Rh4AG294300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0014177
Physical Location & Seq
Reverse (-)
60782641 .. 60784733
2093 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG294300.1

Sequence Viewer

Length: 1062 bp
ATGGGGAAACTACTGTGTGATTCCACCACGGTCGCCGAGACATTCCAGACCTCCTCGCCCACCGTCGCCTGGCGGGACCCGCCGGCCAAGTCGACGCCGCTCCAGGAGGAGGAAGACCTGATCGAGGCCGTCGATCTCGTTGTTGACCAGTCAACGGCCGACGACGCGTGGGGCGCTGTCGTGGGCCTGGAGGAGCAGCAGCGGCGGCATCTCCAGCGGCTTCACGCCAAGGGCGTTCTCTGGAAGCATCCGGAGGCGGAGGAGGAGGACGGCAACTCCTCGGTCGTCTTCAGACTGTCGCACGGCGGAGAGGTGTCGTCCGACGGTAACTGCCTGTTCACGGCGTCGCACAAGGCGATGAAGGCGGCGCGTGAGGTCGACGCGCGGGAGTTGAGGAGGCGGACGGTGCGGAGGTTCTTGGAGGACTATGGATCTGCGAGCGCGCAGGAGAGGGACGTCATAAACGACGTCATTCGGCATCTCTACGCGCCTGATCTGAGGGCCGGGTGGGGGATACACGTCATTCAGGAGGTCAAGCTTCTGGCCAAGAAGGACGAGCGCGTGAGCCTTGACTCCGCCATTGAAGAGCTTCTTCAGCTCGGAATGCAGAGGGAGCTGGCGGCAGAGTCTATTTACAAAGAGAGATGTATCTCAGTGAGTGATGGACCGAGTTGGGCCAAATACATGTTGATCTCTGGTTCCCTGGATGATGAATATGATATCATCACGTTGCAATACACGGAGGAAGGCTTATTAACAATCGATGAAAATAGAGAAGGTCATGCTGCGGCTTTTGGAGATGATATAGCAATCGAGTGTCTTGCAACAGAGTTTAAGCGAGAGATATATGTGGTTCAAGCACATGGATCAGATGCTATGGTCGATGAGGAAAATTGTGTTTTCTTCCTTCCACACCGACCAAGGACTCGGATTTGTGAACCACCCTTCTTTCTTTTCATGAAAGGAACAGGTTGGTGTGGTGCTGGTGCTGATCACTATGAACCCCTGATTGCCCATCCCTTGTCCTTTGTTTCCCAGGAGAAGGTGGCTATGGTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

353

Amino Acids

39.53

Weight (kDa)

4.85

Isoelectric Point (pI)

52.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13100
fragaria_vesca FvH4_4g23370 FvH4_4g23370
malus_domestica MD16G1123300.v1.1
prunus_persica Prupe.1G222300_v2.0.a1 Prupe.1G222300_v2.0.a1
pyrus_communis pycom16g10550
rosa_chinensis RchiOBHm_Chr4g0429761
rosa_laevigata RLG00000007045
rosa_multiflora Rmu_sc0001935.1_g000009
rosa_roxburghii Rroxscaffold_5G00371530
rosa_rugosa Rorug04G0237900
rosa_samantha Rh4AG294300 Rh4BG300300 Rh4CG315900 Rh4DG297400
rosa_wichuraiana Rw4G025460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 459, 471
AccBSI CCGCTC 1 cut(s) 100
AccI GTMKAC 2 cut(s) 92, 378
AccII CGCG 7 cut(s) 167, 370, 383, 385, 443, 488, 561
AccIII TCCGGA 1 cut(s) 250
