MD13G1135100.v1.1

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0016311
Physical Location & Seq
Reverse (-)
10417482 .. 10418223
742 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1135100.v1.1.491

Sequence Viewer

Length: 462 bp
ATGATATCCCAATTGGCGCTGGCAGCAGTCTCTGTTGTTTTTGGAGCACTGGTGCCACTTTTTATGGCCTTAATTGTTATCAAGGGATGGTCTTGGTCTTGGCATTGGTCATGGTCCTGGCGGATTTCGTGGCAACCAAGAATTGTGTATTGCTTACGCGGCCACGAATATGGAGCTACATCCTCCGAGTATGCAAGCCACCGTCCTAGGCTCACAGCCACCTCCGGTGGCCAGTCTTGTAGTCAGCCTCTTGATCATGAACAAGAATGCTGCAAATTGGATGAGGATGATCTGAAAGTATTGGTTTCCCACATAAACGAGACCAACGGATGTCCACCGTGGAAGCTCATGATGGAGCATTCTACCCCTTCTCTGACATACCAAGCTTGGTACAGGGACCCGCGGGTCCTACGCAGTACCGTACGAGAACAGTGTTTGAAAACGCATCGCCTGAGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

154

Amino Acids

17.62

Weight (kDa)

8.2

Isoelectric Point (pI)

