Rh4CG302000

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0016311
Physical Location & Seq
Forward (+)
56244838 .. 56246428
1591 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG302000.1

Sequence Viewer

Length: 1182 bp
ATGAATATGATAGCCGAATTACTGTTGACGGTAGTCTGTTTTGTAGCAGCAGCACTGATACCACTTGTGAGCTTAAGCCTTATCAAGGGATGTTCTTGGACATGGTCGTGGACCTCATGCTCATGGTCATGGTCGAGTTCATGGCGCCTACCATTTGTGACTCTCTCCCTCCATGATTCTGCTCTGGGAAGACTGCTCTCTTGCTTCAACAGCCACAGACAGAGAGTACTATCCGAGTTAGTCGACAACTCTTATCCTCCTGCTGATCAGGAACAAGAACCTTGTGAAGTGAATGAAGAAGATTTGAAAGTACTGGTCTCTCATATTGAGGAGACTGATGGCGGTCCACCTTGGAATCTCATGATGGAGCAGTCTATTCCTTCCTTGACATACAAAGCTTGGTATCGTGATCCACCGTTAGGCCCGACACAGTACCGTACTAAAACAGTTTTCGAAAATGTATCACCAGAGGTATTGAGGGATTTCTTCTGGGATGATGAATTCAGACCCAGGTGGGATAAGACGCTCATTCACACCAAGGTTCTAAGCGTTTGCCTCCAAACTGGTACCATGATTGTTCATTGGATTAGAAAGCTACCACTACTCTGCTGCAATCGAGAATATATCATAATTCGTCGCATTTTTGAATCTGCATCAGGGTATTATTGCGTGACAAAGGCAACACCATATCCATCCATACCAAGGCTAAGAAAACCAAAACGAGTAGATGTCTATCATTCAAGTTGGCATATAAGGCCAGGTATGTATTTAACTTCATTTTTGTCACGGTTTCTGTTCACTAGTAGAAGTCTATTGCGCTCAAGCATTACATCGAAAACCTATCTTTCAGTGAAACCCAGGAATGCAGATCAGCAAATGGGATCAGAAGTGCTTCTATTTCACTACGAAGACATACGTCTCCCAAGAGAGGTTGTCAAAATGGGCGTCAGGGCAGGAATGTGGAACATGGTAAAGAAACTACATTTAGGAGTTCAGGTTTACAGTATGGAAAAAGCAAATGATGAATCTCTTTCATACCATGCAATTATGGCACACATAACTACAAAGCTTCCGGCTTCACCATCCACAATCATGTATCACATCCCAGAAGTTGAAGCAGATGAGAATGCATGCCAGAGCCAAAACCCACAAGAACAAGTAACAGAGTTCATAGTTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

393

Amino Acids

45.43

Weight (kDa)

7.6

Isoelectric Point (pI)

