FvH4_4g23520

Squamosa promoter-binding-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0008130
Physical Location & Seq
Forward (+)
25983654 .. 25986122
2469 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g23520.t4

Sequence Viewer

Length: 813 bp
ATGAACAACGATTCAAAGGGCAAGTTGAAGGGCCCTGCTGCCGCTACGATCAACACATCTTCAAAGAGCCGCCGACAAACCAATGCTATAATCCAAGTTGCTTCATGCCTGGTTGATGGTTGCAAATCCGACCTTAGTCTGTGCAGGGAGTATCACCAGCGCCATAAAGTATGTGAGCTCCATTCGAAGACCCCAAAGGTCATGATTCAGGGGCAGGAGAAGCGGTTCTGTCAGCAGTGCAGCAGGTTCCATTCATTGGATGAGTTTGATGAAGGAAAAAGAAGTTGTAGAAGGCGTCTTGCCGGACACAACAAACGCCGAAGGAAGCCTCAGTTAGAACCTATGCCCAAAAGTTTGGGAGCATTTCTTTCTTATTACCAAGGCACAGTTTTCACACCATTCAGTACTCCACAAATGTACCCACCACCTGTTGTAAAGAACTCTTCTTGGCATGAGGGTCCTATTGGCTCCATGATCTCTGGAGCTTCTTCTGTTTGCCAACCGCTTCTTGATACCAACTTTGCATTGGGAAATTATGGCAACAACCACAAAATGTTCTCCAATGAATTCTTCAGTGGCAACATTGAACCAAGTTGTGCTCCGTCTCTTCTGTCATTGACACCAGCAGTCCCGGAGGAGACTGGTTTGAGCCTCACAGGACAGGCCAGTTCAATCTGCTCGGTTCAGTCCTTTGTCCCTAGACTTCACCACTACAATGATTTGGGAATGCAGAATAAAGCAGCAATTCCAAACCTTGTTACTGATGGAAGCAACAATTCCAATCTCCTTTGCATTCAGCAGATGTTCCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SBP

Protein Analysis

271

Amino Acids

29.88

Weight (kDa)

9.22

Isoelectric Point (pI)

