Rh4DG299300

Squamosa promoter-binding-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0008130
Physical Location & Seq
Forward (+)
52135990 .. 52138374
2385 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG299300.1

Sequence Viewer

Length: 993 bp
ATGGGTGCAATTCCGACCTTAGCATGTGCAGGGAGTATCACCAGCGCCACAAAGTATGTGAGCTCCATTCAAAGACCCCAAAGGTCACGATTCAGGGACAGGAAAAGCGATTCTGTCAGCAGTGCAGCAGGTAAGAGTGAAAAGGATAGAAAAGTTATTAGCAATTTCTTCATTTTGAAGTGTGTACATAATTACATATGCATGAAATCTAATCAGCAGCATTTGCGATTCTTTAAAATCCCTAATCCATTGGACATACATCAGTTAATGTTTTTCTTTCCACATCACCCTGGTTTCTTTCCTTGCTCAAGTCTATTAACATCTAATCTTACATTACCAAGGTTCCATTCATTGGAAGAGTTTGATGAAGGAAAAAGAAGCTGCAGAAGGCGTCTTGCTGGACACAACAAACGCCGAAGGAAGCCTCAGTTGGAACCTATGCCCACAAGTTTAGGAGCATTTCTTTCTGATTACCGAGGCGCAACATTTTCAGCATTCAGTACTCCACAACTCTATCCAACAAATGCTGTAATGAACTCTGCTTGGGAAGATTTTCCAAATCACAGCCACTCCCAATTAAGTGTGTCAAGAAGAAACTCATTTTCAGAATCTTTACCATGCTATAGCCACAGAGTAGGCATTGGCTTCATGCACTCTGAAGCTGCTTCTTCTGTTTGCCAACCACTTCTTGGTACCAACTTTGCATTGGGAAATTATGGCAACAACCACAAAATTTTCTCCAATGAGTTCTTCAGTGGCAACGTTGAATCAAGTTGCGCTCGCTCTCTTCTGTCATTGACACCAGCAGTCCCGGAGGAGACTGGTTTGAGCCTCATAGGACAGACAAGTTCAATCTGCTCAGCTCAGTCCTTCGTTCCTGTACTTCACCACTACAATGATTTGGGAATGCAGGGTGGAGCAGTAGTTTCTAATCTAGTTACTGATGGAAGCAACAATTCCAATCTCCTTTGCATTCAGCAGATGTTTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SBP

Protein Analysis

330

Amino Acids

36.65

Weight (kDa)

9.31

Isoelectric Point (pI)

