FvH4_4g30210

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016506
Physical Location & Seq
Reverse (-)
29958332 .. 29960439
2108 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g30210.t1

Sequence Viewer

Length: 792 bp
ATGTCGTCGCCCGAGAAGGAGAGAGAGACCCAGGTTTACATGGCCAAGCTCGCCGAGCAGGCTGAACGCTACGAAGAAATGGTAGAGTGTATGAAGAAAGTTGCGAAACTTGATCTTGAGCTGACTGTGGAGGAGAGGAATCTTCTGTCTGTGGGATACAAAAATGTGATAGGTGCTCGAAGGGCCTCGTGGCGCATTATGTCTTCGATTGAGCAAAAGGAAGAGTCTAAGGGGAATGAGCACAATGTCAAACTGATCAAGAGTTACCGCCAGAAGGTGGAGGAGGAACTCTCCAAGATTTGCAGTGACATTCTCACTATCATTGACAAGCACCTCATTCCCTCTTCCAGCTCCGGAGAGGCCACTGTTTTCTATTACAAGATGAAAGGTGACTATTACCGGTACCTTGCTGAGTTCAAGACTGAACAGGACAGAAAAGAGGCAGCTGAACAGTCACTCAAGGGATATGAGGCTGCTTCAGCCACTGCAAACACAGATCTACCATCGACCCACCCAATCCGTCTTGGTCTAGCTCTCAACTTTTCTGTCTTCTACTATGAGATTATGAATTCTCCTGAGAGGGCTTGTCATTTGGCTAAACAAGCTTTTGATGAGGCAATTGCAGAGTTGGACACCTTGAGTGAGGAGTCATACAAGGACAGCACCCTTATCATGCAATTGTTGAGAGACAACCTCACTCTCTGGACCTCTGATTTGCCTGAAGATGGAGGTGATGACAATTTTAAAGCCGAAGAAAGCAAATCTACCGAGCCTAATTCTGCTGCGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

264

Amino Acids

29.96

Weight (kDa)

4.92

Isoelectric Point (pI)

