MD13G1044100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0016110
Physical Location & Seq
Forward (+)
3069313 .. 3071330
2018 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1044100.v1.1.491

Sequence Viewer

Length: 1026 bp
ATGGCCGTCGTCAGCGCCACGTCTCCGCCTCTGCTCTGCTGCGCCGACCGCCGCGCCTTTTTTTCAACTAGGCCCTCCCTAGGCTCTCGGACCAAGACCCAATTGTGCACACAGAATCCATTTTTGGCGGCACTGGACTCCCAAACGTGGCCTTCACAACCGCCCGCAACCCTCAAAAGGGACTCCAAATTGAAGCCGCTCGTGTGCCGCGCGGATCGCAGGAAACCCACCACCTCCGTCCAGCAGGAGAACGACGGCTCAGAACGGCTGCTTCAGATTGCTCTGTGGCTTGCTGAGGGAGTCTACATTTTGTGGCTTTTTCTTCTTCCCTATGCTCCTGGAGACCCAGTGTGGGCTATTAGTTCGGATACAGTGAACTCTCTTGTGGGGCTCTCTCTTAATTTCTTCTTCATCTTGCCTTTTCTCAACTCCGTAGTTGGTGTTGGTTTGATTGATGCCCCAGTTCTTCACCCGATGTCTGAGGGATTATTCAACTTTGTGATTGGGTGGACATTCATGTTTGCTCCTCTGCTGTTCACGGACCGAAGGAGGGACAGATACAAGTGGTCTCTCGACGCCTTGTGGGGTTTTCAGATGTTTCTCACAAACACTTTTCTAATACCTTACATGGCTATTCGGCTGAATGAGGCTGAGTCTGACTACACTCCGAGCAAGCGCTCACAGCTTGCATCAGTGATGACTAGCGGAGCTCCCATCGTTGGACTTGCTGGTGGAGCAATATGTCTAATATCTGCATTATGGGCTCTTTTTGGCCGGATGGATGGCGACTTTGGAAGCATTTCAGACCGATGGGAGTTCTTGATCGGTTACCTAGGATCGGAGAGGCTGGCTTATGCATTCATTTGGGACATCTGTCTTTACGCCGTCTTCCAGCCCTGGTTGATAGGTGACAATCTGCAAAATGTTGAGAGCAGCAAGATTGGTATTGTAAATTATCTTAGATTTGTACCAGTTGTTGGCTTAGTTGCTTACCTCGTTTGTCTGAATCTTGACGAGGAACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

342

Amino Acids

38.11

Weight (kDa)

5.57

Isoelectric Point (pI)