AclWI GGATC 2 cut(s) 439, 874
AcoI YGGCCR 3 cut(s) 84, 156, 543
AcuI CTGAAG 2 cut(s) 274, 578
AcyI GRCGYC 4 cut(s) 95, 344, 456, 468
AfiI CCNNNNNNNGG 7 cut(s) 69, 109, 124, 154, 340, 510, 1042
AflIII ACRYGT 3 cut(s) 165, 517, 684
AgsI TTSAA 2 cut(s) 584, 857
AjiI CACGTC 1 cut(s) 520
AjnI CCWGG 5 cut(s) 68, 102, 186, 702, 1035
AluBI AGCT 4 cut(s) 538, 589, 598, 616
AluI AGCT 4 cut(s) 538, 589, 598, 616
Alw26I GTCTC 1 cut(s) 32
AlwI GGATC 2 cut(s) 439, 874
Aor13HI TCCGGA 1 cut(s) 250
AoxI GGCC 7 cut(s) 84, 126, 156, 184, 501, 543, 675
ApeKI GCWGC 3 cut(s) 196, 199, 785
Asp700I GAANNNNTTC 1 cut(s) 588
AspLEI GCGC 7 cut(s) 176, 370, 385, 443, 445, 490, 561
AspS9I GGNCC 5 cut(s) 76, 184, 501, 665, 675
AsuC2I CCSGG 1 cut(s) 505
AvaII GGWCC 2 cut(s) 76, 665
BalI TGGCCA 1 cut(s) 545
BbsI GAAGAC 2 cut(s) 120, 280
BbvI GCAGC 3 cut(s) 208, 211, 772
BccI CCATC 2 cut(s) 656, 1023
BceAI ACGGC 5 cut(s) 113, 171, 286, 319, 357
BcgI CGANNNNNNTGC 2 cut(s) 803, 837
BciT130I CCWGG 5 cut(s) 70, 104, 188, 704, 1037
BciVI GTATCC 1 cut(s) 507
BclI TGATCA 1 cut(s) 991
BcnI CCSGG 1 cut(s) 505
BcoDI GTCTC 1 cut(s) 32
BfoI RGCGCY 1 cut(s) 177
BfuI GTATCC 1 cut(s) 507
Bme1390I CCNGG 6 cut(s) 70, 104, 188, 505, 704, 1037
Bme18I GGWCC 2 cut(s) 76, 665
BmgBI CACGTC 1 cut(s) 520
BmgT120I GGNCC 5 cut(s) 76, 184, 501, 665, 675
BmiI GGNNCC 3 cut(s) 77, 78, 700
BmrFI CCNGG 6 cut(s) 70, 104, 188, 505, 704, 1037
BmsI GCATC 4 cut(s) 217, 256, 487, 862
BpiI GAAGAC 2 cut(s) 120, 280
BpmI CTGGAG 3 cut(s) 86, 197, 209
BpuMI CCSGG 1 cut(s) 505
Bsa29I ATCGAT 1 cut(s) 762
BsaHI GRCGYC 4 cut(s) 95, 344, 456, 468
BsaJI CCNNGG 6 cut(s) 27, 228, 279, 702, 920, 1035
BsaWI WCCGGW 1 cut(s) 250
BsaXI ACNNNNNCTCC 2 cut(s) 260, 290
Bsc4I CCNNNNNNNGG 7 cut(s) 69, 109, 124, 154, 340, 510, 1042
Bse118I RCCGGY 1 cut(s) 82
Bse1I ACTGG 1 cut(s) 148
BseAI TCCGGA 1 cut(s) 250
BseBI CCWGG 5 cut(s) 70, 104, 188, 704, 1037
BseCI ATCGAT 1 cut(s) 762
BseDI CCNNGG 6 cut(s) 27, 228, 279, 702, 920, 1035
BseGI GGATG 3 cut(s) 247, 712, 1015
BseLI CCNNNNNNNGG 7 cut(s) 69, 109, 124, 154, 340, 510, 1042
BseMII CTCAG 2 cut(s) 488, 666
