MD16G1122000.v1.1

Squamosa promoter-binding-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0008130
Physical Location & Seq
Reverse (-)
8792038 .. 8794876
2839 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1122000.v1.1.491

Sequence Viewer

Length: 930 bp
ATGGAAGATGGATCAAAGGGCAAGTTGAAGGATCCTGGGATCTCTATGAGGAAGCCATCTTCGAGTACTACATCTTTGAAAAGACCACGACAACCCAATAACGAAACCAAGATTGCTGCATGCTTGGTTGATGGGTGCAATTCGGACCTTAGCAAATGCCGGGATTATCATCGACGGCATAAGGTATGTGAGCTCCATTCAAAGACCTCAAAAGTTACAATCAAGGGCCAGGAACGGCGGTTCTGTCAGCAGTGCAGCAGGTTCCATTCGTTGGTGGAATTCGATGAAGGAAAAAGAAGCTGCAGAAGACGTCTTGATGGACACAACAAACGTAGGAGGAAGCCTCAGTCAGAATCCATGCCCACAAATTTGGGAAGATTTCTTTCCAATTACCAAGGCACAAGATTCTTATCATTCGGTAATCCACAAATGTGTGCAGCAAAGAACACACATTTGTGGGGCTGCGCATTGAAACCGGAGAATGATTGTGTGCTGCTTGATAACCACTCACAATTAAATGAGACAAGAAGAAGCTCATATGCTTACCGAGAAAAACAAATCCCATTTATGCAAGGCTTTGAATACGCGCACTCAGAAGCTGCTGTCTACCAACCATTTGCAAATACCAACTTTGCACCGGGAAATTCTAGCAACAGCCATAGAATGTTTGCCAATGGATTATATGGCGACATAGAATCTAATCGTGCTCTCTCTCTTCTGTCATGGACACGAGCAACACAAGAGGAGATTGGGCTGAGCAACACAGCACAGTCCAGTTCAGTCTGCCCGGCTCAGTCCATGATCCCAAGAATCCACTACAATAATCTGGGAGTGCAGGATGAGGCTGCAATTTCTAATTTGGTTAGTGATGGCAACAGCATTGGCAATCTCCACTGCCAAGGCAAGTTTCAGAATTCAGATTATTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SBP

Protein Analysis

310

Amino Acids

34.83

Weight (kDa)

9.33

Isoelectric Point (pI)

