Prupe.1G222300_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0014177
Physical Location & Seq
Reverse (-)
23658879 .. 23661415
2537 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G222300.2

Sequence Viewer

Length: 1074 bp
ATGGGGAAACTACTATGCGATTCGACTACGGTTGCCGAGACATTTCAAAGCTCCTCGCCGACGGTTCCCTGGAGGGACCCACCCAAGGCGTCGCCACTGGACGAGGACGCAATCTCGGCCGTAGATCTCGTTGACCAGTCAACGGCCGACGTCGCATGGGACAGCGTGGTGGGCCTCGAGGAGCCGCAGAGACGGCACCTCCAGAGGCTGCACACGAAGGGCGTGCTGTGGAAGCACCCAGAGGATCAAGACTCGTCCAAGTCCGACTCGGACTCGTTAATGCCACAGAAGTCGATGGTCTTCAGACTGTCGCACGGGGGCGAGGTGTCGTCGGACGGGAATTGCCTGTTCACGGCGTCTCAGAAGGCGATGAGGGTGGCGCGTGAGGTGGACGCGCGGGAGTTGAGGAGGCGGACGGTCCGGAGGTTCACCGAGGACTACGGATCTGCGGCCGGGGAGGAGAAGCGCGTGATAGACGACGCTATTCGGCATATGTACGCGCCTGATCTGAGGGCTGGGTGGGGGATACATTTGATTCAGGAGGTCAAGTTGTTGGCCAAGAAAGACGAGCGCGTGAGTCTTGATTCTGCCATCGATGAGCTTCTTCAGCTTGGCATGCAGAGAGAGCTGGCAGCAGAGTCTATTTACAAAGAAAGATGTATCCCAGTGAATGATGGAGCGAGTTGGGCCAAATACATGTCGACCTCTGGTTCTACCCATGATGAATATGATATCATCACATTGCAGTACACAGAGGAAGGTTTATTATCCATTGATGAAAACAGAGAAGGTCATGCCGCTGCTTTTGGAGATGATATCGCAATAGAGTGTCTTGCCACAGAGTTTAAGCGAGAGATATATGTGGTTCAAGCACATGGATCGGATGCAATGGTTGATGAAGAAAATTGTGTTTTCTTCCTTCCACATCGACCCAGGAGTCAAATTTGTGAACCTCCTTTCTTTCTTTTCATGAAAGGAACAGGTTGGTGTGGTGCCGGAGCTGATCACTATGAACCCCTCATCGCCCATCCTCTGTCATTAATTTCCCAGGAGAAGGTTGCTGTGGTACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

358

Amino Acids

39.88

Weight (kDa)

4.98

Isoelectric Point (pI)

55.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13100
fragaria_vesca FvH4_4g23370 FvH4_4g23370
malus_domestica MD16G1123300.v1.1
prunus_persica Prupe.1G222300_v2.0.a1 Prupe.1G222300_v2.0.a1
pyrus_communis pycom16g10550
rosa_chinensis RchiOBHm_Chr4g0429761
rosa_laevigata RLG00000007045
rosa_multiflora Rmu_sc0001935.1_g000009
rosa_roxburghii Rroxscaffold_5G00371530
rosa_rugosa Rorug04G0237900
rosa_samantha Rh4AG294300 Rh4BG300300 Rh4CG315900 Rh4DG297400
rosa_wichuraiana Rw4G025460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 936
AatII GACGTC 1 cut(s) 153
AbsI CCTCGAGG 1 cut(s) 176
AccB1I GGYRCC 2 cut(s) 195, 992
AccI GTMKAC 1 cut(s) 701
AccII CGCG 6 cut(s) 382, 395, 397, 468, 500, 573
AccIII TCCGGA 1 cut(s) 420
AciI CCGC 5 cut(s) 185, 397, 412, 449, 798
AclWI GGATC 3 cut(s) 252, 451, 886
