FvH4_4g27661

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016110
Physical Location & Seq
Forward (+)
28556302 .. 28558011
1710 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27661.t1

Sequence Viewer

Length: 984 bp
ATGGCCACCGTTACTACGACATCTCAGCTTCTTCTCTGCACCAACCGCGCCATTTTCGCGACCCGGGCCCGATTCCGAAAACCCGACCCGGTTGCATTGGAAGCACATTTTTCGGCACCACAGTCGCTCAGGGTCTCAAAGCTGAAGCCACTAGTTGTGTGCCACGCGTCACGTCGGAAACCCACCACAGCTGTCCAACCAGAGACTGGAAATGAAGATTCCCGGCGACTGTTTCAGACTGGTCTCTGGGTTGCTGAGGCAGTCTACATTTTATGGCTGTTTCTTCTTCCTTATGCTCCTGGAGACCCGGTGTGGGCTATAAGTTCAGAAACAGTGAATTCTCTTCTGGGCCTCTCTTTGAATTTCTTCTTTGTGTTGCCTCTTTTCAACTCTGTTGGTATTAATTTTATTGATGCCCCAGTTCTTCACCCGATGTCTGAAGGGCTATTCAACTTTGTGATTGGGTGGACGTTCATGTTTGCTCCTTTGCTTTTCACTGATGGGAAGAGGGACAGATACAAGGGGTCTCTTGATGTCTTGTGGGGATTTCAGATGTTCCTTACAAACACATTTTTGATACCTTACATGGCTATTCGGCTGAATGCACCTGAATCAGACTACACTCCCAGCAAGCGCTCACAGCTTGGAGATGTGATGACCAAAGGTGCTAGCATCGTCGGACTTGTTGGTGGAGGGATATGTCTGATATCTGCATTGTGGGCACTTTATGGACGGATGGACGGCAACTTTGGCGGCATTTCTGAGCGTTGGGAGTTTTTGATCAGCTATCTAGGATCCGAGAGGCTGGCTTATGCATTCATTTGGGATATAGTTCTTTATAGTGTCTTCCAGCCTTGGTTGATAGGTGACAATCTGCAGAATGTTGAGAGAAGCAAGATTGGTATCGTAAATTACCTTAGATTTGTGCCCGTGATTGGCTTAGTTGCTTACCTTCTTTGTTTGAACCTTGATGAGGAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

328

Amino Acids

36.7

Weight (kDa)

7.65

Isoelectric Point (pI)