53.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 41 - 114 1.8e-30 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 313
Acc16I TGCGCA 1 cut(s) 466
Acc36I ACCTGC 1 cut(s) 249
AccB7I CCANNNNNTGG 1 cut(s) 271
AccI GTMKAC 1 cut(s) 606
AccII CGCG 1 cut(s) 587
AciI CCGC 1 cut(s) 238
AclWI GGATC 5 cut(s) 19, 26, 39, 47, 796
AcsI RAATTY 4 cut(s) 278, 367, 643, 913
AcyI GRCGYC 1 cut(s) 310
AfaI GTAC 1 cut(s) 67
AfiI CCNNNNNNNGG 1 cut(s) 271
AgsI TTSAA 5 cut(s) 28, 79, 201, 472, 581
AjnI CCWGG 2 cut(s) 34, 228
AjuI GAANNNNNNNTTGG 2 cut(s) 891, 923
AleI CACNNNNGTG 2 cut(s) 430, 454
AloI GAACNNNNNNTCC 2 cut(s) 224, 256
AluBI AGCT 4 cut(s) 193, 300, 534, 599
AluI AGCT 4 cut(s) 193, 300, 534, 599
Alw21I GWGCWC 2 cut(s) 195, 709
Alw26I GTCTC 1 cut(s) 515
AlwI GGATC 5 cut(s) 19, 26, 39, 47, 796
AlwNI CAGNNNCTG 1 cut(s) 599
AoxI GGCC 1 cut(s) 226
ApeKI GCWGC 8 cut(s) 116, 255, 300, 437, 462, 493, 599, 845
ApoI RAATTY 4 cut(s) 278, 367, 643, 913
AspLEI GCGC 2 cut(s) 467, 589
AspS9I GGNCC 2 cut(s) 145, 226
AsuC2I CCSGG 3 cut(s) 161, 639, 788
AvaII GGWCC 1 cut(s) 145
BamHI GGATCC 1 cut(s) 31
BanII GRGCYC 1 cut(s) 195
BauI CACGAG 1 cut(s) 729
BbsI GAAGAC 1 cut(s) 313
Bbv12I GWGCWC 2 cut(s) 195, 709
BbvI GCAGC 8 cut(s) 103, 267, 287, 449, 449, 480, 586, 832
BccI CCATC 5 cut(s) 2, 64, 125, 311, 863
BceAI ACGGC 2 cut(s) 191, 251
BciT130I CCWGG 2 cut(s) 36, 230
BcnI CCSGG 3 cut(s) 161, 639, 788
BcoDI GTCTC 1 cut(s) 515
BfaI CTAG 1 cut(s) 648
BfmI CTRYAG 1 cut(s) 301
BfuAI ACCTGC 1 cut(s) 249
BisI GCNGC 8 cut(s) 117, 256, 301, 438, 463, 494, 600, 846
BlpI GCTNAGC 1 cut(s) 755
BlsI GCNGC 8 cut(s) 118, 257, 302, 439, 464, 495, 601, 847
BmcAI AGTACT 1 cut(s) 67
Bme1390I CCNGG 5 cut(s) 36, 161, 230, 639, 788
Bme18I GGWCC 1 cut(s) 145
BmgT120I GGNCC 2 cut(s) 145, 226
BmiI GGNNCC 2 cut(s) 33, 263
BmrFI CCNGG 5 cut(s) 36, 161, 230, 639, 788
BpiI GAAGAC 1 cut(s) 313
BplI GAGNNNNNCTC 2 cut(s) 328, 360
Bpu10I CCTNAGC 1 cut(s) 149
Bpu1102I GCTNAGC 1 cut(s) 755
BpuMI CCSGG 3 cut(s) 161, 639, 788
BsaBI GATNNNNATC 2 cut(s) 168, 409
BsaHI GRCGYC 1 cut(s) 310
BsaJI CCNNGG 3 cut(s) 35, 394, 898
BsaWI WCCGGW 1 cut(s) 475
Bsc4I CCNNNNNNNGG 1 cut(s) 271
Bse1I ACTGG 1 cut(s) 774
Bse8I GATNNNNATC 2 cut(s) 168, 409
BseBI CCWGG 2 cut(s) 36, 230
BseDI CCNNGG 3 cut(s) 35, 394, 898
BseGI GGATG 1 cut(s) 844
BseJI GATNNNNATC 2 cut(s) 168, 409
BseLI CCNNNNNNNGG 1 cut(s) 271
BseMII CTCAG 4 cut(s) 359, 606, 746, 806
BseNI ACTGG 1 cut(s) 774
BseRI GAGGAG 1 cut(s) 758
BseXI GCAGC 8 cut(s) 103, 267, 287, 449, 449, 480, 586, 832
BsgI GTGCAG 3 cut(s) 274, 456, 854
Bsh1236I CGCG 1 cut(s) 587
BshFI GGCC 1 cut(s) 228
BsiHKAI GWGCWC 2 cut(s) 195, 709
BsiSI CCGG 4 cut(s) 160, 476, 638, 788
BslI CCNNNNNNNGG 1 cut(s) 271
BsmAI GTCTC 1 cut(s) 515
BsnI GGCC 1 cut(s) 228
Bsp1286I GDGCHC 2 cut(s) 195, 709
Bsp143I GATC 4 cut(s) 11, 31, 39, 801
Bsp1720I GCTNAGC 1 cut(s) 755
BspACI CCGC 1 cut(s) 238