AcoI YGGCCR 4 cut(s) 117, 144, 450, 555
AcsI RAATTY 1 cut(s) 942
AcuI CTGAAG 2 cut(s) 286, 590
AcyI GRCGYC 3 cut(s) 89, 150, 356
AfaI GTAC 3 cut(s) 497, 749, 1068
AfiI CCNNNNNNNGG 4 cut(s) 85, 142, 352, 1054
AflIII ACRYGT 1 cut(s) 696
AgsI TTSAA 2 cut(s) 47, 869
AjnI CCWGG 3 cut(s) 68, 932, 1047
AluBI AGCT 5 cut(s) 51, 601, 610, 628, 1001
AluI AGCT 5 cut(s) 51, 601, 610, 628, 1001
Alw26I GTCTC 3 cut(s) 32, 184, 363
AlwI GGATC 3 cut(s) 252, 451, 886
AlwNI CAGNNNCTG 1 cut(s) 208
Ama87I CYCGRG 1 cut(s) 176
Aor13HI TCCGGA 1 cut(s) 420
AoxI GGCC 6 cut(s) 117, 144, 172, 450, 555, 687
ApeKI GCWGC 3 cut(s) 208, 632, 800
ApoI RAATTY 1 cut(s) 942
AseI ATTAAT 1 cut(s) 1040
AspLEI GCGC 5 cut(s) 382, 397, 468, 502, 573
AspS9I GGNCC 4 cut(s) 76, 172, 418, 687
AsuC2I CCSGG 1 cut(s) 454
AsuHPI GGTGA 1 cut(s) 421
AvaI CYCGRG 1 cut(s) 176
AvaII GGWCC 2 cut(s) 76, 418
BalI TGGCCA 1 cut(s) 557
BanI GGYRCC 2 cut(s) 195, 992
BbsI GAAGAC 1 cut(s) 292
BbvI GCAGC 3 cut(s) 195, 644, 787
BccI CCATC 4 cut(s) 289, 599, 668, 1035
BceAI ACGGC 4 cut(s) 104, 159, 209, 369
BcgI CGANNNNNNTGC 4 cut(s) 205, 239, 861, 895
BciT130I CCWGG 3 cut(s) 70, 934, 1049
BciVI GTATCC 2 cut(s) 519, 671
BclI TGATCA 1 cut(s) 1003
BcnI CCSGG 1 cut(s) 454
BcoDI GTCTC 3 cut(s) 32, 184, 363
BfuI GTATCC 2 cut(s) 519, 671
BglII AGATCT 1 cut(s) 124
BisI GCNGC 6 cut(s) 185, 209, 450, 633, 798, 801
BlsI GCNGC 6 cut(s) 186, 210, 451, 634, 799, 802
Bme1390I CCNGG 4 cut(s) 70, 454, 934, 1049
Bme18I GGWCC 2 cut(s) 76, 418
BmeT110I CYCGRG 1 cut(s) 176
BmgT120I GGNCC 4 cut(s) 76, 172, 418, 687
BmiI GGNNCC 6 cut(s) 66, 77, 78, 183, 197, 994
BmrFI CCNGG 4 cut(s) 70, 454, 934, 1049
BmrI ACTGGG 1 cut(s) 659
BmsI GCATC 1 cut(s) 874
BmuI ACTGGG 1 cut(s) 659
BpiI GAAGAC 1 cut(s) 292
BpmI CTGGAG 2 cut(s) 91, 185
BpuMI CCSGG 1 cut(s) 454
Bsa29I ATCGAT 1 cut(s) 594
BsaHI GRCGYC 3 cut(s) 89, 150, 356
BsaJI CCNNGG 6 cut(s) 68, 84, 432, 453, 932, 1047
BsaWI WCCGGW 1 cut(s) 420
BsaXI ACNNNNNCTCC 4 cut(s) 183, 213, 533, 563
Bsc4I CCNNNNNNNGG 4 cut(s) 85, 142, 352, 1054
Bse1I ACTGG 3 cut(s) 102, 136, 665
Bse3DI GCAATG 2 cut(s) 740, 894
BseAI TCCGGA 1 cut(s) 420
BseBI CCWGG 3 cut(s) 70, 934, 1049
BseCI ATCGAT 1 cut(s) 594
BseDI CCNNGG 6 cut(s) 68, 84, 432, 453, 932, 1047
