FvH4_4g27720

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016636
Physical Location & Seq
Reverse (-)
28586626 .. 28587341
716 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27720.t1

Sequence Viewer

Length: 537 bp
ATGGACGAATTGCAGAACGAGTCTCTCCCTTCGGCCCCATCGGTGCCGCCACTAAGTCCTCGGATTAAACCATCATCAGCACCACCCGTTCCTTTTGATTTGAATGATCAGCCCCACACAACCACCGGCGTTCCGGTCGTGCCGGTGACCCTTCCTCATTCCCCAGCCCCTTGGTCCAGTGGCCTATGCGATTGTTGTGAAGATCTCAGTAGTTGTAAGTCCGATCATTTGTTGCTTAACATGTTGGTGTCCATGCATAACATTTGGACGCATCGCAGAAATTGTGGACAGGGGATCAACTTCCTGTGGAGTGAGTGGAACACTATACGGTTTGATGATGTGCTTGATGGGTTGCTCGTGTTTGTATTCGTGCTTCTATCGAACCAGACTGAGAGGGCAGTACTTCTTGGAGGAGAAGCCATGCGCAGATTGCTGCGTCCATTTTTGCTGCGAGGAATGCGCTCTCTGCCAAGAGTATCGACACCTTCAGAACCAGGGCCTCGACATGACCATAGGTTGGTATGGCAACGTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

179

Amino Acids

19.97

Weight (kDa)

6.64

Isoelectric Point (pI)

