Rw4G025600

Squamosa promoter-binding-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0008130
Physical Location & Seq
Forward (+)
51738293 .. 51739726
1434 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G025600.1

Sequence Viewer

Length: 723 bp
ATGAAATCTAATCAGCAGCATTTGCGATTCTTTAAAATCCCTAATCCATTGGACATACATCAGTTAATGTTTTTCTTTCCACATCACCCTGGTTTCTTTCCTTGCTCAAGTCTATTAACATCTAATCTTACATTACCAAGGTTCCATTCATTGGAAGAGTTTGATGAAGGAAAAAGAAGCTGCAGAAGGCGTCTTGCTGGACACAACAAACGCCGAAGGAAGCCTCAGTTGGAACCTATGCCCACAAGTTTAGGAGCATTTCTTTCTGATTACCGAGGCGCAACATTTTCAGCATTCAGTACTCCACAACTCTATCCAACAAATGCTGTAATGAACTCTGCTTGGGAAGATTTTCCAAATCACAGCCACTCCCAATTAAGTGTGTCAAGAAGAAACTCATTTTCAGAATCTTTACCATGCTATAGCCACAGAGTAGGCATTGGCTTCATGCACTCTGAAGCTGCTTCTTCTGTTTGCCAACCACTTCTTGGTACCAACTTTGCATTGGGAAATTATGGCAACAACCACAAAATTTTCTCCAATGAGTTCTTCAGTGGCAACATTGAATCAAGTTGCGCTCGCTCTCTTCTGTCATTGACACCAGCAGTCCCGGAGGAGACTGGTTTGAGCCTCATAGGACAGACAAGTTCAATCTGCTCAGCTCAGTCCTTCGTCCTGTACTTCACCACTACAATGATTTGGGAATGCAGGGTGGAGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SBP

Protein Analysis

240

Amino Acids

27.1

Weight (kDa)

8.73

Isoelectric Point (pI)