49.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25805
fragaria_vesca FvH4_4g27661 FvH4_4g27661
malus_domestica MD13G1044100.v1.1
prunus_persica Prupe.1G308400_v2.0.a1
pyrus_communis pycom13g03870
rosa_chinensis RchiOBHm_Chr4g0435511
rosa_laevigata RLG00000006586
rosa_multiflora Rmu_sc0001940.1_g000030
rosa_roxburghii Rroxscaffold_5G00376740
rosa_rugosa Rorug04G0285800
rosa_samantha Rh4BG349700 Rh4CG364400 Rh4DG344000
rosa_wichuraiana Rw4G029790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 977
AccBSI CCGCTC 1 cut(s) 199
AccI GTMKAC 1 cut(s) 303
AccII CGCG 3 cut(s) 54, 210, 212
AclWI GGATC 2 cut(s) 222, 844
AcoI YGGCCR 2 cut(s) 3, 772
AcuI CTGAAG 1 cut(s) 257
AcyI GRCGYC 1 cut(s) 576
AfaI GTAC 1 cut(s) 969
AfeI AGCGCT 1 cut(s) 677
AfiI CCNNNNNNNGG 9 cut(s) 80, 147, 177, 178, 352, 550, 719, 838, 977
AgsI TTSAA 3 cut(s) 66, 193, 493
AjiI CACGTC 1 cut(s) 21
AjnI CCWGG 2 cut(s) 337, 896
AjuI GAANNNNNNNTTGG 2 cut(s) 107, 139
AluBI AGCT 2 cut(s) 685, 710
AluI AGCT 2 cut(s) 685, 710
Alw21I GWGCWC 2 cut(s) 110, 712
Alw26I GTCTC 3 cut(s) 27, 336, 573
Alw44I GTGCAC 1 cut(s) 106
AlwI GGATC 2 cut(s) 222, 844
Aor51HI AGCGCT 1 cut(s) 677
AoxI GGCC 4 cut(s) 3, 71, 149, 772
ApaLI GTGCAC 1 cut(s) 106
ApeKI GCWGC 3 cut(s) 39, 268, 933
Asp700I GAANNNNTTC 1 cut(s) 799
AspA2I CCTAGG 2 cut(s) 79, 832
AspLEI GCGC 5 cut(s) 17, 44, 56, 212, 678
AspS9I GGNCC 3 cut(s) 72, 90, 541
AsuHPI GGTGA 2 cut(s) 461, 920
AvaII GGWCC 2 cut(s) 90, 541
AvrII CCTAGG 2 cut(s) 79, 832
BaeGI GKGCMC 1 cut(s) 110
BanII GRGCYC 3 cut(s) 393, 712, 766
BauI CACGAG 1 cut(s) 200
BbsI GAAGAC 1 cut(s) 880
Bbv12I GWGCWC 2 cut(s) 110, 712
BbvCI CCTCAGC 1 cut(s) 294
BbvI GCAGC 3 cut(s) 26, 255, 945
BccI CCATC 4 cut(s) 722, 772, 776, 804
BceAI ACGGC 3 cut(s) 271, 281, 869
BciT130I CCWGG 2 cut(s) 339, 898
BciVI GTATCC 1 cut(s) 361
BcoDI GTCTC 3 cut(s) 27, 336, 573
BfaI CTAG 4 cut(s) 69, 80, 702, 833
BfmI CTRYAG 1 cut(s) 1022
BfoI RGCGCY 2 cut(s) 18, 679
BfuI GTATCC 1 cut(s) 361
BisI GCNGC 7 cut(s) 40, 52, 129, 197, 208, 269, 934
BlnI CCTAGG 2 cut(s) 79, 832
BlsI GCNGC 7 cut(s) 41, 53, 130, 198, 209, 270, 935
Bme1390I CCNGG 2 cut(s) 339, 898
Bme18I GGWCC 2 cut(s) 90, 541
BmgBI CACGTC 1 cut(s) 21
BmgT120I GGNCC 3 cut(s) 72, 90, 541
BmrFI CCNGG 2 cut(s) 339, 898
BmrI ACTGGG 2 cut(s) 341, 455
BmsI GCATC 2 cut(s) 445, 698
BmuI ACTGGG 2 cut(s) 341, 455
BoxI GACNNNNGTC 1 cut(s) 873
BpiI GAAGAC 1 cut(s) 880
BpmI CTGGAG 1 cut(s) 360
Bpu10I CCTNAGC 1 cut(s) 294
BsaHI GRCGYC 1 cut(s) 576
BsaI GGTCTC 2 cut(s) 336, 573
BsaJI CCNNGG 3 cut(s) 79, 832, 896
BsaXI ACNNNNNCTCC 2 cut(s) 333, 363