BseGI GGATG 2 cut(s) 889, 1027
BseLI CCNNNNNNNGG 4 cut(s) 85, 142, 352, 1054
BseMI GCAATG 2 cut(s) 740, 894
BseMII CTCAG 2 cut(s) 374, 500
BseNI ACTGG 3 cut(s) 102, 136, 665
BseRI GAGGAG 4 cut(s) 43, 194, 421, 473
BseX3I CGGCCG 3 cut(s) 117, 144, 450
BseXI GCAGC 3 cut(s) 195, 644, 787
BseYI CCCAGC 1 cut(s) 515
BsgI GTGCAG 1 cut(s) 194
Bsh1236I CGCG 6 cut(s) 382, 395, 397, 468, 500, 573
Bsh1285I CGRYCG 3 cut(s) 120, 147, 453
BshFI GGCC 6 cut(s) 119, 146, 174, 452, 557, 689
BshNI GGYRCC 2 cut(s) 195, 992
BshVI ATCGAT 1 cut(s) 594
BsiEI CGRYCG 3 cut(s) 120, 147, 453
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 3 cut(s) 421, 453, 996
BslFI GGGAC 2 cut(s) 89, 173
BslI CCNNNNNNNGG 4 cut(s) 85, 142, 352, 1054
BsmAI GTCTC 3 cut(s) 32, 184, 363
BsmBI CGTCTC 2 cut(s) 184, 363
BsmFI GGGAC 2 cut(s) 89, 173
BsnI GGCC 6 cut(s) 119, 146, 174, 452, 557, 689
BsoBI CYCGRG 1 cut(s) 176
Bsp13I TCCGGA 1 cut(s) 420
Bsp143I GATC 6 cut(s) 124, 244, 443, 505, 878, 1003
BspACI CCGC 5 cut(s) 185, 397, 412, 449, 798
BspANI GGCC 6 cut(s) 119, 146, 174, 452, 557, 689
BspCNI CTCAG 2 cut(s) 373, 501
BspDI ATCGAT 1 cut(s) 594
BspEI TCCGGA 1 cut(s) 420
BspFNI CGCG 6 cut(s) 382, 395, 397, 468, 500, 573
BspHI TCATGA 1 cut(s) 969
BspLI GGNNCC 6 cut(s) 66, 77, 78, 183, 197, 994
BspPI GGATC 3 cut(s) 252, 451, 886
BspT107I GGYRCC 2 cut(s) 195, 992
BsrDI GCAATG 2 cut(s) 740, 894
BsrI ACTGG 3 cut(s) 102, 136, 665
BssECI CCNNGG 6 cut(s) 68, 84, 432, 453, 932, 1047
BssMI GATC 6 cut(s) 124, 244, 443, 505, 878, 1003
BssNI GRCGYC 3 cut(s) 89, 150, 356
BssT1I CCWWGG 1 cut(s) 84
Bst2UI CCWGG 3 cut(s) 70, 934, 1049
Bst4CI ACNGT 4 cut(s) 31, 64, 309, 418
BstACI GRCGYC 3 cut(s) 89, 150, 356
BstC8I GCNNGC 3 cut(s) 224, 617, 630
BstDEI CTNAG 2 cut(s) 360, 509
BstF5I GGATG 2 cut(s) 889, 1027
BstFNI CGCG 6 cut(s) 382, 395, 397, 468, 500, 573
BstHHI GCGC 5 cut(s) 382, 397, 468, 502, 573
BstKTI GATC 6 cut(s) 127, 247, 446, 508, 881, 1006
BstMAI GTCTC 3 cut(s) 32, 184, 363
BstMBI GATC 6 cut(s) 124, 244, 443, 505, 878, 1003
BstMCI CGRYCG 3 cut(s) 120, 147, 453
BstMWI GCNNNNNNNGC 9 cut(s) 116, 152, 171, 193, 232, 607, 616, 625, 686
BstNI CCWGG 3 cut(s) 70, 934, 1049
BstNSI RCATGY 2 cut(s) 619, 700
BstSCI CCNGG 4 cut(s) 68, 452, 932, 1047
