RchiOBHm_Chr4g0435581

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0016636
Physical Location & Seq
Reverse (-)
59066128 .. 59066958
831 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40400

Sequence Viewer

Length: 612 bp
ATGTACTCTAGCATGGCCGAATTGCAGAACGAGCCACTCCCTTCGGCCCCATCGGCGCCACCCCCAAGTCCTCGGATTACACCACCATCATCAGCGCCACCTCTTCCTTTTGATTTGAATGATCAGCCCCTCACAAACACCGGCGTTCCGGTCATGGCGGTGACCCTTCCTCATTCCCCAGCCCCTTGGTCCACTGACCTATGCGATTGTTGTGACGATCTCAGCAGTTGTTGCTTAACGTGTTGGTGTCCATGTATAACATTTGGACGCATTGCAGAAATTGTGGACAGGGGATCATCTTCATGTGGAGTGAGTGGAATACTTTACAGTTTGATGCTGTGCTTGATGGGTTGCTCGTGTTTGTATTCGTGCTTCTATCGATCTAGACTGAGAGGGCAGTACTTCTTGGAGGAGAAGCCATGCGCAGATTGCTGCGTCCATTTTTGCTGCGAGGTATGCGCTCTGTGCCAAGAGTATCGACACCTTCAGAACCAGGGTTTCGACATGTCCATAGGTTGGTATGGCAACGTCCACAAGCAGAGACGACTAGCCGAAATGCTGCCACCGCCGGCAATAATGCCTCCACAACTTCAGACCGGCATGAACCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

203

Amino Acids

22.37

Weight (kDa)

5.25

Isoelectric Point (pI)

