pycom16g03710

Zinc finger protein CONSTANS-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0012185
Physical Location & Seq
Reverse (-)
2304156 .. 2304866
711 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g03710.1

Sequence Viewer

Length: 711 bp
ATGAATTCTAGCAAGAAAGTGGCACATGCCGTCGGCGGTAAAACTGCCCGAGCGTGTGACAGCTGCATAAAAAAGAGAGCTCGTTGGTACTGCGCTGCTGACGACGCTTTCTTATGCGAAGCCTGCGACTCCGACGTGCACTCCGCCAACCAATTGGCCCGTCGGCACGAAAGGGTTCTCTTGAAGACGGCGTCGTCTCTCAAACCTAGCAACAAAGATGATAGAACGACCGCTGTTTCGAAGACCTCGGCGGCACCGTCTTGGCACCGCGGGTTCACGCGGAAGCCGCGAACGCCGCGGGGCGGGAAGTCCATGACACATCATGGAAAATCCAAGGAAGGAATCCGGAACCTGTTTCCGCAGGTTCCGGAAGTTGGGGGAGACGATAATAACTCGTACGATGAAGAAAATGAGGAAGACCAGCAGCTGCTTTATAGGGTTCCGATATTCGATCCGTCTGTTGCGGAGCTGTGCAGTAACTCTGGTAACTCTAGTGAAGATGCAGCTGCGAATTACGAAGCTAATCTTAGTACTGTTTCTGATAATGTTGGAAGCCAATCCAAAGCAGTTTCGTCGTTGCCGAATTGTAACGGCCATGATGGGTTTCTGCTTCCGAGCGACATGGATCGATTGCGGAGTTCGCGGCTGACGTGGATAGTTTGCTGGGGAGGGGCCTCAATGACGATCAGTGTATTGGGATGGAAGGGTTAG

Protein Analysis

237

Amino Acids

25.68

Weight (kDa)

8.4

Isoelectric Point (pI)

