Rh4BG350400

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0016636
Physical Location & Seq
Reverse (-)
51186389 .. 51187242
854 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG350400.1

Sequence Viewer

Length: 597 bp
ATGTACTCTAGCATGGCCGAATTGCAGAACGAGCCACTCCCTTCGGCCCCATCGGCGCCACCCCCAAGTCCTCGGATTACACCACCTCTTCCTTTTGATTTGAATGATCAGCCCCTCACAACCACCGGCGTTCCGGTCATGCCAGTGACCCTTCCTCATTCCCCAGCCCCTTGGTCCACTGACCTATGCGATTGTTGTGACGATCTCAGCAGTTGTTGCTTAACGTGTTGGTGTCCATGTATAACATTTGGACGCATTGCAGAAATTGTGGACAGGGGATCATCTTCATGTGGAGTGAGTGGAATACTTTACAGTTTGATGCTGTGCTTGATGGGTTGCTCGTGTTTGTATTCGTGCTTCTATCGATCTAGACTGAGAGGGCAGTACTTCTTGGAGGAGAAGCCATGCGCAGATTGCTGTGTCCATTTTTGCTGCGAGGTATGCGCTCTGTGCCAAGAGTATCGACACCTTCAGAACCAGGGCTTCGACATGTCCATAGGTTGGTATGGCAACGTCCACAAGCAGAGGCGACTAGCCGCAATGCTGCCACCGCCGGCAATAATGCCTCCACAACTTCAGACCGGCATGAACCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

198

Amino Acids

21.89

Weight (kDa)

5.46

Isoelectric Point (pI)