BstUI CGCG 6 cut(s) 382, 395, 397, 468, 500, 573
BstV1I GCAGC 3 cut(s) 195, 644, 787
BstV2I GAAGAC 1 cut(s) 292
BstX2I RGATCY 2 cut(s) 124, 443
BstYI RGATCY 2 cut(s) 124, 443
BstZI CGGCCG 3 cut(s) 117, 144, 450
Bsu15I ATCGAT 1 cut(s) 594
BsuI GTATCC 2 cut(s) 519, 671
BsuRI GGCC 6 cut(s) 119, 146, 174, 452, 557, 689
BsuTUI ATCGAT 1 cut(s) 594
BtgZI GCGATG 2 cut(s) 383, 1006
BtsCI GGATG 2 cut(s) 889, 1027
BtsIMutI CAGTG 2 cut(s) 95, 672
Cac8I GCNNGC 3 cut(s) 224, 617, 630
CaiI CAGNNNCTG 1 cut(s) 208
CciI TCATGA 1 cut(s) 969
CfoI GCGC 5 cut(s) 382, 397, 468, 502, 573
Cfr13I GGNCC 4 cut(s) 76, 172, 418, 687
ClaI ATCGAT 1 cut(s) 594
CpoI CGGWCCG 1 cut(s) 418
CseI GACGC 5 cut(s) 78, 116, 345, 401, 488
Csp6I GTAC 3 cut(s) 496, 748, 1067
CspI CGGWCCG 1 cut(s) 418
CviAII CATG 7 cut(s) 156, 616, 697, 719, 794, 875, 970
CviQI GTAC 3 cut(s) 496, 748, 1067
DdeI CTNAG 2 cut(s) 360, 509
DpnI GATC 6 cut(s) 126, 246, 445, 507, 880, 1005
DpnII GATC 6 cut(s) 124, 244, 443, 505, 878, 1003
DrdI GACNNNNNNGTC 1 cut(s) 936
DseDI GACNNNNNNGTC 1 cut(s) 936
EaeI YGGCCR 4 cut(s) 117, 144, 450, 555
EagI CGGCCG 3 cut(s) 117, 144, 450
EciI GGCGGA 1 cut(s) 427
EclXI CGGCCG 3 cut(s) 117, 144, 450
Eco130I CCWWGG 1 cut(s) 84
Eco32I GATATC 2 cut(s) 733, 817
Eco47I GGWCC 2 cut(s) 76, 418
Eco52I CGGCCG 3 cut(s) 117, 144, 450
Eco57I CTGAAG 2 cut(s) 286, 590
Eco88I CYCGRG 1 cut(s) 176
EcoO109I RGGNCCY 1 cut(s) 76
EcoRII CCWGG 3 cut(s) 68, 932, 1047
EcoRV GATATC 2 cut(s) 733, 817
EcoT14I CCWWGG 1 cut(s) 84
ErhI CCWWGG 1 cut(s) 84
Esp3I CGTCTC 2 cut(s) 184, 363
FaeI CATG 7 cut(s) 159, 619, 700, 722, 797, 878, 973
FaqI GGGAC 2 cut(s) 89, 173
FatI CATG 7 cut(s) 155, 615, 696, 718, 793, 874, 969
FauI CCCGC 1 cut(s) 390
FauNDI CATATG 1 cut(s) 492
FbaI TGATCA 1 cut(s) 1003
FblI GTMKAC 1 cut(s) 701
Fnu4HI GCNGC 6 cut(s) 185, 209, 450, 633, 798, 801
FokI GGATG 2 cut(s) 896, 1014
Fsp4HI GCNGC 6 cut(s) 185, 209, 450, 633, 798, 801
GlaI GCGC 5 cut(s) 381, 396, 467, 501, 572
GluI GCNGC 6 cut(s) 185, 209, 450, 633, 798, 801
GsaI CCCAGC 1 cut(s) 519
GsuI CTGGAG 2 cut(s) 91, 185
HaeIII GGCC 6 cut(s) 119, 146, 174, 452, 557, 689
HapII CCGG 3 cut(s) 421, 453, 996
HgaI GACGC 5 cut(s) 78, 116, 345, 401, 488
HhaI GCGC 5 cut(s) 382, 397, 468, 502, 573
Hin1I GRCGYC 3 cut(s) 89, 150, 356
