Rw4G024390

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0016311
Physical Location & Seq
Forward (+)
50213624 .. 50215004
1381 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G024390.1

Sequence Viewer

Length: 882 bp
ATGATCGGTCCACCTTGGAATCTCATGATGGAGCAGTCTATTCCTTCCTTGACATACAAAGCTTGGTATCGTGATCCACCGTTAGGCCCGACACAGTACCGTACTAAAACAGTTTTCGAAAATGTATCACCAGAGGTATTGAGGGATTTCTTCTGGGATGATGAATTCAGACCCAGGTGGGATAAGACGCTCATTCACACCAAGGTTCTAAGCGTTTGCCTCCAAACTGGTACCATGATTGTTCATTGGATTAGAAAGCTACCACTACTCTGCTGCAATCGAGAATATATCATAATCCGTCGCATTTTTGAATCTGCATCAGGGTATTATTGCGTGACAAAGGCAACACCATATCCATCCATACCAAGGCTAAGAAAACCAAAACGAGTAGATGTCTATCATTCAAGTTGGCATATAAGGCCAGTGAAACCCAGGAATGCAGATCAGCAAATGGGATCAGAAGTGCTTCTATTTCACTACGAAGACATACGTCTCCCAAGAGAGGTTGTCAAAATGGGCGTCAGGGCAGGAATGTGGAACATGGTAAAGAAACTACATTTAGGAGTTCAGGTTTACAGTATGGAAAAAGCAAATGATGAATCTCCTTCATACCATGCAATTATGGCACACATAACTACAAAGCTTCCGGCTTCACCATCCACAATCATGTATCACATCCCAGAAGTTGAAGCAGATGAGAATGCATGCCAGAGCCAAAACCCACAAGAACAAGTAACAGAGTTCATAGTTCAGAATTCAGACCCAAATAAGACGCTCATTCACACCAAGGTTCTAAGCGTTTGCCTCCAAAAGTTCATTGGATTAGAAAGGTGCCATTTCCTAGTTTATAAGTACTTGCTAGAACAATTGAACTTCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

293

Amino Acids

34.36

Weight (kDa)

8.96

Isoelectric Point (pI)