46.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-B_box PF00643 16 - 60 3e-07 B-box zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012185)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25440 AT1G68520
fragaria_vesca FvH4_4g27390
malus_domestica MD13G1042200.v1.1 MD16G1043000.v1.1
prunus_persica Prupe.1G310900_v2.0.a1
pyrus_communis pycom13g03650 pycom16g03710
rosa_chinensis RchiOBHm_Chr4g0435191
rosa_laevigata RLG00000006610
rosa_multiflora Rmu_ssc0000339.1_g000030
rosa_roxburghii Rroxscaffold_5G00376410
rosa_rugosa Rorug04G0282900 Rorug04G0283000
rosa_samantha Rh4AG338700 Rh4BG346800 Rh4CG361400 Rh4DG341200
rosa_wichuraiana Rw4G029540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 193
Acc36I ACCTGC 1 cut(s) 352
AccB1I GGYRCC 2 cut(s) 253, 264
AccII CGCG 5 cut(s) 270, 280, 289, 298, 643
AccIII TCCGGA 2 cut(s) 345, 367
AclWI GGATC 2 cut(s) 446, 633
AcoI YGGCCR 1 cut(s) 592
AcsI RAATTY 1 cut(s) 4
AcyI GRCGYC 1 cut(s) 191
AfaI GTAC 3 cut(s) 89, 398, 532
AfiI CCNNNNNNNGG 2 cut(s) 302, 374
AgsI TTSAA 1 cut(s) 184
AjiI CACGTC 2 cut(s) 136, 651
AluBI AGCT 6 cut(s) 63, 80, 427, 469, 506, 521
AluI AGCT 6 cut(s) 63, 80, 427, 469, 506, 521
Alw21I GWGCWC 2 cut(s) 82, 141
Alw26I GTCTC 2 cut(s) 201, 375
Alw44I GTGCAC 1 cut(s) 137
AlwI GGATC 2 cut(s) 446, 633
AlwNI CAGNNNCTG 1 cut(s) 427
Ama87I CYCGRG 1 cut(s) 48
Aor13HI TCCGGA 2 cut(s) 345, 367
AoxI GGCC 3 cut(s) 156, 592, 672
ApaLI GTGCAC 1 cut(s) 137
ApeKI GCWGC 6 cut(s) 63, 95, 424, 427, 503, 506
ApoI RAATTY 1 cut(s) 4
ArsI GACNNNNNNTTYG 2 cut(s) 220, 252
Asp700I GAANNNNTTC 1 cut(s) 174
AspLEI GCGC 1 cut(s) 95
AspS9I GGNCC 2 cut(s) 157, 672
AsuII TTCGAA 1 cut(s) 239
AvaI CYCGRG 1 cut(s) 48
BaeGI GKGCMC 1 cut(s) 141
BanI GGYRCC 2 cut(s) 253, 264
BanII GRGCYC 1 cut(s) 82
BbsI GAAGAC 3 cut(s) 191, 248, 423
Bbv12I GWGCWC 2 cut(s) 82, 141
BbvI GCAGC 6 cut(s) 50, 82, 414, 436, 493, 515
BccI CCATC 2 cut(s) 593, 693
BceAI ACGGC 3 cut(s) 14, 204, 607
BcgI CGANNNNNNTGC 2 cut(s) 555, 589
BcoDI GTCTC 2 cut(s) 201, 375
BfaI CTAG 3 cut(s) 9, 207, 492
BfuAI ACCTGC 1 cut(s) 352
BmcAI AGTACT 1 cut(s) 532
BmeT110I CYCGRG 1 cut(s) 48
BmgBI CACGTC 2 cut(s) 136, 651
BmgT120I GGNCC 2 cut(s) 157, 672
BmiI GGNNCC 6 cut(s) 255, 266, 350, 366, 441, 673
BmsI GCATC 1 cut(s) 490
BpiI GAAGAC 3 cut(s) 191, 248, 423
Bpu14I TTCGAA 1 cut(s) 239
Bsa29I ATCGAT 1 cut(s) 628
BsaHI GRCGYC 1 cut(s) 191
