Rorug04G0258200

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0017878
Physical Location & Seq
Forward (+)
43139376 .. 43141721
2346 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0258200.1

Sequence Viewer

Length: 348 bp
ATGGCAAGCTCCTCCTCCTCACTGCTACTCTTCGGCTTCATCTTCCTCTGTTCAATCCTGGCCGCCCATTCCACTCTCGCCGCCGCTTCCACTTCCGCCGTCGATTTCAGCTGGGGCTCCGCCGGCGGAGGGCTGATAGGTGATTTGGATGAGGAGCTGGAGCTGCAGATGGACTCGGAGATCAACCGGCGCATCTTAGCCACCACCAGGTACATTAGCTACGGTGCGCTGAGGAGGAACACTGTCCCCTGCTCGCGACGCGGCGCCTCGTACTACAATTGCAGGCCCGGAGCTCAGGCCAACCCCTACTCGCGCGGCTGTAGCCGCATCACTCGCTGCAGGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

115

Amino Acids

12.45

Weight (kDa)

9.01

Isoelectric Point (pI)

52.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 54 - 114 1.7e-29 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017878)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17150 AT3G17152
fragaria_vesca FvH4_4g25210
rosa_chinensis RchiOBHm_Chr4g0432401
rosa_multiflora Rmu_co8060982.1_g000001 Rmu_sc0001084.1_g000025 Rmu_sc0016445.1_g000009
rosa_rugosa Rorug04G0258200
rosa_samantha Rh4BG321700 Rh4CG336800 Rh4DG317300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 263
AccII CGCG 4 cut(s) 256, 261, 313, 315
AciI CCGC 9 cut(s) 63, 81, 84, 96, 120, 126, 261, 315, 325
AcoI YGGCCR 1 cut(s) 60
AcyI GRCGYC 1 cut(s) 264
AfaI GTAC 2 cut(s) 212, 272
AfiI CCNNNNNNNGG 2 cut(s) 129, 207
AgsI TTSAA 1 cut(s) 54
AjnI CCWGG 2 cut(s) 57, 206
AluBI AGCT 6 cut(s) 9, 111, 157, 163, 219, 293
AluI AGCT 6 cut(s) 9, 111, 157, 163, 219, 293
Alw21I GWGCWC 1 cut(s) 295
AoxI GGCC 3 cut(s) 60, 284, 297
ApeKI GCWGC 2 cut(s) 163, 336
AspLEI GCGC 4 cut(s) 192, 229, 266, 315
AspS9I GGNCC 1 cut(s) 285
AsuC2I CCSGG 1 cut(s) 288
AsuHPI GGTGA 1 cut(s) 152
BanI GGYRCC 1 cut(s) 263
BanII GRGCYC 2 cut(s) 119, 295
Bbv12I GWGCWC 1 cut(s) 295
BbvCI CCTCAGC 1 cut(s) 230
BbvI GCAGC 2 cut(s) 150, 323
BccI CCATC 1 cut(s) 163
BceAI ACGGC 1 cut(s) 83
BciT130I CCWGG 2 cut(s) 59, 208
BcnI CCSGG 1 cut(s) 288
BfmI CTRYAG 3 cut(s) 164, 319, 337
BfoI RGCGCY 1 cut(s) 267
BisI GCNGC 8 cut(s) 63, 81, 84, 164, 262, 316, 325, 337
BlsI GCNGC 8 cut(s) 64, 82, 85, 165, 263, 317, 326, 338
Bme1390I CCNGG 3 cut(s) 59, 208, 288
BmgT120I GGNCC 1 cut(s) 285
BmiI GGNNCC 2 cut(s) 118, 265
BmrFI CCNGG 3 cut(s) 59, 208, 288
BmsI GCATC 2 cut(s) 201, 336
BpmI CTGGAG 1 cut(s) 179
Bpu10I CCTNAGC 2 cut(s) 230, 294
BpuMI CCSGG 1 cut(s) 288
BsaHI GRCGYC 1 cut(s) 264
Bsc4I CCNNNNNNNGG 2 cut(s) 129, 207
Bse118I RCCGGY 2 cut(s) 122, 186
BseBI CCWGG 2 cut(s) 59, 208
BseGI GGATG 1 cut(s) 154
BseLI CCNNNNNNNGG 2 cut(s) 129, 207
BseMII CTCAG 2 cut(s) 221, 308
BseRI GAGGAG 4 cut(s) 4, 7, 167, 247
BseXI GCAGC 2 cut(s) 150, 323
BseYI CCCAGC 1 cut(s) 111
