FvH4_4g27390

Zinc finger protein CONSTANS-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0012185
Physical Location & Seq
Reverse (-)
28409309 .. 28411538
2230 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27390.t1

Sequence Viewer

Length: 1353 bp
ATGATATCAAACAAGAAAGCATCTGCTTCTGCTTCTGCGGCAACAGCGAACGTCGTTGGAGGGAAGACGGCGAGAGCGTGTGACAGCTGTATAAAGAAGAGGGCTAGATGGTACTGTGCTGCTGACGATGCTTTTCTATGTCAAGCGTGCGACTCCTCCGTGCACTCAGCCAACCAGTTGGCTAGGAGACATGAACGCGTCCGGTTGAAAACTGCGTCTTCGATCAAGTCCACTACCAACTCCGGGAGTTGTGACAAATCCGAAGTGCCATCATGGCACCGAGGGTTCACGAGGAAACCGCGGACACCGCGGGGGCAAGGCAAGTCTGCGGCTCCTCGGAACCGATTTCCTCTTGTTCCGGAAGTCGGAGCGGACGACCTGTCGTATGAGGAGAACGAGGAGGAGCAGCTTCTATATAGAGTTCCGATATACGATCCCTTTGTTGCGGAGCTCTGCACTTCTCCTAATTCCAACGCTGTTTCCAACGATCTCAATGGAAACGAATCCAAAGCGGTTTCGATGCCTAATTACTATGGTGTGGGAAATAGAAACTCACCTTCATCACATGGATTTGATCTTCCGAGTGATATGGATCTGGCAGAGTTTGCAGCTGATGTTGATAGCTTATTGGGGAGAGGGCTCGATGATCATGAGTGTTTTGGTATGGAAGGGTTGGGATTAACGGATTGTCATGAAAAGGAATCCACGACTACGGAGGAGGGTACTACCGAAAGAGTGAAGGTTGAAGATGAGGAAGATGCTTATATGGCGTGCCAAGCTGAGACGGAGATGGATTTGATGAGGGAGCCTTTTGTGTTGAACTTTGAAGACTACGACGACTCGCCTGCCTCCTGCGCCGAAGATGAAGAGGAGGAGGAGGAGGAGGATCATAAACTGGGAGTGAGAATGAGTCATGGTGAGACGAAGAGTAGTTGTCAAGAGCATAAAAGTTGCAAGAAGAGGAAGATATTTTTGAGGCTGGATTACGATGAGGTGATTACCAAATGGGATAGCCAAAGTAATGGATCTCCGTTTGCATTTGGAGATCGGCCCGATATTGATTACGACTGCCTACCTGACTTCATGGGCACTGCAAGTGGAGCCGAGTTACTTCATTACCCCTACGGAGATTTGAACAGAGTAATTGGGTTACATCCGACAATGACCGATGGAGGGAGAGAGGCGAGGGTGTCAAGGTACAGAGAAAAGAGGAGGACGAGGCTGTTCTCGAAGAAAATAAGATACGAAGTCAGGAAACTGAATGCTGAGAAGAGGCCGAGAATGAAAGGTAGATTTGTAAAGAGAGCTTCATTTGCAGCTACAGGATCTACATTTCCTATGCTTACTAAATGA

Protein Analysis

451

Amino Acids

50.33

Weight (kDa)

5.56

Isoelectric Point (pI)