76.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 63 - 161 1.9e-33 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016636)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68610
fragaria_vesca FvH4_4g27720
malus_domestica MD16G1045800.v1.1
prunus_persica Prupe.1G307600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0435581
rosa_laevigata RLG00000006580
rosa_multiflora Rmu_sc0006697.1_g000003
rosa_roxburghii Rroxscaffold_5G00376810
rosa_rugosa Rorug04G0286800.1
rosa_samantha Rh4AG342200 Rh4BG350400 Rh4CG365100 Rh4DG344700
rosa_wichuraiana Rw4G029850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 424
AccB1I GGYRCC 1 cut(s) 55
AccB7I CCANNNNNTGG 1 cut(s) 516
AciI CCGC 2 cut(s) 158, 566
AclWI GGATC 1 cut(s) 301
AcoI YGGCCR 1 cut(s) 15
AcuI CTGAAG 2 cut(s) 470, 575
AcyI GRCGYC 1 cut(s) 56
AfaI GTAC 2 cut(s) 5, 401
AfiI CCNNNNNNNGG 1 cut(s) 516
AflIII ACRYGT 2 cut(s) 239, 504
AgsI TTSAA 1 cut(s) 118
AjnI CCWGG 1 cut(s) 492
Alw26I GTCTC 1 cut(s) 535
AlwI GGATC 1 cut(s) 301
AoxI GGCC 2 cut(s) 15, 45
ApeKI GCWGC 3 cut(s) 432, 447, 559
AspLEI GCGC 4 cut(s) 58, 97, 425, 461
AspS9I GGNCC 2 cut(s) 46, 189
AsuHPI GGTGA 1 cut(s) 172
AvaII GGWCC 1 cut(s) 189
BanI GGYRCC 1 cut(s) 55
BarI GAAGNNNNNNTAC 2 cut(s) 356, 388
BauI CACGAG 1 cut(s) 355
BbvI GCAGC 3 cut(s) 419, 434, 546
BccI CCATC 3 cut(s) 58, 94, 340
BciT130I CCWGG 1 cut(s) 494
BclI TGATCA 1 cut(s) 121
BcoDI GTCTC 1 cut(s) 535
BfaI CTAG 3 cut(s) 9, 384, 548
BfoI RGCGCY 2 cut(s) 59, 98
BglI GCCNNNNNGGC 1 cut(s) 53
BisI GCNGC 3 cut(s) 433, 448, 560
BlsI GCNGC 3 cut(s) 434, 449, 561
BmcAI AGTACT 1 cut(s) 401
Bme1390I CCNGG 1 cut(s) 494
Bme18I GGWCC 1 cut(s) 189
BmgT120I GGNCC 2 cut(s) 46, 189
BmiI GGNNCC 2 cut(s) 48, 57
BmrFI CCNGG 1 cut(s) 494
BmsI GCATC 1 cut(s) 324
Bsa29I ATCGAT 1 cut(s) 379
BsaHI GRCGYC 1 cut(s) 56
BsaJI CCNNGG 3 cut(s) 71, 185, 493
BsaWI WCCGGW 1 cut(s) 148
Bsc4I CCNNNNNNNGG 1 cut(s) 516
Bse118I RCCGGY 3 cut(s) 140, 568, 596
Bse3DI GCAATG 1 cut(s) 270
BseBI CCWGG 1 cut(s) 494
BseCI ATCGAT 1 cut(s) 379
BseDI CCNNGG 3 cut(s) 71, 185, 493
BseLI CCNNNNNNNGG 1 cut(s) 516
BseMI GCAATG 1 cut(s) 270
BseMII CTCAG 2 cut(s) 235, 380
BseRI GAGGAG 1 cut(s) 425
BseXI GCAGC 3 cut(s) 419, 434, 546
BseYI CCCAGC 1 cut(s) 178
BshFI GGCC 2 cut(s) 17, 47
BshNI GGYRCC 1 cut(s) 55
BshVI ATCGAT 1 cut(s) 379
BsiSI CCGG 4 cut(s) 141, 149, 569, 597
BslI CCNNNNNNNGG 1 cut(s) 516
BsmAI GTCTC 1 cut(s) 535
BsmBI CGTCTC 1 cut(s) 535
BsnI GGCC 2 cut(s) 17, 47
Bsp143I GATC 4 cut(s) 121, 217, 293, 380
BspACI CCGC 2 cut(s) 158, 566
BspANI GGCC 2 cut(s) 17, 47
BspCNI CTCAG 2 cut(s) 234, 381
BspDI ATCGAT 1 cut(s) 379
BspLI GGNNCC 2 cut(s) 48, 57
BspPI GGATC 1 cut(s) 301
BspT107I GGYRCC 1 cut(s) 55
BsrDI GCAATG 1 cut(s) 270
BsrFI RCCGGY 3 cut(s) 140, 568, 596
BssAI RCCGGY 3 cut(s) 140, 568, 596
BssECI CCNNGG 3 cut(s) 71, 185, 493