41.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016311)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g22000 FvH4_4g22000 FvH4_4g22000
malus_domestica MD13G1135100.v1.1
prunus_persica Prupe.1G210200_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0428201
rosa_laevigata RLG00000007170
rosa_multiflora Rmu_sc0004940.1_g000004 Rmu_sc0009599.1_g000003
rosa_rugosa Rorug04G0223600
rosa_samantha Rh4AG280800 Rh4BG286300 Rh4CG302000 Rh4DG283300
rosa_wichuraiana Rw4G024390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 404
AccB1I GGYRCC 1 cut(s) 52
AccII CGCG 2 cut(s) 159, 403
AciI CCGC 4 cut(s) 121, 159, 401, 403
AcoI YGGCCR 2 cut(s) 160, 229
AfaI GTAC 3 cut(s) 392, 418, 423
AgsI TTSAA 1 cut(s) 439
AjnI CCWGG 1 cut(s) 116
AluBI AGCT 3 cut(s) 176, 346, 386
AluI AGCT 3 cut(s) 176, 346, 386
Alw21I GWGCWC 1 cut(s) 49
Alw26I GTCTC 2 cut(s) 34, 314
AlwNI CAGNNNCTG 1 cut(s) 32
AoxI GGCC 3 cut(s) 66, 160, 229
ApeKI GCWGC 2 cut(s) 23, 270
AspA2I CCTAGG 1 cut(s) 206
AspLEI GCGC 1 cut(s) 19
AspS9I GGNCC 3 cut(s) 114, 397, 406
AvaII GGWCC 3 cut(s) 114, 397, 406
AvrII CCTAGG 1 cut(s) 206
BalI TGGCCA 1 cut(s) 231
BanI GGYRCC 1 cut(s) 52
Bbv12I GWGCWC 1 cut(s) 49
BbvI GCAGC 2 cut(s) 35, 257
BccI CCATC 2 cut(s) 81, 346
BciT130I CCWGG 1 cut(s) 118
BclI TGATCA 1 cut(s) 253
BcoDI GTCTC 2 cut(s) 34, 314
BfaI CTAG 1 cut(s) 207
BfoI RGCGCY 1 cut(s) 20
BisI GCNGC 3 cut(s) 24, 160, 271
BlnI CCTAGG 1 cut(s) 206
BlsI GCNGC 3 cut(s) 25, 161, 272
Bme1390I CCNGG 1 cut(s) 118
Bme18I GGWCC 3 cut(s) 114, 397, 406
BmgT120I GGNCC 3 cut(s) 114, 397, 406
BmiI GGNNCC 4 cut(s) 54, 398, 399, 407
BmrFI CCNGG 1 cut(s) 118
BmsI GCATC 1 cut(s) 454
BsaI GGTCTC 1 cut(s) 314
BsaJI CCNNGG 3 cut(s) 206, 338, 401
BsaWI WCCGGW 1 cut(s) 224
BsaXI ACNNNNNCTCC 2 cut(s) 36, 66
Bse1I ACTGG 2 cut(s) 54, 232
BseBI CCWGG 1 cut(s) 118
BseDI CCNNGG 3 cut(s) 206, 338, 401
BseGI GGATG 5 cut(s) 92, 179, 286, 292, 335
BseMII CTCAG 1 cut(s) 443
BseNI ACTGG 2 cut(s) 54, 232
BseXI GCAGC 2 cut(s) 35, 257
Bsh1236I CGCG 2 cut(s) 159, 403
BshFI GGCC 3 cut(s) 68, 162, 231
BshNI GGYRCC 1 cut(s) 52
BsiHKAI GWGCWC 1 cut(s) 49
BsiSI CCGG 1 cut(s) 225
BsiWI CGTACG 1 cut(s) 421
BslFI GGGAC 1 cut(s) 410
BsmAI GTCTC 2 cut(s) 34, 314
BsmFI GGGAC 1 cut(s) 410
BsmI GAATGC 2 cut(s) 272, 358
BsnI GGCC 3 cut(s) 68, 162, 231
Bso31I GGTCTC 1 cut(s) 314
Bsp1286I GDGCHC 1 cut(s) 49
Bsp143I GATC 2 cut(s) 253, 289
BspACI CCGC 4 cut(s) 121, 159, 401, 403
BspANI GGCC 3 cut(s) 68, 162, 231
BspCNI CTCAG 1 cut(s) 444
BspFNI CGCG 2 cut(s) 159, 403
BspHI TCATGA 2 cut(s) 256, 348
