Prupe.1G210200_v2.0.a1

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0016311
Physical Location & Seq
Forward (+)
22377022 .. 22378851
1830 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G210200.1

Sequence Viewer

Length: 882 bp
ATGATAGCCCAATTGGCATTGGCTCTACTATGTCTTGTTGCAGCATGGATGCCACTTGTGTGCTTGAGTCTTCTAAAGGGATGGTCTTGGTCACGGACCTTATGTTCATGGCGCCTAACATTTCCCACTCTTTCCTTCAGCAGCCACAGGCACAGAGAGATATCATCCGAGTCTGTCAGCCGTCCTCGGCTCACAGCCACCACCTTCAGTGGCCAGTGCTGCACTCACCCACATCACGAACAAGAATGTTCTGAATTGAACGAGGATGATGTGAAGGTATTGGTTTCTCACATCGACGAGACCGATGGCGGTCCACCATGGAAGCTCATGATGGAGCGCTCTACCCCTACTTTGACATACCAAGCTTGGTACAGGGATCCACCGTTGGGCCCTACGCAGTACCGTACAAGAACCGTATTCGAAAATGTATCACCTGAGTTGCTGAGGAACTTCTTCTGGGATGATGAATTTAGACCCCAGTGGGATAACACGCTCATTCACTTCCAAACTCTCAGAGTTTGCCCCAAAACTGGTTCCATGACTGTCCACTGGATTAGAAAGCTGCCATTGTTCTGCAGTGATCGAGAATATGTCATAACCCGTCGCATTTGGGAATCTGATTCTGGATATTACTGCCTAACAAAGGGAACACCATATCCACCCCTACCAAGGTCTAAAAGACCAAGACGAGTAGATCATTATTATTCGAGTTGGCATATAAAATCAGGGGAAAATGAAACGAAAGAATGGCTGAAAAGAAAAGACAAGCCACGGAGAATATCAACGAACGGAGAAATCAAGCAAACAAAGGGGGTTTGGAGAAGATGTCCCCTTCAAGTTCAGCCATTAGACGGCGGCTGCGGGATTGATTGTTATCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

294

Amino Acids

34.39

Weight (kDa)

9.07

Isoelectric Point (pI)