51.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 114 - 141 5.6e-09 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 119
Acc65I GGTACC 1 cut(s) 692
AccB1I GGYRCC 1 cut(s) 692
AccB7I CCANNNNNTGG 2 cut(s) 352, 689
AclI AACGTT 1 cut(s) 762
AcsI RAATTY 1 cut(s) 732
AcuI CTGAAG 2 cut(s) 678, 736
AcyI GRCGYC 1 cut(s) 391
AfaI GTAC 4 cut(s) 186, 502, 694, 882
AfiI CCNNNNNNNGG 2 cut(s) 352, 689
AgsI TTSAA 4 cut(s) 71, 178, 767, 852
AjnI CCWGG 1 cut(s) 289
AjuI GAANNNNNNNTTGG 6 cut(s) 331, 363, 413, 445, 734, 766
AluBI AGCT 4 cut(s) 63, 381, 662, 863
AluI AGCT 4 cut(s) 63, 381, 662, 863
Alw21I GWGCWC 1 cut(s) 65
Alw26I GTCTC 1 cut(s) 812
ApeKI GCWGC 4 cut(s) 125, 217, 381, 662
ApoI RAATTY 1 cut(s) 732
Asp700I GAANNNNTTC 1 cut(s) 552
Asp718I GGTACC 1 cut(s) 692
AspLEI GCGC 3 cut(s) 47, 482, 779
AsuC2I CCSGG 1 cut(s) 812
AsuHPI GGTGA 3 cut(s) 31, 278, 878
BanI GGYRCC 1 cut(s) 692
BanII GRGCYC 1 cut(s) 65
Bbv12I GWGCWC 1 cut(s) 65
BbvI GCAGC 4 cut(s) 137, 229, 368, 649
BccI CCATC 1 cut(s) 938
BciT130I CCWGG 1 cut(s) 291
BcnI CCSGG 1 cut(s) 812
BcoDI GTCTC 1 cut(s) 812
BfaI CTAG 2 cut(s) 935, 991
BfmI CTRYAG 2 cut(s) 382, 622
BfoI RGCGCY 1 cut(s) 48
BfuAI ACCTGC 1 cut(s) 119
BisI GCNGC 4 cut(s) 126, 218, 382, 663
BlpI GCTNAGC 1 cut(s) 859
BlsI GCNGC 4 cut(s) 127, 219, 383, 664
BmcAI AGTACT 1 cut(s) 502
Bme1390I CCNGG 2 cut(s) 291, 812
BmiI GGNNCC 3 cut(s) 344, 435, 694
BmrFI CCNGG 2 cut(s) 291, 812
Bpu10I CCTNAGC 1 cut(s) 19
Bpu1102I GCTNAGC 1 cut(s) 859
BpuEI CTTGAG 1 cut(s) 292
BpuMI CCSGG 1 cut(s) 812
BsaHI GRCGYC 1 cut(s) 391
BsaJI CCNNGG 3 cut(s) 289, 338, 475
BsaXI ACNNNNNCTCC 4 cut(s) 554, 584, 909, 939
Bsc4I CCNNNNNNNGG 2 cut(s) 352, 689
Bse1I ACTGG 1 cut(s) 826
BseBI CCWGG 1 cut(s) 291
BseDI CCNNGG 3 cut(s) 289, 338, 475
BseLI CCNNNNNNNGG 2 cut(s) 352, 689
BseMII CTCAG 3 cut(s) 440, 873, 878
BseNI ACTGG 1 cut(s) 826
BseRI GAGGAG 1 cut(s) 830
BseXI GCAGC 4 cut(s) 137, 229, 368, 649
BsgI GTGCAG 2 cut(s) 48, 144
BshNI GGYRCC 1 cut(s) 692
BsiHKAI GWGCWC 1 cut(s) 65
BsiSI CCGG 1 cut(s) 812
BslFI GGGAC 2 cut(s) 110, 794
BslI CCNNNNNNNGG 2 cut(s) 352, 689
BsmAI GTCTC 1 cut(s) 812
BsmFI GGGAC 2 cut(s) 110, 794
BsmI GAATGC 3 cut(s) 494, 912, 972
Bsp1286I GDGCHC 1 cut(s) 65
Bsp1407I TGTACA 1 cut(s) 184
Bsp1720I GCTNAGC 1 cut(s) 859
BspCNI CTCAG 3 cut(s) 439, 872, 877
BspLI GGNNCC 3 cut(s) 344, 435, 694
BspMAI CTGCAG 1 cut(s) 386
BspMI ACCTGC 1 cut(s) 119
BspT107I GGYRCC 1 cut(s) 692
BsrGI TGTACA 1 cut(s) 184
BsrI ACTGG 1 cut(s) 826
BssECI CCNNGG 3 cut(s) 289, 338, 475
