Rh4BG321700

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0017878
Physical Location & Seq
Forward (+)
48746443 .. 48746961
519 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG321700.1

Sequence Viewer

Length: 519 bp
ATGAAGAGTTCAGTACGTCTAGCAAGTTTCGTATGTCTCATTCATGTTGTGTTTCTCTCCATTAGCCATTGCAGGGTTTTCCTGCCGAACGATCTCAGGCTTATTGAGCATACATGCAACAAAACACCACACCGCGATCTCTGTGTCTCTGCTCTTACCTCGGACCCTGAGAGTGCCGGTGCAGATGTCAAGGGCTTAGCCCGCATAATGGCTAATGTTATACTTAGGAAAGCCACAGATGATACCCAGCACAAGATCGAGGGGATGATTGCGCATAACCCAGGAGACCAACGCTTAACCAAATGTAGAGATGATTACAAAGTAATCAAAACAAAAATTGACTCCACCAATGCATCTATTACTAAACACGATTATAAGTCGGCCGAGGAAAGCATGAATGATGCTATCGCTGAAGCTAATATATGCGAAGGTCGTTTTCCAGGCAGATCTCCTCTGATATATGAGAACAAACTTGAGGCTGATATTGCTGATTTAGCTGCTGACATAGTAATTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

172

Amino Acids

19.05

Weight (kDa)

6.37

Isoelectric Point (pI)