43.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25805
fragaria_vesca FvH4_4g27661 FvH4_4g27661
malus_domestica MD13G1044100.v1.1
prunus_persica Prupe.1G308400_v2.0.a1
pyrus_communis pycom13g03870
rosa_chinensis RchiOBHm_Chr4g0435511
rosa_laevigata RLG00000006586
rosa_multiflora Rmu_sc0001940.1_g000030
rosa_roxburghii Rroxscaffold_5G00376740
rosa_rugosa Rorug04G0285800
rosa_samantha Rh4BG349700 Rh4CG364400 Rh4DG344000
rosa_wichuraiana Rw4G029790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 115
AccB7I CCANNNNNTGG 1 cut(s) 206
AccI GTMKAC 1 cut(s) 264
AccII CGCG 3 cut(s) 48, 59, 167
AciI CCGC 2 cut(s) 46, 753
AclWI GGATC 2 cut(s) 789, 802
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 337, 361
AcuI CTGAAG 2 cut(s) 164, 459
AfeI AGCGCT 1 cut(s) 635
AfiI CCNNNNNNNGG 4 cut(s) 206, 313, 935, 973
AflIII ACRYGT 1 cut(s) 165
AgsI TTSAA 4 cut(s) 361, 388, 451, 964
AhlI ACTAGT 1 cut(s) 151
AjiI CACGTC 1 cut(s) 173
AjnI CCWGG 1 cut(s) 298
AluBI AGCT 5 cut(s) 28, 142, 191, 643, 786
AluI AGCT 5 cut(s) 28, 142, 191, 643, 786
Alw26I GTCTC 5 cut(s) 139, 197, 248, 297, 531
AlwI GGATC 2 cut(s) 789, 802
AlwNI CAGNNNCTG 1 cut(s) 206
Ama87I CYCGRG 1 cut(s) 63
Aor51HI AGCGCT 1 cut(s) 635
AoxI GGCC 3 cut(s) 3, 66, 349
ApaI GGGCCC 1 cut(s) 70
ApoI RAATTY 2 cut(s) 337, 361
ArsI GACNNNNNNTTYG 2 cut(s) 731, 763
AseI ATTAAT 1 cut(s) 402
Asp700I GAANNNNTTC 1 cut(s) 365
AspLEI GCGC 2 cut(s) 50, 636
AspS9I GGNCC 3 cut(s) 66, 67, 349
AsuC2I CCSGG 5 cut(s) 64, 65, 89, 223, 308
AsuHPI GGTGA 2 cut(s) 419, 878
AsuNHI GCTAGC 1 cut(s) 668
AvaI CYCGRG 1 cut(s) 63
BaeGI GKGCMC 3 cut(s) 70, 724, 930
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 794
BanI GGYRCC 1 cut(s) 115
BanII GRGCYC 1 cut(s) 70
BbsI GAAGAC 1 cut(s) 838
BbvCI CCTCAGC 1 cut(s) 255
BccI CCATC 2 cut(s) 494, 730
BceAI ACGGC 1 cut(s) 757
BcgI CGANNNNNNTGC 6 cut(s) 74, 93, 105, 108, 127, 139
BciT130I CCWGG 1 cut(s) 300
BclI TGATCA 1 cut(s) 780
BcnI CCSGG 5 cut(s) 64, 65, 89, 223, 308
BcoDI GTCTC 5 cut(s) 139, 197, 248, 297, 531
BcuI ACTAGT 1 cut(s) 151
BfaI CTAG 3 cut(s) 152, 669, 791
BfmI CTRYAG 1 cut(s) 875
BfoI RGCGCY 1 cut(s) 637
BisI GCNGC 1 cut(s) 754
BlsI GCNGC 1 cut(s) 755
Bme1390I CCNGG 6 cut(s) 64, 65, 89, 223, 300, 308
BmeT110I CYCGRG 1 cut(s) 63
BmgBI CACGTC 1 cut(s) 173
BmgT120I GGNCC 3 cut(s) 66, 67, 349
BmiI GGNNCC 3 cut(s) 68, 117, 796
BmrFI CCNGG 6 cut(s) 64, 65, 89, 223, 300, 308
BmrI ACTGGG 1 cut(s) 413
BmsI GCATC 2 cut(s) 403, 681
BmtI GCTAGC 1 cut(s) 672
BmuI ACTGGG 1 cut(s) 413
BpiI GAAGAC 1 cut(s) 838
