FvH4_4g29180

cytochrome P450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0019229
Physical Location & Seq
Forward (+)
29430104 .. 29430395
292 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g29180.t1

Sequence Viewer

Length: 264 bp
ATGATCCTCAACTCTGGGGAGACGATGTGCATCTTTTCAAGCCAGAGAGGTTCTCAGAAGAAGGGTAACATGGTCGCATACTTTCCCTTCGGAATGGGACCTAGAAATTCTGTGGGATTCAACTTTGCCACCACTGAAGCAAAGATTGTTCTTTCAATGATTCTGCAACGCTACTCCTTCTCCCTTTCCCCGGGTTATGTCCACTCACCTTTCAAGTACATTACAGTCCGTCCGCAGCCTGGAGTAATGCTACACCCTCTCTGA

Protein Analysis

88

Amino Acids

9.69

Weight (kDa)

10.01

Isoelectric Point (pI)

38.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 13 - 68 1.2e-11 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019229)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g29180
malus_domestica MD13G1057300.v1.1
rosa_chinensis RchiOBHm_Chr1g0339421 RchiOBHm_Chr2g0110971 RchiOBHm_Chr4g0437461
rosa_multiflora Rmu_sc0002472.1_g000007 Rmu_sc0002638.1_g000038 Rmu_sc0036394.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 233
AcsI RAATTY 1 cut(s) 106
AcuI CTGAAG 1 cut(s) 156
AfaI GTAC 1 cut(s) 218
AfiI CCNNNNNNNGG 2 cut(s) 190, 239
AgsI TTSAA 4 cut(s) 39, 121, 156, 214
AjnI CCWGG 1 cut(s) 238
Alw26I GTCTC 1 cut(s) 14
Ama87I CYCGRG 1 cut(s) 190
ApeKI GCWGC 1 cut(s) 235
ApoI RAATTY 1 cut(s) 106
AspS9I GGNCC 1 cut(s) 98
AsuC2I CCSGG 2 cut(s) 191, 192
AsuHPI GGTGA 1 cut(s) 198
AvaI CYCGRG 1 cut(s) 190
AvaII GGWCC 1 cut(s) 98
BbvI GCAGC 1 cut(s) 247
BciT130I CCWGG 1 cut(s) 240
BcnI CCSGG 2 cut(s) 191, 192
BcoDI GTCTC 1 cut(s) 14
BfaI CTAG 1 cut(s) 102
BisI GCNGC 1 cut(s) 236
BlsI GCNGC 1 cut(s) 237
Bme1390I CCNGG 3 cut(s) 191, 192, 240
Bme18I GGWCC 1 cut(s) 98
BmeT110I CYCGRG 1 cut(s) 190
BmgT120I GGNCC 1 cut(s) 98
BmiI GGNNCC 1 cut(s) 99
BmrFI CCNGG 3 cut(s) 191, 192, 240
BmsI GCATC 1 cut(s) 39
BplI GAGNNNNNCTC 2 cut(s) 37, 69
BpmI CTGGAG 1 cut(s) 261
BpuMI CCSGG 2 cut(s) 191, 192
BsaBI GATNNNNATC 1 cut(s) 29
BsaJI CCNNGG 2 cut(s) 189, 190
BsaXI ACNNNNNCTCC 4 cut(s) 11, 41, 164, 194
Bsc4I CCNNNNNNNGG 2 cut(s) 190, 239
Bse8I GATNNNNATC 1 cut(s) 29
BseBI CCWGG 1 cut(s) 240
BseDI CCNNGG 2 cut(s) 189, 190
BseJI GATNNNNATC 1 cut(s) 29
BseLI CCNNNNNNNGG 2 cut(s) 190, 239
BseMII CTCAG 1 cut(s) 68
BseXI GCAGC 1 cut(s) 247
BsiHKCI CYCGRG 1 cut(s) 190
BsiSI CCGG 1 cut(s) 191
BslFI GGGAC 1 cut(s) 111
BslI CCNNNNNNNGG 2 cut(s) 190, 239
BsmAI GTCTC 1 cut(s) 14
BsmBI CGTCTC 1 cut(s) 14
BsmFI GGGAC 1 cut(s) 111
BsoBI CYCGRG 1 cut(s) 190
Bsp143I GATC 1 cut(s) 3
BspACI CCGC 1 cut(s) 233
BspCNI CTCAG 1 cut(s) 67
BspLI GGNNCC 1 cut(s) 99
BssECI CCNNGG 2 cut(s) 189, 190
BssMI GATC 1 cut(s) 3
Bst2UI CCWGG 1 cut(s) 240
