Rw4G029850

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0016636
Physical Location & Seq
Reverse (-)
56540380 .. 56541199
820 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G029850.1

Sequence Viewer

Length: 600 bp
ATGGCCGAATTGCAGAACGAGCCACTCCCTTCGGCCCCATCGGCGCCACCCCCAAGTCCTCGGATTACACCACCACCATCAGCACCACCTCTTCCTTTTGATTTGAATGATCAGCCCCTCACTAACACCGGCGTTCCGGTCATGCCGGTGACCCTTCCTCATTCCCCAGCCCCTTGGTCCACTGACCTATGCGATTGTTGTGGCGATCTCAGCAGTTGTTGCTTAACGTGTTGGTGTCCATGTATTACATTTGGACGCATTGCAGAAATTGTGGACAGGGGATCATCTTCATGTGGAGTGAGTGGAATACTTTACAGTTTGATGCTGTGCTTGATGGGTTGCTCGTGTTTGTATTCGTGCTTCTATCGATCTAGACTGAGAGGGCAGTACTTCTTGGAGGAGAAGCCATGCGCAGATTGCTGCGTCCATTTTTGCTGCGAGGTATGCGCTCTGTGCCAAGAGTATCGACACCTTCAGAACCAGGGTTTCGACATGTCCATAGGTTGGTATGGCAACGTCCACAAGCAGAGACGACTAGCCGCAATGCTGCCACCGCCGGCAATAATGCCTCCACAACTTCAGACTGGCATGAACCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

199

Amino Acids

21.82

Weight (kDa)

5.74

Isoelectric Point (pI)

