FvH4_4g28200

protein At1g26090, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009127
Physical Location & Seq
Forward (+)
28896148 .. 28899327
3180 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g28200.t5

Sequence Viewer

Length: 1365 bp
ATGGCGCTGCTTACCTTCTGCCCAGTCCTCTCTTCTTCCTCTGTCCTAAGCCGCACAACACCCAACCTAGTCCACGCGACAAGAACACAAACCAGAACACGATCAGGAACCAAAGCTGTGACCTTGGCTGCTTCGTCCGAGGATTCTGACAAGTCAACAAAGCTGGTCACTTTCTTGGGGAAAGGTGGGTCCGGGAAGACCACCTCTGCCGTTTTCGCTGCCCAGCATTATGCAATGGCTGGGTTTAGCACATGTTTGGTGATACATACACAAGATCCCACTGCTGAGTTTCTTTTAAGCTGCAAAATTGGAACCTCTCCTGTTCTGTGCAGTGAAAACCTTTCGGCTCTTAGGTTTGAAACCACCAGAATGCTTCTAGGACCTCTGAAGCAGCTAAAGCAAGCAGATGCTCGACTTAATATGACACAAGGAGTTCTTGAAGGGATTTTTGGAGAAGAGCTTGGCGTGCTTCCTGGGATGGATCCTATTTTTTCCGCATTAGCACTGGAGAGTCTTGTGGGATTCTTTGGGAATGTGGCTCAGAGAAATCACAATAAAGATAAATTTGACATAATTGTGTACGATGGTATGAGCTCTGAGGAAATGCTAAGGATGATAAGTGCAGCTGGTAAAGCAAGGTTGTACTTGAAATATCTACGGAACGTGGCTGACAAAACAGATTTTGGGAGGATGGCTGGTCCTTCACTTCTTAGACTTGTGGATGAAGCCATGAGTCTAACCAACAGTACAAATCTTTTCAATGGGAATAGGAGTGCAGACATATGGAATAGTGTTGAAAAAATGTTGGAGAGAGGGTCGTCTGCTTTCTCAGAACCTCAGAAATTTGGCTGCTTCCTAGTTTTGGACCCAAACAATCCAGCATCTATTAGTTCAGCATTACGATATTGGGGTTGTACAATCCAAGCTGGTGGACAGATTTCTGGAGCATTTGCTACCGCATCACCAAATTTGAATACAGAATCAGAGGAAGAACTCAAGAATAAATTTTCACCCTTGCCTTTTGCTTTCATTCCACATATCTCTCGAAGTTCCCTGCCTCTGGACTGGGTTGCAATCACGCAAAACACTGTCGGTGAAAGCGCACGAAATCTTCTTTCTACAACAGCAAGTGGCAGCAGTAGTCTAATGTCCTCAGTAACGTTTGATGCAGCCAGAAAGTCAGTAATCCTTTTCATGCCAGGTTTTGACAAGTCAGAGATCAAGCTATATCAGTATAGGGGAGGATCCGAGTTATTAGTAGAAGCCGGGGATCAAAGACGTGTAATTTATCTGCCTCCTAAAATTCAAGGGAAGGTTGGTGGTGCCAAGTTCATAGACAGAAGTCTTGTAATCACAATGAGATAG

Protein Analysis

455

Amino Acids

49.13

Weight (kDa)

9.02

Isoelectric Point (pI)