70.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016636)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68610
fragaria_vesca FvH4_4g27720
malus_domestica MD16G1045800.v1.1
prunus_persica Prupe.1G307600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0435581
rosa_laevigata RLG00000006580
rosa_multiflora Rmu_sc0006697.1_g000003
rosa_roxburghii Rroxscaffold_5G00376810
rosa_rugosa Rorug04G0286800.1
rosa_samantha Rh4AG342200 Rh4BG350400 Rh4CG365100 Rh4DG344700
rosa_wichuraiana Rw4G029850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 425
AccB1I GGYRCC 1 cut(s) 43
AccB7I CCANNNNNTGG 1 cut(s) 517
AciI CCGC 1 cut(s) 47
AclWI GGATC 1 cut(s) 302
AcuI CTGAAG 1 cut(s) 471
AfaI GTAC 1 cut(s) 402
AfiI CCNNNNNNNGG 1 cut(s) 517
AflIII ACRYGT 1 cut(s) 240
AgsI TTSAA 1 cut(s) 103
AjnI CCWGG 1 cut(s) 493
Alw26I GTCTC 1 cut(s) 27
AlwI GGATC 1 cut(s) 302
AoxI GGCC 3 cut(s) 33, 181, 497
ApeKI GCWGC 2 cut(s) 433, 448
AspLEI GCGC 2 cut(s) 426, 462
AspS9I GGNCC 3 cut(s) 34, 174, 497
AsuHPI GGTGA 1 cut(s) 157
AvaII GGWCC 1 cut(s) 174
BanI GGYRCC 1 cut(s) 43
BarI GAAGNNNNNNTAC 2 cut(s) 357, 389
BauI CACGAG 1 cut(s) 356
BbvI GCAGC 2 cut(s) 420, 435
BccI CCATC 3 cut(s) 46, 79, 341
BciT130I CCWGG 1 cut(s) 495
BclI TGATCA 1 cut(s) 106
BcoDI GTCTC 1 cut(s) 27
BglII AGATCT 1 cut(s) 202
BisI GCNGC 3 cut(s) 47, 434, 449
BlsI GCNGC 3 cut(s) 48, 435, 450
BmcAI AGTACT 1 cut(s) 402
Bme1390I CCNGG 1 cut(s) 495
Bme18I GGWCC 1 cut(s) 174
BmgT120I GGNCC 3 cut(s) 34, 174, 497
BmiI GGNNCC 2 cut(s) 36, 45
BmrFI CCNGG 1 cut(s) 495
BmsI GCATC 1 cut(s) 280
BsaJI CCNNGG 3 cut(s) 59, 170, 494
BsaWI WCCGGW 1 cut(s) 133
Bsc4I CCNNNNNNNGG 1 cut(s) 517
Bse118I RCCGGY 2 cut(s) 125, 142
Bse1I ACTGG 1 cut(s) 177
BseBI CCWGG 1 cut(s) 495
BseDI CCNNGG 3 cut(s) 59, 170, 494
BseLI CCNNNNNNNGG 1 cut(s) 517
BseMII CTCAG 2 cut(s) 220, 381
BseNI ACTGG 1 cut(s) 177
BseRI GAGGAG 1 cut(s) 426
BseXI GCAGC 2 cut(s) 420, 435
BseYI CCCAGC 1 cut(s) 163
Bsh1285I CGRYCG 1 cut(s) 138
BshFI GGCC 3 cut(s) 35, 183, 499
BshNI GGYRCC 1 cut(s) 43
BsiEI CGRYCG 1 cut(s) 138
BsiSI CCGG 3 cut(s) 126, 134, 143
BslI CCNNNNNNNGG 1 cut(s) 517
BsmAI GTCTC 1 cut(s) 27
BsmI GAATGC 1 cut(s) 462
BsnI GGCC 3 cut(s) 35, 183, 499
Bsp143I GATC 4 cut(s) 106, 202, 223, 294
BspACI CCGC 1 cut(s) 47
BspANI GGCC 3 cut(s) 35, 183, 499
BspCNI CTCAG 2 cut(s) 219, 382
BspLI GGNNCC 2 cut(s) 36, 45
BspPI GGATC 1 cut(s) 302
BspT107I GGYRCC 1 cut(s) 43
BsrFI RCCGGY 2 cut(s) 125, 142
BsrI ACTGG 1 cut(s) 177
BssAI RCCGGY 2 cut(s) 125, 142
BssECI CCNNGG 3 cut(s) 59, 170, 494
BssMI GATC 4 cut(s) 106, 202, 223, 294
BssSI CACGAG 1 cut(s) 356
BssT1I CCWWGG 1 cut(s) 170
Bst2BI CACGAG 1 cut(s) 356
Bst2UI CCWGG 1 cut(s) 495
Bst4CI ACNGT 1 cut(s) 330
BstDEI CTNAG 3 cut(s) 53, 206, 390
BstEII GGTNACC 1 cut(s) 145
BstHHI GCGC 2 cut(s) 426, 462
BstKTI GATC 4 cut(s) 109, 205, 226, 297
BstMAI GTCTC 1 cut(s) 27
BstMBI GATC 4 cut(s) 106, 202, 223, 294
BstMCI CGRYCG 1 cut(s) 138
BstMWI GCNNNNNNNGC 3 cut(s) 430, 457, 466
BstNI CCWGG 1 cut(s) 495
BstNSI RCATGY 1 cut(s) 244
BstPI GGTNACC 1 cut(s) 145
BstSCI CCNGG 1 cut(s) 493
BstV1I GCAGC 2 cut(s) 420, 435
BstX2I RGATCY 1 cut(s) 202
BstXI CCANNNNNNTGG 1 cut(s) 171
BstYI RGATCY 1 cut(s) 202
BsuRI GGCC 3 cut(s) 35, 183, 499
BtgZI GCGATG 1 cut(s) 257
BtsIMutI CAGTG 1 cut(s) 184
CfoI GCGC 2 cut(s) 426, 462
Cfr10I RCCGGY 2 cut(s) 125, 142
Cfr13I GGNCC 3 cut(s) 34, 174, 497
CseI GACGC 2 cut(s) 277, 425
Csp6I GTAC 1 cut(s) 401
CviAII CATG 4 cut(s) 241, 253, 421, 506
CviJI RGCY 6 cut(s) 35, 112, 167, 183, 419, 499
CviKI_1 RGCY 6 cut(s) 35, 112, 167, 183, 419, 499
CviQI GTAC 1 cut(s) 401
DdeI CTNAG 3 cut(s) 53, 206, 390