77.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 59 - 157 4.9e-33 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016636)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68610
fragaria_vesca FvH4_4g27720
malus_domestica MD16G1045800.v1.1
prunus_persica Prupe.1G307600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0435581
rosa_laevigata RLG00000006580
rosa_multiflora Rmu_sc0006697.1_g000003
rosa_roxburghii Rroxscaffold_5G00376810
rosa_rugosa Rorug04G0286800.1
rosa_samantha Rh4AG342200 Rh4BG350400 Rh4CG365100 Rh4DG344700
rosa_wichuraiana Rw4G029850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 412
AccB1I GGYRCC 1 cut(s) 43
AccB7I CCANNNNNTGG 1 cut(s) 504
AciI CCGC 2 cut(s) 540, 554
AclWI GGATC 1 cut(s) 289
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 2 cut(s) 458, 563
AcyI GRCGYC 1 cut(s) 44
AfaI GTAC 1 cut(s) 389
AfiI CCNNNNNNNGG 1 cut(s) 504
AflIII ACRYGT 2 cut(s) 227, 492
AgsI TTSAA 1 cut(s) 106
AjnI CCWGG 1 cut(s) 480
Alw26I GTCTC 1 cut(s) 523
AlwI GGATC 1 cut(s) 289
AoxI GGCC 2 cut(s) 3, 33
ApeKI GCWGC 3 cut(s) 420, 435, 547
AspLEI GCGC 3 cut(s) 46, 413, 449
AspS9I GGNCC 2 cut(s) 34, 177
AsuHPI GGTGA 1 cut(s) 160
AvaII GGWCC 1 cut(s) 177
BanI GGYRCC 1 cut(s) 43
BarI GAAGNNNNNNTAC 2 cut(s) 344, 376
BauI CACGAG 1 cut(s) 343
BbvI GCAGC 3 cut(s) 407, 422, 534
BccI CCATC 3 cut(s) 46, 85, 328
BciT130I CCWGG 1 cut(s) 482
BclI TGATCA 1 cut(s) 109
BcoDI GTCTC 1 cut(s) 523
BfaI CTAG 2 cut(s) 372, 536
BfoI RGCGCY 1 cut(s) 47
BglI GCCNNNNNGGC 1 cut(s) 41
BisI GCNGC 4 cut(s) 421, 436, 540, 548
BlsI GCNGC 4 cut(s) 422, 437, 541, 549
BmcAI AGTACT 1 cut(s) 389
Bme1390I CCNGG 1 cut(s) 482
Bme18I GGWCC 1 cut(s) 177
BmgT120I GGNCC 2 cut(s) 34, 177
BmiI GGNNCC 2 cut(s) 36, 45
BmrFI CCNGG 1 cut(s) 482
BmsI GCATC 1 cut(s) 312
Bsa29I ATCGAT 1 cut(s) 367
BsaHI GRCGYC 1 cut(s) 44
BsaJI CCNNGG 3 cut(s) 59, 173, 481
BsaWI WCCGGW 1 cut(s) 136
Bsc4I CCNNNNNNNGG 1 cut(s) 504
Bse118I RCCGGY 3 cut(s) 128, 145, 556
Bse1I ACTGG 1 cut(s) 589
Bse3DI GCAATG 2 cut(s) 258, 549
BseBI CCWGG 1 cut(s) 482
BseCI ATCGAT 1 cut(s) 367
BseDI CCNNGG 3 cut(s) 59, 173, 481
BseLI CCNNNNNNNGG 1 cut(s) 504
BseMI GCAATG 2 cut(s) 258, 549
BseMII CTCAG 2 cut(s) 223, 368
BseNI ACTGG 1 cut(s) 589
BseRI GAGGAG 1 cut(s) 413
BseXI GCAGC 3 cut(s) 407, 422, 534
BseYI CCCAGC 1 cut(s) 166
BshFI GGCC 2 cut(s) 5, 35
BshNI GGYRCC 1 cut(s) 43
BshVI ATCGAT 1 cut(s) 367
BsiSI CCGG 4 cut(s) 129, 137, 146, 557
BslI CCNNNNNNNGG 1 cut(s) 504
BsmAI GTCTC 1 cut(s) 523
BsmBI CGTCTC 1 cut(s) 523
BsnI GGCC 2 cut(s) 5, 35
Bsp143I GATC 4 cut(s) 109, 205, 281, 368
BspACI CCGC 2 cut(s) 540, 554
BspANI GGCC 2 cut(s) 5, 35
BspCNI CTCAG 2 cut(s) 222, 369
BspDI ATCGAT 1 cut(s) 367
BspLI GGNNCC 2 cut(s) 36, 45
BspPI GGATC 1 cut(s) 289
BspT107I GGYRCC 1 cut(s) 43
BsrDI GCAATG 2 cut(s) 258, 549
BsrFI RCCGGY 3 cut(s) 128, 145, 556
BsrI ACTGG 1 cut(s) 589