58.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-B_box PF00643 24 - 67 2.5e-07 B-box zinc finger
CCT PF06203 393 - 435 6.1e-17 CCT motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012185)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25440 AT1G68520
fragaria_vesca FvH4_4g27390
malus_domestica MD13G1042200.v1.1 MD16G1043000.v1.1
prunus_persica Prupe.1G310900_v2.0.a1
pyrus_communis pycom13g03650 pycom16g03710
rosa_chinensis RchiOBHm_Chr4g0435191
rosa_laevigata RLG00000006610
rosa_multiflora Rmu_ssc0000339.1_g000030
rosa_roxburghii Rroxscaffold_5G00376410
rosa_rugosa Rorug04G0282900 Rorug04G0283000
rosa_samantha Rh4AG338700 Rh4BG346800 Rh4CG361400 Rh4DG341200
rosa_wichuraiana Rw4G029540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 276
AccBSI CCGCTC 1 cut(s) 371
AccII CGCG 3 cut(s) 198, 301, 310
AccIII TCCGGA 1 cut(s) 358
AciI CCGC 9 cut(s) 38, 299, 301, 308, 310, 329, 371, 446, 512
AclWI GGATC 5 cut(s) 428, 600, 894, 1033, 1333
AfaI GTAC 3 cut(s) 113, 724, 1199
AfiI CCNNNNNNNGG 3 cut(s) 243, 365, 1173
AflIII ACRYGT 1 cut(s) 196
AgsI TTSAA 5 cut(s) 208, 746, 820, 827, 1135
AhdI GACNNNNNGTC 1 cut(s) 379
AluBI AGCT 8 cut(s) 87, 409, 451, 611, 624, 779, 1307, 1319
AluI AGCT 8 cut(s) 87, 409, 451, 611, 624, 779, 1307, 1319
Alw21I GWGCWC 2 cut(s) 165, 453
Alw26I GTCTC 3 cut(s) 181, 776, 914
Alw44I GTGCAC 1 cut(s) 161
AlwI GGATC 5 cut(s) 428, 600, 894, 1033, 1333
Aor13HI TCCGGA 1 cut(s) 358
AoxI GGCC 2 cut(s) 1049, 1274
ApaLI GTGCAC 1 cut(s) 161
ApeKI GCWGC 4 cut(s) 119, 406, 608, 1316
AspLEI GCGC 1 cut(s) 857
AspS9I GGNCC 1 cut(s) 1050
AsuC2I CCSGG 1 cut(s) 244
AsuHPI GGTGA 3 cut(s) 546, 929, 1006
BaeGI GKGCMC 2 cut(s) 165, 1091
BanI GGYRCC 1 cut(s) 276
BanII GRGCYC 2 cut(s) 453, 642
BauI CACGAG 1 cut(s) 289
BbsI GAAGAC 3 cut(s) 71, 210, 834
Bbv12I GWGCWC 2 cut(s) 165, 453
BbvI GCAGC 4 cut(s) 106, 418, 620, 1328
BccI CCATC 4 cut(s) 102, 277, 784, 1163
BceAI ACGGC 1 cut(s) 84
BcgI CGANNNNNNTGC 2 cut(s) 827, 861
BclI TGATCA 1 cut(s) 646
BcnI CCSGG 1 cut(s) 244
BcoDI GTCTC 3 cut(s) 181, 776, 914
BfaI CTAG 2 cut(s) 105, 183
BfmI CTRYAG 1 cut(s) 1320
BglI GCCNNNNNGGC 1 cut(s) 274
BisI GCNGC 6 cut(s) 39, 120, 330, 407, 609, 1317
BlsI GCNGC 6 cut(s) 40, 121, 331, 408, 610, 1318
Bme1390I CCNGG 1 cut(s) 244
BmeRI GACNNNNNGTC 1 cut(s) 379
BmgT120I GGNCC 1 cut(s) 1050
BmiI GGNNCC 5 cut(s) 278, 333, 341, 807, 1102
