RchiOBHm_Chr4g0435191

Zinc finger protein CONSTANS-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0012185
Physical Location & Seq
Reverse (-)
58765434 .. 58767212
1779 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40362

Sequence Viewer

Length: 1341 bp
ATGATTTCAAACAAGAAAGCAGCTGCCTCTGCTTCTGCGGCAACAGCGAACGTCGTTGGCGGAAAGACGGCGAGAGCGTGTGACAGCTGTATTAAGAAGAGAGCTAGATGGTACTGTGCTGCTGACGATGCTTTTCTCTGTCAAGCGTGCGACTCCTCCGTCCACTCGGCGAACCAGTTGGCACGCAGACACGAACGTGTCCGGTTGAAAACGGCGTCGTCGATTCTCAAGTCCTCGACCAACTCCGGGAGTTGTGATAAATCAGAAGTGCCATCATGGCACCGAGGGTTCACGAAAAAGGCGCGGACGCCACGGCACGGCAAGTCCGGTACTCATCGGAATCGGTTTCCTCTTGTTCCGGAGGTCGGAGCGGACGACCTTTCGTATGAGGAGAATGAGGAGGAGCAGCTTCTGTATAGGGTTCCCGTATACGATCCCTTTGTTGCGGAGCTCTGCACTTCTCCTAATTCCAACACCGTTTCCAACGAATCCAAAGCGGTTTCGAAGCCGAATTACTCCCGTGTGGGAAGTGGAAATTCATCTTCGTCGCATGGTTTTGATCTTCCCAGTGATATGGATCTTGCGAATTTTGCAGCTGATGTTGATAGCTTATTGGGGAGAGGGCTAGATGACGATAAGTGTTTTGGCATGGAAGGGTTGGGGTTATTGGATTGTCATGAAAAGGAATCCACAACAACGGAGGAGGGTAGTACCGGAAGAGTGAAGCTTGAAGACGAGGAAGATGCTTATATGGAGTGCCAAGCTGAGACGGAGATCGATCTGATGAGGGAGCCGTTTGTGTTGAACTTTGAAGACTACGACGACTCGCCTGCCTCCTGCGCTGAAGATGAGGAGGATGATGACAAATTGGGAGTGACAATGAGTCATGGCGAGATGAAGAGTAGTAGTGAAGAGCATAAAAGTTGCAAGAAGAGGAAGATATTTTTGAGGCTGGACTACGATGCTGTGATTACCAAATGGGATAGCGAAAGTAAAGGATCTCCGTGGACATTCGGAGAAAGGCCCGATTTGGACTCCGATTGCTTGCCTGACTCCATGGGCACGTCAAATGGAGCTGAATTAATTCATTACCCTTATGGAGATTTGAATGGAGTAATTGGATTACATCCGGCAATGACTGACGGAGGGAGGGAGGCGAGGGTATCAAGGTACAGAGAAAAGAGGAGGACGAGGCTGTTCTCGAAGAAAATAAGATACGAAGTTCGGAAGCTGAATGCTGAGAAGAGGCCTAGAATGAAAGGAAGGTTTGTAAAGAGAGCTTCGTTTGCAGCTTCAGGATCTACATTTCCTACGCTTAATAAAGAAGTCATGGACAGCTAA

Protein Analysis

446

Amino Acids

49.45

Weight (kDa)

5.78

Isoelectric Point (pI)