45.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArsA_ATPase PF02374 53 - 222 5e-12 Anion-transporting ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1322
AccII CGCG 1 cut(s) 77
AciI CCGC 3 cut(s) 52, 495, 957
AclI AACGTT 1 cut(s) 1160
AclWI GGATC 6 cut(s) 269, 476, 489, 1239, 1252, 1278
AcsI RAATTY 5 cut(s) 563, 842, 967, 1004, 1302
AcuI CTGAAG 1 cut(s) 407
AfaI GTAC 4 cut(s) 581, 644, 748, 916
AfiI CCNNNNNNNGG 2 cut(s) 862, 1060
AflIII ACRYGT 2 cut(s) 251, 1279
AgsI TTSAA 7 cut(s) 359, 440, 649, 760, 797, 973, 1307
AjiI CACGTC 1 cut(s) 1280
AjnI CCWGG 2 cut(s) 472, 1198
AjuI GAANNNNNNNTTGG 2 cut(s) 432, 464
AluBI AGCT 9 cut(s) 116, 163, 300, 394, 460, 594, 626, 926, 1225
AluI AGCT 9 cut(s) 116, 163, 300, 394, 460, 594, 626, 926, 1225
Alw21I GWGCWC 1 cut(s) 596
AlwI GGATC 6 cut(s) 269, 476, 489, 1239, 1252, 1278
ApeKI GCWGC 9 cut(s) 7, 128, 218, 300, 391, 623, 849, 1134, 1169
ApoI RAATTY 5 cut(s) 563, 842, 967, 1004, 1302
AspLEI GCGC 2 cut(s) 7, 1103
AspS9I GGNCC 4 cut(s) 189, 380, 698, 865
AsuC2I CCSGG 2 cut(s) 193, 1267
AsuHPI GGTGA 4 cut(s) 271, 954, 1002, 1106
AvaII GGWCC 4 cut(s) 189, 380, 698, 865
BamHI GGATCC 2 cut(s) 481, 1244
BanI GGYRCC 1 cut(s) 1322
BanII GRGCYC 1 cut(s) 596
BbsI GAAGAC 1 cut(s) 203
Bbv12I GWGCWC 1 cut(s) 596
BbvI GCAGC 8 cut(s) 115, 205, 287, 403, 635, 836, 1146, 1181
BccI CCATC 3 cut(s) 472, 578, 685
BceAI ACGGC 1 cut(s) 194
BciT130I CCWGG 2 cut(s) 474, 1200
BcnI CCSGG 2 cut(s) 193, 1267
BfaI CTAG 3 cut(s) 68, 377, 857
BfoI RGCGCY 1 cut(s) 8
Bme1390I CCNGG 4 cut(s) 193, 474, 1200, 1267
Bme18I GGWCC 4 cut(s) 189, 380, 698, 865
BmgBI CACGTC 1 cut(s) 1280
BmgT120I GGNCC 4 cut(s) 189, 380, 698, 865
BmiI GGNNCC 7 cut(s) 109, 190, 313, 483, 867, 1246, 1324
BmrFI CCNGG 4 cut(s) 193, 474, 1200, 1267
BmrI ACTGGG 2 cut(s) 17, 1075
BmsI GCATC 4 cut(s) 397, 890, 968, 1156
BmuI ACTGGG 2 cut(s) 17, 1075
BoxI GACNNNNGTC 1 cut(s) 1341
BpiI GAAGAC 1 cut(s) 203
BpmI CTGGAG 2 cut(s) 527, 963
Bpu10I CCTNAGC 2 cut(s) 47, 608
BpuEI CTTGAG 1 cut(s) 980
BpuMI CCSGG 2 cut(s) 193, 1267
BsaJI CCNNGG 4 cut(s) 123, 138, 473, 1266
BsaXI ACNNNNNCTCC 2 cut(s) 800, 830
Bsc4I CCNNNNNNNGG 2 cut(s) 862, 1060
Bse1I ACTGG 3 cut(s) 23, 510, 1070
Bse3DI GCAATG 1 cut(s) 240
BseBI CCWGG 2 cut(s) 474, 1200
BseDI CCNNGG 4 cut(s) 123, 138, 473, 1266
BseGI GGATG 4 cut(s) 483, 618, 696, 727
BseLI CCNNNNNNNGG 2 cut(s) 862, 1060
BseMI GCAATG 1 cut(s) 240