60.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 47 - 74 3.6e-09 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 491
AccB1I GGYRCC 1 cut(s) 491
AccB7I CCANNNNNTGG 2 cut(s) 151, 488
AcsI RAATTY 1 cut(s) 531
AcuI CTGAAG 2 cut(s) 477, 535
AcyI GRCGYC 1 cut(s) 190
AfaI GTAC 3 cut(s) 301, 493, 680
AfiI CCNNNNNNNGG 2 cut(s) 151, 488
AgsI TTSAA 2 cut(s) 566, 651
AjnI CCWGG 1 cut(s) 88
AjuI GAANNNNNNNTTGG 6 cut(s) 130, 162, 212, 244, 533, 565
AluBI AGCT 3 cut(s) 180, 461, 662
AluI AGCT 3 cut(s) 180, 461, 662
Alw26I GTCTC 1 cut(s) 611
ApeKI GCWGC 3 cut(s) 16, 180, 461
ApoI RAATTY 1 cut(s) 531
Asp700I GAANNNNTTC 1 cut(s) 351
Asp718I GGTACC 1 cut(s) 491
AspLEI GCGC 2 cut(s) 281, 578
AsuC2I CCSGG 1 cut(s) 611
AsuHPI GGTGA 2 cut(s) 77, 676
BanI GGYRCC 1 cut(s) 491
BbvI GCAGC 3 cut(s) 28, 167, 448
BciT130I CCWGG 1 cut(s) 90
BcnI CCSGG 1 cut(s) 611
BcoDI GTCTC 1 cut(s) 611
BfmI CTRYAG 2 cut(s) 181, 421
BisI GCNGC 3 cut(s) 17, 181, 462
BlpI GCTNAGC 1 cut(s) 658
BlsI GCNGC 3 cut(s) 18, 182, 463
BmcAI AGTACT 1 cut(s) 301
Bme1390I CCNGG 2 cut(s) 90, 611
BmiI GGNNCC 3 cut(s) 143, 234, 493
BmrFI CCNGG 2 cut(s) 90, 611
Bpu1102I GCTNAGC 1 cut(s) 658
BpuEI CTTGAG 1 cut(s) 91
BpuMI CCSGG 1 cut(s) 611
BsaHI GRCGYC 1 cut(s) 190
BsaJI CCNNGG 3 cut(s) 88, 137, 274
BsaXI ACNNNNNCTCC 2 cut(s) 353, 383
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 488
Bse1I ACTGG 1 cut(s) 625
BseBI CCWGG 1 cut(s) 90
BseDI CCNNGG 3 cut(s) 88, 137, 274
BseLI CCNNNNNNNGG 2 cut(s) 151, 488
BseMII CTCAG 3 cut(s) 239, 672, 677
BseNI ACTGG 1 cut(s) 625
BseRI GAGGAG 1 cut(s) 629
BseXI GCAGC 3 cut(s) 28, 167, 448
BshNI GGYRCC 1 cut(s) 491
BsiSI CCGG 1 cut(s) 611
BslFI GGGAC 1 cut(s) 593
BslI CCNNNNNNNGG 2 cut(s) 151, 488
BsmAI GTCTC 1 cut(s) 611
BsmFI GGGAC 1 cut(s) 593
BsmI GAATGC 2 cut(s) 293, 710
Bsp1720I GCTNAGC 1 cut(s) 658
BspCNI CTCAG 3 cut(s) 238, 671, 676
BspLI GGNNCC 3 cut(s) 143, 234, 493
BspMAI CTGCAG 1 cut(s) 185
BspT107I GGYRCC 1 cut(s) 491
BsrI ACTGG 1 cut(s) 625
BssECI CCNNGG 3 cut(s) 88, 137, 274
BssNI GRCGYC 1 cut(s) 190
BssT1I CCWWGG 1 cut(s) 137
Bst2UI CCWGG 1 cut(s) 90
Bst6I CTCTTC 2 cut(s) 150, 591
BstACI GRCGYC 1 cut(s) 190
BstAPI GCANNNNNTGC 1 cut(s) 22
BstC8I GCNNGC 1 cut(s) 580
BstDEI CTNAG 3 cut(s) 225, 658, 663
BstHHI GCGC 2 cut(s) 281, 578
BstMAI GTCTC 1 cut(s) 611
BstMWI GCNNNNNNNGC 1 cut(s) 22
BstNI CCWGG 1 cut(s) 90
BstSCI CCNGG 2 cut(s) 88, 609
BstSFI CTRYAG 2 cut(s) 181, 421
BstV1I GCAGC 3 cut(s) 28, 167, 448
BtsIMutI CAGTG 1 cut(s) 559
Cac8I GCNNGC 1 cut(s) 580
CfoI GCGC 2 cut(s) 281, 578
CseI GACGC 1 cut(s) 179
Csp6I GTAC 3 cut(s) 300, 492, 679
CviAII CATG 2 cut(s) 417, 448
CviJI RGCY 8 cut(s) 180, 223, 366, 426, 444, 461, 630, 662
CviKI_1 RGCY 8 cut(s) 180, 223, 366, 426, 444, 461, 630, 662
CviQI GTAC 3 cut(s) 300, 492, 679
DdeI CTNAG 3 cut(s) 225, 658, 663
DraI TTTAAA 1 cut(s) 34
Eam1104I CTCTTC 2 cut(s) 150, 591