54.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-B_box PF00643 24 - 67 2.5e-07 B-box zinc finger
CCT PF06203 384 - 426 6.1e-17 CCT motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012185)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25440 AT1G68520
fragaria_vesca FvH4_4g27390
malus_domestica MD13G1042200.v1.1 MD16G1043000.v1.1
prunus_persica Prupe.1G310900_v2.0.a1
pyrus_communis pycom13g03650 pycom16g03710
rosa_chinensis RchiOBHm_Chr4g0435191
rosa_laevigata RLG00000006610
rosa_multiflora Rmu_ssc0000339.1_g000030
rosa_roxburghii Rroxscaffold_5G00376410
rosa_rugosa Rorug04G0282900 Rorug04G0283000
rosa_samantha Rh4AG338700 Rh4BG346800 Rh4CG361400 Rh4DG341200
rosa_wichuraiana Rw4G029540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 158
AccB1I GGYRCC 1 cut(s) 279
AccBSI CCGCTC 1 cut(s) 371
AccI GTMKAC 1 cut(s) 429
AccII CGCG 1 cut(s) 304
AccIII TCCGGA 1 cut(s) 358
AciI CCGC 6 cut(s) 38, 60, 304, 371, 446, 497
AclWI GGATC 4 cut(s) 428, 585, 1006, 1306
AcsI RAATTY 2 cut(s) 535, 586
AcuI CTGAAG 2 cut(s) 864, 1278
AcyI GRCGYC 2 cut(s) 215, 308
AfaI GTAC 4 cut(s) 113, 331, 712, 1172
AfiI CCNNNNNNNGG 3 cut(s) 246, 317, 365
AflIII ACRYGT 1 cut(s) 196
AgsI TTSAA 6 cut(s) 9, 208, 731, 805, 812, 1108
AhdI GACNNNNNGTC 1 cut(s) 882
AjiI CACGTC 1 cut(s) 1065
AleI CACNNNNGTG 1 cut(s) 195
Alw21I GWGCWC 1 cut(s) 453
Alw26I GTCTC 1 cut(s) 761
AlwI GGATC 4 cut(s) 428, 585, 1006, 1306
AlwNI CAGNNNCTG 1 cut(s) 412
Aor13HI TCCGGA 1 cut(s) 358
AoxI GGCC 2 cut(s) 1022, 1247
ApeKI GCWGC 6 cut(s) 20, 23, 119, 406, 593, 1289
ApoI RAATTY 2 cut(s) 535, 586
AseI ATTAAT 1 cut(s) 1082
Asp700I GAANNNNTTC 1 cut(s) 1083
AspLEI GCGC 2 cut(s) 304, 842
AspS9I GGNCC 1 cut(s) 1023
AsuC2I CCSGG 1 cut(s) 247
AsuII TTCGAA 1 cut(s) 503
BaeGI GKGCMC 1 cut(s) 1064
BanI GGYRCC 1 cut(s) 279
BanII GRGCYC 1 cut(s) 453
BarI GAAGNNNNNNTAC 2 cut(s) 497, 529
BbsI GAAGAC 2 cut(s) 738, 819
Bbv12I GWGCWC 1 cut(s) 453
BbvI GCAGC 6 cut(s) 10, 32, 106, 418, 605, 1301
BccI CCATC 2 cut(s) 102, 280
BceAI ACGGC 5 cut(s) 84, 228, 329, 334, 778
BcgI CGANNNNNNTGC 4 cut(s) 725, 759, 812, 846
BcnI CCSGG 1 cut(s) 247
BcoDI GTCTC 1 cut(s) 761
BfaI CTAG 3 cut(s) 105, 626, 1251
BglI GCCNNNNNGGC 1 cut(s) 277
BisI GCNGC 7 cut(s) 21, 24, 39, 120, 407, 594, 1290
BlsI GCNGC 7 cut(s) 22, 25, 40, 121, 408, 595, 1291
Bme1390I CCNGG 1 cut(s) 247
BmeRI GACNNNNNGTC 1 cut(s) 882