Hin1II CATG 7 cut(s) 159, 619, 700, 722, 797, 878, 973
Hin6I GCGC 5 cut(s) 380, 395, 466, 500, 571
HinP1I GCGC 5 cut(s) 380, 395, 466, 500, 571
HincII GTYRAC 3 cut(s) 133, 141, 702
HindII GTYRAC 3 cut(s) 133, 141, 702
HinfI GANTC 9 cut(s) 20, 251, 266, 272, 535, 577, 584, 638, 937
HpaII CCGG 3 cut(s) 421, 453, 996
HphI GGTGA 1 cut(s) 421
Hpy166II GTNNAC 8 cut(s) 133, 141, 351, 391, 429, 702, 750, 950
Hpy188I TCNGA 7 cut(s) 265, 271, 305, 334, 363, 510, 883
Hpy188III TCNNGA 6 cut(s) 202, 248, 421, 539, 581, 970
Hpy8I GTNNAC 8 cut(s) 133, 141, 351, 391, 429, 702, 750, 950
Hpy99I CGWCG 6 cut(s) 64, 94, 152, 155, 334, 482
HpyAV CCTTC 6 cut(s) 211, 358, 752, 782, 929, 1048
HpyCH4III ACNGT 4 cut(s) 31, 64, 309, 418
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 4 cut(s) 211, 619, 745, 887
HpyF10VI GCNNNNNNNGC 9 cut(s) 116, 152, 171, 193, 232, 607, 616, 625, 686
HpyF3I CTNAG 2 cut(s) 360, 509
HpySE526I ACGT 1 cut(s) 150
Hsp92I GRCGYC 3 cut(s) 89, 150, 356
Hsp92II CATG 7 cut(s) 159, 619, 700, 722, 797, 878, 973
HspAI GCGC 5 cut(s) 380, 395, 466, 500, 571
KflI GGGWCCC 1 cut(s) 76
Kpn2I TCCGGA 1 cut(s) 420
Ksp22I TGATCA 1 cut(s) 1003
Kzo9I GATC 6 cut(s) 124, 244, 443, 505, 878, 1003
LmnI GCTCC 4 cut(s) 56, 181, 677, 998
Lsp1109I GCAGC 3 cut(s) 195, 644, 787
LweI GCATC 1 cut(s) 874
MaeII ACGT 1 cut(s) 150
MalI GATC 6 cut(s) 126, 246, 445, 507, 880, 1005
MboI GATC 6 cut(s) 124, 244, 443, 505, 878, 1003
MboII GAAGA 4 cut(s) 292, 596, 907, 911
MflI RGATCY 2 cut(s) 124, 443
MlsI TGGCCA 1 cut(s) 557
MluCI AATT 4 cut(s) 340, 904, 942, 1041
MluNI TGGCCA 1 cut(s) 557
MlyI GAGTC 6 cut(s) 245, 260, 266, 586, 647, 946
MmeI TCCRAC 2 cut(s) 288, 312
Mox20I TGGCCA 1 cut(s) 557
MroI TCCGGA 1 cut(s) 420
MscI TGGCCA 1 cut(s) 557
MseI TTAA 3 cut(s) 278, 846, 1040
Msp20I TGGCCA 1 cut(s) 557
MspA1I CMGCKG 1 cut(s) 800
MspI CCGG 3 cut(s) 421, 453, 996
MspR9I CCNGG 4 cut(s) 70, 454, 934, 1049
MvaI CCWGG 3 cut(s) 70, 934, 1049
MvnI CGCG 6 cut(s) 382, 395, 397, 468, 500, 573
MwoI GCNNNNNNNGC 9 cut(s) 116, 152, 171, 193, 232, 607, 616, 625, 686
NciI CCSGG 1 cut(s) 454
NdeI CATATG 1 cut(s) 492
NdeII GATC 6 cut(s) 124, 244, 443, 505, 878, 1003
NlaIII CATG 7 cut(s) 159, 619, 700, 722, 797, 878, 973