33.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 34 - 157 5.2e-17 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017878)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17150 AT3G17152
fragaria_vesca FvH4_4g25210
rosa_chinensis RchiOBHm_Chr4g0432401
rosa_multiflora Rmu_co8060982.1_g000001 Rmu_sc0001084.1_g000025 Rmu_sc0016445.1_g000009
rosa_rugosa Rorug04G0258200
rosa_samantha Rh4BG321700 Rh4CG336800 Rh4DG317300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 375
Acc16I TGCGCA 1 cut(s) 273
AccII CGCG 1 cut(s) 135
AciI CCGC 2 cut(s) 133, 202
AcoI YGGCCR 1 cut(s) 381
AcuI CTGAAG 1 cut(s) 432
AfaI GTAC 1 cut(s) 15
AfiI CCNNNNNNNGG 2 cut(s) 73, 208
AjnI CCWGG 2 cut(s) 280, 439
AluBI AGCT 2 cut(s) 416, 497
AluI AGCT 2 cut(s) 416, 497
Alw26I GTCTC 3 cut(s) 41, 151, 279
AoxI GGCC 1 cut(s) 381
ApeKI GCWGC 1 cut(s) 497
AspLEI GCGC 1 cut(s) 274
AspS9I GGNCC 1 cut(s) 163
AvaII GGWCC 1 cut(s) 163
BbvI GCAGC 1 cut(s) 484
BciT130I CCWGG 2 cut(s) 282, 441
BcoDI GTCTC 3 cut(s) 41, 151, 279
BfaI CTAG 1 cut(s) 20
BglII AGATCT 1 cut(s) 446
BisI GCNGC 1 cut(s) 498
BlpI GCTNAGC 1 cut(s) 196
BlsI GCNGC 1 cut(s) 499
Bme1390I CCNGG 2 cut(s) 282, 441
Bme18I GGWCC 1 cut(s) 163
BmgT120I GGNCC 1 cut(s) 163
BmiI GGNNCC 1 cut(s) 165
BmrFI CCNGG 2 cut(s) 282, 441
BmsI GCATC 2 cut(s) 362, 391
Bpu1102I GCTNAGC 1 cut(s) 196
BpuEI CTTGAG 1 cut(s) 494
BsaI GGTCTC 1 cut(s) 279
BsaJI CCNNGG 3 cut(s) 159, 280, 384
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 208
Bse118I RCCGGY 1 cut(s) 176
Bse3DI GCAATG 1 cut(s) 67
BseBI CCWGG 2 cut(s) 282, 441
BseDI CCNNGG 3 cut(s) 159, 280, 384
BseGI GGATG 1 cut(s) 270
BseLI CCNNNNNNNGG 2 cut(s) 73, 208
BseMI GCAATG 1 cut(s) 67
BseMII CTCAG 2 cut(s) 109, 159
BseRI GAGGAG 1 cut(s) 441
BseX3I CGGCCG 1 cut(s) 381
BseXI GCAGC 1 cut(s) 484
BseYI CCCAGC 1 cut(s) 246
BsgI GTGCAG 1 cut(s) 201
Bsh1236I CGCG 1 cut(s) 135
Bsh1285I CGRYCG 1 cut(s) 384
BshFI GGCC 1 cut(s) 383
BsiEI CGRYCG 1 cut(s) 384
BsiSI CCGG 1 cut(s) 177
BslI CCNNNNNNNGG 2 cut(s) 73, 208
BsmAI GTCTC 3 cut(s) 41, 151, 279
BsnI GGCC 1 cut(s) 383
Bso31I GGTCTC 1 cut(s) 279
Bsp143I GATC 4 cut(s) 91, 136, 255, 446
Bsp1720I GCTNAGC 1 cut(s) 196
BspACI CCGC 2 cut(s) 133, 202
BspANI GGCC 1 cut(s) 383
BspCNI CTCAG 2 cut(s) 108, 160
BspFNI CGCG 1 cut(s) 135
BspLI GGNNCC 1 cut(s) 165
BspTNI GGTCTC 1 cut(s) 279
BsrDI GCAATG 1 cut(s) 67
BsrFI RCCGGY 1 cut(s) 176
BssAI RCCGGY 1 cut(s) 176
BssECI CCNNGG 3 cut(s) 159, 280, 384
BssMI GATC 4 cut(s) 91, 136, 255, 446
Bst2UI CCWGG 2 cut(s) 282, 441
BstC8I GCNNGC 1 cut(s) 202
BstDEI CTNAG 4 cut(s) 95, 168, 196, 224
BstF5I GGATG 1 cut(s) 270
BstFNI CGCG 1 cut(s) 135
BstHHI GCGC 1 cut(s) 274
BstKTI GATC 4 cut(s) 94, 139, 258, 449
BstMAI GTCTC 3 cut(s) 41, 151, 279
BstMBI GATC 4 cut(s) 91, 136, 255, 446
BstMCI CGRYCG 1 cut(s) 384
BstMWI GCNNNNNNNGC 4 cut(s) 106, 201, 485, 494
BstNI CCWGG 2 cut(s) 282, 441
BstNSI RCATGY 1 cut(s) 117
BstSCI CCNGG 2 cut(s) 280, 439
BstUI CGCG 1 cut(s) 135
BstV1I GCAGC 1 cut(s) 484
BstX2I RGATCY 1 cut(s) 446
BstYI RGATCY 1 cut(s) 446
BstZI CGGCCG 1 cut(s) 381
BsuRI GGCC 1 cut(s) 383
BtsCI GGATG 1 cut(s) 270
Cac8I GCNNGC 1 cut(s) 202
