Rroxscaffold_5G00371530

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0014177
Physical Location & Seq
Reverse (-)
53108796 .. 53111445
2650 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00371530.1

Sequence Viewer

Length: 858 bp
ATGGGGAAACTACTGTGTGATTCCACCACGGTTGCCGAGACATTCCAGACCTCCTCGCCCACCGTCGCCTGGCGGGACCCGCCGGCCAAGTCGGCGCCGCTCCAGGAGGAGGAAGACCTGATCGAGGCCGTCGATCTCGTTGTTGACCAGTCAACGGCCGACGACGCGTGGGGCGCTGTCGTGGGCCTGGAGGAGCAGCAGCGGCGGCATCTCCAGCGGCTTCACGCCAAGGGCGTTCTCTGGAAGCATCCAGAGGCGGAGGACGGCGACTCCTCGGTCGTCTTCAGACTGTCGCACGGCGGAGAGGTGTCGTCCGACGGTAACTGCCTGTTCACGGCGTCGCACAAGGCGATGAAGGCGGCGCGTGAGGTCGACGCGCGGGAGTTGAGGAGGCGGACGGTGCGGAGGTTCTTGGAGGACTATGGATCTGCGAGCGCGGGGGAGAGGGACGTCATAAACGACGTCATTCGGCATCTCTACGCGCCTGATCTGAGGGCCGGGTGGGGGATACACGTCATTCAGGAGGTCAAGCTTCTGGCCAAGAAGGACGAGCGCGTGAGTCTTGACTCCGCCATTGACGAGCTTCTTCAGCTCGGAATGCAGAGGGAGCTGGCGGCAGAGTCTATTTACAAAGAGAGATGTATCTCAGTTCAAGCACATGGATCAGATGCTATGGTCGATGAGGAAAATTGTGTTTTCTTCCTTCCACACCGACCAAGGACTCGGATTTGTGAACCACCCTTCTTTCTTTTCATGAAAGGAACAGGTTGGTGTGGTGCTGGTGCTGATCACTATGAACCCCTGATTGCCCATCCCTTGTCCTTTGTTTCCCAGGAGAAGGTGGCTATGGTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

285

Amino Acids

31.77

Weight (kDa)

5.33

Isoelectric Point (pI)