49.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 118 - 246 7.2e-07 START domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016311)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g22000 FvH4_4g22000 FvH4_4g22000
malus_domestica MD13G1135100.v1.1
prunus_persica Prupe.1G210200_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0428201
rosa_laevigata RLG00000007170
rosa_multiflora Rmu_sc0004940.1_g000004 Rmu_sc0009599.1_g000003
rosa_rugosa Rorug04G0223600
rosa_samantha Rh4AG280800 Rh4BG286300 Rh4CG302000 Rh4DG283300
rosa_wichuraiana Rw4G024390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 566
AccB1I GGYRCC 2 cut(s) 144, 566
AccI GTMKAC 1 cut(s) 243
AciI CCGC 1 cut(s) 342
AclWI GGATC 2 cut(s) 404, 889
AcsI RAATTY 1 cut(s) 500
AcyI GRCGYC 2 cut(s) 145, 945
AfaI GTAC 5 cut(s) 228, 312, 434, 439, 568
AfiI CCNNNNNNNGG 4 cut(s) 85, 419, 702, 928
AflII CTTAAG 1 cut(s) 73
AgsI TTSAA 5 cut(s) 208, 307, 647, 741, 1115
AhlI ACTAGT 1 cut(s) 800
AjnI CCWGG 3 cut(s) 509, 757, 857
AluBI AGCT 4 cut(s) 72, 398, 595, 1069
AluI AGCT 4 cut(s) 72, 398, 595, 1069
Alw26I GTCTC 3 cut(s) 322, 326, 923
AlwI GGATC 2 cut(s) 404, 889
AoxI GGCC 2 cut(s) 421, 755
ApeKI GCWGC 3 cut(s) 47, 50, 609
ApoI RAATTY 1 cut(s) 500
ArsI GACNNNNNNTTYG 2 cut(s) 930, 962
Asp700I GAANNNNTTC 1 cut(s) 891
Asp718I GGTACC 1 cut(s) 566
AspLEI GCGC 2 cut(s) 147, 819
AspS9I GGNCC 3 cut(s) 111, 344, 422
AsuHPI GGTGA 2 cut(s) 456, 1071
AsuII TTCGAA 1 cut(s) 453
AvaII GGWCC 2 cut(s) 111, 344
BanI GGYRCC 2 cut(s) 144, 566
BbsI GAAGAC 2 cut(s) 196, 915
BbvI GCAGC 3 cut(s) 59, 62, 596
BccI CCATC 4 cut(s) 332, 358, 700, 1090
BciT130I CCWGG 3 cut(s) 511, 759, 859
BclI TGATCA 1 cut(s) 265
BcoDI GTCTC 3 cut(s) 322, 326, 923
BcuI ACTAGT 1 cut(s) 800
BfaI CTAG 1 cut(s) 801
BfoI RGCGCY 1 cut(s) 148
BfrI CTTAAG 1 cut(s) 73
BisI GCNGC 3 cut(s) 48, 51, 610
BlsI GCNGC 3 cut(s) 49, 52, 611
BmcAI AGTACT 2 cut(s) 228, 312
Bme1390I CCNGG 3 cut(s) 511, 759, 859
Bme18I GGWCC 2 cut(s) 111, 344
BmgT120I GGNCC 3 cut(s) 111, 344, 422
BmiI GGNNCC 2 cut(s) 146, 568
BmrFI CCNGG 3 cut(s) 511, 759, 859
BmsI GCATC 1 cut(s) 662
BoxI GACNNNNGTC 2 cut(s) 32, 915
BpiI GAAGAC 2 cut(s) 196, 915
Bpu14I TTCGAA 1 cut(s) 453
BpuEI CTTGAG 1 cut(s) 805
BsaBI GATNNNNATC 1 cut(s) 732
BsaHI GRCGYC 2 cut(s) 145, 945
BsaI GGTCTC 1 cut(s) 322
BsaJI CCNNGG 5 cut(s) 350, 509, 537, 701, 857
BsaXI ACNNNNNCTCC 2 cut(s) 981, 1011
Bsc4I CCNNNNNNNGG 4 cut(s) 85, 419, 702, 928