EarI CTCTTC 2 cut(s) 150, 591
Eco130I CCWWGG 1 cut(s) 137
Eco57I CTGAAG 2 cut(s) 477, 535
EcoRII CCWGG 1 cut(s) 88
EcoT14I CCWWGG 1 cut(s) 137
ErhI CCWWGG 1 cut(s) 137
FaeI CATG 2 cut(s) 420, 451
FaiI YATR 7 cut(s) 56, 239, 418, 423, 449, 516, 635
FaqI GGGAC 1 cut(s) 593
FatI CATG 2 cut(s) 416, 447
Fnu4HI GCNGC 3 cut(s) 17, 181, 462
Fsp4HI GCNGC 3 cut(s) 17, 181, 462
GlaI GCGC 2 cut(s) 280, 577
GluI GCNGC 3 cut(s) 17, 181, 462
HapII CCGG 1 cut(s) 611
HgaI GACGC 1 cut(s) 179
HhaI GCGC 2 cut(s) 281, 578
Hin1I GRCGYC 1 cut(s) 190
Hin1II CATG 2 cut(s) 420, 451
Hin6I GCGC 2 cut(s) 279, 576
HinP1I GCGC 2 cut(s) 279, 576
HinfI GANTC 3 cut(s) 27, 407, 566
HpaII CCGG 1 cut(s) 611
HphI GGTGA 2 cut(s) 77, 676
Hpy188I TCNGA 3 cut(s) 268, 406, 457
Hpy188III TCNNGA 1 cut(s) 387
HpyAV CCTTC 4 cut(s) 161, 180, 210, 679
HpyCH4V TGCA 4 cut(s) 183, 451, 503, 708
HpyF10VI GCNNNNNNNGC 1 cut(s) 22
HpyF3I CTNAG 3 cut(s) 225, 658, 663
Hsp92I GRCGYC 1 cut(s) 190
Hsp92II CATG 2 cut(s) 420, 451
HspAI GCGC 2 cut(s) 279, 576
KpnI GGTACC 1 cut(s) 495
LmnI GCTCC 2 cut(s) 254, 715
LpnPI CCDG 8 cut(s) 75, 102, 183, 606, 615, 624, 689, 694
Lsp1109I GCAGC 3 cut(s) 28, 167, 448
MboII GAAGA 6 cut(s) 167, 359, 402, 459, 541, 578
MluCI AATT 3 cut(s) 374, 511, 531
MmeI TCCRAC 2 cut(s) 210, 341
MnlI CCTC 4 cut(s) 234, 269, 607, 641
MroXI GAANNNNTTC 1 cut(s) 351
MseI TTAA 4 cut(s) 33, 65, 116, 377
MslI CAYNNNNRTG 1 cut(s) 692
MspI CCGG 1 cut(s) 611
MspR9I CCNGG 2 cut(s) 90, 611
Mva1269I GAATGC 2 cut(s) 293, 710
MvaI CCWGG 1 cut(s) 90
MwoI GCNNNNNNNGC 1 cut(s) 22
NciI CCSGG 1 cut(s) 611
NlaIII CATG 2 cut(s) 420, 451
NlaIV GGNNCC 3 cut(s) 143, 234, 493
PctI GAATGC 2 cut(s) 293, 710
PdmI GAANNNNTTC 1 cut(s) 351
PfeI GAWTC 3 cut(s) 27, 407, 566
PflMI CCANNNNNTGG 2 cut(s) 151, 488
PfoI TCCNGGA 1 cut(s) 609
PkrI GCNGC 3 cut(s) 18, 182, 463
Psp6I CCWGG 1 cut(s) 88
PspGI CCWGG 1 cut(s) 88
PspN4I GGNNCC 3 cut(s) 143, 234, 493
PstI CTGCAG 1 cut(s) 185
RsaI GTAC 3 cut(s) 301, 493, 680
RsaNI GTAC 3 cut(s) 300, 492, 679
RseI CAYNNNNRTG 1 cut(s) 692
SaqAI TTAA 4 cut(s) 33, 65, 116, 377
SatI GCNGC 3 cut(s) 17, 181, 462
ScaI AGTACT 1 cut(s) 301
ScrFI CCNGG 2 cut(s) 90, 611
SetI ASST 5 cut(s) 143, 182, 238, 463, 664
SfcI CTRYAG 2 cut(s) 181, 421
SmiMI CAYNNNNRTG 1 cut(s) 692
SmlI CTYRAG 1 cut(s) 106
SmoI CTYRAG 1 cut(s) 106
Sse9I AATT 3 cut(s) 374, 511, 531
StyD4I CCNGG 2 cut(s) 88, 609
StyI CCWWGG 1 cut(s) 137
TasI AATT 3 cut(s) 374, 511, 531
TatI WGTACW 2 cut(s) 299, 678
TfiI GAWTC 3 cut(s) 27, 407, 566
Tru1I TTAA 4 cut(s) 33, 65, 116, 377
Tru9I TTAA 4 cut(s) 33, 65, 116, 377
TscAI CASTG 1 cut(s) 559
TseI GCWGC 3 cut(s) 16, 180, 461
TspDTI ATGAA 5 cut(s) 17, 138, 180, 347, 436
TspRI CASTG 1 cut(s) 559
Van91I CCANNNNNTGG 2 cut(s) 151, 488
XapI RAATTY 1 cut(s) 531
XcmI CCANNNNNNNNNTGG 2 cut(s) 485, 502
XmnI GAANNNNTTC 1 cut(s) 351
ZrmI AGTACT 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.