55.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 14 - 236 1.6e-108 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016506)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26480
fragaria_vesca FvH4_4g30210
malus_domestica MD13G1063600.v1.1 MD16G1062100.v1.1
prunus_persica Prupe.1G285800_v2.0.a1
pyrus_communis pycom13g05620
rosa_chinensis RchiOBHm_Chr4g0438531
rosa_laevigata RLG00000006354
rosa_multiflora Rmu_sc0002327.1_g000007
rosa_roxburghii Rroxscaffold_5G00379530
rosa_rugosa Rorug04G0311100
rosa_samantha Rh4DG367600
rosa_wichuraiana Rw0G006620 Rw0G016570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 402
AccB1I GGYRCC 1 cut(s) 402
AccB7I CCANNNNNTGG 1 cut(s) 277
AccIII TCCGGA 1 cut(s) 353
AciI CCGC 1 cut(s) 268
AcoI YGGCCR 1 cut(s) 42
AcsI RAATTY 1 cut(s) 568
AcuI CTGAAG 2 cut(s) 462, 741
AfaI GTAC 1 cut(s) 404
AfiI CCNNNNNNNGG 3 cut(s) 274, 277, 725
AgeI ACCGGT 1 cut(s) 399
AgsI TTSAA 1 cut(s) 418
AjnI CCWGG 1 cut(s) 30
AluBI AGCT 6 cut(s) 49, 121, 351, 446, 533, 605
AluI AGCT 6 cut(s) 49, 121, 351, 446, 533, 605
Alw21I GWGCWC 2 cut(s) 178, 243
Alw26I GTCTC 2 cut(s) 20, 681
AlwNI CAGNNNCTG 1 cut(s) 485
Ama87I CYCGRG 1 cut(s) 11
Aor13HI TCCGGA 1 cut(s) 353
AoxI GGCC 3 cut(s) 42, 183, 360
ApeKI GCWGC 3 cut(s) 443, 473, 782
ApoI RAATTY 1 cut(s) 568
ArsI GACNNNNNNTTYG 2 cut(s) 697, 729
AsiGI ACCGGT 1 cut(s) 399
Asp718I GGTACC 1 cut(s) 402
AspLEI GCGC 1 cut(s) 195
AspS9I GGNCC 2 cut(s) 183, 705
AsuHPI GGTGA 2 cut(s) 401, 743
AvaI CYCGRG 1 cut(s) 11
AvaII GGWCC 1 cut(s) 705
BalI TGGCCA 1 cut(s) 44
BanI GGYRCC 1 cut(s) 402
BauI CACGAG 1 cut(s) 187
BbsI GAAGAC 2 cut(s) 195, 541
Bbv12I GWGCWC 2 cut(s) 178, 243
BbvI GCAGC 3 cut(s) 455, 460, 769
BccI CCATC 2 cut(s) 511, 719
BciT130I CCWGG 1 cut(s) 32
BciVI GTATCC 1 cut(s) 149
BclI TGATCA 1 cut(s) 255
BcoDI GTCTC 2 cut(s) 20, 681
BfaI CTAG 1 cut(s) 530
BfuI GTATCC 1 cut(s) 149
BglI GCCNNNNNGGC 1 cut(s) 59
BglII AGATCT 1 cut(s) 496
BisI GCNGC 3 cut(s) 444, 474, 783
BlsI GCNGC 3 cut(s) 445, 475, 784
Bme1390I CCNGG 1 cut(s) 32
Bme18I GGWCC 1 cut(s) 705
BmeT110I CYCGRG 1 cut(s) 11
BmgT120I GGNCC 2 cut(s) 183, 705
BmiI GGNNCC 1 cut(s) 404
BmrFI CCNGG 1 cut(s) 32
BpiI GAAGAC 2 cut(s) 195, 541
BplI GAGNNNNNCTC 4 cut(s) 275, 307, 678, 710
BpuEI CTTGAG 3 cut(s) 137, 443, 658
BsaI GGTCTC 1 cut(s) 20
BsaJI CCNNGG 1 cut(s) 30
BsaWI WCCGGW 2 cut(s) 353, 399
Bsc4I CCNNNNNNNGG 3 cut(s) 274, 277, 725
Bse118I RCCGGY 1 cut(s) 399
BseAI TCCGGA 1 cut(s) 353
BseBI CCWGG 1 cut(s) 32
BseDI CCNNGG 1 cut(s) 30
BseLI CCNNNNNNNGG 3 cut(s) 274, 277, 725
BseMII CTCAG 2 cut(s) 402, 567
BseRI GAGGAG 3 cut(s) 146, 296, 659
BseXI GCAGC 3 cut(s) 455, 460, 769
BshFI GGCC 3 cut(s) 44, 185, 362
BshNI GGYRCC 1 cut(s) 402
BshTI ACCGGT 1 cut(s) 399
