Rh4AG280800

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0016311
Physical Location & Seq
Forward (+)
59600689 .. 59602273
1585 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG280800.1

Sequence Viewer

Length: 852 bp
ATGAATATGATAGCCGAATTACTATTGACGGAACAAGAACCTTGTGAAGTGACTGAAGAAGATTTGAAAGTATTGGTCTCTCATATTGAGGAGACTGATGGCGGTCCACCTTGGAATCTCATGATGGAGCAGTCTATTCCTTCCTTGACATACAAAGCTTGGTATCGCGATCCACCGTTAGGCCCAACACAGTACCGTACTAAAACAGTTTTCGAAAATGTATCACCAGAGGTATTGAGAGATTTCTTCTGGGATGATGAATTCAGACCCAGGTGGGATAAAACGCTCATTCACACCAAGGTTCTAAGCGTTTGCCTCCAAACTGGTACCTTGATTGTTCATTGGATTAGAAAGCTACCACTATTCTGCAGCAATCGAGAATATATCATAATCCGTCGCATTTTTGAATCTGCATCAAGGTATTATTGCGTGACAAAGGCAACACCATATCCATCCATACCAAGGCTAAGAAAACCAAAACGAGTAGATGTCTATCATTCAAGTTGGCATATAAGACCAGTGAAACCCAGGAATGCAGATCAGCAAATGGGATCAGAAGTGCTTCTATTTCACTACGAAGACATAGGTCTCCCAAGAGAGGTTGTCAAAATGGGCGTCAGGGCAGGAATGTGGAACATGGTAAAGAAACTACATTTAGGAGTTCAGGTTTACAGTATGGAAAAAGCAAATGATGAATCTCCTTCATACCATGCACTTATGGCACACATAACTACAAAGCTTCCGGCGTCACCATCCACAATCATGTATCACATCCCAGAAGTTGAAGCAGATGAGAATGCATGCCAGAGCCAAAACCCACAAGAACAAGTAACAGAGTTCATGGTTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

283

Amino Acids

33.02

Weight (kDa)

6.22

Isoelectric Point (pI)