BpmI CTGGAG 1 cut(s) 321
Bpu10I CCTNAGC 2 cut(s) 128, 255
BpuMI CCSGG 5 cut(s) 64, 65, 89, 223, 308
BsaBI GATNNNNATC 1 cut(s) 902
BsaI GGTCTC 4 cut(s) 139, 248, 297, 531
BsaJI CCNNGG 2 cut(s) 63, 854
BsaXI ACNNNNNCTCC 2 cut(s) 294, 324
Bsc4I CCNNNNNNNGG 4 cut(s) 206, 313, 935, 973
Bse1I ACTGG 3 cut(s) 211, 244, 419
Bse8I GATNNNNATC 1 cut(s) 902
BseBI CCWGG 1 cut(s) 300
BseDI CCNNGG 2 cut(s) 63, 854
BseGI GGATG 1 cut(s) 741
BseJI GATNNNNATC 1 cut(s) 902
BseLI CCNNNNNNNGG 4 cut(s) 206, 313, 935, 973
BseMII CTCAG 4 cut(s) 38, 142, 246, 753
BseNI ACTGG 3 cut(s) 211, 244, 419
BseSI GKGCMC 3 cut(s) 70, 724, 930
BseYI CCCAGC 1 cut(s) 626
BsgI GTGCAG 1 cut(s) 22
Bsh1236I CGCG 3 cut(s) 48, 59, 167
BshFI GGCC 3 cut(s) 5, 68, 351
BshNI GGYRCC 1 cut(s) 115
BsiHKCI CYCGRG 1 cut(s) 63
BsiSI CCGG 4 cut(s) 64, 89, 223, 308
BslFI GGGAC 1 cut(s) 524
BslI CCNNNNNNNGG 4 cut(s) 206, 313, 935, 973
BsmAI GTCTC 5 cut(s) 139, 197, 248, 297, 531
BsmFI GGGAC 1 cut(s) 524
BsmI GAATGC 2 cut(s) 607, 815
BsnI GGCC 3 cut(s) 5, 68, 351
Bso31I GGTCTC 4 cut(s) 139, 248, 297, 531
BsoBI CYCGRG 1 cut(s) 63
Bsp120I GGGCCC 1 cut(s) 66
Bsp1286I GDGCHC 3 cut(s) 70, 724, 930
Bsp143I GATC 2 cut(s) 780, 794
Bsp68I TCGCGA 1 cut(s) 59
BspACI CCGC 2 cut(s) 46, 753
BspANI GGCC 3 cut(s) 5, 68, 351
BspCNI CTCAG 4 cut(s) 37, 141, 247, 754
BspFNI CGCG 3 cut(s) 48, 59, 167
BspLI GGNNCC 3 cut(s) 68, 117, 796
BspMAI CTGCAG 1 cut(s) 879
BspOI GCTAGC 1 cut(s) 672
BspPI GGATC 2 cut(s) 789, 802
BspT107I GGYRCC 1 cut(s) 115
BspTNI GGTCTC 4 cut(s) 139, 248, 297, 531
BsrI ACTGG 3 cut(s) 211, 244, 419
BssECI CCNNGG 2 cut(s) 63, 854
BssMI GATC 2 cut(s) 780, 794
BssT1I CCWWGG 1 cut(s) 854
Bst2UI CCWGG 1 cut(s) 300
Bst4CI ACNGT 5 cut(s) 10, 123, 231, 334, 981
Bst6I CTCTTC 2 cut(s) 348, 500
BstC8I GCNNGC 3 cut(s) 632, 670, 807
BstDEI CTNAG 6 cut(s) 24, 128, 255, 762, 917, 940
BstENI CCTNNNNNAGG 1 cut(s) 971
BstF5I GGATG 1 cut(s) 741
BstFNI CGCG 3 cut(s) 48, 59, 167
BstH2I RGCGCY 1 cut(s) 637
BstHHI GCGC 2 cut(s) 50, 636
BstKTI GATC 2 cut(s) 783, 797
BstMAI GTCTC 5 cut(s) 139, 197, 248, 297, 531
BstMBI GATC 2 cut(s) 780, 794
BstMWI GCNNNNNNNGC 7 cut(s) 45, 56, 65, 101, 640, 719, 750
BstNI CCWGG 1 cut(s) 300
BstSCI CCNGG 6 cut(s) 62, 63, 87, 221, 298, 306
BstSFI CTRYAG 1 cut(s) 875
BstSLI GKGCMC 3 cut(s) 70, 724, 930
BstUI CGCG 3 cut(s) 48, 59, 167
BstV2I GAAGAC 1 cut(s) 838
BstX2I RGATCY 1 cut(s) 794
BstYI RGATCY 1 cut(s) 794
BsuRI GGCC 3 cut(s) 5, 68, 351
BtrI CACGTC 1 cut(s) 173
BtsCI GGATG 1 cut(s) 741
BtsIMutI CAGTG 2 cut(s) 339, 495
BtuMI TCGCGA 1 cut(s) 59