BmgBI CACGTC 1 cut(s) 1065
BmgT120I GGNCC 1 cut(s) 1023
BmiI GGNNCC 3 cut(s) 281, 423, 792
BmrFI CCNGG 1 cut(s) 247
BmrI ACTGGG 1 cut(s) 561
BmsI GCATC 3 cut(s) 118, 733, 952
BmuI ACTGGG 1 cut(s) 561
BpiI GAAGAC 2 cut(s) 738, 819
Bpu14I TTCGAA 1 cut(s) 503
BpuEI CTTGAG 1 cut(s) 212
BpuMI CCSGG 1 cut(s) 247
Bsa29I ATCGAT 1 cut(s) 777
BsaBI GATNNNNATC 1 cut(s) 576
BsaHI GRCGYC 2 cut(s) 215, 308
BsaJI CCNNGG 4 cut(s) 283, 311, 1004, 1056
BsaWI WCCGGW 4 cut(s) 201, 326, 358, 713
Bsc4I CCNNNNNNNGG 3 cut(s) 246, 317, 365
Bse1I ACTGG 2 cut(s) 175, 567
Bse3DI GCAATG 1 cut(s) 1140
Bse8I GATNNNNATC 1 cut(s) 576
BseAI TCCGGA 1 cut(s) 358
BseCI ATCGAT 1 cut(s) 777
BseDI CCNNGG 4 cut(s) 283, 311, 1004, 1056
BseGI GGATG 2 cut(s) 862, 1126
BseJI GATNNNNATC 1 cut(s) 576
BseLI CCNNNNNNNGG 3 cut(s) 246, 317, 365
BseMI GCAATG 1 cut(s) 1140
BseMII CTCAG 2 cut(s) 756, 1230
BseNI ACTGG 2 cut(s) 175, 567
BseRI GAGGAG 7 cut(s) 145, 404, 413, 416, 716, 866, 1198
BseSI GKGCMC 1 cut(s) 1064
BseXI GCAGC 6 cut(s) 10, 32, 106, 418, 605, 1301
BsgI GTGCAG 1 cut(s) 439
Bsh1236I CGCG 1 cut(s) 304
BshFI GGCC 2 cut(s) 1024, 1249
BshNI GGYRCC 1 cut(s) 279
BshVI ATCGAT 1 cut(s) 777
BsiHKAI GWGCWC 1 cut(s) 453
BsiSI CCGG 6 cut(s) 202, 246, 327, 359, 714, 1130
BslI CCNNNNNNNGG 3 cut(s) 246, 317, 365
BsmAI GTCTC 1 cut(s) 761
BsmBI CGTCTC 1 cut(s) 761
BsmI GAATGC 1 cut(s) 1240
BsnI GGCC 2 cut(s) 1024, 1249
Bsp119I TTCGAA 1 cut(s) 503
Bsp1286I GDGCHC 2 cut(s) 453, 1064
Bsp13I TCCGGA 1 cut(s) 358
Bsp143I GATC 7 cut(s) 433, 559, 577, 774, 778, 998, 1298
Bsp19I CCATGG 1 cut(s) 1056
BspACI CCGC 6 cut(s) 38, 60, 304, 371, 446, 497
BspANI GGCC 2 cut(s) 1024, 1249
BspCNI CTCAG 2 cut(s) 757, 1231
BspDI ATCGAT 1 cut(s) 777
BspEI TCCGGA 1 cut(s) 358
BspFNI CGCG 1 cut(s) 304
BspHI TCATGA 1 cut(s) 676
BspLI GGNNCC 3 cut(s) 281, 423, 792
BspPI GGATC 4 cut(s) 428, 585, 1006, 1306
BspQI GCTCTTC 1 cut(s) 906
BspT104I TTCGAA 1 cut(s) 503
BspT107I GGYRCC 1 cut(s) 279
BsrBI CCGCTC 1 cut(s) 371
BsrDI GCAATG 1 cut(s) 1140
BsrI ACTGG 2 cut(s) 175, 567
BssECI CCNNGG 4 cut(s) 283, 311, 1004, 1056
BssMI GATC 7 cut(s) 433, 559, 577, 774, 778, 998, 1298
BssNAI GTATAC 1 cut(s) 430
BssNI GRCGYC 2 cut(s) 215, 308
BssT1I CCWWGG 1 cut(s) 1056
Bst1107I GTATAC 1 cut(s) 430
Bst4CI ACNGT 2 cut(s) 116, 478
Bst6I CTCTTC 6 cut(s) 92, 712, 893, 906, 926, 1238