BseMII CTCAG 6 cut(s) 276, 554, 588, 843, 851, 1167
BseNI ACTGG 3 cut(s) 23, 510, 1070
BseXI GCAGC 8 cut(s) 115, 205, 287, 403, 635, 836, 1146, 1181
BseYI CCCAGC 2 cut(s) 222, 239
BsgI GTGCAG 3 cut(s) 349, 642, 795
Bsh1236I CGCG 1 cut(s) 77
BshNI GGYRCC 1 cut(s) 1322
BsiHKAI GWGCWC 1 cut(s) 596
BsiSI CCGG 2 cut(s) 192, 1266
BslI CCNNNNNNNGG 2 cut(s) 862, 1060
BsmI GAATGC 1 cut(s) 375
Bsp1286I GDGCHC 1 cut(s) 596
Bsp1407I TGTACA 1 cut(s) 914
Bsp143I GATC 6 cut(s) 101, 274, 481, 1218, 1244, 1270
BspACI CCGC 3 cut(s) 52, 495, 957
BspCNI CTCAG 6 cut(s) 277, 553, 589, 842, 850, 1166
BspFNI CGCG 1 cut(s) 77
BspLI GGNNCC 7 cut(s) 109, 190, 313, 483, 867, 1246, 1324
BspPI GGATC 6 cut(s) 269, 476, 489, 1239, 1252, 1278
BspQI GCTCTTC 1 cut(s) 450
BspT107I GGYRCC 1 cut(s) 1322
BsrDI GCAATG 1 cut(s) 240
BsrGI TGTACA 1 cut(s) 914
BsrI ACTGG 3 cut(s) 23, 510, 1070
BssECI CCNNGG 4 cut(s) 123, 138, 473, 1266
BssMI GATC 6 cut(s) 101, 274, 481, 1218, 1244, 1270
BssT1I CCWWGG 1 cut(s) 123
Bst2UI CCWGG 2 cut(s) 474, 1200
Bst4CI ACNGT 2 cut(s) 746, 1090
Bst6I CTCTTC 2 cut(s) 37, 450
BstAUI TGTACA 1 cut(s) 914
BstC8I GCNNGC 2 cut(s) 402, 467
BstF5I GGATG 4 cut(s) 483, 618, 696, 727
BstFNI CGCG 1 cut(s) 77
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 1103
BstKTI GATC 6 cut(s) 104, 277, 484, 1221, 1247, 1273
BstMBI GATC 6 cut(s) 101, 274, 481, 1218, 1244, 1270
BstMWI GCNNNNNNNGC 4 cut(s) 215, 397, 466, 632
BstNI CCWGG 2 cut(s) 474, 1200
BstNSI RCATGY 1 cut(s) 255
BstPAI GACNNNNGTC 1 cut(s) 1341
BstSCI CCNGG 4 cut(s) 191, 472, 1198, 1265
BstUI CGCG 1 cut(s) 77
BstV1I GCAGC 8 cut(s) 115, 205, 287, 403, 635, 836, 1146, 1181
BstV2I GAAGAC 1 cut(s) 203
BstX2I RGATCY 3 cut(s) 274, 481, 1244
BstXI CCANNNNNNTGG 1 cut(s) 929
BstYI RGATCY 3 cut(s) 274, 481, 1244
BtrI CACGTC 1 cut(s) 1280
BtsCI GGATG 4 cut(s) 483, 618, 696, 727
BtsI GCAGTG 2 cut(s) 279, 337
BtsIMutI CAGTG 4 cut(s) 279, 337, 503, 1086
Cac8I GCNNGC 2 cut(s) 402, 467
CfoI GCGC 2 cut(s) 7, 1103
Cfr13I GGNCC 4 cut(s) 189, 380, 698, 865
Csp6I GTAC 4 cut(s) 580, 643, 747, 915
CviAII CATG 3 cut(s) 252, 730, 1195
CviQI GTAC 4 cut(s) 580, 643, 747, 915
DpnI GATC 6 cut(s) 103, 276, 483, 1220, 1246, 1272
DpnII GATC 6 cut(s) 101, 274, 481, 1218, 1244, 1270
Eam1104I CTCTTC 2 cut(s) 37, 450
EarI CTCTTC 2 cut(s) 37, 450
Ecl136II GAGCTC 1 cut(s) 594
Eco130I CCWWGG 1 cut(s) 123
Eco24I GRGCYC 1 cut(s) 596
