Rh4BG286300

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0016311
Physical Location & Seq
Forward (+)
45972324 .. 45973908
1585 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG286300.1

Sequence Viewer

Length: 606 bp
ATGAATATGATAGCCGAATTACTATTGACGGTAGTCTGTTTTGTAGCAGCAGCACTGATACCACTTGAACAAGAACCTTGTGAAGTGACTGAAGAAGATTTGAAAGTATTGGTCTCTCATATTGAGGAGACTGATGGCGGTCCACCTTGGAATCTCATGATGGAGCAGTCTATTCCTTCCTTGACATACAAAGCTTGGTATCGCGATCCACCGTTTCTGTTCACTAGTAGAAGTCTATTGCGCTCAAGCATTACATCGAAAACCTATCTTTCAGTGAAACCCAGGAATGCAGATCAGCAAATGGGATCAGAAGTGCTTCTATTTCACTACGAAGACATAGGTCTCCCAAGAGAGGTTGTCAAAATGGGCGTCAGGGCAGGAATGTGGAACATGGTAAAGAAACTACATTTAGGAGTTCAGGTTTACAGTATGGAAAAAGCAAATGATGAATCTCCTTCATACCATGCACTTATGGCACACATAACTACAAAGCTTCCGGCGTCACCATCCACAATCATGTATCACATCCCAGAAGTTGAAGCAGATGAGAATGCATGCCAGAGCCAAAACCCACAAGAACAAGTAACAGAGTTCATGGTTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

201

Amino Acids

22.75

Weight (kDa)

4.94

Isoelectric Point (pI)