BssAI RCCGGY 3 cut(s) 128, 145, 556
BssECI CCNNGG 3 cut(s) 59, 173, 481
BssMI GATC 4 cut(s) 109, 205, 281, 368
BssNI GRCGYC 1 cut(s) 44
BssSI CACGAG 1 cut(s) 343
BssT1I CCWWGG 1 cut(s) 173
Bst2BI CACGAG 1 cut(s) 343
Bst2UI CCWGG 1 cut(s) 482
Bst4CI ACNGT 2 cut(s) 317, 596
Bst6I CTCTTC 1 cut(s) 96
BstACI GRCGYC 1 cut(s) 44
BstAPI GCANNNNNTGC 1 cut(s) 219
BstC8I GCNNGC 1 cut(s) 558
BstDEI CTNAG 2 cut(s) 209, 377
BstEII GGTNACC 1 cut(s) 148
BstH2I RGCGCY 1 cut(s) 47
BstHHI GCGC 3 cut(s) 46, 413, 449
BstKTI GATC 4 cut(s) 112, 208, 284, 371
BstMAI GTCTC 1 cut(s) 523
BstMBI GATC 4 cut(s) 109, 205, 281, 368
BstMWI GCNNNNNNNGC 8 cut(s) 19, 41, 210, 219, 417, 444, 453, 553
BstNI CCWGG 1 cut(s) 482
BstNSI RCATGY 1 cut(s) 496
BstPI GGTNACC 1 cut(s) 148
BstSCI CCNGG 1 cut(s) 480
BstV1I GCAGC 3 cut(s) 407, 422, 534
BstXI CCANNNNNNTGG 1 cut(s) 174
Bsu15I ATCGAT 1 cut(s) 367
BsuRI GGCC 2 cut(s) 5, 35
BsuTUI ATCGAT 1 cut(s) 367
BtsIMutI CAGTG 1 cut(s) 180
Cac8I GCNNGC 1 cut(s) 558
CfoI GCGC 3 cut(s) 46, 413, 449
Cfr10I RCCGGY 3 cut(s) 128, 145, 556
Cfr13I GGNCC 2 cut(s) 34, 177
ClaI ATCGAT 1 cut(s) 367
CseI GACGC 2 cut(s) 264, 412
Csp6I GTAC 1 cut(s) 388
CviAII CATG 6 cut(s) 142, 240, 291, 408, 493, 589
CviJI RGCY 7 cut(s) 5, 22, 35, 115, 170, 406, 539
CviKI_1 RGCY 7 cut(s) 5, 22, 35, 115, 170, 406, 539
CviQI GTAC 1 cut(s) 388
DdeI CTNAG 2 cut(s) 209, 377
DinI GGCGCC 1 cut(s) 45
DpnI GATC 4 cut(s) 111, 207, 283, 370
DpnII GATC 4 cut(s) 109, 205, 281, 368
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 96
EarI CTCTTC 1 cut(s) 96
Eco130I CCWWGG 1 cut(s) 173
Eco47I GGWCC 1 cut(s) 177
Eco57I CTGAAG 2 cut(s) 458, 563
Eco91I GGTNACC 1 cut(s) 148
EcoO65I GGTNACC 1 cut(s) 148
EcoRII CCWGG 1 cut(s) 480
EcoT14I CCWWGG 1 cut(s) 173
EgeI GGCGCC 1 cut(s) 45
EheI GGCGCC 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 173
Esp3I CGTCTC 1 cut(s) 523
FaeI CATG 6 cut(s) 145, 243, 294, 411, 496, 592
FatI CATG 6 cut(s) 141, 239, 290, 407, 492, 588
FbaI TGATCA 1 cut(s) 109
Fnu4HI GCNGC 4 cut(s) 421, 436, 540, 548
Fsp4HI GCNGC 4 cut(s) 421, 436, 540, 548
FspBI CTAG 2 cut(s) 372, 536
FspI TGCGCA 1 cut(s) 412
GlaI GCGC 3 cut(s) 45, 412, 448
GluI GCNGC 4 cut(s) 421, 436, 540, 548
GsaI CCCAGC 1 cut(s) 170
HaeII RGCGCY 1 cut(s) 47
HaeIII GGCC 2 cut(s) 5, 35
HapII CCGG 4 cut(s) 129, 137, 146, 557
HgaI GACGC 2 cut(s) 264, 412
HhaI GCGC 3 cut(s) 46, 413, 449
Hin1I GRCGYC 1 cut(s) 44
Hin1II CATG 6 cut(s) 145, 243, 294, 411, 496, 592
Hin6I GCGC 3 cut(s) 44, 411, 447
HinP1I GCGC 3 cut(s) 44, 411, 447
HpaII CCGG 4 cut(s) 129, 137, 146, 557
HphI GGTGA 1 cut(s) 160
Hpy166II GTNNAC 3 cut(s) 180, 274, 520
Hpy188I TCNGA 3 cut(s) 63, 477, 582
Hpy188III TCNNGA 1 cut(s) 372
Hpy8I GTNNAC 3 cut(s) 180, 274, 520
HpyAV CCTTC 3 cut(s) 39, 164, 482
HpyCH4III ACNGT 2 cut(s) 317, 596
HpyCH4IV ACGT 2 cut(s) 227, 516
HpyCH4V TGCA 2 cut(s) 13, 263