Bse1I ACTGG 2 cut(s) 318, 568
Bse8I GATNNNNATC 1 cut(s) 732
BseBI CCWGG 3 cut(s) 511, 759, 859
BseDI CCNNGG 5 cut(s) 350, 509, 537, 701, 857
BseGI GGATG 5 cut(s) 95, 499, 692, 1082, 1101
BseJI GATNNNNATC 1 cut(s) 732
BseLI CCNNNNNNNGG 4 cut(s) 85, 419, 702, 928
BseNI ACTGG 2 cut(s) 318, 568
BseRI GAGGAG 1 cut(s) 344
BseXI GCAGC 3 cut(s) 59, 62, 596
BshFI GGCC 2 cut(s) 423, 757
BshNI GGYRCC 2 cut(s) 144, 566
BsiSI CCGG 1 cut(s) 1073
BslI CCNNNNNNNGG 4 cut(s) 85, 419, 702, 928
BsmAI GTCTC 3 cut(s) 322, 326, 923
BsmBI CGTCTC 1 cut(s) 923
BsmI GAATGC 2 cut(s) 868, 1132
BsnI GGCC 2 cut(s) 423, 757
Bso31I GGTCTC 1 cut(s) 322
Bsp119I TTCGAA 1 cut(s) 453
Bsp143I GATC 4 cut(s) 265, 409, 868, 881
BspACI CCGC 1 cut(s) 342
BspANI GGCC 2 cut(s) 423, 757
BspHI TCATGA 1 cut(s) 360
BspLI GGNNCC 2 cut(s) 146, 568
BspPI GGATC 2 cut(s) 404, 889
BspT104I TTCGAA 1 cut(s) 453
BspT107I GGYRCC 2 cut(s) 144, 566
BspTI CTTAAG 1 cut(s) 73
BspTNI GGTCTC 1 cut(s) 322
BsrI ACTGG 2 cut(s) 318, 568
BssECI CCNNGG 5 cut(s) 350, 509, 537, 701, 857
BssMI GATC 4 cut(s) 265, 409, 868, 881
BssNI GRCGYC 2 cut(s) 145, 945
BssT1I CCWWGG 3 cut(s) 350, 537, 701
Bst2UI CCWGG 3 cut(s) 511, 759, 859
Bst4CI ACNGT 8 cut(s) 24, 31, 417, 432, 437, 448, 789, 1004
BstACI GRCGYC 2 cut(s) 145, 945
BstAFI CTTAAG 1 cut(s) 73
BstBI TTCGAA 1 cut(s) 453
BstC8I GCNNGC 1 cut(s) 1132
BstDEI CTNAG 2 cut(s) 545, 707
BstENI CCTNNNNNAGG 1 cut(s) 83
BstF5I GGATG 5 cut(s) 95, 499, 692, 1082, 1101
BstH2I RGCGCY 1 cut(s) 148
BstHHI GCGC 2 cut(s) 147, 819
BstKTI GATC 4 cut(s) 268, 412, 871, 884
BstMAI GTCTC 3 cut(s) 322, 326, 923
BstMBI GATC 4 cut(s) 265, 409, 868, 881
BstMWI GCNNNNNNNGC 3 cut(s) 210, 754, 1049
BstNI CCWGG 3 cut(s) 511, 759, 859
BstNSI RCATGY 1 cut(s) 1134
BstPAI GACNNNNGTC 2 cut(s) 32, 915
BstSCI CCNGG 3 cut(s) 509, 757, 857
BstV1I GCAGC 3 cut(s) 59, 62, 596
BstV2I GAAGAC 2 cut(s) 196, 915
BsuRI GGCC 2 cut(s) 423, 757
BtsCI GGATG 5 cut(s) 95, 499, 692, 1082, 1101
BtsIMutI CAGTG 2 cut(s) 53, 855
Cac8I GCNNGC 1 cut(s) 1132
CciI TCATGA 1 cut(s) 360
CfoI GCGC 2 cut(s) 147, 819
Cfr13I GGNCC 3 cut(s) 111, 344, 422
CseI GACGC 2 cut(s) 532, 934
Csp6I GTAC 5 cut(s) 227, 311, 433, 438, 567
CviQI GTAC 5 cut(s) 227, 311, 433, 438, 567
DdeI CTNAG 2 cut(s) 545, 707
DinI GGCGCC 1 cut(s) 146
DpnI GATC 4 cut(s) 267, 411, 870, 883
DpnII GATC 4 cut(s) 265, 409, 868, 881
Eco130I CCWWGG 3 cut(s) 350, 537, 701