BstACI GRCGYC 2 cut(s) 215, 308
BstBI TTCGAA 1 cut(s) 503
BstC8I GCNNGC 4 cut(s) 148, 184, 831, 1046
BstDEI CTNAG 2 cut(s) 765, 1239
BstDSI CCRYGG 3 cut(s) 311, 1004, 1056
BstF5I GGATG 2 cut(s) 862, 1126
BstFNI CGCG 1 cut(s) 304
BstHHI GCGC 2 cut(s) 304, 842
BstKTI GATC 7 cut(s) 436, 562, 580, 777, 781, 1001, 1301
BstMAI GTCTC 1 cut(s) 761
BstMBI GATC 7 cut(s) 433, 559, 577, 774, 778, 998, 1298
BstMWI GCNNNNNNNGC 8 cut(s) 29, 38, 44, 128, 277, 590, 839, 1286
BstSCI CCNGG 1 cut(s) 245
BstSLI GKGCMC 1 cut(s) 1064
BstUI CGCG 1 cut(s) 304
BstV1I GCAGC 6 cut(s) 10, 32, 106, 418, 605, 1301
BstV2I GAAGAC 2 cut(s) 738, 819
BstX2I RGATCY 3 cut(s) 577, 998, 1298
BstXI CCANNNNNNTGG 1 cut(s) 574
BstYI RGATCY 3 cut(s) 577, 998, 1298
BstZ17I GTATAC 1 cut(s) 430
Bsu15I ATCGAT 1 cut(s) 777
BsuRI GGCC 2 cut(s) 1024, 1249
BsuTUI ATCGAT 1 cut(s) 777
BtgI CCRYGG 3 cut(s) 311, 1004, 1056
BtrI CACGTC 1 cut(s) 1065
BtsCI GGATG 2 cut(s) 862, 1126
BtsIMutI CAGTG 1 cut(s) 574
Cac8I GCNNGC 4 cut(s) 148, 184, 831, 1046
CaiI CAGNNNCTG 1 cut(s) 412
CciI TCATGA 1 cut(s) 676
CfoI GCGC 2 cut(s) 304, 842
Cfr13I GGNCC 1 cut(s) 1023
ClaI ATCGAT 1 cut(s) 777
CseI GACGC 2 cut(s) 204, 316
Csp6I GTAC 4 cut(s) 112, 330, 711, 1171
CviAII CATG 7 cut(s) 276, 551, 649, 677, 887, 1057, 1330
CviQI GTAC 4 cut(s) 112, 330, 711, 1171
DdeI CTNAG 2 cut(s) 765, 1239
DpnI GATC 7 cut(s) 435, 561, 579, 776, 780, 1000, 1300
DpnII GATC 7 cut(s) 433, 559, 577, 774, 778, 998, 1298
DrdI GACNNNNNNGTC 1 cut(s) 158
DriI GACNNNNNGTC 1 cut(s) 882
DseDI GACNNNNNNGTC 1 cut(s) 158
Eam1104I CTCTTC 6 cut(s) 92, 712, 893, 906, 926, 1238
Eam1105I GACNNNNNGTC 1 cut(s) 882
EarI CTCTTC 6 cut(s) 92, 712, 893, 906, 926, 1238
EciI GGCGGA 1 cut(s) 75
Ecl136II GAGCTC 1 cut(s) 451
Eco130I CCWWGG 1 cut(s) 1056
Eco147I AGGCCT 1 cut(s) 1249
Eco24I GRGCYC 1 cut(s) 453
Eco53kI GAGCTC 1 cut(s) 451
Eco57I CTGAAG 2 cut(s) 864, 1278
EcoICRI GAGCTC 1 cut(s) 451
EcoT14I CCWWGG 1 cut(s) 1056
EcoT38I GRGCYC 1 cut(s) 453
ErhI CCWWGG 1 cut(s) 1056
Esp3I CGTCTC 1 cut(s) 761
FaeI CATG 7 cut(s) 279, 554, 652, 680, 890, 1060, 1333
FatI CATG 7 cut(s) 275, 550, 648, 676, 886, 1056, 1329
FblI GTMKAC 1 cut(s) 429
Fnu4HI GCNGC 7 cut(s) 21, 24, 39, 120, 407, 594, 1290
FokI GGATG 2 cut(s) 869, 1113
FriOI GRGCYC 1 cut(s) 453
Fsp4HI GCNGC 7 cut(s) 21, 24, 39, 120, 407, 594, 1290
FspBI CTAG 3 cut(s) 105, 626, 1251
