MD16G1045800.v1.1

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0016636
Physical Location & Seq
Reverse (-)
3197084 .. 3198039
956 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1045800.v1.1.491

Sequence Viewer

Length: 543 bp
ATGGGCATGGCTAAGTTAGAAAGAGAGTCAATGCGGCCGCCATCCCCATCAGTGCCGCCTCTAACAACCACTGGCATTCCGCTTAACCTAATTAGCACCTGTACTTCTGCTTCCCCGATGGCTTGGTCCACCGGGCTCTGCAATTGTTGTGGTGATGTTAGCAGTTGTTGCTTGACGTGTTGGTTTCCATGTGTAACATTTGGACGCATTGCAGAAATTGTGGATAGGGGATCAACTCCTTGTGGAGTGAGTGGAACTCTTTACAGTTTGATGATGTGCTTGATGGGTTGCTCGTGTTTGTACTCGTGCTTCTATAGAACCAAATTGAGAGGACAATATTTCTTGGAGGAGAAGCCCTGCACGGATTGCTGCGTCCATTTTTGCTGCGAGGCATGTGCTTTGTGCCAAGAGTATAGACATCTTCAGAACCAGGGCTTTGACATGTCCATCGGCTGGCACGGCAACGTTGAAAGGCAGAGACGACTGGCTCCAACATCAATGCCACTACATCCACAACTTTATGCAGCAGGCATGACGCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

181

Amino Acids

19.88

Weight (kDa)

7.79

Isoelectric Point (pI)