BmrFI CCNGG 1 cut(s) 244
BmrI ACTGGG 1 cut(s) 905
BmsI GCATC 4 cut(s) 29, 118, 510, 748
BmuI ACTGGG 1 cut(s) 905
BpiI GAAGAC 3 cut(s) 71, 210, 834
BpuMI CCSGG 1 cut(s) 244
BsaBI GATNNNNATC 1 cut(s) 591
BsaJI CCNNGG 4 cut(s) 280, 299, 308, 335
BsaWI WCCGGW 2 cut(s) 201, 358
Bsc4I CCNNNNNNNGG 3 cut(s) 243, 365, 1173
Bse1I ACTGG 2 cut(s) 175, 900
Bse8I GATNNNNATC 1 cut(s) 591
BseAI TCCGGA 1 cut(s) 358
BseDI CCNNGG 4 cut(s) 280, 299, 308, 335
BseGI GGATG 1 cut(s) 1153
BseJI GATNNNNATC 1 cut(s) 591
BseLI CCNNNNNNNGG 3 cut(s) 243, 365, 1173
BseMII CTCAG 3 cut(s) 180, 771, 1257
BseNI ACTGG 2 cut(s) 175, 900
BseSI GKGCMC 2 cut(s) 165, 1091
BseXI GCAGC 4 cut(s) 106, 418, 620, 1328
BsgI GTGCAG 1 cut(s) 439
Bsh1236I CGCG 3 cut(s) 198, 301, 310
BshFI GGCC 2 cut(s) 1051, 1276
BshNI GGYRCC 1 cut(s) 276
BsiHKAI GWGCWC 2 cut(s) 165, 453
BsiSI CCGG 3 cut(s) 202, 243, 359
BslI CCNNNNNNNGG 3 cut(s) 243, 365, 1173
BsmAI GTCTC 3 cut(s) 181, 776, 914
BsmBI CGTCTC 2 cut(s) 776, 914
BsmI GAATGC 1 cut(s) 1267
BsnI GGCC 2 cut(s) 1051, 1276
Bsp1286I GDGCHC 4 cut(s) 165, 453, 642, 1091
Bsp13I TCCGGA 1 cut(s) 358
BspACI CCGC 9 cut(s) 38, 299, 301, 308, 310, 329, 371, 446, 512
BspANI GGCC 2 cut(s) 1051, 1276
BspCNI CTCAG 3 cut(s) 179, 772, 1258
BspEI TCCGGA 1 cut(s) 358
BspFNI CGCG 3 cut(s) 198, 301, 310
BspHI TCATGA 2 cut(s) 649, 691
BspLI GGNNCC 5 cut(s) 278, 333, 341, 807, 1102
BspPI GGATC 5 cut(s) 428, 600, 894, 1033, 1333
BspT107I GGYRCC 1 cut(s) 276
BsrBI CCGCTC 1 cut(s) 371
BsrI ACTGG 2 cut(s) 175, 900
BssECI CCNNGG 4 cut(s) 280, 299, 308, 335
BssSI CACGAG 1 cut(s) 289
Bst2BI CACGAG 1 cut(s) 289
Bst4CI ACNGT 1 cut(s) 116
Bst6I CTCTTC 5 cut(s) 92, 861, 920, 953, 1265
BstAPI GCANNNNNTGC 1 cut(s) 605
BstC8I GCNNGC 3 cut(s) 148, 772, 846
BstDEI CTNAG 3 cut(s) 166, 780, 1266
BstDSI CCRYGG 2 cut(s) 299, 308
BstF5I GGATG 1 cut(s) 1153
BstFNI CGCG 3 cut(s) 198, 301, 310
BstHHI GCGC 1 cut(s) 857
BstMAI GTCTC 3 cut(s) 181, 776, 914
BstSCI CCNGG 1 cut(s) 242
BstSFI CTRYAG 1 cut(s) 1320
BstSLI GKGCMC 2 cut(s) 165, 1091
BstUI CGCG 3 cut(s) 198, 301, 310
BstV1I GCAGC 4 cut(s) 106, 418, 620, 1328
BstV2I GAAGAC 3 cut(s) 71, 210, 834
BstX2I RGATCY 3 cut(s) 592, 1025, 1325
BstXI CCANNNNNNTGG 2 cut(s) 178, 1022
BstYI RGATCY 3 cut(s) 592, 1025, 1325
BsuRI GGCC 2 cut(s) 1051, 1276
BtgI CCRYGG 2 cut(s) 299, 308
BtsCI GGATG 1 cut(s) 1153