Bsh1236I CGCG 4 cut(s) 256, 261, 313, 315
BshFI GGCC 3 cut(s) 62, 286, 299
BshNI GGYRCC 1 cut(s) 263
BsiHKAI GWGCWC 1 cut(s) 295
BsiSI CCGG 3 cut(s) 123, 187, 288
BslFI GGGAC 1 cut(s) 230
BslI CCNNNNNNNGG 2 cut(s) 129, 207
BsmFI GGGAC 1 cut(s) 230
BsnI GGCC 3 cut(s) 62, 286, 299
Bsp1286I GDGCHC 2 cut(s) 119, 295
Bsp143I GATC 1 cut(s) 180
Bsp68I TCGCGA 1 cut(s) 256
BspACI CCGC 9 cut(s) 63, 81, 84, 96, 120, 126, 261, 315, 325
BspANI GGCC 3 cut(s) 62, 286, 299
BspCNI CTCAG 2 cut(s) 222, 307
BspFNI CGCG 4 cut(s) 256, 261, 313, 315
BspLI GGNNCC 2 cut(s) 118, 265
BspMAI CTGCAG 2 cut(s) 168, 341
BspT107I GGYRCC 1 cut(s) 263
BsrFI RCCGGY 2 cut(s) 122, 186
BssAI RCCGGY 2 cut(s) 122, 186
BssMI GATC 1 cut(s) 180
BssNI GRCGYC 1 cut(s) 264
Bst2UI CCWGG 2 cut(s) 59, 208
Bst4CI ACNGT 2 cut(s) 224, 244
Bst6I CTCTTC 1 cut(s) 35
BstACI GRCGYC 1 cut(s) 264
BstC8I GCNNGC 5 cut(s) 7, 124, 254, 284, 341
BstDEI CTNAG 3 cut(s) 196, 230, 294
BstF5I GGATG 1 cut(s) 154
BstFNI CGCG 4 cut(s) 256, 261, 313, 315
BstH2I RGCGCY 1 cut(s) 267
BstHHI GCGC 4 cut(s) 192, 229, 266, 315
BstKTI GATC 1 cut(s) 183
BstMBI GATC 1 cut(s) 180
BstMWI GCNNNNNNNGC 6 cut(s) 123, 163, 258, 321, 324, 333
BstNI CCWGG 2 cut(s) 59, 208
BstSCI CCNGG 3 cut(s) 57, 206, 286
BstSFI CTRYAG 3 cut(s) 164, 319, 337
BstUI CGCG 4 cut(s) 256, 261, 313, 315
BstV1I GCAGC 2 cut(s) 150, 323
BsuRI GGCC 3 cut(s) 62, 286, 299
BtsCI GGATG 1 cut(s) 154
BtsI GCAGTG 1 cut(s) 20
BtsIMutI CAGTG 2 cut(s) 20, 240
BtuMI TCGCGA 1 cut(s) 256
Cac8I GCNNGC 5 cut(s) 7, 124, 254, 284, 341
CfoI GCGC 4 cut(s) 192, 229, 266, 315
Cfr10I RCCGGY 2 cut(s) 122, 186
Cfr13I GGNCC 1 cut(s) 285
CseI GACGC 1 cut(s) 267
CsiI ACCWGGT 1 cut(s) 206
Csp6I GTAC 2 cut(s) 211, 271
CviQI GTAC 2 cut(s) 211, 271
DdeI CTNAG 3 cut(s) 196, 230, 294
DinI GGCGCC 1 cut(s) 265
DpnI GATC 1 cut(s) 182
DpnII GATC 1 cut(s) 180
EaeI YGGCCR 1 cut(s) 60
Eam1104I CTCTTC 1 cut(s) 35
EarI CTCTTC 1 cut(s) 35
EciI GGCGGA 3 cut(s) 85, 109, 141
Ecl136II GAGCTC 1 cut(s) 293
Eco24I GRGCYC 2 cut(s) 119, 295
Eco53kI GAGCTC 1 cut(s) 293
EcoICRI GAGCTC 1 cut(s) 293
EcoRII CCWGG 2 cut(s) 57, 206
EcoT38I GRGCYC 2 cut(s) 119, 295
EgeI GGCGCC 1 cut(s) 265
EheI GGCGCC 1 cut(s) 265
FaqI GGGAC 1 cut(s) 230
Fnu4HI GCNGC 8 cut(s) 63, 81, 84, 164, 262, 316, 325, 337
FokI GGATG 1 cut(s) 161
FriOI GRGCYC 2 cut(s) 119, 295
Fsp4HI GCNGC 8 cut(s) 63, 81, 84, 164, 262, 316, 325, 337
GlaI GCGC 4 cut(s) 191, 228, 265, 314
GluI GCNGC 8 cut(s) 63, 81, 84, 164, 262, 316, 325, 337
GsaI CCCAGC 1 cut(s) 115
GsuI CTGGAG 1 cut(s) 179
HaeII RGCGCY 1 cut(s) 267
HaeIII GGCC 3 cut(s) 62, 286, 299
HapII CCGG 3 cut(s) 123, 187, 288
HgaI GACGC 1 cut(s) 267
HhaI GCGC 4 cut(s) 192, 229, 266, 315
Hin1I GRCGYC 1 cut(s) 264
Hin6I GCGC 4 cut(s) 190, 227, 264, 313
