FvH4_4g23370

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014177
Physical Location & Seq
Reverse (-)
25869847 .. 25873052
3206 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g23370.t2

Sequence Viewer

Length: 1053 bp
ATGGGGAAGCTACTATGTGATTCCACCACCGTGGCCGAGTCATTCCAGACCTCCTCCCCCACCGTCGCCTGGCGGGACCCGCCCCCGAAGTCGGCGCCGCTCCATCAGGACGACGACGACCACCGAACCGAGCCCCTCGATCTCATCGACCAGTCAACGGACGACGCGTGGGGAGCTGTCGTCGGCCTGGAGGAGCAGCAGCGGCGCCACCTCCAGCGGCTTCACGCCAAGGGGGTCTTGTGGAAGCATCCGGAGGCGGACGGCGAATCCTCGGTGGTTTTCAGGCTGTCGCACGGCGGGGAGGTGTCGTCCGACGGGAACTGTTTGTTTACGGCGTCGCAGAAGGCGATGAAGGCGGCGCGTGAGGTCGACGCGCGGGAGCTGAGGCGGCGGACGGTGCGGAGGTTCATGGAGGATTTCGGATCTGCGGGAGAGGAGGAGAGGGACGTCATCAACGACGTCATTCGGCATCTCTACGCGCCTGATCTGAAGGCCGGGTGGGGGATACACGTGATTCAGGAGGTCAAGCTTTTGGCCAAGAAAGACGAGCGCGTGAGTCTTGACTCTGCTATTGACGAGCTGCTTCAGCTGGGAATGCAGAGGGAGCTGGCGGCGGAGTCTATTTACAAAGAGAGATGTATCTCAGTGAGTGATGGACCTAGTTGGGCGAAATACATGTTGATCTCTGGTTCCCCGGAGGATGAATATGATATCATCACATTGCAATACACAGAGGAAGGCTTGCTAACAATCGATGAAAATAGAGAAGGTCATGCCGCGGCTTTTGGAGATGATATAGCAATCGAGTGTCTTGCAACAGAGTTTAAGCGAGAGATTTATGTGGTTCAAGCACACGGAGCAGATGCAATGGTTGATGAGGAAAATTGTGTTTTCTTCCTTCCGCACCGACCAAGGACTCAGATTTGTGAACCACCCTTCTTTCTTTTCATGAAAGGAACAGGTTGGTGTGGTGCTGGAGCTGATCACTATGAACCCCTGATTGCTCATCCCTTGTCCTTTGTTTCTCAGGAGAAGGTCGCTATGGTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

351

Amino Acids

39.22

Weight (kDa)

4.87

Isoelectric Point (pI)

