Rroxscaffold_5G00376810

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0016636
Physical Location & Seq
Reverse (-)
57411102 .. 57411911
810 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00376810.1

Sequence Viewer

Length: 528 bp
ATGGCCGAATTGCAGAACGAGCCACTCCCTTCGGCCCCATTGGCGCCATCCCTAAGTCCTCGGATTAGACCACCGTCATCAGCACCACCTCTTCCTTTTGATTTGAATGATCAGCCCCTCACAACCACCGGCGTTCCGGTCATGCCAGTGACCCTTCCTCATTCCCCAGCCCCTTGGTCCACTGACCTGTGCGATTGTTGTAACGATCTCAGCAGTTCATGTGGAGTGAGTGGAATACTTTACAGTTTGATGCTGTGCTTGATGGGTTGCTCGTGTTTGTATTCGTGCTTCTATCGATCTAGACTAAGAGGGCAGTACTTCTTGGAGGAGAAGCCATGCGCAGATTGCTGCGTCCATTTTTGCTGCGAGGTATGCGCTCTGTGCCAAGAGTATCGACACCTTCAGAACCAGGGCTTCGACATGTCCATAGGTTGGTATGGCAACGTCCACAAGCAGAGACGACTAGCCGCAATGCTGCCACCGCCGGCAATAATGCCTCCACAACTTCAGACCGGCATGAACCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

175

Amino Acids

19.33

Weight (kDa)

6.03

Isoelectric Point (pI)