BtsI GCAGTG 1 cut(s) 1089
BtsIMutI CAGTG 1 cut(s) 1089
Cac8I GCNNGC 3 cut(s) 148, 772, 846
CciI TCATGA 2 cut(s) 649, 691
CfoI GCGC 1 cut(s) 857
Cfr13I GGNCC 1 cut(s) 1050
Cfr42I CCGCGG 2 cut(s) 302, 311
CseI GACGC 2 cut(s) 187, 204
Csp6I GTAC 3 cut(s) 112, 723, 1198
CviAII CATG 7 cut(s) 191, 273, 566, 650, 692, 914, 1084
CviQI GTAC 3 cut(s) 112, 723, 1198
DdeI CTNAG 3 cut(s) 166, 780, 1266
DriI GACNNNNNGTC 1 cut(s) 379
Eam1104I CTCTTC 5 cut(s) 92, 861, 920, 953, 1265
Eam1105I GACNNNNNGTC 1 cut(s) 379
EarI CTCTTC 5 cut(s) 92, 861, 920, 953, 1265
Ecl136II GAGCTC 1 cut(s) 451
Eco24I GRGCYC 2 cut(s) 453, 642
Eco32I GATATC 1 cut(s) 6
Eco53kI GAGCTC 1 cut(s) 451
EcoICRI GAGCTC 1 cut(s) 451
EcoRV GATATC 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 453, 642
Esp3I CGTCTC 2 cut(s) 776, 914
FaeI CATG 7 cut(s) 194, 276, 569, 653, 695, 917, 1087
FatI CATG 7 cut(s) 190, 272, 565, 649, 691, 913, 1083
FauI CCCGC 1 cut(s) 303
FbaI TGATCA 1 cut(s) 646
Fnu4HI GCNGC 6 cut(s) 39, 120, 330, 407, 609, 1317
FokI GGATG 1 cut(s) 1140
FriOI GRGCYC 2 cut(s) 453, 642
Fsp4HI GCNGC 6 cut(s) 39, 120, 330, 407, 609, 1317
FspBI CTAG 2 cut(s) 105, 183
GlaI GCGC 1 cut(s) 856
GluI GCNGC 6 cut(s) 39, 120, 330, 407, 609, 1317
HaeIII GGCC 2 cut(s) 1051, 1276
HapII CCGG 3 cut(s) 202, 243, 359
HgaI GACGC 2 cut(s) 187, 204
HhaI GCGC 1 cut(s) 857
Hin1II CATG 7 cut(s) 194, 276, 569, 653, 695, 917, 1087
Hin6I GCGC 1 cut(s) 855
HinP1I GCGC 1 cut(s) 855
HinfI GANTC 5 cut(s) 152, 503, 701, 839, 910
HpaII CCGG 3 cut(s) 202, 243, 359
HphI GGTGA 3 cut(s) 546, 929, 1006
Hpy166II GTNNAC 3 cut(s) 163, 231, 288
Hpy188I TCNGA 6 cut(s) 262, 339, 368, 426, 582, 1158
Hpy188III TCNNGA 7 cut(s) 289, 359, 650, 692, 938, 1228, 1252
Hpy8I GTNNAC 3 cut(s) 163, 231, 288
Hpy99I CGWCG 2 cut(s) 56, 839
HpyAV CCTTC 3 cut(s) 567, 662, 733
HpyCH4III ACNGT 1 cut(s) 116
HpyCH4IV ACGT 1 cut(s) 51
HpyCH4V TGCA 7 cut(s) 163, 456, 608, 954, 1037, 1094, 1316
HpyF3I CTNAG 3 cut(s) 166, 780, 1266
HpySE526I ACGT 1 cut(s) 51
Hsp92II CATG 7 cut(s) 194, 276, 569, 653, 695, 917, 1087
HspAI GCGC 1 cut(s) 855
Kpn2I TCCGGA 1 cut(s) 358
Ksp22I TGATCA 1 cut(s) 646
KspI CCGCGG 2 cut(s) 302, 311
LmnI GCTCC 6 cut(s) 337, 368, 403, 448, 805, 1100
Lsp1109I GCAGC 4 cut(s) 106, 418, 620, 1328
LweI GCATC 4 cut(s) 29, 118, 510, 748
MaeI CTAG 2 cut(s) 105, 183
MaeII ACGT 1 cut(s) 51
MaeIII GTNAC 4 cut(s) 80, 251, 1107, 1149
