FvH4_4g25640

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0013376
Physical Location & Seq
Forward (+)
27368220 .. 27369213
994 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g25640.t1

Sequence Viewer

Length: 402 bp
ATGCAGGGTTCTGAAGTGGAGAAGATTGTTGTCATGGATGATCCTCAGAACGAGATCATCACCAGCGACAACGATGTGATCATGAAGGTGGATATTCCGGTGGAGATTCCGGTTGATGATCCGCCGAGTACTCCACGTGAAATGGCATGTGGTCATCACCATGGGCTTACAAGGCAGCAGAGCCTGTCTAGGTCGACGATGGGTGCTAACTGCCTTTGCTCCCCGACCACTCACGTAGGTTCATTCCGGTGCCGGCTCCACCGTTCTCCCAGCCTCAATAAGTCTTCGAGCTTCAAAACGCCGAGCCTCCAGAGGACAAAGAGCAGCCTGGACTATGGATCATCATCGCCGCGCCGAGGAGGAAGAATGGAGGCCACATCCAAGCCTGAGCAGGCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

134

Amino Acids

14.55

Weight (kDa)

7.74

Isoelectric Point (pI)

62.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013376)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G24577 AT1G67910 AT1G67910
fragaria_vesca FvH4_4g25640 FvH4_4g25640
malus_domestica MD13G1100900.v1.1 MD16G1101300.v1.1
prunus_persica Prupe.1G245600_v2.0.a1
pyrus_communis pycom16g08580
rosa_chinensis RchiOBHm_Chr4g0433081
rosa_laevigata RLG00000006788
rosa_roxburghii Rroxscaffold_5G00374560
rosa_rugosa Rorug04G0265100 Rorug04G0265200.1
rosa_samantha Rh4BG327300 Rh4CG342700 Rh4DG322400
rosa_wichuraiana Rw4G027740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 249
AccI GTMKAC 1 cut(s) 194
AccII CGCG 1 cut(s) 352
AciI CCGC 2 cut(s) 122, 350
AclWI GGATC 3 cut(s) 35, 113, 346
AcuI CTGAAG 1 cut(s) 33
AcvI CACGTG 1 cut(s) 137
AfaI GTAC 1 cut(s) 130
AfiI CCNNNNNNNGG 1 cut(s) 356
AgsI TTSAA 1 cut(s) 295
AjnI CCWGG 1 cut(s) 327
AluBI AGCT 2 cut(s) 291, 399
AluI AGCT 2 cut(s) 291, 399
AlwI GGATC 3 cut(s) 35, 113, 346
AlwNI CAGNNNCTG 1 cut(s) 184
AoxI GGCC 1 cut(s) 372
ApeKI GCWGC 2 cut(s) 175, 324
AspLEI GCGC 1 cut(s) 354
AsuHPI GGTGA 2 cut(s) 52, 149
AsuNHI GCTAGC 1 cut(s) 395
BanI GGYRCC 1 cut(s) 249
BbrPI CACGTG 1 cut(s) 137
BbsI GAAGAC 1 cut(s) 276
BbvI GCAGC 2 cut(s) 187, 336
BccI CCATC 1 cut(s) 193
BciT130I CCWGG 1 cut(s) 329
BclI TGATCA 1 cut(s) 78
BfaI CTAG 3 cut(s) 189, 396, 400
BisI GCNGC 3 cut(s) 176, 325, 350
BlsI GCNGC 3 cut(s) 177, 326, 351
BmcAI AGTACT 1 cut(s) 130
Bme1390I CCNGG 1 cut(s) 329
BmiI GGNNCC 2 cut(s) 251, 257
BmrFI CCNGG 1 cut(s) 329
BmtI GCTAGC 1 cut(s) 399
BpiI GAAGAC 1 cut(s) 276
BpmI CTGGAG 1 cut(s) 293
Bpu10I CCTNAGC 1 cut(s) 387
BsaAI YACGTR 2 cut(s) 137, 235
BsaBI GATNNNNATC 1 cut(s) 343
BsaJI CCNNGG 2 cut(s) 160, 355
BsaWI WCCGGW 3 cut(s) 97, 109, 246
Bsc4I CCNNNNNNNGG 1 cut(s) 356
Bse118I RCCGGY 1 cut(s) 252
Bse8I GATNNNNATC 1 cut(s) 343
BseBI CCWGG 1 cut(s) 329
BseDI CCNNGG 2 cut(s) 160, 355