58.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 36 - 109 1.5e-31 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 234
AccB7I CCANNNNNTGG 1 cut(s) 256
AciI CCGC 4 cut(s) 42, 70, 223, 503
AcsI RAATTY 1 cut(s) 566
AcuI CTGAAG 1 cut(s) 556
AcyI GRCGYC 1 cut(s) 295
AfaI GTAC 2 cut(s) 406, 419
AfiI CCNNNNNNNGG 1 cut(s) 256
AgsI TTSAA 5 cut(s) 15, 28, 63, 587, 672
AjnI CCWGG 1 cut(s) 108
AjuI GAANNNNNNNTTGG 2 cut(s) 554, 586
AloI GAACNNNNNNTCC 2 cut(s) 209, 241
AluBI AGCT 2 cut(s) 178, 485
AluI AGCT 2 cut(s) 178, 485
Alw21I GWGCWC 2 cut(s) 180, 601
Alw26I GTCTC 2 cut(s) 609, 632
AoxI GGCC 2 cut(s) 31, 663
ApaI GGGCCC 1 cut(s) 35
ApeKI GCWGC 3 cut(s) 38, 240, 740
ApoI RAATTY 1 cut(s) 566
Asp700I GAANNNNTTC 1 cut(s) 224
AspLEI GCGC 1 cut(s) 162
AspS9I GGNCC 3 cut(s) 31, 32, 458
AsuC2I CCSGG 1 cut(s) 632
AsuHPI GGTGA 2 cut(s) 146, 698
AsuII TTCGAA 1 cut(s) 185
AvaII GGWCC 1 cut(s) 458
BaeGI GKGCMC 1 cut(s) 35
BaeI ACNNNNGTAYC 2 cut(s) 401, 434
BanII GRGCYC 2 cut(s) 35, 180
BbsI GAAGAC 1 cut(s) 194
Bbv12I GWGCWC 2 cut(s) 180, 601
BbvI GCAGC 3 cut(s) 25, 252, 752
BccI CCATC 2 cut(s) 110, 758
BciT130I CCWGG 1 cut(s) 110
BcnI CCSGG 1 cut(s) 632
BcoDI GTCTC 2 cut(s) 609, 632
BfaI CTAG 2 cut(s) 699, 811
BfoI RGCGCY 1 cut(s) 163
BfuAI ACCTGC 1 cut(s) 234
BisI GCNGC 5 cut(s) 39, 42, 70, 241, 741
BlsI GCNGC 5 cut(s) 40, 43, 71, 242, 742
BmcAI AGTACT 1 cut(s) 406
Bme1390I CCNGG 2 cut(s) 110, 632
Bme18I GGWCC 1 cut(s) 458
BmgT120I GGNCC 3 cut(s) 31, 32, 458
BmiI GGNNCC 4 cut(s) 33, 248, 459, 469
BmrFI CCNGG 2 cut(s) 110, 632
BoxI GACNNNNGTC 1 cut(s) 135
BpiI GAAGAC 1 cut(s) 194
BpmI CTGGAG 1 cut(s) 501
Bpu14I TTCGAA 1 cut(s) 185
BpuMI CCSGG 1 cut(s) 632
BsaHI GRCGYC 1 cut(s) 295
BsaJI CCNNGG 1 cut(s) 379
BsaXI ACNNNNNCTCC 2 cut(s) 209, 239
Bsc4I CCNNNNNNNGG 1 cut(s) 256
Bse1I ACTGG 2 cut(s) 646, 666
BseBI CCWGG 1 cut(s) 110
BseDI CCNNGG 1 cut(s) 379
BseGI GGATG 1 cut(s) 265
BseLI CCNNNNNNNGG 1 cut(s) 256
BseMII CTCAG 1 cut(s) 344
BseNI ACTGG 2 cut(s) 646, 666
BseRI GAGGAG 1 cut(s) 650
BseSI GKGCMC 1 cut(s) 35
BseXI GCAGC 3 cut(s) 25, 252, 752
BsgI GTGCAG 2 cut(s) 163, 259
BshFI GGCC 2 cut(s) 33, 665
BsiHKAI GWGCWC 2 cut(s) 180, 601
BsiSI CCGG 2 cut(s) 303, 632
BslFI GGGAC 2 cut(s) 614, 680
BslI CCNNNNNNNGG 1 cut(s) 256
BsmAI GTCTC 2 cut(s) 609, 632
BsmBI CGTCTC 1 cut(s) 609
BsmFI GGGAC 2 cut(s) 614, 680
BsmI GAATGC 2 cut(s) 732, 792
BsnI GGCC 2 cut(s) 33, 665
Bsp119I TTCGAA 1 cut(s) 185
Bsp120I GGGCCC 1 cut(s) 31
Bsp1286I GDGCHC 3 cut(s) 35, 180, 601
Bsp143I GATC 2 cut(s) 48, 474
BspACI CCGC 4 cut(s) 42, 70, 223, 503
BspANI GGCC 2 cut(s) 33, 665
BspCNI CTCAG 1 cut(s) 343
BspHI TCATGA 1 cut(s) 201
BspLI GGNNCC 4 cut(s) 33, 248, 459, 469