Eco31I GGTCTC 1 cut(s) 322
Eco47I GGWCC 2 cut(s) 111, 344
EcoNI CCTNNNNNAGG 1 cut(s) 83
EcoRI GAATTC 1 cut(s) 500
EcoRII CCWGG 3 cut(s) 509, 757, 857
EcoT14I CCWWGG 3 cut(s) 350, 537, 701
EcoT22I ATGCAT 1 cut(s) 1132
EgeI GGCGCC 1 cut(s) 146
EheI GGCGCC 1 cut(s) 146
ErhI CCWWGG 3 cut(s) 350, 537, 701
Esp3I CGTCTC 1 cut(s) 923
FbaI TGATCA 1 cut(s) 265
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 3 cut(s) 48, 51, 610
FokI GGATG 5 cut(s) 102, 506, 679, 1069, 1088
Fsp4HI GCNGC 3 cut(s) 48, 51, 610
FspBI CTAG 1 cut(s) 801
GlaI GCGC 2 cut(s) 146, 818
GluI GCNGC 3 cut(s) 48, 51, 610
HaeII RGCGCY 1 cut(s) 148
HaeIII GGCC 2 cut(s) 423, 757
HapII CCGG 1 cut(s) 1073
HgaI GACGC 2 cut(s) 532, 934
HhaI GCGC 2 cut(s) 147, 819
Hin1I GRCGYC 2 cut(s) 145, 945
Hin6I GCGC 2 cut(s) 145, 817
HinP1I GCGC 2 cut(s) 145, 817
HincII GTYRAC 2 cut(s) 27, 244
HindII GTYRAC 2 cut(s) 27, 244
HindIII AAGCTT 2 cut(s) 396, 1067
HinfI GANTC 5 cut(s) 160, 176, 355, 647, 1025
HpaII CCGG 1 cut(s) 1073
HphI GGTGA 2 cut(s) 456, 1071
Hpy166II GTNNAC 6 cut(s) 27, 111, 244, 347, 798, 1000
Hpy188I TCNGA 3 cut(s) 235, 506, 886
Hpy188III TCNNGA 4 cut(s) 269, 361, 407, 617
Hpy8I GTNNAC 6 cut(s) 27, 111, 244, 347, 798, 1000
Hpy99I CGWCG 1 cut(s) 639
HpyAV CCTTC 1 cut(s) 390
HpyCH4III ACNGT 8 cut(s) 24, 31, 417, 432, 437, 448, 789, 1004
HpyCH4IV ACGT 1 cut(s) 916
HpyCH4V TGCA 5 cut(s) 612, 653, 866, 1043, 1130
HpyF10VI GCNNNNNNNGC 3 cut(s) 210, 754, 1049
HpyF3I CTNAG 2 cut(s) 545, 707
HpySE526I ACGT 1 cut(s) 916
Hsp92I GRCGYC 2 cut(s) 145, 945
HspAI GCGC 2 cut(s) 145, 817
KasI GGCGCC 1 cut(s) 144
KpnI GGTACC 1 cut(s) 570
Ksp22I TGATCA 1 cut(s) 265
Kzo9I GATC 4 cut(s) 265, 409, 868, 881
LmnI GCTCC 1 cut(s) 367
Lsp1109I GCAGC 3 cut(s) 59, 62, 596
LweI GCATC 1 cut(s) 662
MaeI CTAG 1 cut(s) 801
MaeII ACGT 1 cut(s) 916
MaeIII GTNAC 4 cut(s) 157, 670, 783, 1159
MalI GATC 4 cut(s) 267, 411, 870, 883
MboI GATC 4 cut(s) 265, 409, 868, 881
MboII GAAGA 5 cut(s) 201, 308, 311, 478, 920
MluCI AATT 4 cut(s) 17, 500, 630, 1044
Mly113I GGCGCC 1 cut(s) 145
MlyI GAGTC 1 cut(s) 154
MnlI CCTC 8 cut(s) 124, 179, 267, 322, 463, 471, 566, 922
Mph1103I ATGCAT 1 cut(s) 1132
MroXI GAANNNNTTC 1 cut(s) 891
MseI TTAA 2 cut(s) 74, 770
MslI CAYNNNNRTG 4 cut(s) 106, 121, 127, 1091
MspCI CTTAAG 1 cut(s) 73
MspI CCGG 1 cut(s) 1073
MspR9I CCNGG 3 cut(s) 511, 759, 859
Mva1269I GAATGC 2 cut(s) 868, 1132
MvaI CCWGG 3 cut(s) 511, 759, 859
MwoI GCNNNNNNNGC 3 cut(s) 210, 754, 1049