Bsc4I CCNNNNNNNGG 9 cut(s) 80, 147, 177, 178, 352, 550, 719, 838, 977
Bse1I ACTGG 4 cut(s) 138, 347, 461, 971
BseBI CCWGG 2 cut(s) 339, 898
BseDI CCNNGG 3 cut(s) 79, 832, 896
BseGI GGATG 2 cut(s) 783, 787
BseLI CCNNNNNNNGG 9 cut(s) 80, 147, 177, 178, 352, 550, 719, 838, 977
BseMII CTCAG 4 cut(s) 273, 285, 471, 642
BseNI ACTGG 4 cut(s) 138, 347, 461, 971
BseRI GAGGAG 1 cut(s) 516
BseSI GKGCMC 1 cut(s) 110
BseXI GCAGC 3 cut(s) 26, 255, 945
Bsh1236I CGCG 3 cut(s) 54, 210, 212
Bsh1285I CGRYCG 1 cut(s) 49
BshFI GGCC 4 cut(s) 5, 73, 151, 774
BsiEI CGRYCG 1 cut(s) 49
BsiHKAI GWGCWC 2 cut(s) 110, 712
BsiSI CCGG 1 cut(s) 775
BslFI GGGAC 3 cut(s) 194, 566, 881
BslI CCNNNNNNNGG 9 cut(s) 80, 147, 177, 178, 352, 550, 719, 838, 977
BsmAI GTCTC 3 cut(s) 27, 336, 573
BsmBI CGTCTC 1 cut(s) 27
BsmFI GGGAC 3 cut(s) 194, 566, 881
BsmI GAATGC 1 cut(s) 857
BsnI GGCC 4 cut(s) 5, 73, 151, 774
Bso31I GGTCTC 2 cut(s) 336, 573
Bsp1286I GDGCHC 4 cut(s) 110, 393, 712, 766
Bsp143I GATC 3 cut(s) 214, 822, 836
BspANI GGCC 4 cut(s) 5, 73, 151, 774
BspCNI CTCAG 4 cut(s) 272, 286, 472, 643
BspFNI CGCG 3 cut(s) 54, 210, 212
BspPI GGATC 2 cut(s) 222, 844
BspTNI GGTCTC 2 cut(s) 336, 573
BsrBI CCGCTC 1 cut(s) 199
BsrI ACTGG 4 cut(s) 138, 347, 461, 971
BssECI CCNNGG 3 cut(s) 79, 832, 896
BssMI GATC 3 cut(s) 214, 822, 836
BssNI GRCGYC 1 cut(s) 576
BssSI CACGAG 1 cut(s) 200
BssT1I CCWWGG 2 cut(s) 79, 832
Bst2BI CACGAG 1 cut(s) 200
Bst2UI CCWGG 2 cut(s) 339, 898
Bst4CI ACNGT 2 cut(s) 373, 1023
BstACI GRCGYC 1 cut(s) 576
BstC8I GCNNGC 5 cut(s) 165, 291, 674, 687, 849
BstDEI CTNAG 6 cut(s) 259, 294, 480, 651, 959, 982
BstEII GGTNACC 1 cut(s) 827
BstF5I GGATG 2 cut(s) 783, 787
BstFNI CGCG 3 cut(s) 54, 210, 212
BstH2I RGCGCY 2 cut(s) 18, 679
BstHHI GCGC 5 cut(s) 17, 44, 56, 212, 678
BstKTI GATC 3 cut(s) 217, 825, 839
BstMAI GTCTC 3 cut(s) 27, 336, 573
BstMBI GATC 3 cut(s) 214, 822, 836
BstMCI CGRYCG 1 cut(s) 49
BstMWI GCNNNNNNNGC 5 cut(s) 48, 216, 682, 734, 761
BstNI CCWGG 2 cut(s) 339, 898
BstPAI GACNNNNGTC 1 cut(s) 873
BstPI GGTNACC 1 cut(s) 827
BstSCI CCNGG 2 cut(s) 337, 896
BstSFI CTRYAG 1 cut(s) 1022
BstSLI GKGCMC 1 cut(s) 110
BstUI CGCG 3 cut(s) 54, 210, 212
BstV1I GCAGC 3 cut(s) 26, 255, 945
BstV2I GAAGAC 1 cut(s) 880
BsuI GTATCC 1 cut(s) 361
BsuRI GGCC 4 cut(s) 5, 73, 151, 774
BtrI CACGTC 1 cut(s) 21
BtsCI GGATG 2 cut(s) 783, 787
BtsIMutI CAGTG 4 cut(s) 131, 354, 378, 699
Cac8I GCNNGC 5 cut(s) 165, 291, 674, 687, 849
CfoI GCGC 5 cut(s) 17, 44, 56, 212, 678
Cfr13I GGNCC 3 cut(s) 72, 90, 541
CpoI CGGWCCG 1 cut(s) 541
CseI GACGC 1 cut(s) 584
Csp6I GTAC 1 cut(s) 968