Cac8I GCNNGC 3 cut(s) 632, 670, 807
CaiI CAGNNNCTG 1 cut(s) 206
CfoI GCGC 2 cut(s) 50, 636
Cfr13I GGNCC 3 cut(s) 66, 67, 349
Cfr9I CCCGGG 1 cut(s) 63
CseI GACGC 1 cut(s) 156
CviAII CATG 2 cut(s) 475, 586
DdeI CTNAG 6 cut(s) 24, 128, 255, 762, 917, 940
DpnI GATC 2 cut(s) 782, 796
DpnII GATC 2 cut(s) 780, 794
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 348, 500
EarI CTCTTC 2 cut(s) 348, 500
Eco130I CCWWGG 1 cut(s) 854
Eco24I GRGCYC 1 cut(s) 70
Eco31I GGTCTC 4 cut(s) 139, 248, 297, 531
Eco32I GATATC 1 cut(s) 708
Eco47III AGCGCT 1 cut(s) 635
Eco57I CTGAAG 2 cut(s) 164, 459
Eco88I CYCGRG 1 cut(s) 63
EcoNI CCTNNNNNAGG 1 cut(s) 971
EcoRI GAATTC 1 cut(s) 337
EcoRII CCWGG 1 cut(s) 298
EcoRV GATATC 1 cut(s) 708
EcoT14I CCWWGG 1 cut(s) 854
EcoT22I ATGCAT 1 cut(s) 817
EcoT38I GRGCYC 1 cut(s) 70
ErhI CCWWGG 1 cut(s) 854
FaeI CATG 2 cut(s) 478, 589
FaqI GGGAC 1 cut(s) 524
FatI CATG 2 cut(s) 474, 585
FbaI TGATCA 1 cut(s) 780
FblI GTMKAC 1 cut(s) 264
Fnu4HI GCNGC 1 cut(s) 754
FokI GGATG 1 cut(s) 748
FriOI GRGCYC 1 cut(s) 70
Fsp4HI GCNGC 1 cut(s) 754
FspBI CTAG 3 cut(s) 152, 669, 791
GlaI GCGC 2 cut(s) 49, 635
GluI GCNGC 1 cut(s) 754
GsaI CCCAGC 1 cut(s) 630
GsuI CTGGAG 1 cut(s) 321
HaeII RGCGCY 1 cut(s) 637
HaeIII GGCC 3 cut(s) 5, 68, 351
HapII CCGG 4 cut(s) 64, 89, 223, 308
HgaI GACGC 1 cut(s) 156
HhaI GCGC 2 cut(s) 50, 636
Hin1II CATG 2 cut(s) 478, 589
Hin6I GCGC 2 cut(s) 48, 634
HinP1I GCGC 2 cut(s) 48, 634
HinfI GANTC 3 cut(s) 72, 218, 611
HpaII CCGG 4 cut(s) 64, 89, 223, 308
HphI GGTGA 2 cut(s) 419, 878
Hpy166II GTNNAC 2 cut(s) 265, 468
Hpy188III TCNNGA 2 cut(s) 58, 530
Hpy8I GTNNAC 2 cut(s) 265, 468
Hpy99I CGWCG 2 cut(s) 177, 680
HpyAV CCTTC 2 cut(s) 434, 962
HpyCH4III ACNGT 5 cut(s) 10, 123, 231, 334, 981
HpyCH4IV ACGT 2 cut(s) 172, 470
HpyCH4V TGCA 6 cut(s) 39, 95, 605, 713, 815, 877
HpyF10VI GCNNNNNNNGC 7 cut(s) 45, 56, 65, 101, 640, 719, 750
HpyF3I CTNAG 6 cut(s) 24, 128, 255, 762, 917, 940
HpySE526I ACGT 2 cut(s) 172, 470
Hsp92II CATG 2 cut(s) 478, 589
HspAI GCGC 2 cut(s) 48, 634
Ksp22I TGATCA 1 cut(s) 780
Kzo9I GATC 2 cut(s) 780, 794
LmnI GCTCC 2 cut(s) 301, 487
LweI GCATC 2 cut(s) 403, 681
MaeI CTAG 3 cut(s) 152, 669, 791
MaeII ACGT 2 cut(s) 172, 470
MaeIII GTNAC 3 cut(s) 10, 168, 866
MalI GATC 2 cut(s) 782, 796
MboI GATC 2 cut(s) 780, 794
MboII GAAGA 9 cut(s) 23, 227, 275, 278, 335, 358, 416, 517, 838
MflI RGATCY 1 cut(s) 794
MhlI GDGCHC 3 cut(s) 70, 724, 930
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 337, 361, 403, 910
MluI ACGCGT 1 cut(s) 165