BsaJI CCNNGG 4 cut(s) 246, 268, 296, 333
BsaWI WCCGGW 2 cut(s) 345, 367
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 2 cut(s) 302, 374
BseAI TCCGGA 2 cut(s) 345, 367
BseCI ATCGAT 1 cut(s) 628
BseDI CCNNGG 4 cut(s) 246, 268, 296, 333
BseGI GGATG 1 cut(s) 704
BseLI CCNNNNNNNGG 2 cut(s) 302, 374
BseSI GKGCMC 1 cut(s) 141
BseXI GCAGC 6 cut(s) 50, 82, 414, 436, 493, 515
BseYI CCCAGC 1 cut(s) 663
BsgI GTGCAG 1 cut(s) 493
Bsh1236I CGCG 5 cut(s) 270, 280, 289, 298, 643
Bsh1285I CGRYCG 1 cut(s) 231
BshFI GGCC 3 cut(s) 158, 594, 674
BshNI GGYRCC 2 cut(s) 253, 264
BshVI ATCGAT 1 cut(s) 628
BsiEI CGRYCG 1 cut(s) 231
BsiHKAI GWGCWC 2 cut(s) 82, 141
BsiHKCI CYCGRG 1 cut(s) 48
BsiSI CCGG 2 cut(s) 346, 368
BsiWI CGTACG 1 cut(s) 396
BslI CCNNNNNNNGG 2 cut(s) 302, 374
BsmAI GTCTC 2 cut(s) 201, 375
BsmBI CGTCTC 2 cut(s) 201, 375
BsnI GGCC 3 cut(s) 158, 594, 674
BsoBI CYCGRG 1 cut(s) 48
Bsp119I TTCGAA 1 cut(s) 239
Bsp1286I GDGCHC 2 cut(s) 82, 141
Bsp13I TCCGGA 2 cut(s) 345, 367
Bsp143I GATC 3 cut(s) 451, 625, 684
BspANI GGCC 3 cut(s) 158, 594, 674
BspDI ATCGAT 1 cut(s) 628
BspEI TCCGGA 2 cut(s) 345, 367
BspFNI CGCG 5 cut(s) 270, 280, 289, 298, 643
BspLI GGNNCC 6 cut(s) 255, 266, 350, 366, 441, 673
BspMI ACCTGC 1 cut(s) 352
BspPI GGATC 2 cut(s) 446, 633
BspT104I TTCGAA 1 cut(s) 239
BspT107I GGYRCC 2 cut(s) 253, 264
BssECI CCNNGG 4 cut(s) 246, 268, 296, 333
BssMI GATC 3 cut(s) 451, 625, 684
BssNI GRCGYC 1 cut(s) 191
BssT1I CCWWGG 1 cut(s) 333
Bst4CI ACNGT 2 cut(s) 258, 535
BstACI GRCGYC 1 cut(s) 191
BstBI TTCGAA 1 cut(s) 239
BstC8I GCNNGC 1 cut(s) 124
BstDEI CTNAG 1 cut(s) 527
BstDSI CCRYGG 2 cut(s) 268, 296
BstF5I GGATG 1 cut(s) 704
BstFNI CGCG 5 cut(s) 270, 280, 289, 298, 643
BstHHI GCGC 1 cut(s) 95
BstKTI GATC 3 cut(s) 454, 628, 687
BstMAI GTCTC 2 cut(s) 201, 375
BstMBI GATC 3 cut(s) 451, 625, 684
BstMCI CGRYCG 1 cut(s) 231
BstMWI GCNNNNNNNGC 6 cut(s) 104, 123, 286, 292, 295, 640
BstNSI RCATGY 1 cut(s) 29
BstSLI GKGCMC 1 cut(s) 141
BstUI CGCG 5 cut(s) 270, 280, 289, 298, 643
BstV1I GCAGC 6 cut(s) 50, 82, 414, 436, 493, 515
BstV2I GAAGAC 3 cut(s) 191, 248, 423
BstXI CCANNNNNNTGG 1 cut(s) 154
Bsu15I ATCGAT 1 cut(s) 628
BsuRI GGCC 3 cut(s) 158, 594, 674
BsuTUI ATCGAT 1 cut(s) 628
BtgI CCRYGG 2 cut(s) 268, 296
BtrI CACGTC 2 cut(s) 136, 651
BtsCI GGATG 1 cut(s) 704
BtsIMutI CAGTG 1 cut(s) 694
BveI ACCTGC 1 cut(s) 352