BspMI ACCTGC 1 cut(s) 234
BspT104I TTCGAA 1 cut(s) 185
BsrI ACTGG 2 cut(s) 646, 666
BssECI CCNNGG 1 cut(s) 379
BssMI GATC 2 cut(s) 48, 474
BssNI GRCGYC 1 cut(s) 295
BssT1I CCWWGG 1 cut(s) 379
Bst2UI CCWGG 1 cut(s) 110
Bst4CI ACNGT 1 cut(s) 388
Bst6I CTCTTC 2 cut(s) 448, 612
BstACI GRCGYC 1 cut(s) 295
BstBI TTCGAA 1 cut(s) 185
BstDEI CTNAG 2 cut(s) 134, 330
BstF5I GGATG 1 cut(s) 265
BstH2I RGCGCY 1 cut(s) 163
BstHHI GCGC 1 cut(s) 162
BstKTI GATC 2 cut(s) 51, 477
BstMAI GTCTC 2 cut(s) 609, 632
BstMBI GATC 2 cut(s) 48, 474
BstMWI GCNNNNNNNGC 1 cut(s) 220
BstNI CCWGG 1 cut(s) 110
BstPAI GACNNNNGTC 1 cut(s) 135
BstSCI CCNGG 2 cut(s) 108, 630
BstSLI GKGCMC 1 cut(s) 35
BstV1I GCAGC 3 cut(s) 25, 252, 752
BstV2I GAAGAC 1 cut(s) 194
BstXI CCANNNNNNTGG 1 cut(s) 355
BsuRI GGCC 2 cut(s) 33, 665
BtsCI GGATG 1 cut(s) 265
BtsI GCAGTG 1 cut(s) 242
BtsIMutI CAGTG 2 cut(s) 242, 580
BveI ACCTGC 1 cut(s) 234
CciI TCATGA 1 cut(s) 201
CfoI GCGC 1 cut(s) 162
Cfr13I GGNCC 3 cut(s) 31, 32, 458
CseI GACGC 1 cut(s) 284
Csp6I GTAC 2 cut(s) 405, 418
CviAII CATG 4 cut(s) 105, 202, 452, 472
CviJI RGCY 8 cut(s) 33, 69, 178, 328, 468, 485, 651, 665
CviKI_1 RGCY 8 cut(s) 33, 69, 178, 328, 468, 485, 651, 665
CviQI GTAC 2 cut(s) 405, 418
DdeI CTNAG 2 cut(s) 134, 330
DpnI GATC 2 cut(s) 50, 476
DpnII GATC 2 cut(s) 48, 474
Eam1104I CTCTTC 2 cut(s) 448, 612
EarI CTCTTC 2 cut(s) 448, 612
Ecl136II GAGCTC 1 cut(s) 178
Eco130I CCWWGG 1 cut(s) 379
Eco24I GRGCYC 2 cut(s) 35, 180
Eco47I GGWCC 1 cut(s) 458
Eco53kI GAGCTC 1 cut(s) 178
Eco57I CTGAAG 1 cut(s) 556
EcoICRI GAGCTC 1 cut(s) 178
EcoO109I RGGNCCY 3 cut(s) 31, 32, 458
EcoRI GAATTC 1 cut(s) 566
EcoRII CCWGG 1 cut(s) 108
EcoT14I CCWWGG 1 cut(s) 379
EcoT38I GRGCYC 2 cut(s) 35, 180
ErhI CCWWGG 1 cut(s) 379
Esp3I CGTCTC 1 cut(s) 609
FaeI CATG 4 cut(s) 108, 205, 455, 475
FaiI YATR 9 cut(s) 89, 106, 165, 172, 203, 344, 453, 473, 537
FaqI GGGAC 2 cut(s) 614, 680
FatI CATG 4 cut(s) 104, 201, 451, 471
Fnu4HI GCNGC 5 cut(s) 39, 42, 70, 241, 741
FokI GGATG 1 cut(s) 272
FriOI GRGCYC 2 cut(s) 35, 180
Fsp4HI GCNGC 5 cut(s) 39, 42, 70, 241, 741
FspBI CTAG 2 cut(s) 699, 811
GlaI GCGC 1 cut(s) 161
GluI GCNGC 5 cut(s) 39, 42, 70, 241, 741
GsuI CTGGAG 1 cut(s) 501
HaeII RGCGCY 1 cut(s) 163
HaeIII GGCC 2 cut(s) 33, 665
HapII CCGG 2 cut(s) 303, 632
HgaI GACGC 1 cut(s) 284
HhaI GCGC 1 cut(s) 162
Hin1I GRCGYC 1 cut(s) 295
Hin1II CATG 4 cut(s) 108, 205, 455, 475
Hin6I GCGC 1 cut(s) 160
HinP1I GCGC 1 cut(s) 160
HinfI GANTC 2 cut(s) 11, 205
HpaII CCGG 2 cut(s) 303, 632
HphI GGTGA 2 cut(s) 146, 698
Hpy188I TCNGA 1 cut(s) 130
Hpy188III TCNNGA 3 cut(s) 202, 480, 509
HpyAV CCTTC 4 cut(s) 22, 266, 285, 315
HpyCH4III ACNGT 1 cut(s) 388