BspANI GGCC 1 cut(s) 228
BspCNI CTCAG 4 cut(s) 358, 605, 747, 805
BspFNI CGCG 1 cut(s) 587
BspLI GGNNCC 2 cut(s) 33, 263
BspMAI CTGCAG 1 cut(s) 305
BspMI ACCTGC 1 cut(s) 249
BspPI GGATC 5 cut(s) 19, 26, 39, 47, 796
BsrI ACTGG 1 cut(s) 774
BssECI CCNNGG 3 cut(s) 35, 394, 898
BssMI GATC 4 cut(s) 11, 31, 39, 801
BssNI GRCGYC 1 cut(s) 310
BssSI CACGAG 1 cut(s) 729
BssT1I CCWWGG 2 cut(s) 394, 898
Bst2BI CACGAG 1 cut(s) 729
Bst2UI CCWGG 2 cut(s) 36, 230
Bst4CI ACNGT 1 cut(s) 771
Bst6I CTCTTC 1 cut(s) 720
BstACI GRCGYC 1 cut(s) 310
BstC8I GCNNGC 1 cut(s) 121
BstDEI CTNAG 5 cut(s) 149, 345, 592, 755, 792
BstF5I GGATG 1 cut(s) 844
BstFNI CGCG 1 cut(s) 587
BstHHI GCGC 2 cut(s) 467, 589
BstKTI GATC 4 cut(s) 14, 34, 42, 804
BstMAI GTCTC 1 cut(s) 515
BstMBI GATC 4 cut(s) 11, 31, 39, 801
BstNI CCWGG 2 cut(s) 36, 230
BstNSI RCATGY 1 cut(s) 123
BstSCI CCNGG 5 cut(s) 34, 159, 228, 637, 786
BstSFI CTRYAG 1 cut(s) 301
BstUI CGCG 1 cut(s) 587
BstV1I GCAGC 8 cut(s) 103, 267, 287, 449, 449, 480, 586, 832
BstV2I GAAGAC 1 cut(s) 313
BstX2I RGATCY 2 cut(s) 31, 39
BstXI CCANNNNNNTGG 1 cut(s) 370
BstYI RGATCY 2 cut(s) 31, 39
BsuRI GGCC 1 cut(s) 228
BtsCI GGATG 1 cut(s) 844
BtsI GCAGTG 2 cut(s) 257, 892
BtsIMutI CAGTG 2 cut(s) 257, 892
BveI ACCTGC 1 cut(s) 249
Cac8I GCNNGC 1 cut(s) 121
CaiI CAGNNNCTG 1 cut(s) 599
CfoI GCGC 2 cut(s) 467, 589
Cfr13I GGNCC 2 cut(s) 145, 226
Csp6I GTAC 1 cut(s) 66
CviAII CATG 4 cut(s) 120, 358, 723, 799
CviQI GTAC 1 cut(s) 66
DdeI CTNAG 5 cut(s) 149, 345, 592, 755, 792
DpnI GATC 4 cut(s) 13, 33, 41, 803
DpnII GATC 4 cut(s) 11, 31, 39, 801
Eam1104I CTCTTC 1 cut(s) 720
EarI CTCTTC 1 cut(s) 720
Ecl136II GAGCTC 1 cut(s) 193
Eco130I CCWWGG 2 cut(s) 394, 898
Eco24I GRGCYC 1 cut(s) 195
Eco47I GGWCC 1 cut(s) 145
Eco53kI GAGCTC 1 cut(s) 193
EcoICRI GAGCTC 1 cut(s) 193
EcoRI GAATTC 2 cut(s) 278, 913
EcoRII CCWGG 2 cut(s) 34, 228
EcoT14I CCWWGG 2 cut(s) 394, 898
EcoT38I GRGCYC 1 cut(s) 195
ErhI CCWWGG 2 cut(s) 394, 898
FaeI CATG 4 cut(s) 123, 361, 726, 802
FatI CATG 4 cut(s) 119, 357, 722, 798
FauNDI CATATG 1 cut(s) 538
FblI GTMKAC 1 cut(s) 606
Fnu4HI GCNGC 8 cut(s) 117, 256, 301, 438, 463, 494, 600, 846
FokI GGATG 1 cut(s) 851
FriOI GRGCYC 1 cut(s) 195
Fsp4HI GCNGC 8 cut(s) 117, 256, 301, 438, 463, 494, 600, 846
FspBI CTAG 1 cut(s) 648
FspI TGCGCA 1 cut(s) 466
GlaI GCGC 2 cut(s) 466, 588
GluI GCNGC 8 cut(s) 117, 256, 301, 438, 463, 494, 600, 846
HaeIII GGCC 1 cut(s) 228
HapII CCGG 4 cut(s) 160, 476, 638, 788
HhaI GCGC 2 cut(s) 467, 589
Hin1I GRCGYC 1 cut(s) 310
Hin1II CATG 4 cut(s) 123, 361, 726, 802
Hin6I GCGC 2 cut(s) 465, 587
HinP1I GCGC 2 cut(s) 465, 587
HinfI GANTC 4 cut(s) 353, 405, 695, 810
HpaII CCGG 4 cut(s) 160, 476, 638, 788
Hpy166II GTNNAC 1 cut(s) 607
Hpy188I TCNGA 5 cut(s) 145, 352, 595, 912, 919
Hpy188III TCNNGA 2 cut(s) 314, 927
Hpy8I GTNNAC 1 cut(s) 607
Hpy99I CGWCG 1 cut(s) 177
HpyAV CCTTC 2 cut(s) 22, 281