NarI GGCGCC 1 cut(s) 145
NdeII GATC 4 cut(s) 265, 409, 868, 881
NlaIV GGNNCC 2 cut(s) 146, 568
NmuCI GTSAC 3 cut(s) 157, 670, 783
NsiI ATGCAT 1 cut(s) 1132
NspI RCATGY 1 cut(s) 1134
NspV TTCGAA 1 cut(s) 453
PaeI GCATGC 1 cut(s) 1134
PagI TCATGA 1 cut(s) 360
PctI GAATGC 2 cut(s) 868, 1132
PdmI GAANNNNTTC 1 cut(s) 891
PfeI GAWTC 4 cut(s) 176, 355, 647, 1025
PflFI GACNNNGTC 1 cut(s) 103
PkrI GCNGC 3 cut(s) 49, 52, 611
PleI GAGTC 1 cut(s) 154
PluTI GGCGCC 1 cut(s) 148
PpsI GAGTC 1 cut(s) 154
PshAI GACNNNNGTC 2 cut(s) 32, 915
Psp6I CCWGG 3 cut(s) 509, 757, 857
PspGI CCWGG 3 cut(s) 509, 757, 857
PspN4I GGNNCC 2 cut(s) 146, 568
PspPI GGNCC 3 cut(s) 111, 344, 422
PsyI GACNNNGTC 1 cut(s) 103
RsaI GTAC 5 cut(s) 228, 312, 434, 439, 568
RsaNI GTAC 5 cut(s) 227, 311, 433, 438, 567
RseI CAYNNNNRTG 4 cut(s) 106, 121, 127, 1091
SalI GTCGAC 1 cut(s) 242
SaqAI TTAA 2 cut(s) 74, 770
SatI GCNGC 3 cut(s) 48, 51, 610
Sau3AI GATC 4 cut(s) 265, 409, 868, 881
Sau96I GGNCC 3 cut(s) 111, 344, 422
ScaI AGTACT 2 cut(s) 228, 312
SchI GAGTC 1 cut(s) 154
ScrFI CCNGG 3 cut(s) 511, 759, 859
SfaNI GCATC 1 cut(s) 662
SfoI GGCGCC 1 cut(s) 146
SfuI TTCGAA 1 cut(s) 453
SinI GGWCC 2 cut(s) 111, 344
SmiMI CAYNNNNRTG 4 cut(s) 106, 121, 127, 1091
SmlI CTYRAG 2 cut(s) 73, 820
SmoI CTYRAG 2 cut(s) 73, 820
SpeI ACTAGT 1 cut(s) 800
SphI GCATGC 1 cut(s) 1134
Sse9I AATT 4 cut(s) 17, 500, 630, 1044
SsiI CCGC 1 cut(s) 342
SspDI GGCGCC 1 cut(s) 144
SspMI CTAG 1 cut(s) 801
StyD4I CCNGG 3 cut(s) 509, 757, 857
StyI CCWWGG 3 cut(s) 350, 537, 701
TaaI ACNGT 8 cut(s) 24, 31, 417, 432, 437, 448, 789, 1004
TaiI ACGT 1 cut(s) 919
TaqI TCGA 5 cut(s) 134, 243, 453, 616, 833
TasI AATT 4 cut(s) 17, 500, 630, 1044
TatI WGTACW 2 cut(s) 226, 310
TfiI GAWTC 4 cut(s) 176, 355, 647, 1025
Tru1I TTAA 2 cut(s) 74, 770
Tru9I TTAA 2 cut(s) 74, 770
TscAI CASTG 2 cut(s) 60, 855
TseFI GTSAC 3 cut(s) 157, 670, 783
TseI GCWGC 3 cut(s) 47, 50, 609
Tsp45I GTSAC 3 cut(s) 157, 670, 783
TspDTI ATGAA 9 cut(s) 17, 129, 309, 513, 569, 765, 1023, 1038, 1159
TspRI CASTG 2 cut(s) 60, 855
Tth111I GACNNNGTC 1 cut(s) 103
Vha464I CTTAAG 1 cut(s) 73
VpaK11BI GGWCC 2 cut(s) 111, 344
XagI CCTNNNNNAGG 1 cut(s) 83
XapI RAATTY 1 cut(s) 500
XceI RCATGY 1 cut(s) 1134
XmiI GTMKAC 1 cut(s) 243
XmnI GAANNNNTTC 1 cut(s) 891
XspI CTAG 1 cut(s) 801
ZrmI AGTACT 2 cut(s) 228, 312
Zsp2I ATGCAT 1 cut(s) 1132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.