79.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 72 - 133 1.6e-15 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016636)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68610
fragaria_vesca FvH4_4g27720
malus_domestica MD16G1045800.v1.1
prunus_persica Prupe.1G307600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0435581
rosa_laevigata RLG00000006580
rosa_multiflora Rmu_sc0006697.1_g000003
rosa_roxburghii Rroxscaffold_5G00376810
rosa_rugosa Rorug04G0286800.1
rosa_samantha Rh4AG342200 Rh4BG350400 Rh4CG365100 Rh4DG344700
rosa_wichuraiana Rw4G029850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 340
AccB1I GGYRCC 1 cut(s) 43
AccB7I CCANNNNNTGG 1 cut(s) 432
AciI CCGC 2 cut(s) 468, 482
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 2 cut(s) 386, 491
AcyI GRCGYC 1 cut(s) 44
AfaI GTAC 1 cut(s) 317
AfiI CCNNNNNNNGG 1 cut(s) 432
AflIII ACRYGT 1 cut(s) 420
AgsI TTSAA 1 cut(s) 106
AjnI CCWGG 1 cut(s) 408
Alw26I GTCTC 1 cut(s) 451
AoxI GGCC 2 cut(s) 3, 33
ApeKI GCWGC 3 cut(s) 348, 363, 475
AspLEI GCGC 3 cut(s) 46, 341, 377
AspS9I GGNCC 2 cut(s) 34, 177
AvaII GGWCC 1 cut(s) 177
BanI GGYRCC 1 cut(s) 43
BarI GAAGNNNNNNTAC 2 cut(s) 272, 304
BauI CACGAG 1 cut(s) 271
BbvI GCAGC 3 cut(s) 335, 350, 462
BccI CCATC 2 cut(s) 55, 256
BciT130I CCWGG 1 cut(s) 410
BclI TGATCA 1 cut(s) 109
BcoDI GTCTC 1 cut(s) 451
BfaI CTAG 2 cut(s) 300, 464
BfoI RGCGCY 1 cut(s) 47
BglI GCCNNNNNGGC 1 cut(s) 41
BisI GCNGC 4 cut(s) 349, 364, 468, 476
BlsI GCNGC 4 cut(s) 350, 365, 469, 477
BmcAI AGTACT 1 cut(s) 317
Bme1390I CCNGG 1 cut(s) 410
Bme18I GGWCC 1 cut(s) 177
BmgT120I GGNCC 2 cut(s) 34, 177
BmiI GGNNCC 2 cut(s) 36, 45
BmrFI CCNGG 1 cut(s) 410
BmsI GCATC 1 cut(s) 240
BoxI GACNNNNGTC 1 cut(s) 73
Bsa29I ATCGAT 1 cut(s) 295
BsaHI GRCGYC 1 cut(s) 44
BsaJI CCNNGG 3 cut(s) 59, 173, 409
BsaWI WCCGGW 1 cut(s) 136
Bsc4I CCNNNNNNNGG 1 cut(s) 432
Bse118I RCCGGY 3 cut(s) 128, 484, 512
Bse1I ACTGG 1 cut(s) 146
Bse3DI GCAATG 1 cut(s) 477
BseBI CCWGG 1 cut(s) 410
BseCI ATCGAT 1 cut(s) 295
BseDI CCNNGG 3 cut(s) 59, 173, 409
BseGI GGATG 1 cut(s) 47
BseLI CCNNNNNNNGG 1 cut(s) 432
BseMI GCAATG 1 cut(s) 477
BseMII CTCAG 1 cut(s) 223
BseNI ACTGG 1 cut(s) 146
BseRI GAGGAG 1 cut(s) 341
BseXI GCAGC 3 cut(s) 335, 350, 462
BseYI CCCAGC 1 cut(s) 166
BshFI GGCC 2 cut(s) 5, 35
BshNI GGYRCC 1 cut(s) 43
BshVI ATCGAT 1 cut(s) 295
BsiSI CCGG 4 cut(s) 129, 137, 485, 513
BslI CCNNNNNNNGG 1 cut(s) 432
BsmAI GTCTC 1 cut(s) 451
BsmBI CGTCTC 1 cut(s) 451
BsnI GGCC 2 cut(s) 5, 35
Bsp143I GATC 3 cut(s) 109, 205, 296
BspACI CCGC 2 cut(s) 468, 482
BspANI GGCC 2 cut(s) 5, 35
BspCNI CTCAG 1 cut(s) 222
BspDI ATCGAT 1 cut(s) 295
BspLI GGNNCC 2 cut(s) 36, 45
BspT107I GGYRCC 1 cut(s) 43
BsrDI GCAATG 1 cut(s) 477
BsrFI RCCGGY 3 cut(s) 128, 484, 512