BseNI ACTGG 1 cut(s) 148
BsePI GCGCGC 1 cut(s) 441
BseRI GAGGAG 7 cut(s) 43, 122, 206, 268, 275, 278, 409
BseX3I CGGCCG 1 cut(s) 156
BseXI GCAGC 3 cut(s) 208, 211, 772
Bsh1236I CGCG 7 cut(s) 167, 370, 383, 385, 443, 488, 561
Bsh1285I CGRYCG 3 cut(s) 33, 159, 285
BshFI GGCC 7 cut(s) 86, 128, 158, 186, 503, 545, 677
BshVI ATCGAT 1 cut(s) 762
BsiEI CGRYCG 3 cut(s) 33, 159, 285
BsiSI CCGG 3 cut(s) 83, 251, 504
BslFI GGGAC 2 cut(s) 89, 467
BslI CCNNNNNNNGG 7 cut(s) 69, 109, 124, 154, 340, 510, 1042
BsmAI GTCTC 1 cut(s) 32
BsmFI GGGAC 2 cut(s) 89, 467
BsmI GAATGC 1 cut(s) 609
BsnI GGCC 7 cut(s) 86, 128, 158, 186, 503, 545, 677
Bsp13I TCCGGA 1 cut(s) 250
Bsp143I GATC 7 cut(s) 120, 133, 431, 493, 690, 866, 991
BspANI GGCC 7 cut(s) 86, 128, 158, 186, 503, 545, 677
BspCNI CTCAG 2 cut(s) 489, 665
BspDI ATCGAT 1 cut(s) 762
BspEI TCCGGA 1 cut(s) 250
BspFNI CGCG 7 cut(s) 167, 370, 383, 385, 443, 488, 561
BspHI TCATGA 1 cut(s) 957
BspLI GGNNCC 3 cut(s) 77, 78, 700
BspPI GGATC 2 cut(s) 439, 874
BspQI GCTCTTC 1 cut(s) 579
BsrBI CCGCTC 1 cut(s) 100
BsrFI RCCGGY 1 cut(s) 82
BsrI ACTGG 1 cut(s) 148
BssAI RCCGGY 1 cut(s) 82
BssECI CCNNGG 6 cut(s) 27, 228, 279, 702, 920, 1035
BssHII GCGCGC 1 cut(s) 441
BssMI GATC 7 cut(s) 120, 133, 431, 493, 690, 866, 991
BssNI GRCGYC 4 cut(s) 95, 344, 456, 468
BssT1I CCWWGG 2 cut(s) 228, 920
Bst2UI CCWGG 5 cut(s) 70, 104, 188, 704, 1037
Bst4CI ACNGT 6 cut(s) 15, 31, 64, 297, 326, 406
Bst6I CTCTTC 1 cut(s) 579
BstACI GRCGYC 4 cut(s) 95, 344, 456, 468
BstC8I GCNNGC 4 cut(s) 84, 439, 443, 618
BstDEI CTNAG 2 cut(s) 497, 652
BstDSI CCRYGG 1 cut(s) 27
BstENI CCTNNNNNAGG 1 cut(s) 122
BstF5I GGATG 3 cut(s) 247, 712, 1015
BstFNI CGCG 7 cut(s) 167, 370, 383, 385, 443, 488, 561
BstH2I RGCGCY 1 cut(s) 177
BstHHI GCGC 7 cut(s) 176, 370, 385, 443, 445, 490, 561
BstKTI GATC 7 cut(s) 123, 136, 434, 496, 693, 869, 994
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 7 cut(s) 120, 133, 431, 493, 690, 866, 991
BstMCI CGRYCG 3 cut(s) 33, 159, 285
BstNI CCWGG 5 cut(s) 70, 104, 188, 704, 1037
BstNSI RCATGY 1 cut(s) 688
BstSCI CCNGG 6 cut(s) 68, 102, 186, 503, 702, 1035