48.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13100
fragaria_vesca FvH4_4g23370 FvH4_4g23370
malus_domestica MD16G1123300.v1.1
prunus_persica Prupe.1G222300_v2.0.a1 Prupe.1G222300_v2.0.a1
pyrus_communis pycom16g10550
rosa_chinensis RchiOBHm_Chr4g0429761
rosa_laevigata RLG00000007045
rosa_multiflora Rmu_sc0001935.1_g000009
rosa_roxburghii Rroxscaffold_5G00371530
rosa_rugosa Rorug04G0237900
rosa_samantha Rh4AG294300 Rh4BG300300 Rh4CG315900 Rh4DG297400
rosa_wichuraiana Rw4G025460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 450, 462
AccB1I GGYRCC 2 cut(s) 94, 204
AccBSI CCGCTC 1 cut(s) 100
AccI GTMKAC 1 cut(s) 369
AccII CGCG 7 cut(s) 167, 361, 374, 376, 479, 552, 779
AccIII TCCGGA 1 cut(s) 250
AclWI GGATC 1 cut(s) 430
AcoI YGGCCR 2 cut(s) 33, 534
AcuI CTGAAG 2 cut(s) 509, 569
AcvI CACGTG 1 cut(s) 511
AcyI GRCGYC 5 cut(s) 95, 205, 335, 447, 459
AfiI CCNNNNNNNGG 4 cut(s) 69, 91, 157, 501
AflIII ACRYGT 3 cut(s) 165, 508, 675
AgsI TTSAA 1 cut(s) 848
AjnI CCWGG 2 cut(s) 68, 186
AleI CACNNNNGTG 1 cut(s) 29
AluBI AGCT 8 cut(s) 10, 176, 382, 529, 580, 589, 607, 980
AluI AGCT 8 cut(s) 10, 176, 382, 529, 580, 589, 607, 980
AlwI GGATC 1 cut(s) 430
Aor13HI TCCGGA 1 cut(s) 250
AoxI GGCC 4 cut(s) 33, 184, 492, 534
ApeKI GCWGC 3 cut(s) 196, 199, 580
AspLEI GCGC 6 cut(s) 97, 207, 361, 376, 481, 552
AspS9I GGNCC 2 cut(s) 76, 656
AsuC2I CCSGG 2 cut(s) 496, 695
AvaII GGWCC 2 cut(s) 76, 656
BalI TGGCCA 1 cut(s) 536
BanI GGYRCC 2 cut(s) 94, 204
BanII GRGCYC 1 cut(s) 135
BbrPI CACGTG 1 cut(s) 511
BbvCI CCTCAGC 1 cut(s) 383
BbvI GCAGC 3 cut(s) 208, 211, 567
BccI CCATC 2 cut(s) 111, 647
BceAI ACGGC 3 cut(s) 277, 310, 348
BcgI CGANNNNNNTGC 2 cut(s) 794, 828
BciT130I CCWGG 2 cut(s) 70, 188
BciVI GTATCC 1 cut(s) 498
BclI TGATCA 1 cut(s) 982
BcnI CCSGG 2 cut(s) 496, 695
BfaI CTAG 1 cut(s) 660
BfoI RGCGCY 2 cut(s) 98, 208
BfuI GTATCC 1 cut(s) 498
Bme1390I CCNGG 4 cut(s) 70, 188, 496, 695
Bme18I GGWCC 2 cut(s) 76, 656
BmgT120I GGNCC 2 cut(s) 76, 656
BmiI GGNNCC 5 cut(s) 77, 78, 96, 206, 691
BmrFI CCNGG 4 cut(s) 70, 188, 496, 695
BmsI GCATC 3 cut(s) 256, 478, 853
BpmI CTGGAG 3 cut(s) 197, 209, 996
Bpu10I CCTNAGC 1 cut(s) 383
BpuMI CCSGG 2 cut(s) 496, 695
Bsa29I ATCGAT 1 cut(s) 753
BsaAI YACGTR 1 cut(s) 511
BsaHI GRCGYC 5 cut(s) 95, 205, 335, 447, 459
BsaJI CCNNGG 6 cut(s) 30, 228, 270, 693, 777, 911
BsaWI WCCGGW 1 cut(s) 250
BsaXI ACNNNNNCTCC 4 cut(s) 165, 195, 293, 323
Bsc4I CCNNNNNNNGG 4 cut(s) 69, 91, 157, 501
Bse1I ACTGG 1 cut(s) 151
Bse3DI GCAATG 2 cut(s) 719, 873
BseAI TCCGGA 1 cut(s) 250
BseBI CCWGG 2 cut(s) 70, 188
BseCI ATCGAT 1 cut(s) 753
BseDI CCNNGG 6 cut(s) 30, 228, 270, 693, 777, 911
BseGI GGATG 3 cut(s) 247, 706, 1006
BseLI CCNNNNNNNGG 4 cut(s) 69, 91, 157, 501