BsiHKAI GWGCWC 2 cut(s) 178, 243
BsiHKCI CYCGRG 1 cut(s) 11
BsiSI CCGG 2 cut(s) 354, 400
BslI CCNNNNNNNGG 3 cut(s) 274, 277, 725
BsmAI GTCTC 2 cut(s) 20, 681
BsnI GGCC 3 cut(s) 44, 185, 362
Bso31I GGTCTC 1 cut(s) 20
BsoBI CYCGRG 1 cut(s) 11
Bsp1286I GDGCHC 2 cut(s) 178, 243
Bsp13I TCCGGA 1 cut(s) 353
Bsp143I GATC 3 cut(s) 112, 255, 496
BspACI CCGC 1 cut(s) 268
BspANI GGCC 3 cut(s) 44, 185, 362
BspCNI CTCAG 2 cut(s) 403, 568
BspEI TCCGGA 1 cut(s) 353
BspLI GGNNCC 1 cut(s) 404
BspT107I GGYRCC 1 cut(s) 402
BspTNI GGTCTC 1 cut(s) 20
BsrFI RCCGGY 1 cut(s) 399
BssAI RCCGGY 1 cut(s) 399
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 3 cut(s) 112, 255, 496
BssSI CACGAG 1 cut(s) 187
Bst2BI CACGAG 1 cut(s) 187
Bst2UI CCWGG 1 cut(s) 32
Bst4CI ACNGT 3 cut(s) 127, 367, 453
Bst6I CTCTTC 2 cut(s) 216, 349
BstC8I GCNNGC 2 cut(s) 51, 60
BstDEI CTNAG 3 cut(s) 228, 411, 576
BstHHI GCGC 1 cut(s) 195
BstKTI GATC 3 cut(s) 115, 258, 499
BstMAI GTCTC 2 cut(s) 20, 681
BstMBI GATC 3 cut(s) 112, 255, 496
BstMWI GCNNNNNNNGC 6 cut(s) 50, 55, 59, 182, 479, 602
BstNI CCWGG 1 cut(s) 32
BstSCI CCNGG 1 cut(s) 30
BstV1I GCAGC 3 cut(s) 455, 460, 769
BstV2I GAAGAC 2 cut(s) 195, 541
BstX2I RGATCY 1 cut(s) 496
BstYI RGATCY 1 cut(s) 496
BsuI GTATCC 1 cut(s) 149
BsuRI GGCC 3 cut(s) 44, 185, 362
BtsI GCAGTG 2 cut(s) 310, 483
BtsIMutI CAGTG 3 cut(s) 310, 363, 483
Cac8I GCNNGC 2 cut(s) 51, 60
CaiI CAGNNNCTG 1 cut(s) 485
CfoI GCGC 1 cut(s) 195
Cfr10I RCCGGY 1 cut(s) 399
Cfr13I GGNCC 2 cut(s) 183, 705
Csp6I GTAC 1 cut(s) 403
CspAI ACCGGT 1 cut(s) 399
CviAII CATG 2 cut(s) 40, 673
CviQI GTAC 1 cut(s) 403
DdeI CTNAG 3 cut(s) 228, 411, 576
DpnI GATC 3 cut(s) 114, 257, 498
DpnII GATC 3 cut(s) 112, 255, 496
DraI TTTAAA 1 cut(s) 745
EaeI YGGCCR 1 cut(s) 42
Eam1104I CTCTTC 2 cut(s) 216, 349
EarI CTCTTC 2 cut(s) 216, 349
Eco31I GGTCTC 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 705
Eco57I CTGAAG 2 cut(s) 462, 741
Eco88I CYCGRG 1 cut(s) 11
EcoO109I RGGNCCY 1 cut(s) 183
EcoRI GAATTC 1 cut(s) 568
EcoRII CCWGG 1 cut(s) 30
FaeI CATG 2 cut(s) 43, 676
FaiI YATR 8 cut(s) 41, 92, 200, 468, 558, 566, 652, 674
FatI CATG 2 cut(s) 39, 672
FbaI TGATCA 1 cut(s) 255
Fnu4HI GCNGC 3 cut(s) 444, 474, 783
Fsp4HI GCNGC 3 cut(s) 444, 474, 783
FspBI CTAG 1 cut(s) 530
GlaI GCGC 1 cut(s) 194
GluI GCNGC 3 cut(s) 444, 474, 783
HaeIII GGCC 3 cut(s) 44, 185, 362
HapII CCGG 2 cut(s) 354, 400
HhaI GCGC 1 cut(s) 195
Hin1II CATG 2 cut(s) 43, 676
Hin6I GCGC 1 cut(s) 193
HinP1I GCGC 1 cut(s) 193
HindIII AAGCTT 1 cut(s) 603
HinfI GANTC 3 cut(s) 139, 224, 647
HpaII CCGG 2 cut(s) 354, 400
HphI GGTGA 2 cut(s) 401, 743
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 1 cut(s) 712
Hpy188III TCNNGA 6 cut(s) 116, 259, 354, 418, 575, 703
Hpy8I GTNNAC 1 cut(s) 37