BstUI CGCG 7 cut(s) 167, 370, 383, 385, 443, 488, 561
BstV1I GCAGC 3 cut(s) 208, 211, 772
BstV2I GAAGAC 2 cut(s) 120, 280
BstX2I RGATCY 1 cut(s) 431
BstYI RGATCY 1 cut(s) 431
BstZI CGGCCG 1 cut(s) 156
Bsu15I ATCGAT 1 cut(s) 762
BsuI GTATCC 1 cut(s) 507
BsuRI GGCC 7 cut(s) 86, 128, 158, 186, 503, 545, 677
BsuTUI ATCGAT 1 cut(s) 762
BtgI CCRYGG 1 cut(s) 27
BtgZI GCGATG 1 cut(s) 371
BtrI CACGTC 1 cut(s) 520
BtsCI GGATG 3 cut(s) 247, 712, 1015
BtsIMutI CAGTG 1 cut(s) 660
Cac8I GCNNGC 4 cut(s) 84, 439, 443, 618
CciI TCATGA 1 cut(s) 957
CfoI GCGC 7 cut(s) 176, 370, 385, 443, 445, 490, 561
Cfr10I RCCGGY 1 cut(s) 82
Cfr13I GGNCC 5 cut(s) 76, 184, 501, 665, 675
ClaI ATCGAT 1 cut(s) 762
CseI GACGC 4 cut(s) 103, 173, 333, 389
CviAII CATG 4 cut(s) 685, 782, 863, 958
DdeI CTNAG 2 cut(s) 497, 652
DpnI GATC 7 cut(s) 122, 135, 433, 495, 692, 868, 993
DpnII GATC 7 cut(s) 120, 133, 431, 493, 690, 866, 991
EaeI YGGCCR 3 cut(s) 84, 156, 543
EagI CGGCCG 1 cut(s) 156
Eam1104I CTCTTC 1 cut(s) 579
EarI CTCTTC 1 cut(s) 579
EciI GGCGGA 4 cut(s) 272, 321, 415, 565
EclXI CGGCCG 1 cut(s) 156
Eco130I CCWWGG 2 cut(s) 228, 920
Eco32I GATATC 1 cut(s) 721
Eco47I GGWCC 2 cut(s) 76, 665
Eco52I CGGCCG 1 cut(s) 156
Eco57I CTGAAG 2 cut(s) 274, 578
EcoNI CCTNNNNNAGG 1 cut(s) 122
EcoO109I RGGNCCY 1 cut(s) 76
EcoRII CCWGG 5 cut(s) 68, 102, 186, 702, 1035
EcoRV GATATC 1 cut(s) 721
EcoT14I CCWWGG 2 cut(s) 228, 920
ErhI CCWWGG 2 cut(s) 228, 920
FaeI CATG 4 cut(s) 688, 785, 866, 961
FalI AAGNNNNNCTT 2 cut(s) 576, 608
FaqI GGGAC 2 cut(s) 89, 467
FatI CATG 4 cut(s) 684, 781, 862, 957
FauI CCCGC 3 cut(s) 66, 87, 378
FbaI TGATCA 1 cut(s) 991
FblI GTMKAC 2 cut(s) 92, 378
FokI GGATG 3 cut(s) 234, 719, 1002
GlaI GCGC 7 cut(s) 175, 369, 384, 442, 444, 489, 560
GsuI CTGGAG 3 cut(s) 86, 197, 209
HaeII RGCGCY 1 cut(s) 177
HaeIII GGCC 7 cut(s) 86, 128, 158, 186, 503, 545, 677
HapII CCGG 3 cut(s) 83, 251, 504
HgaI GACGC 4 cut(s) 103, 173, 333, 389
HhaI GCGC 7 cut(s) 176, 370, 385, 443, 445, 490, 561
Hin1I GRCGYC 4 cut(s) 95, 344, 456, 468
Hin1II CATG 4 cut(s) 688, 785, 866, 961