BseGI GGATG 2 cut(s) 43, 377
BseJI GATNNNNATC 1 cut(s) 343
BseLI CCNNNNNNNGG 1 cut(s) 356
BseMII CTCAG 2 cut(s) 59, 378
BseRI GAGGAG 1 cut(s) 372
BseXI GCAGC 2 cut(s) 187, 336
BseYI CCCAGC 1 cut(s) 269
Bsh1236I CGCG 1 cut(s) 352
BshFI GGCC 1 cut(s) 374
BshNI GGYRCC 1 cut(s) 249
BsiSI CCGG 4 cut(s) 98, 110, 247, 253
BslI CCNNNNNNNGG 1 cut(s) 356
BsnI GGCC 1 cut(s) 374
Bsp143I GATC 5 cut(s) 40, 54, 78, 118, 338
Bsp19I CCATGG 1 cut(s) 160
BspACI CCGC 2 cut(s) 122, 350
BspANI GGCC 1 cut(s) 374
BspCNI CTCAG 2 cut(s) 58, 379
BspFNI CGCG 1 cut(s) 352
BspHI TCATGA 1 cut(s) 81
BspLI GGNNCC 2 cut(s) 251, 257
BspOI GCTAGC 1 cut(s) 399
BspPI GGATC 3 cut(s) 35, 113, 346
BspT107I GGYRCC 1 cut(s) 249
BsrFI RCCGGY 1 cut(s) 252
BssAI RCCGGY 1 cut(s) 252
BssECI CCNNGG 2 cut(s) 160, 355
BssMI GATC 5 cut(s) 40, 54, 78, 118, 338
BssT1I CCWWGG 1 cut(s) 160
Bst2UI CCWGG 1 cut(s) 329
Bst4CI ACNGT 1 cut(s) 263
BstBAI YACGTR 2 cut(s) 137, 235
BstC8I GCNNGC 3 cut(s) 254, 393, 397
BstDEI CTNAG 2 cut(s) 45, 387
BstDSI CCRYGG 1 cut(s) 160
BstF5I GGATG 2 cut(s) 43, 377
BstFNI CGCG 1 cut(s) 352
BstHHI GCGC 1 cut(s) 354
BstKTI GATC 5 cut(s) 43, 57, 81, 121, 341
BstMBI GATC 5 cut(s) 40, 54, 78, 118, 338
BstMWI GCNNNNNNNGC 1 cut(s) 172
BstNI CCWGG 1 cut(s) 329
BstNSI RCATGY 1 cut(s) 150
BstSCI CCNGG 1 cut(s) 327
BstUI CGCG 1 cut(s) 352
BstV1I GCAGC 2 cut(s) 187, 336
BstV2I GAAGAC 1 cut(s) 276
BsuRI GGCC 1 cut(s) 374
BtgI CCRYGG 1 cut(s) 160
BtgZI GCGATG 1 cut(s) 330
BtsCI GGATG 2 cut(s) 43, 377
Cac8I GCNNGC 3 cut(s) 254, 393, 397
CaiI CAGNNNCTG 1 cut(s) 184
CciI TCATGA 1 cut(s) 81
CfoI GCGC 1 cut(s) 354
Cfr10I RCCGGY 1 cut(s) 252
Csp6I GTAC 1 cut(s) 129
CviAII CATG 4 cut(s) 34, 82, 147, 161
CviQI GTAC 1 cut(s) 129
DdeI CTNAG 2 cut(s) 45, 387
DpnI GATC 5 cut(s) 42, 56, 80, 120, 340
DpnII GATC 5 cut(s) 40, 54, 78, 118, 338
EciI GGCGGA 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 160
Eco57I CTGAAG 1 cut(s) 33
Eco72I CACGTG 1 cut(s) 137
EcoRII CCWGG 1 cut(s) 327
EcoT14I CCWWGG 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 160
FaeI CATG 4 cut(s) 37, 85, 150, 164
FaiI YATR 5 cut(s) 35, 83, 148, 162, 336
FatI CATG 4 cut(s) 33, 81, 146, 160
FbaI TGATCA 1 cut(s) 78
FblI GTMKAC 1 cut(s) 194
Fnu4HI GCNGC 3 cut(s) 176, 325, 350
FokI GGATG 2 cut(s) 50, 364
Fsp4HI GCNGC 3 cut(s) 176, 325, 350
FspBI CTAG 3 cut(s) 189, 396, 400
GlaI GCGC 1 cut(s) 353
GluI GCNGC 3 cut(s) 176, 325, 350
GsaI CCCAGC 1 cut(s) 273
GsuI CTGGAG 1 cut(s) 293
HaeIII GGCC 1 cut(s) 374
HapII CCGG 4 cut(s) 98, 110, 247, 253
HhaI GCGC 1 cut(s) 354
Hin1II CATG 4 cut(s) 37, 85, 150, 164
Hin6I GCGC 1 cut(s) 352
HinP1I GCGC 1 cut(s) 352