49.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 31 - 184 5.7e-08 START domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016311)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g22000 FvH4_4g22000 FvH4_4g22000
malus_domestica MD13G1135100.v1.1
prunus_persica Prupe.1G210200_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0428201
rosa_laevigata RLG00000007170
rosa_multiflora Rmu_sc0004940.1_g000004 Rmu_sc0009599.1_g000003
rosa_rugosa Rorug04G0223600
rosa_samantha Rh4AG280800 Rh4BG286300 Rh4CG302000 Rh4DG283300
rosa_wichuraiana Rw4G024390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 849
Acc65I GGTACC 1 cut(s) 230
AccB1I GGYRCC 2 cut(s) 230, 831
AclWI GGATC 2 cut(s) 68, 463
AcsI RAATTY 2 cut(s) 164, 754
AcyI GRCGYC 1 cut(s) 519
AfaI GTAC 4 cut(s) 98, 103, 232, 854
AfiI CCNNNNNNNGG 3 cut(s) 83, 366, 502
AgsI TTSAA 4 cut(s) 311, 405, 689, 871
AjnI CCWGG 2 cut(s) 173, 431
AluBI AGCT 3 cut(s) 62, 259, 643
AluI AGCT 3 cut(s) 62, 259, 643
Alw26I GTCTC 1 cut(s) 497
AlwI GGATC 2 cut(s) 68, 463
AoxI GGCC 2 cut(s) 85, 419
ApeKI GCWGC 1 cut(s) 273
ApoI RAATTY 2 cut(s) 164, 754
ArsI GACNNNNNNTTYG 2 cut(s) 504, 536
Asp700I GAANNNNTTC 1 cut(s) 465
Asp718I GGTACC 1 cut(s) 230
AspS9I GGNCC 2 cut(s) 8, 86
AsuHPI GGTGA 2 cut(s) 120, 645
AsuII TTCGAA 1 cut(s) 117
AvaII GGWCC 1 cut(s) 8
BanI GGYRCC 2 cut(s) 230, 831
BbsI GAAGAC 1 cut(s) 489
BbvI GCAGC 1 cut(s) 260
BccI CCATC 3 cut(s) 22, 364, 664
BciT130I CCWGG 2 cut(s) 175, 433
BcoDI GTCTC 1 cut(s) 497
BfaI CTAG 2 cut(s) 842, 860
BisI GCNGC 1 cut(s) 274
BlsI GCNGC 1 cut(s) 275
BmcAI AGTACT 1 cut(s) 854
Bme1390I CCNGG 2 cut(s) 175, 433
Bme18I GGWCC 1 cut(s) 8
BmgT120I GGNCC 2 cut(s) 8, 86
BmiI GGNNCC 2 cut(s) 232, 833
BmrFI CCNGG 2 cut(s) 175, 433
BmsI GCATC 1 cut(s) 326
BoxI GACNNNNGTC 1 cut(s) 489
BpiI GAAGAC 1 cut(s) 489
Bpu14I TTCGAA 1 cut(s) 117
BsaBI GATNNNNATC 1 cut(s) 396
BsaHI GRCGYC 1 cut(s) 519
BsaJI CCNNGG 6 cut(s) 14, 173, 201, 365, 431, 786
BsaXI ACNNNNNCTCC 2 cut(s) 555, 585
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 366, 502
Bse1I ACTGG 2 cut(s) 232, 422
Bse8I GATNNNNATC 1 cut(s) 396
BseBI CCWGG 2 cut(s) 175, 433
BseDI CCNNGG 6 cut(s) 14, 173, 201, 365, 431, 786
BseGI GGATG 4 cut(s) 163, 356, 656, 675
BseJI GATNNNNATC 1 cut(s) 396
BseLI CCNNNNNNNGG 3 cut(s) 83, 366, 502
BseNI ACTGG 2 cut(s) 232, 422
BseXI GCAGC 1 cut(s) 260
BshFI GGCC 2 cut(s) 87, 421
BshNI GGYRCC 2 cut(s) 230, 831
BsiSI CCGG 1 cut(s) 647
BslI CCNNNNNNNGG 3 cut(s) 83, 366, 502
BsmAI GTCTC 1 cut(s) 497
BsmBI CGTCTC 1 cut(s) 497
BsmI GAATGC 2 cut(s) 442, 706
BsnI GGCC 2 cut(s) 87, 421
Bsp119I TTCGAA 1 cut(s) 117