Eco47I GGWCC 4 cut(s) 189, 380, 698, 865
Eco53kI GAGCTC 1 cut(s) 594
Eco57I CTGAAG 1 cut(s) 407
EcoICRI GAGCTC 1 cut(s) 594
EcoO109I RGGNCCY 1 cut(s) 380
EcoRII CCWGG 2 cut(s) 472, 1198
EcoT14I CCWWGG 1 cut(s) 123
EcoT38I GRGCYC 1 cut(s) 596
ErhI CCWWGG 1 cut(s) 123
FaeI CATG 3 cut(s) 255, 733, 1198
FalI AAGNNNNNCTT 2 cut(s) 420, 452
FatI CATG 3 cut(s) 251, 729, 1194
FauNDI CATATG 1 cut(s) 782
FokI GGATG 4 cut(s) 490, 625, 703, 734
FriOI GRGCYC 1 cut(s) 596
FspBI CTAG 3 cut(s) 68, 377, 857
GlaI GCGC 2 cut(s) 6, 1102
GsaI CCCAGC 2 cut(s) 226, 243
GsuI CTGGAG 2 cut(s) 527, 963
HaeII RGCGCY 1 cut(s) 8
HapII CCGG 2 cut(s) 192, 1266
HhaI GCGC 2 cut(s) 7, 1103
Hin1II CATG 3 cut(s) 255, 733, 1198
Hin6I GCGC 2 cut(s) 5, 1101
HinP1I GCGC 2 cut(s) 5, 1101
HincII GTYRAC 1 cut(s) 156
HindII GTYRAC 1 cut(s) 156
HinfI GANTC 5 cut(s) 143, 511, 522, 733, 980
HpaII CCGG 2 cut(s) 192, 1266
HphI GGTGA 4 cut(s) 271, 954, 1002, 1106
Hpy166II GTNNAC 4 cut(s) 73, 156, 580, 932
Hpy188III TCNNGA 6 cut(s) 105, 437, 942, 997, 1044, 1061
Hpy8I GTNNAC 4 cut(s) 73, 156, 580, 932
HpyAV CCTTC 4 cut(s) 25, 434, 711, 1306
HpyCH4III ACNGT 2 cut(s) 746, 1090
HpyCH4IV ACGT 3 cut(s) 663, 1160, 1279
HpyCH4V TGCA 7 cut(s) 233, 303, 330, 623, 776, 1073, 1169
HpyF10VI GCNNNNNNNGC 4 cut(s) 215, 397, 466, 632
HpySE526I ACGT 3 cut(s) 663, 1160, 1279
Hsp92II CATG 3 cut(s) 255, 733, 1198
HspAI GCGC 2 cut(s) 5, 1101
Kzo9I GATC 6 cut(s) 101, 274, 481, 1218, 1244, 1270
LguI GCTCTTC 1 cut(s) 450
LmnI GCTCC 1 cut(s) 944
Lsp1109I GCAGC 8 cut(s) 115, 205, 287, 403, 635, 836, 1146, 1181
LweI GCATC 4 cut(s) 397, 890, 968, 1156
MaeI CTAG 3 cut(s) 68, 377, 857
MaeII ACGT 3 cut(s) 663, 1160, 1279
MaeIII GTNAC 3 cut(s) 118, 166, 1156
MalI GATC 6 cut(s) 103, 276, 483, 1220, 1246, 1272
MboI GATC 6 cut(s) 101, 274, 481, 1218, 1244, 1270
MboII GAAGA 6 cut(s) 24, 27, 208, 467, 1001, 1103
MflI RGATCY 3 cut(s) 274, 481, 1244
MhlI GDGCHC 1 cut(s) 596
MluCI AATT 8 cut(s) 306, 563, 573, 842, 967, 1004, 1284, 1302
MlyI GAGTC 2 cut(s) 520, 742
MmeI TCCRAC 1 cut(s) 786
MseI TTAA 2 cut(s) 296, 417
MslI CAYNNNNRTG 2 cut(s) 368, 575
MspA1I CMGCKG 1 cut(s) 626
MspI CCGG 2 cut(s) 192, 1266
MspR9I CCNGG 4 cut(s) 193, 474, 1200, 1267
Mva1269I GAATGC 1 cut(s) 375
MvaI CCWGG 2 cut(s) 474, 1200
MvnI CGCG 1 cut(s) 77
MwoI GCNNNNNNNGC 4 cut(s) 215, 397, 466, 632
NciI CCSGG 2 cut(s) 193, 1267
NdeI CATATG 1 cut(s) 782
