 cut(s) 456, 981
Hpy166II GTNNAC 1 cut(s) 347
Hpy188I TCNGA 5 cut(s) 159, 235, 265, 796, 906
Hpy188III TCNNGA 4 cut(s) 173, 269, 361, 617
Hpy8I GTNNAC 1 cut(s) 347
Hpy99I CGWCG 1 cut(s) 639
HpyAV CCTTC 4 cut(s) 179, 267, 390, 951
HpyCH4III ACNGT 5 cut(s) 31, 153, 407, 437, 448
HpyCH4IV ACGT 1 cut(s) 459
HpyCH4V TGCA 5 cut(s) 609, 653, 776, 953, 1040
HpyF10VI GCNNNNNNNGC 2 cut(s) 210, 959
HpyF3I CTNAG 3 cut(s) 429, 545, 707
HpySE526I ACGT 1 cut(s) 459
Hsp92I GRCGYC 2 cut(s) 145, 855
HspAI GCGC 1 cut(s) 145
KasI GGCGCC 1 cut(s) 144
KpnI GGTACC 2 cut(s) 406, 570
Ksp22I TGATCA 1 cut(s) 265
Kzo9I GATC 5 cut(s) 265, 409, 778, 791, 906
LmnI GCTCC 1 cut(s) 367
LweI GCATC 1 cut(s) 513
MaeI CTAG 2 cut(s) 282, 441
MaeII ACGT 1 cut(s) 459
MaeIII GTNAC 1 cut(s) 670
MalI GATC 5 cut(s) 267, 411, 780, 793, 908
MboI GATC 5 cut(s) 265, 409, 778, 791, 906
MboII GAAGA 9 cut(s) 201, 252, 308, 311, 478, 595, 723, 821, 830
MflI RGATCY 1 cut(s) 906
MhlI GDGCHC 1 cut(s) 616
MluCI AATT 3 cut(s) 17, 500, 954
Mly113I GGCGCC 1 cut(s) 145
MlyI GAGTC 2 cut(s) 154, 656
MmeI TCCRAC 1 cut(s) 182
MnlI CCTC 7 cut(s) 124, 322, 438, 471, 566, 832, 1026
Mph1103I ATGCAT 1 cut(s) 1042
MroXI GAANNNNTTC 1 cut(s) 801
MseI TTAA 1 cut(s) 74
MslI CAYNNNNRTG 3 cut(s) 106, 121, 127
MspCI CTTAAG 1 cut(s) 73
MspI CCGG 2 cut(s) 467, 983
MspR9I CCNGG 2 cut(s) 511, 769
Mva1269I GAATGC 1 cut(s) 778
MvaI CCWGG 2 cut(s) 511, 769
MwoI GCNNNNNNNGC 2 cut(s) 210, 959
NarI GGCGCC 1 cut(s) 145
NdeII GATC 5 cut(s) 265, 409, 778, 791, 906
NlaIV GGNNCC 4 cut(s) 146, 404, 424, 568
NsiI ATGCAT 1 cut(s) 1042
NspI RCATGY 1 cut(s) 1044
PaeI GCATGC 1 cut(s) 1044
PaeR7I CTCGAG 1 cut(s) 615
PagI TCATGA 2 cut(s) 172, 360
PctI GAATGC 1 cut(s) 778
PdmI GAANNNNTTC 1 cut(s) 801
PfeI GAWTC 2 cut(s) 176, 935
PflFI GACNNNGTC 2 cut(s) 103, 130
PfoI TCCNGGA 1 cut(s) 767
PleI GAGTC 2 cut(s) 154, 655
PluTI GGCGCC 1 cut(s) 148
PpsI GAGTC 2 cut(s) 154, 655
PshAI GACNNNNGTC 2 cut(s) 32, 825
Psp6I CCWGG 2 cut(s) 509, 767
PspGI CCWGG 2 cut(s) 509, 767
PspN4I GGNNCC 4 cut(s) 146, 404, 424, 568
PspPI GGNCC 3 cut(s) 111, 344, 422
PstI CTGCAG 1 cut(s) 611
PsuI RGATCY 1 cut(s) 906
PsyI GACNNNGTC 2 cut(s) 103, 130
RsaI GTAC 4 cut(s) 404, 434, 439, 568
RsaNI GTAC 4 cut(s) 403, 433, 438, 567
RseI CAYNNNNRTG 3 cut(s) 106, 121, 127
SaqAI TTAA 1 cut(s) 74
Sau3AI GATC 5 cut(s) 265, 409, 778, 791, 906
Sau96I GGNCC 3 cut(s) 111, 344, 422
SchI GAGTC 2 cut(s) 154, 656
ScrFI CCNGG 2 cut(s) 511, 769
SduI GDGCHC 1 cut(s) 616
SfaNI GCATC 1 cut(s) 513
SfcI CTRYAG 1 cut(s) 607
SfoI GGCGCC 1 cut(s) 146
Sfr274I CTCGAG 1 cut(s) 615
SinI GGWCC 2 cut(s) 111, 344
SlaI CTCGAG 1 cut(s) 615
SmiMI CAYNNNNRTG 3 cut(s) 106, 121, 127
SmlI CTYRAG 3 cut(s) 73, 615, 1017
SmoI CTYRAG 3 cut(s) 73, 615, 1017
SphI GCATGC 1 cut(s) 1044
Sse9I AATT 3 cut(s) 17, 500, 954
SsiI CCGC 3 cut(s) 62, 342, 1028
SspDI GGCGCC 1 cut(s) 144
SspMI CTAG 2 cut(s) 282, 441
StyD4I CCNGG 2 cut(s) 509, 767
StyI CCWWGG 3 cut(s) 350, 537, 701
TaaI ACNGT 5 cut(s) 31, 153, 407, 437, 448
TaiI ACGT 1 cut(s) 462
TaqI TCGA 3 cut(s) 134, 616, 995
TaqII GACCGA 1 cut(s) 730
TasI AATT 3 cut(s) 17, 500, 954
TfiI GAWTC 2 cut(s) 176, 935
Tru1I TTAA 1 cut(s) 74
Tru9I TTAA 1 cut(s) 74
TscAI CASTG 3 cut(s) 60, 616, 765
TspGWI ACGGA 1 cut(s) 623
TspRI CASTG 3 cut(s) 60, 616, 765
Tth111I GACNNNGTC 2 cut(s) 103, 130
Vha464I CTTAAG 1 cut(s) 73
VpaK11BI GGWCC 2 cut(s) 111, 344
XagI CCTNNNNNAGG 1 cut(s) 83
XapI RAATTY 1 cut(s) 500
XceI RCATGY 1 cut(s) 1044
XhoI CTCGAG 1 cut(s) 615
XmnI GAANNNNTTC 1 cut(s) 801
XspI CTAG 2 cut(s) 282, 441
Zsp2I ATGCAT 1 cut(s) 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.