Hin6I GCGC 7 cut(s) 174, 368, 383, 441, 443, 488, 559
HinP1I GCGC 7 cut(s) 174, 368, 383, 441, 443, 488, 559
HincII GTYRAC 4 cut(s) 93, 145, 153, 379
HindII GTYRAC 4 cut(s) 93, 145, 153, 379
HindIII AAGCTT 1 cut(s) 536
HinfI GANTC 4 cut(s) 20, 572, 626, 925
HpaII CCGG 3 cut(s) 83, 251, 504
Hpy166II GTNNAC 6 cut(s) 93, 145, 153, 339, 379, 938
Hpy188I TCNGA 7 cut(s) 293, 322, 498, 602, 871, 930, 1061
Hpy188III TCNNGA 5 cut(s) 46, 241, 251, 527, 958
Hpy8I GTNNAC 6 cut(s) 93, 145, 153, 339, 379, 938
Hpy99I CGWCG 9 cut(s) 68, 97, 134, 164, 167, 326, 349, 383, 470
HpyAV CCTTC 7 cut(s) 355, 544, 740, 770, 917, 955, 1036
HpyCH4III ACNGT 6 cut(s) 15, 31, 64, 297, 326, 406
HpyCH4IV ACGT 4 cut(s) 456, 468, 519, 728
HpyCH4V TGCA 3 cut(s) 607, 733, 824
HpyF3I CTNAG 2 cut(s) 497, 652
HpySE526I ACGT 4 cut(s) 456, 468, 519, 728
Hsp92I GRCGYC 4 cut(s) 95, 344, 456, 468
Hsp92II CATG 4 cut(s) 688, 785, 866, 961
HspAI GCGC 7 cut(s) 174, 368, 383, 441, 443, 488, 559
KflI GGGWCCC 1 cut(s) 76
Kpn2I TCCGGA 1 cut(s) 250
KroI GCCGGC 1 cut(s) 82
KroNI GCCGGC 1 cut(s) 84
Ksp22I TGATCA 1 cut(s) 991
Kzo9I GATC 7 cut(s) 120, 133, 431, 493, 690, 866, 991
LguI GCTCTTC 1 cut(s) 579
LmnI GCTCC 3 cut(s) 105, 193, 613
Lsp1109I GCAGC 3 cut(s) 208, 211, 772
LweI GCATC 4 cut(s) 217, 256, 487, 862
MaeII ACGT 4 cut(s) 456, 468, 519, 728
MaeIII GTNAC 1 cut(s) 326
MalI GATC 7 cut(s) 122, 135, 433, 495, 692, 868, 993
MbiI CCGCTC 1 cut(s) 100
MboI GATC 7 cut(s) 120, 133, 431, 493, 690, 866, 991
MboII GAAGA 5 cut(s) 125, 280, 584, 596, 895
MflI RGATCY 1 cut(s) 431
MlsI TGGCCA 1 cut(s) 545
MluCI AATT 1 cut(s) 892
MluI ACGCGT 1 cut(s) 165
MluNI TGGCCA 1 cut(s) 545
MlyI GAGTC 3 cut(s) 566, 635, 919
MmeI TCCRAC 1 cut(s) 345
Mox20I TGGCCA 1 cut(s) 545
MroI TCCGGA 1 cut(s) 250
MroNI GCCGGC 1 cut(s) 82
MroXI GAANNNNTTC 1 cut(s) 588
MscI TGGCCA 1 cut(s) 545
MseI TTAA 2 cut(s) 755, 834
Msp20I TGGCCA 1 cut(s) 545
MspA1I CMGCKG 2 cut(s) 202, 217
MspI CCGG 3 cut(s) 83, 251, 504
MspR9I CCNGG 6 cut(s) 70, 104, 188, 505, 704, 1037
Mva1269I GAATGC 1 cut(s) 609
MvaI CCWGG 5 cut(s) 70, 104, 188, 704, 1037
MvnI CGCG 7 cut(s) 167, 370, 383, 385, 443, 488, 561