CspI CGGWCCG 1 cut(s) 541
CviAII CATG 2 cut(s) 517, 628
CviQI GTAC 1 cut(s) 968
DdeI CTNAG 6 cut(s) 259, 294, 480, 651, 959, 982
DpnI GATC 3 cut(s) 216, 824, 838
DpnII GATC 3 cut(s) 214, 822, 836
EaeI YGGCCR 2 cut(s) 3, 772
EciI GGCGGA 1 cut(s) 15
Ecl136II GAGCTC 1 cut(s) 710
Eco130I CCWWGG 2 cut(s) 79, 832
Eco24I GRGCYC 3 cut(s) 393, 712, 766
Eco31I GGTCTC 2 cut(s) 336, 573
Eco47I GGWCC 2 cut(s) 90, 541
Eco47III AGCGCT 1 cut(s) 677
Eco53kI GAGCTC 1 cut(s) 710
Eco57I CTGAAG 1 cut(s) 257
Eco91I GGTNACC 1 cut(s) 827
EcoICRI GAGCTC 1 cut(s) 710
EcoO109I RGGNCCY 1 cut(s) 72
EcoO65I GGTNACC 1 cut(s) 827
EcoRII CCWGG 2 cut(s) 337, 896
EcoT14I CCWWGG 2 cut(s) 79, 832
EcoT22I ATGCAT 1 cut(s) 859
EcoT38I GRGCYC 3 cut(s) 393, 712, 766
ErhI CCWWGG 2 cut(s) 79, 832
Esp3I CGTCTC 1 cut(s) 27
FaeI CATG 2 cut(s) 520, 631
FaiI YATR 6 cut(s) 333, 518, 629, 742, 760, 855
FaqI GGGAC 3 cut(s) 194, 566, 881
FatI CATG 2 cut(s) 516, 627
FauI CCCGC 1 cut(s) 172
FblI GTMKAC 1 cut(s) 303
Fnu4HI GCNGC 7 cut(s) 40, 52, 129, 197, 208, 269, 934
FokI GGATG 2 cut(s) 790, 794
FriOI GRGCYC 3 cut(s) 393, 712, 766
Fsp4HI GCNGC 7 cut(s) 40, 52, 129, 197, 208, 269, 934
FspBI CTAG 4 cut(s) 69, 80, 702, 833
GlaI GCGC 5 cut(s) 16, 43, 55, 211, 677
GluI GCNGC 7 cut(s) 40, 52, 129, 197, 208, 269, 934
GsuI CTGGAG 1 cut(s) 360
HaeII RGCGCY 2 cut(s) 18, 679
HaeIII GGCC 4 cut(s) 5, 73, 151, 774
HapII CCGG 1 cut(s) 775
HgaI GACGC 1 cut(s) 584
HhaI GCGC 5 cut(s) 17, 44, 56, 212, 678
Hin1I GRCGYC 1 cut(s) 576
Hin1II CATG 2 cut(s) 520, 631
Hin6I GCGC 5 cut(s) 15, 42, 54, 210, 676
HinP1I GCGC 5 cut(s) 15, 42, 54, 210, 676
HinfI GANTC 6 cut(s) 115, 137, 182, 300, 653, 1006
HpaII CCGG 1 cut(s) 775
HphI GGTGA 2 cut(s) 461, 920
Hpy166II GTNNAC 5 cut(s) 108, 304, 376, 510, 537
Hpy188III TCNNGA 3 cut(s) 572, 820, 1010
Hpy8I GTNNAC 5 cut(s) 108, 304, 376, 510, 537
Hpy99I CGWCG 3 cut(s) 11, 257, 578
HpyAV CCTTC 2 cut(s) 162, 540
HpyCH4III ACNGT 2 cut(s) 373, 1023
HpyCH4IV ACGT 2 cut(s) 20, 146
HpyCH4V TGCA 5 cut(s) 108, 689, 755, 857, 919
HpyF10VI GCNNNNNNNGC 5 cut(s) 48, 216, 682, 734, 761
HpyF3I CTNAG 6 cut(s) 259, 294, 480, 651, 959, 982
HpySE526I ACGT 2 cut(s) 20, 146
Hsp92I GRCGYC 1 cut(s) 576
Hsp92II CATG 2 cut(s) 520, 631
HspAI GCGC 5 cut(s) 15, 42, 54, 210, 676
Kzo9I GATC 3 cut(s) 214, 822, 836
LmnI GCTCC 5 cut(s) 340, 529, 707, 715, 734
Lsp1109I GCAGC 3 cut(s) 26, 255, 945
LweI GCATC 2 cut(s) 445, 698
MaeI CTAG 4 cut(s) 69, 80, 702, 833
MaeII ACGT 2 cut(s) 20, 146
MaeIII GTNAC 2 cut(s) 827, 908
MalI GATC 3 cut(s) 216, 824, 838
MbiI CCGCTC 1 cut(s) 199
MboI GATC 3 cut(s) 214, 822, 836