BssNI GRCGYC 1 cut(s) 391
BssT1I CCWWGG 1 cut(s) 338
Bst2UI CCWGG 1 cut(s) 291
Bst6I CTCTTC 2 cut(s) 351, 792
BstACI GRCGYC 1 cut(s) 391
BstAPI GCANNNNNTGC 1 cut(s) 223
BstAUI TGTACA 1 cut(s) 184
BstC8I GCNNGC 1 cut(s) 781
BstDEI CTNAG 4 cut(s) 19, 426, 859, 864
BstH2I RGCGCY 1 cut(s) 48
BstHHI GCGC 3 cut(s) 47, 482, 779
BstMAI GTCTC 1 cut(s) 812
BstMWI GCNNNNNNNGC 1 cut(s) 223
BstNI CCWGG 1 cut(s) 291
BstNSI RCATGY 1 cut(s) 27
BstSCI CCNGG 2 cut(s) 289, 810
BstSFI CTRYAG 2 cut(s) 382, 622
BstV1I GCAGC 4 cut(s) 137, 229, 368, 649
BtsI GCAGTG 1 cut(s) 127
BtsIMutI CAGTG 2 cut(s) 127, 760
BveI ACCTGC 1 cut(s) 119
Cac8I GCNNGC 1 cut(s) 781
CfoI GCGC 3 cut(s) 47, 482, 779
CseI GACGC 1 cut(s) 380
Csp6I GTAC 4 cut(s) 185, 501, 693, 881
CviAII CATG 4 cut(s) 24, 202, 618, 649
CviJI RGCY 9 cut(s) 63, 381, 424, 567, 627, 645, 662, 831, 863
CviKI_1 RGCY 9 cut(s) 63, 381, 424, 567, 627, 645, 662, 831, 863
CviQI GTAC 4 cut(s) 185, 501, 693, 881
DdeI CTNAG 4 cut(s) 19, 426, 859, 864
DraI TTTAAA 1 cut(s) 235
Eam1104I CTCTTC 2 cut(s) 351, 792
EarI CTCTTC 2 cut(s) 351, 792
Ecl136II GAGCTC 1 cut(s) 63
Eco130I CCWWGG 1 cut(s) 338
Eco24I GRGCYC 1 cut(s) 65
Eco53kI GAGCTC 1 cut(s) 63
Eco57I CTGAAG 2 cut(s) 678, 736
EcoICRI GAGCTC 1 cut(s) 63
EcoRII CCWGG 1 cut(s) 289
EcoT14I CCWWGG 1 cut(s) 338
EcoT22I ATGCAT 1 cut(s) 203
EcoT38I GRGCYC 1 cut(s) 65
ErhI CCWWGG 1 cut(s) 338
FaeI CATG 4 cut(s) 27, 205, 621, 652
FaqI GGGAC 2 cut(s) 110, 794
FatI CATG 4 cut(s) 23, 201, 617, 648
FauNDI CATATG 1 cut(s) 197
Fnu4HI GCNGC 4 cut(s) 126, 218, 382, 663
FriOI GRGCYC 1 cut(s) 65
Fsp4HI GCNGC 4 cut(s) 126, 218, 382, 663
FspBI CTAG 2 cut(s) 935, 991
GlaI GCGC 3 cut(s) 46, 481, 778
GluI GCNGC 4 cut(s) 126, 218, 382, 663
HaeII RGCGCY 1 cut(s) 48
HapII CCGG 1 cut(s) 812
HgaI GACGC 1 cut(s) 380
HhaI GCGC 3 cut(s) 47, 482, 779
Hin1I GRCGYC 1 cut(s) 391
Hin1II CATG 4 cut(s) 27, 205, 621, 652
Hin6I GCGC 3 cut(s) 45, 480, 777
HinP1I GCGC 3 cut(s) 45, 480, 777
HinfI GANTC 5 cut(s) 90, 110, 228, 608, 767
HpaII CCGG 1 cut(s) 812
HphI GGTGA 3 cut(s) 31, 278, 878
Hpy166II GTNNAC 1 cut(s) 185
Hpy188I TCNGA 4 cut(s) 15, 469, 607, 658
Hpy188III TCNNGA 2 cut(s) 87, 588
Hpy8I GTNNAC 1 cut(s) 185
HpyAV CCTTC 4 cut(s) 362, 381, 411, 880
HpyCH4IV ACGT 1 cut(s) 762
HpyCH4V TGCA 9 cut(s) 8, 29, 125, 201, 384, 652, 704, 910, 972
HpyF10VI GCNNNNNNNGC 1 cut(s) 223
HpyF3I CTNAG 4 cut(s) 19, 426, 859, 864
HpySE526I ACGT 1 cut(s) 762
Hsp92I GRCGYC 1 cut(s) 391
Hsp92II CATG 4 cut(s) 27, 205, 621, 652
HspAI GCGC 3 cut(s) 45, 480, 777