HpyCH4III ACNGT 1 cut(s) 771
HpyCH4IV ACGT 2 cut(s) 310, 331
HpyF3I CTNAG 5 cut(s) 149, 345, 592, 755, 792
HpySE526I ACGT 2 cut(s) 310, 331
Hsp92I GRCGYC 1 cut(s) 310
Hsp92II CATG 4 cut(s) 123, 361, 726, 802
HspAI GCGC 2 cut(s) 465, 587
Kzo9I GATC 4 cut(s) 11, 31, 39, 801
LmnI GCTCC 1 cut(s) 198
Lsp1109I GCAGC 8 cut(s) 103, 267, 287, 449, 449, 480, 586, 832
MaeI CTAG 1 cut(s) 648
MaeII ACGT 2 cut(s) 310, 331
MaeIII GTNAC 1 cut(s) 214
MalI GATC 4 cut(s) 13, 33, 41, 803
MboI GATC 4 cut(s) 11, 31, 39, 801
MboII GAAGA 6 cut(s) 17, 51, 318, 387, 540, 707
MflI RGATCY 2 cut(s) 31, 39
MhlI GDGCHC 2 cut(s) 195, 709
MluCI AATT 9 cut(s) 139, 278, 367, 388, 512, 643, 849, 856, 913
MnlI CCTC 6 cut(s) 42, 217, 330, 354, 736, 835
MseI TTAA 1 cut(s) 515
MslI CAYNNNNRTG 2 cut(s) 430, 454
MspI CCGG 4 cut(s) 160, 476, 638, 788
MspR9I CCNGG 5 cut(s) 36, 161, 230, 639, 788
MvaI CCWGG 2 cut(s) 36, 230
MvnI CGCG 1 cut(s) 587
NciI CCSGG 3 cut(s) 161, 639, 788
NdeI CATATG 1 cut(s) 538
NdeII GATC 4 cut(s) 11, 31, 39, 801
NlaIII CATG 4 cut(s) 123, 361, 726, 802
NlaIV GGNNCC 2 cut(s) 33, 263
NsbI TGCGCA 1 cut(s) 466
NspI RCATGY 1 cut(s) 123
OliI CACNNNNGTG 2 cut(s) 430, 454
PaeI GCATGC 1 cut(s) 123
PfeI GAWTC 4 cut(s) 353, 405, 695, 810
PflMI CCANNNNNTGG 1 cut(s) 271
PkrI GCNGC 8 cut(s) 118, 257, 302, 439, 464, 495, 601, 847
Psp124BI GAGCTC 1 cut(s) 195
Psp6I CCWGG 2 cut(s) 34, 228
PspGI CCWGG 2 cut(s) 34, 228
PspN4I GGNNCC 2 cut(s) 33, 263
PspPI GGNCC 2 cut(s) 145, 226
PstI CTGCAG 1 cut(s) 305
PstNI CAGNNNCTG 1 cut(s) 599
PsuI RGATCY 2 cut(s) 31, 39
RsaI GTAC 1 cut(s) 67
RsaNI GTAC 1 cut(s) 66
RseI CAYNNNNRTG 2 cut(s) 430, 454
SacI GAGCTC 1 cut(s) 195
SaqAI TTAA 1 cut(s) 515
SatI GCNGC 8 cut(s) 117, 256, 301, 438, 463, 494, 600, 846
Sau3AI GATC 4 cut(s) 11, 31, 39, 801
Sau96I GGNCC 2 cut(s) 145, 226
ScaI AGTACT 1 cut(s) 67
ScrFI CCNGG 5 cut(s) 36, 161, 230, 639, 788
SduI GDGCHC 2 cut(s) 195, 709
SfcI CTRYAG 1 cut(s) 301
SinI GGWCC 1 cut(s) 145
SmiMI CAYNNNNRTG 2 cut(s) 430, 454
SphI GCATGC 1 cut(s) 123
Sse9I AATT 9 cut(s) 139, 278, 367, 388, 512, 643, 849, 856, 913
SsiI CCGC 1 cut(s) 238
SspMI CTAG 1 cut(s) 648
SstI GAGCTC 1 cut(s) 195
StyD4I CCNGG 5 cut(s) 34, 159, 228, 637, 786
StyI CCWWGG 2 cut(s) 394, 898
TaaI ACNGT 1 cut(s) 771
TaiI ACGT 2 cut(s) 313, 334
TaqI TCGA 3 cut(s) 62, 172, 282
TasI AATT 9 cut(s) 139, 278, 367, 388, 512, 643, 849, 856, 913
TatI WGTACW 1 cut(s) 65
TfiI GAWTC 4 cut(s) 353, 405, 695, 810
Tru1I TTAA 1 cut(s) 515
Tru9I TTAA 1 cut(s) 515
TscAI CASTG 2 cut(s) 257, 899
TseI GCWGC 8 cut(s) 116, 255, 300, 437, 462, 493, 599, 845
TspDTI ATGAA 1 cut(s) 300
TspRI CASTG 2 cut(s) 257, 899
Van91I CCANNNNNTGG 1 cut(s) 271
VpaK11BI GGWCC 1 cut(s) 145
XapI RAATTY 4 cut(s) 278, 367, 643, 913
XceI RCATGY 1 cut(s) 123
XmiI GTMKAC 1 cut(s) 606
XspI CTAG 1 cut(s) 648
ZraI GACGTC 1 cut(s) 311
ZrmI AGTACT 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.