BspLI GGNNCC 4 cut(s) 54, 398, 399, 407
BspT107I GGYRCC 1 cut(s) 52
BspTNI GGTCTC 1 cut(s) 314
BsrI ACTGG 2 cut(s) 54, 232
BssECI CCNNGG 3 cut(s) 206, 338, 401
BssMI GATC 2 cut(s) 253, 289
BssT1I CCWWGG 1 cut(s) 206
Bst2UI CCWGG 1 cut(s) 118
Bst4CI ACNGT 4 cut(s) 203, 339, 421, 432
BstC8I GCNNGC 2 cut(s) 21, 196
BstDEI CTNAG 1 cut(s) 452
BstDSI CCRYGG 2 cut(s) 338, 401
BstF5I GGATG 5 cut(s) 92, 179, 286, 292, 335
BstFNI CGCG 2 cut(s) 159, 403
BstH2I RGCGCY 1 cut(s) 20
BstHHI GCGC 1 cut(s) 19
BstKTI GATC 2 cut(s) 256, 292
BstMAI GTCTC 2 cut(s) 34, 314
BstMBI GATC 2 cut(s) 253, 289
BstMWI GCNNNNNNNGC 3 cut(s) 23, 159, 456
BstNI CCWGG 1 cut(s) 118
BstSCI CCNGG 1 cut(s) 116
BstUI CGCG 2 cut(s) 159, 403
BstV1I GCAGC 2 cut(s) 35, 257
BstXI CCANNNNNNTGG 1 cut(s) 170
BsuRI GGCC 3 cut(s) 68, 162, 231
BtgI CCRYGG 2 cut(s) 338, 401
BtgZI GCGATG 1 cut(s) 431
BtsCI GGATG 5 cut(s) 92, 179, 286, 292, 335
BtsIMutI CAGTG 2 cut(s) 47, 437
Cac8I GCNNGC 2 cut(s) 21, 196
CaiI CAGNNNCTG 1 cut(s) 32
CciI TCATGA 2 cut(s) 256, 348
CfoI GCGC 1 cut(s) 19
Cfr13I GGNCC 3 cut(s) 114, 397, 406
Cfr42I CCGCGG 1 cut(s) 404
Csp6I GTAC 3 cut(s) 391, 417, 422
CviAII CATG 3 cut(s) 111, 257, 349
CviQI GTAC 3 cut(s) 391, 417, 422
DdeI CTNAG 1 cut(s) 452
DpnI GATC 2 cut(s) 255, 291
DpnII GATC 2 cut(s) 253, 289
DrdI GACNNNNNNGTC 1 cut(s) 404
DseDI GACNNNNNNGTC 1 cut(s) 404
EaeI YGGCCR 2 cut(s) 160, 229
EciI GGCGGA 1 cut(s) 136
Eco130I CCWWGG 1 cut(s) 206
Eco31I GGTCTC 1 cut(s) 314
Eco32I GATATC 1 cut(s) 6
Eco47I GGWCC 3 cut(s) 114, 397, 406
EcoO109I RGGNCCY 2 cut(s) 397, 406
EcoRII CCWGG 1 cut(s) 116
EcoRV GATATC 1 cut(s) 6
EcoT14I CCWWGG 1 cut(s) 206
ErhI CCWWGG 1 cut(s) 206
FaeI CATG 3 cut(s) 114, 260, 352
FaiI YATR 8 cut(s) 65, 112, 171, 192, 258, 314, 350, 379
FaqI GGGAC 1 cut(s) 410
FatI CATG 3 cut(s) 110, 256, 348
FauI CCCGC 2 cut(s) 396, 408
FbaI TGATCA 1 cut(s) 253
Fnu4HI GCNGC 3 cut(s) 24, 160, 271
FokI GGATG 5 cut(s) 99, 166, 293, 299, 342
Fsp4HI GCNGC 3 cut(s) 24, 160, 271
FspBI CTAG 1 cut(s) 207
GlaI GCGC 1 cut(s) 18
GluI GCNGC 3 cut(s) 24, 160, 271
HaeII RGCGCY 1 cut(s) 20
HaeIII GGCC 3 cut(s) 68, 162, 231
HapII CCGG 1 cut(s) 225
HhaI GCGC 1 cut(s) 19
Hin1II CATG 3 cut(s) 114, 260, 352
Hin6I GCGC 1 cut(s) 17
HinP1I GCGC 1 cut(s) 17
HindIII AAGCTT 1 cut(s) 384
HpaII CCGG 1 cut(s) 225
Hpy166II GTNNAC 1 cut(s) 335
Hpy188I TCNGA 3 cut(s) 187, 294, 375
Hpy188III TCNNGA 3 cut(s) 251, 257, 349
Hpy8I GTNNAC 1 cut(s) 335
HpyAV CCTTC 1 cut(s) 378
HpyCH4III ACNGT 4 cut(s) 203, 339, 421, 432
HpyCH4V TGCA 2 cut(s) 194, 273