BsrI ACTGG 1 cut(s) 146
BssAI RCCGGY 3 cut(s) 128, 484, 512
BssECI CCNNGG 3 cut(s) 59, 173, 409
BssMI GATC 3 cut(s) 109, 205, 296
BssNI GRCGYC 1 cut(s) 44
BssSI CACGAG 1 cut(s) 271
BssT1I CCWWGG 1 cut(s) 173
Bst2BI CACGAG 1 cut(s) 271
Bst2UI CCWGG 1 cut(s) 410
Bst4CI ACNGT 3 cut(s) 75, 245, 524
Bst6I CTCTTC 1 cut(s) 96
BstACI GRCGYC 1 cut(s) 44
BstC8I GCNNGC 1 cut(s) 486
BstDEI CTNAG 3 cut(s) 53, 209, 305
BstF5I GGATG 1 cut(s) 47
BstH2I RGCGCY 1 cut(s) 47
BstHHI GCGC 3 cut(s) 46, 341, 377
BstKTI GATC 3 cut(s) 112, 208, 299
BstMAI GTCTC 1 cut(s) 451
BstMBI GATC 3 cut(s) 109, 205, 296
BstMWI GCNNNNNNNGC 6 cut(s) 19, 41, 345, 372, 381, 481
BstNI CCWGG 1 cut(s) 410
BstNSI RCATGY 1 cut(s) 424
BstPAI GACNNNNGTC 1 cut(s) 73
BstSCI CCNGG 1 cut(s) 408
BstV1I GCAGC 3 cut(s) 335, 350, 462
BstXI CCANNNNNNTGG 1 cut(s) 174
Bsu15I ATCGAT 1 cut(s) 295
BsuRI GGCC 2 cut(s) 5, 35
BsuTUI ATCGAT 1 cut(s) 295
BtsCI GGATG 1 cut(s) 47
BtsIMutI CAGTG 2 cut(s) 153, 180
Cac8I GCNNGC 1 cut(s) 486
CfoI GCGC 3 cut(s) 46, 341, 377
Cfr10I RCCGGY 3 cut(s) 128, 484, 512
Cfr13I GGNCC 2 cut(s) 34, 177
ClaI ATCGAT 1 cut(s) 295
CseI GACGC 1 cut(s) 340
Csp6I GTAC 1 cut(s) 316
CviAII CATG 5 cut(s) 142, 219, 336, 421, 517
CviJI RGCY 8 cut(s) 5, 22, 35, 115, 170, 334, 414, 467
CviKI_1 RGCY 8 cut(s) 5, 22, 35, 115, 170, 334, 414, 467
CviQI GTAC 1 cut(s) 316
DdeI CTNAG 3 cut(s) 53, 209, 305
DinI GGCGCC 1 cut(s) 45
DpnI GATC 3 cut(s) 111, 207, 298
DpnII GATC 3 cut(s) 109, 205, 296
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 96
EarI CTCTTC 1 cut(s) 96
Eco130I CCWWGG 1 cut(s) 173
Eco47I GGWCC 1 cut(s) 177
Eco57I CTGAAG 2 cut(s) 386, 491
EcoRII CCWGG 1 cut(s) 408
EcoT14I CCWWGG 1 cut(s) 173
EgeI GGCGCC 1 cut(s) 45
EheI GGCGCC 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 173
Esp3I CGTCTC 1 cut(s) 451
FaeI CATG 5 cut(s) 145, 222, 339, 424, 520
FaiI YATR 8 cut(s) 143, 220, 337, 373, 422, 428, 438, 518
FatI CATG 5 cut(s) 141, 218, 335, 420, 516
FbaI TGATCA 1 cut(s) 109
Fnu4HI GCNGC 4 cut(s) 349, 364, 468, 476
FokI GGATG 1 cut(s) 34
Fsp4HI GCNGC 4 cut(s) 349, 364, 468, 476
FspBI CTAG 2 cut(s) 300, 464
FspI TGCGCA 1 cut(s) 340
GlaI GCGC 3 cut(s) 45, 340, 376
GluI GCNGC 4 cut(s) 349, 364, 468, 476
GsaI CCCAGC 1 cut(s) 170
HaeII RGCGCY 1 cut(s) 47
HaeIII GGCC 2 cut(s) 5, 35
HapII CCGG 4 cut(s) 129, 137, 485, 513
HgaI GACGC 1 cut(s) 340
HhaI GCGC 3 cut(s) 46, 341, 377
Hin1I GRCGYC 1 cut(s) 44
Hin1II CATG 5 cut(s) 145, 222, 339, 424, 520
Hin6I GCGC 3 cut(s) 44, 339, 375
HinP1I GCGC 3 cut(s) 44, 339, 375
HpaII CCGG 4 cut(s) 129, 137, 485, 513
Hpy166II GTNNAC 2 cut(s) 180, 448
Hpy188I TCNGA 3 cut(s) 63, 405, 510
Hpy188III TCNNGA 1 cut(s) 300
Hpy8I GTNNAC 2 cut(s) 180, 448
HpyAV CCTTC 3 cut(s) 39, 164, 410