47.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13100
fragaria_vesca FvH4_4g23370 FvH4_4g23370
malus_domestica MD16G1123300.v1.1
prunus_persica Prupe.1G222300_v2.0.a1 Prupe.1G222300_v2.0.a1
pyrus_communis pycom16g10550
rosa_chinensis RchiOBHm_Chr4g0429761
rosa_laevigata RLG00000007045
rosa_multiflora Rmu_sc0001935.1_g000009
rosa_roxburghii Rroxscaffold_5G00371530
rosa_rugosa Rorug04G0237900
rosa_samantha Rh4AG294300 Rh4BG300300 Rh4CG315900 Rh4DG297400
rosa_wichuraiana Rw4G025460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 275
AatII GACGTC 2 cut(s) 453, 465
AccB1I GGYRCC 1 cut(s) 94
AccBSI CCGCTC 1 cut(s) 100
AccI GTMKAC 1 cut(s) 372
AccII CGCG 7 cut(s) 167, 364, 377, 379, 437, 482, 555
AclWI GGATC 2 cut(s) 433, 670
AcoI YGGCCR 3 cut(s) 84, 156, 537
AcuI CTGAAG 2 cut(s) 268, 572
AcyI GRCGYC 4 cut(s) 95, 338, 450, 462
AfiI CCNNNNNNNGG 7 cut(s) 69, 109, 124, 154, 334, 504, 838
AflIII ACRYGT 2 cut(s) 165, 511
AgsI TTSAA 1 cut(s) 653
AjiI CACGTC 1 cut(s) 514
AjnI CCWGG 4 cut(s) 68, 102, 186, 831
AluBI AGCT 4 cut(s) 532, 583, 592, 610
AluI AGCT 4 cut(s) 532, 583, 592, 610
Alw26I GTCTC 1 cut(s) 32
AlwI GGATC 2 cut(s) 433, 670
AoxI GGCC 6 cut(s) 84, 126, 156, 184, 495, 537
ApeKI GCWGC 2 cut(s) 196, 199
AspLEI GCGC 7 cut(s) 97, 176, 364, 379, 437, 484, 555
AspS9I GGNCC 3 cut(s) 76, 184, 495
AsuC2I CCSGG 1 cut(s) 499
AvaII GGWCC 1 cut(s) 76
BalI TGGCCA 1 cut(s) 539
BanI GGYRCC 1 cut(s) 94
BbsI GAAGAC 2 cut(s) 120, 274
BbvI GCAGC 2 cut(s) 208, 211
BccI CCATC 1 cut(s) 819
BceAI ACGGC 5 cut(s) 113, 171, 280, 313, 351
BciT130I CCWGG 4 cut(s) 70, 104, 188, 833
BciVI GTATCC 1 cut(s) 501
BclI TGATCA 1 cut(s) 787
BcnI CCSGG 1 cut(s) 499
BcoDI GTCTC 1 cut(s) 32
BfoI RGCGCY 2 cut(s) 98, 177
BfuI GTATCC 1 cut(s) 501
BglI GCCNNNNNGGC 1 cut(s) 92
BisI GCNGC 8 cut(s) 98, 197, 200, 203, 206, 218, 360, 615
BlsI GCNGC 8 cut(s) 99, 198, 201, 204, 207, 219, 361, 616
Bme1390I CCNGG 5 cut(s) 70, 104, 188, 499, 833
Bme18I GGWCC 1 cut(s) 76
BmgBI CACGTC 1 cut(s) 514
BmgT120I GGNCC 3 cut(s) 76, 184, 495
BmiI GGNNCC 3 cut(s) 77, 78, 96
BmrFI CCNGG 5 cut(s) 70, 104, 188, 499, 833
BmsI GCATC 4 cut(s) 217, 256, 481, 658
BpiI GAAGAC 2 cut(s) 120, 274
BpmI CTGGAG 3 cut(s) 86, 197, 209
BpuMI CCSGG 1 cut(s) 499
BsaHI GRCGYC 4 cut(s) 95, 338, 450, 462
BsaJI CCNNGG 5 cut(s) 27, 228, 273, 716, 831
BsaXI ACNNNNNCTCC 2 cut(s) 254, 284
Bsc4I CCNNNNNNNGG 7 cut(s) 69, 109, 124, 154, 334, 504, 838
Bse118I RCCGGY 1 cut(s) 82
Bse1I ACTGG 1 cut(s) 148
BseBI CCWGG 4 cut(s) 70, 104, 188, 833
BseDI CCNNGG 5 cut(s) 27, 228, 273, 716, 831
BseGI GGATG 2 cut(s) 247, 811