CfoI GCGC 1 cut(s) 274
Cfr10I RCCGGY 1 cut(s) 176
Cfr13I GGNCC 1 cut(s) 163
Csp6I GTAC 1 cut(s) 14
CviAII CATG 3 cut(s) 44, 114, 394
CviQI GTAC 1 cut(s) 14
DdeI CTNAG 4 cut(s) 95, 168, 196, 224
DpnI GATC 4 cut(s) 93, 138, 257, 448
DpnII GATC 4 cut(s) 91, 136, 255, 446
EaeI YGGCCR 1 cut(s) 381
EagI CGGCCG 1 cut(s) 381
EclXI CGGCCG 1 cut(s) 381
Eco31I GGTCTC 1 cut(s) 279
Eco47I GGWCC 1 cut(s) 163
Eco52I CGGCCG 1 cut(s) 381
Eco57I CTGAAG 1 cut(s) 432
EcoRII CCWGG 2 cut(s) 280, 439
EcoT22I ATGCAT 1 cut(s) 355
FaeI CATG 3 cut(s) 47, 117, 397
FatI CATG 3 cut(s) 43, 113, 393
FauI CCCGC 1 cut(s) 209
Fnu4HI GCNGC 1 cut(s) 498
FokI GGATG 1 cut(s) 277
Fsp4HI GCNGC 1 cut(s) 498
FspBI CTAG 1 cut(s) 20
FspI TGCGCA 1 cut(s) 273
GlaI GCGC 1 cut(s) 273
GluI GCNGC 1 cut(s) 498
GsaI CCCAGC 1 cut(s) 250
HaeIII GGCC 1 cut(s) 383
HapII CCGG 1 cut(s) 177
HhaI GCGC 1 cut(s) 274
Hin1II CATG 3 cut(s) 47, 117, 397
Hin6I GCGC 1 cut(s) 272
HinP1I GCGC 1 cut(s) 272
HinfI GANTC 1 cut(s) 341
HpaII CCGG 1 cut(s) 177
Hpy188I TCNGA 2 cut(s) 163, 456
HpyAV CCTTC 1 cut(s) 422
HpyCH4IV ACGT 1 cut(s) 16
HpyCH4V TGCA 4 cut(s) 72, 117, 182, 353
HpyF10VI GCNNNNNNNGC 4 cut(s) 106, 201, 485, 494
HpyF3I CTNAG 4 cut(s) 95, 168, 196, 224
HpySE526I ACGT 1 cut(s) 16
Hsp92II CATG 3 cut(s) 47, 117, 397
HspAI GCGC 1 cut(s) 272
Kzo9I GATC 4 cut(s) 91, 136, 255, 446
Lsp1109I GCAGC 1 cut(s) 484
LweI GCATC 2 cut(s) 362, 391
MaeI CTAG 1 cut(s) 20
MaeII ACGT 1 cut(s) 16
MalI GATC 4 cut(s) 93, 138, 257, 448
MboI GATC 4 cut(s) 91, 136, 255, 446
MboII GAAGA 1 cut(s) 16
MflI RGATCY 1 cut(s) 446
MluCI AATT 2 cut(s) 336, 510
MlyI GAGTC 1 cut(s) 335
MnlI CCTC 5 cut(s) 169, 253, 379, 462, 469
Mph1103I ATGCAT 1 cut(s) 355
MseI TTAA 1 cut(s) 296
MspI CCGG 1 cut(s) 177
MspR9I CCNGG 2 cut(s) 282, 441
MvaI CCWGG 2 cut(s) 282, 441
MvnI CGCG 1 cut(s) 135
MwoI GCNNNNNNNGC 4 cut(s) 106, 201, 485, 494
NdeII GATC 4 cut(s) 91, 136, 255, 446
NlaIII CATG 3 cut(s) 47, 117, 397
NlaIV GGNNCC 1 cut(s) 165
NmeAIII GCCGAG 1 cut(s) 409
NsbI TGCGCA 1 cut(s) 273
NsiI ATGCAT 1 cut(s) 355
NspI RCATGY 1 cut(s) 117
PkrI GCNGC 1 cut(s) 499
PleI GAGTC 1 cut(s) 335
PpsI GAGTC 1 cut(s) 335
PsiI TTATAA 1 cut(s) 375
Psp6I CCWGG 2 cut(s) 280, 439
PspFI CCCAGC 1 cut(s) 246
PspGI CCWGG 2 cut(s) 280, 439
PspN4I GGNNCC 1 cut(s) 165
PspPI GGNCC 1 cut(s) 163
PsuI RGATCY 1 cut(s) 446
RsaI GTAC 1 cut(s) 15
RsaNI GTAC 1 cut(s) 14
SaqAI TTAA 1 cut(s) 296
SatI GCNGC 1 cut(s) 498
Sau3AI GATC 4 cut(s) 91, 136, 255, 446
Sau96I GGNCC 1 cut(s) 163
SchI GAGTC 1 cut(s) 335
ScrFI CCNGG 2 cut(s) 282, 441
SetI ASST 5 cut(s) 19, 161, 418, 433, 499
SfaNI GCATC 2 cut(s) 362, 391
SinI GGWCC 1 cut(s) 163
SmlI CTYRAG 1 cut(s) 473
SmoI CTYRAG 1 cut(s) 473
Sse9I AATT 2 cut(s) 336, 510
SsiI CCGC 2 cut(s) 133, 202
SspMI CTAG 1 cut(s) 20
StyD4I CCNGG 2 cut(s) 280, 439
TaiI ACGT 1 cut(s) 19
TaqI TCGA 1 cut(s) 258
TasI AATT 2 cut(s) 336, 510
Tru1I TTAA 1 cut(s) 296
Tru9I TTAA 1 cut(s) 296
TseI GCWGC 1 cut(s) 497
TspDTI ATGAA 3 cut(s) 17, 32, 410
VpaK11BI GGWCC 1 cut(s) 163
XceI RCATGY 1 cut(s) 117
XspI CTAG 1 cut(s) 20
Zsp2I ATGCAT 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.