HpyCH4V TGCA 6 cut(s) 123, 144, 240, 524, 730, 792
HpyF10VI GCNNNNNNNGC 1 cut(s) 220
HpyF3I CTNAG 2 cut(s) 134, 330
Hsp92I GRCGYC 1 cut(s) 295
Hsp92II CATG 4 cut(s) 108, 205, 455, 475
HspAI GCGC 1 cut(s) 160
Kzo9I GATC 2 cut(s) 48, 474
LmnI GCTCC 5 cut(s) 183, 359, 473, 482, 604
Lsp1109I GCAGC 3 cut(s) 25, 252, 752
MaeI CTAG 2 cut(s) 699, 811
MaeIII GTNAC 1 cut(s) 757
MalI GATC 2 cut(s) 50, 476
MboI GATC 2 cut(s) 48, 474
MboII GAAGA 6 cut(s) 51, 199, 435, 480, 562, 599
MhlI GDGCHC 3 cut(s) 35, 180, 601
MluCI AATT 4 cut(s) 532, 566, 744, 775
MmeI TCCRAC 1 cut(s) 153
MnlI CCTC 4 cut(s) 339, 448, 628, 662
MroXI GAANNNNTTC 1 cut(s) 224
MslI CAYNNNNRTG 1 cut(s) 714
MspI CCGG 2 cut(s) 303, 632
MspR9I CCNGG 2 cut(s) 110, 632
Mva1269I GAATGC 2 cut(s) 732, 792
MvaI CCWGG 1 cut(s) 110
MwoI GCNNNNNNNGC 1 cut(s) 220
NciI CCSGG 1 cut(s) 632
NdeII GATC 2 cut(s) 48, 474
NlaIII CATG 4 cut(s) 108, 205, 455, 475
NlaIV GGNNCC 4 cut(s) 33, 248, 459, 469
NspV TTCGAA 1 cut(s) 185
PagI TCATGA 1 cut(s) 201
PctI GAATGC 2 cut(s) 732, 792
PdmI GAANNNNTTC 1 cut(s) 224
PfeI GAWTC 2 cut(s) 11, 205
PflMI CCANNNNNTGG 1 cut(s) 256
PfoI TCCNGGA 1 cut(s) 630
PkrI GCNGC 5 cut(s) 40, 43, 71, 242, 742
PpuMI RGGWCCY 1 cut(s) 458
PshAI GACNNNNGTC 1 cut(s) 135
Psp124BI GAGCTC 1 cut(s) 180
Psp5II RGGWCCY 1 cut(s) 458
Psp6I CCWGG 1 cut(s) 108
PspGI CCWGG 1 cut(s) 108
PspN4I GGNNCC 4 cut(s) 33, 248, 459, 469
PspOMI GGGCCC 1 cut(s) 31
PspPI GGNCC 3 cut(s) 31, 32, 458
PspPPI RGGWCCY 1 cut(s) 458
RsaI GTAC 2 cut(s) 406, 419
RsaNI GTAC 2 cut(s) 405, 418
RseI CAYNNNNRTG 1 cut(s) 714
SacI GAGCTC 1 cut(s) 180
SatI GCNGC 5 cut(s) 39, 42, 70, 241, 741
Sau3AI GATC 2 cut(s) 48, 474
Sau96I GGNCC 3 cut(s) 31, 32, 458
ScaI AGTACT 1 cut(s) 406
ScrFI CCNGG 2 cut(s) 110, 632
SduI GDGCHC 3 cut(s) 35, 180, 601
SetI ASST 8 cut(s) 135, 180, 201, 248, 343, 430, 487, 756
SfuI TTCGAA 1 cut(s) 185
SinI GGWCC 1 cut(s) 458
SmiMI CAYNNNNRTG 1 cut(s) 714
Sse9I AATT 4 cut(s) 532, 566, 744, 775
SsiI CCGC 4 cut(s) 42, 70, 223, 503
SspMI CTAG 2 cut(s) 699, 811
SstI GAGCTC 1 cut(s) 180
StyD4I CCNGG 2 cut(s) 108, 630
StyI CCWWGG 1 cut(s) 379
TaaI ACNGT 1 cut(s) 388
TaqI TCGA 1 cut(s) 185
TasI AATT 4 cut(s) 532, 566, 744, 775
TatI WGTACW 1 cut(s) 404
TauI GCSGC 2 cut(s) 44, 72
TfiI GAWTC 2 cut(s) 11, 205
TscAI CASTG 2 cut(s) 242, 580
TseI GCWGC 3 cut(s) 38, 240, 740
TspDTI ATGAA 5 cut(s) 17, 93, 243, 285, 579
TspGWI ACGGA 1 cut(s) 591
TspRI CASTG 2 cut(s) 242, 580
Van91I CCANNNNNTGG 1 cut(s) 256
VpaK11BI GGWCC 1 cut(s) 458
XapI RAATTY 1 cut(s) 566
XcmI CCANNNNNNNNNTGG 1 cut(s) 523
XmnI GAANNNNTTC 1 cut(s) 224
XspI CTAG 2 cut(s) 699, 811
ZrmI AGTACT 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.