NlaIV GGNNCC 6 cut(s) 66, 77, 78, 183, 197, 994
NmeAIII GCCGAG 2 cut(s) 61, 95
NspI RCATGY 2 cut(s) 619, 700
PaeI GCATGC 1 cut(s) 619
PaeR7I CTCGAG 1 cut(s) 176
PagI TCATGA 1 cut(s) 969
PciI ACATGT 1 cut(s) 696
PfeI GAWTC 3 cut(s) 20, 535, 584
PkrI GCNGC 6 cut(s) 186, 210, 451, 634, 799, 802
PleI GAGTC 6 cut(s) 245, 260, 266, 585, 646, 945
PpsI GAGTC 6 cut(s) 245, 260, 266, 585, 646, 945
PpuMI RGGWCCY 1 cut(s) 76
PscI ACATGT 1 cut(s) 696
PshBI ATTAAT 1 cut(s) 1040
Psp5II RGGWCCY 1 cut(s) 76
Psp6I CCWGG 3 cut(s) 68, 932, 1047
PspFI CCCAGC 1 cut(s) 515
PspGI CCWGG 3 cut(s) 68, 932, 1047
PspN4I GGNNCC 6 cut(s) 66, 77, 78, 183, 197, 994
PspPI GGNCC 4 cut(s) 76, 172, 418, 687
PspPPI RGGWCCY 1 cut(s) 76
PspXI VCTCGAGB 1 cut(s) 176
PstNI CAGNNNCTG 1 cut(s) 208
PsuI RGATCY 2 cut(s) 124, 443
RsaI GTAC 3 cut(s) 497, 749, 1068
RsaNI GTAC 3 cut(s) 496, 748, 1067
Rsr2I CGGWCCG 1 cut(s) 418
RsrII CGGWCCG 1 cut(s) 418
SalI GTCGAC 1 cut(s) 700
SaqAI TTAA 3 cut(s) 278, 846, 1040
SatI GCNGC 6 cut(s) 185, 209, 450, 633, 798, 801
Sau3AI GATC 6 cut(s) 124, 244, 443, 505, 878, 1003
Sau96I GGNCC 4 cut(s) 76, 172, 418, 687
SchI GAGTC 6 cut(s) 245, 260, 266, 586, 647, 946
ScrFI CCNGG 4 cut(s) 70, 454, 934, 1049
SfaNI GCATC 1 cut(s) 874
Sfr274I CTCGAG 1 cut(s) 176
SinI GGWCC 2 cut(s) 76, 418
SlaI CTCGAG 1 cut(s) 176
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
SphI GCATGC 1 cut(s) 619
Sse9I AATT 4 cut(s) 340, 904, 942, 1041
SsiI CCGC 5 cut(s) 185, 397, 412, 449, 798
StyD4I CCNGG 4 cut(s) 68, 452, 932, 1047
StyI CCWWGG 1 cut(s) 84
TaaI ACNGT 4 cut(s) 31, 64, 309, 418
TaiI ACGT 1 cut(s) 153
TaqI TCGA 6 cut(s) 23, 177, 293, 594, 701, 928
TasI AATT 4 cut(s) 340, 904, 942, 1041
TatI WGTACW 1 cut(s) 747
TauI GCSGC 3 cut(s) 187, 452, 800
TfiI GAWTC 3 cut(s) 20, 535, 584
Tru1I TTAA 3 cut(s) 278, 846, 1040
Tru9I TTAA 3 cut(s) 278, 846, 1040
TscAI CASTG 2 cut(s) 102, 672
TseI GCWGC 3 cut(s) 208, 632, 800
TspDTI ATGAA 6 cut(s) 738, 792, 912, 958, 986, 1026
TspGWI ACGGA 1 cut(s) 456
TspRI CASTG 2 cut(s) 102, 672
VpaK11BI GGWCC 2 cut(s) 76, 418
VspI ATTAAT 1 cut(s) 1040
XapI RAATTY 1 cut(s) 942
XceI RCATGY 2 cut(s) 619, 700
XhoI CTCGAG 1 cut(s) 176
XmiI GTMKAC 1 cut(s) 701
ZraI GACGTC 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.