Bst4CI ACNGT 1 cut(s) 226
BstDEI CTNAG 1 cut(s) 54
BstKTI GATC 1 cut(s) 6
BstMAI GTCTC 1 cut(s) 14
BstMBI GATC 1 cut(s) 3
BstNI CCWGG 1 cut(s) 240
BstSCI CCNGG 3 cut(s) 189, 190, 238
BstV1I GCAGC 1 cut(s) 247
BtsIMutI CAGTG 1 cut(s) 132
Cfr13I GGNCC 1 cut(s) 98
Cfr9I CCCGGG 1 cut(s) 190
Csp6I GTAC 1 cut(s) 217
CviAII CATG 1 cut(s) 70
CviJI RGCY 2 cut(s) 42, 238
CviKI_1 RGCY 2 cut(s) 42, 238
CviQI GTAC 1 cut(s) 217
DdeI CTNAG 1 cut(s) 54
DpnI GATC 1 cut(s) 5
DpnII GATC 1 cut(s) 3
Eco47I GGWCC 1 cut(s) 98
Eco57I CTGAAG 1 cut(s) 156
Eco88I CYCGRG 1 cut(s) 190
EcoO109I RGGNCCY 1 cut(s) 98
EcoRII CCWGG 1 cut(s) 238
Esp3I CGTCTC 1 cut(s) 14
FaeI CATG 1 cut(s) 73
FaiI YATR 3 cut(s) 71, 79, 198
FaqI GGGAC 1 cut(s) 111
FatI CATG 1 cut(s) 69
Fnu4HI GCNGC 1 cut(s) 236
Fsp4HI GCNGC 1 cut(s) 236
FspBI CTAG 1 cut(s) 102
GluI GCNGC 1 cut(s) 236
GsuI CTGGAG 1 cut(s) 261
HapII CCGG 1 cut(s) 191
Hin1II CATG 1 cut(s) 73
HinfI GANTC 2 cut(s) 117, 160
HpaII CCGG 1 cut(s) 191
HphI GGTGA 1 cut(s) 198
Hpy166II GTNNAC 1 cut(s) 202
Hpy188I TCNGA 3 cut(s) 57, 92, 263
Hpy8I GTNNAC 1 cut(s) 202
HpyAV CCTTC 3 cut(s) 55, 97, 187
HpyCH4III ACNGT 1 cut(s) 226
HpyCH4V TGCA 2 cut(s) 30, 166
HpyF3I CTNAG 1 cut(s) 54
Hsp92II CATG 1 cut(s) 73
Kzo9I GATC 1 cut(s) 3
LpnPI CCDG 4 cut(s) 56, 204, 225, 252
Lsp1109I GCAGC 1 cut(s) 247
LweI GCATC 1 cut(s) 39
MaeI CTAG 1 cut(s) 102
MaeIII GTNAC 1 cut(s) 65
MalI GATC 1 cut(s) 5
MboI GATC 1 cut(s) 3
MboII GAAGA 1 cut(s) 70
MluCI AATT 1 cut(s) 106
MnlI CCTC 2 cut(s) 17, 41
MspI CCGG 1 cut(s) 191
MspR9I CCNGG 3 cut(s) 191, 192, 240
MvaI CCWGG 1 cut(s) 240
NciI CCSGG 2 cut(s) 191, 192
NdeII GATC 1 cut(s) 3
NlaIII CATG 1 cut(s) 73
NlaIV GGNNCC 1 cut(s) 99
PfeI GAWTC 2 cut(s) 117, 160
PkrI GCNGC 1 cut(s) 237
PpuMI RGGWCCY 1 cut(s) 98
Psp5II RGGWCCY 1 cut(s) 98
Psp6I CCWGG 1 cut(s) 238
PspGI CCWGG 1 cut(s) 238
PspN4I GGNNCC 1 cut(s) 99
PspPI GGNCC 1 cut(s) 98
PspPPI RGGWCCY 1 cut(s) 98
RsaI GTAC 1 cut(s) 218
RsaNI GTAC 1 cut(s) 217
SatI GCNGC 1 cut(s) 236
Sau3AI GATC 1 cut(s) 3
Sau96I GGNCC 1 cut(s) 98
ScrFI CCNGG 3 cut(s) 191, 192, 240
SetI ASST 3 cut(s) 52, 103, 211
SfaNI GCATC 1 cut(s) 39
SinI GGWCC 1 cut(s) 98
SmaI CCCGGG 1 cut(s) 192
Sse9I AATT 1 cut(s) 106
SsiI CCGC 1 cut(s) 233
SspMI CTAG 1 cut(s) 102
StyD4I CCNGG 3 cut(s) 189, 190, 238
TaaI ACNGT 1 cut(s) 226
TasI AATT 1 cut(s) 106
TatI WGTACW 1 cut(s) 216
TfiI GAWTC 2 cut(s) 117, 160
TscAI CASTG 1 cut(s) 139
TseI GCWGC 1 cut(s) 235
TspGWI ACGGA 1 cut(s) 218
TspMI CCCGGG 1 cut(s) 190
TspRI CASTG 1 cut(s) 139
VpaK11BI GGWCC 1 cut(s) 98
XapI RAATTY 1 cut(s) 106
XmaI CCCGGG 1 cut(s) 190
XspI CTAG 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.