57.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 37 - 215 5.5e-09 START domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016311)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g22000 FvH4_4g22000 FvH4_4g22000
malus_domestica MD13G1135100.v1.1
prunus_persica Prupe.1G210200_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0428201
rosa_laevigata RLG00000007170
rosa_multiflora Rmu_sc0004940.1_g000004 Rmu_sc0009599.1_g000003
rosa_rugosa Rorug04G0223600
rosa_samantha Rh4AG280800 Rh4BG286300 Rh4CG302000 Rh4DG283300
rosa_wichuraiana Rw4G024390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 326
AccB1I GGYRCC 1 cut(s) 326
AccII CGCG 1 cut(s) 168
AciI CCGC 1 cut(s) 102
AclWI GGATC 2 cut(s) 164, 559
AcsI RAATTY 1 cut(s) 260
AcuI CTGAAG 1 cut(s) 75
AcyI GRCGYC 2 cut(s) 615, 746
AfaI GTAC 3 cut(s) 194, 199, 328
AfiI CCNNNNNNNGG 3 cut(s) 179, 462, 598
AgsI TTSAA 4 cut(s) 67, 407, 501, 785
AjnI CCWGG 2 cut(s) 269, 527
AluBI AGCT 3 cut(s) 158, 355, 739
AluI AGCT 3 cut(s) 158, 355, 739
Alw26I GTCTC 3 cut(s) 82, 86, 593
AlwI GGATC 2 cut(s) 164, 559
AoxI GGCC 1 cut(s) 181
ApeKI GCWGC 1 cut(s) 369
ApoI RAATTY 1 cut(s) 260
ArsI GACNNNNNNTTYG 2 cut(s) 600, 632
Asp700I GAANNNNTTC 1 cut(s) 561
Asp718I GGTACC 1 cut(s) 326
AspS9I GGNCC 2 cut(s) 104, 182
AsuHPI GGTGA 2 cut(s) 216, 741
AsuII TTCGAA 1 cut(s) 213
AvaII GGWCC 1 cut(s) 104
BanI GGYRCC 1 cut(s) 326
BbsI GAAGAC 1 cut(s) 585
BbvI GCAGC 1 cut(s) 381
BccI CCATC 4 cut(s) 92, 118, 460, 760
BciT130I CCWGG 2 cut(s) 271, 529
BcoDI GTCTC 3 cut(s) 82, 86, 593
BfmI CTRYAG 1 cut(s) 367
BisI GCNGC 1 cut(s) 370
BlsI GCNGC 1 cut(s) 371
Bme1390I CCNGG 2 cut(s) 271, 529
Bme18I GGWCC 1 cut(s) 104
BmgT120I GGNCC 2 cut(s) 104, 182
BmiI GGNNCC 1 cut(s) 328
BmrFI CCNGG 2 cut(s) 271, 529
BmsI GCATC 1 cut(s) 422
BoxI GACNNNNGTC 1 cut(s) 585
BpiI GAAGAC 1 cut(s) 585
Bpu14I TTCGAA 1 cut(s) 213
BsaBI GATNNNNATC 1 cut(s) 492
BsaHI GRCGYC 2 cut(s) 615, 746
BsaI GGTCTC 2 cut(s) 82, 593
BsaJI CCNNGG 5 cut(s) 110, 269, 297, 461, 527
BsaXI ACNNNNNCTCC 2 cut(s) 651, 681
Bsc4I CCNNNNNNNGG 3 cut(s) 179, 462, 598
Bse1I ACTGG 2 cut(s) 328, 518
Bse8I GATNNNNATC 1 cut(s) 492
BseBI CCWGG 2 cut(s) 271, 529
BseDI CCNNGG 5 cut(s) 110, 269, 297, 461, 527
BseGI GGATG 4 cut(s) 259, 452, 752, 771
BseJI GATNNNNATC 1 cut(s) 492
BseLI CCNNNNNNNGG 3 cut(s) 179, 462, 598
BseNI ACTGG 2 cut(s) 328, 518
BseRI GAGGAG 1 cut(s) 104
BseXI GCAGC 1 cut(s) 381
Bsh1236I CGCG 1 cut(s) 168
BshFI GGCC 1 cut(s) 183
BshNI GGYRCC 1 cut(s) 326
BsiSI CCGG 1 cut(s) 743
BslI CCNNNNNNNGG 3 cut(s) 179, 462, 598
BsmAI GTCTC 3 cut(s) 82, 86, 593
BsmI GAATGC 2 cut(s) 538, 802