HpyCH4III ACNGT 3 cut(s) 75, 245, 524
HpyCH4IV ACGT 1 cut(s) 444
HpyCH4V TGCA 1 cut(s) 13
HpyF10VI GCNNNNNNNGC 6 cut(s) 19, 41, 345, 372, 381, 481
HpyF3I CTNAG 3 cut(s) 53, 209, 305
HpySE526I ACGT 1 cut(s) 444
Hsp92I GRCGYC 1 cut(s) 44
Hsp92II CATG 5 cut(s) 145, 222, 339, 424, 520
HspAI GCGC 3 cut(s) 44, 339, 375
KasI GGCGCC 1 cut(s) 43
KroI GCCGGC 1 cut(s) 484
KroNI GCCGGC 1 cut(s) 486
Ksp22I TGATCA 1 cut(s) 109
Kzo9I GATC 3 cut(s) 109, 205, 296
LpnPI CCDG 8 cut(s) 142, 150, 159, 180, 200, 395, 422, 498
Lsp1109I GCAGC 3 cut(s) 335, 350, 462
LweI GCATC 1 cut(s) 240
MaeI CTAG 2 cut(s) 300, 464
MaeII ACGT 1 cut(s) 444
MaeIII GTNAC 2 cut(s) 148, 200
MalI GATC 3 cut(s) 111, 207, 298
MboI GATC 3 cut(s) 109, 205, 296
MboII GAAGA 1 cut(s) 83
MluCI AATT 1 cut(s) 8
Mly113I GGCGCC 1 cut(s) 44
MnlI CCTC 8 cut(s) 69, 99, 128, 168, 302, 319, 361, 507
MroNI GCCGGC 1 cut(s) 484
MslI CAYNNNNRTG 1 cut(s) 146
MspI CCGG 4 cut(s) 129, 137, 485, 513
MspR9I CCNGG 1 cut(s) 410
MvaI CCWGG 1 cut(s) 410
MwoI GCNNNNNNNGC 6 cut(s) 19, 41, 345, 372, 381, 481
NaeI GCCGGC 1 cut(s) 486
NarI GGCGCC 1 cut(s) 44
NdeII GATC 3 cut(s) 109, 205, 296
NgoMIV GCCGGC 1 cut(s) 484
NlaIII CATG 5 cut(s) 145, 222, 339, 424, 520
NlaIV GGNNCC 2 cut(s) 36, 45
NmuCI GTSAC 1 cut(s) 148
NsbI TGCGCA 1 cut(s) 340
NspI RCATGY 1 cut(s) 424
PciI ACATGT 1 cut(s) 420
PdiI GCCGGC 1 cut(s) 486
PflMI CCANNNNNTGG 1 cut(s) 432
PkrI GCNGC 4 cut(s) 350, 365, 469, 477
PluTI GGCGCC 1 cut(s) 47
PscI ACATGT 1 cut(s) 420
PshAI GACNNNNGTC 1 cut(s) 73
Psp6I CCWGG 1 cut(s) 408
PspFI CCCAGC 1 cut(s) 166
PspGI CCWGG 1 cut(s) 408
PspN4I GGNNCC 2 cut(s) 36, 45
PspPI GGNCC 2 cut(s) 34, 177
RsaI GTAC 1 cut(s) 317
RsaNI GTAC 1 cut(s) 316
RseI CAYNNNNRTG 1 cut(s) 146
SatI GCNGC 4 cut(s) 349, 364, 468, 476
Sau3AI GATC 3 cut(s) 109, 205, 296
Sau96I GGNCC 2 cut(s) 34, 177
ScaI AGTACT 1 cut(s) 317
ScrFI CCNGG 1 cut(s) 410
SetI ASST 6 cut(s) 91, 189, 372, 402, 433, 447
SfaNI GCATC 1 cut(s) 240
SfoI GGCGCC 1 cut(s) 45
SgrAI CRCCGGYG 1 cut(s) 128
SinI GGWCC 1 cut(s) 177
SmiMI CAYNNNNRTG 1 cut(s) 146
Sse9I AATT 1 cut(s) 8
SsiI CCGC 2 cut(s) 468, 482
SspDI GGCGCC 1 cut(s) 43
SspMI CTAG 2 cut(s) 300, 464
StyD4I CCNGG 1 cut(s) 408
StyI CCWWGG 1 cut(s) 173
TaaI ACNGT 3 cut(s) 75, 245, 524
TaiI ACGT 1 cut(s) 447
TaqI TCGA 3 cut(s) 295, 394, 417
TasI AATT 1 cut(s) 8
TatI WGTACW 1 cut(s) 315
TauI GCSGC 1 cut(s) 470
TscAI CASTG 2 cut(s) 153, 187
TseFI GTSAC 1 cut(s) 148
TseI GCWGC 3 cut(s) 348, 363, 475
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 1 cut(s) 207
TspRI CASTG 2 cut(s) 153, 187
Van91I CCANNNNNTGG 1 cut(s) 432
VpaK11BI GGWCC 1 cut(s) 177
XbaI TCTAGA 1 cut(s) 299
XceI RCATGY 1 cut(s) 424
XspI CTAG 2 cut(s) 300, 464
ZrmI AGTACT 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.