GlaI GCGC 2 cut(s) 303, 841
GluI GCNGC 7 cut(s) 21, 24, 39, 120, 407, 594, 1290
HaeIII GGCC 2 cut(s) 1024, 1249
HapII CCGG 6 cut(s) 202, 246, 327, 359, 714, 1130
HgaI GACGC 2 cut(s) 204, 316
HhaI GCGC 2 cut(s) 304, 842
Hin1I GRCGYC 2 cut(s) 215, 308
Hin1II CATG 7 cut(s) 279, 554, 652, 680, 890, 1060, 1333
Hin6I GCGC 2 cut(s) 302, 840
HinP1I GCGC 2 cut(s) 302, 840
HindIII AAGCTT 1 cut(s) 725
HinfI GANTC 9 cut(s) 152, 223, 340, 488, 686, 824, 883, 1034, 1052
HpaII CCGG 6 cut(s) 202, 246, 327, 359, 714, 1130
Hpy166II GTNNAC 4 cut(s) 163, 291, 430, 1008
Hpy188I TCNGA 7 cut(s) 265, 339, 368, 783, 1016, 1039, 1227
Hpy188III TCNNGA 5 cut(s) 292, 359, 677, 1201, 1296
Hpy8I GTNNAC 4 cut(s) 163, 291, 430, 1008
Hpy99I CGWCG 5 cut(s) 56, 220, 223, 550, 824
HpyAV CCTTC 2 cut(s) 647, 1257
HpyCH4III ACNGT 2 cut(s) 116, 478
HpyCH4IV ACGT 3 cut(s) 51, 196, 1064
HpyCH4V TGCA 4 cut(s) 456, 593, 927, 1289
HpyF10VI GCNNNNNNNGC 8 cut(s) 29, 38, 44, 128, 277, 590, 839, 1286
HpyF3I CTNAG 2 cut(s) 765, 1239
HpySE526I ACGT 3 cut(s) 51, 196, 1064
Hsp92I GRCGYC 2 cut(s) 215, 308
Hsp92II CATG 7 cut(s) 279, 554, 652, 680, 890, 1060, 1333
HspAI GCGC 2 cut(s) 302, 840
Kpn2I TCCGGA 1 cut(s) 358
Kzo9I GATC 7 cut(s) 433, 559, 577, 774, 778, 998, 1298
LguI GCTCTTC 1 cut(s) 906
LmnI GCTCC 5 cut(s) 368, 403, 448, 790, 1073
Lsp1109I GCAGC 6 cut(s) 10, 32, 106, 418, 605, 1301
LweI GCATC 3 cut(s) 118, 733, 952
MaeI CTAG 3 cut(s) 105, 626, 1251
MaeII ACGT 3 cut(s) 51, 196, 1064
MaeIII GTNAC 2 cut(s) 80, 874
MalI GATC 7 cut(s) 435, 561, 579, 776, 780, 1000, 1300
MbiI CCGCTC 1 cut(s) 371
MboI GATC 7 cut(s) 433, 559, 577, 774, 778, 998, 1298
MflI RGATCY 3 cut(s) 577, 998, 1298
MhlI GDGCHC 2 cut(s) 453, 1064
MluCI AATT 8 cut(s) 466, 511, 535, 586, 866, 1079, 1083, 1116
MlyI GAGTC 5 cut(s) 146, 818, 892, 1028, 1046
MmeI TCCRAC 3 cut(s) 346, 495, 507
MroI TCCGGA 1 cut(s) 358
MroXI GAANNNNTTC 1 cut(s) 1083
MseI TTAA 3 cut(s) 93, 1082, 1317
MslI CAYNNNNRTG 1 cut(s) 195
MspA1I CMGCKG 3 cut(s) 23, 87, 596
MspI CCGG 6 cut(s) 202, 246, 327, 359, 714, 1130
MspR9I CCNGG 1 cut(s) 247
Mva1269I GAATGC 1 cut(s) 1240
MvnI CGCG 1 cut(s) 304
MwoI GCNNNNNNNGC 8 cut(s) 29, 38, 44, 128, 277, 590, 839, 1286
NciI CCSGG 1 cut(s) 247
NcoI CCATGG 1 cut(s) 1056
NdeII GATC 7 cut(s) 433, 559, 577, 774, 778, 998, 1298
NlaIII CATG 7 cut(s) 279, 554, 652, 680, 890, 1060, 1333
NlaIV GGNNCC 3 cut(s) 281, 423, 792