Bsp143I GATC 4 cut(s) 3, 73, 442, 455
BspANI GGCC 2 cut(s) 87, 421
BspHI TCATGA 1 cut(s) 24
BspLI GGNNCC 2 cut(s) 232, 833
BspPI GGATC 2 cut(s) 68, 463
BspT104I TTCGAA 1 cut(s) 117
BspT107I GGYRCC 2 cut(s) 230, 831
BsrI ACTGG 2 cut(s) 232, 422
BssECI CCNNGG 6 cut(s) 14, 173, 201, 365, 431, 786
BssMI GATC 4 cut(s) 3, 73, 442, 455
BssNI GRCGYC 1 cut(s) 519
BssT1I CCWWGG 4 cut(s) 14, 201, 365, 786
Bst2UI CCWGG 2 cut(s) 175, 433
Bst4CI ACNGT 5 cut(s) 81, 96, 101, 112, 578
BstACI GRCGYC 1 cut(s) 519
BstBI TTCGAA 1 cut(s) 117
BstC8I GCNNGC 1 cut(s) 706
BstDEI CTNAG 3 cut(s) 209, 371, 794
BstF5I GGATG 4 cut(s) 163, 356, 656, 675
BstKTI GATC 4 cut(s) 6, 76, 445, 458
BstMAI GTCTC 1 cut(s) 497
BstMBI GATC 4 cut(s) 3, 73, 442, 455
BstMWI GCNNNNNNNGC 2 cut(s) 418, 623
BstNI CCWGG 2 cut(s) 175, 433
BstNSI RCATGY 1 cut(s) 708
BstPAI GACNNNNGTC 1 cut(s) 489
BstSCI CCNGG 2 cut(s) 173, 431
BstV1I GCAGC 1 cut(s) 260
BstV2I GAAGAC 1 cut(s) 489
BsuRI GGCC 2 cut(s) 87, 421
BtsCI GGATG 4 cut(s) 163, 356, 656, 675
BtsIMutI CAGTG 1 cut(s) 429
Cac8I GCNNGC 1 cut(s) 706
CciI TCATGA 1 cut(s) 24
Cfr13I GGNCC 2 cut(s) 8, 86
CseI GACGC 3 cut(s) 196, 508, 781
Csp6I GTAC 4 cut(s) 97, 102, 231, 853
CviAII CATG 6 cut(s) 25, 235, 541, 614, 667, 705
CviJI RGCY 8 cut(s) 62, 87, 259, 370, 421, 643, 650, 714
CviKI_1 RGCY 8 cut(s) 62, 87, 259, 370, 421, 643, 650, 714
CviQI GTAC 4 cut(s) 97, 102, 231, 853
DdeI CTNAG 3 cut(s) 209, 371, 794
DpnI GATC 4 cut(s) 5, 75, 444, 457
DpnII GATC 4 cut(s) 3, 73, 442, 455
Eco130I CCWWGG 4 cut(s) 14, 201, 365, 786
Eco47I GGWCC 1 cut(s) 8
EcoRI GAATTC 2 cut(s) 164, 754
EcoRII CCWGG 2 cut(s) 173, 431
EcoT14I CCWWGG 4 cut(s) 14, 201, 365, 786
EcoT22I ATGCAT 1 cut(s) 706
ErhI CCWWGG 4 cut(s) 14, 201, 365, 786
Esp3I CGTCTC 1 cut(s) 497
FaeI CATG 6 cut(s) 28, 238, 544, 617, 670, 708
FatI CATG 6 cut(s) 24, 234, 540, 613, 666, 704
Fnu4HI GCNGC 1 cut(s) 274
FokI GGATG 4 cut(s) 170, 343, 643, 662
Fsp4HI GCNGC 1 cut(s) 274
FspBI CTAG 2 cut(s) 842, 860
GluI GCNGC 1 cut(s) 274
HaeIII GGCC 2 cut(s) 87, 421
HapII CCGG 1 cut(s) 647
HgaI GACGC 3 cut(s) 196, 508, 781
Hin1I GRCGYC 1 cut(s) 519
Hin1II CATG 6 cut(s) 28, 238, 544, 617, 670, 708
HindIII AAGCTT 2 cut(s) 60, 641
HinfI GANTC 3 cut(s) 19, 311, 599
HpaII CCGG 1 cut(s) 647
HphI GGTGA 2 cut(s) 120, 645
Hpy166II GTNNAC 2 cut(s) 11, 574
Hpy188I TCNGA 4 cut(s) 170, 460, 753, 760
Hpy188III TCNNGA 3 cut(s) 25, 71, 281
Hpy8I GTNNAC 2 cut(s) 11, 574
Hpy99I CGWCG 1 cut(s) 303
HpyAV CCTTC 2 cut(s) 54, 615
HpyCH4III ACNGT 5 cut(s) 81, 96, 101, 112, 578
HpyCH4IV ACGT 1 cut(s) 490
HpyCH4V TGCA 5 cut(s) 276, 317, 440, 617, 704