51.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016311)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g22000 FvH4_4g22000 FvH4_4g22000
malus_domestica MD13G1135100.v1.1
prunus_persica Prupe.1G210200_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0428201
rosa_laevigata RLG00000007170
rosa_multiflora Rmu_sc0004940.1_g000004 Rmu_sc0009599.1_g000003
rosa_rugosa Rorug04G0223600
rosa_samantha Rh4AG280800 Rh4BG286300 Rh4CG302000 Rh4DG283300
rosa_wichuraiana Rw4G024390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 111
AciI CCGC 3 cut(s) 309, 855, 861
AclWI GGATC 2 cut(s) 371, 384
AcoI YGGCCR 1 cut(s) 211
AcsI RAATTY 1 cut(s) 467
AcuI CTGAAG 2 cut(s) 121, 190
AcyI GRCGYC 1 cut(s) 112
AfaI GTAC 3 cut(s) 371, 401, 406
AfeI AGCGCT 1 cut(s) 338
AfiI CCNNNNNNNGG 5 cut(s) 386, 530, 643, 669, 851
AgsI TTSAA 2 cut(s) 259, 836
AleI CACNNNNGTG 1 cut(s) 58
AloI GAACNNNNNNTCC 4 cut(s) 88, 120, 640, 672
AluBI AGCT 3 cut(s) 325, 365, 562
AluI AGCT 3 cut(s) 325, 365, 562
Alw26I GTCTC 1 cut(s) 293
AlwI GGATC 2 cut(s) 371, 384
Aor51HI AGCGCT 1 cut(s) 338
AoxI GGCC 2 cut(s) 211, 388
ApaI GGGCCC 1 cut(s) 392
ApeKI GCWGC 5 cut(s) 41, 141, 219, 562, 858
ApoI RAATTY 1 cut(s) 467
Asp700I GAANNNNTTC 1 cut(s) 452
AspLEI GCGC 2 cut(s) 114, 339
AspS9I GGNCC 4 cut(s) 96, 311, 388, 389
AsuHPI GGTGA 2 cut(s) 218, 423
AsuII TTCGAA 1 cut(s) 420
AvaII GGWCC 2 cut(s) 96, 311
BaeGI GKGCMC 1 cut(s) 392
BalI TGGCCA 1 cut(s) 213
BamHI GGATCC 1 cut(s) 376
BanI GGYRCC 1 cut(s) 111
BanII GRGCYC 1 cut(s) 392
BbsI GAAGAC 1 cut(s) 62
BbvCI CCTCAGC 1 cut(s) 443
BbvI GCAGC 5 cut(s) 53, 153, 206, 549, 845
BccI CCATC 3 cut(s) 75, 299, 325
BceAI ACGGC 2 cut(s) 165, 868
BcoDI GTCTC 1 cut(s) 293
BfmI CTRYAG 1 cut(s) 574
BfoI RGCGCY 2 cut(s) 115, 340
BglI GCCNNNNNGGC 1 cut(s) 14
BisI GCNGC 6 cut(s) 42, 142, 220, 563, 856, 859
BlsI GCNGC 6 cut(s) 43, 143, 221, 564, 857, 860
Bme18I GGWCC 2 cut(s) 96, 311
BmgT120I GGNCC 4 cut(s) 96, 311, 388, 389
BmiI GGNNCC 4 cut(s) 113, 378, 390, 535
BmrI ACTGGG 1 cut(s) 472
BmsI GCATC 1 cut(s) 39
BmuI ACTGGG 1 cut(s) 472
BpiI GAAGAC 1 cut(s) 62
Bpu10I CCTNAGC 1 cut(s) 443
Bpu14I TTCGAA 1 cut(s) 420
BpuEI CTTGAG 1 cut(s) 85
Bsa29I ATCGAT 1 cut(s) 877
BsaBI GATNNNNATC 1 cut(s) 873
BsaHI GRCGYC 1 cut(s) 112
BsaI GGTCTC 1 cut(s) 293
BsaJI CCNNGG 4 cut(s) 185, 317, 668, 770