BssMI GATC 4 cut(s) 121, 217, 293, 380
BssNI GRCGYC 1 cut(s) 56
BssSI CACGAG 1 cut(s) 355
BssT1I CCWWGG 1 cut(s) 185
Bst2BI CACGAG 1 cut(s) 355
Bst2UI CCWGG 1 cut(s) 494
Bst4CI ACNGT 2 cut(s) 329, 608
Bst6I CTCTTC 1 cut(s) 108
BstACI GRCGYC 1 cut(s) 56
BstAPI GCANNNNNTGC 1 cut(s) 231
BstC8I GCNNGC 1 cut(s) 570
BstDEI CTNAG 2 cut(s) 221, 389
BstEII GGTNACC 1 cut(s) 160
BstH2I RGCGCY 2 cut(s) 59, 98
BstHHI GCGC 4 cut(s) 58, 97, 425, 461
BstKTI GATC 4 cut(s) 124, 220, 296, 383
BstMAI GTCTC 1 cut(s) 535
BstMBI GATC 4 cut(s) 121, 217, 293, 380
BstMWI GCNNNNNNNGC 7 cut(s) 31, 53, 231, 429, 456, 465, 565
BstNI CCWGG 1 cut(s) 494
BstNSI RCATGY 1 cut(s) 508
BstPI GGTNACC 1 cut(s) 160
BstSCI CCNGG 1 cut(s) 492
BstV1I GCAGC 3 cut(s) 419, 434, 546
BstXI CCANNNNNNTGG 1 cut(s) 186
Bsu15I ATCGAT 1 cut(s) 379
BsuRI GGCC 2 cut(s) 17, 47
BsuTUI ATCGAT 1 cut(s) 379
BtsIMutI CAGTG 1 cut(s) 192
Cac8I GCNNGC 1 cut(s) 570
CfoI GCGC 4 cut(s) 58, 97, 425, 461
Cfr10I RCCGGY 3 cut(s) 140, 568, 596
Cfr13I GGNCC 2 cut(s) 46, 189
ClaI ATCGAT 1 cut(s) 379
CseI GACGC 2 cut(s) 276, 424
Csp6I GTAC 2 cut(s) 4, 400
CviAII CATG 7 cut(s) 13, 154, 252, 303, 420, 505, 601
CviJI RGCY 7 cut(s) 17, 34, 47, 127, 182, 418, 551
CviKI_1 RGCY 7 cut(s) 17, 34, 47, 127, 182, 418, 551
CviQI GTAC 2 cut(s) 4, 400
DdeI CTNAG 2 cut(s) 221, 389
DinI GGCGCC 1 cut(s) 57
DpnI GATC 4 cut(s) 123, 219, 295, 382
DpnII GATC 4 cut(s) 121, 217, 293, 380
EaeI YGGCCR 1 cut(s) 15
Eam1104I CTCTTC 1 cut(s) 108
EarI CTCTTC 1 cut(s) 108
Eco130I CCWWGG 1 cut(s) 185
Eco47I GGWCC 1 cut(s) 189
Eco57I CTGAAG 2 cut(s) 470, 575
Eco91I GGTNACC 1 cut(s) 160
EcoO65I GGTNACC 1 cut(s) 160
EcoRII CCWGG 1 cut(s) 492
EcoT14I CCWWGG 1 cut(s) 185
EgeI GGCGCC 1 cut(s) 57
EheI GGCGCC 1 cut(s) 57
ErhI CCWWGG 1 cut(s) 185
Esp3I CGTCTC 1 cut(s) 535
FaeI CATG 7 cut(s) 16, 157, 255, 306, 423, 508, 604
FatI CATG 7 cut(s) 12, 153, 251, 302, 419, 504, 600
FbaI TGATCA 1 cut(s) 121
Fnu4HI GCNGC 3 cut(s) 433, 448, 560
Fsp4HI GCNGC 3 cut(s) 433, 448, 560
FspBI CTAG 3 cut(s) 9, 384, 548
FspI TGCGCA 1 cut(s) 424
GlaI GCGC 4 cut(s) 57, 96, 424, 460
GluI GCNGC 3 cut(s) 433, 448, 560
GsaI CCCAGC 1 cut(s) 182
HaeII RGCGCY 2 cut(s) 59, 98
HaeIII GGCC 2 cut(s) 17, 47
HapII CCGG 4 cut(s) 141, 149, 569, 597
HgaI GACGC 2 cut(s) 276, 424
HhaI GCGC 4 cut(s) 58, 97, 425, 461
Hin1I GRCGYC 1 cut(s) 56
Hin1II CATG 7 cut(s) 16, 157, 255, 306, 423, 508, 604
Hin6I GCGC 4 cut(s) 56, 95, 423, 459
HinP1I GCGC 4 cut(s) 56, 95, 423, 459
HpaII CCGG 4 cut(s) 141, 149, 569, 597
HphI GGTGA 1 cut(s) 172
Hpy166II GTNNAC 3 cut(s) 192, 286, 532
Hpy188I TCNGA 3 cut(s) 75, 489, 594
Hpy188III TCNNGA 1 cut(s) 384
Hpy8I GTNNAC 3 cut(s) 192, 286, 532
HpyAV CCTTC 3 cut(s) 51, 176, 494
HpyCH4III ACNGT 2 cut(s) 329, 608
HpyCH4IV ACGT 2 cut(s) 239, 528
HpyCH4V TGCA 2 cut(s) 25, 275