77.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 58 - 156 1.8e-33 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016636)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68610
fragaria_vesca FvH4_4g27720
malus_domestica MD16G1045800.v1.1
prunus_persica Prupe.1G307600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0435581
rosa_laevigata RLG00000006580
rosa_multiflora Rmu_sc0006697.1_g000003
rosa_roxburghii Rroxscaffold_5G00376810
rosa_rugosa Rorug04G0286800.1
rosa_samantha Rh4AG342200 Rh4BG350400 Rh4CG365100 Rh4DG344700
rosa_wichuraiana Rw4G029850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 409
AccB1I GGYRCC 1 cut(s) 55
AccB7I CCANNNNNTGG 1 cut(s) 501
AciI CCGC 2 cut(s) 537, 551
AclWI GGATC 1 cut(s) 286
AcoI YGGCCR 1 cut(s) 15
AcuI CTGAAG 2 cut(s) 455, 560
AcyI GRCGYC 1 cut(s) 56
AfaI GTAC 2 cut(s) 5, 386
AfiI CCNNNNNNNGG 1 cut(s) 501
AflIII ACRYGT 2 cut(s) 224, 489
AgsI TTSAA 1 cut(s) 103
AjnI CCWGG 1 cut(s) 477
AlwI GGATC 1 cut(s) 286
AoxI GGCC 2 cut(s) 15, 45
ApeKI GCWGC 2 cut(s) 432, 544
AspLEI GCGC 3 cut(s) 58, 410, 446
AspS9I GGNCC 2 cut(s) 46, 174
AvaII GGWCC 1 cut(s) 174
BanI GGYRCC 1 cut(s) 55
BarI GAAGNNNNNNTAC 2 cut(s) 341, 373
BauI CACGAG 1 cut(s) 340
BbvI GCAGC 2 cut(s) 419, 531
BccI CCATC 2 cut(s) 58, 325
BciT130I CCWGG 1 cut(s) 479
BclI TGATCA 1 cut(s) 106
BfaI CTAG 3 cut(s) 9, 369, 533
BfoI RGCGCY 1 cut(s) 59
BglI GCCNNNNNGGC 1 cut(s) 53
BisI GCNGC 3 cut(s) 433, 537, 545
BlsI GCNGC 3 cut(s) 434, 538, 546
BmcAI AGTACT 1 cut(s) 386
Bme1390I CCNGG 1 cut(s) 479
Bme18I GGWCC 1 cut(s) 174
BmgT120I GGNCC 2 cut(s) 46, 174
BmiI GGNNCC 2 cut(s) 48, 57
BmrFI CCNGG 1 cut(s) 479
BmsI GCATC 1 cut(s) 309
Bsa29I ATCGAT 1 cut(s) 364
BsaHI GRCGYC 1 cut(s) 56
BsaJI CCNNGG 3 cut(s) 71, 170, 478
BsaWI WCCGGW 1 cut(s) 133
Bsc4I CCNNNNNNNGG 1 cut(s) 501
Bse118I RCCGGY 3 cut(s) 125, 553, 581
Bse1I ACTGG 1 cut(s) 143
Bse3DI GCAATG 2 cut(s) 255, 546
BseBI CCWGG 1 cut(s) 479
BseCI ATCGAT 1 cut(s) 364
BseDI CCNNGG 3 cut(s) 71, 170, 478
BseLI CCNNNNNNNGG 1 cut(s) 501
BseMI GCAATG 2 cut(s) 255, 546
BseMII CTCAG 2 cut(s) 220, 365
BseNI ACTGG 1 cut(s) 143
BseRI GAGGAG 1 cut(s) 410
BseXI GCAGC 2 cut(s) 419, 531
BseYI CCCAGC 1 cut(s) 163
BshFI GGCC 2 cut(s) 17, 47
BshNI GGYRCC 1 cut(s) 55
BshVI ATCGAT 1 cut(s) 364
BsiSI CCGG 4 cut(s) 126, 134, 554, 582
BslI CCNNNNNNNGG 1 cut(s) 501
BsnI GGCC 2 cut(s) 17, 47
Bsp143I GATC 4 cut(s) 106, 202, 278, 365
BspACI CCGC 2 cut(s) 537, 551
BspANI GGCC 2 cut(s) 17, 47
BspCNI CTCAG 2 cut(s) 219, 366
BspDI ATCGAT 1 cut(s) 364
BspLI GGNNCC 2 cut(s) 48, 57
BspPI GGATC 1 cut(s) 286
BspT107I GGYRCC 1 cut(s) 55
BsrDI GCAATG 2 cut(s) 255, 546
BsrFI RCCGGY 3 cut(s) 125, 553, 581
BsrI ACTGG 1 cut(s) 143
BssAI RCCGGY 3 cut(s) 125, 553, 581