KpnI GGTACC 1 cut(s) 696
LmnI GCTCC 3 cut(s) 68, 455, 917
Lsp1109I GCAGC 4 cut(s) 137, 229, 368, 649
MaeI CTAG 2 cut(s) 935, 991
MaeII ACGT 1 cut(s) 762
MaeIII GTNAC 2 cut(s) 84, 937
MboII GAAGA 7 cut(s) 160, 368, 560, 603, 660, 742, 779
MhlI GDGCHC 1 cut(s) 65
MluCI AATT 7 cut(s) 9, 163, 190, 575, 712, 732, 955
MmeI TCCRAC 3 cut(s) 38, 411, 542
MnlI CCTC 4 cut(s) 435, 470, 808, 842
Mph1103I ATGCAT 1 cut(s) 203
MroXI GAANNNNTTC 1 cut(s) 552
MseI TTAA 4 cut(s) 234, 266, 317, 578
MslI CAYNNNNRTG 2 cut(s) 200, 894
MspI CCGG 1 cut(s) 812
MspR9I CCNGG 2 cut(s) 291, 812
Mva1269I GAATGC 3 cut(s) 494, 912, 972
MvaI CCWGG 1 cut(s) 291
MwoI GCNNNNNNNGC 1 cut(s) 223
NciI CCSGG 1 cut(s) 812
NdeI CATATG 1 cut(s) 197
NlaIII CATG 4 cut(s) 27, 205, 621, 652
NlaIV GGNNCC 3 cut(s) 344, 435, 694
NmuCI GTSAC 1 cut(s) 84
NsiI ATGCAT 1 cut(s) 203
NspI RCATGY 1 cut(s) 27
PctI GAATGC 3 cut(s) 494, 912, 972
PdmI GAANNNNTTC 1 cut(s) 552
PfeI GAWTC 5 cut(s) 90, 110, 228, 608, 767
PflMI CCANNNNNTGG 2 cut(s) 352, 689
PfoI TCCNGGA 1 cut(s) 810
PkrI GCNGC 4 cut(s) 127, 219, 383, 664
Psp124BI GAGCTC 1 cut(s) 65
Psp1406I AACGTT 1 cut(s) 762
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 3 cut(s) 344, 435, 694
PstI CTGCAG 1 cut(s) 386
RsaI GTAC 4 cut(s) 186, 502, 694, 882
RsaNI GTAC 4 cut(s) 185, 501, 693, 881
RseI CAYNNNNRTG 2 cut(s) 200, 894
SacI GAGCTC 1 cut(s) 65
SaqAI TTAA 4 cut(s) 234, 266, 317, 578
SatI GCNGC 4 cut(s) 126, 218, 382, 663
ScaI AGTACT 1 cut(s) 502
ScrFI CCNGG 2 cut(s) 291, 812
SduI GDGCHC 1 cut(s) 65
SfcI CTRYAG 2 cut(s) 382, 622
SmiMI CAYNNNNRTG 2 cut(s) 200, 894
SmlI CTYRAG 1 cut(s) 307
SmoI CTYRAG 1 cut(s) 307
Sse9I AATT 7 cut(s) 9, 163, 190, 575, 712, 732, 955
SspMI CTAG 2 cut(s) 935, 991
SstI GAGCTC 1 cut(s) 65
StyD4I CCNGG 2 cut(s) 289, 810
StyI CCWWGG 1 cut(s) 338
TaiI ACGT 1 cut(s) 765
TasI AATT 7 cut(s) 9, 163, 190, 575, 712, 732, 955
TatI WGTACW 3 cut(s) 184, 500, 880
TfiI GAWTC 5 cut(s) 90, 110, 228, 608, 767
Tru1I TTAA 4 cut(s) 234, 266, 317, 578
Tru9I TTAA 4 cut(s) 234, 266, 317, 578
TscAI CASTG 2 cut(s) 127, 760
TseFI GTSAC 1 cut(s) 84
TseI GCWGC 4 cut(s) 125, 217, 381, 662
Tsp45I GTSAC 1 cut(s) 84
TspDTI ATGAA 6 cut(s) 160, 218, 339, 381, 548, 637
TspRI CASTG 2 cut(s) 127, 760
Van91I CCANNNNNTGG 2 cut(s) 352, 689
XapI RAATTY 1 cut(s) 732
XceI RCATGY 1 cut(s) 27
XcmI CCANNNNNNNNNTGG 2 cut(s) 686, 703
XmnI GAANNNNTTC 1 cut(s) 552
XspI CTAG 2 cut(s) 935, 991
ZrmI AGTACT 1 cut(s) 502
Zsp2I ATGCAT 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.