MboII GAAGA 6 cut(s) 314, 317, 397, 400, 458, 880
MfeI CAATTG 1 cut(s) 101
MhlI GDGCHC 4 cut(s) 110, 393, 712, 766
MluCI AATT 4 cut(s) 101, 188, 400, 952
MlyI GAGTC 4 cut(s) 131, 176, 309, 662
MmeI TCCRAC 1 cut(s) 700
Mph1103I ATGCAT 1 cut(s) 859
MroXI GAANNNNTTC 1 cut(s) 799
MseI TTAA 1 cut(s) 399
MspI CCGG 1 cut(s) 775
MspR9I CCNGG 2 cut(s) 339, 898
MunI CAATTG 1 cut(s) 101
Mva1269I GAATGC 1 cut(s) 857
MvaI CCWGG 2 cut(s) 339, 898
MvnI CGCG 3 cut(s) 54, 210, 212
MwoI GCNNNNNNNGC 5 cut(s) 48, 216, 682, 734, 761
NdeII GATC 3 cut(s) 214, 822, 836
NlaIII CATG 2 cut(s) 520, 631
NmuCI GTSAC 1 cut(s) 908
NsiI ATGCAT 1 cut(s) 859
PctI GAATGC 1 cut(s) 857
PdmI GAANNNNTTC 1 cut(s) 799
PfeI GAWTC 2 cut(s) 115, 1006
PflMI CCANNNNNTGG 1 cut(s) 977
PfoI TCCNGGA 1 cut(s) 337
PkrI GCNGC 7 cut(s) 41, 53, 130, 198, 209, 270, 935
PleI GAGTC 4 cut(s) 131, 176, 308, 661
PpsI GAGTC 4 cut(s) 131, 176, 308, 661
PshAI GACNNNNGTC 1 cut(s) 873
Psp124BI GAGCTC 1 cut(s) 712
Psp6I CCWGG 2 cut(s) 337, 896
PspEI GGTNACC 1 cut(s) 827
PspGI CCWGG 2 cut(s) 337, 896
PspPI GGNCC 3 cut(s) 72, 90, 541
RsaI GTAC 1 cut(s) 969
RsaNI GTAC 1 cut(s) 968
Rsr2I CGGWCCG 1 cut(s) 541
RsrII CGGWCCG 1 cut(s) 541
SacI GAGCTC 1 cut(s) 712
SaqAI TTAA 1 cut(s) 399
SatI GCNGC 7 cut(s) 40, 52, 129, 197, 208, 269, 934
Sau3AI GATC 3 cut(s) 214, 822, 836
Sau96I GGNCC 3 cut(s) 72, 90, 541
SchI GAGTC 4 cut(s) 131, 176, 309, 662
ScrFI CCNGG 2 cut(s) 339, 898
SduI GDGCHC 4 cut(s) 110, 393, 712, 766
SetI ASST 9 cut(s) 23, 149, 236, 625, 687, 712, 834, 910, 996
SfaNI GCATC 2 cut(s) 445, 698
SfcI CTRYAG 1 cut(s) 1022
SinI GGWCC 2 cut(s) 90, 541
Sse9I AATT 4 cut(s) 101, 188, 400, 952
SspMI CTAG 4 cut(s) 69, 80, 702, 833
SstI GAGCTC 1 cut(s) 712
StyD4I CCNGG 2 cut(s) 337, 896
StyI CCWWGG 2 cut(s) 79, 832
TaaI ACNGT 2 cut(s) 373, 1023
TaiI ACGT 2 cut(s) 23, 149
TaqI TCGA 1 cut(s) 573
TaqII GACCGA 2 cut(s) 558, 822
TasI AATT 4 cut(s) 101, 188, 400, 952
TauI GCSGC 4 cut(s) 54, 131, 199, 210
TfiI GAWTC 2 cut(s) 115, 1006
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TscAI CASTG 4 cut(s) 138, 354, 378, 699
TseFI GTSAC 1 cut(s) 908
TseI GCWGC 3 cut(s) 39, 268, 933
Tsp45I GTSAC 1 cut(s) 908
TspDTI ATGAA 3 cut(s) 400, 505, 850
TspGWI ACGGA 3 cut(s) 226, 421, 554
TspRI CASTG 4 cut(s) 138, 354, 378, 699
Van91I CCANNNNNTGG 1 cut(s) 977
VneI GTGCAC 1 cut(s) 106
VpaK11BI GGWCC 2 cut(s) 90, 541
XmaJI CCTAGG 2 cut(s) 79, 832
XmiI GTMKAC 1 cut(s) 303
XmnI GAANNNNTTC 1 cut(s) 799
XspI CTAG 4 cut(s) 69, 80, 702, 833
Zsp2I ATGCAT 1 cut(s) 859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.