MbiI CCGCTC 1 cut(s) 371
MflI RGATCY 3 cut(s) 592, 1025, 1325
MhlI GDGCHC 4 cut(s) 165, 453, 642, 1091
MluCI AATT 3 cut(s) 466, 526, 1143
MluI ACGCGT 1 cut(s) 196
MlyI GAGTC 3 cut(s) 146, 833, 919
MmeI TCCRAC 5 cut(s) 37, 346, 495, 507, 1181
MroI TCCGGA 1 cut(s) 358
MseI TTAA 1 cut(s) 680
MspA1I CMGCKG 4 cut(s) 87, 301, 310, 611
MspI CCGG 3 cut(s) 202, 243, 359
MspR9I CCNGG 1 cut(s) 244
Mva1269I GAATGC 1 cut(s) 1267
MvnI CGCG 3 cut(s) 198, 301, 310
NciI CCSGG 1 cut(s) 244
NlaIII CATG 7 cut(s) 194, 276, 569, 653, 695, 917, 1087
NlaIV GGNNCC 5 cut(s) 278, 333, 341, 807, 1102
NmeAIII GCCGAG 2 cut(s) 1129, 1302
NmuCI GTSAC 2 cut(s) 80, 251
PagI TCATGA 2 cut(s) 649, 691
PcsI WCGNNNNNNNCGW 2 cut(s) 74, 372
PctI GAATGC 1 cut(s) 1267
PfeI GAWTC 2 cut(s) 503, 701
PfoI TCCNGGA 1 cut(s) 242
PkrI GCNGC 6 cut(s) 40, 121, 331, 408, 610, 1318
PleI GAGTC 3 cut(s) 146, 833, 918
PpsI GAGTC 3 cut(s) 146, 833, 918
Psp124BI GAGCTC 1 cut(s) 453
PspN4I GGNNCC 5 cut(s) 278, 333, 341, 807, 1102
PspPI GGNCC 1 cut(s) 1050
PsuI RGATCY 3 cut(s) 592, 1025, 1325
PvuII CAGCTG 2 cut(s) 87, 611
RsaI GTAC 3 cut(s) 113, 724, 1199
RsaNI GTAC 3 cut(s) 112, 723, 1198
SacI GAGCTC 1 cut(s) 453
SacII CCGCGG 2 cut(s) 302, 311
SaqAI TTAA 1 cut(s) 680
SatI GCNGC 6 cut(s) 39, 120, 330, 407, 609, 1317
Sau96I GGNCC 1 cut(s) 1050
SchI GAGTC 3 cut(s) 146, 833, 919
ScrFI CCNGG 1 cut(s) 244
SduI GDGCHC 4 cut(s) 165, 453, 642, 1091
SfaNI GCATC 4 cut(s) 29, 118, 510, 748
SfcI CTRYAG 1 cut(s) 1320
Sfr303I CCGCGG 2 cut(s) 302, 311
SgrBI CCGCGG 2 cut(s) 302, 311
Sse9I AATT 3 cut(s) 466, 526, 1143
SsiI CCGC 9 cut(s) 38, 299, 301, 308, 310, 329, 371, 446, 512
SspMI CTAG 2 cut(s) 105, 183
SstI GAGCTC 1 cut(s) 453
StyD4I CCNGG 1 cut(s) 242
TaaI ACNGT 1 cut(s) 116
TaiI ACGT 1 cut(s) 54
TaqI TCGA 4 cut(s) 221, 518, 642, 1229
TaqII GACCGA 1 cut(s) 1181
TasI AATT 3 cut(s) 466, 526, 1143
TauI GCSGC 2 cut(s) 41, 332
TfiI GAWTC 2 cut(s) 503, 701
Tru1I TTAA 1 cut(s) 680
Tru9I TTAA 1 cut(s) 680
TscAI CASTG 1 cut(s) 1096
TseFI GTSAC 2 cut(s) 80, 251
TseI GCWGC 4 cut(s) 119, 406, 608, 1316
Tsp45I GTSAC 2 cut(s) 80, 251
TspDTI ATGAA 8 cut(s) 207, 549, 708, 879, 1072, 1103, 1298, 1299
TspGWI ACGGA 6 cut(s) 148, 698, 728, 800, 1020, 1140
TspRI CASTG 1 cut(s) 1096
VneI GTGCAC 1 cut(s) 161
XspI CTAG 2 cut(s) 105, 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.