Cac8I GCNNGC 1 cut(s) 124
CaiI CAGNNNCTG 1 cut(s) 427
CfoI GCGC 1 cut(s) 95
Cfr13I GGNCC 2 cut(s) 157, 672
Cfr42I CCGCGG 2 cut(s) 271, 299
ClaI ATCGAT 1 cut(s) 628
CseI GACGC 2 cut(s) 113, 180
Csp6I GTAC 3 cut(s) 88, 397, 531
CviAII CATG 5 cut(s) 26, 313, 323, 596, 622
CviQI GTAC 3 cut(s) 88, 397, 531
DdeI CTNAG 1 cut(s) 527
DpnI GATC 3 cut(s) 453, 627, 686
DpnII GATC 3 cut(s) 451, 625, 684
DrdI GACNNNNNNGTC 1 cut(s) 193
DseDI GACNNNNNNGTC 1 cut(s) 193
EaeI YGGCCR 1 cut(s) 592
EciI GGCGGA 1 cut(s) 133
Ecl136II GAGCTC 1 cut(s) 80
Eco130I CCWWGG 1 cut(s) 333
Eco24I GRGCYC 1 cut(s) 82
Eco53kI GAGCTC 1 cut(s) 80
Eco88I CYCGRG 1 cut(s) 48
EcoICRI GAGCTC 1 cut(s) 80
EcoO109I RGGNCCY 1 cut(s) 672
EcoRI GAATTC 1 cut(s) 4
EcoT14I CCWWGG 1 cut(s) 333
EcoT38I GRGCYC 1 cut(s) 82
ErhI CCWWGG 1 cut(s) 333
Esp3I CGTCTC 2 cut(s) 201, 375
FaeI CATG 5 cut(s) 29, 316, 326, 599, 625
FaiI YATR 8 cut(s) 27, 68, 115, 314, 324, 435, 597, 623
FalI AAGNNNNNCTT 2 cut(s) 510, 542
FatI CATG 5 cut(s) 25, 312, 322, 595, 621
FauI CCCGC 3 cut(s) 263, 291, 296
FriOI GRGCYC 1 cut(s) 82
FspBI CTAG 3 cut(s) 9, 207, 492
GlaI GCGC 1 cut(s) 94
GsaI CCCAGC 1 cut(s) 667
HaeIII GGCC 3 cut(s) 158, 594, 674
HapII CCGG 2 cut(s) 346, 368
HgaI GACGC 2 cut(s) 113, 180
HhaI GCGC 1 cut(s) 95
Hin1I GRCGYC 1 cut(s) 191
Hin1II CATG 5 cut(s) 29, 316, 326, 599, 625
Hin6I GCGC 1 cut(s) 93
HinP1I GCGC 1 cut(s) 93
HinfI GANTC 2 cut(s) 128, 342
HpaII CCGG 2 cut(s) 346, 368
Hpy166II GTNNAC 2 cut(s) 139, 276
Hpy188I TCNGA 4 cut(s) 133, 444, 541, 615
Hpy188III TCNNGA 3 cut(s) 181, 346, 368
Hpy8I GTNNAC 2 cut(s) 139, 276
Hpy99I CGWCG 6 cut(s) 35, 107, 137, 165, 196, 577
HpyAV CCTTC 2 cut(s) 332, 697
HpyCH4III ACNGT 2 cut(s) 258, 535
HpyCH4IV ACGT 2 cut(s) 135, 650
HpyCH4V TGCA 4 cut(s) 66, 139, 474, 503
HpyF10VI GCNNNNNNNGC 6 cut(s) 104, 123, 286, 292, 295, 640
HpyF3I CTNAG 1 cut(s) 527
HpySE526I ACGT 2 cut(s) 135, 650
Hsp92I GRCGYC 1 cut(s) 191
Hsp92II CATG 5 cut(s) 29, 316, 326, 599, 625
HspAI GCGC 1 cut(s) 93
Kpn2I TCCGGA 2 cut(s) 345, 367
KspI CCGCGG 2 cut(s) 271, 299
Kzo9I GATC 3 cut(s) 451, 625, 684
LmnI GCTCC 1 cut(s) 466
LpnPI CCDG 8 cut(s) 136, 347, 359, 365, 381, 434, 468, 649
Lsp1109I GCAGC 6 cut(s) 50, 82, 414, 436, 493, 515
LweI GCATC 1 cut(s) 490
MaeI CTAG 3 cut(s) 9, 207, 492
MaeII ACGT 2 cut(s) 135, 650
MaeIII GTNAC 4 cut(s) 56, 476, 485, 587
MalI GATC 3 cut(s) 453, 627, 686