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 3 cut(s) 155, 220, 658
MnlI CCTC 7 cut(s) 250, 362, 390, 501, 686, 795, 967
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 817
MroXI GAANNNNTTC 1 cut(s) 365
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 402
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 191
MspI CCGG 4 cut(s) 64, 89, 223, 308
MspR9I CCNGG 6 cut(s) 64, 65, 89, 223, 300, 308
Mva1269I GAATGC 2 cut(s) 607, 815
MvaI CCWGG 1 cut(s) 300
MvnI CGCG 3 cut(s) 48, 59, 167
MwoI GCNNNNNNNGC 7 cut(s) 45, 56, 65, 101, 640, 719, 750
NciI CCSGG 5 cut(s) 64, 65, 89, 223, 308
NdeII GATC 2 cut(s) 780, 794
NheI GCTAGC 1 cut(s) 668
NlaIII CATG 2 cut(s) 478, 589
NlaIV GGNNCC 3 cut(s) 68, 117, 796
NmuCI GTSAC 2 cut(s) 168, 866
NruI TCGCGA 1 cut(s) 59
NsiI ATGCAT 1 cut(s) 817
PctI GAATGC 2 cut(s) 607, 815
PdmI GAANNNNTTC 1 cut(s) 365
PfeI GAWTC 3 cut(s) 72, 218, 611
PflMI CCANNNNNTGG 1 cut(s) 206
PfoI TCCNGGA 1 cut(s) 298
PkrI GCNGC 1 cut(s) 755
PshBI ATTAAT 1 cut(s) 402
Psp6I CCWGG 1 cut(s) 298
PspFI CCCAGC 1 cut(s) 626
PspGI CCWGG 1 cut(s) 298
PspN4I GGNNCC 3 cut(s) 68, 117, 796
PspOMI GGGCCC 1 cut(s) 66
PspPI GGNCC 3 cut(s) 66, 67, 349
PstI CTGCAG 1 cut(s) 879
PstNI CAGNNNCTG 1 cut(s) 206
PsuI RGATCY 1 cut(s) 794
PvuII CAGCTG 1 cut(s) 191
RruI TCGCGA 1 cut(s) 59
SaqAI TTAA 1 cut(s) 402
SatI GCNGC 1 cut(s) 754
Sau3AI GATC 2 cut(s) 780, 794
Sau96I GGNCC 3 cut(s) 66, 67, 349
ScrFI CCNGG 6 cut(s) 64, 65, 89, 223, 300, 308
SduI GDGCHC 3 cut(s) 70, 724, 930
SfaNI GCATC 2 cut(s) 403, 681
SfcI CTRYAG 1 cut(s) 875
SmaI CCCGGG 1 cut(s) 65
SpeI ACTAGT 1 cut(s) 151
Sse9I AATT 4 cut(s) 337, 361, 403, 910
SsiI CCGC 2 cut(s) 46, 753
SspMI CTAG 3 cut(s) 152, 669, 791
StyD4I CCNGG 6 cut(s) 62, 63, 87, 221, 298, 306
StyI CCWWGG 1 cut(s) 854
TaaI ACNGT 5 cut(s) 10, 123, 231, 334, 981
TaiI ACGT 2 cut(s) 175, 473
TasI AATT 4 cut(s) 337, 361, 403, 910
TauI GCSGC 1 cut(s) 756
TfiI GAWTC 3 cut(s) 72, 218, 611
Tru1I TTAA 1 cut(s) 402
Tru9I TTAA 1 cut(s) 402
TscAI CASTG 2 cut(s) 339, 502
TseFI GTSAC 2 cut(s) 168, 866
Tsp45I GTSAC 2 cut(s) 168, 866
TspDTI ATGAA 3 cut(s) 228, 463, 808
TspGWI ACGGA 1 cut(s) 748
TspMI CCCGGG 1 cut(s) 63
TspRI CASTG 2 cut(s) 339, 502
Van91I CCANNNNNTGG 1 cut(s) 206
VspI ATTAAT 1 cut(s) 402
XagI CCTNNNNNAGG 1 cut(s) 971
XapI RAATTY 2 cut(s) 337, 361
XcmI CCANNNNNNNNNTGG 1 cut(s) 203
XmaI CCCGGG 1 cut(s) 63
XmiI GTMKAC 1 cut(s) 264
XmnI GAANNNNTTC 1 cut(s) 365
XspI CTAG 3 cut(s) 152, 669, 791
Zsp2I ATGCAT 1 cut(s) 817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.