BssECI CCNNGG 3 cut(s) 71, 170, 478
BssMI GATC 4 cut(s) 106, 202, 278, 365
BssNI GRCGYC 1 cut(s) 56
BssSI CACGAG 1 cut(s) 340
BssT1I CCWWGG 1 cut(s) 170
Bst2BI CACGAG 1 cut(s) 340
Bst2UI CCWGG 1 cut(s) 479
Bst4CI ACNGT 2 cut(s) 314, 593
Bst6I CTCTTC 1 cut(s) 93
BstACI GRCGYC 1 cut(s) 56
BstAPI GCANNNNNTGC 1 cut(s) 216
BstC8I GCNNGC 1 cut(s) 555
BstDEI CTNAG 2 cut(s) 206, 374
BstH2I RGCGCY 1 cut(s) 59
BstHHI GCGC 3 cut(s) 58, 410, 446
BstKTI GATC 4 cut(s) 109, 205, 281, 368
BstMBI GATC 4 cut(s) 106, 202, 278, 365
BstMWI GCNNNNNNNGC 7 cut(s) 31, 53, 216, 414, 441, 450, 550
BstNI CCWGG 1 cut(s) 479
BstNSI RCATGY 1 cut(s) 493
BstSCI CCNGG 1 cut(s) 477
BstV1I GCAGC 2 cut(s) 419, 531
BstXI CCANNNNNNTGG 1 cut(s) 171
Bsu15I ATCGAT 1 cut(s) 364
BsuRI GGCC 2 cut(s) 17, 47
BsuTUI ATCGAT 1 cut(s) 364
BtsIMutI CAGTG 2 cut(s) 150, 177
Cac8I GCNNGC 1 cut(s) 555
CfoI GCGC 3 cut(s) 58, 410, 446
Cfr10I RCCGGY 3 cut(s) 125, 553, 581
Cfr13I GGNCC 2 cut(s) 46, 174
ClaI ATCGAT 1 cut(s) 364
CseI GACGC 1 cut(s) 261
Csp6I GTAC 2 cut(s) 4, 385
CviAII CATG 7 cut(s) 13, 139, 237, 288, 405, 490, 586
CviJI RGCY 8 cut(s) 17, 34, 47, 112, 167, 403, 483, 536
CviKI_1 RGCY 8 cut(s) 17, 34, 47, 112, 167, 403, 483, 536
CviQI GTAC 2 cut(s) 4, 385
DdeI CTNAG 2 cut(s) 206, 374
DinI GGCGCC 1 cut(s) 57
DpnI GATC 4 cut(s) 108, 204, 280, 367
DpnII GATC 4 cut(s) 106, 202, 278, 365
EaeI YGGCCR 1 cut(s) 15
Eam1104I CTCTTC 1 cut(s) 93
EarI CTCTTC 1 cut(s) 93
Eco130I CCWWGG 1 cut(s) 170
Eco47I GGWCC 1 cut(s) 174
Eco57I CTGAAG 2 cut(s) 455, 560
EcoRII CCWGG 1 cut(s) 477
EcoT14I CCWWGG 1 cut(s) 170
EgeI GGCGCC 1 cut(s) 57
EheI GGCGCC 1 cut(s) 57
ErhI CCWWGG 1 cut(s) 170
FaeI CATG 7 cut(s) 16, 142, 240, 291, 408, 493, 589
FatI CATG 7 cut(s) 12, 138, 236, 287, 404, 489, 585
FbaI TGATCA 1 cut(s) 106
Fnu4HI GCNGC 3 cut(s) 433, 537, 545
Fsp4HI GCNGC 3 cut(s) 433, 537, 545
FspBI CTAG 3 cut(s) 9, 369, 533
FspI TGCGCA 1 cut(s) 409
GlaI GCGC 3 cut(s) 57, 409, 445
GluI GCNGC 3 cut(s) 433, 537, 545
GsaI CCCAGC 1 cut(s) 167
HaeII RGCGCY 1 cut(s) 59
HaeIII GGCC 2 cut(s) 17, 47
HapII CCGG 4 cut(s) 126, 134, 554, 582
HgaI GACGC 1 cut(s) 261
HhaI GCGC 3 cut(s) 58, 410, 446
Hin1I GRCGYC 1 cut(s) 56
Hin1II CATG 7 cut(s) 16, 142, 240, 291, 408, 493, 589
Hin6I GCGC 3 cut(s) 56, 408, 444
HinP1I GCGC 3 cut(s) 56, 408, 444
HpaII CCGG 4 cut(s) 126, 134, 554, 582
Hpy166II GTNNAC 3 cut(s) 177, 271, 517
Hpy188I TCNGA 3 cut(s) 75, 474, 579
Hpy188III TCNNGA 1 cut(s) 369
Hpy8I GTNNAC 3 cut(s) 177, 271, 517
HpyAV CCTTC 3 cut(s) 51, 161, 479
HpyCH4III ACNGT 2 cut(s) 314, 593
HpyCH4IV ACGT 2 cut(s) 224, 513
HpyCH4V TGCA 2 cut(s) 25, 260
HpyF10VI GCNNNNNNNGC 7 cut(s) 31, 53, 216, 414, 441, 450, 550
HpyF3I CTNAG 2 cut(s) 206, 374
HpySE526I ACGT 2 cut(s) 224, 513