52.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 42 - 140 9.1e-33 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016636)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68610
fragaria_vesca FvH4_4g27720
malus_domestica MD16G1045800.v1.1
prunus_persica Prupe.1G307600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0435581
rosa_laevigata RLG00000006580
rosa_multiflora Rmu_sc0006697.1_g000003
rosa_roxburghii Rroxscaffold_5G00376810
rosa_rugosa Rorug04G0286800.1
rosa_samantha Rh4AG342200 Rh4BG350400 Rh4CG365100 Rh4DG344700
rosa_wichuraiana Rw4G029850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 453
AccII CGCG 1 cut(s) 538
AciI CCGC 4 cut(s) 34, 38, 56, 80
AclI AACGTT 1 cut(s) 465
AclWI GGATC 1 cut(s) 238
AcoI YGGCCR 1 cut(s) 35
AcuI CTGAAG 1 cut(s) 407
AfaI GTAC 2 cut(s) 103, 302
AfiI CCNNNNNNNGG 1 cut(s) 453
AflIII ACRYGT 2 cut(s) 176, 441
AgsI TTSAA 1 cut(s) 470
AjiI CACGTC 1 cut(s) 177
AjnI CCWGG 1 cut(s) 429
Alw26I GTCTC 1 cut(s) 472
AlwI GGATC 1 cut(s) 238
AoxI GGCC 1 cut(s) 35
ApeKI GCWGC 3 cut(s) 369, 384, 524
AspLEI GCGC 1 cut(s) 540
AspS9I GGNCC 1 cut(s) 126
AsuC2I CCSGG 1 cut(s) 133
AsuHPI GGTGA 1 cut(s) 164
AvaII GGWCC 1 cut(s) 126
BanII GRGCYC 1 cut(s) 138
BarI GAAGNNNNNNTAC 4 cut(s) 94, 126, 293, 325
BauI CACGAG 2 cut(s) 292, 304
BbvI GCAGC 3 cut(s) 356, 371, 536
BccI CCATC 5 cut(s) 49, 55, 112, 277, 455
BceAI ACGGC 1 cut(s) 475
BcgI CGANNNNNNTGC 2 cut(s) 377, 411
BciT130I CCWGG 1 cut(s) 431
BcnI CCSGG 1 cut(s) 133
BcoDI GTCTC 1 cut(s) 472
BfaI CTAG 1 cut(s) 541
BfmI CTRYAG 1 cut(s) 313
BisI GCNGC 6 cut(s) 35, 38, 56, 370, 385, 525
BlsI GCNGC 6 cut(s) 36, 39, 57, 371, 386, 526
Bme1390I CCNGG 2 cut(s) 133, 431
Bme18I GGWCC 1 cut(s) 126
BmgBI CACGTC 1 cut(s) 177
BmgT120I GGNCC 1 cut(s) 126
BmiI GGNNCC 1 cut(s) 489
BmrFI CCNGG 2 cut(s) 133, 431
BplI GAGNNNNNCTC 2 cut(s) 241, 273
BpuMI CCSGG 1 cut(s) 133
BsaJI CCNNGG 1 cut(s) 430
Bsc4I CCNNNNNNNGG 1 cut(s) 453
Bse1I ACTGG 2 cut(s) 76, 489
Bse3DI GCAATG 1 cut(s) 207
BseBI CCWGG 1 cut(s) 431
BseDI CCNNGG 1 cut(s) 430
BseGI GGATG 2 cut(s) 41, 508
BseLI CCNNNNNNNGG 1 cut(s) 453
BseMI GCAATG 1 cut(s) 207
BseNI ACTGG 2 cut(s) 76, 489
BseRI GAGGAG 1 cut(s) 362
BseX3I CGGCCG 1 cut(s) 35
BseXI GCAGC 3 cut(s) 356, 371, 536
BsgI GTGCAG 1 cut(s) 343
Bsh1236I CGCG 1 cut(s) 538
Bsh1285I CGRYCG 1 cut(s) 38
BshFI GGCC 1 cut(s) 37
BsiEI CGRYCG 1 cut(s) 38
BsiSI CCGG 1 cut(s) 132
BslI CCNNNNNNNGG 1 cut(s) 453
BsmAI GTCTC 1 cut(s) 472
BsmBI CGTCTC 1 cut(s) 472
BsmI GAATGC 1 cut(s) 75
BsnI GGCC 1 cut(s) 37
Bsp1286I GDGCHC 1 cut(s) 138
Bsp143I GATC 1 cut(s) 230
BspACI CCGC 4 cut(s) 34, 38, 56, 80
BspANI GGCC 1 cut(s) 37
BspFNI CGCG 1 cut(s) 538
BspLI GGNNCC 1 cut(s) 489
BspPI GGATC 1 cut(s) 238
BsrDI GCAATG 1 cut(s) 207
BsrI ACTGG 2 cut(s) 76, 489
BssECI CCNNGG 1 cut(s) 430
BssMI GATC 1 cut(s) 230
BssSI CACGAG 2 cut(s) 292, 304
Bst2BI CACGAG 2 cut(s) 292, 304
Bst2UI CCWGG 1 cut(s) 431
Bst4CI ACNGT 1 cut(s) 266
BstAPI GCANNNNNTGC 2 cut(s) 168, 366
BstC8I GCNNGC 2 cut(s) 455, 529
BstDEI CTNAG 1 cut(s) 12
BstF5I GGATG 2 cut(s) 41, 508
BstFNI CGCG 1 cut(s) 538
BstHHI GCGC 1 cut(s) 540
BstKTI GATC 1 cut(s) 233
BstMAI GTCTC 1 cut(s) 472
BstMBI GATC 1 cut(s) 230
BstMCI CGRYCG 1 cut(s) 38
BstMWI GCNNNNNNNGC 4 cut(s) 168, 366, 459, 537
BstNI CCWGG 1 cut(s) 431
BstNSI RCATGY 2 cut(s) 396, 445
BstSCI CCNGG 2 cut(s) 131, 429
BstSFI CTRYAG 1 cut(s) 313
BstUI CGCG 1 cut(s) 538
BstV1I GCAGC 3 cut(s) 356, 371, 536
BstZI CGGCCG 1 cut(s) 35
BsuRI GGCC 1 cut(s) 37
BtrI CACGTC 1 cut(s) 177
BtsCI GGATG 2 cut(s) 41, 508
BtsIMutI CAGTG 2 cut(s) 57, 69
Cac8I GCNNGC 2 cut(s) 455, 529
CciNI GCGGCCGC 1 cut(s) 35
CfoI GCGC 1 cut(s) 540
Cfr13I GGNCC 1 cut(s) 126
CseI GACGC 2 cut(s) 213, 361
Csp6I GTAC 2 cut(s) 102, 301
