FvH4_4g25210

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0017878
Physical Location & Seq
Forward (+)
27056812 .. 27057626
815 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g25210.t1

Sequence Viewer

Length: 519 bp
ATGAAGAGTTCAGTACGTCTAGCAGTACTTCTGGTATGTCTCATTGCTGTGTTCCTCCCCATAAGCCATTGCAGTGTGCCAAATGATTTCAAACTCGTCGAGGAAACATGCAAAAAAACATACCACTATGATCTCTGTCGCTTTGCTCTTAGCCGGGTGCCTGAGAGCGCCCATGCCGACGTCAAAGGCTTAGCCCTCATAATGGCTGATGTCGTACTCGGTCGAGCCCGGAATACAACCGTGAACAAAATCCAGGTGCTGCGTAAGAGTAGCCCAGGAGACAAACGCTTCGATGATTGTTTGCTACATTACACAGCAATAGAATTTGACATCGCACATTTTGACGAACATTTTCGTAATCACGACTATAAGTCGGCAGAGGCAACCATGGAAGATGCTTTCTCTCAAGCTGGAAACTGCGAACTTCTTTTTGATCGGCCGAAATCATCTCCTCTGCATGAGTATAACATACTTGTGACTCATCTTGCTGAATTGGCTGGAAGTATAGTTCTTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

173

Amino Acids

19.35

Weight (kDa)

6.29

Isoelectric Point (pI)