HpyF10VI GCNNNNNNNGC 2 cut(s) 418, 623
HpyF3I CTNAG 3 cut(s) 209, 371, 794
HpySE526I ACGT 1 cut(s) 490
Hsp92I GRCGYC 1 cut(s) 519
Hsp92II CATG 6 cut(s) 28, 238, 544, 617, 670, 708
KpnI GGTACC 1 cut(s) 234
Kzo9I GATC 4 cut(s) 3, 73, 442, 455
LmnI GCTCC 1 cut(s) 31
Lsp1109I GCAGC 1 cut(s) 260
LweI GCATC 1 cut(s) 326
MaeI CTAG 2 cut(s) 842, 860
MaeII ACGT 1 cut(s) 490
MaeIII GTNAC 2 cut(s) 334, 733
MalI GATC 4 cut(s) 5, 75, 444, 457
MboI GATC 4 cut(s) 3, 73, 442, 455
MboII GAAGA 2 cut(s) 142, 494
MfeI CAATTG 1 cut(s) 866
MluCI AATT 4 cut(s) 164, 618, 754, 866
MnlI CCTC 5 cut(s) 127, 135, 230, 496, 815
Mph1103I ATGCAT 1 cut(s) 706
MroXI GAANNNNTTC 1 cut(s) 465
MslI CAYNNNNRTG 1 cut(s) 665
MspI CCGG 1 cut(s) 647
MspR9I CCNGG 2 cut(s) 175, 433
MunI CAATTG 1 cut(s) 866
Mva1269I GAATGC 2 cut(s) 442, 706
MvaI CCWGG 2 cut(s) 175, 433
MwoI GCNNNNNNNGC 2 cut(s) 418, 623
NdeII GATC 4 cut(s) 3, 73, 442, 455
NlaIII CATG 6 cut(s) 28, 238, 544, 617, 670, 708
NlaIV GGNNCC 2 cut(s) 232, 833
NmuCI GTSAC 1 cut(s) 334
NsiI ATGCAT 1 cut(s) 706
NspI RCATGY 1 cut(s) 708
NspV TTCGAA 1 cut(s) 117
PaeI GCATGC 1 cut(s) 708
PagI TCATGA 1 cut(s) 24
PctI GAATGC 2 cut(s) 442, 706
PdmI GAANNNNTTC 1 cut(s) 465
PfeI GAWTC 3 cut(s) 19, 311, 599
PkrI GCNGC 1 cut(s) 275
PshAI GACNNNNGTC 1 cut(s) 489
PsiI TTATAA 1 cut(s) 849
Psp6I CCWGG 2 cut(s) 173, 431
PspGI CCWGG 2 cut(s) 173, 431
PspN4I GGNNCC 2 cut(s) 232, 833
PspPI GGNCC 2 cut(s) 8, 86
RsaI GTAC 4 cut(s) 98, 103, 232, 854
RsaNI GTAC 4 cut(s) 97, 102, 231, 853
RseI CAYNNNNRTG 1 cut(s) 665
SatI GCNGC 1 cut(s) 274
Sau3AI GATC 4 cut(s) 3, 73, 442, 455
Sau96I GGNCC 2 cut(s) 8, 86
ScaI AGTACT 1 cut(s) 854
ScrFI CCNGG 2 cut(s) 175, 433
SfaNI GCATC 1 cut(s) 326
SfuI TTCGAA 1 cut(s) 117
SinI GGWCC 1 cut(s) 8
SmiMI CAYNNNNRTG 1 cut(s) 665
SphI GCATGC 1 cut(s) 708
Sse9I AATT 4 cut(s) 164, 618, 754, 866
SspMI CTAG 2 cut(s) 842, 860
StyD4I CCNGG 2 cut(s) 173, 431
StyI CCWWGG 4 cut(s) 14, 201, 365, 786
TaaI ACNGT 5 cut(s) 81, 96, 101, 112, 578
TaiI ACGT 1 cut(s) 493
TaqI TCGA 2 cut(s) 117, 280
TasI AATT 4 cut(s) 164, 618, 754, 866
TatI WGTACW 1 cut(s) 852
TfiI GAWTC 3 cut(s) 19, 311, 599
TscAI CASTG 1 cut(s) 429
TseFI GTSAC 1 cut(s) 334
TseI GCWGC 1 cut(s) 273
Tsp45I GTSAC 1 cut(s) 334
TspDTI ATGAA 6 cut(s) 177, 233, 597, 612, 733, 805
TspGWI ACGGA 1 cut(s) 287
TspRI CASTG 1 cut(s) 429
VpaK11BI GGWCC 1 cut(s) 8
XapI RAATTY 2 cut(s) 164, 754
XceI RCATGY 1 cut(s) 708
XcmI CCANNNNNNNNNTGG 1 cut(s) 815
XmnI GAANNNNTTC 1 cut(s) 465
XspI CTAG 2 cut(s) 842, 860
ZrmI AGTACT 1 cut(s) 854
Zsp2I ATGCAT 1 cut(s) 706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.