63.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016311)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g22000 FvH4_4g22000 FvH4_4g22000
malus_domestica MD13G1135100.v1.1
prunus_persica Prupe.1G210200_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0428201
rosa_laevigata RLG00000007170
rosa_multiflora Rmu_sc0004940.1_g000004 Rmu_sc0009599.1_g000003
rosa_rugosa Rorug04G0223600
rosa_samantha Rh4AG280800 Rh4BG286300 Rh4CG302000 Rh4DG283300
rosa_wichuraiana Rw4G024390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 204
AciI CCGC 1 cut(s) 138
AclWI GGATC 2 cut(s) 200, 313
AcuI CTGAAG 1 cut(s) 111
AcyI GRCGYC 2 cut(s) 369, 500
AfiI CCNNNNNNNGG 1 cut(s) 352
AgsI TTSAA 3 cut(s) 68, 103, 539
AhlI ACTAGT 1 cut(s) 224
AjnI CCWGG 1 cut(s) 281
AluBI AGCT 2 cut(s) 194, 493
AluI AGCT 2 cut(s) 194, 493
Alw26I GTCTC 3 cut(s) 118, 122, 347
AlwI GGATC 2 cut(s) 200, 313
ApeKI GCWGC 2 cut(s) 47, 50
ArsI GACNNNNNNTTYG 2 cut(s) 354, 386
Asp700I GAANNNNTTC 1 cut(s) 315
AspLEI GCGC 1 cut(s) 243
AspS9I GGNCC 1 cut(s) 140
AsuHPI GGTGA 1 cut(s) 495
AvaII GGWCC 1 cut(s) 140
BbsI GAAGAC 1 cut(s) 339
BbvI GCAGC 2 cut(s) 59, 62
BccI CCATC 3 cut(s) 128, 154, 514
BciT130I CCWGG 1 cut(s) 283
BcoDI GTCTC 3 cut(s) 118, 122, 347
BcuI ACTAGT 1 cut(s) 224
BfaI CTAG 1 cut(s) 225
BisI GCNGC 2 cut(s) 48, 51
BlsI GCNGC 2 cut(s) 49, 52
Bme1390I CCNGG 1 cut(s) 283
Bme18I GGWCC 1 cut(s) 140
BmgT120I GGNCC 1 cut(s) 140
BmrFI CCNGG 1 cut(s) 283
BoxI GACNNNNGTC 2 cut(s) 32, 339
BpiI GAAGAC 1 cut(s) 339
BpuEI CTTGAG 1 cut(s) 229
BsaHI GRCGYC 2 cut(s) 369, 500
BsaI GGTCTC 2 cut(s) 118, 347
BsaJI CCNNGG 2 cut(s) 146, 281
BsaXI ACNNNNNCTCC 2 cut(s) 405, 435
Bsc4I CCNNNNNNNGG 1 cut(s) 352
BseBI CCWGG 1 cut(s) 283
BseDI CCNNGG 2 cut(s) 146, 281
BseGI GGATG 2 cut(s) 506, 525
BseLI CCNNNNNNNGG 1 cut(s) 352
BseRI GAGGAG 1 cut(s) 140
BseXI GCAGC 2 cut(s) 59, 62
Bsh1236I CGCG 1 cut(s) 204
BsiSI CCGG 1 cut(s) 497
BslI CCNNNNNNNGG 1 cut(s) 352
BsmAI GTCTC 3 cut(s) 118, 122, 347
BsmI GAATGC 2 cut(s) 292, 556
Bso31I GGTCTC 2 cut(s) 118, 347
Bsp143I GATC 3 cut(s) 205, 292, 305
Bsp68I TCGCGA 1 cut(s) 204
BspACI CCGC 1 cut(s) 138
BspFNI CGCG 1 cut(s) 204
BspHI TCATGA 1 cut(s) 156
BspPI GGATC 2 cut(s) 200, 313
BspTNI GGTCTC 2 cut(s) 118, 347
BssECI CCNNGG 2 cut(s) 146, 281
BssMI GATC 3 cut(s) 205, 292, 305
BssNI GRCGYC 2 cut(s) 369, 500
BssT1I CCWWGG 1 cut(s) 146
Bst2UI CCWGG 1 cut(s) 283
Bst4CI ACNGT 3 cut(s) 31, 213, 428
BstACI GRCGYC 2 cut(s) 369, 500
BstC8I GCNNGC 1 cut(s) 556
BstF5I GGATG 2 cut(s) 506, 525
BstFNI CGCG 1 cut(s) 204
BstHHI GCGC 1 cut(s) 243
BstKTI GATC 3 cut(s) 208, 295, 308
BstMAI GTCTC 3 cut(s) 118, 122, 347
BstMBI GATC 3 cut(s) 205, 292, 305
BstMWI GCNNNNNNNGC 1 cut(s) 473
BstNI CCWGG 1 cut(s) 283
BstNSI RCATGY 1 cut(s) 558
BstPAI GACNNNNGTC 2 cut(s) 32, 339
BstSCI CCNGG 1 cut(s) 281
BstUI CGCG 1 cut(s) 204
BstV1I GCAGC 2 cut(s) 59, 62
BstV2I GAAGAC 1 cut(s) 339
BtsCI GGATG 2 cut(s) 506, 525
BtsIMutI CAGTG 2 cut(s) 53, 279
BtuMI TCGCGA 1 cut(s) 204
Cac8I GCNNGC 1 cut(s) 556
CciI TCATGA 1 cut(s) 156
CfoI GCGC 1 cut(s) 243
Cfr13I GGNCC 1 cut(s) 140
CseI GACGC 2 cut(s) 358, 489
CviAII CATG 6 cut(s) 157, 391, 464, 517, 555, 595
CviJI RGCY 4 cut(s) 14, 194, 493, 564
CviKI_1 RGCY 4 cut(s) 14, 194, 493, 564
DpnI GATC 3 cut(s) 207, 294, 307