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 159, 456
HpyF3I CTNAG 1 cut(s) 452
Hsp92II CATG 3 cut(s) 114, 260, 352
HspAI GCGC 1 cut(s) 17
KflI GGGWCCC 1 cut(s) 397
Ksp22I TGATCA 1 cut(s) 253
KspI CCGCGG 1 cut(s) 404
Kzo9I GATC 2 cut(s) 253, 289
LmnI GCTCC 3 cut(s) 44, 173, 355
LpnPI CCDG 7 cut(s) 5, 35, 103, 130, 238, 245, 379
Lsp1109I GCAGC 2 cut(s) 35, 257
LweI GCATC 1 cut(s) 454
MaeI CTAG 1 cut(s) 207
MalI GATC 2 cut(s) 255, 291
MboI GATC 2 cut(s) 253, 289
MfeI CAATTG 1 cut(s) 11
MhlI GDGCHC 1 cut(s) 49
MlsI TGGCCA 1 cut(s) 231
MluCI AATT 4 cut(s) 11, 72, 141, 275
MluNI TGGCCA 1 cut(s) 231
MnlI CCTC 4 cut(s) 193, 232, 258, 277
Mox20I TGGCCA 1 cut(s) 231
MscI TGGCCA 1 cut(s) 231
MseI TTAA 1 cut(s) 71
MslI CAYNNNNRTG 1 cut(s) 168
Msp20I TGGCCA 1 cut(s) 231
MspA1I CMGCKG 1 cut(s) 403
MspI CCGG 1 cut(s) 225
MspR9I CCNGG 1 cut(s) 118
MunI CAATTG 1 cut(s) 11
Mva1269I GAATGC 2 cut(s) 272, 358
MvaI CCWGG 1 cut(s) 118
MvnI CGCG 2 cut(s) 159, 403
MwoI GCNNNNNNNGC 3 cut(s) 23, 159, 456
NdeII GATC 2 cut(s) 253, 289
NlaIII CATG 3 cut(s) 114, 260, 352
NlaIV GGNNCC 4 cut(s) 54, 398, 399, 407
PagI TCATGA 2 cut(s) 256, 348
PctI GAATGC 2 cut(s) 272, 358
Pfl23II CGTACG 1 cut(s) 421
PkrI GCNGC 3 cut(s) 25, 161, 272
PpuMI RGGWCCY 2 cut(s) 397, 406
Psp5II RGGWCCY 2 cut(s) 397, 406
Psp6I CCWGG 1 cut(s) 116
PspGI CCWGG 1 cut(s) 116
PspLI CGTACG 1 cut(s) 421
PspN4I GGNNCC 4 cut(s) 54, 398, 399, 407
PspPI GGNCC 3 cut(s) 114, 397, 406
PspPPI RGGWCCY 2 cut(s) 397, 406
PstNI CAGNNNCTG 1 cut(s) 32
RsaI GTAC 3 cut(s) 392, 418, 423
RsaNI GTAC 3 cut(s) 391, 417, 422
RseI CAYNNNNRTG 1 cut(s) 168
SacII CCGCGG 1 cut(s) 404
SaqAI TTAA 1 cut(s) 71
SatI GCNGC 3 cut(s) 24, 160, 271
Sau3AI GATC 2 cut(s) 253, 289
Sau96I GGNCC 3 cut(s) 114, 397, 406
ScrFI CCNGG 1 cut(s) 118
SduI GDGCHC 1 cut(s) 49
SetI ASST 4 cut(s) 178, 224, 348, 388
SfaNI GCATC 1 cut(s) 454
Sfr303I CCGCGG 1 cut(s) 404
SgrBI CCGCGG 1 cut(s) 404
SinI GGWCC 3 cut(s) 114, 397, 406
SmiMI CAYNNNNRTG 1 cut(s) 168
Sse9I AATT 4 cut(s) 11, 72, 141, 275
SsiI CCGC 4 cut(s) 121, 159, 401, 403
SspMI CTAG 1 cut(s) 207
StyD4I CCNGG 1 cut(s) 116
StyI CCWWGG 1 cut(s) 206
TaaI ACNGT 4 cut(s) 203, 339, 421, 432
TasI AATT 4 cut(s) 11, 72, 141, 275
TauI GCSGC 1 cut(s) 162
Tru1I TTAA 1 cut(s) 71
Tru9I TTAA 1 cut(s) 71
TscAI CASTG 2 cut(s) 54, 437
TseI GCWGC 2 cut(s) 23, 270
TspDTI ATGAA 1 cut(s) 273
TspGWI ACGGA 1 cut(s) 342
TspRI CASTG 2 cut(s) 54, 437
VpaK11BI GGWCC 3 cut(s) 114, 397, 406
XmaJI CCTAGG 1 cut(s) 206
XspI CTAG 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.