BsaXI ACNNNNNCTCC 2 cut(s) 811, 841
Bsc4I CCNNNNNNNGG 5 cut(s) 386, 530, 643, 669, 851
Bse1I ACTGG 4 cut(s) 214, 478, 535, 554
Bse8I GATNNNNATC 1 cut(s) 873
BseCI ATCGAT 1 cut(s) 877
BseDI CCNNGG 4 cut(s) 185, 317, 668, 770
BseGI GGATG 5 cut(s) 54, 86, 164, 271, 466
BseJI GATNNNNATC 1 cut(s) 873
BseLI CCNNNNNNNGG 5 cut(s) 386, 530, 643, 669, 851
BseMII CTCAG 3 cut(s) 426, 434, 526
BseNI ACTGG 4 cut(s) 214, 478, 535, 554
BseSI GKGCMC 1 cut(s) 392
BseXI GCAGC 5 cut(s) 53, 153, 206, 549, 845
BsgI GTGCAG 1 cut(s) 205
BshFI GGCC 2 cut(s) 213, 390
BshNI GGYRCC 1 cut(s) 111
BshVI ATCGAT 1 cut(s) 877
BslFI GGGAC 1 cut(s) 813
BslI CCNNNNNNNGG 5 cut(s) 386, 530, 643, 669, 851
BsmAI GTCTC 1 cut(s) 293
BsmFI GGGAC 1 cut(s) 813
BsnI GGCC 2 cut(s) 213, 390
Bso31I GGTCTC 1 cut(s) 293
Bsp119I TTCGAA 1 cut(s) 420
Bsp120I GGGCCC 1 cut(s) 388
Bsp1286I GDGCHC 1 cut(s) 392
Bsp143I GATC 3 cut(s) 376, 580, 694
Bsp19I CCATGG 1 cut(s) 317
BspACI CCGC 3 cut(s) 309, 855, 861
BspANI GGCC 2 cut(s) 213, 390
BspCNI CTCAG 3 cut(s) 427, 435, 525
BspDI ATCGAT 1 cut(s) 877
BspHI TCATGA 1 cut(s) 327
BspLI GGNNCC 4 cut(s) 113, 378, 390, 535
BspMAI CTGCAG 1 cut(s) 578
BspPI GGATC 2 cut(s) 371, 384
BspT104I TTCGAA 1 cut(s) 420
BspT107I GGYRCC 1 cut(s) 111
BspTNI GGTCTC 1 cut(s) 293
BsrI ACTGG 4 cut(s) 214, 478, 535, 554
BssECI CCNNGG 4 cut(s) 185, 317, 668, 770
BssMI GATC 3 cut(s) 376, 580, 694
BssNI GRCGYC 1 cut(s) 112
BssT1I CCWWGG 2 cut(s) 317, 668
Bst4CI ACNGT 4 cut(s) 384, 404, 415, 544
BstACI GRCGYC 1 cut(s) 112
BstBI TTCGAA 1 cut(s) 420
BstDEI CTNAG 3 cut(s) 435, 443, 512
BstDSI CCRYGG 2 cut(s) 317, 770
BstENI CCTNNNNNAGG 1 cut(s) 641
BstF5I GGATG 5 cut(s) 54, 86, 164, 271, 466
BstH2I RGCGCY 2 cut(s) 115, 340
BstHHI GCGC 2 cut(s) 114, 339
BstKTI GATC 3 cut(s) 379, 583, 697
BstMAI GTCTC 1 cut(s) 293
BstMBI GATC 3 cut(s) 376, 580, 694
BstMWI GCNNNNNNNGC 2 cut(s) 14, 219
BstSFI CTRYAG 1 cut(s) 574
BstSLI GKGCMC 1 cut(s) 392
BstV1I GCAGC 5 cut(s) 53, 153, 206, 549, 845
BstV2I GAAGAC 1 cut(s) 62
BstX2I RGATCY 1 cut(s) 376
BstYI RGATCY 1 cut(s) 376
Bsu15I ATCGAT 1 cut(s) 877
BsuRI GGCC 2 cut(s) 213, 390
BsuTUI ATCGAT 1 cut(s) 877
BtgI CCRYGG 2 cut(s) 317, 770
BtsCI GGATG 5 cut(s) 54, 86, 164, 271, 466
BtsI GCAGTG 1 cut(s) 583
BtsIMutI CAGTG 5 cut(s) 214, 221, 485, 547, 583
CciI TCATGA 1 cut(s) 327
CfoI GCGC 2 cut(s) 114, 339