HincII GTYRAC 1 cut(s) 195
HindII GTYRAC 1 cut(s) 195
HinfI GANTC 1 cut(s) 106
HpaII CCGG 4 cut(s) 98, 110, 247, 253
HphI GGTGA 2 cut(s) 52, 149
Hpy166II GTNNAC 1 cut(s) 195
Hpy188I TCNGA 2 cut(s) 13, 48
Hpy188III TCNNGA 2 cut(s) 82, 310
Hpy8I GTNNAC 1 cut(s) 195
Hpy99I CGWCG 1 cut(s) 199
HpyAV CCTTC 1 cut(s) 79
HpyCH4III ACNGT 1 cut(s) 263
HpyCH4IV ACGT 2 cut(s) 136, 234
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 1 cut(s) 172
HpyF3I CTNAG 2 cut(s) 45, 387
HpySE526I ACGT 2 cut(s) 136, 234
Hsp92II CATG 4 cut(s) 37, 85, 150, 164
HspAI GCGC 1 cut(s) 352
KroI GCCGGC 1 cut(s) 252
KroNI GCCGGC 1 cut(s) 254
Ksp22I TGATCA 1 cut(s) 78
Kzo9I GATC 5 cut(s) 40, 54, 78, 118, 338
LmnI GCTCC 2 cut(s) 224, 261
Lsp1109I GCAGC 2 cut(s) 187, 336
MaeI CTAG 3 cut(s) 189, 396, 400
MaeII ACGT 2 cut(s) 136, 234
MalI GATC 5 cut(s) 42, 56, 80, 120, 340
MboI GATC 5 cut(s) 40, 54, 78, 118, 338
MboII GAAGA 3 cut(s) 34, 276, 375
MnlI CCTC 7 cut(s) 54, 284, 306, 317, 350, 353, 364
MroNI GCCGGC 1 cut(s) 252
MslI CAYNNNNRTG 3 cut(s) 86, 159, 247
MspI CCGG 4 cut(s) 98, 110, 247, 253
MspR9I CCNGG 1 cut(s) 329
MvaI CCWGG 1 cut(s) 329
MvnI CGCG 1 cut(s) 352
MwoI GCNNNNNNNGC 1 cut(s) 172
NaeI GCCGGC 1 cut(s) 254
NcoI CCATGG 1 cut(s) 160
NdeII GATC 5 cut(s) 40, 54, 78, 118, 338
NgoMIV GCCGGC 1 cut(s) 252
NheI GCTAGC 1 cut(s) 395
NlaIII CATG 4 cut(s) 37, 85, 150, 164
NlaIV GGNNCC 2 cut(s) 251, 257
NmeAIII GCCGAG 3 cut(s) 150, 327, 380
NspI RCATGY 1 cut(s) 150
PagI TCATGA 1 cut(s) 81
PdiI GCCGGC 1 cut(s) 254
PfeI GAWTC 1 cut(s) 106
PkrI GCNGC 3 cut(s) 177, 326, 351
PmaCI CACGTG 1 cut(s) 137
PmlI CACGTG 1 cut(s) 137
Ppu21I YACGTR 2 cut(s) 137, 235
Psp6I CCWGG 1 cut(s) 327
PspCI CACGTG 1 cut(s) 137
PspFI CCCAGC 1 cut(s) 269
PspGI CCWGG 1 cut(s) 327
PspN4I GGNNCC 2 cut(s) 251, 257
PstNI CAGNNNCTG 1 cut(s) 184
RsaI GTAC 1 cut(s) 130
RsaNI GTAC 1 cut(s) 129
RseI CAYNNNNRTG 3 cut(s) 86, 159, 247
SalI GTCGAC 1 cut(s) 193
SatI GCNGC 3 cut(s) 176, 325, 350
Sau3AI GATC 5 cut(s) 40, 54, 78, 118, 338
ScaI AGTACT 1 cut(s) 130
ScrFI CCNGG 1 cut(s) 329
SetI ASST 7 cut(s) 90, 139, 194, 237, 241, 293, 401
SmiMI CAYNNNNRTG 3 cut(s) 86, 159, 247
SsiI CCGC 2 cut(s) 122, 350
SspMI CTAG 3 cut(s) 189, 396, 400
StyD4I CCNGG 1 cut(s) 327
StyI CCWWGG 1 cut(s) 160
TaaI ACNGT 1 cut(s) 263
TaiI ACGT 2 cut(s) 139, 237
TaqI TCGA 2 cut(s) 194, 287
TatI WGTACW 1 cut(s) 128
TauI GCSGC 1 cut(s) 352
TfiI GAWTC 1 cut(s) 106
TseI GCWGC 2 cut(s) 175, 324
TspDTI ATGAA 2 cut(s) 98, 231
XceI RCATGY 1 cut(s) 150
XmiI GTMKAC 1 cut(s) 194
XspI CTAG 3 cut(s) 189, 396, 400
ZrmI AGTACT 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.