MboI GATC 3 cut(s) 451, 625, 684
MboII GAAGA 5 cut(s) 196, 253, 416, 428, 509
MfeI CAATTG 1 cut(s) 152
MhlI GDGCHC 2 cut(s) 82, 141
MluCI AATT 4 cut(s) 4, 152, 511, 583
MlyI GAGTC 1 cut(s) 122
MmeI TCCRAC 2 cut(s) 156, 529
MnlI CCTC 4 cut(s) 256, 406, 662, 685
MroI TCCGGA 2 cut(s) 345, 367
MroXI GAANNNNTTC 1 cut(s) 174
MspA1I CMGCKG 6 cut(s) 63, 233, 270, 298, 427, 506
MspI CCGG 2 cut(s) 346, 368
MunI CAATTG 1 cut(s) 152
MvnI CGCG 5 cut(s) 270, 280, 289, 298, 643
MwoI GCNNNNNNNGC 6 cut(s) 104, 123, 286, 292, 295, 640
NdeII GATC 3 cut(s) 451, 625, 684
NlaIII CATG 5 cut(s) 29, 316, 326, 599, 625
NlaIV GGNNCC 6 cut(s) 255, 266, 350, 366, 441, 673
NmeAIII GCCGAG 1 cut(s) 227
NmuCI GTSAC 1 cut(s) 56
NspI RCATGY 1 cut(s) 29
NspV TTCGAA 1 cut(s) 239
PcsI WCGNNNNNNNCGW 3 cut(s) 254, 578, 647
PdmI GAANNNNTTC 1 cut(s) 174
PfeI GAWTC 1 cut(s) 342
Pfl23II CGTACG 1 cut(s) 396
PflFI GACNNNGTC 1 cut(s) 190
PleI GAGTC 1 cut(s) 122
PpsI GAGTC 1 cut(s) 122
Psp124BI GAGCTC 1 cut(s) 82
PspFI CCCAGC 1 cut(s) 663
PspLI CGTACG 1 cut(s) 396
PspN4I GGNNCC 6 cut(s) 255, 266, 350, 366, 441, 673
PspPI GGNCC 2 cut(s) 157, 672
PstNI CAGNNNCTG 1 cut(s) 427
PsyI GACNNNGTC 1 cut(s) 190
PvuII CAGCTG 3 cut(s) 63, 427, 506
RsaI GTAC 3 cut(s) 89, 398, 532
RsaNI GTAC 3 cut(s) 88, 397, 531
SacI GAGCTC 1 cut(s) 82
SacII CCGCGG 2 cut(s) 271, 299
Sau3AI GATC 3 cut(s) 451, 625, 684
Sau96I GGNCC 2 cut(s) 157, 672
ScaI AGTACT 1 cut(s) 532
SchI GAGTC 1 cut(s) 122
SduI GDGCHC 2 cut(s) 82, 141
SfaNI GCATC 1 cut(s) 490
Sfr303I CCGCGG 2 cut(s) 271, 299
SfuI TTCGAA 1 cut(s) 239
SgrBI CCGCGG 2 cut(s) 271, 299
Sse9I AATT 4 cut(s) 4, 152, 511, 583
SspMI CTAG 3 cut(s) 9, 207, 492
SstI GAGCTC 1 cut(s) 82
StyI CCWWGG 1 cut(s) 333
TaaI ACNGT 2 cut(s) 258, 535
TaiI ACGT 2 cut(s) 138, 653
TaqI TCGA 3 cut(s) 239, 450, 628
TasI AATT 4 cut(s) 4, 152, 511, 583
TatI WGTACW 1 cut(s) 530
TauI GCSGC 4 cut(s) 254, 289, 298, 646
TfiI GAWTC 1 cut(s) 342
TscAI CASTG 1 cut(s) 694
TseFI GTSAC 1 cut(s) 56
TseI GCWGC 6 cut(s) 63, 95, 424, 427, 503, 506
Tsp45I GTSAC 1 cut(s) 56
TspDTI ATGAA 2 cut(s) 17, 417
TspGWI ACGGA 1 cut(s) 444
TspRI CASTG 1 cut(s) 694
Tth111I GACNNNGTC 1 cut(s) 190
VneI GTGCAC 1 cut(s) 137
XapI RAATTY 1 cut(s) 4
XceI RCATGY 1 cut(s) 29
XmnI GAANNNNTTC 1 cut(s) 174
XspI CTAG 3 cut(s) 9, 207, 492
ZrmI AGTACT 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.