BseMI GCAATG 2 cut(s) 719, 873
BseMII CTCAG 4 cut(s) 374, 657, 932, 1040
BseNI ACTGG 1 cut(s) 151
BseRI GAGGAG 4 cut(s) 43, 206, 449, 452
BseXI GCAGC 3 cut(s) 208, 211, 567
BseYI CCCAGC 1 cut(s) 589
Bsh1236I CGCG 7 cut(s) 167, 361, 374, 376, 479, 552, 779
BshFI GGCC 4 cut(s) 35, 186, 494, 536
BshNI GGYRCC 2 cut(s) 94, 204
BshVI ATCGAT 1 cut(s) 753
BsiSI CCGG 3 cut(s) 251, 495, 695
BslFI GGGAC 2 cut(s) 89, 458
BslI CCNNNNNNNGG 4 cut(s) 69, 91, 157, 501
BsmFI GGGAC 2 cut(s) 89, 458
BsmI GAATGC 1 cut(s) 600
BsnI GGCC 4 cut(s) 35, 186, 494, 536
Bsp1286I GDGCHC 1 cut(s) 135
Bsp13I TCCGGA 1 cut(s) 250
Bsp143I GATC 5 cut(s) 139, 422, 484, 681, 982
BspANI GGCC 4 cut(s) 35, 186, 494, 536
BspCNI CTCAG 4 cut(s) 375, 656, 931, 1039
BspDI ATCGAT 1 cut(s) 753
BspEI TCCGGA 1 cut(s) 250
BspFNI CGCG 7 cut(s) 167, 361, 374, 376, 479, 552, 779
BspHI TCATGA 1 cut(s) 948
BspLI GGNNCC 5 cut(s) 77, 78, 96, 206, 691
BspPI GGATC 1 cut(s) 430
BspT107I GGYRCC 2 cut(s) 94, 204
BsrBI CCGCTC 1 cut(s) 100
BsrDI GCAATG 2 cut(s) 719, 873
BsrI ACTGG 1 cut(s) 151
BssECI CCNNGG 6 cut(s) 30, 228, 270, 693, 777, 911
BssMI GATC 5 cut(s) 139, 422, 484, 681, 982
BssNI GRCGYC 5 cut(s) 95, 205, 335, 447, 459
BssT1I CCWWGG 2 cut(s) 228, 911
Bst2UI CCWGG 2 cut(s) 70, 188
Bst4CI ACNGT 4 cut(s) 31, 64, 323, 397
BstACI GRCGYC 5 cut(s) 95, 205, 335, 447, 459
BstBAI YACGTR 1 cut(s) 511
BstC8I GCNNGC 2 cut(s) 609, 743
BstDEI CTNAG 4 cut(s) 383, 643, 918, 1026
BstDSI CCRYGG 2 cut(s) 30, 777
BstF5I GGATG 3 cut(s) 247, 706, 1006
BstFNI CGCG 7 cut(s) 167, 361, 374, 376, 479, 552, 779
BstH2I RGCGCY 2 cut(s) 98, 208
BstHHI GCGC 6 cut(s) 97, 207, 361, 376, 481, 552
BstKTI GATC 5 cut(s) 142, 425, 487, 684, 985
BstMBI GATC 5 cut(s) 139, 422, 484, 681, 982
BstNI CCWGG 2 cut(s) 70, 188
BstNSI RCATGY 1 cut(s) 679
BstSCI CCNGG 4 cut(s) 68, 186, 494, 693
BstUI CGCG 7 cut(s) 167, 361, 374, 376, 479, 552, 779
BstV1I GCAGC 3 cut(s) 208, 211, 567
BstX2I RGATCY 1 cut(s) 422
BstXI CCANNNNNNTGG 1 cut(s) 31
BstYI RGATCY 1 cut(s) 422
Bsu15I ATCGAT 1 cut(s) 753
BsuI GTATCC 1 cut(s) 498
BsuRI GGCC 4 cut(s) 35, 186, 494, 536
BsuTUI ATCGAT 1 cut(s) 753
BtgI CCRYGG 2 cut(s) 30, 777
BtgZI GCGATG 1 cut(s) 362
BtsCI GGATG 3 cut(s) 247, 706, 1006
BtsIMutI CAGTG 1 cut(s) 651
Cac8I GCNNGC 2 cut(s) 609, 743
CciI TCATGA 1 cut(s) 948
CfoI GCGC 6 cut(s) 97, 207, 361, 376, 481, 552
Cfr13I GGNCC 2 cut(s) 76, 656
Cfr42I CCGCGG 1 cut(s) 780
ClaI ATCGAT 1 cut(s) 753
CseI GACGC 3 cut(s) 173, 324, 380
CviAII CATG 4 cut(s) 409, 676, 773, 949
DdeI CTNAG 4 cut(s) 383, 643, 918, 1026
DinI GGCGCC 2 cut(s) 96, 206
DpnI GATC 5 cut(s) 141, 424, 486, 683, 984
DpnII GATC 5 cut(s) 139, 422, 484, 681, 982
EaeI YGGCCR 2 cut(s) 33, 534