44.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 31 - 166 1.9e-13 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017878)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17150 AT3G17152
fragaria_vesca FvH4_4g25210
rosa_chinensis RchiOBHm_Chr4g0432401
rosa_multiflora Rmu_co8060982.1_g000001 Rmu_sc0001084.1_g000025 Rmu_sc0016445.1_g000009
rosa_rugosa Rorug04G0258200
rosa_samantha Rh4BG321700 Rh4CG336800 Rh4DG317300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 183
AccB1I GGYRCC 1 cut(s) 157
AcoI YGGCCR 1 cut(s) 437
AcsI RAATTY 1 cut(s) 323
AcyI GRCGYC 1 cut(s) 180
AfaI GTAC 3 cut(s) 15, 27, 216
AfiI CCNNNNNNNGG 1 cut(s) 202
AgsI TTSAA 1 cut(s) 91
AhdI GACNNNNNGTC 1 cut(s) 370
AjnI CCWGG 2 cut(s) 252, 274
AloI GAACNNNNNNTCC 1 cut(s) 492
AluBI AGCT 1 cut(s) 410
AluI AGCT 1 cut(s) 410
Alw26I GTCTC 2 cut(s) 44, 273
AlwNI CAGNNNCTG 1 cut(s) 259
AoxI GGCC 1 cut(s) 437
ApeKI GCWGC 1 cut(s) 259
ApoI RAATTY 1 cut(s) 323
ArsI GACNNNNNNTTYG 2 cut(s) 272, 304
Asp700I GAANNNNTTC 1 cut(s) 351
AspLEI GCGC 1 cut(s) 170
AsuC2I CCSGG 2 cut(s) 155, 229
BanI GGYRCC 1 cut(s) 157
BanII GRGCYC 1 cut(s) 229
BbvI GCAGC 1 cut(s) 246
BciT130I CCWGG 2 cut(s) 254, 276
BcnI CCSGG 2 cut(s) 155, 229
BcoDI GTCTC 2 cut(s) 44, 273
BfaI CTAG 1 cut(s) 20
BfoI RGCGCY 1 cut(s) 171
BisI GCNGC 1 cut(s) 260
BlpI GCTNAGC 1 cut(s) 190
BlsI GCNGC 1 cut(s) 261
BmcAI AGTACT 1 cut(s) 27
Bme1390I CCNGG 4 cut(s) 155, 229, 254, 276
BmeRI GACNNNNNGTC 1 cut(s) 370
BmiI GGNNCC 1 cut(s) 159
BmrFI CCNGG 4 cut(s) 155, 229, 254, 276
BmsI GCATC 1 cut(s) 385
Bpu1102I GCTNAGC 1 cut(s) 190
BpuEI CTTGAG 1 cut(s) 390
BpuMI CCSGG 2 cut(s) 155, 229
BsaHI GRCGYC 1 cut(s) 180
BsaJI CCNNGG 2 cut(s) 274, 387
Bsc4I CCNNNNNNNGG 1 cut(s) 202
Bse3DI GCAATG 2 cut(s) 42, 67
BseBI CCWGG 2 cut(s) 254, 276
BseDI CCNNGG 2 cut(s) 274, 387
BseLI CCNNNNNNNGG 1 cut(s) 202
BseMI GCAATG 2 cut(s) 42, 67
BseMII CTCAG 1 cut(s) 153
BseRI GAGGAG 1 cut(s) 441
BseX3I CGGCCG 1 cut(s) 437
BseXI GCAGC 1 cut(s) 246
Bsh1285I CGRYCG 2 cut(s) 223, 440
BshFI GGCC 1 cut(s) 439
BshNI GGYRCC 1 cut(s) 157
BsiEI CGRYCG 2 cut(s) 223, 440
BsiSI CCGG 2 cut(s) 154, 229
BslI CCNNNNNNNGG 1 cut(s) 202
BsmAI GTCTC 2 cut(s) 44, 273
BsnI GGCC 1 cut(s) 439
Bsp1286I GDGCHC 1 cut(s) 229
Bsp143I GATC 2 cut(s) 130, 433
Bsp1720I GCTNAGC 1 cut(s) 190
Bsp19I CCATGG 1 cut(s) 387
BspANI GGCC 1 cut(s) 439
BspCNI CTCAG 1 cut(s) 154
BspLI GGNNCC 1 cut(s) 159
BspT107I GGYRCC 1 cut(s) 157
BsrDI GCAATG 2 cut(s) 42, 67
BssECI CCNNGG 2 cut(s) 274, 387
BssMI GATC 2 cut(s) 130, 433
BssNI GRCGYC 1 cut(s) 180
BssT1I CCWWGG 1 cut(s) 387
Bst2UI CCWGG 2 cut(s) 254, 276
Bst4CI ACNGT 1 cut(s) 241
BstACI GRCGYC 1 cut(s) 180
BstDEI CTNAG 3 cut(s) 149, 162, 190
BstDSI CCRYGG 1 cut(s) 387
BstH2I RGCGCY 1 cut(s) 171
BstHHI GCGC 1 cut(s) 170
BstKTI GATC 2 cut(s) 133, 436
BstMAI GTCTC 2 cut(s) 44, 273
BstMBI GATC 2 cut(s) 130, 433
BstMCI CGRYCG 2 cut(s) 223, 440
BstMWI GCNNNNNNNGC 1 cut(s) 494
BstNI CCWGG 2 cut(s) 254, 276
BstNSI RCATGY 1 cut(s) 111
BstSCI CCNGG 4 cut(s) 153, 227, 252, 274
BstV1I GCAGC 1 cut(s) 246
BstZI CGGCCG 1 cut(s) 437
BsuRI GGCC 1 cut(s) 439
BtgI CCRYGG 1 cut(s) 387
BtgZI GCGATG 1 cut(s) 316
BtsI GCAGTG 1 cut(s) 79
BtsIMutI CAGTG 1 cut(s) 79
CaiI CAGNNNCTG 1 cut(s) 259
CfoI GCGC 1 cut(s) 170
Csp6I GTAC 3 cut(s) 14, 26, 215
CviAII CATG 4 cut(s) 108, 173, 388, 458
CviQI GTAC 3 cut(s) 14, 26, 215
DdeI CTNAG 3 cut(s) 149, 162, 190