BseLI CCNNNNNNNGG 7 cut(s) 69, 109, 124, 154, 334, 504, 838
BseMII CTCAG 2 cut(s) 482, 660
BseNI ACTGG 1 cut(s) 148
BseRI GAGGAG 5 cut(s) 43, 122, 206, 262, 403
BseX3I CGGCCG 1 cut(s) 156
BseXI GCAGC 2 cut(s) 208, 211
Bsh1236I CGCG 7 cut(s) 167, 364, 377, 379, 437, 482, 555
Bsh1285I CGRYCG 2 cut(s) 159, 279
BshFI GGCC 6 cut(s) 86, 128, 158, 186, 497, 539
BshNI GGYRCC 1 cut(s) 94
BsiEI CGRYCG 2 cut(s) 159, 279
BsiSI CCGG 2 cut(s) 83, 498
BslFI GGGAC 2 cut(s) 89, 461
BslI CCNNNNNNNGG 7 cut(s) 69, 109, 124, 154, 334, 504, 838
BsmAI GTCTC 1 cut(s) 32
BsmFI GGGAC 2 cut(s) 89, 461
BsmI GAATGC 1 cut(s) 603
BsnI GGCC 6 cut(s) 86, 128, 158, 186, 497, 539
Bsp143I GATC 6 cut(s) 120, 133, 425, 487, 662, 787
BspANI GGCC 6 cut(s) 86, 128, 158, 186, 497, 539
BspCNI CTCAG 2 cut(s) 483, 659
BspFNI CGCG 7 cut(s) 167, 364, 377, 379, 437, 482, 555
BspHI TCATGA 1 cut(s) 753
BspLI GGNNCC 3 cut(s) 77, 78, 96
BspPI GGATC 2 cut(s) 433, 670
BspT107I GGYRCC 1 cut(s) 94
BsrBI CCGCTC 1 cut(s) 100
BsrFI RCCGGY 1 cut(s) 82
BsrI ACTGG 1 cut(s) 148
BssAI RCCGGY 1 cut(s) 82
BssECI CCNNGG 5 cut(s) 27, 228, 273, 716, 831
BssMI GATC 6 cut(s) 120, 133, 425, 487, 662, 787
BssNI GRCGYC 4 cut(s) 95, 338, 450, 462
BssT1I CCWWGG 2 cut(s) 228, 716
Bst2UI CCWGG 4 cut(s) 70, 104, 188, 833
Bst4CI ACNGT 6 cut(s) 15, 31, 64, 291, 320, 400
BstACI GRCGYC 4 cut(s) 95, 338, 450, 462
BstC8I GCNNGC 3 cut(s) 84, 433, 612
BstDEI CTNAG 2 cut(s) 491, 646
BstDSI CCRYGG 1 cut(s) 27
BstENI CCTNNNNNAGG 1 cut(s) 122
BstF5I GGATG 2 cut(s) 247, 811
BstFNI CGCG 7 cut(s) 167, 364, 377, 379, 437, 482, 555
BstH2I RGCGCY 2 cut(s) 98, 177
BstHHI GCGC 7 cut(s) 97, 176, 364, 379, 437, 484, 555
BstKTI GATC 6 cut(s) 123, 136, 428, 490, 665, 790
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 6 cut(s) 120, 133, 425, 487, 662, 787
BstMCI CGRYCG 2 cut(s) 159, 279
BstNI CCWGG 4 cut(s) 70, 104, 188, 833
BstSCI CCNGG 5 cut(s) 68, 102, 186, 497, 831
BstUI CGCG 7 cut(s) 167, 364, 377, 379, 437, 482, 555
BstV1I GCAGC 2 cut(s) 208, 211
BstV2I GAAGAC 2 cut(s) 120, 274
BstX2I RGATCY 1 cut(s) 425
BstYI RGATCY 1 cut(s) 425
BstZI CGGCCG 1 cut(s) 156
BsuI GTATCC 1 cut(s) 501
BsuRI GGCC 6 cut(s) 86, 128, 158, 186, 497, 539
BtgI CCRYGG 1 cut(s) 27
BtgZI GCGATG 1 cut(s) 365
BtrI CACGTC 1 cut(s) 514
BtsCI GGATG 2 cut(s) 247, 811
Cac8I GCNNGC 3 cut(s) 84, 433, 612
CciI TCATGA 1 cut(s) 753
CfoI GCGC 7 cut(s) 97, 176, 364, 379, 437, 484, 555
Cfr10I RCCGGY 1 cut(s) 82
Cfr13I GGNCC 3 cut(s) 76, 184, 495
CseI GACGC 3 cut(s) 173, 327, 383
CspCI CAANNNNNGTGG 2 cut(s) 13, 48
CviAII CATG 2 cut(s) 659, 754
DdeI CTNAG 2 cut(s) 491, 646