NmeAIII GCCGAG 1 cut(s) 146
NmuCI GTSAC 2 cut(s) 80, 874
NspV TTCGAA 1 cut(s) 503
OliI CACNNNNGTG 1 cut(s) 195
PagI TCATGA 1 cut(s) 676
PceI AGGCCT 1 cut(s) 1249
PciSI GCTCTTC 1 cut(s) 906
PcsI WCGNNNNNNNCGW 3 cut(s) 74, 218, 372
PctI GAATGC 1 cut(s) 1240
PdmI GAANNNNTTC 1 cut(s) 1083
PfeI GAWTC 4 cut(s) 223, 340, 488, 686
PfoI TCCNGGA 1 cut(s) 245
PkrI GCNGC 7 cut(s) 22, 25, 40, 121, 408, 595, 1291
PleI GAGTC 5 cut(s) 146, 818, 891, 1028, 1046
PpsI GAGTC 5 cut(s) 146, 818, 891, 1028, 1046
PshBI ATTAAT 1 cut(s) 1082
Psp124BI GAGCTC 1 cut(s) 453
PspN4I GGNNCC 3 cut(s) 281, 423, 792
PspPI GGNCC 1 cut(s) 1023
PstNI CAGNNNCTG 1 cut(s) 412
PsuI RGATCY 3 cut(s) 577, 998, 1298
PvuII CAGCTG 3 cut(s) 23, 87, 596
RsaI GTAC 4 cut(s) 113, 331, 712, 1172
RsaNI GTAC 4 cut(s) 112, 330, 711, 1171
RseI CAYNNNNRTG 1 cut(s) 195
SacI GAGCTC 1 cut(s) 453
SapI GCTCTTC 1 cut(s) 906
SaqAI TTAA 3 cut(s) 93, 1082, 1317
SatI GCNGC 7 cut(s) 21, 24, 39, 120, 407, 594, 1290
Sau3AI GATC 7 cut(s) 433, 559, 577, 774, 778, 998, 1298
Sau96I GGNCC 1 cut(s) 1023
SchI GAGTC 5 cut(s) 146, 818, 892, 1028, 1046
ScrFI CCNGG 1 cut(s) 247
SduI GDGCHC 2 cut(s) 453, 1064
SfaNI GCATC 3 cut(s) 118, 733, 952
SfuI TTCGAA 1 cut(s) 503
SmiMI CAYNNNNRTG 1 cut(s) 195
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
Sse9I AATT 8 cut(s) 466, 511, 535, 586, 866, 1079, 1083, 1116
SseBI AGGCCT 1 cut(s) 1249
SsiI CCGC 6 cut(s) 38, 60, 304, 371, 446, 497
SspMI CTAG 3 cut(s) 105, 626, 1251
SstI GAGCTC 1 cut(s) 453
StuI AGGCCT 1 cut(s) 1249
StyD4I CCNGG 1 cut(s) 245
StyI CCWWGG 1 cut(s) 1056
TaaI ACNGT 2 cut(s) 116, 478
TaiI ACGT 3 cut(s) 54, 199, 1067
TaqI TCGA 5 cut(s) 221, 236, 503, 777, 1202
TasI AATT 8 cut(s) 466, 511, 535, 586, 866, 1079, 1083, 1116
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 4 cut(s) 223, 340, 488, 686
Tru1I TTAA 3 cut(s) 93, 1082, 1317
Tru9I TTAA 3 cut(s) 93, 1082, 1317
TscAI CASTG 1 cut(s) 574
TseFI GTSAC 2 cut(s) 80, 874
TseI GCWGC 6 cut(s) 20, 23, 119, 406, 593, 1289
Tsp45I GTSAC 2 cut(s) 80, 874
TspDTI ATGAA 5 cut(s) 528, 693, 911, 1076, 1271
TspGWI ACGGA 5 cut(s) 148, 713, 785, 993, 1158
TspRI CASTG 1 cut(s) 574
VspI ATTAAT 1 cut(s) 1082
XapI RAATTY 2 cut(s) 535, 586
XmiI GTMKAC 1 cut(s) 429
XmnI GAANNNNTTC 1 cut(s) 1083
XspI CTAG 3 cut(s) 105, 626, 1251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.