Cfr13I GGNCC 4 cut(s) 96, 311, 388, 389
ClaI ATCGAT 1 cut(s) 877
Csp6I GTAC 3 cut(s) 370, 400, 405
CviAII CATG 5 cut(s) 45, 108, 318, 328, 538
CviQI GTAC 3 cut(s) 370, 400, 405
DdeI CTNAG 3 cut(s) 435, 443, 512
DinI GGCGCC 1 cut(s) 113
DpnI GATC 3 cut(s) 378, 582, 696
DpnII GATC 3 cut(s) 376, 580, 694
EaeI YGGCCR 1 cut(s) 211
Eco130I CCWWGG 2 cut(s) 317, 668
Eco24I GRGCYC 1 cut(s) 392
Eco31I GGTCTC 1 cut(s) 293
Eco32I GATATC 1 cut(s) 162
Eco47I GGWCC 2 cut(s) 96, 311
Eco47III AGCGCT 1 cut(s) 338
Eco57I CTGAAG 2 cut(s) 121, 190
EcoNI CCTNNNNNAGG 1 cut(s) 641
EcoO109I RGGNCCY 1 cut(s) 389
EcoRV GATATC 1 cut(s) 162
EcoT14I CCWWGG 2 cut(s) 317, 668
EcoT38I GRGCYC 1 cut(s) 392
EgeI GGCGCC 1 cut(s) 113
EheI GGCGCC 1 cut(s) 113
ErhI CCWWGG 2 cut(s) 317, 668
FaeI CATG 5 cut(s) 48, 111, 321, 331, 541
FaqI GGGAC 1 cut(s) 813
FatI CATG 5 cut(s) 44, 107, 317, 327, 537
FauI CCCGC 1 cut(s) 854
Fnu4HI GCNGC 6 cut(s) 42, 142, 220, 563, 856, 859
FokI GGATG 5 cut(s) 61, 93, 151, 278, 473
FriOI GRGCYC 1 cut(s) 392
Fsp4HI GCNGC 6 cut(s) 42, 142, 220, 563, 856, 859
GlaI GCGC 2 cut(s) 113, 338
GluI GCNGC 6 cut(s) 42, 142, 220, 563, 856, 859
HaeII RGCGCY 2 cut(s) 115, 340
HaeIII GGCC 2 cut(s) 213, 390
HhaI GCGC 2 cut(s) 114, 339
Hin1I GRCGYC 1 cut(s) 112
Hin1II CATG 5 cut(s) 48, 111, 321, 331, 541
Hin6I GCGC 2 cut(s) 112, 337
HinP1I GCGC 2 cut(s) 112, 337
HindIII AAGCTT 1 cut(s) 363
HinfI GANTC 4 cut(s) 67, 170, 614, 620
HphI GGTGA 2 cut(s) 218, 423
Hpy166II GTNNAC 2 cut(s) 314, 547
Hpy188I TCNGA 4 cut(s) 169, 253, 515, 619
Hpy188III TCNNGA 4 cut(s) 236, 328, 584, 624
Hpy8I GTNNAC 2 cut(s) 314, 547
Hpy99I CGWCG 2 cut(s) 299, 606
HpyAV CCTTC 4 cut(s) 145, 214, 268, 842
HpyCH4III ACNGT 4 cut(s) 384, 404, 415, 544
HpyCH4V TGCA 3 cut(s) 41, 222, 576
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 219
HpyF3I CTNAG 3 cut(s) 435, 443, 512
Hsp92I GRCGYC 1 cut(s) 112
Hsp92II CATG 5 cut(s) 48, 111, 321, 331, 541
HspAI GCGC 2 cut(s) 112, 337
KasI GGCGCC 1 cut(s) 111
Kzo9I GATC 3 cut(s) 376, 580, 694
LmnI GCTCC 1 cut(s) 334
Lsp1109I GCAGC 5 cut(s) 53, 153, 206, 549, 845
LweI GCATC 1 cut(s) 39
MaeIII GTNAC 1 cut(s) 90
MalI GATC 3 cut(s) 378, 582, 696
MboI GATC 3 cut(s) 376, 580, 694
MboII GAAGA 3 cut(s) 62, 445, 834
MfeI CAATTG 1 cut(s) 11
MflI RGATCY 1 cut(s) 376
MhlI GDGCHC 1 cut(s) 392