EciI GGCGGA 3 cut(s) 272, 406, 629
Eco130I CCWWGG 2 cut(s) 228, 911
Eco24I GRGCYC 1 cut(s) 135
Eco32I GATATC 1 cut(s) 712
Eco47I GGWCC 2 cut(s) 76, 656
Eco57I CTGAAG 2 cut(s) 509, 569
Eco72I CACGTG 1 cut(s) 511
EcoO109I RGGNCCY 1 cut(s) 76
EcoRII CCWGG 2 cut(s) 68, 186
EcoRV GATATC 1 cut(s) 712
EcoT14I CCWWGG 2 cut(s) 228, 911
EcoT38I GRGCYC 1 cut(s) 135
EgeI GGCGCC 2 cut(s) 96, 206
EheI GGCGCC 2 cut(s) 96, 206
ErhI CCWWGG 2 cut(s) 228, 911
FaeI CATG 4 cut(s) 412, 679, 776, 952
FalI AAGNNNNNCTT 2 cut(s) 221, 253
FaqI GGGAC 2 cut(s) 89, 458
FatI CATG 4 cut(s) 408, 675, 772, 948
FauI CCCGC 5 cut(s) 66, 87, 290, 369, 421
FbaI TGATCA 1 cut(s) 982
FblI GTMKAC 1 cut(s) 369
FokI GGATG 3 cut(s) 234, 713, 993
FriOI GRGCYC 1 cut(s) 135
FspBI CTAG 1 cut(s) 660
GlaI GCGC 6 cut(s) 96, 206, 360, 375, 480, 551
GsaI CCCAGC 1 cut(s) 593
GsuI CTGGAG 3 cut(s) 197, 209, 996
HaeII RGCGCY 2 cut(s) 98, 208
HaeIII GGCC 4 cut(s) 35, 186, 494, 536
HapII CCGG 3 cut(s) 251, 495, 695
HgaI GACGC 3 cut(s) 173, 324, 380
HhaI GCGC 6 cut(s) 97, 207, 361, 376, 481, 552
Hin1I GRCGYC 5 cut(s) 95, 205, 335, 447, 459
Hin1II CATG 4 cut(s) 412, 679, 776, 952
Hin6I GCGC 6 cut(s) 95, 205, 359, 374, 479, 550
HinP1I GCGC 6 cut(s) 95, 205, 359, 374, 479, 550
HincII GTYRAC 2 cut(s) 156, 370
HindII GTYRAC 2 cut(s) 156, 370
HindIII AAGCTT 1 cut(s) 527
HinfI GANTC 8 cut(s) 20, 38, 266, 514, 556, 563, 617, 916
HpaII CCGG 3 cut(s) 251, 495, 695
Hpy166II GTNNAC 4 cut(s) 156, 330, 370, 929
Hpy188I TCNGA 4 cut(s) 313, 422, 489, 921
Hpy188III TCNNGA 7 cut(s) 46, 107, 251, 518, 560, 949, 1028
Hpy8I GTNNAC 4 cut(s) 156, 330, 370, 929
Hpy99I CGWCG 9 cut(s) 68, 116, 119, 167, 185, 317, 340, 374, 461
HpyAV CCTTC 8 cut(s) 337, 346, 484, 731, 761, 908, 946, 1027
HpyCH4III ACNGT 4 cut(s) 31, 64, 323, 397
HpyCH4IV ACGT 3 cut(s) 447, 459, 510
HpyCH4V TGCA 4 cut(s) 598, 724, 815, 866
HpyF3I CTNAG 4 cut(s) 383, 643, 918, 1026
HpySE526I ACGT 3 cut(s) 447, 459, 510
Hsp92I GRCGYC 5 cut(s) 95, 205, 335, 447, 459
Hsp92II CATG 4 cut(s) 412, 679, 776, 952
HspAI GCGC 6 cut(s) 95, 205, 359, 374, 479, 550
KasI GGCGCC 2 cut(s) 94, 204
KflI GGGWCCC 1 cut(s) 76
Kpn2I TCCGGA 1 cut(s) 250
Ksp22I TGATCA 1 cut(s) 982
KspI CCGCGG 1 cut(s) 780
Kzo9I GATC 5 cut(s) 139, 422, 484, 681, 982
LmnI GCTCC 7 cut(s) 105, 173, 193, 379, 604, 857, 977
Lsp1109I GCAGC 3 cut(s) 208, 211, 567
LweI GCATC 3 cut(s) 256, 478, 853
MaeI CTAG 1 cut(s) 660
MaeII ACGT 3 cut(s) 447, 459, 510
MalI GATC 5 cut(s) 141, 424, 486, 683, 984
MbiI CCGCTC 1 cut(s) 100
MboI GATC 5 cut(s) 139, 422, 484, 681, 982
MboII GAAGA 1 cut(s) 886
MflI RGATCY 1 cut(s) 422
MhlI GDGCHC 1 cut(s) 135
MlsI TGGCCA 1 cut(s) 536
MluCI AATT 1 cut(s) 883
MluI ACGCGT 1 cut(s) 165