DpnI GATC 2 cut(s) 132, 435
DpnII GATC 2 cut(s) 130, 433
DriI GACNNNNNGTC 1 cut(s) 370
EaeI YGGCCR 1 cut(s) 437
EagI CGGCCG 1 cut(s) 437
Eam1105I GACNNNNNGTC 1 cut(s) 370
EclXI CGGCCG 1 cut(s) 437
Eco130I CCWWGG 1 cut(s) 387
Eco24I GRGCYC 1 cut(s) 229
Eco52I CGGCCG 1 cut(s) 437
EcoRII CCWGG 2 cut(s) 252, 274
EcoT14I CCWWGG 1 cut(s) 387
EcoT38I GRGCYC 1 cut(s) 229
ErhI CCWWGG 1 cut(s) 387
FaeI CATG 4 cut(s) 111, 176, 391, 461
FatI CATG 4 cut(s) 107, 172, 387, 457
Fnu4HI GCNGC 1 cut(s) 260
FriOI GRGCYC 1 cut(s) 229
Fsp4HI GCNGC 1 cut(s) 260
FspBI CTAG 1 cut(s) 20
GlaI GCGC 1 cut(s) 169
GluI GCNGC 1 cut(s) 260
HaeII RGCGCY 1 cut(s) 171
HaeIII GGCC 1 cut(s) 439
HapII CCGG 2 cut(s) 154, 229
HhaI GCGC 1 cut(s) 170
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 4 cut(s) 111, 176, 391, 461
Hin6I GCGC 1 cut(s) 168
HinP1I GCGC 1 cut(s) 168
HinfI GANTC 1 cut(s) 478
HpaII CCGG 2 cut(s) 154, 229
Hpy166II GTNNAC 1 cut(s) 244
Hpy188III TCNNGA 1 cut(s) 362
Hpy8I GTNNAC 1 cut(s) 244
Hpy99I CGWCG 2 cut(s) 101, 182
HpyCH4III ACNGT 1 cut(s) 241
HpyCH4IV ACGT 2 cut(s) 16, 180
HpyCH4V TGCA 3 cut(s) 72, 111, 457
HpyF10VI GCNNNNNNNGC 1 cut(s) 494
HpyF3I CTNAG 3 cut(s) 149, 162, 190
HpySE526I ACGT 2 cut(s) 16, 180
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 4 cut(s) 111, 176, 391, 461
HspAI GCGC 1 cut(s) 168
Kzo9I GATC 2 cut(s) 130, 433
Lsp1109I GCAGC 1 cut(s) 246
LweI GCATC 1 cut(s) 385
MaeI CTAG 1 cut(s) 20
MaeII ACGT 2 cut(s) 16, 180
MaeIII GTNAC 1 cut(s) 475
MalI GATC 2 cut(s) 132, 435
MboI GATC 2 cut(s) 130, 433
MboII GAAGA 2 cut(s) 16, 404
MhlI GDGCHC 1 cut(s) 229
MluCI AATT 2 cut(s) 323, 491
MlyI GAGTC 1 cut(s) 472
MnlI CCTC 5 cut(s) 65, 94, 206, 373, 462
MroXI GAANNNNTTC 1 cut(s) 351
MslI CAYNNNNRTG 3 cut(s) 47, 72, 473
MspI CCGG 2 cut(s) 154, 229
MspR9I CCNGG 4 cut(s) 155, 229, 254, 276
MvaI CCWGG 2 cut(s) 254, 276
MwoI GCNNNNNNNGC 1 cut(s) 494
NciI CCSGG 2 cut(s) 155, 229
NcoI CCATGG 1 cut(s) 387
NdeII GATC 2 cut(s) 130, 433
NlaIII CATG 4 cut(s) 111, 176, 391, 461
NlaIV GGNNCC 1 cut(s) 159
NmuCI GTSAC 1 cut(s) 475
NspI RCATGY 1 cut(s) 111
PdmI GAANNNNTTC 1 cut(s) 351
PkrI GCNGC 1 cut(s) 261
PleI GAGTC 1 cut(s) 472
PpsI GAGTC 1 cut(s) 472
Psp6I CCWGG 2 cut(s) 252, 274
PspGI CCWGG 2 cut(s) 252, 274
PspN4I GGNNCC 1 cut(s) 159
PstNI CAGNNNCTG 1 cut(s) 259
RsaI GTAC 3 cut(s) 15, 27, 216
RsaNI GTAC 3 cut(s) 14, 26, 215
RseI CAYNNNNRTG 3 cut(s) 47, 72, 473
SatI GCNGC 1 cut(s) 260
Sau3AI GATC 2 cut(s) 130, 433
ScaI AGTACT 1 cut(s) 27
SchI GAGTC 1 cut(s) 472
ScrFI CCNGG 4 cut(s) 155, 229, 254, 276
SduI GDGCHC 1 cut(s) 229
SetI ASST 4 cut(s) 19, 183, 258, 412
SfaNI GCATC 1 cut(s) 385
SmiMI CAYNNNNRTG 3 cut(s) 47, 72, 473
SmlI CTYRAG 1 cut(s) 405
SmoI CTYRAG 1 cut(s) 405
Sse9I AATT 2 cut(s) 323, 491
SspMI CTAG 1 cut(s) 20
StyD4I CCNGG 4 cut(s) 153, 227, 252, 274
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 1 cut(s) 241
TaiI ACGT 2 cut(s) 19, 183
TaqI TCGA 3 cut(s) 99, 223, 291
TaqII GACCGA 1 cut(s) 209
TasI AATT 2 cut(s) 323, 491
TatI WGTACW 1 cut(s) 25
TscAI CASTG 1 cut(s) 79
TseFI GTSAC 1 cut(s) 475
TseI GCWGC 1 cut(s) 259
Tsp45I GTSAC 1 cut(s) 475
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 79
XapI RAATTY 1 cut(s) 323
XceI RCATGY 1 cut(s) 111
XmnI GAANNNNTTC 1 cut(s) 351
XspI CTAG 1 cut(s) 20
ZraI GACGTC 1 cut(s) 181
ZrmI AGTACT 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.