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 3 cut(s) 10, 174, 268
HpyCH4III ACNGT 3 cut(s) 127, 367, 453
HpyCH4V TGCA 4 cut(s) 303, 488, 623, 676
HpyF10VI GCNNNNNNNGC 6 cut(s) 50, 55, 59, 182, 479, 602
HpyF3I CTNAG 3 cut(s) 228, 411, 576
Hsp92II CATG 2 cut(s) 43, 676
HspAI GCGC 1 cut(s) 193
Kpn2I TCCGGA 1 cut(s) 353
KpnI GGTACC 1 cut(s) 406
Ksp22I TGATCA 1 cut(s) 255
Kzo9I GATC 3 cut(s) 112, 255, 496
LmnI GCTCC 1 cut(s) 356
Lsp1109I GCAGC 3 cut(s) 455, 460, 769
MaeI CTAG 1 cut(s) 530
MaeIII GTNAC 4 cut(s) 263, 305, 389, 453
MalI GATC 3 cut(s) 114, 257, 498
MboI GATC 3 cut(s) 112, 255, 496
MboII GAAGA 9 cut(s) 86, 106, 134, 195, 233, 336, 541, 734, 764
MfeI CAATTG 2 cut(s) 618, 677
MflI RGATCY 1 cut(s) 496
MhlI GDGCHC 2 cut(s) 178, 243
MlsI TGGCCA 1 cut(s) 44
MluCI AATT 5 cut(s) 568, 618, 677, 739, 775
MluNI TGGCCA 1 cut(s) 44
MlyI GAGTC 2 cut(s) 233, 656
MmeI TCCRAC 1 cut(s) 609
Mox20I TGGCCA 1 cut(s) 44
MroI TCCGGA 1 cut(s) 353
MscI TGGCCA 1 cut(s) 44
MseI TTAA 1 cut(s) 744
Msp20I TGGCCA 1 cut(s) 44
MspA1I CMGCKG 1 cut(s) 446
MspI CCGG 2 cut(s) 354, 400
MspR9I CCNGG 1 cut(s) 32
MunI CAATTG 2 cut(s) 618, 677
MvaI CCWGG 1 cut(s) 32
MwoI GCNNNNNNNGC 6 cut(s) 50, 55, 59, 182, 479, 602
NdeII GATC 3 cut(s) 112, 255, 496
NlaIII CATG 2 cut(s) 43, 676
NlaIV GGNNCC 1 cut(s) 404
NmeAIII GCCGAG 1 cut(s) 79
NmuCI GTSAC 3 cut(s) 305, 389, 453
PfeI GAWTC 1 cut(s) 139
PflMI CCANNNNNTGG 1 cut(s) 277
PinAI ACCGGT 1 cut(s) 399
PkrI GCNGC 3 cut(s) 445, 475, 784
PleI GAGTC 2 cut(s) 232, 655
PpsI GAGTC 2 cut(s) 232, 655
Psp6I CCWGG 1 cut(s) 30
PspGI CCWGG 1 cut(s) 30
PspN4I GGNNCC 1 cut(s) 404
PspPI GGNCC 2 cut(s) 183, 705
PstNI CAGNNNCTG 1 cut(s) 485
PsuI RGATCY 1 cut(s) 496
PvuII CAGCTG 1 cut(s) 446
RsaI GTAC 1 cut(s) 404
RsaNI GTAC 1 cut(s) 403
SaqAI TTAA 1 cut(s) 744
SatI GCNGC 3 cut(s) 444, 474, 783
Sau3AI GATC 3 cut(s) 112, 255, 496
Sau96I GGNCC 2 cut(s) 183, 705
SchI GAGTC 2 cut(s) 233, 656
ScrFI CCNGG 1 cut(s) 32
SduI GDGCHC 2 cut(s) 178, 243
SinI GGWCC 1 cut(s) 705
SmlI CTYRAG 3 cut(s) 116, 458, 637
SmoI CTYRAG 3 cut(s) 116, 458, 637
Sse9I AATT 5 cut(s) 568, 618, 677, 739, 775
SsiI CCGC 1 cut(s) 268
SspMI CTAG 1 cut(s) 530
StyD4I CCNGG 1 cut(s) 30
TaaI ACNGT 3 cut(s) 127, 367, 453
TaqI TCGA 3 cut(s) 178, 206, 506
TasI AATT 5 cut(s) 568, 618, 677, 739, 775
TfiI GAWTC 1 cut(s) 139
Tru1I TTAA 1 cut(s) 744
Tru9I TTAA 1 cut(s) 744
TscAI CASTG 3 cut(s) 310, 370, 490
TseFI GTSAC 3 cut(s) 305, 389, 453
TseI GCWGC 3 cut(s) 443, 473, 782
Tsp45I GTSAC 3 cut(s) 305, 389, 453
TspDTI ATGAA 3 cut(s) 107, 398, 581
TspGWI ACGGA 1 cut(s) 509
TspRI CASTG 3 cut(s) 310, 370, 490
Van91I CCANNNNNTGG 1 cut(s) 277
VpaK11BI GGWCC 1 cut(s) 705
XapI RAATTY 1 cut(s) 568
XspI CTAG 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.