HpyF10VI GCNNNNNNNGC 7 cut(s) 31, 53, 231, 429, 456, 465, 565
HpyF3I CTNAG 2 cut(s) 221, 389
HpySE526I ACGT 2 cut(s) 239, 528
Hsp92I GRCGYC 1 cut(s) 56
Hsp92II CATG 7 cut(s) 16, 157, 255, 306, 423, 508, 604
HspAI GCGC 4 cut(s) 56, 95, 423, 459
KasI GGCGCC 1 cut(s) 55
KroI GCCGGC 1 cut(s) 568
KroNI GCCGGC 1 cut(s) 570
Ksp22I TGATCA 1 cut(s) 121
Kzo9I GATC 4 cut(s) 121, 217, 293, 380
LpnPI CCDG 7 cut(s) 154, 162, 192, 274, 479, 506, 582
Lsp1109I GCAGC 3 cut(s) 419, 434, 546
LweI GCATC 1 cut(s) 324
MaeI CTAG 3 cut(s) 9, 384, 548
MaeII ACGT 2 cut(s) 239, 528
MaeIII GTNAC 2 cut(s) 160, 212
MalI GATC 4 cut(s) 123, 219, 295, 382
MboI GATC 4 cut(s) 121, 217, 293, 380
MboII GAAGA 2 cut(s) 95, 291
MluCI AATT 2 cut(s) 20, 279
Mly113I GGCGCC 1 cut(s) 56
MnlI CCTC 8 cut(s) 81, 111, 140, 180, 386, 403, 445, 591
MroNI GCCGGC 1 cut(s) 568
MseI TTAA 1 cut(s) 236
MslI CAYNNNNRTG 2 cut(s) 158, 301
MspI CCGG 4 cut(s) 141, 149, 569, 597
MspR9I CCNGG 1 cut(s) 494
MvaI CCWGG 1 cut(s) 494
MwoI GCNNNNNNNGC 7 cut(s) 31, 53, 231, 429, 456, 465, 565
NaeI GCCGGC 1 cut(s) 570
NarI GGCGCC 1 cut(s) 56
NdeII GATC 4 cut(s) 121, 217, 293, 380
NgoMIV GCCGGC 1 cut(s) 568
NlaIII CATG 7 cut(s) 16, 157, 255, 306, 423, 508, 604
NlaIV GGNNCC 2 cut(s) 48, 57
NmuCI GTSAC 2 cut(s) 160, 212
NsbI TGCGCA 1 cut(s) 424
NspI RCATGY 1 cut(s) 508
PciI ACATGT 1 cut(s) 504
PdiI GCCGGC 1 cut(s) 570
PflMI CCANNNNNTGG 1 cut(s) 516
PkrI GCNGC 3 cut(s) 434, 449, 561
PluTI GGCGCC 1 cut(s) 59
PscI ACATGT 1 cut(s) 504
Psp6I CCWGG 1 cut(s) 492
PspEI GGTNACC 1 cut(s) 160
PspFI CCCAGC 1 cut(s) 178
PspGI CCWGG 1 cut(s) 492
PspN4I GGNNCC 2 cut(s) 48, 57
PspPI GGNCC 2 cut(s) 46, 189
RsaI GTAC 2 cut(s) 5, 401
RsaNI GTAC 2 cut(s) 4, 400
RseI CAYNNNNRTG 2 cut(s) 158, 301
SaqAI TTAA 1 cut(s) 236
SatI GCNGC 3 cut(s) 433, 448, 560
Sau3AI GATC 4 cut(s) 121, 217, 293, 380
Sau96I GGNCC 2 cut(s) 46, 189
ScaI AGTACT 1 cut(s) 401
ScrFI CCNGG 1 cut(s) 494
SetI ASST 7 cut(s) 103, 201, 242, 456, 486, 517, 531
SfaNI GCATC 1 cut(s) 324
SfoI GGCGCC 1 cut(s) 57
SgrAI CRCCGGYG 1 cut(s) 140
SinI GGWCC 1 cut(s) 189
SmiMI CAYNNNNRTG 2 cut(s) 158, 301
Sse9I AATT 2 cut(s) 20, 279
SsiI CCGC 2 cut(s) 158, 566
SspDI GGCGCC 1 cut(s) 55
SspMI CTAG 3 cut(s) 9, 384, 548
StyD4I CCNGG 1 cut(s) 492
StyI CCWWGG 1 cut(s) 185
TaaI ACNGT 2 cut(s) 329, 608
TaiI ACGT 2 cut(s) 242, 531
TaqI TCGA 3 cut(s) 379, 478, 501
TasI AATT 2 cut(s) 20, 279
TatI WGTACW 2 cut(s) 3, 399
Tru1I TTAA 1 cut(s) 236
Tru9I TTAA 1 cut(s) 236
TscAI CASTG 1 cut(s) 199
TseFI GTSAC 2 cut(s) 160, 212
TseI GCWGC 3 cut(s) 432, 447, 559
Tsp45I GTSAC 2 cut(s) 160, 212
TspDTI ATGAA 1 cut(s) 291
TspRI CASTG 1 cut(s) 199
Van91I CCANNNNNTGG 1 cut(s) 516
VpaK11BI GGWCC 1 cut(s) 189
XbaI TCTAGA 1 cut(s) 383
XceI RCATGY 1 cut(s) 508
XspI CTAG 3 cut(s) 9, 384, 548
ZrmI AGTACT 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.