MlsI TGGCCA 1 cut(s) 213
MluCI AATT 3 cut(s) 11, 254, 467
MluNI TGGCCA 1 cut(s) 213
Mly113I GGCGCC 1 cut(s) 112
MlyI GAGTC 2 cut(s) 76, 179
MnlI CCTC 3 cut(s) 195, 256, 438
Mox20I TGGCCA 1 cut(s) 213
MroXI GAANNNNTTC 1 cut(s) 452
MscI TGGCCA 1 cut(s) 213
MslI CAYNNNNRTG 1 cut(s) 58
Msp20I TGGCCA 1 cut(s) 213
MunI CAATTG 1 cut(s) 11
MwoI GCNNNNNNNGC 2 cut(s) 14, 219
NarI GGCGCC 1 cut(s) 112
NcoI CCATGG 1 cut(s) 317
NdeII GATC 3 cut(s) 376, 580, 694
NlaIII CATG 5 cut(s) 48, 111, 321, 331, 541
NlaIV GGNNCC 4 cut(s) 113, 378, 390, 535
NmeAIII GCCGAG 1 cut(s) 166
NmuCI GTSAC 1 cut(s) 90
NspV TTCGAA 1 cut(s) 420
OliI CACNNNNGTG 1 cut(s) 58
PagI TCATGA 1 cut(s) 327
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PdmI GAANNNNTTC 1 cut(s) 452
PfeI GAWTC 2 cut(s) 614, 620
PkrI GCNGC 6 cut(s) 43, 143, 221, 564, 857, 860
PleI GAGTC 2 cut(s) 75, 178
PluTI GGCGCC 1 cut(s) 115
PpsI GAGTC 2 cut(s) 75, 178
PspN4I GGNNCC 4 cut(s) 113, 378, 390, 535
PspOMI GGGCCC 1 cut(s) 388
PspPI GGNCC 4 cut(s) 96, 311, 388, 389
PstI CTGCAG 1 cut(s) 578
PsuI RGATCY 1 cut(s) 376
RsaI GTAC 3 cut(s) 371, 401, 406
RsaNI GTAC 3 cut(s) 370, 400, 405
RseI CAYNNNNRTG 1 cut(s) 58
SatI GCNGC 6 cut(s) 42, 142, 220, 563, 856, 859
Sau3AI GATC 3 cut(s) 376, 580, 694
Sau96I GGNCC 4 cut(s) 96, 311, 388, 389
SchI GAGTC 2 cut(s) 76, 179
SduI GDGCHC 1 cut(s) 392
SetI ASST 8 cut(s) 101, 206, 279, 327, 367, 436, 564, 674
SfaNI GCATC 1 cut(s) 39
SfcI CTRYAG 1 cut(s) 574
SfoI GGCGCC 1 cut(s) 113
SfuI TTCGAA 1 cut(s) 420
SinI GGWCC 2 cut(s) 96, 311
SmiMI CAYNNNNRTG 1 cut(s) 58
SmlI CTYRAG 1 cut(s) 64
SmoI CTYRAG 1 cut(s) 64
Sse9I AATT 3 cut(s) 11, 254, 467
SsiI CCGC 3 cut(s) 309, 855, 861
SspDI GGCGCC 1 cut(s) 111
StyI CCWWGG 2 cut(s) 317, 668
TaaI ACNGT 4 cut(s) 384, 404, 415, 544
TaqI TCGA 5 cut(s) 294, 420, 583, 707, 877
TaqII GACCGA 1 cut(s) 317
TasI AATT 3 cut(s) 11, 254, 467
TauI GCSGC 1 cut(s) 858
TfiI GAWTC 2 cut(s) 614, 620
TscAI CASTG 5 cut(s) 214, 221, 485, 554, 583
TseFI GTSAC 1 cut(s) 90
TseI GCWGC 5 cut(s) 41, 141, 219, 562, 858
Tsp45I GTSAC 1 cut(s) 90
TspDTI ATGAA 3 cut(s) 96, 480, 750
TspGWI ACGGA 3 cut(s) 109, 787, 804
TspRI CASTG 5 cut(s) 214, 221, 485, 554, 583
VpaK11BI GGWCC 2 cut(s) 96, 311
XagI CCTNNNNNAGG 1 cut(s) 641
XapI RAATTY 1 cut(s) 467
XmnI GAANNNNTTC 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.