DinI GGCGCC 1 cut(s) 96
DpnI GATC 6 cut(s) 122, 135, 427, 489, 664, 789
DpnII GATC 6 cut(s) 120, 133, 425, 487, 662, 787
DrdI GACNNNNNNGTC 1 cut(s) 275
DseDI GACNNNNNNGTC 1 cut(s) 275
EaeI YGGCCR 3 cut(s) 84, 156, 537
EagI CGGCCG 1 cut(s) 156
EciI GGCGGA 4 cut(s) 272, 315, 409, 559
EclXI CGGCCG 1 cut(s) 156
Eco130I CCWWGG 2 cut(s) 228, 716
Eco47I GGWCC 1 cut(s) 76
Eco52I CGGCCG 1 cut(s) 156
Eco57I CTGAAG 2 cut(s) 268, 572
EcoNI CCTNNNNNAGG 1 cut(s) 122
EcoO109I RGGNCCY 1 cut(s) 76
EcoRII CCWGG 4 cut(s) 68, 102, 186, 831
EcoT14I CCWWGG 2 cut(s) 228, 716
EgeI GGCGCC 1 cut(s) 96
EheI GGCGCC 1 cut(s) 96
ErhI CCWWGG 2 cut(s) 228, 716
FaeI CATG 2 cut(s) 662, 757
FaiI YATR 7 cut(s) 423, 455, 660, 674, 755, 795, 848
FaqI GGGAC 2 cut(s) 89, 461
FatI CATG 2 cut(s) 658, 753
FauI CCCGC 4 cut(s) 66, 87, 372, 430
FbaI TGATCA 1 cut(s) 787
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 8 cut(s) 98, 197, 200, 203, 206, 218, 360, 615
FokI GGATG 2 cut(s) 234, 798
Fsp4HI GCNGC 8 cut(s) 98, 197, 200, 203, 206, 218, 360, 615
GlaI GCGC 7 cut(s) 96, 175, 363, 378, 436, 483, 554
GluI GCNGC 8 cut(s) 98, 197, 200, 203, 206, 218, 360, 615
GsuI CTGGAG 3 cut(s) 86, 197, 209
HaeII RGCGCY 2 cut(s) 98, 177
HaeIII GGCC 6 cut(s) 86, 128, 158, 186, 497, 539
HapII CCGG 2 cut(s) 83, 498
HgaI GACGC 3 cut(s) 173, 327, 383
HhaI GCGC 7 cut(s) 97, 176, 364, 379, 437, 484, 555
Hin1I GRCGYC 4 cut(s) 95, 338, 450, 462
Hin1II CATG 2 cut(s) 662, 757
Hin6I GCGC 7 cut(s) 95, 174, 362, 377, 435, 482, 553
HinP1I GCGC 7 cut(s) 95, 174, 362, 377, 435, 482, 553
HincII GTYRAC 3 cut(s) 145, 153, 373
HindII GTYRAC 3 cut(s) 145, 153, 373
HindIII AAGCTT 1 cut(s) 530
HinfI GANTC 6 cut(s) 20, 269, 559, 566, 620, 721
HpaII CCGG 2 cut(s) 83, 498
Hpy166II GTNNAC 5 cut(s) 145, 153, 333, 373, 734
Hpy188I TCNGA 7 cut(s) 287, 316, 492, 596, 667, 726, 857
Hpy188III TCNNGA 6 cut(s) 46, 241, 251, 521, 563, 754
Hpy8I GTNNAC 5 cut(s) 145, 153, 333, 373, 734
Hpy99I CGWCG 8 cut(s) 68, 134, 164, 167, 320, 343, 377, 464
HpyAV CCTTC 5 cut(s) 349, 538, 713, 751, 832
HpyCH4III ACNGT 6 cut(s) 15, 31, 64, 291, 320, 400
HpyCH4IV ACGT 3 cut(s) 450, 462, 513
HpyCH4V TGCA 1 cut(s) 601
HpyF3I CTNAG 2 cut(s) 491, 646
HpySE526I ACGT 3 cut(s) 450, 462, 513
Hsp92I GRCGYC 4 cut(s) 95, 338, 450, 462
Hsp92II CATG 2 cut(s) 662, 757
HspAI GCGC 7 cut(s) 95, 174, 362, 377, 435, 482, 553
KasI GGCGCC 1 cut(s) 94
KflI GGGWCCC 1 cut(s) 76
KroI GCCGGC 1 cut(s) 82
KroNI GCCGGC 1 cut(s) 84
Ksp22I TGATCA 1 cut(s) 787
Kzo9I GATC 6 cut(s) 120, 133, 425, 487, 662, 787
LmnI GCTCC 3 cut(s) 105, 193, 607
Lsp1109I GCAGC 2 cut(s) 208, 211