DpnII GATC 3 cut(s) 205, 292, 305
Eco130I CCWWGG 1 cut(s) 146
Eco31I GGTCTC 2 cut(s) 118, 347
Eco47I GGWCC 1 cut(s) 140
Eco57I CTGAAG 1 cut(s) 111
EcoRII CCWGG 1 cut(s) 281
EcoT14I CCWWGG 1 cut(s) 146
EcoT22I ATGCAT 1 cut(s) 556
ErhI CCWWGG 1 cut(s) 146
FaeI CATG 6 cut(s) 160, 394, 467, 520, 558, 598
FatI CATG 6 cut(s) 156, 390, 463, 516, 554, 594
Fnu4HI GCNGC 2 cut(s) 48, 51
FokI GGATG 2 cut(s) 493, 512
Fsp4HI GCNGC 2 cut(s) 48, 51
FspBI CTAG 1 cut(s) 225
GlaI GCGC 1 cut(s) 242
GluI GCNGC 2 cut(s) 48, 51
HapII CCGG 1 cut(s) 497
HgaI GACGC 2 cut(s) 358, 489
HhaI GCGC 1 cut(s) 243
Hin1I GRCGYC 2 cut(s) 369, 500
Hin1II CATG 6 cut(s) 160, 394, 467, 520, 558, 598
Hin6I GCGC 1 cut(s) 241
HinP1I GCGC 1 cut(s) 241
HindIII AAGCTT 2 cut(s) 192, 491
HinfI GANTC 2 cut(s) 151, 449
HpaII CCGG 1 cut(s) 497
HphI GGTGA 1 cut(s) 495
Hpy166II GTNNAC 3 cut(s) 143, 222, 424
Hpy188I TCNGA 1 cut(s) 310
Hpy188III TCNNGA 2 cut(s) 157, 203
Hpy8I GTNNAC 3 cut(s) 143, 222, 424
HpyAV CCTTC 2 cut(s) 186, 465
HpyCH4III ACNGT 3 cut(s) 31, 213, 428
HpyCH4V TGCA 3 cut(s) 290, 467, 554
HpyF10VI GCNNNNNNNGC 1 cut(s) 473
Hsp92I GRCGYC 2 cut(s) 369, 500
Hsp92II CATG 6 cut(s) 160, 394, 467, 520, 558, 598
HspAI GCGC 1 cut(s) 241
Kzo9I GATC 3 cut(s) 205, 292, 305
LmnI GCTCC 1 cut(s) 163
LpnPI CCDG 8 cut(s) 268, 295, 358, 363, 404, 510, 543, 572
Lsp1109I GCAGC 2 cut(s) 59, 62
MaeI CTAG 1 cut(s) 225
MaeIII GTNAC 3 cut(s) 85, 501, 583
MalI GATC 3 cut(s) 207, 294, 307
MboI GATC 3 cut(s) 205, 292, 305
MboII GAAGA 3 cut(s) 104, 107, 344
MluCI AATT 1 cut(s) 17
MnlI CCTC 2 cut(s) 118, 346
Mph1103I ATGCAT 1 cut(s) 556
MroXI GAANNNNTTC 1 cut(s) 315
MslI CAYNNNNRTG 1 cut(s) 515
MspI CCGG 1 cut(s) 497
MspR9I CCNGG 1 cut(s) 283
Mva1269I GAATGC 2 cut(s) 292, 556
MvaI CCWGG 1 cut(s) 283
MvnI CGCG 1 cut(s) 204
MwoI GCNNNNNNNGC 1 cut(s) 473
NdeII GATC 3 cut(s) 205, 292, 305
NlaIII CATG 6 cut(s) 160, 394, 467, 520, 558, 598
NmuCI GTSAC 2 cut(s) 85, 501
NruI TCGCGA 1 cut(s) 204
NsiI ATGCAT 1 cut(s) 556
NspI RCATGY 1 cut(s) 558
PaeI GCATGC 1 cut(s) 558
PagI TCATGA 1 cut(s) 156
PctI GAATGC 2 cut(s) 292, 556
PdmI GAANNNNTTC 1 cut(s) 315
PfeI GAWTC 2 cut(s) 151, 449
PkrI GCNGC 2 cut(s) 49, 52
PshAI GACNNNNGTC 2 cut(s) 32, 339
Psp6I CCWGG 1 cut(s) 281
PspGI CCWGG 1 cut(s) 281
PspPI GGNCC 1 cut(s) 140
RruI TCGCGA 1 cut(s) 204
RseI CAYNNNNRTG 1 cut(s) 515
SatI GCNGC 2 cut(s) 48, 51
Sau3AI GATC 3 cut(s) 205, 292, 305
Sau96I GGNCC 1 cut(s) 140
ScrFI CCNGG 1 cut(s) 283
SetI ASST 8 cut(s) 79, 148, 196, 266, 343, 357, 423, 495
SinI GGWCC 1 cut(s) 140
SmiMI CAYNNNNRTG 1 cut(s) 515
SmlI CTYRAG 1 cut(s) 244
SmoI CTYRAG 1 cut(s) 244
SpeI ACTAGT 1 cut(s) 224
SphI GCATGC 1 cut(s) 558
Sse9I AATT 1 cut(s) 17
SsiI CCGC 1 cut(s) 138
SspMI CTAG 1 cut(s) 225
StyD4I CCNGG 1 cut(s) 281
StyI CCWWGG 1 cut(s) 146
TaaI ACNGT 3 cut(s) 31, 213, 428
TaqI TCGA 1 cut(s) 257
TasI AATT 1 cut(s) 17
TfiI GAWTC 2 cut(s) 151, 449
TscAI CASTG 2 cut(s) 60, 279
TseFI GTSAC 2 cut(s) 85, 501
TseI GCWGC 2 cut(s) 47, 50
Tsp45I GTSAC 2 cut(s) 85, 501
TspDTI ATGAA 4 cut(s) 17, 447, 462, 583
TspRI CASTG 2 cut(s) 60, 279
VpaK11BI GGWCC 1 cut(s) 140
XceI RCATGY 1 cut(s) 558
XmnI GAANNNNTTC 1 cut(s) 315
XspI CTAG 1 cut(s) 225
Zsp2I ATGCAT 1 cut(s) 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.