HinP1I GCGC 4 cut(s) 190, 227, 264, 313
HinfI GANTC 1 cut(s) 173
HpaII CCGG 3 cut(s) 123, 187, 288
HphI GGTGA 1 cut(s) 152
Hpy188I TCNGA 1 cut(s) 178
Hpy188III TCNNGA 1 cut(s) 255
Hpy99I CGWCG 2 cut(s) 104, 261
HpyCH4III ACNGT 2 cut(s) 224, 244
HpyCH4V TGCA 3 cut(s) 166, 282, 339
HpyF10VI GCNNNNNNNGC 6 cut(s) 123, 163, 258, 321, 324, 333
HpyF3I CTNAG 3 cut(s) 196, 230, 294
Hsp92I GRCGYC 1 cut(s) 264
HspAI GCGC 4 cut(s) 190, 227, 264, 313
KasI GGCGCC 1 cut(s) 263
KroI GCCGGC 1 cut(s) 122
KroNI GCCGGC 1 cut(s) 124
Kzo9I GATC 1 cut(s) 180
LmnI GCTCC 5 cut(s) 14, 122, 154, 160, 290
Lsp1109I GCAGC 2 cut(s) 150, 323
LweI GCATC 2 cut(s) 201, 336
MabI ACCWGGT 1 cut(s) 206
MalI GATC 1 cut(s) 182
MboI GATC 1 cut(s) 180
MboII GAAGA 2 cut(s) 22, 34
MfeI CAATTG 1 cut(s) 277
MhlI GDGCHC 2 cut(s) 119, 295
MluCI AATT 1 cut(s) 277
Mly113I GGCGCC 1 cut(s) 264
MlyI GAGTC 1 cut(s) 167
MnlI CCTC 9 cut(s) 22, 25, 28, 56, 122, 145, 225, 228, 277
MreI CGCCGGCG 1 cut(s) 122
MroNI GCCGGC 1 cut(s) 122
MspA1I CMGCKG 1 cut(s) 111
MspI CCGG 3 cut(s) 123, 187, 288
MspR9I CCNGG 3 cut(s) 59, 208, 288
MunI CAATTG 1 cut(s) 277
MvaI CCWGG 2 cut(s) 59, 208
MvnI CGCG 4 cut(s) 256, 261, 313, 315
MwoI GCNNNNNNNGC 6 cut(s) 123, 163, 258, 321, 324, 333
NaeI GCCGGC 1 cut(s) 124
NarI GGCGCC 1 cut(s) 264
NciI CCSGG 1 cut(s) 288
NdeII GATC 1 cut(s) 180
NgoMIV GCCGGC 1 cut(s) 122
NlaIV GGNNCC 2 cut(s) 118, 265
NruI TCGCGA 1 cut(s) 256
PdiI GCCGGC 1 cut(s) 124
PkrI GCNGC 8 cut(s) 64, 82, 85, 165, 263, 317, 326, 338
PleI GAGTC 1 cut(s) 167
PluTI GGCGCC 1 cut(s) 267
PpsI GAGTC 1 cut(s) 167
Psp124BI GAGCTC 1 cut(s) 295
Psp6I CCWGG 2 cut(s) 57, 206
PspFI CCCAGC 1 cut(s) 111
PspGI CCWGG 2 cut(s) 57, 206
PspN4I GGNNCC 2 cut(s) 118, 265
PspPI GGNCC 1 cut(s) 285
PstI CTGCAG 2 cut(s) 168, 341
PvuII CAGCTG 1 cut(s) 111
RruI TCGCGA 1 cut(s) 256
RsaI GTAC 2 cut(s) 212, 272
RsaNI GTAC 2 cut(s) 211, 271
SacI GAGCTC 1 cut(s) 295
SatI GCNGC 8 cut(s) 63, 81, 84, 164, 262, 316, 325, 337
Sau3AI GATC 1 cut(s) 180
Sau96I GGNCC 1 cut(s) 285
SchI GAGTC 1 cut(s) 167
ScrFI CCNGG 3 cut(s) 59, 208, 288
SduI GDGCHC 2 cut(s) 119, 295
SetI ASST 8 cut(s) 11, 113, 142, 159, 165, 212, 221, 295
SexAI ACCWGGT 1 cut(s) 206
SfaNI GCATC 2 cut(s) 201, 336
SfcI CTRYAG 3 cut(s) 164, 319, 337
SfoI GGCGCC 1 cut(s) 265
SgrAI CRCCGGYG 1 cut(s) 122
Sse9I AATT 1 cut(s) 277
SsiI CCGC 9 cut(s) 63, 81, 84, 96, 120, 126, 261, 315, 325
SspDI GGCGCC 1 cut(s) 263
SstI GAGCTC 1 cut(s) 295
StyD4I CCNGG 3 cut(s) 57, 206, 286
TaaI ACNGT 2 cut(s) 224, 244
TaqI TCGA 1 cut(s) 102
TasI AATT 1 cut(s) 277
TauI GCSGC 6 cut(s) 65, 83, 86, 264, 318, 327
TscAI CASTG 2 cut(s) 27, 247
TseI GCWGC 2 cut(s) 163, 336
TspDTI ATGAA 1 cut(s) 28
TspRI CASTG 2 cut(s) 27, 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.