MluNI TGGCCA 1 cut(s) 536
Mly113I GGCGCC 2 cut(s) 95, 205
MlyI GAGTC 5 cut(s) 47, 557, 565, 626, 910
MmeI TCCRAC 1 cut(s) 336
Mox20I TGGCCA 1 cut(s) 536
MroI TCCGGA 1 cut(s) 250
MscI TGGCCA 1 cut(s) 536
MseI TTAA 1 cut(s) 825
MslI CAYNNNNRTG 1 cut(s) 29
Msp20I TGGCCA 1 cut(s) 536
MspA1I CMGCKG 4 cut(s) 202, 217, 589, 779
MspI CCGG 3 cut(s) 251, 495, 695
MspR9I CCNGG 4 cut(s) 70, 188, 496, 695
Mva1269I GAATGC 1 cut(s) 600
MvaI CCWGG 2 cut(s) 70, 188
MvnI CGCG 7 cut(s) 167, 361, 374, 376, 479, 552, 779
NarI GGCGCC 2 cut(s) 95, 205
NciI CCSGG 2 cut(s) 496, 695
NdeII GATC 5 cut(s) 139, 422, 484, 681, 982
NlaIII CATG 4 cut(s) 412, 679, 776, 952
NlaIV GGNNCC 5 cut(s) 77, 78, 96, 206, 691
NmeAIII GCCGAG 1 cut(s) 61
NspI RCATGY 1 cut(s) 679
OliI CACNNNNGTG 1 cut(s) 29
PagI TCATGA 1 cut(s) 948
PciI ACATGT 1 cut(s) 675
PcsI WCGNNNNNNNCGW 4 cut(s) 144, 164, 344, 453
PctI GAATGC 1 cut(s) 600
PfeI GAWTC 3 cut(s) 20, 266, 514
PleI GAGTC 5 cut(s) 46, 557, 564, 625, 910
PluTI GGCGCC 2 cut(s) 98, 208
PmaCI CACGTG 1 cut(s) 511
PmlI CACGTG 1 cut(s) 511
PpsI GAGTC 5 cut(s) 46, 557, 564, 625, 910
Ppu21I YACGTR 1 cut(s) 511
PpuMI RGGWCCY 1 cut(s) 76
PscI ACATGT 1 cut(s) 675
Psp5II RGGWCCY 1 cut(s) 76
Psp6I CCWGG 2 cut(s) 68, 186
PspCI CACGTG 1 cut(s) 511
PspFI CCCAGC 1 cut(s) 589
PspGI CCWGG 2 cut(s) 68, 186
PspN4I GGNNCC 5 cut(s) 77, 78, 96, 206, 691
PspPI GGNCC 2 cut(s) 76, 656
PspPPI RGGWCCY 1 cut(s) 76
PsuI RGATCY 1 cut(s) 422
PvuII CAGCTG 1 cut(s) 589
RseI CAYNNNNRTG 1 cut(s) 29
SacII CCGCGG 1 cut(s) 780
SalI GTCGAC 1 cut(s) 368
SaqAI TTAA 1 cut(s) 825
Sau3AI GATC 5 cut(s) 139, 422, 484, 681, 982
Sau96I GGNCC 2 cut(s) 76, 656
SchI GAGTC 5 cut(s) 47, 557, 565, 626, 910
ScrFI CCNGG 4 cut(s) 70, 188, 496, 695
SduI GDGCHC 1 cut(s) 135
SfaNI GCATC 3 cut(s) 256, 478, 853
SfoI GGCGCC 2 cut(s) 96, 206
Sfr303I CCGCGG 1 cut(s) 780
SgrBI CCGCGG 1 cut(s) 780
SinI GGWCC 2 cut(s) 76, 656
SmiMI CAYNNNNRTG 1 cut(s) 29
Sse9I AATT 1 cut(s) 883
SspDI GGCGCC 2 cut(s) 94, 204
SspMI CTAG 1 cut(s) 660
StyD4I CCNGG 4 cut(s) 68, 186, 494, 693
StyI CCWWGG 2 cut(s) 228, 911
TaaI ACNGT 4 cut(s) 31, 64, 323, 397
TaiI ACGT 3 cut(s) 450, 462, 513
TaqI TCGA 5 cut(s) 138, 147, 369, 753, 804
TasI AATT 1 cut(s) 883
TauI GCSGC 8 cut(s) 100, 205, 220, 359, 391, 614, 779, 782
TfiI GAWTC 3 cut(s) 20, 266, 514
Tru1I TTAA 1 cut(s) 825
Tru9I TTAA 1 cut(s) 825
TscAI CASTG 1 cut(s) 651
TseI GCWGC 3 cut(s) 196, 199, 580
TspDTI ATGAA 7 cut(s) 365, 397, 717, 771, 937, 965, 1005
TspGWI ACGGA 2 cut(s) 173, 870
TspRI CASTG 1 cut(s) 651
VpaK11BI GGWCC 2 cut(s) 76, 656
XceI RCATGY 1 cut(s) 679
XmiI GTMKAC 1 cut(s) 369
XspI CTAG 1 cut(s) 660
ZraI GACGTC 2 cut(s) 448, 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.