Hsp92I GRCGYC 1 cut(s) 56
Hsp92II CATG 7 cut(s) 16, 142, 240, 291, 408, 493, 589
HspAI GCGC 3 cut(s) 56, 408, 444
KasI GGCGCC 1 cut(s) 55
KroI GCCGGC 1 cut(s) 553
KroNI GCCGGC 1 cut(s) 555
Ksp22I TGATCA 1 cut(s) 106
Kzo9I GATC 4 cut(s) 106, 202, 278, 365
LpnPI CCDG 8 cut(s) 139, 147, 156, 177, 259, 464, 491, 567
Lsp1109I GCAGC 2 cut(s) 419, 531
LweI GCATC 1 cut(s) 309
MaeI CTAG 3 cut(s) 9, 369, 533
MaeII ACGT 2 cut(s) 224, 513
MaeIII GTNAC 2 cut(s) 145, 197
MalI GATC 4 cut(s) 108, 204, 280, 367
MboI GATC 4 cut(s) 106, 202, 278, 365
MboII GAAGA 2 cut(s) 80, 276
MluCI AATT 2 cut(s) 20, 264
Mly113I GGCGCC 1 cut(s) 56
MnlI CCTC 9 cut(s) 81, 96, 125, 165, 371, 388, 430, 519, 576
MroNI GCCGGC 1 cut(s) 553
MseI TTAA 1 cut(s) 221
MslI CAYNNNNRTG 2 cut(s) 143, 286
MspI CCGG 4 cut(s) 126, 134, 554, 582
MspR9I CCNGG 1 cut(s) 479
MvaI CCWGG 1 cut(s) 479
MwoI GCNNNNNNNGC 7 cut(s) 31, 53, 216, 414, 441, 450, 550
NaeI GCCGGC 1 cut(s) 555
NarI GGCGCC 1 cut(s) 56
NdeII GATC 4 cut(s) 106, 202, 278, 365
NgoMIV GCCGGC 1 cut(s) 553
NlaIII CATG 7 cut(s) 16, 142, 240, 291, 408, 493, 589
NlaIV GGNNCC 2 cut(s) 48, 57
NmuCI GTSAC 2 cut(s) 145, 197
NsbI TGCGCA 1 cut(s) 409
NspI RCATGY 1 cut(s) 493
PciI ACATGT 1 cut(s) 489
PdiI GCCGGC 1 cut(s) 555
PflMI CCANNNNNTGG 1 cut(s) 501
PkrI GCNGC 3 cut(s) 434, 538, 546
PluTI GGCGCC 1 cut(s) 59
PscI ACATGT 1 cut(s) 489
Psp6I CCWGG 1 cut(s) 477
PspFI CCCAGC 1 cut(s) 163
PspGI CCWGG 1 cut(s) 477
PspN4I GGNNCC 2 cut(s) 48, 57
PspPI GGNCC 2 cut(s) 46, 174
RsaI GTAC 2 cut(s) 5, 386
RsaNI GTAC 2 cut(s) 4, 385
RseI CAYNNNNRTG 2 cut(s) 143, 286
SaqAI TTAA 1 cut(s) 221
SatI GCNGC 3 cut(s) 433, 537, 545
Sau3AI GATC 4 cut(s) 106, 202, 278, 365
Sau96I GGNCC 2 cut(s) 46, 174
ScaI AGTACT 1 cut(s) 386
ScrFI CCNGG 1 cut(s) 479
SetI ASST 7 cut(s) 88, 186, 227, 441, 471, 502, 516
SfaNI GCATC 1 cut(s) 309
SfoI GGCGCC 1 cut(s) 57
SgrAI CRCCGGYG 1 cut(s) 125
SinI GGWCC 1 cut(s) 174
SmiMI CAYNNNNRTG 2 cut(s) 143, 286
Sse9I AATT 2 cut(s) 20, 264
SsiI CCGC 2 cut(s) 537, 551
SspDI GGCGCC 1 cut(s) 55
SspMI CTAG 3 cut(s) 9, 369, 533
StyD4I CCNGG 1 cut(s) 477
StyI CCWWGG 1 cut(s) 170
TaaI ACNGT 2 cut(s) 314, 593
TaiI ACGT 2 cut(s) 227, 516
TaqI TCGA 3 cut(s) 364, 463, 486
TasI AATT 2 cut(s) 20, 264
TatI WGTACW 2 cut(s) 3, 384
TauI GCSGC 1 cut(s) 539
Tru1I TTAA 1 cut(s) 221
Tru9I TTAA 1 cut(s) 221
TscAI CASTG 2 cut(s) 150, 184
TseFI GTSAC 2 cut(s) 145, 197
TseI GCWGC 2 cut(s) 432, 544
Tsp45I GTSAC 2 cut(s) 145, 197
TspDTI ATGAA 1 cut(s) 276
TspRI CASTG 2 cut(s) 150, 184
Van91I CCANNNNNTGG 1 cut(s) 501
VpaK11BI GGWCC 1 cut(s) 174
XbaI TCTAGA 1 cut(s) 368
XceI RCATGY 1 cut(s) 493
XspI CTAG 3 cut(s) 9, 369, 533
ZrmI AGTACT 1 cut(s) 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.