NdeII GATC 6 cut(s) 101, 274, 481, 1218, 1244, 1270
NlaIII CATG 3 cut(s) 255, 733, 1198
NlaIV GGNNCC 7 cut(s) 109, 190, 313, 483, 867, 1246, 1324
NmuCI GTSAC 2 cut(s) 118, 166
NspI RCATGY 1 cut(s) 255
PciI ACATGT 1 cut(s) 251
PciSI GCTCTTC 1 cut(s) 450
PctI GAATGC 1 cut(s) 375
PfeI GAWTC 3 cut(s) 143, 522, 980
PfoI TCCNGGA 1 cut(s) 191
PleI GAGTC 2 cut(s) 519, 741
PpsI GAGTC 2 cut(s) 519, 741
PpuMI RGGWCCY 1 cut(s) 380
PscI ACATGT 1 cut(s) 251
PshAI GACNNNNGTC 1 cut(s) 1341
Psp124BI GAGCTC 1 cut(s) 596
Psp1406I AACGTT 1 cut(s) 1160
Psp5II RGGWCCY 1 cut(s) 380
Psp6I CCWGG 2 cut(s) 472, 1198
PspFI CCCAGC 2 cut(s) 222, 239
PspGI CCWGG 2 cut(s) 472, 1198
PspN4I GGNNCC 7 cut(s) 109, 190, 313, 483, 867, 1246, 1324
PspPI GGNCC 4 cut(s) 189, 380, 698, 865
PspPPI RGGWCCY 1 cut(s) 380
PsuI RGATCY 3 cut(s) 274, 481, 1244
PvuII CAGCTG 1 cut(s) 626
RsaI GTAC 4 cut(s) 581, 644, 748, 916
RsaNI GTAC 4 cut(s) 580, 643, 747, 915
RseI CAYNNNNRTG 2 cut(s) 368, 575
SacI GAGCTC 1 cut(s) 596
SapI GCTCTTC 1 cut(s) 450
SaqAI TTAA 2 cut(s) 296, 417
Sau3AI GATC 6 cut(s) 101, 274, 481, 1218, 1244, 1270
Sau96I GGNCC 4 cut(s) 189, 380, 698, 865
SchI GAGTC 2 cut(s) 520, 742
ScrFI CCNGG 4 cut(s) 193, 474, 1200, 1267
SduI GDGCHC 1 cut(s) 596
SfaNI GCATC 4 cut(s) 397, 890, 968, 1156
SinI GGWCC 4 cut(s) 189, 380, 698, 865
SmiMI CAYNNNNRTG 2 cut(s) 368, 575
SmlI CTYRAG 1 cut(s) 995
SmoI CTYRAG 1 cut(s) 995
Sse9I AATT 8 cut(s) 306, 563, 573, 842, 967, 1004, 1284, 1302
SsiI CCGC 3 cut(s) 52, 495, 957
SspMI CTAG 3 cut(s) 68, 377, 857
SstI GAGCTC 1 cut(s) 596
StyD4I CCNGG 4 cut(s) 191, 472, 1198, 1265
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 2 cut(s) 746, 1090
TaiI ACGT 3 cut(s) 666, 1163, 1282
TaqI TCGA 2 cut(s) 412, 1045
TasI AATT 8 cut(s) 306, 563, 573, 842, 967, 1004, 1284, 1302
TatI WGTACW 3 cut(s) 642, 746, 914
TauI GCSGC 1 cut(s) 54
TfiI GAWTC 3 cut(s) 143, 522, 980
Tru1I TTAA 2 cut(s) 296, 417
Tru9I TTAA 2 cut(s) 296, 417
TscAI CASTG 4 cut(s) 286, 337, 510, 1093
TseFI GTSAC 2 cut(s) 118, 166
TseI GCWGC 9 cut(s) 7, 128, 218, 300, 391, 623, 849, 1134, 1169
Tsp45I GTSAC 2 cut(s) 118, 166
TspDTI ATGAA 4 cut(s) 738, 1018, 1183, 1321
TspGWI ACGGA 1 cut(s) 673
TspRI CASTG 4 cut(s) 286, 337, 510, 1093
VpaK11BI GGWCC 4 cut(s) 189, 380, 698, 865
XapI RAATTY 5 cut(s) 563, 842, 967, 1004, 1302
XceI RCATGY 1 cut(s) 255
XspI CTAG 3 cut(s) 68, 377, 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.