CspCI CAANNNNNGTGG 2 cut(s) 130, 165
CviAII CATG 5 cut(s) 7, 189, 393, 442, 532
CviJI RGCY 8 cut(s) 11, 37, 122, 136, 355, 435, 453, 488
CviKI_1 RGCY 8 cut(s) 11, 37, 122, 136, 355, 435, 453, 488
CviQI GTAC 2 cut(s) 102, 301
DdeI CTNAG 1 cut(s) 12
DpnI GATC 1 cut(s) 232
DpnII GATC 1 cut(s) 230
EaeI YGGCCR 1 cut(s) 35
EagI CGGCCG 1 cut(s) 35
EclXI CGGCCG 1 cut(s) 35
Eco24I GRGCYC 1 cut(s) 138
Eco47I GGWCC 1 cut(s) 126
Eco52I CGGCCG 1 cut(s) 35
Eco57I CTGAAG 1 cut(s) 407
EcoRII CCWGG 1 cut(s) 429
EcoT38I GRGCYC 1 cut(s) 138
Esp3I CGTCTC 1 cut(s) 472
FaeI CATG 5 cut(s) 10, 192, 396, 445, 535
FaiI YATR 8 cut(s) 8, 190, 315, 394, 414, 443, 522, 533
FatI CATG 5 cut(s) 6, 188, 392, 441, 531
Fnu4HI GCNGC 6 cut(s) 35, 38, 56, 370, 385, 525
FokI GGATG 2 cut(s) 28, 495
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 6 cut(s) 35, 38, 56, 370, 385, 525
FspBI CTAG 1 cut(s) 541
GlaI GCGC 1 cut(s) 539
GluI GCNGC 6 cut(s) 35, 38, 56, 370, 385, 525
HaeIII GGCC 1 cut(s) 37
HapII CCGG 1 cut(s) 132
HgaI GACGC 2 cut(s) 213, 361
HhaI GCGC 1 cut(s) 540
Hin1II CATG 5 cut(s) 10, 192, 396, 445, 535
Hin6I GCGC 1 cut(s) 538
HinP1I GCGC 1 cut(s) 538
HinfI GANTC 1 cut(s) 26
HpaII CCGG 1 cut(s) 132
HphI GGTGA 1 cut(s) 164
Hpy166II GTNNAC 1 cut(s) 129
Hpy188I TCNGA 1 cut(s) 426
Hpy8I GTNNAC 1 cut(s) 129
HpyCH4III ACNGT 1 cut(s) 266
HpyCH4IV ACGT 2 cut(s) 176, 465
HpyCH4V TGCA 4 cut(s) 141, 212, 360, 524
HpyF10VI GCNNNNNNNGC 4 cut(s) 168, 366, 459, 537
HpyF3I CTNAG 1 cut(s) 12
HpySE526I ACGT 2 cut(s) 176, 465
Hsp92II CATG 5 cut(s) 10, 192, 396, 445, 535
HspAI GCGC 1 cut(s) 538
Kzo9I GATC 1 cut(s) 230
LmnI GCTCC 1 cut(s) 493
LpnPI CCDG 9 cut(s) 57, 112, 145, 370, 416, 439, 443, 470, 513
Lsp1109I GCAGC 3 cut(s) 356, 371, 536
MaeI CTAG 1 cut(s) 541
MaeII ACGT 2 cut(s) 176, 465
MaeIII GTNAC 1 cut(s) 193
MalI GATC 1 cut(s) 232
MboI GATC 1 cut(s) 230
MboII GAAGA 1 cut(s) 413
MfeI CAATTG 1 cut(s) 142
MhlI GDGCHC 1 cut(s) 138
MluCI AATT 4 cut(s) 90, 142, 216, 323
MlyI GAGTC 1 cut(s) 35
MmeI TCCRAC 1 cut(s) 515
MnlI CCTC 4 cut(s) 69, 323, 340, 382
MseI TTAA 1 cut(s) 84
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 2 cut(s) 133, 431
MunI CAATTG 1 cut(s) 142
Mva1269I GAATGC 1 cut(s) 75
MvaI CCWGG 1 cut(s) 431
MvnI CGCG 1 cut(s) 538
MwoI GCNNNNNNNGC 4 cut(s) 168, 366, 459, 537
NciI CCSGG 1 cut(s) 133
NdeII GATC 1 cut(s) 230
NlaIII CATG 5 cut(s) 10, 192, 396, 445, 535
NlaIV GGNNCC 1 cut(s) 489
NotI GCGGCCGC 1 cut(s) 35
NspI RCATGY 2 cut(s) 396, 445
PciI ACATGT 1 cut(s) 441
PctI GAATGC 1 cut(s) 75
PflMI CCANNNNNTGG 1 cut(s) 453
PkrI GCNGC 6 cut(s) 36, 39, 57, 371, 386, 526
PleI GAGTC 1 cut(s) 34
PpsI GAGTC 1 cut(s) 34
PscI ACATGT 1 cut(s) 441
Psp1406I AACGTT 1 cut(s) 465
Psp6I CCWGG 1 cut(s) 429
PspGI CCWGG 1 cut(s) 429
PspN4I GGNNCC 1 cut(s) 489
PspPI GGNCC 1 cut(s) 126
RsaI GTAC 2 cut(s) 103, 302
RsaNI GTAC 2 cut(s) 102, 301
SaqAI TTAA 1 cut(s) 84
SatI GCNGC 6 cut(s) 35, 38, 56, 370, 385, 525
Sau3AI GATC 1 cut(s) 230
Sau96I GGNCC 1 cut(s) 126
SchI GAGTC 1 cut(s) 35
ScrFI CCNGG 2 cut(s) 133, 431
SduI GDGCHC 1 cut(s) 138
SetI ASST 4 cut(s) 90, 101, 179, 468
SfcI CTRYAG 1 cut(s) 313
SinI GGWCC 1 cut(s) 126
Sse9I AATT 4 cut(s) 90, 142, 216, 323
SsiI CCGC 4 cut(s) 34, 38, 56, 80
SspI AATATT 1 cut(s) 338
SspMI CTAG 1 cut(s) 541
StyD4I CCNGG 2 cut(s) 131, 429
TaaI ACNGT 1 cut(s) 266
TaiI ACGT 2 cut(s) 179, 468
TasI AATT 4 cut(s) 90, 142, 216, 323
TatI WGTACW 2 cut(s) 101, 300
TauI GCSGC 3 cut(s) 37, 40, 58
Tru1I TTAA 1 cut(s) 84
Tru9I TTAA 1 cut(s) 84
TscAI CASTG 2 cut(s) 57, 76
TseI GCWGC 3 cut(s) 369, 384, 524
TspGWI ACGGA 1 cut(s) 377
TspRI CASTG 2 cut(s) 57, 76
Van91I CCANNNNNTGG 1 cut(s) 453
VpaK11BI GGWCC 1 cut(s) 126
XceI RCATGY 2 cut(s) 396, 445
XspI CTAG 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.