HpyF10VI GCNNNNNNNGC 8 cut(s) 19, 41, 210, 219, 417, 444, 453, 553
HpyF3I CTNAG 2 cut(s) 209, 377
HpySE526I ACGT 2 cut(s) 227, 516
Hsp92I GRCGYC 1 cut(s) 44
Hsp92II CATG 6 cut(s) 145, 243, 294, 411, 496, 592
HspAI GCGC 3 cut(s) 44, 411, 447
KasI GGCGCC 1 cut(s) 43
KroI GCCGGC 1 cut(s) 556
KroNI GCCGGC 1 cut(s) 558
Ksp22I TGATCA 1 cut(s) 109
Kzo9I GATC 4 cut(s) 109, 205, 281, 368
LpnPI CCDG 9 cut(s) 142, 150, 159, 180, 262, 467, 494, 570, 570
Lsp1109I GCAGC 3 cut(s) 407, 422, 534
LweI GCATC 1 cut(s) 312
MaeI CTAG 2 cut(s) 372, 536
MaeII ACGT 2 cut(s) 227, 516
MaeIII GTNAC 1 cut(s) 148
MalI GATC 4 cut(s) 111, 207, 283, 370
MboI GATC 4 cut(s) 109, 205, 281, 368
MboII GAAGA 2 cut(s) 83, 279
MluCI AATT 2 cut(s) 8, 267
Mly113I GGCGCC 1 cut(s) 44
MnlI CCTC 8 cut(s) 69, 99, 128, 168, 374, 391, 433, 579
MroNI GCCGGC 1 cut(s) 556
MseI TTAA 1 cut(s) 224
MslI CAYNNNNRTG 2 cut(s) 146, 289
MspI CCGG 4 cut(s) 129, 137, 146, 557
MspR9I CCNGG 1 cut(s) 482
MvaI CCWGG 1 cut(s) 482
MwoI GCNNNNNNNGC 8 cut(s) 19, 41, 210, 219, 417, 444, 453, 553
NaeI GCCGGC 1 cut(s) 558
NarI GGCGCC 1 cut(s) 44
NdeII GATC 4 cut(s) 109, 205, 281, 368
NgoMIV GCCGGC 1 cut(s) 556
NlaIII CATG 6 cut(s) 145, 243, 294, 411, 496, 592
NlaIV GGNNCC 2 cut(s) 36, 45
NmuCI GTSAC 1 cut(s) 148
NsbI TGCGCA 1 cut(s) 412
NspI RCATGY 1 cut(s) 496
PciI ACATGT 1 cut(s) 492
PdiI GCCGGC 1 cut(s) 558
PflMI CCANNNNNTGG 1 cut(s) 504
PkrI GCNGC 4 cut(s) 422, 437, 541, 549
PluTI GGCGCC 1 cut(s) 47
PscI ACATGT 1 cut(s) 492
Psp6I CCWGG 1 cut(s) 480
PspEI GGTNACC 1 cut(s) 148
PspFI CCCAGC 1 cut(s) 166
PspGI CCWGG 1 cut(s) 480
PspN4I GGNNCC 2 cut(s) 36, 45
PspPI GGNCC 2 cut(s) 34, 177
RsaI GTAC 1 cut(s) 389
RsaNI GTAC 1 cut(s) 388
RseI CAYNNNNRTG 2 cut(s) 146, 289
SaqAI TTAA 1 cut(s) 224
SatI GCNGC 4 cut(s) 421, 436, 540, 548
Sau3AI GATC 4 cut(s) 109, 205, 281, 368
Sau96I GGNCC 2 cut(s) 34, 177
ScaI AGTACT 1 cut(s) 389
ScrFI CCNGG 1 cut(s) 482
SetI ASST 7 cut(s) 91, 189, 230, 444, 474, 505, 519
SfaNI GCATC 1 cut(s) 312
SfoI GGCGCC 1 cut(s) 45
SgrAI CRCCGGYG 1 cut(s) 128
SinI GGWCC 1 cut(s) 177
SmiMI CAYNNNNRTG 2 cut(s) 146, 289
Sse9I AATT 2 cut(s) 8, 267
SsiI CCGC 2 cut(s) 540, 554
SspDI GGCGCC 1 cut(s) 43
SspMI CTAG 2 cut(s) 372, 536
StyD4I CCNGG 1 cut(s) 480
StyI CCWWGG 1 cut(s) 173
TaaI ACNGT 2 cut(s) 317, 596
TaiI ACGT 2 cut(s) 230, 519
TaqI TCGA 3 cut(s) 367, 466, 489
TasI AATT 2 cut(s) 8, 267
TatI WGTACW 1 cut(s) 387
TauI GCSGC 1 cut(s) 542
Tru1I TTAA 1 cut(s) 224
Tru9I TTAA 1 cut(s) 224
TscAI CASTG 1 cut(s) 187
TseFI GTSAC 1 cut(s) 148
TseI GCWGC 3 cut(s) 420, 435, 547
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 1 cut(s) 279
TspRI CASTG 1 cut(s) 187
Van91I CCANNNNNTGG 1 cut(s) 504
VpaK11BI GGWCC 1 cut(s) 177
XbaI TCTAGA 1 cut(s) 371
XceI RCATGY 1 cut(s) 496
XspI CTAG 2 cut(s) 372, 536
ZrmI AGTACT 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.