LweI GCATC 4 cut(s) 217, 256, 481, 658
MaeII ACGT 3 cut(s) 450, 462, 513
MaeIII GTNAC 1 cut(s) 320
MalI GATC 6 cut(s) 122, 135, 427, 489, 664, 789
MbiI CCGCTC 1 cut(s) 100
MboI GATC 6 cut(s) 120, 133, 425, 487, 662, 787
MboII GAAGA 4 cut(s) 125, 274, 578, 691
MflI RGATCY 1 cut(s) 425
MlsI TGGCCA 1 cut(s) 539
MluCI AATT 1 cut(s) 688
MluI ACGCGT 1 cut(s) 165
MluNI TGGCCA 1 cut(s) 539
Mly113I GGCGCC 1 cut(s) 95
MlyI GAGTC 5 cut(s) 263, 560, 568, 629, 715
MmeI TCCRAC 1 cut(s) 339
Mox20I TGGCCA 1 cut(s) 539
MroNI GCCGGC 1 cut(s) 82
MscI TGGCCA 1 cut(s) 539
Msp20I TGGCCA 1 cut(s) 539
MspA1I CMGCKG 2 cut(s) 202, 217
MspI CCGG 2 cut(s) 83, 498
MspR9I CCNGG 5 cut(s) 70, 104, 188, 499, 833
Mva1269I GAATGC 1 cut(s) 603
MvaI CCWGG 4 cut(s) 70, 104, 188, 833
MvnI CGCG 7 cut(s) 167, 364, 377, 379, 437, 482, 555
NaeI GCCGGC 1 cut(s) 84
NarI GGCGCC 1 cut(s) 95
NciI CCSGG 1 cut(s) 499
NdeII GATC 6 cut(s) 120, 133, 425, 487, 662, 787
NgoMIV GCCGGC 1 cut(s) 82
NlaIII CATG 2 cut(s) 662, 757
NlaIV GGNNCC 3 cut(s) 77, 78, 96
NmeAIII GCCGAG 1 cut(s) 61
PagI TCATGA 1 cut(s) 753
PcsI WCGNNNNNNNCGW 4 cut(s) 129, 231, 347, 456
PctI GAATGC 1 cut(s) 603
PdiI GCCGGC 1 cut(s) 84
PfeI GAWTC 1 cut(s) 20
PfoI TCCNGGA 1 cut(s) 102
PkrI GCNGC 8 cut(s) 99, 198, 201, 204, 207, 219, 361, 616
PleI GAGTC 5 cut(s) 263, 560, 567, 628, 715
PluTI GGCGCC 1 cut(s) 98
PpsI GAGTC 5 cut(s) 263, 560, 567, 628, 715
PpuMI RGGWCCY 1 cut(s) 76
Psp5II RGGWCCY 1 cut(s) 76
Psp6I CCWGG 4 cut(s) 68, 102, 186, 831
PspGI CCWGG 4 cut(s) 68, 102, 186, 831
PspN4I GGNNCC 3 cut(s) 77, 78, 96
PspPI GGNCC 3 cut(s) 76, 184, 495
PspPPI RGGWCCY 1 cut(s) 76
PsuI RGATCY 1 cut(s) 425
SalI GTCGAC 1 cut(s) 371
SatI GCNGC 8 cut(s) 98, 197, 200, 203, 206, 218, 360, 615
Sau3AI GATC 6 cut(s) 120, 133, 425, 487, 662, 787
Sau96I GGNCC 3 cut(s) 76, 184, 495
SchI GAGTC 5 cut(s) 263, 560, 568, 629, 715
ScrFI CCNGG 5 cut(s) 70, 104, 188, 499, 833
SfaNI GCATC 4 cut(s) 217, 256, 481, 658
SfoI GGCGCC 1 cut(s) 96
SinI GGWCC 1 cut(s) 76
Sse9I AATT 1 cut(s) 688
SspDI GGCGCC 1 cut(s) 94
StyD4I CCNGG 5 cut(s) 68, 102, 186, 497, 831
StyI CCWWGG 2 cut(s) 228, 716
TaaI ACNGT 6 cut(s) 15, 31, 64, 291, 320, 400
TaiI ACGT 3 cut(s) 453, 465, 516
TaqI TCGA 4 cut(s) 123, 132, 372, 678
TaqII GACCGA 1 cut(s) 265
TasI AATT 1 cut(s) 688
TauI GCSGC 6 cut(s) 100, 205, 208, 220, 362, 617
TfiI GAWTC 1 cut(s) 20
TseI GCWGC 2 cut(s) 196, 199
TspDTI ATGAA 4 cut(s) 368, 742, 770, 810
VpaK11BI GGWCC 1 cut(s) 76
XagI CCTNNNNNAGG 1 cut(s) 122
XmiI GTMKAC 1 cut(s) 372
ZraI GACGTC 2 cut(s) 451, 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.