DpnI GATC 4 cut(s) 108, 204, 225, 296
DpnII GATC 4 cut(s) 106, 202, 223, 294
Eco130I CCWWGG 1 cut(s) 170
Eco47I GGWCC 1 cut(s) 174
Eco57I CTGAAG 1 cut(s) 471
Eco91I GGTNACC 1 cut(s) 145
EcoO109I RGGNCCY 1 cut(s) 497
EcoO65I GGTNACC 1 cut(s) 145
EcoRII CCWGG 1 cut(s) 493
EcoT14I CCWWGG 1 cut(s) 170
EcoT22I ATGCAT 1 cut(s) 258
ErhI CCWWGG 1 cut(s) 170
FaeI CATG 4 cut(s) 244, 256, 424, 509
FatI CATG 4 cut(s) 240, 252, 420, 505
FbaI TGATCA 1 cut(s) 106
Fnu4HI GCNGC 3 cut(s) 47, 434, 449
Fsp4HI GCNGC 3 cut(s) 47, 434, 449
FspI TGCGCA 1 cut(s) 425
GlaI GCGC 2 cut(s) 425, 461
GluI GCNGC 3 cut(s) 47, 434, 449
GsaI CCCAGC 1 cut(s) 167
HaeIII GGCC 3 cut(s) 35, 183, 499
HapII CCGG 3 cut(s) 126, 134, 143
HgaI GACGC 2 cut(s) 277, 425
HhaI GCGC 2 cut(s) 426, 462
Hin1II CATG 4 cut(s) 244, 256, 424, 509
Hin6I GCGC 2 cut(s) 424, 460
HinP1I GCGC 2 cut(s) 424, 460
HinfI GANTC 1 cut(s) 20
HpaII CCGG 3 cut(s) 126, 134, 143
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 1 cut(s) 287
Hpy188I TCNGA 3 cut(s) 63, 223, 490
Hpy8I GTNNAC 1 cut(s) 287
HpyAV CCTTC 3 cut(s) 39, 161, 495
HpyCH4III ACNGT 1 cut(s) 330
HpyCH4IV ACGT 1 cut(s) 529
HpyCH4V TGCA 2 cut(s) 13, 256
HpyF10VI GCNNNNNNNGC 3 cut(s) 430, 457, 466
HpyF3I CTNAG 3 cut(s) 53, 206, 390
HpySE526I ACGT 1 cut(s) 529
Hsp92II CATG 4 cut(s) 244, 256, 424, 509
HspAI GCGC 2 cut(s) 424, 460
Ksp22I TGATCA 1 cut(s) 106
Kzo9I GATC 4 cut(s) 106, 202, 223, 294
Lsp1109I GCAGC 2 cut(s) 420, 435
LweI GCATC 1 cut(s) 280
MaeII ACGT 1 cut(s) 529
MaeIII GTNAC 1 cut(s) 145
MalI GATC 4 cut(s) 108, 204, 225, 296
MboI GATC 4 cut(s) 106, 202, 223, 294
MboII GAAGA 1 cut(s) 212
MflI RGATCY 1 cut(s) 202
MluCI AATT 2 cut(s) 8, 280
MlyI GAGTC 1 cut(s) 29
MnlI CCTC 6 cut(s) 69, 165, 387, 404, 446, 510
Mph1103I ATGCAT 1 cut(s) 258
MseI TTAA 2 cut(s) 66, 237
MslI CAYNNNNRTG 1 cut(s) 245
MspI CCGG 3 cut(s) 126, 134, 143
MspR9I CCNGG 1 cut(s) 495
Mva1269I GAATGC 1 cut(s) 462
MvaI CCWGG 1 cut(s) 495
MwoI GCNNNNNNNGC 3 cut(s) 430, 457, 466
NdeII GATC 4 cut(s) 106, 202, 223, 294
NlaIII CATG 4 cut(s) 244, 256, 424, 509
NlaIV GGNNCC 2 cut(s) 36, 45
NmuCI GTSAC 1 cut(s) 145
NsbI TGCGCA 1 cut(s) 425
NsiI ATGCAT 1 cut(s) 258
NspI RCATGY 1 cut(s) 244
PciI ACATGT 1 cut(s) 240
PctI GAATGC 1 cut(s) 462
PflMI CCANNNNNTGG 1 cut(s) 517
PkrI GCNGC 3 cut(s) 48, 435, 450
PleI GAGTC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 28
PscI ACATGT 1 cut(s) 240
Psp6I CCWGG 1 cut(s) 493
PspEI GGTNACC 1 cut(s) 145
PspFI CCCAGC 1 cut(s) 163
PspGI CCWGG 1 cut(s) 493
PspN4I GGNNCC 2 cut(s) 36, 45
PspPI GGNCC 3 cut(s) 34, 174, 497
PsuI RGATCY 1 cut(s) 202
RsaI GTAC 1 cut(s) 402
RsaNI GTAC 1 cut(s) 401
RseI CAYNNNNRTG 1 cut(s) 245
SaqAI TTAA 2 cut(s) 66, 237
SatI GCNGC 3 cut(s) 47, 434, 449
Sau3AI GATC 4 cut(s) 106, 202, 223, 294
Sau96I GGNCC 3 cut(s) 34, 174, 497
ScaI AGTACT 1 cut(s) 402
SchI GAGTC 1 cut(s) 29
ScrFI CCNGG 1 cut(s) 495
SetI ASST 3 cut(s) 487, 518, 532
SfaNI GCATC 1 cut(s) 280
SgrAI CRCCGGYG 1 cut(s) 125
SinI GGWCC 1 cut(s) 174
SmiMI CAYNNNNRTG 1 cut(s) 245
Sse9I AATT 2 cut(s) 8, 280
SsiI CCGC 1 cut(s) 47
StyD4I CCNGG 1 cut(s) 493
StyI CCWWGG 1 cut(s) 170
TaaI ACNGT 1 cut(s) 330
TaiI ACGT 1 cut(s) 532
TaqI TCGA 3 cut(s) 380, 479, 502
TasI AATT 2 cut(s) 8, 280
TatI WGTACW 1 cut(s) 400
TauI GCSGC 1 cut(s) 49
Tru1I TTAA 2 cut(s) 66, 237
Tru9I TTAA 2 cut(s) 66, 237
TscAI CASTG 1 cut(s) 184
TseFI GTSAC 1 cut(s) 145
TseI GCWGC 2 cut(s) 433, 448
Tsp45I GTSAC 1 cut(s) 145
TspRI CASTG 1 cut(s) 184
Van91I CCANNNNNTGG 1 cut(s) 517
VpaK11BI GGWCC 1 cut(s) 174
XceI RCATGY 1 cut(s) 244
ZrmI AGTACT 1 cut(s) 402
Zsp2I ATGCAT 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.