NaeI GCCGGC 1 cut(s) 84
NciI CCSGG 1 cut(s) 505
NdeII GATC 7 cut(s) 120, 133, 431, 493, 690, 866, 991
NgoMIV GCCGGC 1 cut(s) 82
NlaIII CATG 4 cut(s) 688, 785, 866, 961
NlaIV GGNNCC 3 cut(s) 77, 78, 700
NmeAIII GCCGAG 1 cut(s) 61
NspI RCATGY 1 cut(s) 688
PagI TCATGA 1 cut(s) 957
PauI GCGCGC 1 cut(s) 441
PciI ACATGT 1 cut(s) 684
PciSI GCTCTTC 1 cut(s) 579
PcsI WCGNNNNNNNCGW 4 cut(s) 129, 231, 353, 462
PctI GAATGC 1 cut(s) 609
PdiI GCCGGC 1 cut(s) 84
PdmI GAANNNNTTC 1 cut(s) 588
PfeI GAWTC 1 cut(s) 20
PfoI TCCNGGA 1 cut(s) 102
PleI GAGTC 3 cut(s) 566, 634, 919
PpsI GAGTC 3 cut(s) 566, 634, 919
PpuMI RGGWCCY 1 cut(s) 76
PscI ACATGT 1 cut(s) 684
Psp5II RGGWCCY 1 cut(s) 76
Psp6I CCWGG 5 cut(s) 68, 102, 186, 702, 1035
PspGI CCWGG 5 cut(s) 68, 102, 186, 702, 1035
PspN4I GGNNCC 3 cut(s) 77, 78, 700
PspPI GGNCC 5 cut(s) 76, 184, 501, 665, 675
PspPPI RGGWCCY 1 cut(s) 76
PsuI RGATCY 1 cut(s) 431
PteI GCGCGC 1 cut(s) 441
SalI GTCGAC 2 cut(s) 91, 377
SapI GCTCTTC 1 cut(s) 579
SaqAI TTAA 2 cut(s) 755, 834
Sau3AI GATC 7 cut(s) 120, 133, 431, 493, 690, 866, 991
Sau96I GGNCC 5 cut(s) 76, 184, 501, 665, 675
SchI GAGTC 3 cut(s) 566, 635, 919
ScrFI CCNGG 6 cut(s) 70, 104, 188, 505, 704, 1037
SfaNI GCATC 4 cut(s) 217, 256, 487, 862
SinI GGWCC 2 cut(s) 76, 665
Sse9I AATT 1 cut(s) 892
StyD4I CCNGG 6 cut(s) 68, 102, 186, 503, 702, 1035
StyI CCWWGG 2 cut(s) 228, 920
TaaI ACNGT 6 cut(s) 15, 31, 64, 297, 326, 406
TaiI ACGT 4 cut(s) 459, 471, 522, 731
TaqI TCGA 7 cut(s) 92, 123, 132, 378, 762, 813, 882
TaqII GACCGA 2 cut(s) 271, 682
TasI AATT 1 cut(s) 892
TauI GCSGC 7 cut(s) 100, 205, 208, 220, 368, 623, 791
TfiI GAWTC 1 cut(s) 20
Tru1I TTAA 2 cut(s) 755, 834
Tru9I TTAA 2 cut(s) 755, 834
TscAI CASTG 1 cut(s) 660
TseI GCWGC 3 cut(s) 196, 199, 785
TspDTI ATGAA 6 cut(s) 374, 726, 780, 946, 974, 1014
TspGWI ACGGA 1 cut(s) 755
TspRI CASTG 1 cut(s) 660
VpaK11BI GGWCC 2 cut(s) 76, 665
XagI CCTNNNNNAGG 1 cut(s) 122
XceI RCATGY 1 cut(s) 688
XmiI GTMKAC 2 cut(s) 92, 378
XmnI GAANNNNTTC 1 cut(s) 588
ZraI GACGTC 2 cut(s) 457, 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.