BsnI GGCC 1 cut(s) 183
Bso31I GGTCTC 2 cut(s) 82, 593
Bsp119I TTCGAA 1 cut(s) 213
Bsp143I GATC 3 cut(s) 169, 538, 551
Bsp68I TCGCGA 1 cut(s) 168
BspACI CCGC 1 cut(s) 102
BspANI GGCC 1 cut(s) 183
BspFNI CGCG 1 cut(s) 168
BspHI TCATGA 1 cut(s) 120
BspLI GGNNCC 1 cut(s) 328
BspMAI CTGCAG 1 cut(s) 371
BspPI GGATC 2 cut(s) 164, 559
BspT104I TTCGAA 1 cut(s) 213
BspT107I GGYRCC 1 cut(s) 326
BspTNI GGTCTC 2 cut(s) 82, 593
BsrI ACTGG 2 cut(s) 328, 518
BssECI CCNNGG 5 cut(s) 110, 269, 297, 461, 527
BssMI GATC 3 cut(s) 169, 538, 551
BssNI GRCGYC 2 cut(s) 615, 746
BssT1I CCWWGG 3 cut(s) 110, 297, 461
Bst2UI CCWGG 2 cut(s) 271, 529
Bst4CI ACNGT 5 cut(s) 177, 192, 197, 208, 674
BstACI GRCGYC 2 cut(s) 615, 746
BstBI TTCGAA 1 cut(s) 213
BstC8I GCNNGC 1 cut(s) 802
BstDEI CTNAG 2 cut(s) 305, 467
BstF5I GGATG 4 cut(s) 259, 452, 752, 771
BstFNI CGCG 1 cut(s) 168
BstKTI GATC 3 cut(s) 172, 541, 554
BstMAI GTCTC 3 cut(s) 82, 86, 593
BstMBI GATC 3 cut(s) 169, 538, 551
BstMWI GCNNNNNNNGC 1 cut(s) 719
BstNI CCWGG 2 cut(s) 271, 529
BstNSI RCATGY 1 cut(s) 804
BstPAI GACNNNNGTC 1 cut(s) 585
BstSCI CCNGG 2 cut(s) 269, 527
BstSFI CTRYAG 1 cut(s) 367
BstUI CGCG 1 cut(s) 168
BstV1I GCAGC 1 cut(s) 381
BstV2I GAAGAC 1 cut(s) 585
BsuRI GGCC 1 cut(s) 183
BtsCI GGATG 4 cut(s) 259, 452, 752, 771
BtsIMutI CAGTG 1 cut(s) 525
BtuMI TCGCGA 1 cut(s) 168
Cac8I GCNNGC 1 cut(s) 802
CciI TCATGA 1 cut(s) 120
Cfr13I GGNCC 2 cut(s) 104, 182
CseI GACGC 2 cut(s) 604, 735
Csp6I GTAC 3 cut(s) 193, 198, 327
CviAII CATG 6 cut(s) 121, 637, 710, 763, 801, 841
CviJI RGCY 7 cut(s) 14, 158, 183, 355, 466, 739, 810
CviKI_1 RGCY 7 cut(s) 14, 158, 183, 355, 466, 739, 810
CviQI GTAC 3 cut(s) 193, 198, 327
DdeI CTNAG 2 cut(s) 305, 467
DpnI GATC 3 cut(s) 171, 540, 553
DpnII GATC 3 cut(s) 169, 538, 551
Eco130I CCWWGG 3 cut(s) 110, 297, 461
Eco31I GGTCTC 2 cut(s) 82, 593
Eco47I GGWCC 1 cut(s) 104
Eco57I CTGAAG 1 cut(s) 75
EcoRI GAATTC 1 cut(s) 260
EcoRII CCWGG 2 cut(s) 269, 527
EcoT14I CCWWGG 3 cut(s) 110, 297, 461
EcoT22I ATGCAT 1 cut(s) 802
ErhI CCWWGG 3 cut(s) 110, 297, 461
FaeI CATG 6 cut(s) 124, 640, 713, 766, 804, 844
FatI CATG 6 cut(s) 120, 636, 709, 762, 800, 840
Fnu4HI GCNGC 1 cut(s) 370
FokI GGATG 4 cut(s) 266, 439, 739, 758
Fsp4HI GCNGC 1 cut(s) 370
GluI GCNGC 1 cut(s) 370
HaeIII GGCC 1 cut(s) 183
HapII CCGG 1 cut(s) 743
HgaI GACGC 2 cut(s) 604, 735
Hin1I GRCGYC 2 cut(s) 615, 746
Hin1II CATG 6 cut(s) 124, 640, 713, 766, 804, 844
HindIII AAGCTT 2 cut(s) 156, 737
HinfI GANTC 3 cut(s) 115, 407, 695
HpaII CCGG 1 cut(s) 743
HphI GGTGA 2 cut(s) 216, 741
Hpy166II GTNNAC 2 cut(s) 107, 670
Hpy188I TCNGA 2 cut(s) 266, 556
Hpy188III TCNNGA 3 cut(s) 121, 167, 377
Hpy8I GTNNAC 2 cut(s) 107, 670
Hpy99I CGWCG 1 cut(s) 399
HpyAV CCTTC 2 cut(s) 150, 711
HpyCH4III ACNGT 5 cut(s) 177, 192, 197, 208, 674
HpyCH4V TGCA 5 cut(s) 369, 413, 536, 713, 800
HpyF10VI GCNNNNNNNGC 1 cut(s) 719
HpyF3I CTNAG 2 cut(s) 305, 467
Hsp92I GRCGYC 2 cut(s) 615, 746
Hsp92II CATG 6 cut(s) 124, 640, 713, 766, 804, 844
KpnI GGTACC 1 cut(s) 330
Kzo9I GATC 3 cut(s) 169, 538, 551
LmnI GCTCC 1 cut(s) 127
Lsp1109I GCAGC 1 cut(s) 381
LweI GCATC 1 cut(s) 422
MaeIII GTNAC 4 cut(s) 49, 430, 747, 829
MalI GATC 3 cut(s) 171, 540, 553
MboI GATC 3 cut(s) 169, 538, 551
MboII GAAGA 4 cut(s) 68, 71, 238, 590
MluCI AATT 2 cut(s) 17, 260
MnlI CCTC 4 cut(s) 82, 223, 326, 592
Mph1103I ATGCAT 1 cut(s) 802
MroXI GAANNNNTTC 1 cut(s) 561
MslI CAYNNNNRTG 1 cut(s) 761
MspI CCGG 1 cut(s) 743
MspR9I CCNGG 2 cut(s) 271, 529
Mva1269I GAATGC 2 cut(s) 538, 802
MvaI CCWGG 2 cut(s) 271, 529
MvnI CGCG 1 cut(s) 168
MwoI GCNNNNNNNGC 1 cut(s) 719
NdeII GATC 3 cut(s) 169, 538, 551
NlaIII CATG 6 cut(s) 124, 640, 713, 766, 804, 844
NlaIV GGNNCC 1 cut(s) 328
NmuCI GTSAC 3 cut(s) 49, 430, 747
NruI TCGCGA 1 cut(s) 168
NsiI ATGCAT 1 cut(s) 802
NspI RCATGY 1 cut(s) 804
NspV TTCGAA 1 cut(s) 213
PaeI GCATGC 1 cut(s) 804
PagI TCATGA 1 cut(s) 120
PctI GAATGC 2 cut(s) 538, 802
PdmI GAANNNNTTC 1 cut(s) 561
PfeI GAWTC 3 cut(s) 115, 407, 695
PkrI GCNGC 1 cut(s) 371
PshAI GACNNNNGTC 1 cut(s) 585
Psp6I CCWGG 2 cut(s) 269, 527
PspGI CCWGG 2 cut(s) 269, 527
PspN4I GGNNCC 1 cut(s) 328
PspPI GGNCC 2 cut(s) 104, 182
PstI CTGCAG 1 cut(s) 371
RruI TCGCGA 1 cut(s) 168
RsaI GTAC 3 cut(s) 194, 199, 328
RsaNI GTAC 3 cut(s) 193, 198, 327
RseI CAYNNNNRTG 1 cut(s) 761
SatI GCNGC 1 cut(s) 370
Sau3AI GATC 3 cut(s) 169, 538, 551
Sau96I GGNCC 2 cut(s) 104, 182
ScrFI CCNGG 2 cut(s) 271, 529
SfaNI GCATC 1 cut(s) 422
SfcI CTRYAG 1 cut(s) 367
SfuI TTCGAA 1 cut(s) 213
SinI GGWCC 1 cut(s) 104
SmiMI CAYNNNNRTG 1 cut(s) 761
SphI GCATGC 1 cut(s) 804
Sse9I AATT 2 cut(s) 17, 260
SsiI CCGC 1 cut(s) 102
StyD4I CCNGG 2 cut(s) 269, 527
StyI CCWWGG 3 cut(s) 110, 297, 461
TaaI ACNGT 5 cut(s) 177, 192, 197, 208, 674
TaqI TCGA 2 cut(s) 213, 376
TasI AATT 2 cut(s) 17, 260
TfiI GAWTC 3 cut(s) 115, 407, 695
TscAI CASTG 1 cut(s) 525
TseFI GTSAC 3 cut(s) 49, 430, 747
TseI GCWGC 1 cut(s) 369
Tsp45I GTSAC 3 cut(s) 49, 430, 747
TspDTI ATGAA 6 cut(s) 17, 273, 329, 693, 708, 829
TspGWI ACGGA 2 cut(s) 44, 383
TspRI CASTG 1 cut(s) 525
VpaK11BI GGWCC 1 cut(s) 104
XapI RAATTY 1 cut(s) 260
XceI RCATGY 1 cut(s) 804
XmnI GAANNNNTTC 1 cut(s) 561
Zsp2I ATGCAT 1 cut(s) 802
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.